+publisher:"Universidade Estadual de Campinas" +contributor:("Andreote, Fernando Dini")

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Universidade Estadual de Campinas

1. Domingos, Daniela Ferreira, 1984-. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

► Abstract: Mangrove is an environment rich in microbial diversity, but little has been reported about it in Brazil, making the knowledge and exploitation of new… (more)

▼ Abstract: Mangrove is an environment rich in microbial diversity, but little has been reported about it in Brazil, making the knowledge and exploitation of new microorganisms in mangroves an imperative issue. The prospection of microbial diversity in underexplored environments, such as mangroves, increses possibility of success in looking for new bioactive molecules, contributing to a more sustentable economy and environment. Although the cultivation techniques have been improved, allowing an increased number of yet uncultivated microorganisms to be able recuperated in vitro, our knowledge about their ecology is still insufficient to cultivate the majority of them. Therefore, the metagenomic library have emerged as a powerful tool to more widely access the overall microbial diversity in an environment, enabling the functional analyses of genes and the discovery of new bioactive compounds, mainly uncultured-microorganisms. The aim of the current work was the prospection of biosurfactant from mangrove microbiota through a polifasica approach. For the independent-cultivation approach, a metagenomic library of high molecular weight was constructed from mangrove sediment contaminated with oil. The clones were subjected to functional and molecular screening to identify biosurfactant activity. Three clones with the potential to procuce biosurfactant were selected, and the fosmid sequencing for genetic chatacterization of the insert was carried out. Although the clones showed the ability to reduce the surface tension, genes that may involved in biosurfactant synthesis were not identified. The results showed that using the functional metagenomic to explore metabolic pathways of microbial communities can have great limitation when in the prospection of biosurctants when the operon are larger then 30-40 kb and the genome of wild bacteria contains sparse regulation elements. For the dependent-cultivation approach, two strains that produce biosurfactant were selected: Bacillus safensis CCMA-560 and Gordonia sp. CCMA-559. They were isolated and tested in previous study. The biosurfactant production was opmized through the Placktt-Burman design and the Central Composite Design. The pumilacidin produced by B. safensis CCMA-560 was chemically characterized, and the metabolic pathway that is responsible for its production was genetically characterized. This work was the first report the physiologic, genetic, and chemical analysis on the biosurfactant production by the B. safensis strain Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Melo, Itamar Soares de (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Andreote, Fernando Dini (committee member), Araújo, Welington Luiz de (committee member), Vicentini, Renato (committee member), Duarte, Marta Cristina Teixeira (committee member).

Subjects/Keywords: Biossurfactantes; Metagenômica; Bacillus safensis; Gordonia (Bactéria); Biosurfactants; Metagenomics; Bacillus safensis; Gordonia (Bacteria)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Domingos, Daniela Ferreira, 1. (2014). Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Domingos, Daniela Ferreira, 1984-. “Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota.” 2014. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Domingos, Daniela Ferreira 1. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Domingos, Daniela Ferreira 1. Prospecção de biossurfactantes a partir de microbiota de manguezais = Prospection of biosurfactant from mangrove macrobiota: Prospection of biosurfactant from mangrove macrobiota. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317333

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Lacerda Júnior, Gileno Vieira, 1987-. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.

Degree: 2017, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987

► Abstract: Caatinga is an exclusively Brazilian biome, poorly studied and preserved, with an exuberant and exclusive biological diversity. This ecosystem consists of a dry tropical… (more)

▼ Abstract: Caatinga is an exclusively Brazilian biome, poorly studied and preserved, with an exuberant and exclusive biological diversity. This ecosystem consists of a dry tropical forest adapted to severe climatic conditions, including high temperatures and solar irradiation, soil acidity, and water deficit caused by the seasonal rainfall regime. Therefore, it is expected to harbor a soil microbiota carrying unique and versatile enzymatic arsenals with a potential biotechnological application. Resident soil microorganisms are capable of decomposing lignin (second most abundant biopolymer of the biosphere) using a set of ligninolytic enzymes which can also be applied in the treatment of effluents, pulp and paper industry, biofuels productions, among other industrial processes. Considering that about 99% of soil microorganisms cannot be isolated by conventional culture methods, molecular approaches based on the direct extraction of environmental genetic material, such as "metagenomics", are needed to provide more precise information about the diversity and biotechnological potential of the native microbial community. Thus, this study uses an integrated approach of functional (based on biological activity and sequence similarity) and descriptive metagenomics, with the aim of identifying new lignocellulolytic enzymes, in addition to analyzing the impact of seasonality and agricultural practices (irrigation/fertilization) in the community structure and functional responses of the Caatinga soil microbiome. The functional screening based on the phenotypic expression of 40,000 metagenomic clones did not show any ligninolytic activity. However, the molecular screening by PCR allowed the identification of a bacterial Laccase-like Multicopper oxidase, affiliated with the genus Mesorhizobium. The large-scale sequencing of a soil metagenomic library and subsequent in silico analysis revealed a large enzymatic repertoire related to the lignocellulosic degradation carried by different bacterial groups with potential application in the biofuels production as well as other environmental processes. In an ecological approach, it was observed the enrichment of the phyla Actinobacteria and Proteobacteria in the drought and rainy seasonal period, respectively. The data suggest that microorganisms thriving on native soils of the dry period are specialized in monosaccharides and disaccharides utilization, as well as osmoprotective sugars such as trehalose. In contrast, the soil microbiome affected by land use was mainly driven by chemical factors, with the enrichment of the Acidobacteria phylum and genes related to nitrification and denitrification processes, and to oxidative stress. These results suggest that the nutrients input and the breakdown of the natural cycles of the water regime may impact the biogeochemical cycles, affecting the natural functionality of the Caatinga semiarid soils Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Souza, Anete Pereira de (committee member), Vicentini, Renato (committee member), Mendes, Rodrigo (committee member), Andreote, Fernando Dini (committee member).

