+publisher:"Universidade Estadual de Campinas" +contributor:("Arruda, Eliane Pintor de")

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Universidade Estadual de Campinas

1. Quast, Mônica Paiva, 1977-. Investigação de limites específicos em Corbula (Corbulidae: Bivalvia) do Sudeste e Sul do Brasil, com base em marcadores moleculares: Species boundaries in Corbula (Corbulidae: Bivalvia) from South-Southeastern Brazil based on molecular markers.

Degree: 2013, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/315774

Abstract: Species are fundamental unities in many biological studies and, being so, their identification is essential for researches in many different fields. This task, however, is complicated by badly defined interspecies boundaries, especially in the sea, where cryptic species are quite common. Species delimitation has been receiving much attention, and molecular techniques have been proved of great value to the matter. Corbula (Bivalvia, Corbulidae) is frequent and ecologically important genus in benthic marine communities. Nevertheless, its taxonomy is confusing, in part due to a plastic shell, which makes it difficult to establish species boundaries. This study aimed to analyze the COI and 16S sequences of six morphologically identified Corbula species occurring off the South-Southeastern Brazilian coast. Being a mainly sublittoral genus, most of the analyzed material had been previously sampled, fixed in formalin and preserved in alcohol. Hence, initially specific protocols for DNA extraction and PCR were developed. Better results were obtained with an extraction protocol combining organic extraction and silica adsorption. The nested PCR yielded more product than the direct PCR. Delimitation analyses were conducted with four different methods: two tree based (GMYC and Brownie) and two non-tree based (4x rule and ABGD). Different methods and markers produced different delimitations, but the combined evidence supports the morphological delimitation of three species: Corbula caribaea, Corbula tryoni and Corbula lyoni. Individuals assigned to Corbula patagonica were separated into two molecular species. Only one individual of Corbula aequivalvis was analyzed and it was distinguished from other species. One individual assigned to Corbula sp1 could not be distinguish from C. caribaea Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Amaral, Antonia Cecília Zacagnini, 1948- (advisor), Solferini, Vera Nisaka, 1957- (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Ecologia (nameofprogram), Arruda, Eliane Pintor de (committee member), José, Juliana (committee member), Marian, José Eduardo Amoroso Rodriguez (committee member), Passos, Flávio Dias (committee member).

Subjects/Keywords: Corbulidae; Delimitação de espécies; Material formolizado; Corbulidae; Molecular taxonomy; Species delimitation; Formalin-fixed specimens

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APA (6^th Edition):

Quast, Mônica Paiva, 1. (2013). Investigação de limites específicos em Corbula (Corbulidae: Bivalvia) do Sudeste e Sul do Brasil, com base em marcadores moleculares: Species boundaries in Corbula (Corbulidae: Bivalvia) from South-Southeastern Brazil based on molecular markers. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/315774

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Quast, Mônica Paiva, 1977-. “Investigação de limites específicos em Corbula (Corbulidae: Bivalvia) do Sudeste e Sul do Brasil, com base em marcadores moleculares: Species boundaries in Corbula (Corbulidae: Bivalvia) from South-Southeastern Brazil based on molecular markers.” 2013. Thesis, Universidade Estadual de Campinas. Accessed March 07, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315774.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Quast, Mônica Paiva 1. Investigação de limites específicos em Corbula (Corbulidae: Bivalvia) do Sudeste e Sul do Brasil, com base em marcadores moleculares: Species boundaries in Corbula (Corbulidae: Bivalvia) from South-Southeastern Brazil based on molecular markers. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2021 Mar 07]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/315774.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Quast, Mônica Paiva 1. Investigação de limites específicos em Corbula (Corbulidae: Bivalvia) do Sudeste e Sul do Brasil, com base em marcadores moleculares: Species boundaries in Corbula (Corbulidae: Bivalvia) from South-Southeastern Brazil based on molecular markers. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/315774

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Machado, Fabrizio Marcondes, 1984-. Desvendando a diversidade dos Anomalodesmata (Mollusca: Bivalvia) : uma abordagem morfológica e filogenética: Unravelling the diversity of Anomalodesmata (Mollusca: Bivalvia) : a morphological and phylogenetic approach.

