+publisher:"Universidade Estadual de Campinas" +contributor:("Blotta, Maria Heloisa Souza Lima")

Universidade Estadual de Campinas

1. Fornazim, Marcia Cristina. Caracterização fenotípica e funcional de células dendríticas humanas induzidas por células leveduriformes de Paracoccidioides brasiliensis: Phenotypic and functional characterization of human dendritic cells induced by Paracoccidioides brasiliensis yeast cells.

Degree: 2011, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310982

► Abstract: A Th1 response with an efficient activation of macrophages is the main mechanism of defense in Paracoccidioides brasiliensis infection. Components of the innate immune… (more)

▼ Abstract: A Th1 response with an efficient activation of macrophages is the main mechanism of defense in Paracoccidioides brasiliensis infection. Components of the innate immune system that induce the polarization of the adaptive response such as dendritic cells (DCs) and their products have been little explored, particularly in human disease. The aim of this study was to phenotypically and functionally characterize DCs obtained from peripheral blood monocytes (PBM) of healthy individuals (C), patients with the adult form (AF) and juvenile form (JF) of paracoccidioidomycosis (PCM), induced in vitro by P. brasiliensis yeasts cells of Pb18 (high virulence) and Pb265 (low virulence) strains. In a second step we sought to determine whether these DCs were capable of activating autologous CD4/CD8 T cells. PBM were cultured in the presence of IL-4 and GM-CSF for 7 days and then stimulated by yeasts cells (Pb18 or Pb265) or CD40L (positive control) or medium only (WE), for 18 hours. PBM of the 3 groups (C, AF, JF) acquired the capacity to differentiate into DCs in the presence of P. brasiliensis yeasts cells. In patients with FA and FJ yeast cells induced maturation of DCs with increased expression of maturation markers (CD83 and CCR7), co-stimulatory molecules(CD80/B7-1 and CD86/B7-2), antigen presentation molecules (HLA-I and HLA-II), receptors involved in antigen recognition (TLR2, TLR4 and dectin-1) and decreased expression of CD1a and CD209. DCs from group C showed the same phenotypic characteristics, more evident in DCs that were stimulated with Pb265 when compared to Pb18. DCs stimulated with CD40L showed maximum expression of surface markers. A decrease in phagocytic capacity was detected in DCs of the control group after stimulation with yeast cells, demonstrating the maturation process, which was more effective in the presence of Pb265. The gene expression analysis showed that both strains of yeasts cells induced expression of TNF- , IL-1 , IL-12p35, IL-23p19 and TGF- mRNA. Stimulation of DCs with Pb265 induced increased expression of IL-6 in all groups. Pb265 also induced a higher expression of IL-10 mRNA only in patients (AF and JF). Our study also showed that phenotypically competent DCs were able to stimulate the proliferation of autologous CD4+/CD8+ cells, mainly in patients. In these groups (FA and FJ) it was also observed a reduction in the frequency of CD4+/CD8+/CD45RA+ and na increased frequency of CD4+/CD8+/CD45RO+ and CD4+/CD8+/CD69+. Unexpectedly, the results also showed that DCs stimulated with the yeast cells induced proliferation of T lymphocytes CD3+CD4+CD25+Foxp3+, compared to DCs that received no stimulation. DCs from patients stimulated by both strains of yeast cells induced a higherr production of IL-2, IL-17, IL-10 e IFN- by LT/CD3+CD8(neg) compared with DCs from group C. Moreover, DCs from the 3 groups also induced a stronger IFN-? production by CD3+/CD8+T cells. Altogether the results showed that stimulation with P. brasiliensis yeast cells induces a heterogeneous population of DCs, capable… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Blotta, Maria Heloisa de Souza Lima, 1953- (advisor), Blotta, Maria Heloisa Souza Lima (advisor), Mamoni, Ronei Luciano (coadvisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Ciências Médicas (nameofprogram), Burger, Eva (committee member), Almeida, Sandro Rogerio de (committee member), Giorgio, Selma (committee member), Pereira, Ricardo Mendes (committee member).

