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You searched for +publisher:"Universidade Federal de Alagoas" +contributor:("CPF:75900220471"). Showing records 1 – 2 of 2 total matches.

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1. Souza, Aryanna Kelly Pinheiro. Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL.

Degree: 2009, Universidade Federal de Alagoas

The fungi are being opportunistic, with wide distribution in nature, including the hospital environment, where it is frequent the presence of microorganisms that cause hospital infection, especially in immunocompromised patients. To identify and quantify the fungal microbiota in air, the filters of air-conditioners and samples of newborns from the neonatal ICU at University Hospital / HUPA, Maceió, Alagoas. Were held 20 exhibitions of collections totaling 400 disposable Petri dishes containing Sabouraud agar medium plus antibiotic. The plates were placed randomly inside and open the two neonatal ICUs (A and B) for 20 minutes. Also, there were 20 samples of the filters of air-conditioners of neonatal ICUs and conducted the direct examination and culture of clinical samples from newborns. CFU were isolated in 1209, these 675 (55.8%) were isolated CFU before cleaning to be done in the neonatal ICU and 534 (44.2%) CFU obtained after cleaning. Of 697 CFU of the environment of the NICU, 372 were isolated before and 325 after cleaning. Environment B, were obtained of 512 CFU and 303 CFU were obtained before cleaning and 209 after UFC. During the study species of Cladosporium was the most representative in the air and in the filters of air-conditioners. The clinical sample was more representative urine with 79.5%. Among the identified yeast Candida parapsilosis was present in 38.9% of environmental isolates of yeast and Candida albicans was isolated in 20% of clinical samples of newborns. The presence of pathogenic fungi in the two environments of the neonatal ICU and clinical samples of infants, demonstrating the need for constant monitoring to take control of microorganisms in hospital environments, especially in intensive care units where there is the presence of immunocompromised patients prone to develop fungal infections.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Os fungos que são dispersos pelo ar atmosférico são denominados de fungos anemófilos e são importantes por causar patologias em pacientes imunocomprometidos. Com o objetivo de monitorar a microbiota fúngica do ar, dos filtros dos condicionadores de ar e das amostras clínicas dos recémnascidos internados na UTI neonatal do Hospital Universitário de Maceió - AL foram realizadas coletas de amostras do ar através da técnica de exposição de placas de Petri contendo o meio ágar Sabouraud acrescido de antibiótico. O material sólido dos filtros dos condicionadores de ar foi coletado utilizando um swab esterilizado. E das amostras clínicas dos recém-nascidos foram realizados o exame direto e cultura. Para identificação dos fungos filamentos foram comparadas as características macro e microscópicas e as leveduras foram identificados através da técnica de PCR. Entre as 400 exposições de placas realizadas foram isoladas 1029 UFC, sendo 675 (55,8%) UFC obtidas antes e 534 (44,2%) após a limpeza. Das 40 amostras analisadas dos filtros dos condicionadores de ar foram obtidas 303 UFC, sendo o fungo Cladosporium cladosporioides com maior frequência…

Advisors/Committee Members: Silva Filho, Eurípedes Alves da, CPF:16649630497, SILVA FILHO, E. A., Araújo, Maria Anilda dos Santos, CPF:75900220471, ARAÚJO, M. A. S., Magalhães, Oliane Maria Correia, CPF:55040497415, MAGALHÃES, O. M. C., Herrmann, Délia Maria de Moura Lima, CPF:31012973468, HERRMANN, D. M. M. L., Silva, Luiz Antonio Ferreira da, CPF:18269427004, SILVA, L. A. F..

Subjects/Keywords: Fungos; Infecção hospitalar; UTI neonatal; Limpeza; Fungi; Hospital infection; Neonatal ICU; Cleaning; CNPQ::CIENCIAS DA SAUDE

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APA (6th Edition):

Souza, A. K. P. (2009). Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL. (Masters Thesis). Universidade Federal de Alagoas. Retrieved from http://www.repositorio.ufal.br/handle/riufal/920

Chicago Manual of Style (16th Edition):

Souza, Aryanna Kelly Pinheiro. “Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL.” 2009. Masters Thesis, Universidade Federal de Alagoas. Accessed November 28, 2020. http://www.repositorio.ufal.br/handle/riufal/920.

MLA Handbook (7th Edition):

Souza, Aryanna Kelly Pinheiro. “Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL.” 2009. Web. 28 Nov 2020.

Vancouver:

Souza AKP. Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL. [Internet] [Masters thesis]. Universidade Federal de Alagoas; 2009. [cited 2020 Nov 28]. Available from: http://www.repositorio.ufal.br/handle/riufal/920.