Subjects/Keywords: Caatinga; Micro-organismos do solo; Metagenômica; Bioprospecção; Enzimas ligninolíticas; Caatinga; Soil microbiology; Metagenomics; Bioprospecting; Ligninolytic enzymes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lacerda Júnior, Gileno Vieira, 1. (2017). Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lacerda Júnior, Gileno Vieira, 1987-. “Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation.” 2017. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Lacerda Júnior, Gileno Vieira 1. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. [Internet] [Thesis]. Universidade Estadual de Campinas; 2017. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lacerda Júnior, Gileno Vieira 1. Caracterização do microbioma de solos semiáridos da Caatinga e seu potencial para a degradação de lignocelulose: Characterization of the microbiome of Caatinga semiarid soils and its potential for lignocellulose degradation. [Thesis]. Universidade Estadual de Campinas; 2017. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331987

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

3. Sierra Garcia, Isabel Natalia, 1984-. Investigation of the microbiota and processes involved in petroleum degradation in oil reservoirs : Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo: Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333609

► Abstract: Petroleum reservoirs are deep subsurface environments of global importance as energy resources and in biogeochemical cycles. Microorganisms living in petroleum reservoirs are able to… (more)

▼ Abstract: Petroleum reservoirs are deep subsurface environments of global importance as energy resources and in biogeochemical cycles. Microorganisms living in petroleum reservoirs are able to use petroleum hydrocarbons as carbon sources, leading to biodegradation and negatively affecting the quality and economic value of oil. Previous studies suggest that anaerobic microorganisms are responsible for in-reservoir oil biodegradation. However, little is known about the actual microbial diversity and metabolic processes involved in biodegradation, mainly because of the difficulty in recovering the complex microbial community present in such extreme environments. This work aimed to characterize the microbiota of two petroleum reservoir samples using culture-independent approaches and assess the anaerobic hydrocarbon degradation potential of the indigenous microorganisms under different physicochemical conditions. The study revealed clear differences in the phylogenetic diversity between the degraded (BA-1) and non-degraded (BA-2) oil samples based on the analysis of bacterial and archaeal 16S rRNA gene clone libraries. The taxonomic and functional differences were also evident in the metagenomic profiles of the two petroleum reservoir samples. The metagenome from the BA-1 sample showed high abundance of genes related with the anaerobic hydrocarbon degradation, methanogenesis and syntrophic associations, while the BA-2 sample showed abundance of aerobic hydrocarbon degradation genes. Correspondingly, a microbial anaerobic active consortium was successfully cultivated from the BA-1 oil sample capable of metabolizing the crude oil components to methane. Higher rates of methane production were observed at the lowest salinity and at the in situ reservoir temperature. Together, the highest salt concentration (120 gl-1) and temperature (70 ºC) tested showed to inhibit the methanogenic activity of the consortium. Data obtained demonstrate that crude oil can harbour an indigenous microbiota with the ability to degrade petroleum hydrocarbons predominantly via methanogenic metabolism. Results gathered herein, and the ongoing work, will help us to understand the potential roles of microbes in hydrocarbon biodegradation and how such organisms respond to the environmental conditions in the petroliferous subsurface. In addition, the unveiled potential of the BA-1 microbiota to convert oil components to methane has an important contribution to the petroleum industry, as methane might be the future for energy production from deep or depleted reservoirs Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Araujo, Wellington Luiz de (committee member), Nakayama, Cristina Rossi (committee member), Andreote, Fernando Dini (committee member), Vicentini, Renato (committee member).