Subjects/Keywords: Paracoccidioidomicose; Paracoccidioides; Células dendríticas; Linfócitos T; Citocinas; Paracoccidioidomycosis; Paracoccidioides brasiliensis; Dendritic cells; T lymphocytes; Cytokines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fornazim, M. C. (2011). Caracterização fenotípica e funcional de células dendríticas humanas induzidas por células leveduriformes de Paracoccidioides brasiliensis: Phenotypic and functional characterization of human dendritic cells induced by Paracoccidioides brasiliensis yeast cells. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/310982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fornazim, Marcia Cristina. “Caracterização fenotípica e funcional de células dendríticas humanas induzidas por células leveduriformes de Paracoccidioides brasiliensis: Phenotypic and functional characterization of human dendritic cells induced by Paracoccidioides brasiliensis yeast cells.” 2011. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fornazim, Marcia Cristina. “Caracterização fenotípica e funcional de células dendríticas humanas induzidas por células leveduriformes de Paracoccidioides brasiliensis: Phenotypic and functional characterization of human dendritic cells induced by Paracoccidioides brasiliensis yeast cells.” 2011. Web. 25 Feb 2021.

Vancouver:

Fornazim MC. Caracterização fenotípica e funcional de células dendríticas humanas induzidas por células leveduriformes de Paracoccidioides brasiliensis: Phenotypic and functional characterization of human dendritic cells induced by Paracoccidioides brasiliensis yeast cells. [Internet] [Thesis]. Universidade Estadual de Campinas; 2011. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fornazim MC. Caracterização fenotípica e funcional de células dendríticas humanas induzidas por células leveduriformes de Paracoccidioides brasiliensis: Phenotypic and functional characterization of human dendritic cells induced by Paracoccidioides brasiliensis yeast cells. [Thesis]. Universidade Estadual de Campinas; 2011. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Bachur, Luis Felipe, 1981-. Avaliação dos níveis séricos de 1-3-B-D-Glucana e da resposta inflamatória em pacientes sépticos comparativamente a pacientes com candidíase invasiva: Evaluation of serum levels of 1-3-B-D-Glucan and the inflammatory response in septic patients compared to patients with invasive candidiasis.

Degree: 2019, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335734

► Abstract: Introduction: Early diagnosis remains as a major challenge for Invasive Candidiasis (IC) management and 1-3-"beta"-D-Glucan (BDG) is a useful biomarker. Nevertheless, there are still… (more)

Subjects/Keywords: Candidíase invasiva; Candidemia; Sepse; Glucana Endo-1,3-beta-D-Glucosidase; Citocinas; Interleucina-10; Invesive candidiasis; Candidemia; Sepsis; Glucan endo-1,3-beta-D-glucosidase; Cytokines; Interleukin-10

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bachur, Luis Felipe, 1. (2019). Avaliação dos níveis séricos de 1-3-B-D-Glucana e da resposta inflamatória em pacientes sépticos comparativamente a pacientes com candidíase invasiva: Evaluation of serum levels of 1-3-B-D-Glucan and the inflammatory response in septic patients compared to patients with invasive candidiasis. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/335734

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bachur, Luis Felipe, 1981-. “Avaliação dos níveis séricos de 1-3-B-D-Glucana e da resposta inflamatória em pacientes sépticos comparativamente a pacientes com candidíase invasiva: Evaluation of serum levels of 1-3-B-D-Glucan and the inflammatory response in septic patients compared to patients with invasive candidiasis.” 2019. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/335734.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bachur, Luis Felipe, 1981-. “Avaliação dos níveis séricos de 1-3-B-D-Glucana e da resposta inflamatória em pacientes sépticos comparativamente a pacientes com candidíase invasiva: Evaluation of serum levels of 1-3-B-D-Glucan and the inflammatory response in septic patients compared to patients with invasive candidiasis.” 2019. Web. 25 Feb 2021.

Vancouver:

Bachur, Luis Felipe 1. Avaliação dos níveis séricos de 1-3-B-D-Glucana e da resposta inflamatória em pacientes sépticos comparativamente a pacientes com candidíase invasiva: Evaluation of serum levels of 1-3-B-D-Glucan and the inflammatory response in septic patients compared to patients with invasive candidiasis. [Internet] [Thesis]. Universidade Estadual de Campinas; 2019. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335734.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bachur, Luis Felipe 1. Avaliação dos níveis séricos de 1-3-B-D-Glucana e da resposta inflamatória em pacientes sépticos comparativamente a pacientes com candidíase invasiva: Evaluation of serum levels of 1-3-B-D-Glucan and the inflammatory response in septic patients compared to patients with invasive candidiasis. [Thesis]. Universidade Estadual de Campinas; 2019. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/335734

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

3. Blanco, Marcos Luengo. O papel do fator nuclear Kappa B (NF-k B) na expressão do gene CYBB do sistema NADPH oxidase humano: The role of nuclear factor k-B (NF-kB) CYBB gene expression activity of the human NADPH oxidase system.