Council of Science Editors:

Souza AKP. Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL. [Masters Thesis]. Universidade Federal de Alagoas; 2009. Available from: http://www.repositorio.ufal.br/handle/riufal/920

2. Lima, Gabryelle Barbosa Cordeiro de. Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal.

Degree: 2012, Universidade Federal de Alagoas

Candida species have emerged as the second most common cause of vulvovaginitis in the world, producing infections that can cause unwanted symptoms such as itching, discharge, dyspareunia and burning. The objective of this study was to evaluate the genetic variability and the antifungal susceptibility profile of Candida yeasts isolated from vaginal secretions of patients Maceió - Alagoas. The yeasts were collected weekly in the sector of five clinical microbiology laboratories located in the city of Maceió between March and December 2010. For each isolate was performed through a purification of seeding a suspension of yeast in YPD solid medium. DNA extraction was performed by phenol / chloroform and identification by PCR with primers species specific for C.albicans, C. glabrata, C. tropicalis, C. krusei and C. parapsilosis. The patients with vaginal candidiasis were evaluated for the presence of symptoms and risk factors for acquiring the disease. The patterns of sensitivity to ketoconazole, fluconazole, itraconazole and amphotericin B were evaluated according to CLSI standards (M27-A2).Molecular typing was used in the microsatellite (GTG) 5 and the fragments separated on 1.5% agarose gel at 75 volts / cm for 120 minutes, stained with ethidium bromide and viewed on photodocumentation system. Banding patterns were analyzed by BioNumerics 6.5 soltware to obtain electropherogram using the similarity coefficient Jacard. We obtained 296 isolates of Candida yeasts, and C. albicans (82.45%) the most frequent species, followed by C. glabrata with 7.75%, C. parapsilosis with 4.05%, C. tropicalis witn 3.75% and C. krusei 2%. These isolates exhibited resistance profile of 66,7% for amphotericin B, fluconazole for 52,6%, 89,7% for itraconazole and to ketoconazole 21,3%. Among the risk factors for acquiring the disease stands out pregnancy with 24.66% and history of VVC in 15.52%. The main clinical sign was presented discharge in 77,13% of cases. The species analyzed showed a high diversity for the genetic marker (GTG)5, with values ranging from 0.7470 to 0.9963, which allowed the formation of genetic patterns 172 for C. albicans, 10 for C. tropicalis, 11 for C. parapsilosis, 8 for C. glabrata and 4 for C. krusei. The incidence of Candida species with a profile of resistance to itracozaol, amphotericin B, fluconazole and ketoconazole, provides epidemiological data for the study area and reinforces the importance of identifying yeasts to the species level and testing of in vitro antifungal susceptibility.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

As espécies de Candida têm emergido como a segunda causa mais comum de vulvovaginites no mundo, produzindo infecções, que podem causar sintomas indesejáveis como, prurido, corrimento, dispareunia e ardor. O objetivo desse estudo foi avaliar a variabilidade genética e o perfil de susceptibilidade aos antifúngicos em leveduras do gênero Candida isoladas de secreção vaginal de pacientes de Maceió Alagoas. As amostras foram coletadas no setor de microbiologia clínica…

Advisors/Committee Members: Silva Filho, Eurípedes Alves da, CPF:16649630497, SILVA FILHO, E. A., Azevedo, Dalmo Almeida de, CPF:49935275949, http://lattes.cnpq.br/4202083703695616, Neves, Rejane Pereira, CPF:21401365434, http://lattes.cnpq.br/0360951033804105, Araújo, Maria Anilda dos Santos, CPF:75900220471, ARAÚJO, M. A. S..

Subjects/Keywords: Candida; Susceptibilidade aos antifúngicos; PCR; Caracterização genética; (GTG)5; Candida; Antifungal susceptibility; PCR; Genetic characterization; (GTG)5; CNPQ::CIENCIAS DA SAUDE

…Cloreto de Trifenil tetrazolium UFAL Universidade Federal de Alagoas UFC Unidade Formadora… 

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APA (6th Edition):

Lima, G. B. C. d. (2012). Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal. (Masters Thesis). Universidade Federal de Alagoas. Retrieved from http://www.repositorio.ufal.br/handle/riufal/955

Chicago Manual of Style (16th Edition):

Lima, Gabryelle Barbosa Cordeiro de. “Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal.” 2012. Masters Thesis, Universidade Federal de Alagoas. Accessed November 28, 2020. http://www.repositorio.ufal.br/handle/riufal/955.

MLA Handbook (7th Edition):

Lima, Gabryelle Barbosa Cordeiro de. “Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal.” 2012. Web. 28 Nov 2020.

Vancouver:

Lima GBCd. Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal. [Internet] [Masters thesis]. Universidade Federal de Alagoas; 2012. [cited 2020 Nov 28]. Available from: http://www.repositorio.ufal.br/handle/riufal/955.

Council of Science Editors:

Lima GBCd. Variabilidade genética e perfil de susceptibilidade aos antifúngicos em espécies de Candida isoladas de secreção vaginal. [Masters Thesis]. Universidade Federal de Alagoas; 2012. Available from: http://www.repositorio.ufal.br/handle/riufal/955

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