+publisher:"Universidade Estadual de Campinas" +contributor:("Hyslo, Stephen")

Universidade Estadual de Campinas

1. Rui, Edmilson. Mutagenese da proteina HBx do virus da hepatite B e estudo da interação com RNA e proteinas humanas: Mutagenesis of hepatitis B virus protein HBx and studies of its interaction with RNA and human proteins.

Degree: 2005, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314356

► Abstract: Viral hepatitis is an important global public health problem. Epidemiological data show that worldwide 350 million people are chronically infected with the hepatitis B… (more)

▼ Abstract: Viral hepatitis is an important global public health problem. Epidemiological data show that worldwide 350 million people are chronically infected with the hepatitis B virus. About 95% of the infections cure spontaneously, but the chronically infected patients may develop liver cirrhosis or even hepatocellular carcinoma (HCC). A large body of evidence points to the viral onco-protein HBx as the principal cause for the cellular transformation. The majority of studies on HBx function suggest that it is a regulatory protein with pleiotropic functions and its capacity to induce tumor growth may be due to its ability to interact with a diverse array of cellular proteins. In the present study we investigated molecular and structural aspects of the function of the HBx protein in order to be able to shed light on the process of cellular transformation. We were able to demonstrate that HBx protein has the ability to bind to an AU-rich RNA sequences present in the mRNAs of certain proto-oncogenes such as c-myc and c-fos. The generation of truncated proteins allowed to map the region of interaction of HBx with the RNA. Furthermore, we performed site directed mutagenesis studies of HBx protein and substituted all of its cysteine residues with serines. Our data suggest that the cysteine residues in the HBx protein are of minor importance for its interaction with RNA, p53 and RXR proteins. Finally, we discovered in E4F a new human interacting protein partner for the HBx protein. The transcription factor E4F has been functionally implicated in the control of the cell cycle, the chromosomal segregation and embriogenesis. In studies using the yeast we further showed that wild-type HBx, as well as its mutated forms, can physically interact with the E4F protein and regulate its function. This indicates a possible new mechanism of cellular transformation and the regulation of the viral transcription, since the human protein E4F demonstrated the capacity to bind to the enhancer II region of the HBV genome Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Kobarg, Jörg, 1965- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Costa, Fabio Trindade Maranhão (committee member), Benedetti, Celso Eduardo (committee member), Hyslo, Stephen (committee member), Marangoni, Sérgio (committee member).

Subjects/Keywords: Oncogeneses; Fígado - Câncer; Ativação viral; Oncogeneses; Liver- Cancer; Virus activation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rui, E. (2005). Mutagenese da proteina HBx do virus da hepatite B e estudo da interação com RNA e proteinas humanas: Mutagenesis of hepatitis B virus protein HBx and studies of its interaction with RNA and human proteins. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314356

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rui, Edmilson. “Mutagenese da proteina HBx do virus da hepatite B e estudo da interação com RNA e proteinas humanas: Mutagenesis of hepatitis B virus protein HBx and studies of its interaction with RNA and human proteins.” 2005. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314356.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rui, Edmilson. “Mutagenese da proteina HBx do virus da hepatite B e estudo da interação com RNA e proteinas humanas: Mutagenesis of hepatitis B virus protein HBx and studies of its interaction with RNA and human proteins.” 2005. Web. 28 Feb 2021.

Vancouver:

Rui E. Mutagenese da proteina HBx do virus da hepatite B e estudo da interação com RNA e proteinas humanas: Mutagenesis of hepatitis B virus protein HBx and studies of its interaction with RNA and human proteins. [Internet] [Thesis]. Universidade Estadual de Campinas; 2005. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314356.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rui E. Mutagenese da proteina HBx do virus da hepatite B e estudo da interação com RNA e proteinas humanas: Mutagenesis of hepatitis B virus protein HBx and studies of its interaction with RNA and human proteins. [Thesis]. Universidade Estadual de Campinas; 2005. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314356

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Freire, Ana Claudia Galvão. Papel de fosfatases na viabilidade de celulas da leucemia mieloide humana tratadas com diterpeno lactona.

Degree: 2002, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314032

► Abstract: patient with acute myeloid leukemia. This culture proliferates continuously in suspension and predominantly consists of promyelocytes and has been used as tool for cytotoxic… (more)

▼ Abstract: patient with acute myeloid leukemia. This culture proliferates continuously in suspension and predominantly consists of promyelocytes and has been used as tool for cytotoxic studies of drugs, differentiation and cell death. ln this work was evaluated the cytotoxicity of protein phosphatases inhibitors (okadaic acid and pervanadate) and dehydrocrotonin (tDCTN) through following viability parameters, after 24h of the cells treatment: MTT reduction (mitochondrial integrity), protein determination (cell number indication) and fosfatase activity (cell metabolism). Both inhibitors presented cytotoxic effect on HL60 cells. In relation to mitochondrial function and phosphatase activity, these cells presented only 20% of viability in presence of okadaic acid (100 nM) and pervanadate (200 1lM). For t-DCTN were obtained the following IC50 values: 500 and 300 mM for protein content and MTT reduction, respectively. Other approached aspect was the relation to cellular redox state with a possible differentiation and cell death induction. For this purpose were determined: NBT reduction, DNA fragmentation, the loss of plasma membrane asymmetry and total GSH. t-DCTN presented low capacity of differentiation induction (20%). However, in the presence of protein phosphatase inhibitors this effect was not observed. Apoptosis was induced when the cells were treated with okadaic acid (34%) and plus t-DCTN this effect was increased (65,28%). According to the results obtained, we can suggest the importance of chemical modulation of protein phosphatases in several cellular processes, since cytotoxic effects presented by these compounds could be explained by redox state changing and/or direct effect (adducts formation) on these enzymes or other macromolecules Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Ferreira, Carmen Veríssima, 1969- (advisor), Haun Quiros, Nora Marcela, 1948- (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Duran Caballero, Nelson Eduardo (committee member), Hyslo, Stephen (committee member), Novello, Jose Camillo (committee member).

Subjects/Keywords: Fosfatases; Leucemia; Apoptose

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Freire, A. C. G. (2002). Papel de fosfatases na viabilidade de celulas da leucemia mieloide humana tratadas com diterpeno lactona. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314032

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freire, Ana Claudia Galvão. “Papel de fosfatases na viabilidade de celulas da leucemia mieloide humana tratadas com diterpeno lactona.” 2002. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314032.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freire, Ana Claudia Galvão. “Papel de fosfatases na viabilidade de celulas da leucemia mieloide humana tratadas com diterpeno lactona.” 2002. Web. 28 Feb 2021.

Vancouver:

Freire ACG. Papel de fosfatases na viabilidade de celulas da leucemia mieloide humana tratadas com diterpeno lactona. [Internet] [Thesis]. Universidade Estadual de Campinas; 2002. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314032.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freire ACG. Papel de fosfatases na viabilidade de celulas da leucemia mieloide humana tratadas com diterpeno lactona. [Thesis]. Universidade Estadual de Campinas; 2002. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314032

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

3. Dieamant, Gustavo de Campos. Resposta neuro-imuno-endocrinologica da pele relacionada ao fotoenvelhecimento : avaliação in vitro e clinica de um novo composto como alternativa terapeutica em dermatologia: Skin neuro-immuno-endocrinology response related to photoaging : in vitro and clinical assessment of a new compound as therapeutical alternative in dermatology.