Degree: 2008, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308285

► Abstract: This work investigated the functional role of nuclear factor-? B (NF-?B) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine… (more)

Subjects/Keywords: NADPH oxidase - Análise; Superóxido; Expressão gênica; Doença Granuomatosa cronica; NADPH oxidase - Analysis; Superoxide; Gene expression; Chronic granulomatosis disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Blanco, M. L. (2008). O papel do fator nuclear Kappa B (NF-k B) na expressão do gene CYBB do sistema NADPH oxidase humano: The role of nuclear factor k-B (NF-kB) CYBB gene expression activity of the human NADPH oxidase system. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/308285

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Blanco, Marcos Luengo. “O papel do fator nuclear Kappa B (NF-k B) na expressão do gene CYBB do sistema NADPH oxidase humano: The role of nuclear factor k-B (NF-kB) CYBB gene expression activity of the human NADPH oxidase system.” 2008. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308285.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Blanco, Marcos Luengo. “O papel do fator nuclear Kappa B (NF-k B) na expressão do gene CYBB do sistema NADPH oxidase humano: The role of nuclear factor k-B (NF-kB) CYBB gene expression activity of the human NADPH oxidase system.” 2008. Web. 25 Feb 2021.

Vancouver:

Blanco ML. O papel do fator nuclear Kappa B (NF-k B) na expressão do gene CYBB do sistema NADPH oxidase humano: The role of nuclear factor k-B (NF-kB) CYBB gene expression activity of the human NADPH oxidase system. [Internet] [Thesis]. Universidade Estadual de Campinas; 2008. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308285.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Blanco ML. O papel do fator nuclear Kappa B (NF-k B) na expressão do gene CYBB do sistema NADPH oxidase humano: The role of nuclear factor k-B (NF-kB) CYBB gene expression activity of the human NADPH oxidase system. [Thesis]. Universidade Estadual de Campinas; 2008. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308285

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

4. Oliveira, Rômulo Tadeu Dias de. Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas: Identification of TH17 related cytokines in human atherosclerotic plaques.

Degree: 2010, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308991

► Abstract: The detection of activated T cells in human atherosclerotic plaques confirms their participation in the disease progression. In this scenario, IFN-? -producing T cells… (more)

▼ Abstract: The detection of activated T cells in human atherosclerotic plaques confirms their participation in the disease progression. In this scenario, IFN-? -producing T cells (TH1) are well recognized as having a pro-atherogenic role. Recently, a new subtype of IL-17-producing T helper (TH17) lineage of cells has been described and their association with inflammatory diseases, as well as their capacity to induce mediators frequently found in atherosclerotic lesions (IL-1? ? TNF-? ,?TGF-?,??CCL2 e MMP) suggest that they may have a role in atherosclerosis. The aim of this study was to investigate whether components of the immune response mediated by TH17 cells are present in human atherosclerotic lesions and their association with other immune mediators. For this purpose, 41 patients with critical stenosis of carotid submitted to endarterectomy were evaluated. Aortic fragments from organ donors were used as control. Carotid specimens were analyzed for mRNA and protein expression by real time RT-PCR and immunohistochemistry, respectively. In some experiments fragments of atherosclerotic plaques were incubated in vitro with IL-23, IL-2, LPS, PHA or ?CD3+?CD28 to further evaluate mRNA and protein expression. A low number of samples showed a small expression of IL-17 mRNA, while no staining for IL-17 was detected in any of the analyzed plaques. Up-regulation of IL-17 related genes (IL-23, RorC, CCR4, CCR4, IL-1?, TNF-?, MMP-9), as well as TH1 e TH2 (IFN-? e GATA-3), anti-inflammatory mediators (FoxP3, IL-10, EBI1 e IL-27R) and cytotoxic molecules (granzyme A, perforin e granulysin) mRNA expression were detected in patients plaques compared to control arteries. In addition, unstable plaques showed a high number of IL-23, IL-1?, IL-18, ICAM-1, IL-10 e TGF- ? ??granzyme B and perforin positive cells. Finally, the analysis of plaque derived cell suspensions revealed only 1% of IL-17+ cells, while in vitro stimulation resulted in IL-17 production only in the presence of anti-CD3+anti-CD28. Altogether our results showed components of TH17 response in association with mediators of TH1, TH2 and Treg response in human atherosclerotic lesions. Further studies are ongoing to elucidate the interaction, balance and collaborative potential among these effectors lineages in atherosclerosis control Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Blotta, Maria Heloisa de Souza Lima, 1953- (advisor), Blotta, Maria Heloisa Souza Lima (advisor), Menezes, Fábio Hüsemann, 1962- (coadvisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Ciências Médicas (nameofprogram), Burger, Eva (committee member), Junior, Carlos Vicente Serrano (committee member), Fernandes, Juliano de Lara (committee member), Mazon, Silvia de Barros (committee member).

