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You searched for +publisher:"Universidade Federal de Alagoas" +contributor:("http://lattes.cnpq.br/6131084470861010"). Showing records 1 – 2 of 2 total matches.

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1. Silva Filho, Benisio Ferreira da. Quantificação de fragmentos de DNA livre no sangue periférico de portadores de câncer colorretal.

Degree: 2009, Universidade Federal de Alagoas

The colorectal cancer (CRC) is the third most common malignancy in the world and in Brazil, the fifth most diagnosed and the third cause of cancer deaths. The detection of molecular markers in peripheral blood applies to the early diagnosis of cancer, before and after surgery, reducing the time of identification of CCR and improving the treatment, it is less invasive and reducing costs. This work is quantified by Real Time PCR in absolute and direct, free of DNA fragments found in the serum of patients with colorectal cancer, thereby determining values that characterize the condition of carrier of tumor. For this, a method was developed in which the measurement used as reference samples of the actual fragments ALU115 and ALU247 purified and quantified. We studied 3 major groups: Control, with healthy volunteers; surgery, patients who have already been submitted to surgical removal of colorectal tumor and non-surgery, patients who have not removed the tumor through surgery. Observing the results we note that the groups differ mainly by the values of quantification ALU247. For the control group, the limits were between 91 fentogramas and 1.55 picograms. The non operated group had amounts ranging from 8.02pg to 23.54pg and the group operators, 80fg to 5.95pg. In contrary, the results presented by quantification ALU115 did not set limits defined capable of differentiating at least one of the groups. The control group presented as limits 2pg and 69.45pg and the non operated group, 9.71pg and and 381.56pg, the group operated, 10.69pg and 196.85pg. The non operated group showed a mean significant when compared to the other two groups, but showed minor differences in the quantification ALU247 (14.62 ± 4.73 pg - p <0.05). It could be concluded that direct measurement, using reference concentrations of the fragments themselves ALU115 and ALU247 is a simple methodology, low cost and with reliable results. Moreover, the quantification ALU247 is able to identify the condition of carrier of tumor.

Fundação de Amparo a Pesquisa do Estado de Alagoas

O câncer colorretal (CCR) é a terceira neoplasia maligna mais frequente no mundo, sendo no Brasil a quinta mais diagnosticada e a terceira causa de morte por câncer. A detecção de marcadores moleculares no sangue periférico aplica-se ao diagnóstico precoce de neoplasias, antes e após procedimento cirúrgico, diminuindo assim o tempo de identificação do CCR e melhorando o tratamento, que se torna menos invasivo e diminuindo os custos. O objetivo deste trabalho é quantificar, através da PCR em Tempo Real de forma absoluta e direta, os fragmentos de DNA Livre encontrados no soro de pacientes com câncer colorretal, determinando assim valores que caracterizam a condição de portador de tumor. Para isso, foi desenvolvido um método em que a quantificação utiliza como amostras de referências os próprios fragmentos ALU115 e ALU247 purificados e quantificados. Foram estudados 3 grandes grupos: Controle, com voluntários saudáveis; Operados, pacientes que já se submeteram à cirurgia para…

Advisors/Committee Members: Silva, Luiz Antonio Ferreira da, CPF:18269427004, SILVA, L. A. F., Lins Neto, Manoel Alvaro de Freitas, CPF:16471091400, http://lattes.cnpq.br/7269775401868084, Silva Neto, Jacinto da Costa, CPF:66028647420, http://lattes.cnpq.br/6131084470861010, Silva Filho, Eurípedes Alves da, CPF:16649630497, SILVA FILHO, E. A., Tovar, Francisco Javier, CPF:02177610702, http://lattes.cnpq.br/2366497420587582.

Subjects/Keywords: DNA livre; Quantificação absoluta; Neoplasias colorretais; Free DNA; Absolute quantification; Colorectal cancer; CNPQ::CIENCIAS DA SAUDE

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APA (6th Edition):

Silva Filho, B. F. d. (2009). Quantificação de fragmentos de DNA livre no sangue periférico de portadores de câncer colorretal. (Masters Thesis). Universidade Federal de Alagoas. Retrieved from http://www.repositorio.ufal.br/handle/riufal/928

Chicago Manual of Style (16th Edition):

Silva Filho, Benisio Ferreira da. “Quantificação de fragmentos de DNA livre no sangue periférico de portadores de câncer colorretal.” 2009. Masters Thesis, Universidade Federal de Alagoas. Accessed December 04, 2020. http://www.repositorio.ufal.br/handle/riufal/928.

MLA Handbook (7th Edition):

Silva Filho, Benisio Ferreira da. “Quantificação de fragmentos de DNA livre no sangue periférico de portadores de câncer colorretal.” 2009. Web. 04 Dec 2020.

Vancouver:

Silva Filho BFd. Quantificação de fragmentos de DNA livre no sangue periférico de portadores de câncer colorretal. [Internet] [Masters thesis]. Universidade Federal de Alagoas; 2009. [cited 2020 Dec 04]. Available from: http://www.repositorio.ufal.br/handle/riufal/928.