Degree: 2008, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311279

► Abstract: Cutaneous ageing is a complex biological phenomenon consisting of two components; intrinsic ageing, which is largely genetically determined and extrinsic ageing caused by environmental… (more)

▼ Abstract: Cutaneous ageing is a complex biological phenomenon consisting of two components; intrinsic ageing, which is largely genetically determined and extrinsic ageing caused by environmental exposure, primarily UV light. Under the impact of ultraviolet radiation (UVR), skin undergoes several changes, including enhanced pigmentation, photodamage and immunosuppression. Because photoaging culminates in important alterations in skin function and appearance, we can separate this work in two different phases. In the first phase, we evaluated the effects of ultraviolet radiation (UVR) on skin neuro-immuneendocrinological response through the measurement of specific parameters in fibroblasts and keratinocytes human cell cultures. After the conclusion of experiments proposed for the first phase of this work, the possible in vitro and in vivo effects of Rhodiola rosea extract (ERR) in combination to di-peptide Lcarnosine (LC) in the control of deleterious effects of UVR exposure on the skin were evaluated. Our results demonstrated that during the UVR, the cell cultures promoted crucial alterations in the parameters evaluated, mainly after chronic exposure. The alterations observed were: the reduction in the production of IL-1a, TNF-a, IFN-g, b-endorphin and enkephalin, reduction in the levels of matrix metalloproteinase tissue inhibitor-1, increase of metalloproteinase activity, reduction of collagen and elastin production and inhibition of the antioxidant enzymes superoxide dismutase and catalase. In the cell groups that received ERR, we observed an important adaptogenic function of the extract, because in the majority of the parameters, it was able to modulate the cell response in a dose-dependent manner. The results obtained in the LC treated groups, we could also observe important effects, mainly in collagen and elastin synthesis and in the antioxidant enzymes activities. When we consider the response obtained with the association of both ERR and LC (ARRLC), we could see that it is not able to enhance the effects obtained with ERR or LC separately. Therefore, due to the complexity of the neuro-immune-endocrinological response during the photoaging, we can conclude that the use of compounds with different mechanisms of action is crucial, because distinct metabolic process lead to tissue damage and terrible appearance of photodamaged skin. For the clinical trials, a placebo emulsion or a emulsion containing 1% of ARRLC were used by the volunteers in two independent studies, in which the first one was performed during 28 days for evaluation of subjective parameters, including skin comfort, reduction of skin dryness sensation and reduction of skin discomfort sensation after Stinging-Test (ST). The second study was performed during 56-days and the evaluations were carried out on D1, D28 and D56. The parameters evaluated were the improvement of cutaneous relief and the reduction of transepidermal water loss. The obtained results demonstrated that approximately 90% of the volunteers perceived an increase in skin comfort and… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Queiroz, Mary Luci de Souza, 1948- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Farmacologia (nameofprogram), Colombi, Debora (committee member), Araújo, Albetiza Lôbo de (committee member), Stasi, Luiz Claudio di (committee member), Hyslo, Stephen (committee member).

Subjects/Keywords: Radiação ultravioleta; Endorfinas; Citocinas; Cosméticos; Envelhecimento; Ultraviolet radiation; Endorphins; Cytokines; Cosmetics; Aging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dieamant, G. d. C. (2008). Resposta neuro-imuno-endocrinologica da pele relacionada ao fotoenvelhecimento : avaliação in vitro e clinica de um novo composto como alternativa terapeutica em dermatologia: Skin neuro-immuno-endocrinology response related to photoaging : in vitro and clinical assessment of a new compound as therapeutical alternative in dermatology. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/311279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dieamant, Gustavo de Campos. “Resposta neuro-imuno-endocrinologica da pele relacionada ao fotoenvelhecimento : avaliação in vitro e clinica de um novo composto como alternativa terapeutica em dermatologia: Skin neuro-immuno-endocrinology response related to photoaging : in vitro and clinical assessment of a new compound as therapeutical alternative in dermatology.” 2008. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dieamant, Gustavo de Campos. “Resposta neuro-imuno-endocrinologica da pele relacionada ao fotoenvelhecimento : avaliação in vitro e clinica de um novo composto como alternativa terapeutica em dermatologia: Skin neuro-immuno-endocrinology response related to photoaging : in vitro and clinical assessment of a new compound as therapeutical alternative in dermatology.” 2008. Web. 28 Feb 2021.

Vancouver:

Dieamant GdC. Resposta neuro-imuno-endocrinologica da pele relacionada ao fotoenvelhecimento : avaliação in vitro e clinica de um novo composto como alternativa terapeutica em dermatologia: Skin neuro-immuno-endocrinology response related to photoaging : in vitro and clinical assessment of a new compound as therapeutical alternative in dermatology. [Internet] [Thesis]. Universidade Estadual de Campinas; 2008. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dieamant GdC. Resposta neuro-imuno-endocrinologica da pele relacionada ao fotoenvelhecimento : avaliação in vitro e clinica de um novo composto como alternativa terapeutica em dermatologia: Skin neuro-immuno-endocrinology response related to photoaging : in vitro and clinical assessment of a new compound as therapeutical alternative in dermatology. [Thesis]. Universidade Estadual de Campinas; 2008. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

4. Amazonas, Roberto Bleuel. Alterações renais precoces em um modelo experimental que combina hipertensão arterial genetica e diabetes mellitus : efeitos do tratamento anti-hipertensivo: Early renal abnormalities in a experimental model that combind genetic arterial hypertension and diabetes mellitus : effect of anty-hypertensive treatment.

Degree: 2006, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309966

► Abstract: In diabetic patients hypertension is the main secondary factor associated with the development and progression of renal disease. However, the mechanism of interaction between… (more)

▼ Abstract: In diabetic patients hypertension is the main secondary factor associated with the development and progression of renal disease. However, the mechanism of interaction between diabetes and hypertension to exacerbate diabetic renal disease is poorly understood. The aim of this study was to evaluate the effect of presence of hypertension had on nephrin expression, glomerular hypertrophy, renal celI replication and accumulation of glomerular TGF f3 and fibronectin in a model of genetic hypertension and experimental diabetes mellitus. Four-week-old spontaneously hypertensive rats (SHR) and their normotensive control Wistar K yoto (WKY) were rendered diabetic by means of intravenously injection of streptozotocin. To further assess the contribution ofhypertension to renal abnormalities diabetic SHR were randomÍzed for no treatment, or for treatment with captopril, losartan or triple therapy (hydrochlorothiazide, reserpine and hydralazine) for 20 days. Increase in systolic blood pressure was equally prevented by captopril (113 :i: 9mmHg), losartan (115 :!:: 16) and triple therapy (108 :i: 12, p<0.005). Glomerular expression of nephrin was reduced in SHR and further diminished in diabetic SHR in comparison with controls and the antihypertensive treatment prevents the reduction in glomerular expression of nephrin. Glomerular sÍze was higher (p<0.005) in diabetic SHR (27,300 :i: 2130 Jim2) compared with non-diabetic SHR (23,800 :!:: 307). The antihypertensive therapy with captopril (23,900 :i: 175), losartan (23,800 :i:120), and triple therapy (23,400 :i:21O) prevented the glomerular enlargement in diabetic SHR. Glomerular expression of TGF f3 and fibronectin was significantly increased in diabetic SHR as compared to the controls, and was prevented with captopril, losartan and triple therapy. The number of replicating glomerular cell significantly decreased in diabetic SHR and it was restored by alI three antihypertensive regimes. The glomerular expression of p27Kipl was significantly higher in diabetic SHR but it was not modified by antihypertensive treatment. In diabetic rats the presence of genetic hypertension induced early renal abnormalities that were prevented by strict control of blood pressure. These abnormalities may be involved in the mechanism of interaction between diabetes and hypertension to exacerba te renal disease Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Faria, Jose Butori Lopes de, 1955- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Clínica Médica (nameofprogram), Santos, Bento Fortunato Cardoso dos (committee member), Figueiredo, Carlos Eduardo Poli de (committee member), Hyslo, Stephen (committee member), Franchini, Kleber Gomes (committee member).

