+publisher:"Universidade Estadual de Campinas" +contributor:("Amaral, Maria Esmeria Corezola do")

Universidade Estadual de Campinas

1. Falcão, Viviane Tannuri Ferreira Lima, 1962-. Importância do contato intercelular no pâncreas endócrino mediado pelas junções celulares e seu papel na patogênese da diabetes mellitus tipo 2: Cell-cell contact mediated by intercellular junctions within the endocrine pancreas and its role in the pathogenesis of type 2 diabetes mellitus.

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317189

► Abstract: Intercellular junctions (IJs) and their CAMs participate in important cellular processes such as adhesion, growth/death and signaling. In the endocrine pancreas, IJs play a… (more)

▼ Abstract: Intercellular junctions (IJs) and their CAMs participate in important cellular processes such as adhesion, growth/death and signaling. In the endocrine pancreas, IJs play a role in regulating islet cytoarchitecture, insulin biosynthesis and secretion. In this PhD thesis, we investigate the islet histology and cellular distribution and content of CAMs (E-, N-, VE-cadherins, ZO-1, ?- and ?-catenins) in the endocrine pancreas of C57BL/6/JUnib mice fed a high fat (HF) diet for a prolonged time period (8 months). After HF diet exposure, mice became obese and displayed marked metabolic disturbances indicative of type 2 diabetes mellitus, such as marked peripheral insulin resistance and hyperglycemia, and moderate hyperinsulinemia. Isolated pancreatic islets of HF-fed mice showed a significant impairment of glucose-stimulated insulin secretion associated with an increase in insulin (isoforms 1 and 2) gene expression as revealed by qPCR. Histology of the endocrine pancreas revealed no marked changes in islet morphology and cytoarchitecture between animal groups, although HF-fed mice showed a 57% increase in the relative beta cell volume (per total pancreas) in comparison with controls. As shown by immunohistochemistry, ZO-1, E-, N-cadherin and catenins, were expressed at the intercellular contact site of endocrine cells while VE-cadherin was restricted to the islet vascular compartment. A cellular redistribution of N- and E-cadherin and ?-catenin (from the contact region to the cytoplasm in endocrine cells) and an increase in VE-cadherin islet content were seen in diabetic mice as compared to controls. No significant differences in islet immunoreaction for the other CAMs were observed between the animal groups. As revealed by qPCR, an increase in gene expression of VE- and N-cadherins as well as of ZO-1, not accompanied by significant changes in islet protein content, was observed in isolated islets of diabetic mice as compared to the controls. In conclusion, CAMs are expressed by endocrine and endothelial cells of pancreatic islets and, in particular, the islet distribution/content of N-, E- and VE-cadherins as well as ?-catenin are significantly altered in obese and diabetic mice Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Collares-Buzato, Carla Beatriz, 1965- (advisor), Muniz, Maria Tereza Cartaxo (coadvisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Celular e Estrutural (nameofprogram), Vicente, Cristina Pontes (committee member), Sampaio, Helena Cristina de Lima Barbosa (committee member), Amaral, Maria Esmeria Corezola do (committee member), Bosqueiro, José Roberto (committee member).

Subjects/Keywords: Adesão celular; Junções intercelulares; Ilhotas pancreáticas; Caderinas; Cateninas; Cell adhesion; Intercellular junctions; Islets of Langerhans; Cadherins; Catenins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Falcão, Viviane Tannuri Ferreira Lima, 1. (2014). Importância do contato intercelular no pâncreas endócrino mediado pelas junções celulares e seu papel na patogênese da diabetes mellitus tipo 2: Cell-cell contact mediated by intercellular junctions within the endocrine pancreas and its role in the pathogenesis of type 2 diabetes mellitus. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/317189

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Falcão, Viviane Tannuri Ferreira Lima, 1962-. “Importância do contato intercelular no pâncreas endócrino mediado pelas junções celulares e seu papel na patogênese da diabetes mellitus tipo 2: Cell-cell contact mediated by intercellular junctions within the endocrine pancreas and its role in the pathogenesis of type 2 diabetes mellitus.” 2014. Thesis, Universidade Estadual de Campinas. Accessed January 23, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317189.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Falcão, Viviane Tannuri Ferreira Lima, 1962-. “Importância do contato intercelular no pâncreas endócrino mediado pelas junções celulares e seu papel na patogênese da diabetes mellitus tipo 2: Cell-cell contact mediated by intercellular junctions within the endocrine pancreas and its role in the pathogenesis of type 2 diabetes mellitus.” 2014. Web. 23 Jan 2021.

