Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for +publisher:"Universidade Estadual de Campinas" +contributor:("Gontijo, Antonio Rocha"). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Universidade Estadual de Campinas

1. Belline, Paula. Ação de angiotensina II sobre macrofagos peritoneais de camundongos normais e diabeticos.

Degree: 2001, Universidade Estadual de Campinas

Abstract: The Renin Angiotensin System (RAS) is an important regulatory mechanism of cardiovascular homeostasis in normal and hypertensive subjects. Recently, several reports have been showed the existence of a local RAS in different cells and tissues, inclusive immunological cells. The aim of this study was evaluated in culture the effect of Angiotensin II (AII) on peritoneal mice macrophages (BALB/c and Non Obese Diabetic - NOD). The dose response curve of AII on phagocytic activity was determined during 1 and 3 hours of treatment and the stimulant dose of the phagocytosis was AII 10-12 M. To verify if this stimulus was obtained through AT1 receptor, the dose response curve of DuP 753 was determined. The protocol with DuP 753 was realized in the presence and absence of AII. The results showed that the inhibition of the phagocytic activity occurred in different concentrations of DuP, but the higher inhibition, in normal and diabetic animals, it was with 10-6 M. In order to evaluate the cytotoxic effect of DuP 753 and to show that the inhibition effect was not caused by an alteration of the cellular viability, were realized 3 cytotoxic assays: MTT reduction, analysis of nucleic acid content (NAC) and lactate dehydrogenase release (LDH). All assays indicated that cell viability was maintained afier treatment with DuP 753 in concentrations used. In addition of the phagocytic activity, the effect of AII and DuP 753 in production of superoxide anions was also investigate through the ferricytochrome c reduction. The results demonstrated that the phagocytic stimulant dose of AII (10-12 M) and the inhibition phagocytic dose of DuP (10-6 M) did not influence the superoxide production of macrophages. These results showed that the phagocytic function and superoxide production are probably activated by different concentrations of AII. Finally, an immunofluorescence study was performed in order to identify the type of AII receptors are present in BALB/c and NOD macrophages. The treatment of the cells with AII and DuP changed the immunoreactive of AT1 and AT2 receptors. This shows the influence of RAS on the function and metabolism of lmmunological Cell System Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Figueiredo, Jose Francisco, 1942- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Funcional e Molecular (nameofprogram), Ferreira, Carmen Veríssima (committee member), Gadelha, Fernanda Ramos (committee member), Gontijo, Antonio Rocha (committee member), Coimbra, Terezila Machado (committee member).

Subjects/Keywords: Macrofagos; Angiotensina; Diabetes Mellitus; Fagocitose

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Belline, P. (2001). Ação de angiotensina II sobre macrofagos peritoneais de camundongos normais e diabeticos. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314389

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Belline, Paula. “Ação de angiotensina II sobre macrofagos peritoneais de camundongos normais e diabeticos.” 2001. Thesis, Universidade Estadual de Campinas. Accessed October 31, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314389.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Belline, Paula. “Ação de angiotensina II sobre macrofagos peritoneais de camundongos normais e diabeticos.” 2001. Web. 31 Oct 2020.

Vancouver:

Belline P. Ação de angiotensina II sobre macrofagos peritoneais de camundongos normais e diabeticos. [Internet] [Thesis]. Universidade Estadual de Campinas; 2001. [cited 2020 Oct 31]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314389.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belline P. Ação de angiotensina II sobre macrofagos peritoneais de camundongos normais e diabeticos. [Thesis]. Universidade Estadual de Campinas; 2001. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314389

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.