Subjects/Keywords: Petróleo; Biodegradação; Anaerobiose; Diversidade microbiana; Metagenômica; Petroleum; Biodegradation; Anaerobiosis; Microbial diversity; Metagenomics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sierra Garcia, Isabel Natalia, 1. (2016). Investigation of the microbiota and processes involved in petroleum degradation in oil reservoirs : Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo: Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/333609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sierra Garcia, Isabel Natalia, 1984-. “Investigation of the microbiota and processes involved in petroleum degradation in oil reservoirs : Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo: Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/333609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Sierra Garcia, Isabel Natalia 1. Investigation of the microbiota and processes involved in petroleum degradation in oil reservoirs : Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo: Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sierra Garcia, Isabel Natalia 1. Investigation of the microbiota and processes involved in petroleum degradation in oil reservoirs : Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo: Investigação da microbiota e dos processos envolvidos na degradação de óleo em reservatórios de petróleo. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/333609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

4. Tomazetto, Geizecler, 1979-. Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2: Diversity analysis and bioprospection of microbial metagenome in a H2-producing biogas fermenter.

Degree: 2013, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317324

► Abstract: Hydrogen appears to be the most promising candidate for the replacement of fossil fuel due to its potentially higher efficiency of conversion to usable… (more)

▼ Abstract: Hydrogen appears to be the most promising candidate for the replacement of fossil fuel due to its potentially higher efficiency of conversion to usable power and no toxic emission production. The production of hydrogen from organic wastes is performed through the anaerobic digestion, making it an environmentally friendly alternative for satisfying future hydrogen demands. Nonetheless, the microorganisms and metabolic processes involved are far from being exhaustively characterized. In this work, samples of a domestic sewage treatment plant were analyzed in two complementary studies aiming at the characterization of its phylogenetic diversity and the description of new hydrogenases. The first one, combined the analysis of 16S rRNA and [FeFe]-hydrogenase (hydA) genes with statistical tools to estimate richness and diversity of the prokaryotic community at the phylogenetic and functional levels. Phylogenetic analysis showed that all archaeal sequences were affiliated with yet uncultured Euryarchaeota and that Proteobacteria were the most predominant and diversified phylogenetic group within the bacterial library. The putative hydA sequences were identified as hitherto undetected [Fe-Fe]- hydrogenase gene sequences. Diversity statistical analysis confirmed a great richness and diversity of bacterial and hydA sequences retrieved from the sewage sludge sample. In the second approach, a fosmid metagenomic library was constructed and analyzed employing 454- pyrosequencing technology, resulting in approximately 218 Mb of data. Three different classifiers applied allowed a broad overview of the most abundant taxonomic groups due to a huge number of metagenome reads remained unidentified. However, taxonomic analysis revealed Gammaproteobacteria and Deltaproteobacteria, respectively, as the most abundant classes, whereas species of the genus Methanospirillum were dominant among methanogenic Archaea. The analyzes of the microbial community metabolism by means of COG and Carma databases revealed that the degradation of biomass depends on different phylogenetic groups, for instance, Bacteroidia and Gammaproteobacteria were indicated as involved into the degradation of carbohydrate and proteins. Furthermore, the analysis suggested Clostridia and Methanomicrobiales and Methanosarcinales as the main microorganisms producing hydrogen and methane, respectively. Analysis of the six coding sequences of FeFe-hydrogenases identified into the dataset revealed that they represented novel target gene sequences. However, only four of these coding sequences could be detected into the fosmid library by PCR screening. The combined results obtained in this study allowed us to have an insight of the composition and potential metabolism of the microbes residing in the analyzed domestic sewage treatment plant and suggested such environment as a potential reservoir for new hydrogenase genes to biotechnological exploration Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Valeria Maia de, 1966- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Destéfano, Suzete Aparecida Lanza (committee member), Andreote, Fernando Dini (committee member), Vasconcellos, Suzan Pantaroto de (committee member), Souza, Anete Pereira de (committee member).

Subjects/Keywords: Metagenoma; Hidrogênio; Lodo de esgoto; Análise filogenética; Bioinformática; Metagenome; Hidrogen; Sewage sludge; Phylogenetic analysis; Bioinformatic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tomazetto, Geizecler, 1. (2013). Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2: Diversity analysis and bioprospection of microbial metagenome in a H2-producing biogas fermenter. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tomazetto, Geizecler, 1979-. “Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2: Diversity analysis and bioprospection of microbial metagenome in a H2-producing biogas fermenter.” 2013. Thesis, Universidade Estadual de Campinas. Accessed January 16, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vancouver:

Tomazetto, Geizecler 1. Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2: Diversity analysis and bioprospection of microbial metagenome in a H2-producing biogas fermenter. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2021 Jan 16]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomazetto, Geizecler 1. Diversidade e prospecção de metagenoma microbiano em fermentadores de biogás produzindo H2: Diversity analysis and bioprospection of microbial metagenome in a H2-producing biogas fermenter. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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