Council of Science Editors:

Silva Filho BFd. Quantificação de fragmentos de DNA livre no sangue periférico de portadores de câncer colorretal. [Masters Thesis]. Universidade Federal de Alagoas; 2009. Available from: http://www.repositorio.ufal.br/handle/riufal/928

2. Santos Filho, Moezio de Vasconcellos Costa. Epidemiologia molecular do papilomavírus humano em uma amostra de mulheres do estado de Alagoas.

Degree: 2012, Universidade Federal de Alagoas

The Human Papillomavirus (HPV) is a virus of the Papillomaviridae family and its genome consists of DNA. It is the main cause of viral sexual transmission. Cervical cancer or uterine colon cancer is the second most common occurrence among women worldwide. Recent studies have proven that some types of HPV are mainly responsible for the development of this type of cancer. In accordance to its oncogenic activity this viral group was divided in low and high risk types. Brazil does not have a representative amount of data related to the prevalence of HPV infection. The incidence data of the virus is obtained through the analysis of carriers of the invasive carcinoma of uterine colon, cervical intraepithelial neoplasias, and others types of infections associated. Molecular assays have been showing throughout the years an excellent sensitivity and specificity in the detection of the HPV s DNA. Nowadays, in situ hybridization techniques are being used as method of choice for the detection of the viral DNA. In many studies, the Polymerase Chain Reaction (PCR) with the application of the primers MY09 and MY11 have shown efficiency in viral tracking, consequently, the development of molecular diagnostics allow monitoring of the disease, decreasing mortality rates caused by cancer of the cervix. The DNA sequencing is an efficient methodology used for the molecular characterization of the viral types, which allows the accomplishment of the alignment for similarity of the sequences obtained through others that were already identified and deposited in the GenBank. The current study s purpose was to identify the different types of HPV and the presence of co-infections through PCR and sequencing of a hyper-variable region of the L1 gene. The studied sample consisted of 515 female volunteers, which 111 (21.55%) presented the incidence of the HPV DNA. Within the acquired specimens, 50% were considered high oncogenic risk (the major incidence was types 16 and 58) and 7.21% had multiple infection. The determination of the base sequencing of the L1 gene is essential to the identification of the viral type. However, the sequencing of the complete gene and both DNA chains become financially unviable for the majority of the specialized laboratories. A practical approach applied in this study was to determine the sequencing of a specific region of the L1 gene with 450pb, where the identification of the HPV became possible by following this methodology. The techniques used showed the necessity of the application of a more sensible and specific viral diagnosis, which contributes to the viral infection control and to the reduction of the mortality rate caused by cervical cancer.

Fundação de Amparo a Pesquisa do Estado de Alagoas

O Papilomavírus Humano (HPV) é um vírus da família Papillomaviridae e possui o seu genoma constituído de DNA. É o causador da transmissão sexual viral de maior frequência. O câncer cervical ou câncer de colo de útero possui a segunda maior ocorrência nas mulheres de todo o mundo. Estudos recentes têm comprovado que…

Advisors/Committee Members: Silva, Luiz Antonio Ferreira da, CPF:18269427004, SILVA, L. A. F., Azevedo, Dalmo Almeida de, CPF:49935275949, http://lattes.cnpq.br/4202083703695616, Tovar, Francisco Javier, CPF:02177610702, http://lattes.cnpq.br/2366497420587582, Silva Neto, Jacinto da Costa, CPF:66028647420, http://lattes.cnpq.br/6131084470861010.

Subjects/Keywords: Papilomavírus humano (HPV); Gene L1; Reação em cadeia da polimerase (PCR); Sequenciamento de DNA; GenBank; Human Papillomavirus (HPV); L1 gene; Polymerase Chain Reaction (PCR); DNA Sequencing; GenBank; CNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA::EPIDEMIOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Santos Filho, M. d. V. C. (2012). Epidemiologia molecular do papilomavírus humano em uma amostra de mulheres do estado de Alagoas. (Masters Thesis). Universidade Federal de Alagoas. Retrieved from http://www.repositorio.ufal.br/handle/riufal/948

Chicago Manual of Style (16th Edition):

Santos Filho, Moezio de Vasconcellos Costa. “Epidemiologia molecular do papilomavírus humano em uma amostra de mulheres do estado de Alagoas.” 2012. Masters Thesis, Universidade Federal de Alagoas. Accessed December 04, 2020. http://www.repositorio.ufal.br/handle/riufal/948.

MLA Handbook (7th Edition):

Santos Filho, Moezio de Vasconcellos Costa. “Epidemiologia molecular do papilomavírus humano em uma amostra de mulheres do estado de Alagoas.” 2012. Web. 04 Dec 2020.

Vancouver:

Santos Filho MdVC. Epidemiologia molecular do papilomavírus humano em uma amostra de mulheres do estado de Alagoas. [Internet] [Masters thesis]. Universidade Federal de Alagoas; 2012. [cited 2020 Dec 04]. Available from: http://www.repositorio.ufal.br/handle/riufal/948.

Council of Science Editors:

Santos Filho MdVC. Epidemiologia molecular do papilomavírus humano em uma amostra de mulheres do estado de Alagoas. [Masters Thesis]. Universidade Federal de Alagoas; 2012. Available from: http://www.repositorio.ufal.br/handle/riufal/948

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