Subjects/Keywords: Imunologia; Inflamação; Citocinas; Aterosclerose; Immunologya; Inflamation; Cytokine; Atherosclerosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, R. T. D. d. (2010). Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas: Identification of TH17 related cytokines in human atherosclerotic plaques. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/308991

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Rômulo Tadeu Dias de. “Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas: Identification of TH17 related cytokines in human atherosclerotic plaques.” 2010. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308991.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Rômulo Tadeu Dias de. “Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas: Identification of TH17 related cytokines in human atherosclerotic plaques.” 2010. Web. 25 Feb 2021.

Vancouver:

Oliveira RTDd. Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas: Identification of TH17 related cytokines in human atherosclerotic plaques. [Internet] [Thesis]. Universidade Estadual de Campinas; 2010. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308991.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira RTDd. Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas: Identification of TH17 related cytokines in human atherosclerotic plaques. [Thesis]. Universidade Estadual de Campinas; 2010. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/308991

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

5. Oliveira, Sara de Jesus. Estudo epidemiológico da paracoccidioidomicose na região de Campinas e avaliação da resposta imune celular na paracoccidioidomicose-infecção e nas diferentes formas da doença.

Degree: 2003, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310980

► Abstract: We studied the clinical-seroepidemiological characteristics of patients with paracoccidioidomycosis (PCM) attended at the University Hospital at UNICAMP (Campinas, São Paulo, Brazil). The study group… (more)

▼ Abstract: We studied the clinical-seroepidemiological characteristics of patients with paracoccidioidomycosis (PCM) attended at the University Hospital at UNICAMP (Campinas, São Paulo, Brazil). The study group consisted of 584 individuals (492 M, 92 F) with ages ranging from 5 to 87 years. The highest incidence of the disease occurred between the ages of 41 and 50 years for men and 11 and 40 for women. Rural activities were the principal occupation of 46% of the patients. The diagnosis was confirmed by histopathological examination and by demonstration of fungus in scrapings, secretions or in the sputum. Serological tests for PCM were positive in 80% of the 584 patients studied. The significant number of patients, including 33 children under 14 years old, is an indication of the fungus? presence in the area and indicates that this region is an important endemic area for paracoccidioidomycosis. Cellular immune response to Paracoccidiodes brasiliensis antigens (PbAg) was evaluated in patients with the juvenile (JF) and adult (AF) forms of paracoccidioidomycosis (PCM) as well as in a group of infected individuals living in the endemic area but without any clinical manifestation of the disease. The immune profile of this group of PCM-infected (PI) individuals was characterized by: 1) a positive skin test to P. brasiliensis antigen, 2) absence of specific antibodies, 3) a vigorous lymphoproliferative response to PbAg, and 4) a typical Th1 pattern of cytokines, with production of IFN-?, IL-2, IL-12 and TNF-? and basal levels of IL-4, IL-5 and IL-10. At the opposite end of the spectrum were the JF patients whose proliferative response to PbAg was significantly impaired and whose cytokine pattern was characteristically Th2, i.e., lower IFN-? TNF-? and IL-12 secretion and significantly higher levels of IL-4 and IL-5. These profiles are compatible with forms of higher and lower resistance, respectively. Intermediate immune responses were observed in AF patients, whose specific lymphoproliferative response was lower than in the PI group but higher than in the JF patients. The secretion of IFN-? and IL-10 did not differ from the JF group, although IL-4 and IL-5 levels were significantly lower, and TNF-? and IL-12 higher. Since AF patients are able to control fungal dissemination for decades, they can be considered more resistant than JF patients, who manifest the disease soon after infection Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Camargo, Irineu Jose Barsanti de, 1942- (advisor), Blotta, Maria Heloisa Souza Lima (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Ciências Médicas (nameofprogram), Santos, Leonilda M.B. (committee member), Ferro, Maria Cecília (committee member), Branchini, Maria Luiza Moretti (committee member).

