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You searched for +publisher:"Universidade Estadual de Campinas" +contributor:("Ballus, Cristiano Augusto, 1985-"). One record found.

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Universidade Estadual de Campinas

1. Paludo, Michelly Cristiane, 1986-. Brazilian jabuticabas   : phenolic characterization, capacity of deactivation of reactive species of oxygen and nitrogen and antiproliferative potential in prostate and breast tumor cells  : Jabuticabas brasileiras   : caracterização fenólica, capacidade de desativação de espécies reativas de oxigênio e nitrogênio e potencial antiproliferativo sob células tumorais de próstata e mama  .

Degree: 2018, Universidade Estadual de Campinas

Abstract: Jabuticaba is a Brazilian fruit, rich in phenolic compounds, specially anthocyanins. These compounds present higher antioxidant capacity and are related to the prevention of many chronic degenerative diseases. Thus, the present study aimed optimizing the extraction process of total phenolic compounds (TPC) in jabuticaba seeds, as well as total monomeric anthocyanins (TMA) in jabuticaba skins, and its characterization. It was also evaluated the potential of jabuticaba seeds and skins extracts, from five jabuticaba varieties, in the deactivation capacity of reactive oxygen species (ROS) and reactive nitrogen species (RNS), as well as the determination of the antiproliferative potential in prostate and breast tumor cells. The optimization of the extraction of TPC from seeds and TMA from skins was successfully performed and the best conditions were ethanol:water (60:40 v/v) for TPC from seeds and methanol:water:acetic acid (80:20:0,5 v/v/v) for TMA from skins; for both compounds classes the total extraction time was 30 min. The capacity of seeds and skins extracts on deactivation capacity of reactive oxygen species (ROS) and reactive nitrogen species (RNS) was also evaluated. Both extracts can be considered significant sources of exogenous antioxidants: Sabará skins (SPF) deactivated the HOCl with IC50 9.24 µg.mL-1; Paulista seeds (PF) deactivated the O2¿? with IC50 16.15 µg.mL-1; Coroada seeds (CFP) deactivated the ONOO- in the absence of NaHCO3 with IC50 3.84 µg.mL-1; CFP skins deactivated the ONOO- in the presence of NaHCO3 with IC50 5.88 µg.mL-1; SFP skins deactivated the ROO¿ (ORAC) in 918.16 ?mol TE g-1 and the Sabará seeds deactivated the H2O2 with 49.11% of inhibition in the concentration of 125 µg of extract.mL-1. To complement this study, it was performed the mitochondrial activity assay (MTT) in breast tumor cells (MDA-MB-231) and in prostate tumor cells (DU-145); the trial results allowed to conclude that the skin extracts present an excellent action against these tumor cells. PFP skins extract inhibited the MDA-MB-231 cellular growth with IC50 25.86 µg.mL-1 of extract. The identification and quantification of anthocyanic and non-anthocyanic phenolic compounds were performed by high-performance liquid chromatography coupled to mass spectrometry. The main anthocyanins identified in the samples were cyanidig-3-glycoside and delphynidin-3-glycoside. It was also found three myricetin derivatives; 14 quercetin derivatives, including free-quercetin; 13 elagic acid derivatives, including free-elagic acid; and, 4 methyl elagic acid derivatives. Thus, it was possible to characterize the phenolic compounds profile of the jabuticaba varieties that were not been studied yet. With the above information it is clear that the extraction method optimized here was extremely fast, simple, inexpensive and with adequate extraction performance. The analyzed extracts presented high antioxidant potential and efficiently reduced the proliferation of DU-145 and MDA-MB-231 cells, demonstrating that jabuticaba is extremely… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Godoy, Helena Teixeira, 1957- (advisor), Ballus, Cristiano Augusto, 1985- (coadvisor), Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos (institution), Programa de Pós-Graduação em Ciência de Alimentos (nameofprogram), Oliveira, Silvia Borges Pimentel de (committee member), Nixdorf, Suzana Lucy (committee member), Prado, Marcelo Alexandre (committee member).

Subjects/Keywords: Apoptose; Antioxidantes; Estresse oxidativo; Antocianinas; Antioxidants; Oxidative stress; Anthocyanins

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APA (6th Edition):

Paludo, Michelly Cristiane, 1. (2018). Brazilian jabuticabas   : phenolic characterization, capacity of deactivation of reactive species of oxygen and nitrogen and antiproliferative potential in prostate and breast tumor cells  : Jabuticabas brasileiras   : caracterização fenólica, capacidade de desativação de espécies reativas de oxigênio e nitrogênio e potencial antiproliferativo sob células tumorais de próstata e mama  . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/334059

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Paludo, Michelly Cristiane, 1986-. “Brazilian jabuticabas   : phenolic characterization, capacity of deactivation of reactive species of oxygen and nitrogen and antiproliferative potential in prostate and breast tumor cells  : Jabuticabas brasileiras   : caracterização fenólica, capacidade de desativação de espécies reativas de oxigênio e nitrogênio e potencial antiproliferativo sob células tumorais de próstata e mama  .” 2018. Thesis, Universidade Estadual de Campinas. Accessed November 29, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/334059.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Paludo, Michelly Cristiane, 1986-. “Brazilian jabuticabas   : phenolic characterization, capacity of deactivation of reactive species of oxygen and nitrogen and antiproliferative potential in prostate and breast tumor cells  : Jabuticabas brasileiras   : caracterização fenólica, capacidade de desativação de espécies reativas de oxigênio e nitrogênio e potencial antiproliferativo sob células tumorais de próstata e mama  .” 2018. Web. 29 Nov 2020.

Vancouver:

Paludo, Michelly Cristiane 1. Brazilian jabuticabas   : phenolic characterization, capacity of deactivation of reactive species of oxygen and nitrogen and antiproliferative potential in prostate and breast tumor cells  : Jabuticabas brasileiras   : caracterização fenólica, capacidade de desativação de espécies reativas de oxigênio e nitrogênio e potencial antiproliferativo sob células tumorais de próstata e mama  . [Internet] [Thesis]. Universidade Estadual de Campinas; 2018. [cited 2020 Nov 29]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/334059.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paludo, Michelly Cristiane 1. Brazilian jabuticabas   : phenolic characterization, capacity of deactivation of reactive species of oxygen and nitrogen and antiproliferative potential in prostate and breast tumor cells  : Jabuticabas brasileiras   : caracterização fenólica, capacidade de desativação de espécies reativas de oxigênio e nitrogênio e potencial antiproliferativo sob células tumorais de próstata e mama  . [Thesis]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/334059

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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