Vancouver:

Falcão, Viviane Tannuri Ferreira Lima 1. Importância do contato intercelular no pâncreas endócrino mediado pelas junções celulares e seu papel na patogênese da diabetes mellitus tipo 2: Cell-cell contact mediated by intercellular junctions within the endocrine pancreas and its role in the pathogenesis of type 2 diabetes mellitus. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Jan 23]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317189.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Falcão, Viviane Tannuri Ferreira Lima 1. Importância do contato intercelular no pâncreas endócrino mediado pelas junções celulares e seu papel na patogênese da diabetes mellitus tipo 2: Cell-cell contact mediated by intercellular junctions within the endocrine pancreas and its role in the pathogenesis of type 2 diabetes mellitus. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/317189

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

2. Costa, Rafaela, 1984-. Ação modulatória da estimulação tátil e do enriquecimento ambiental sobre as respostas hormonais e comportamentais induzidas por estresse crônico, em ratos: The modulatory effects of tactile stimulation and environmental enrichment on hormonal and behavioral responses induced by chronic stress in rats.

Degree: 2014, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314212

► Abstract: Data from the World Health Organization show that depression currently affects 121 million people and will probably be the most common disease in the… (more)

▼ Abstract: Data from the World Health Organization show that depression currently affects 121 million people and will probably be the most common disease in the world in 2020. Scientific evidences show that it is associated with chronic stress, neuronal changes depression-related, anxiety and impairment in learning and memory. There are also a significant association between stress and hyperactivity of the renin angiotensin system on behavioral changes and cardiovascular effects. It is also noteworthy that the quality of the environment in which the individual belongs and the social support represent the main factors that may regulated these effects. In addition, the pathophysiological mechanisms involved in the association between stress and depression, and the protective effects of favorable environmental conditions remain unclear. In animal models, environmental intervention through tactile stimulation (handling) or environmental enrichment have been proposed as factors that may reduce stress and promote well-being. In the experiment 1, the objective of this study was to evaluate the effects of environmental intervention, through tactile stimulation (handling) or environmental enrichment on behavioral and hormonal responses induced by chronic mild unpredictable stress (CMS) in young Sprague-Dawley rats. The results of this study showed that the CMS increased serum corticosterone, plasma norepinephrine and epinephrine concentrations; induced depression-like behaviors; impaired learning and memory and increased hypothalamus angiotensin I, II and IV. Handling and environmental enrichment attenuated stress hormones secretion, depression-like behaviors and increased hypothalamic angiotensin II levels; and cancelled impairment of learning and memory induced by CMS. Considering that the aim of study 1 was evaluate if the effects of angiotensin II, mediated by angiotensin II type 1 receptor (AT1), are involved in hormone responses and cognitive impairment induced by CMS, the experiment 2 were performed. In the second study, male Sprague-Dawley rats controls and stressed, treated or not with losartan (AT1 receptor antagonist) were evaluated. Results from the second study showed that the losartan administration cancelled the increase in stress hormones secretion, attenuated depression-like behaviors and reduced the impairment of learning and memory in stressed animals. The data obtained indicated that the handling or environmental enrichment produced positive hormonal and behavioral effects capable of improving the animal¿s welfare, diminishing the deleterious effects induced by chronic stress in adult rats. The results also suggest that renin-angiotensin system over activity seems to be involved in negative effects of CMS Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Marcondes, Fernanda Klein, 1970- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Funcional e Molecular (nameofprogram), Napimoga, Juliana Trindade Clemente (committee member), Amaral, Maria Esmeria Corezola do (committee member), Spadari, Regina Célia (committee member), Panissi, Christie Ramos Andrade Leite (committee member).

