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You searched for +publisher:"Universidade Estadual de Campinas" +contributor:("Serrano, Priscila de Oliveira"). One record found.

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Universidade Estadual de Campinas

1. Meloto, Carolina Beraldo, 1983-. Caracterização de uma nova isoforma da enzima COMT associada à DTM: Characterization of a new COMT isoform associated with TMD.

Degree: 2013, Universidade Estadual de Campinas

Abstract: Catecolamine-O-methyltransferase (COMT) is an enzyme with broad biological functions, including pain modulation, exerted through metabolization of substrates such as dopamine, epinephrine, and norepinephrine. It is well known that COMT activity is genetically polymorphic in humans, and correlates to individual pain perception and efficiency in its remission among temporomandibular disorder (TMD) patients treated with propranolol. Thus, our first aim was to investigate new single nucleotide polymorphism (SNP) that might mark for this benefit. Indeed, we have found a new SNP, rs165774 (G>A), that was associated with TMD or intermediate phenotypes in two different cohorts. This polymorphism, in turn, is in close proximity to a second SNP, rs165895 (T>C), both in the 3' untranslated region (3'UTR) of an alternative COMT mRNA that has not yet been characterized. Therefore, we also aimed to (i) verify the expression of this transcript in different human tissues and cell systems, tracking it through RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction); (ii) predict the crystalline structure of the enzyme encoded by it, modeling it with discrete molecular dynamics; (iii) express it in a cell system and compare its relative expression and enzymatic activity to the conventional isoform, using RT-PCR and HPLC (High Performance Liquid Chromatography), respectively; and (iv) verify the effects of SNPs rs165774 and rs165895 on the transcript, creating different mutants by site-directed mutagenesis and observing their effects on the relative expression and enzymatic activity. Our findings show that (i) the alternative COMT transcript is expressed in different human tissues and cell systems; (ii) the crystalline structure of its enzyme exhibits a unique C-terminus that is distinct from the conventional isoform; and that this transcript is (iii) less relatively expressed and its enzyme is less active than the conventional transcript and enzyme, respectively, and (iv) is regulated, at transcriptional level, by the SNPs rs165774 e rs165895. With this study, we conclude that SNP SNPs rs165774 is a strong genetic marker for TMD; that along with SNPs rs165895, they are located in the 3'UTR of an alternative COMT mRNA which proved, for the first time, to be able of encoding an alternative isoform of the enzyme that exhibits enzymatic activity; and that this alternative COMT mRNA is regulated, at transcriptional level, by both SNPs Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Rizzatti-Barbosa, Celia Marisa, 1957- (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Clínica Odontológica (nameofprogram), Camparis, Cinara Maria (committee member), Serrano, Priscila de Oliveira (committee member), Del Bel Cury, Altair Antoninha (committee member), Line, Sergio Roberto Peres (committee member).

Subjects/Keywords: Transtornos da articulação temporomandibular; Polimorfismo de nucleotídeo único; RNA mensageiro; Temporomandibular joint disorders; Polymorphism single nucleotide; RNA messenger

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APA (6th Edition):

Meloto, Carolina Beraldo, 1. (2013). Caracterização de uma nova isoforma da enzima COMT associada à DTM: Characterization of a new COMT isoform associated with TMD. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/290517

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Meloto, Carolina Beraldo, 1983-. “Caracterização de uma nova isoforma da enzima COMT associada à DTM: Characterization of a new COMT isoform associated with TMD.” 2013. Thesis, Universidade Estadual de Campinas. Accessed January 23, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290517.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Meloto, Carolina Beraldo, 1983-. “Caracterização de uma nova isoforma da enzima COMT associada à DTM: Characterization of a new COMT isoform associated with TMD.” 2013. Web. 23 Jan 2021.

Vancouver:

Meloto, Carolina Beraldo 1. Caracterização de uma nova isoforma da enzima COMT associada à DTM: Characterization of a new COMT isoform associated with TMD. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2021 Jan 23]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/290517.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meloto, Carolina Beraldo 1. Caracterização de uma nova isoforma da enzima COMT associada à DTM: Characterization of a new COMT isoform associated with TMD. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/290517

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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