Subjects/Keywords: Epidemiologia; Linfócitos T; Interleucina 2; Paracoccidioides; Epidemiology; T-Lymphocytes; Interleukin 2; Paracoccidioides brasiliensis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, S. d. J. (2003). Estudo epidemiológico da paracoccidioidomicose na região de Campinas e avaliação da resposta imune celular na paracoccidioidomicose-infecção e nas diferentes formas da doença. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/310980

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Sara de Jesus. “Estudo epidemiológico da paracoccidioidomicose na região de Campinas e avaliação da resposta imune celular na paracoccidioidomicose-infecção e nas diferentes formas da doença.” 2003. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310980.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Sara de Jesus. “Estudo epidemiológico da paracoccidioidomicose na região de Campinas e avaliação da resposta imune celular na paracoccidioidomicose-infecção e nas diferentes formas da doença.” 2003. Web. 25 Feb 2021.

Vancouver:

Oliveira SdJ. Estudo epidemiológico da paracoccidioidomicose na região de Campinas e avaliação da resposta imune celular na paracoccidioidomicose-infecção e nas diferentes formas da doença. [Internet] [Thesis]. Universidade Estadual de Campinas; 2003. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310980.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira SdJ. Estudo epidemiológico da paracoccidioidomicose na região de Campinas e avaliação da resposta imune celular na paracoccidioidomicose-infecção e nas diferentes formas da doença. [Thesis]. Universidade Estadual de Campinas; 2003. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310980

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

6. Soares, Lanny Cristina Burlandy. Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose: Evaluation of cytotoxic molecules production by peripheral blood lymphocytes (CD8+ and CD4+) of patients with paracoccidioidomycosis.

Degree: 2008, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310976

► Abstract: CD8+ T cells play a pivotal role in host defense against diseases caused by intracellular pathogens such as tuberculosis, cryptococcosis and listeriosis. In a… (more)

▼ Abstract: CD8+ T cells play a pivotal role in host defense against diseases caused by intracellular pathogens such as tuberculosis, cryptococcosis and listeriosis. In a previous study we verified an increased number of T CD8+ cells in bronchoalveolar lavage of patients with pulmonary paracoccidioidomycosis (PCM), suggesting a role for them in the local immune response. CD8+ T cells effector functions include cytokines production, mainly IFN-? and cytotoxic activity. The aims of this study were to verify the activation state as well as the production of cytotoxic molecules by peripheral blood lymphocytes (CD8+ and CD4+) from patients with PCM, individuals with PCM-infection and controls, both ex-vivo and in vitro after stimulation with P. brasiliensis yeast cells. The expression of activation and cytotoxic molecules was evaluated by flow cytometry. The ex-vivo analysis showed that, in general, the patients presented a lower frequency of granzime A, B and perforin-positive cells as compared to PCM-infection individuals. P. brasiliensis yeast cells stimulation led to a discrete increase in CD69+ cells and a reduction in cytotoxic granules expression in all groups. The addition of IL-15 to the cultures induced an increase in the frequency of CD69+ cells only in individuals with PCM-infection and controls. Differently, CD8+ and CD4+ T cells from PCM patients were activated only in the absence of fungal cells. The effect of IL-15 in granzyme A and B expression was low but a higher frequency of CD8+perforin+ was detected in individuals with PCM-infection than in patients with PCM. IL-15Ra expression was lower in CD4+ T cells from patients in relation to individuals with PCM-infection and controls. The detection of granulysin levels by ELISA showed lower levels in PCM patients than in individuals with PCM-infection and controls. Moreover, a tendency to a rise in granulysin levels was observed after antifungal therapy. Altogether the results showed that lymphocytes from PCM patients are poorly activated, express low levels of IL-15Ra and produce basal levels of cytotoxic granules (granzyme A, B, perforin and granulysin). These findings, in addition to other mechanisms that impair cellular immunity, may account to defective cytotoxic activity and consequently low capacity to kill the fungus Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Blotta, Maria Heloisa de Souza Lima, 1953- (advisor), Blotta, Maria Heloisa Souza Lima (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), Programa de Pós-Graduação em Ciências Médicas (nameofprogram), Soares, Angela Maria Victoriano de Campos (committee member), Burger, Eva (committee member), Verinaud, Liana Maria Cardoso (committee member), Trabasso, Plinio (committee member).

