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You searched for +publisher:"Universidade Estadual de Campinas" +contributor:("Mendes, Rodrigo"). Showing records 1 – 2 of 2 total matches.

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Universidade Estadual de Campinas

1. Noronha, Melline Fontes, 1985-. Metagenômica comparativa de comunidades microbianas de solos de biomas globais: Comparative metagenomics of microbial communities from global biome soils.

Degree: 2016, Universidade Estadual de Campinas

Abstract: Biome is a geographical unit characterized according to its vegetation type, macro-climate, soil, and specific altitude. In contrast, a microbiome is the totality of microorganisms and their collective genetic material present in a given environment. Although soil microbial communities have shown to vary across many spatial scales, soils between ecosystems showed to be driven by some biogeographical trends. In order to investigate taxonomic and functional convergence within soils from the same biome type, thirty publically-available metagenomes from 11 globally distributed biomes were selected and clustered by biome groups (i.e. forest, grassland, tundra, semiarid and desert) based on vegetation features. Functional analyses revealed a close pattern among biome groups, in which protein biosynthesis, central carbohydrate metabolism, and antibiotic resistance were the most statistically different metabolisms annotated by SEED subsystems. In order to provide a better analytical resolution of those metabolisms, metagenomic reads were annotated using the Carbohydrate-Active enZYmes database (Cazy), Antibiotic Resistance gene DataBase (ARDB) and, additionally, the Heat Shock Protein Information Resource (HSPIR). Carbohydrate-active enzyme analyses showed that biomass degradation, sucrose and starch metabolism, cell wall biosynthesis and alginate degradation were overrepresented in forest and grasslands soils. As expected, desiccation and other stress resistance genes were more abundant in deserts and semiarid soils. Antibiotic Resistance Genes (ARGs) were prevalent in forest and grassland soils, where multidrug efflux pumps were the most abundant ARG class, with the majority of the reads assigned to Proteobacteria. Heat Shock Proteins (HSPs) were more abundant in tundra, semiarid and desert soils. Although HSP70 and HSP100 were uniformly distributed across biomes, HSP90 were overrepresented in forest and grassland soils when compared to others biomes groups. HSP60 and HSP20, which are predominantly from Archaea, were more abundant in the saline desert soils. Our results suggest that local environmental conditions select for the enrichment of specific functions important for microbial survival in those ecosystems Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Mendes, Rodrigo (committee member), Taketani, Rodrigo Gouvêa (committee member), Cabral, Lucélia (committee member), Carazzolle, Marcelo Falsarella (committee member).

Subjects/Keywords: Metagenômica; Solos; Ecossistema; Carboidratos; Drogas - Resistência em micro-organismos; Metagenomics; Soils; Ecosystem; Carbohydrates; Drug resistance in microorganisms

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Noronha, Melline Fontes, 1. (2016). Metagenômica comparativa de comunidades microbianas de solos de biomas globais: Comparative metagenomics of microbial communities from global biome soils. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/331181

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Noronha, Melline Fontes, 1985-. “Metagenômica comparativa de comunidades microbianas de solos de biomas globais: Comparative metagenomics of microbial communities from global biome soils.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/331181.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Noronha, Melline Fontes, 1985-. “Metagenômica comparativa de comunidades microbianas de solos de biomas globais: Comparative metagenomics of microbial communities from global biome soils.” 2016. Web. 16 Jan 2021.

Vancouver:

Noronha, Melline Fontes 1. Metagenômica comparativa de comunidades microbianas de solos de biomas globais: Comparative metagenomics of microbial communities from global biome soils. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331181.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Noronha, Melline Fontes 1. Metagenômica comparativa de comunidades microbianas de solos de biomas globais: Comparative metagenomics of microbial communities from global biome soils. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331181

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Universidade Estadual de Campinas

2. Lacerda Júnior, Gileno Vieira, 1987-. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.

Degree: 2017, Universidade Estadual de Campinas

Abstract: Caatinga is an exclusively Brazilian biome, poorly studied and preserved, with an exuberant and exclusive biological diversity. This ecosystem consists of a dry tropical forest adapted to severe climatic conditions, including high temperatures and solar irradiation, soil acidity, and water deficit caused by the seasonal rainfall regime. Therefore, it is expected to harbor a soil microbiota carrying unique and versatile enzymatic arsenals with a potential biotechnological application. Resident soil microorganisms are capable of decomposing lignin (second most abundant biopolymer of the biosphere) using a set of ligninolytic enzymes which can also be applied in the treatment of effluents, pulp and paper industry, biofuels productions, among other industrial processes. Considering that about 99% of soil microorganisms cannot be isolated by conventional culture methods, molecular approaches based on the direct extraction of environmental genetic material, such as "metagenomics", are needed to provide more precise information about the diversity and biotechnological potential of the native microbial community. Thus, this study uses an integrated approach of functional (based on biological activity and sequence similarity) and descriptive metagenomics, with the aim of identifying new lignocellulolytic enzymes, in addition to analyzing the impact of seasonality and agricultural practices (irrigation/fertilization) in the community structure and functional responses of the Caatinga soil microbiome. The functional screening based on the phenotypic expression of 40,000 metagenomic clones did not show any ligninolytic activity. However, the molecular screening by PCR allowed the identification of a bacterial Laccase-like Multicopper oxidase, affiliated with the genus Mesorhizobium. The large-scale sequencing of a soil metagenomic library and subsequent in silico analysis revealed a large enzymatic repertoire related to the lignocellulosic degradation carried by different bacterial groups with potential application in the biofuels production as well as other environmental processes. In an ecological approach, it was observed the enrichment of the phyla Actinobacteria and Proteobacteria in the drought and rainy seasonal period, respectively. The data suggest that microorganisms thriving on native soils of the dry period are specialized in monosaccharides and disaccharides utilization, as well as osmoprotective sugars such as trehalose. In contrast, the soil microbiome affected by land use was mainly driven by chemical factors, with the enrichment of the Acidobacteria phylum and genes related to nitrification and denitrification processes, and to oxidative stress. These results suggest that the nutrients input and the breakdown of the natural cycles of the water regime may impact the biogeochemical cycles, affecting the natural functionality of the Caatinga semiarid soils Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Souza, Anete Pereira de (committee member), Vicentini, Renato (committee member), Mendes, Rodrigo (committee member), Andreote, Fernando Dini (committee member).

Subjects/Keywords: Caatinga; Micro-organismos do solo; Metagenômica; Bioprospecção; Enzimas ligninolíticas; Caatinga; Soil microbiology; Metagenomics; Bioprospecting; Ligninolytic enzymes

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lacerda Júnior, Gileno Vieira, 1. (2017). Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Lacerda Júnior, Gileno Vieira, 1987-. “Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.” 2017. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Lacerda Júnior, Gileno Vieira, 1987-. “Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.” 2017. Web. 16 Jan 2021.

Vancouver:

Lacerda Júnior, Gileno Vieira 1. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. [Internet] [Thesis]. Universidade Estadual de Campinas; 2017. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lacerda Júnior, Gileno Vieira 1. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. [Thesis]. Universidade Estadual de Campinas; 2017. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.