Degree: 2018, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333877

Abstract: Considered a monophyletic group, the Anomalodesmata Dall, 1889 currently have more than 800 species, bearing some of the rarest, bizarre and specialized marine bivalves. This rarity combined with the scarcity of detailed anatomical information about their species has always been obstacles to the understanding of their internal relationships, consequently, also resulting in a low representativeness of taxa in phylogenetic reconstructions. In order to fill some of the main gaps in the knowledge of Anomalodesmata, the present thesis proposed three specific objectives, by using different tools, to expand, update and present new insights about the evolutionary, morphological and taxonomic knowledge of this important group of bivalves. The first objective took place through field collections in the Araçá Bay, north coast of the State of São Paulo, where it was possible to observe and to describe in detail the functional morphology and behaviour of Cardiomya cleryana, bringing new ideas about the carnivorous bivalves as a whole. The second goal was through the access to the malacological collection of the Museum of Comparative Zoology - Harvard University, where most of the lots deposited in this institution were reviewed, generating new conchological and anatomical data for the elaboration of a morphological analysis, generating a new phylogeny for the Anomalodesmata. Finally, the third objective was to increase the anatomical knowledge of anomalodesmatans by using X-ray microtomography, culminating in the first contrast protocols for marine bivalves, comparisons between different invasive techniques, and the first anatomical descriptions of bivalves based exclusively on tomographic images and 3D reconstructions Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Passos, Flávio Dias, 1971- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Animal (nameofprogram), Amaral, Antonia Cecília Zacagnini (committee member), Arruda, Eliane Pintor de (committee member), Oliveira, Cléo Dilnei de Castro (committee member), Garraffoni, André Rinaldo Senna (committee member).

Subjects/Keywords: Bivalve; Microtomografia por raio-X; Filogenia; Anatomia; Molusco; Bivalves; X-ray microtomography; Phylogeny; Anatomy; Mollusks

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Machado, Fabrizio Marcondes, 1. (2018). Desvendando a diversidade dos Anomalodesmata (Mollusca: Bivalvia) : uma abordagem morfológica e filogenética: Unravelling the diversity of Anomalodesmata (Mollusca: Bivalvia) : a morphological and phylogenetic approach. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/333877

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Machado, Fabrizio Marcondes, 1984-. “Desvendando a diversidade dos Anomalodesmata (Mollusca: Bivalvia) : uma abordagem morfológica e filogenética: Unravelling the diversity of Anomalodesmata (Mollusca: Bivalvia) : a morphological and phylogenetic approach.” 2018. Thesis, Universidade Estadual de Campinas. Accessed March 07, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/333877.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Machado, Fabrizio Marcondes 1. Desvendando a diversidade dos Anomalodesmata (Mollusca: Bivalvia) : uma abordagem morfológica e filogenética: Unravelling the diversity of Anomalodesmata (Mollusca: Bivalvia) : a morphological and phylogenetic approach. [Internet] [Thesis]. Universidade Estadual de Campinas; 2018. [cited 2021 Mar 07]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333877.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Machado, Fabrizio Marcondes 1. Desvendando a diversidade dos Anomalodesmata (Mollusca: Bivalvia) : uma abordagem morfológica e filogenética: Unravelling the diversity of Anomalodesmata (Mollusca: Bivalvia) : a morphological and phylogenetic approach. [Thesis]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333877

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

3. Introíni, Gisele Orlandi. Analise ultra-estrutural de espermatozoides de especies de bivalves marinhos: Spermatozoan ultrastructural analyses of marine bivalve species.

