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You searched for +publisher:"Universidade Nova" +contributor:("Bento, Isabel"). Showing records 1 – 2 of 2 total matches.

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Universidade Nova

1. Silva, Catarina Isabel Simões Pires. STRUCTURE-FUNCTION ANALYSIS OF MULTI-COPPER OXIDASES.

Degree: 2013, Universidade Nova

Dissertation presented to obtain the Ph.D degree in Biochemistry, Structural Biochemistry

Dioxygen is a ubiquitous electron acceptor in aerobic biological systems, playing a vital role in many biological processes upon its activation and reduction. However, the precise routes through which such mechanisms occur are still poorly understood for many enzyme families. The work presented in this dissertation focuses on Multi-copper oxidases (MCOs), a family of enzymes able to couple the one-electron oxidation of a vast array of substrate molecules with the four-electron reduction of dioxygen to water.(...)

Advisors/Committee Members: Bento, Isabel.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Silva, C. I. S. P. (2013). STRUCTURE-FUNCTION ANALYSIS OF MULTI-COPPER OXIDASES. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/11966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Silva, Catarina Isabel Simões Pires. “STRUCTURE-FUNCTION ANALYSIS OF MULTI-COPPER OXIDASES.” 2013. Thesis, Universidade Nova. Accessed July 23, 2019. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/11966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Silva, Catarina Isabel Simões Pires. “STRUCTURE-FUNCTION ANALYSIS OF MULTI-COPPER OXIDASES.” 2013. Web. 23 Jul 2019.

Vancouver:

Silva CISP. STRUCTURE-FUNCTION ANALYSIS OF MULTI-COPPER OXIDASES. [Internet] [Thesis]. Universidade Nova; 2013. [cited 2019 Jul 23]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/11966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silva CISP. STRUCTURE-FUNCTION ANALYSIS OF MULTI-COPPER OXIDASES. [Thesis]. Universidade Nova; 2013. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/11966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Universidade Nova

2. Moutinho, Daniela Mesquita. Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization.

Degree: 2013, Universidade Nova

Dissertation to obtain a Master Degree in Molecular Genetics and Biomedicine at Faculty of Sciences and Technology,Universidade Nova de Lisboa

Human ceruloplasmin (hCp) is the molecular linker between the copper and iron metabolism and its importance in the homeostasis of human body has been implied in some neurological diseases. This plasma cuproenzyme has ferroxidase activity, oxidizing Fe2+ to Fe3+ and incorporating it into apotransferrin. hCp also has aminoxidase activity regulating the levels of amine stress hormones in the bloodstream and brain. Thus, it is thought to have an important role in neurodegenerative diseases such as Alzheimer’s or Parkinson’s. To know more about the role of cerulopalsmin on the oxidation of neurotransmitters and on brain homeostasis it is essential to know which protein residues are implied in the binding and stabilization of these neurotransmitters. The primary source of structural information for protein-ligand complexes is X-ray crystallography. This is the most successful method to determine macromolecular 3D structures but has some limitations as obtaining good diffracting protein crystals. In this study several attempts were made to achieve better hCp diffracting crystals and crystals of hcp in complex with dopamine, L-dopa, epinephrine or serotonin in order to further determine its tridimensional structure. To improve hCp stabilization and solubility, differential scanning fluorimetry and dynamic light scattering were used in a search for a better buffer for crystallization. For hCp crystallization the vapour-diffusion technique was used in combination with several other methods. Commercial crystallization screens, crystal seeding, additives, crosslinking were the several methods used to improve crystal diffraction. Co-crystallization of hCp with neurotransmitters was performed with no success. Soaking of hCp crystals with the neurotransmitters was performed in an attempt to get crystals of the hCpneurotransmitter complexes. All crystals were sent for analysis at European Synchrotron Radiation Facility (ESRF) and structural data will be further processed.

Advisors/Committee Members: Bento, Isabel, Sampaio, José Paulo.

Subjects/Keywords: Ceruloplasmin; Neurotransmitters; Epinephrine; Dopamine; L-dopa; Serotonin

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Moutinho, D. M. (2013). Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/10853

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Moutinho, Daniela Mesquita. “Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization.” 2013. Thesis, Universidade Nova. Accessed July 23, 2019. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/10853.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Moutinho, Daniela Mesquita. “Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization.” 2013. Web. 23 Jul 2019.

Vancouver:

Moutinho DM. Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization. [Internet] [Thesis]. Universidade Nova; 2013. [cited 2019 Jul 23]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/10853.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moutinho DM. Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization. [Thesis]. Universidade Nova; 2013. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/10853

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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