Subjects/Keywords: Paracoccidioidomicose; Celulas T; Citometria de fluxo; Interleucina - 15; Paracoccidioidomycosis; T cells; Flow cytometry; Interleukin - 15

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Soares, L. C. B. (2008). Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose: Evaluation of cytotoxic molecules production by peripheral blood lymphocytes (CD8+ and CD4+) of patients with paracoccidioidomycosis. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/310976

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Soares, Lanny Cristina Burlandy. “Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose: Evaluation of cytotoxic molecules production by peripheral blood lymphocytes (CD8+ and CD4+) of patients with paracoccidioidomycosis.” 2008. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310976.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Soares, Lanny Cristina Burlandy. “Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose: Evaluation of cytotoxic molecules production by peripheral blood lymphocytes (CD8+ and CD4+) of patients with paracoccidioidomycosis.” 2008. Web. 25 Feb 2021.

Vancouver:

Soares LCB. Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose: Evaluation of cytotoxic molecules production by peripheral blood lymphocytes (CD8+ and CD4+) of patients with paracoccidioidomycosis. [Internet] [Thesis]. Universidade Estadual de Campinas; 2008. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310976.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soares LCB. Avaliação do estado de ativação e da produção de moleculas citotoxicas por linfocitos (CD4+ e CD8+) do sangue periferico de pacientes com paracoccidioidomicose: Evaluation of cytotoxic molecules production by peripheral blood lymphocytes (CD8+ and CD4+) of patients with paracoccidioidomycosis. [Thesis]. Universidade Estadual de Campinas; 2008. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/310976

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

7. Mamoni, Ronei Luciano. Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença.

Degree: 2005, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/313406

► Abstract: Paracoccidioidomycosis (PCM) presents three major forms: PCM-infection (PI); adult form (AF) and juvenile form (JF). The aim of this study was to compare the… (more)

▼ Abstract: Paracoccidioidomycosis (PCM) presents three major forms: PCM-infection (PI); adult form (AF) and juvenile form (JF). The aim of this study was to compare the immunological response in these groups through the analysis of the kinectics of mRNA expression of some cytokines and chemokines (IL-2, IL-4, IL-5, IL-6, IL-lO, IFN-y, TGF p, TNF-a, CXCLS, CXCL9 and CXCLlO) by RT-PCR, as well as, to characterize the phenotype of the cytokines producing cells by flow cytometry. The results showed that PI individuaIs express earlier and higher levels of mRNA for IFN-y, TNF-a, CXCL9 and CXCLIO when compared to JF patients and similar levels of CXCLlO and IFN-y and higher levels of CXCL9 and TNF -a when compared to AF ones. On the other hand, the mRNA expression ofTh2 cytokines (IL-4, IL-IO, IL-5 and TGF-P) was higher and earlier in JF and AF groups when compared to PI individuaIs. In relation to protein expression, we observed that PI group presents a higher percentage of cells producing IFN-y, TNF-a, IL-2, CXCL9 and CXCLIO when compared to both groups ofpatients. The production ofIFN-y was predominantIy done by CD3+CDS+ T cells, whereas IL-2 and TNF-a production was higher in CD3+CD4+ cells. Monocytes ofPI individuaIs also presented higher expression of CDSO and lower expression of CDS6 and CD54 when compared to JF and AF patients, and higher expression of HLA-DR and CDIIa only when compared to JF patients. These results indicate that the differential kinetic of mRNA expression of ThI or Th2 cytokines observed in the different forms of PCM may modulate the immunological response resulting in the different outcomes observed in this disease Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Blotta, Maria Heloisa de Souza Lima, 1953- (advisor), Blotta, Maria Heloisa Souza Lima (advisor), Universidade Estadual de Campinas. Faculdade de Ciências Médicas (institution), -Yasuda, Maria Aparecida Shikanai (committee member), Calich, Vera Lucia Garcia (committee member), Verinaud, Liana Maria Cardoso (committee member), Trabasso, Plinio (committee member).

Subjects/Keywords: Paracoccidioidomicose; Citocinas; Quimiocinas; Imunologia celular

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mamoni, R. L. (2005). Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/313406

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mamoni, Ronei Luciano. “Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença.” 2005. Thesis, Universidade Estadual de Campinas. Accessed February 25, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313406.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mamoni, Ronei Luciano. “Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença.” 2005. Web. 25 Feb 2021.

Vancouver:

Mamoni RL. Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença. [Internet] [Thesis]. Universidade Estadual de Campinas; 2005. [cited 2021 Feb 25]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/313406.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mamoni RL. Caracterização da paracoccidioidomicose-infecção como a forma de resistencia da paracoccidioidomicose humana : comparação com as formas juvenil e adulta da doença. [Thesis]. Universidade Estadual de Campinas; 2005. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/313406

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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