Subjects/Keywords: Diabetes Mellitus; Nefropatias diabeticas; Hipertensão; Inibidores da enzima conversora da angiotensina; Diabetic nephropathy; Arterial hypertension; Angiotensin converting enzyme - Inhibitors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Amazonas, R. B. (2006). Alterações renais precoces em um modelo experimental que combina hipertensão arterial genetica e diabetes mellitus : efeitos do tratamento anti-hipertensivo: Early renal abnormalities in a experimental model that combind genetic arterial hypertension and diabetes mellitus : effect of anty-hypertensive treatment. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/309966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amazonas, Roberto Bleuel. “Alterações renais precoces em um modelo experimental que combina hipertensão arterial genetica e diabetes mellitus : efeitos do tratamento anti-hipertensivo: Early renal abnormalities in a experimental model that combind genetic arterial hypertension and diabetes mellitus : effect of anty-hypertensive treatment.” 2006. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/309966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amazonas, Roberto Bleuel. “Alterações renais precoces em um modelo experimental que combina hipertensão arterial genetica e diabetes mellitus : efeitos do tratamento anti-hipertensivo: Early renal abnormalities in a experimental model that combind genetic arterial hypertension and diabetes mellitus : effect of anty-hypertensive treatment.” 2006. Web. 28 Feb 2021.

Vancouver:

Amazonas RB. Alterações renais precoces em um modelo experimental que combina hipertensão arterial genetica e diabetes mellitus : efeitos do tratamento anti-hipertensivo: Early renal abnormalities in a experimental model that combind genetic arterial hypertension and diabetes mellitus : effect of anty-hypertensive treatment. [Internet] [Thesis]. Universidade Estadual de Campinas; 2006. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amazonas RB. Alterações renais precoces em um modelo experimental que combina hipertensão arterial genetica e diabetes mellitus : efeitos do tratamento anti-hipertensivo: Early renal abnormalities in a experimental model that combind genetic arterial hypertension and diabetes mellitus : effect of anty-hypertensive treatment. [Thesis]. Universidade Estadual de Campinas; 2006. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

5. Lorenzetti, Raquel. Metodo de quantificação de nucleotideos por HPLC-MS/MS e avaliação da atividade de analogos de sildenafil sobre fosfodiesterase: Metho of quantification of nucleotides by HPLC-MS/MS and evaluation of the activity of sildenafil analoges in phosphodiesterase.

Degree: 2007, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309493

► Abstract: In the present work a new method for the dosage of the activity of phosphodiesterase was standardized in vitro, for HPLC-MS/MS. This new method… (more)

▼ Abstract: In the present work a new method for the dosage of the activity of phosphodiesterase was standardized in vitro, for HPLC-MS/MS. This new method obtained to present exactness, precision, sensitivity, and rapidity in the analyses; monitoring the nucleotides (AMP, GMP, cAMP and cGMP). The development of new drug derived from an archetype points with respect to the molecule attainment with one better pharmacokinetic profile or one better relation structure-activity. Currently the sildenafil is considered the main drug for the treatment of erectile dysfunction. In this work, we evaluate new analogous called composites of sildenafil (carbonate of lodenafil, dimer urea and dimer uretana). The analogous ones had been analyzed how much the activity in PDE5 and platelet aggregation human being, in vitro. The stability of these composites was determined, in human acid way and plasma, in vitro, beyond its possible metabolites in microsomes and hepatocytes of rat in vitro, and its pharmacokinetic profile after intravenous and oral, in dog, in vivo. The results had shown that the analogous of sildenafil inhibit the activity of PDE and they do not inhibit the platelet aggregation in a similar way that the sildenafil in vitro, however potencializam the action of the giver of NO (SNP). The analogous ones of sildenafil are presented steady in human acid way and plasma. In the metabolization assay, metabolization of dimer urea and dimer uretana was not observed, however the lodenafil carbonate was metabolizado mainly in lodenafil, in vitro. The lodenafil carbonate quickly is biotransformation in lodenafil, after administration v.i. and v.o. in dog. We conclude that this work presents a new method for analyze activity of PDEs and a new therapeutically perspective for the erectile dysfunction, represented for lodenafil carbonate, which inhibits concentration-dependent the activity of PDE5 Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), De Nucci, Gilberto, 1958- (advisor), Nucci, Gilberto de, 1958- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Farmacologia (nameofprogram), Pereira, Darcio Gomes (committee member), Almeida, Eros Antonio de (committee member), Hyslo, Stephen (committee member), Lacchini, Silvia (committee member).

Subjects/Keywords: Inibidores de fosfodiesterase; Nucleotideos; Espectrometria de massas; Óxido nítrico; Nucleotideos ciclicos - Técnica; Phosphodiesterase inhibitor; Nucleotides; Mass spectrometry; Nitric oxide; Ciclic nucleotides technique

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lorenzetti, R. (2007). Metodo de quantificação de nucleotideos por HPLC-MS/MS e avaliação da atividade de analogos de sildenafil sobre fosfodiesterase: Metho of quantification of nucleotides by HPLC-MS/MS and evaluation of the activity of sildenafil analoges in phosphodiesterase. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/309493

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lorenzetti, Raquel. “Metodo de quantificação de nucleotideos por HPLC-MS/MS e avaliação da atividade de analogos de sildenafil sobre fosfodiesterase: Metho of quantification of nucleotides by HPLC-MS/MS and evaluation of the activity of sildenafil analoges in phosphodiesterase.” 2007. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/309493.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lorenzetti, Raquel. “Metodo de quantificação de nucleotideos por HPLC-MS/MS e avaliação da atividade de analogos de sildenafil sobre fosfodiesterase: Metho of quantification of nucleotides by HPLC-MS/MS and evaluation of the activity of sildenafil analoges in phosphodiesterase.” 2007. Web. 28 Feb 2021.