Degree: 2009, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317967

Abstract: The ultrastructure of bivalve spermatozoa can be species-specific and often provide valuable taxonomic traits for systematic reviews and phylogenetic reconstructions. Transmission and scanning electron microscopy was an efficient tool to differentiate the spermatozoan structure of species sharing many conchological characters, which could lead to equivocal identification. We analyzed sperm ultrastructural features that were useful for taxonomic issues with regard to Isognomon bicolor and Isognomon alatus (Isognomonidae); Macoma constricta, Macoma biota and Tellina lineata (Tellinidae); Donax hanleyanus and Donax gemmula (Donacidae). The data revealed that the bivalve species studied here, except T. lineata, produce primitive-type spermatozoa, typical of marine invertebrates in which eggs are fertilized in the surrounding water. We, also, compared the ultrastructure of spermatozoa from the bivalves Anomalocardia brasiliana and Tivela mactroides (Veneridae). The spermatozoa of both species contain a curved nucleus with a short cone-shaped acrosome. There were six mitochondria and glycogen clusters in the middle piece of the T. mactroides sperm cell. Possibly, the glycogen clusters and the higher mitochondria number correspond to an adaptive advantage in turbulent waters. Increasing the sperm life expectance also increases the odds of finding the eggs and accomplishing fertilization. Oysters of the genus Crassostrea and mussels of the genus Mytella, commercially important in Brazil, were also investigated in this thesis. The sperm ultrastructure of the specimens described herein and molecular studies, confirm the presence of Crassostrea brasiliana (Ostreidae) in Cananéia. Spermatozoa of mussel specimens of the genus Mytella, from two populations living in distinct habitats (muddy-sand sediment and rocky shores), were examined by transmission electron microscopy. The spermatozoa of all specimens shared the same ultrastructural pattern suggesting that the analyzed specimens, which live in distinct habitats, indeed belong to a same species, which was identified as M. charruana (Mytilidae) based on conchological analysis. The presence of an axial rod in sperm cells upholds the inclusion of M. charruana in the subfamily Mytilinae. In conclusion the electron microscopy was an efficient method to solve some particular taxonomic issues. The present spermatozoan ultrastructural studies have provided new and welcome data about the families Isognomonidae, Veneridae, Tellinidae, Donacidae, Ostreidae and Mytilidae. The results showed in this thesis could be valuable in future phylogenetic analyses. Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Recco-Pimentel, Shirlei Maria, 1954- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Celular e Estrutural (nameofprogram), Kawano, Toshie (committee member), Arruda, Eliane Pintor de (committee member), Matos, Edilson Rodrigues (committee member), Joazeiro, Paulo Pinto (committee member).

Subjects/Keywords: Ultraestrutura (Biologia); Espermatozóides; Bivalve; Tellinidae; Veneridae; Ultrastructure (Biology); Spermatozoa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Introíni, G. O. (2009). Analise ultra-estrutural de espermatozoides de especies de bivalves marinhos: Spermatozoan ultrastructural analyses of marine bivalve species. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317967

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Introíni, Gisele Orlandi. “Analise ultra-estrutural de espermatozoides de especies de bivalves marinhos: Spermatozoan ultrastructural analyses of marine bivalve species.” 2009. Thesis, Universidade Estadual de Campinas. Accessed March 07, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317967.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Introíni, Gisele Orlandi. “Analise ultra-estrutural de espermatozoides de especies de bivalves marinhos: Spermatozoan ultrastructural analyses of marine bivalve species.” 2009. Web. 07 Mar 2021.

Vancouver:

Introíni GO. Analise ultra-estrutural de espermatozoides de especies de bivalves marinhos: Spermatozoan ultrastructural analyses of marine bivalve species. [Internet] [Thesis]. Universidade Estadual de Campinas; 2009. [cited 2021 Mar 07]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317967.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Introíni GO. Analise ultra-estrutural de espermatozoides de especies de bivalves marinhos: Spermatozoan ultrastructural analyses of marine bivalve species. [Thesis]. Universidade Estadual de Campinas; 2009. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317967

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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