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You searched for +publisher:"Universidade Estadual de Campinas" +contributor:("Carlini, Celia Regina Ribeiro da Silva"). One record found.

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Universidade Estadual de Campinas

1. Pando, Silvana Cristina. Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor.

Degree: 2003, Universidade Estadual de Campinas

Abstract: The aims of this work were to purify and to characterize biochemically a lectin and to determine the primary structure of a Kunitz-type serineproteinase inhibitor from seeds of Delonix regia specie (Leguminosae-Caesalpinioideae) and also to verify the in vitro effect of the inhibitor towards to digestive enzymes activities of the lepidoptera insects A. kuehniella and C. cephalonica. For lectin and serine proteinase inhibitor purification, the seeds were tritured and the flour was submitted to saline extraction 10%. After centrifugation , the supernatant (total extract) was dialised, Iyophylized and applied in molecular exclusion, ion exchange and reverse phase C18 HPLC columns. The eluted fractions were monitored by absorbance at 280 nm. The lectin and the inhibitor purified were designated DRL (Delonix regia lectin) and DrTI (Delonix regia trypsin inhibitor), respectively. After identification and purification, the lectin was analyzed in SDS-PAGE 12.5%, showing that this protein has an apparent molecular mass of 12 kDa under reduced (DTT 0.1 M) and non reduced conditions. Analysis of native gel revealed that DRL is a protein constituted by only one polypeptide chain. The inhibition tests of hemagglutinating activity were realized with rat erytrocytes, using the following sugars: D-glucose, D-glucosamine, D-fructose, Dmannose, D-sucrose, D-galactose, D-galactosamine, D-raffinose, D-Iactose and Dmaltose and we verified that hemagglutinating activity of DRL was more specifically inhibited by glucose at 0.048 mM concentration, indicating this lectin belongs to the glucose-mannose group. The lectin aminoacid composition showed high content of aspartic acid, glutamic acid and glycine, but cysteine residue was not detected. The determination of the N-terminal region of the lectin revealed high homology with other leguminous lectins such as, con A, lentil and pea. The pH range for the lectin activity was 8-9 and DRL lost activity totally in range of acid pH (2-4). Furthermore, the lectin maintained activity after thermal treatment to 70°C, during 30 minutes, with lost of 50% in activity at 100°C. DRL does not require calcium for the activity but is manganese dependent, suggesting that DRL is a metalloprotein. The treatment of DRL with urea resulted in total lost of hemagglutinating activity, while in the presence of DTT, the lectin maintained normal activity probably due absence disulfide bridges in the molecule. The primary structure determination of DrTI, proved that this inhibitor belongs to the Kunitz family of proteinase inhibitors. The region of reactive site presented a glutamic acid residue instead of an arginine or Iysine, but the molecule presented two "loops", composed by four cysteine residues, with high similarity with other Kunitz type inhibitors. The in vitro inhibition studies with insect proteases from A. kuehniella and C. cephalonica showed at concentration of only 5mg, DrTI inhibited about of 92% of the proteases these insects, indicating high affinity of the inhibitor by these enzymes. In vitro… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Marangoni, Sérgio, 1951- (other), Macedo, Maria Lígia Rodrigues (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Hyslo, Stephen (committee member), Oliva, Maria Luiza Vilela (committee member), Carlini, Celia Regina Ribeiro da Silva (committee member), Gadelha, Fernanda Ramos (committee member).

Subjects/Keywords: Lectinas; Sementes; Lepdoptero

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APA (6th Edition):

Pando, S. C. (2003). Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Pando, Silvana Cristina. “Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor.” 2003. Thesis, Universidade Estadual de Campinas. Accessed February 28, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Pando, Silvana Cristina. “Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor.” 2003. Web. 28 Feb 2021.

Vancouver:

Pando SC. Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor. [Internet] [Thesis]. Universidade Estadual de Campinas; 2003. [cited 2021 Feb 28]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pando SC. Caracterização bioquimica de uma lectina e determinação da estrutura primaria de um inibidor de serinoproteinases tipo Kunitz de sementes de Delonix regia(Flamboyant) : estudo in vitro da ação inseticida do inibidor. [Thesis]. Universidade Estadual de Campinas; 2003. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314656

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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