Vancouver:

Lorenzetti R. Metodo de quantificação de nucleotideos por HPLC-MS/MS e avaliação da atividade de analogos de sildenafil sobre fosfodiesterase: Metho of quantification of nucleotides by HPLC-MS/MS and evaluation of the activity of sildenafil analoges in phosphodiesterase. [Internet] [Thesis]. Universidade Estadual de Campinas; 2007. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309493.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lorenzetti R. Metodo de quantificação de nucleotideos por HPLC-MS/MS e avaliação da atividade de analogos de sildenafil sobre fosfodiesterase: Metho of quantification of nucleotides by HPLC-MS/MS and evaluation of the activity of sildenafil analoges in phosphodiesterase. [Thesis]. Universidade Estadual de Campinas; 2007. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309493

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

6. Cavagis, Alexandre Donizeti Martins. Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação.

Degree: 2005, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314033

► Abstract: The principal aim of this work was to assess the MAPKs family status in human leukaemia cells induced to undergo apoptosis and differentiation. In… (more)

▼ Abstract: The principal aim of this work was to assess the MAPKs family status in human leukaemia cells induced to undergo apoptosis and differentiation. In chapter one, the effect of tetrahydroxyquinone (THQ), a highly redox active molecule which can participate of a redox cycle with semiquinone radicals leading to the formation of reactive oxygen species (ROS), was investigated in HL60 cells. THQ caused substantial ROS formation followed by cytotoxicity that was sensitive to glutathione and N-acetyl-L-cysteine. Furthermore, ROS production coincided with HL60 cell apoptosis through the mitochondrial pathway and was followed by reduced activity of various anti-apoptotic survival molecules, including the protein kinase B pathway. Importantly, transfection of protein kinase B into HL60 cells leading to an artificial increase in protein kinase B activity inhibited ROS-dependent cytotoxicity. In chapter two, peptide arrays of 1176 different kinase consensus substrates unambiguously identified the MAP kinase pathway as a major target for violacein, the anti-leukemic purple-colored pigment produced by Chromobacterium violaceum from the Amazon River that stimulates HL60 cells to differentiate into monocytes and granulocytes. The results also indicate that kinome profiling using metabolic arrays is a promising and suitable tool for studying the molecular mechanisms of drug action. Chapter three is a review article in Portuguese about riboflavin, a component of the vitamin B2 complex, that has important roles in biochemistry, especially in redox reactions due to its ability to participate in both one- and two-electron transfers as well as acting as photosensitizer. This article describes the peculiar and multifunctional behavior of riboflavin which allows it to take part in several biochemical pathways both as nucleophile and electrophile. Moreover, the association of this vitamin with different diseases, including cancer and cardiovascular diseases, is also discussed. Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Ferreira, Carmen Veríssima, 1969- (advisor), Aoyama, Hiroshi, 1943- (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Freire, Ana Claudia Galvão (committee member), Alves, Armindo Antonio (committee member), Buzalaf, Marilia Afonso Rabelo (committee member), Hyslo, Stephen (committee member).

Subjects/Keywords: MAPKs; Apoptose; Leucemia; Celulas HL60; Tetrahidroxiquinona; Violaceina; Diferenciação celular; MAPKs; Apoptosis; Leukaemia; HL60 cells; Tetrahydroxyquinone; Violacein; Cells - Differentiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cavagis, A. D. M. (2005). Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314033

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cavagis, Alexandre Donizeti Martins. “Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação.” 2005. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314033.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cavagis, Alexandre Donizeti Martins. “Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação.” 2005. Web. 28 Feb 2021.

Vancouver:

Cavagis ADM. Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação. [Internet] [Thesis]. Universidade Estadual de Campinas; 2005. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314033.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cavagis ADM. Modulação de MAPKs em celulas de leucemia humana induzidas a apoptose e diferenciação. [Thesis]. Universidade Estadual de Campinas; 2005. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314033

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

7. Figueiredo, Camila Alexandrina Viana de. Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes.

Degree: 2005, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311272

► Abstract: In the present study we investigated the protective effects of the oral administration of Green tea (GTE) (50, 100 and 250mg/Kg) in mice infected… (more)

Subjects/Keywords: Camellia sinensis; Extratos vegetais; Listeria monocytogenes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Figueiredo, C. A. V. d. (2005). Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/311272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Figueiredo, Camila Alexandrina Viana de. “Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes.” 2005. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Figueiredo, Camila Alexandrina Viana de. “Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes.” 2005. Web. 28 Feb 2021.

Vancouver:

Figueiredo CAVd. Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes. [Internet] [Thesis]. Universidade Estadual de Campinas; 2005. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Figueiredo CAVd. Avaliação dos efeitos do extrato do cha verde (Camellia sinensis (L.) Kuntze) sobre a resposta imunohematopoetica de camundongos infectados com Listeria monocytogenes. [Thesis]. Universidade Estadual de Campinas; 2005. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

8. Rodrigues, Ana Paula Ottati. Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes.

Degree: 2005, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311273

► Abstract: In this work, we investigated the effects of Chlorella vulgaris extract (CVE) in lead-exposed and Listeria monocytogenes infected mice. Experiments were carried out in… (more)

Subjects/Keywords: Chumbo; Celulas hematopoieticas primitivas; Alga; Terapia quelante; Imunologia; Listeriose

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APA (6^th Edition):

Rodrigues, A. P. O. (2005). Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/311273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodrigues, Ana Paula Ottati. “Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes.” 2005. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodrigues, Ana Paula Ottati. “Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes.” 2005. Web. 28 Feb 2021.

Vancouver:

Rodrigues APO. Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes. [Internet] [Thesis]. Universidade Estadual de Campinas; 2005. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodrigues APO. Avaliação dos efeitos do extrato de Chlorella vulgaris sobre a resposta imunologica do tipo celular em camundongos expostos ao chumbo e infectados com Listeria monocytogenes. [Thesis]. Universidade Estadual de Campinas; 2005. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/311273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

9. Freitas, Erika Maria Silva. Ação toxica da veratrina em mitocondrias e no reticulo sarcomplasmatico: Toxic action of veratrine on mitochondria and sarcoplasmatic reticulum.

Degree: 2006, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317746

► Abstract: The veratrine, a mixture of alkaloids obtained from plant Schoenocaulon officinale, is a lipid soluble toxin, which target voltage-gated Na+ channels for their primary… (more)

Subjects/Keywords: Células musculares; Mitocôndria; Canais de sódio; Veratrina; Reticulo sarcoplasmatico; Sodium channels; Veratrine; Muscle cells; Mitochondria; Sarcoplasmatic reticulum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Freitas, E. M. S. (2006). Ação toxica da veratrina em mitocondrias e no reticulo sarcomplasmatico: Toxic action of veratrine on mitochondria and sarcoplasmatic reticulum. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freitas, Erika Maria Silva. “Ação toxica da veratrina em mitocondrias e no reticulo sarcomplasmatico: Toxic action of veratrine on mitochondria and sarcoplasmatic reticulum.” 2006. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freitas, Erika Maria Silva. “Ação toxica da veratrina em mitocondrias e no reticulo sarcomplasmatico: Toxic action of veratrine on mitochondria and sarcoplasmatic reticulum.” 2006. Web. 28 Feb 2021.

Vancouver:

Freitas EMS. Ação toxica da veratrina em mitocondrias e no reticulo sarcomplasmatico: Toxic action of veratrine on mitochondria and sarcoplasmatic reticulum. [Internet] [Thesis]. Universidade Estadual de Campinas; 2006. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317746.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freitas EMS. Ação toxica da veratrina em mitocondrias e no reticulo sarcomplasmatico: Toxic action of veratrine on mitochondria and sarcoplasmatic reticulum. [Thesis]. Universidade Estadual de Campinas; 2006. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317746

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

10. Maróstica Junior, Mário Roberto, 1980-. Biotransformação de terpenos para a produção de compostos de aroma e funcionais: Biotransformation of terpenes in flavor and functional compounds.

