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You searched for +publisher:"Universidade Estadual de Campinas" +contributor:("Pereira, Graziela Hanna"). One record found.

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Universidade Estadual de Campinas

1. Kuboyama, Rogerio Hakio. Detecção de cepas de Klebsiella pneumoniea produtoras de beta-lactamases de espectro estendido em pacientes assistidos em hospitais terciarios na cidade de Campinas : epidemiologia molecular e fatores de risco: Detection of extended-spectrum-beta-lactamase-producing strains of Klebsiella pneumoniea isolated from patients hospitalized in tertiary-care hospitals in Campinas : molecular epidemiology and risk factors.

Degree: 2009, Universidade Estadual de Campinas

Abstract: The objectives of this study conducted restrospectively using strains isolated from clinical specimens obtained from patients hospitalized in two Brazilian hospitals between February 2001 to June 2004 were to describe the presence of extended-spectrum b-lactamase (ESBL)-producing Klebsiella pneumoniae strains, the best method and the preferred substrate for screening and confirming ESBL production and the epidemiological relatedness of ESBL-producing strains and analyse the risk factors for infection due to ESBL-producing K. pneumoniae. To investigate the genetic relatedness of the strains, plasmid analysis and chromosomal DNA analysis by pulsed-field gel electrophoresis were used. A total of 89 K. pneumoniae were collected from diverse body sites. The most commom specimens yielding ESBL-producing strains were urine (12.4%) and blood (10.1%). Using CLSI criteria (ESBL screening breakpoints), 35 K. pneumoniae (39.3%) had presumptive ESBL phenotype, while using the double-disk approximation test (DDAT) characteristic clavulanate-induced distortions of inhibition zones were found in 96, 62.5, 50, 18.8 and 12.5% of the strains around the disks containing aztreonam, cefotaxime, ceftazidime, ceftriaxone and cefpodoxime, respectively. Of 89 isolates, 32 (36%) produced ESBL based on the confirmatory Oxoid combination disk method. The disk containing cefotaxime was able to confirm 100% of the ESBL producers while the disk containing ceftazidime was not able to confirm 2 ESBL-positive strains. Ten and 32 different plasmid profiles were observed among the ESBL-producing K. pneumoniae and non ESBL producers, respectively. Pulsed-field gel electrophoresis showed the best discriminatory power giving 15 and 55 different chromosomal DNA profiles among the ESBL-positive and ESBL-negative K. pneumoniae, respectively. From univariate analysis, variables significantly associated with infection by an ESBL-producing strain of K. pneumoniae included the following: use of 4st-generation cephalosporins, lincosamide, carbapenems, glycopeptides, recent surgery, tracheostomy, age, days in using of lincosamide, days in using of glycopeptides, total number of antibiotics and duration of the antimicrobial therapy. The only variable that remained independent risk factor for acquiring infection due to ESBL-producing K. pneumoniae after multivariable analysis using a logistic regression model, which included the variables associated with acquiring infection by ESBL-producing K. pneumoniae by univariate analysis (P < 0.05), was total number of antibiotics (OR, 1.60; 95%CI, 1.194-2.145; P = 0.0017). In summary, these data indicate that ESBL-producing K. pneumoniae occur at a relatively high incidence at our institutions and the plasmid analysis is not sufficient to identify relationships between ESBL-producing strains of K. pneumoniae. The ESBL screening breakpoints and DDAT with aztreonam appear to be good indicators in presumptive detection of ESBL-producing strains and the cefotaxime used in the Oxoid combination disk method constituted… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Moretti, Maria Luiza, 1953- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Clínica Médica (nameofprogram), Yasuda, Maria Aparecida Shikanai (committee member), Pereira, Graziela Hanna (committee member), Aoki, Francisco Hideo (committee member), Yano, Tomomasa (committee member).

Subjects/Keywords: Klebsiella pneumoniae; Eletroforese em gel de campo pulsado; Plasmideos; Fatores de risco; Epidemiologia molecular; Infecção hospitalar; Beta-lactamas; Klebsiella pneumoniae; Electrophoresis, gel, pulsed-field; Plasmids; Risk factors; Molecular epidemiology; Nosocomial infections; Beta-lactamases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kuboyama, R. H. (2009). Detecção de cepas de Klebsiella pneumoniea produtoras de beta-lactamases de espectro estendido em pacientes assistidos em hospitais terciarios na cidade de Campinas : epidemiologia molecular e fatores de risco: Detection of extended-spectrum-beta-lactamase-producing strains of Klebsiella pneumoniea isolated from patients hospitalized in tertiary-care hospitals in Campinas : molecular epidemiology and risk factors. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/311054

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Kuboyama, Rogerio Hakio. “Detecção de cepas de Klebsiella pneumoniea produtoras de beta-lactamases de espectro estendido em pacientes assistidos em hospitais terciarios na cidade de Campinas : epidemiologia molecular e fatores de risco: Detection of extended-spectrum-beta-lactamase-producing strains of Klebsiella pneumoniea isolated from patients hospitalized in tertiary-care hospitals in Campinas : molecular epidemiology and risk factors.” 2009. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311054.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Kuboyama, Rogerio Hakio. “Detecção de cepas de Klebsiella pneumoniea produtoras de beta-lactamases de espectro estendido em pacientes assistidos em hospitais terciarios na cidade de Campinas : epidemiologia molecular e fatores de risco: Detection of extended-spectrum-beta-lactamase-producing strains of Klebsiella pneumoniea isolated from patients hospitalized in tertiary-care hospitals in Campinas : molecular epidemiology and risk factors.” 2009. Web. 25 Feb 2021.

Vancouver:

Kuboyama RH. Detecção de cepas de Klebsiella pneumoniea produtoras de beta-lactamases de espectro estendido em pacientes assistidos em hospitais terciarios na cidade de Campinas : epidemiologia molecular e fatores de risco: Detection of extended-spectrum-beta-lactamase-producing strains of Klebsiella pneumoniea isolated from patients hospitalized in tertiary-care hospitals in Campinas : molecular epidemiology and risk factors. [Internet] [Thesis]. Universidade Estadual de Campinas; 2009. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311054.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuboyama RH. Detecção de cepas de Klebsiella pneumoniea produtoras de beta-lactamases de espectro estendido em pacientes assistidos em hospitais terciarios na cidade de Campinas : epidemiologia molecular e fatores de risco: Detection of extended-spectrum-beta-lactamase-producing strains of Klebsiella pneumoniea isolated from patients hospitalized in tertiary-care hospitals in Campinas : molecular epidemiology and risk factors. [Thesis]. Universidade Estadual de Campinas; 2009. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311054

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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