Degree: 2006, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/256710

► Abstract: The biotransformation of terpenes and their functional properties were investigated in this study. The biotransformation of limonene was done by a Fusarium oxysporum strain… (more)

▼ Abstract: The biotransformation of terpenes and their functional properties were investigated in this study. The biotransformation of limonene was done by a Fusarium oxysporum strain grown in cassava waste water and transferred into a mineral medium for biotransformation. The limonene source was an orange essential oil from a orange juice industry. The main biotransformation product was a-terpineol, reaching around 450 mg/L. Similarly, biotransformation of citronellol was conducted by a Penicillium sp. strain grown also in cassava waste water and transferred into a mineral medium. The main product was cis-rose oxide, reaching concentrations higher than 70 mg/L. The screening of microrganisms for biotransformation of limonene (from orange essential oil) and a-, b-pinenes (from turpentine oil, residue from pulp industry) was done by solid phase microextraction (SPME). The technique was effective for the recovery of the volatiles from the headspace of sporulated surface biotransformation cultures. Liquid culture biotransformation experiments performed with the SPME screened strains resulted in the production of 50 mg/L of verbenol by Mucor sp. 2276 and 70 mg/L of verbenone form a-, b-pinenes and 90 mg/L of a-terpineol by Aspergillus sp. 2357 and 10 mg/L of perillyl alcohol by Penicillium sp. 2360. The biotransformation of a-farnesene by Aspergillus niger strains resulted in compounds never described in the literature before. Four main new compounds were obtained. Only one of them could be identified by GC-MS as 6-OH-farnesene. CG-O experiments revealed the impactant citrus aroma of 6-OH-farnesene. 'In vitro¿ and 'in vivo¿ experiments with the limonene biotransformation extract by Fusarium oxysporum and with the monoterpene standards present in the extract revealed the antioxidant potential of these compounds, which open a new perspective for the utilization of these compounds as functional aroma compounds Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Pastore, Glaucia Maria, 1953- (advisor), Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos (institution), Programa de Pós-Graduação em Ciência de Alimentos (nameofprogram), Zanin, Gisella Maria (committee member), Macedo, Gabriela Alves (committee member), Couri, Sonia (committee member), Hyslo, Stephen (committee member), Park, Yong Kun (committee member).

Subjects/Keywords: Compostos de aroma; Metabolismo; Fungos; Terpenos; Biotransformation; Aroma compounds; Fungi terpenes; Functional foods

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maróstica Junior, Mário Roberto, 1. (2006). Biotransformação de terpenos para a produção de compostos de aroma e funcionais: Biotransformation of terpenes in flavor and functional compounds. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/256710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maróstica Junior, Mário Roberto, 1980-. “Biotransformação de terpenos para a produção de compostos de aroma e funcionais: Biotransformation of terpenes in flavor and functional compounds.” 2006. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maróstica Junior, Mário Roberto, 1980-. “Biotransformação de terpenos para a produção de compostos de aroma e funcionais: Biotransformation of terpenes in flavor and functional compounds.” 2006. Web. 28 Feb 2021.

Vancouver:

Maróstica Junior, Mário Roberto 1. Biotransformação de terpenos para a produção de compostos de aroma e funcionais: Biotransformation of terpenes in flavor and functional compounds. [Internet] [Thesis]. Universidade Estadual de Campinas; 2006. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/256710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maróstica Junior, Mário Roberto 1. Biotransformação de terpenos para a produção de compostos de aroma e funcionais: Biotransformation of terpenes in flavor and functional compounds. [Thesis]. Universidade Estadual de Campinas; 2006. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/256710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

11. Malheiros, Sonia Valeria Pinheiro. Ação de xenobioticos em membranas biologicas e sinteticas.

Degree: 1999, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314589

► Abstract: The present work reports a study of the interaction of the antipsychotic trifluoperazine (TFP), the local anesthetic dibucaine (DBC) and the antihelminthic praziquantel (PZQ)… (more)

▼ Abstract: The present work reports a study of the interaction of the antipsychotic trifluoperazine (TFP), the local anesthetic dibucaine (DBC) and the antihelminthic praziquantel (PZQ) with biological and synthetic membranes. The drug-membrane interaction was studied by the determination of the partition coefficient of the drugs between various membrane systems and water, and by monitoring the hemolytic activity (determination of drugllipid ratios for protection and Iysis), determination of the thermal effect involved in hemolysis, morphological analysis and determination of the release of membrane lipids). In addition the study of TFP and DBC ionization contribution to membrane interaction was performed. TFP presented the higher partition coefficient in ali studied membranes and the higher hemolytic efficacy, followed by OSC and PZQ. The higher partition of TFP in relation to OSC is due to the specific interaction with membrane proteins and to the greater contribution of the neutral form at pH 7.4. The protection induced by the drugs in hyposmotic conditions is directly related to the compounds' hydrophobicity and it is triggered by the monomeric drug partition into membrane, in lower concentrations than those required for membrane saturation. The effective drugllipid ratio in the isosmotic hemolysis induced by TFP occurs before membrane saturation while for OSC and PZQ the Iytic drugllipid ratio reflect a real membrane saturation. The hydrofobicity seems to be a determinant factor in the induced Iysis although factors as TFP aggregative property and the low solubility of PZQ have became incisive to the induction or inhibition of hemolysis. The TFP and OSC induced Iysis are primarily enthalpyc process in which the events that more effectively contribute are the specific membrane protein interaction and micelle-mixed formation in case of TFP, and the monomer intercalation into membrane, for DBC. Ali the studied drugs induced stomatocytosis. The phospholipid and cholesterol release induced by the drugs are not directly related to hemolysis. The results presented suggest that distinct pathways are responsible for the Iytic effect induced for each drug and reveal the plurality of factors (hydrofobicity, solubility, ionization degree, aggregative properties, specific affinity for membrane components and steric effects) that must be considered in the interpretation of the hemolytic phenomenon or, in a more general view, the interaction of amphiphiles with biological membranes Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Meirelles, Nilce Correa, 1943- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Ciências Biológicas (nameofprogram), Ogo, Satie Hatsushika (committee member), Santana, Maria Helena Andrade (committee member), Volpe, Pedro Luis O. (committee member), Hyslo, Stephen (committee member).

Subjects/Keywords: Membranas (Biologia); Hemolise e hemolisinas; Sangue

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APA (6^th Edition):

Malheiros, S. V. P. (1999). Ação de xenobioticos em membranas biologicas e sinteticas. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314589

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Malheiros, Sonia Valeria Pinheiro. “Ação de xenobioticos em membranas biologicas e sinteticas.” 1999. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314589.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Malheiros, Sonia Valeria Pinheiro. “Ação de xenobioticos em membranas biologicas e sinteticas.” 1999. Web. 28 Feb 2021.

Vancouver:

Malheiros SVP. Ação de xenobioticos em membranas biologicas e sinteticas. [Internet] [Thesis]. Universidade Estadual de Campinas; 1999. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314589.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Malheiros SVP. Ação de xenobioticos em membranas biologicas e sinteticas. [Thesis]. Universidade Estadual de Campinas; 1999. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314589

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

12. Fan, Hui Wen. Ensaio clinico para avaliação da eficacia e segurança de um antiveneno especifico no tratamento da sindrome hemorragica causada por lagartas do genero Lonomia.

Degree: 2002, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309616

► Abstract: A clinical trial to evaluate the efficacy and safety of a specific antivenom against the hemorrhagic syndrome caused by Lonomia caterpillars. Disseminated bleeding after… (more)

▼ Abstract: A clinical trial to evaluate the efficacy and safety of a specific antivenom against the hemorrhagic syndrome caused by Lonomia caterpillars. Disseminated bleeding after the contact with caterpillars was first described in Brazil in 1912. In Venezuela, a hemorrhagic syndrome caused by larvae ofa moth ofthe Satuniidae family, namely, Lonomia achelous, has been reported since the 1960's, and similar cases were also reported in Amazon region. At the end of the eighties, a very high incidence of the bleeding syndrome has been described in the rural areas in the south of Brazil and the caterpillars were identified as Lonomia oblíqua. Patients experienced burning pain at the site of contact, followed by general discornfort, headache and nausea, and in some hours afier contact, hemorrhagic manifestations: bleeding from skin and mucous membranes, epistaxis, hematuria, hematemesis, pulmonary bleeding. Acute renal failure and intracerebral bleeding were associated with fatal outcomes. In a short time, due to severity and progression of cases, Lonomia caterpillar envenoming was therefore considered a new public health problem. As antivenoms are effective therapy for treatment of snake bites, a process of manufacturing a specific Lonomia antivenom (SALon) was developed by immunizing horses with Lonomia caterpillar bristle extracts. The safety and efficacy of this new antivenom has been tested in a three phase randomized open c1inicaltrial at the Hospital São Vicente de Paulo, Passo Fundo, RS. In phase I, 15 patients received different amounts of SALon and three presented anaphylactic/anaphylactoid reactions (urticaria in two and brochospasm in one). Frequency and severity of early reactions were not exceptionally different in either ofthe next phases, confirming its safety. Patients in phase II, graded according to severity of envenoming, were allocated in two groups: SALon or epsilon-amino-caproic acid (EACA), an antillbrinolytic drug extensively used in Lonomia cases in Venezuela. The study was interrupted when a patient in group EACA died 64 days afier hospitalization in consequence of acute renal failure and infectious complications. At that time, 9 patients had received SALon and 8 EACA. The comparison showed an evident superiority of SALon in reversing the consumptive coagulopathy, measured by Coagulation Test, Prothrombin Time and dosage of plasma fibrinogen. The decision was not to deprive any patient of antivenom therapy and, in phase III, the efficacy of the association between SALon with EACA was assayed. Patients receiving SALon (n = 58) or SALon + EACA (n = 56) showed to be similar in terms the time needed to normalize coagulation parameters, which occurred within 24 hours afier antivenom therapy in most of patients. The sequential reanalysis of data, applying the triangular test, gave a substantial reduction in the number of patients required, for the same conclusions reached with the completed study. The trial was fmished concluding that antivenom is efficacy in the treatment of Lonomia caterpillar… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Corrêa Filho, Heleno Rodrigues, 1950- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Saúde Coletiva (nameofprogram), Sano-Martins, Ida Sigueko (committee member), Barros, Elvino Jose Guardão (committee member), Hyslo, Stephen (committee member), Moreira Filho, Djalma de Carvalho (committee member).

Subjects/Keywords: Antivenenos; Doenças hemorragicas; Lagarta; Análise sequêncial

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fan, H. W. (2002). Ensaio clinico para avaliação da eficacia e segurança de um antiveneno especifico no tratamento da sindrome hemorragica causada por lagartas do genero Lonomia. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/309616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fan, Hui Wen. “Ensaio clinico para avaliação da eficacia e segurança de um antiveneno especifico no tratamento da sindrome hemorragica causada por lagartas do genero Lonomia.” 2002. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/309616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fan, Hui Wen. “Ensaio clinico para avaliação da eficacia e segurança de um antiveneno especifico no tratamento da sindrome hemorragica causada por lagartas do genero Lonomia.” 2002. Web. 28 Feb 2021.

Vancouver:

Fan HW. Ensaio clinico para avaliação da eficacia e segurança de um antiveneno especifico no tratamento da sindrome hemorragica causada por lagartas do genero Lonomia. [Internet] [Thesis]. Universidade Estadual de Campinas; 2002. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fan HW. Ensaio clinico para avaliação da eficacia e segurança de um antiveneno especifico no tratamento da sindrome hemorragica causada por lagartas do genero Lonomia. [Thesis]. Universidade Estadual de Campinas; 2002. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/309616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

13. Pando, Silvana Cristina. Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor.

Degree: 2003, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656

► Abstract: The aims of this work were to purify and to characterize biochemically a lectin and to determine the primary structure of a Kunitz-type serineproteinase… (more)

▼ Abstract: The aims of this work were to purify and to characterize biochemically a lectin and to determine the primary structure of a Kunitz-type serineproteinase inhibitor from seeds of Delonix regia specie (Leguminosae-Caesalpinioideae) and also to verify the in vitro effect of the inhibitor towards to digestive enzymes activities of the lepidoptera insects A. kuehniella and C. cephalonica. For lectin and serine proteinase inhibitor purification, the seeds were tritured and the flour was submitted to saline extraction 10%. After centrifugation , the supernatant (total extract) was dialised, Iyophylized and applied in molecular exclusion, ion exchange and reverse phase C18 HPLC columns. The eluted fractions were monitored by absorbance at 280 nm. The lectin and the inhibitor purified were designated DRL (Delonix regia lectin) and DrTI (Delonix regia trypsin inhibitor), respectively. After identification and purification, the lectin was analyzed in SDS-PAGE 12.5%, showing that this protein has an apparent molecular mass of 12 kDa under reduced (DTT 0.1 M) and non reduced conditions. Analysis of native gel revealed that DRL is a protein constituted by only one polypeptide chain. The inhibition tests of hemagglutinating activity were realized with rat erytrocytes, using the following sugars: D-glucose, D-glucosamine, D-fructose, Dmannose, D-sucrose, D-galactose, D-galactosamine, D-raffinose, D-Iactose and Dmaltose and we verified that hemagglutinating activity of DRL was more specifically inhibited by glucose at 0.048 mM concentration, indicating this lectin belongs to the glucose-mannose group. The lectin aminoacid composition showed high content of aspartic acid, glutamic acid and glycine, but cysteine residue was not detected. The determination of the N-terminal region of the lectin revealed high homology with other leguminous lectins such as, con A, lentil and pea. The pH range for the lectin activity was 8-9 and DRL lost activity totally in range of acid pH (2-4). Furthermore, the lectin maintained activity after thermal treatment to 70°C, during 30 minutes, with lost of 50% in activity at 100°C. DRL does not require calcium for the activity but is manganese dependent, suggesting that DRL is a metalloprotein. The treatment of DRL with urea resulted in total lost of hemagglutinating activity, while in the presence of DTT, the lectin maintained normal activity probably due absence disulfide bridges in the molecule. The primary structure determination of DrTI, proved that this inhibitor belongs to the Kunitz family of proteinase inhibitors. The region of reactive site presented a glutamic acid residue instead of an arginine or Iysine, but the molecule presented two "loops", composed by four cysteine residues, with high similarity with other Kunitz type inhibitors. The in vitro inhibition studies with insect proteases from A. kuehniella and C. cephalonica showed at concentration of only 5mg, DrTI inhibited about of 92% of the proteases these insects, indicating high affinity of the inhibitor by these enzymes. In vitro… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Marangoni, Sérgio, 1951- (other), Macedo, Maria Lígia Rodrigues (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Hyslo, Stephen (committee member), Oliva, Maria Luiza Vilela (committee member), Carlini, Celia Regina Ribeiro da Silva (committee member), Gadelha, Fernanda Ramos (committee member).

Subjects/Keywords: Lectinas; Sementes; Lepdoptero

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pando, S. C. (2003). Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pando, Silvana Cristina. “Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor.” 2003. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pando, Silvana Cristina. “Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor.” 2003. Web. 28 Feb 2021.

Vancouver:

Pando SC. Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor. [Internet] [Thesis]. Universidade Estadual de Campinas; 2003. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pando SC. Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor. [Thesis]. Universidade Estadual de Campinas; 2003. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

14. Oliveira, Caroline Ribeiro de Borja. Ação neurotoxica do veneno de Bothrops neuwiedi pauloensis (jararaca-pintada) em preparações neuromusculares isoladas : purificação, caracterização parcial e sequencia da porção amino-terminal de duas PLA2 neurotoxicas pre-sinapticas : neuwieditoxinas-I e II.

Degree: 2002, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310310

► Abstract: Snakes of the genus Bothrops are the most important cause of snakebites in Brazil. The main complications in lethal cases are acute renal failure,… (more)

▼ Abstract: Snakes of the genus Bothrops are the most important cause of snakebites in Brazil. The main complications in lethal cases are acute renal failure, shock, acute respiratory failure and sepsis. The neuromuscular effects of Bothrops neuwiedi pauloensis (jararaca-pintada) venom were studied in chick biventer cervicis and mouse phrenic nerve-diaphragm preparations. In chick preparations, venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary augmentation of the twitches evoked indirectly followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to KCI (13.4 mM) were partially or completely inhibited whereas those to acetylcholine (110 µg/Mw) ere unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCI and direct stimulation. At 20-24°C, the venom (50 µg/ml)produced only partial neuromuscular blockade (30.7± 8.0% blockade, mean:t SEM; p < 0.05; n = 3) atter 120 min and the initial inhibition and the secondary augmentation of the twitch responses caused by the venom were prolonged and pronounced and the response to KCI was unaltered. In mouse preparation, At 20 µg/ml,the venom irreversibly inhibited (60 ±10% inhibition, mean ± SEM; p< 0.05; n = 6) indirectly evoked twitches. At 50 µg/ml, the venom blocked the indirectly (n = 5) and directly (n = 6) evoked twitches in mouse hemidiaphragms. In the absence of Ca2+, at 50 µg/ml,only partiaI blockade (40.3v ± 7.8% inhibition; p < 0.05; n = 3) was observed atter a 120 min incubation.These results indicated that B. n. pauloensís venom was neurotoxic, acting primarily at presynaptic sites, and that enzymatic activity could be involved in that pharmacological action. The venom was fractionated by HPLC molecular exclusion (Protein Pack 300SW column). The neuromuscular activity of the venom was recovered in the first, second and third peaks (peak 1, peak 2 and peak 3, respectively) of the elution profile.Note: The complete abstract is available with the full electronic digital thesis or dissertations Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Simioni, Lea Rodrigues, 1942- (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Farmacologia (nameofprogram), Fontana, Marcos Dias (committee member), Oliveira, Antonio Carlos de (committee member), Hyslo, Stephen (committee member), Furtado, Maria de Fatima Domingues (committee member).

Subjects/Keywords: Junção neuromuscular; Fosfolipases; Neurotoxicologia; Cobra venenosa; Bioquímica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, C. R. d. B. (2002). Ação neurotoxica do veneno de Bothrops neuwiedi pauloensis (jararaca-pintada) em preparações neuromusculares isoladas : purificação, caracterização parcial e sequencia da porção amino-terminal de duas PLA2 neurotoxicas pre-sinapticas : neuwieditoxinas-I e II. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/310310

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Caroline Ribeiro de Borja. “Ação neurotoxica do veneno de Bothrops neuwiedi pauloensis (jararaca-pintada) em preparações neuromusculares isoladas : purificação, caracterização parcial e sequencia da porção amino-terminal de duas PLA2 neurotoxicas pre-sinapticas : neuwieditoxinas-I e II.” 2002. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310310.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Caroline Ribeiro de Borja. “Ação neurotoxica do veneno de Bothrops neuwiedi pauloensis (jararaca-pintada) em preparações neuromusculares isoladas : purificação, caracterização parcial e sequencia da porção amino-terminal de duas PLA2 neurotoxicas pre-sinapticas : neuwieditoxinas-I e II.” 2002. Web. 28 Feb 2021.

Vancouver:

Oliveira CRdB. Ação neurotoxica do veneno de Bothrops neuwiedi pauloensis (jararaca-pintada) em preparações neuromusculares isoladas : purificação, caracterização parcial e sequencia da porção amino-terminal de duas PLA2 neurotoxicas pre-sinapticas : neuwieditoxinas-I e II. [Internet] [Thesis]. Universidade Estadual de Campinas; 2002. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310310.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira CRdB. Ação neurotoxica do veneno de Bothrops neuwiedi pauloensis (jararaca-pintada) em preparações neuromusculares isoladas : purificação, caracterização parcial e sequencia da porção amino-terminal de duas PLA2 neurotoxicas pre-sinapticas : neuwieditoxinas-I e II. [Thesis]. Universidade Estadual de Campinas; 2002. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310310

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

15. Paula, Fernanda Borges de Araujo. Atividade do extrato de frutos de Pterodon emarginatus contra o estresse oxidativo e nitrosativo induzido por exercicio agudo em ratos.

Degree: 2004, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314340

► Abstract: High-intensity exercise can cause muscle damage. Evidences point to a strong association between physical exercise-induced muscle damage and reactive oxygen (ROS) and nitrogen (RNS)… (more)

▼ Abstract: High-intensity exercise can cause muscle damage. Evidences point to a strong association between physical exercise-induced muscle damage and reactive oxygen (ROS) and nitrogen (RNS) species. Animal studies have shown that phytochemical antioxidants, such as terpens and phenols are capable of removing free radicals. Previous chemical analysis of the hexane crude extract (HCE) of Peterodon emarginatus showed the presence of the acid 6?, 7?-dihydroxyvouacapane-17?-oic (diterpenoid), ?-cariophilene, ?-pinene, myrcene, eugenol and geraniol. The aim of the present work was to evaluate the effect of HCE in oxidative and nitrosative stress induced by intense exercise in brain, liver and tibialis anterior muscle of rats. Adult male Wistar rats were submitted to standardized contractions by functional electrical stimulation (FES) of the anterior tibialis muscles (50 Hz, 15 min). Animals were subjected to one doses (30 min before or 30 min after exercise) or three doses (24h, 12h and 30 min before exercise) of 498 mg/kg HCE oral administration. Serum creatine kinase (CK) activities were measured by kinetic method. Lipoperoxidation was estimated by measuring of thiobarbituric acid-reactive substances (TBARS). NO production was evaluated by measuring nitrite formation, using Griess reagent and tyrosine nitration was assessed by western blot analysis. In vitro studies demonstrated that HCE inhibited guaiacol oxidation (IC50=19,8?g/mL) as well as brain and muscle lipid peroxidation with IC50 of 16,2 ?g/mL and 26,7?g/mL, respectively. The extract affected nitric oxide synthase (NOS) activity in brain (IC50=49?g/mL) and muscle (IC50=42,3?g/mL) homogenates of adult Wistar rats. Nitrite production in brain and muscle tissue homogenates decreased in the presence of NOS inhibitors (NG-nitro-L-arginine methyl ester and aminoguanidine) and was even lower in the presence of HCE. The in vivo studies demonstrated that serum CK activities in the controls (111U/L) increased after 1h FES (443U/L). Intense exercise also increased the lipid peroxidation in the brain (108,9%), liver (55,5%) and tibialis anterior muscle (967%), and also nitrite levels in 90,5%, 30,7%, 60%, respectively. The tyrosine nitration induced by exercise was identified in these tissues. Previous treatment with HCE decreased the lipoperoxidation, nitrite production and also the tyrosine nitration leading to the levels of controls. This effect could be explained by the chemical composition of this extract, that has phenols and terpens, which exhibit free radical scavenging activity and the present results also indicate that EHB has also NOS inhibitory properties Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Salgado, Ione, 1953- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Funcional e Molecular (nameofprogram), Hyslo, Stephen (committee member), Moreno, Ronilson Aguinaldo (committee member), Ferreira, Carmen Veríssima (committee member), Bernardes, Celene Fernandes (committee member).

Subjects/Keywords: Estresse oxidativo; Músculos - Ferimentos e lesões

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paula, F. B. d. A. (2004). Atividade do extrato de frutos de Pterodon emarginatus contra o estresse oxidativo e nitrosativo induzido por exercicio agudo em ratos. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paula, Fernanda Borges de Araujo. “Atividade do extrato de frutos de Pterodon emarginatus contra o estresse oxidativo e nitrosativo induzido por exercicio agudo em ratos.” 2004. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paula, Fernanda Borges de Araujo. “Atividade do extrato de frutos de Pterodon emarginatus contra o estresse oxidativo e nitrosativo induzido por exercicio agudo em ratos.” 2004. Web. 28 Feb 2021.

Vancouver:

Paula FBdA. Atividade do extrato de frutos de Pterodon emarginatus contra o estresse oxidativo e nitrosativo induzido por exercicio agudo em ratos. [Internet] [Thesis]. Universidade Estadual de Campinas; 2004. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paula FBdA. Atividade do extrato de frutos de Pterodon emarginatus contra o estresse oxidativo e nitrosativo induzido por exercicio agudo em ratos. [Thesis]. Universidade Estadual de Campinas; 2004. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

16. Albuquerque, Elaine Christine de Magalhães Cabral. Preparação, caracterização de lipossomas e microesferas para aplicação na terapia por dessensibilização de reação alergica: Preparation and characterization of liposomes and microspheres useful for desensitization therapy of allergic reaction.

Degree: 2005, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/267213

► Abstract: Respiratory allergic disorders affect a great number of individuals throughout the world. Nowadays, conventional therapies for atopic individuals comprise specific immunotherapy and pharmacotherapy. In… (more)

▼ Abstract: Respiratory allergic disorders affect a great number of individuals throughout the world. Nowadays, conventional therapies for atopic individuals comprise specific immunotherapy and pharmacotherapy. In general, the immunotherapy using free allergens produces adverse systemic reactions of varying intensity and frequency. In order to circumvent these problems, several approaches have been explored. Among them, liposomes and microspheres have been studied as promising vehicles as carriers of allergens for desensitization immunotherapy in allergy. Liposomes are small phospholipid vesicles composed of nontoxic, biodegradable, and naturally occurring immune response and producing a pronounced depot effect after subcutaneous injection. Microspheres have also been widely investigated in drug delivery studies due to their capability of presenting antigens to the immune system over a long time. A large number of natural and synthetic polymers are potentially suitable for production of the wall-forming polymer. Synthetic biodegradable poly (lactide-co-glycolide) copolymers are among the primary candidates due to their excellent tissue biocompatibility, biodegradability and regulatory approval. The aim of this work is to prepare and characterize liposomes and micrspheres entrapping allergenic extract from Dechlera (Helminthosporium) monoceras and ovoalbumin. The in vivo effects of the sequential administration of both asjuvants containing ovoalbumin were also evaluated by analysis of immunoglobulin G (IgG) and immunoglobulin E (IgE) levels...Note: The complete abstract is available with the full electronic digital thesis or dissertations Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Santana, Maria Helena Andrade, 1951- (advisor), Zollner, Ricardo de Lima, 1954- (coadvisor), Universidade Estadual de Campinas. Faculdade de Engenharia Química (institution), Programa de Pós-Graduação em Engenharia Química (nameofprogram), Costa, Maria Helena Bueno da (committee member), Oliveira, Anselmo Gomes de (committee member), Hyslo, Stephen (committee member), Pessine, Francisco Benedito Teixeira (committee member).

Subjects/Keywords: Lipossomos; Partículas; Polímeros na medicina; Alergia respiratoria; Lipossomes; Particles; Biomedical polymers; Respiratory allergy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albuquerque, E. C. d. M. C. (2005). Preparação, caracterização de lipossomas e microesferas para aplicação na terapia por dessensibilização de reação alergica: Preparation and characterization of liposomes and microspheres useful for desensitization therapy of allergic reaction. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/267213

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albuquerque, Elaine Christine de Magalhães Cabral. “Preparação, caracterização de lipossomas e microesferas para aplicação na terapia por dessensibilização de reação alergica: Preparation and characterization of liposomes and microspheres useful for desensitization therapy of allergic reaction.” 2005. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267213.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albuquerque, Elaine Christine de Magalhães Cabral. “Preparação, caracterização de lipossomas e microesferas para aplicação na terapia por dessensibilização de reação alergica: Preparation and characterization of liposomes and microspheres useful for desensitization therapy of allergic reaction.” 2005. Web. 28 Feb 2021.

Vancouver:

Albuquerque ECdMC. Preparação, caracterização de lipossomas e microesferas para aplicação na terapia por dessensibilização de reação alergica: Preparation and characterization of liposomes and microspheres useful for desensitization therapy of allergic reaction. [Internet] [Thesis]. Universidade Estadual de Campinas; 2005. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/267213.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albuquerque ECdMC. Preparação, caracterização de lipossomas e microesferas para aplicação na terapia por dessensibilização de reação alergica: Preparation and characterization of liposomes and microspheres useful for desensitization therapy of allergic reaction. [Thesis]. Universidade Estadual de Campinas; 2005. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/267213

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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