Subjects/Keywords: Estresse; Aprendizagem; Memória; Depressão; Sistema renina-angiotensina; Modelos animais; Stress; Learning; Memory; Depression; Renin-angiotensin system; Models, animal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Costa, Rafaela, 1. (2014). Ação modulatória da estimulação tátil e do enriquecimento ambiental sobre as respostas hormonais e comportamentais induzidas por estresse crônico, em ratos: The modulatory effects of tactile stimulation and environmental enrichment on hormonal and behavioral responses induced by chronic stress in rats. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Costa, Rafaela, 1984-. “Ação modulatória da estimulação tátil e do enriquecimento ambiental sobre as respostas hormonais e comportamentais induzidas por estresse crônico, em ratos: The modulatory effects of tactile stimulation and environmental enrichment on hormonal and behavioral responses induced by chronic stress in rats.” 2014. Thesis, Universidade Estadual de Campinas. Accessed January 23, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Costa, Rafaela, 1984-. “Ação modulatória da estimulação tátil e do enriquecimento ambiental sobre as respostas hormonais e comportamentais induzidas por estresse crônico, em ratos: The modulatory effects of tactile stimulation and environmental enrichment on hormonal and behavioral responses induced by chronic stress in rats.” 2014. Web. 23 Jan 2021.

Vancouver:

Costa, Rafaela 1. Ação modulatória da estimulação tátil e do enriquecimento ambiental sobre as respostas hormonais e comportamentais induzidas por estresse crônico, em ratos: The modulatory effects of tactile stimulation and environmental enrichment on hormonal and behavioral responses induced by chronic stress in rats. [Internet] [Thesis]. Universidade Estadual de Campinas; 2014. [cited 2021 Jan 23]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Costa, Rafaela 1. Ação modulatória da estimulação tátil e do enriquecimento ambiental sobre as respostas hormonais e comportamentais induzidas por estresse crônico, em ratos: The modulatory effects of tactile stimulation and environmental enrichment on hormonal and behavioral responses induced by chronic stress in rats. [Thesis]. Universidade Estadual de Campinas; 2014. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

3. Azevedo, Flávia Figueiredo, 1978-. Insulina tópica modula a fase inflamatória e proliferativa do processo de cicatrização de queimaduras em ratos diabéticos: Topical insulin modulates the inflammatory and proliferative phase of burns wound healing in diabetic rats.

Degree: 2016, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322665

► Abstract: Introduction: Burns are wounds caused by exposure to an electrical source agent, radioactive, chemical or thermal, characterized by delayed inflammatory response. Experimental evidence has… (more)

▼ Abstract: Introduction: Burns are wounds caused by exposure to an electrical source agent, radioactive, chemical or thermal, characterized by delayed inflammatory response. Experimental evidence has shown that the topical insulin accelerates tissue reconstruction incisional wounds in diabetic mice. Though it is not fully clarified the molecular and cellular mechanisms of the healing process and the use of insulin injured by burns. Objective: To analyze the effect of topical insulin on the healing of second-degree burns process using diabetic rats induced with streptozotocin. Methods: Male rats were divided into four groups: controls treated with placebo cream (CP), controls treated with topical insulin (CI), diabetics treated with placebo cream (DP) and diabetics treated with topical insulin (DI). The burn was conducted by means of a mold (1 cm2) heated to 120° C above the skin of the dorsum of each animal during 20 seconds. Burned rats received topical treatment with placebo cream or insulin (PI 0705370-3) once daily. In 7 and 14 days post burn was performed wound area extraction to following analyzes: ELISA, immunohistochemistry, immunoblotting and histological staining with Verhöeff and Weigert, for evaluation of elastic fibers. Results: On 7th day post burn, animals in the DI group had a greater expression of pro-inflammatory cytokines (IL-1ß and IL-6), compared to the DP group (DI vs. DP, p <0.05) and greater ocher immunohistochemistry positive for MCP-1 (monócito- chemoattractant protein 1, p <0.05), F4/80 (macrophage marker activity, p <0.05) and TGF-ß1, p <0.05. On the 14th day after burn, the animals in the DI group observed a higher positive immunostaining ocher of pro-angiogenic factors (TGF-ß1 and VEGF), p <0.05 and 'alpha'-smooth muscle actin ('alpha'-SMA) present on mature blood vessels, p <0.05, compared to the DP group. Topical Insulin also showed increased cell proliferation (anti-Ki67, p <0.05) in the diabetic animals (DI), when compared to the DP group. For Verhöeff and Weigert staining observed that the topical insulin accelerated synthesis and organization of elastic fibers. Conclusion: Treatment with topical insulin accelerated the wound healing in diabetic animals by the recovery of the inflammatory response, angiogenesis, cell proliferation, migration and elastogenesis Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Lima, Maria Helena de Melo, 1966- (advisor), Universidade Estadual de Campinas. Faculdade de Enfermagem (institution), Programa de Pós-Graduação em Enfermagem (nameofprogram), Gamba, Mônica Antar (committee member), Amaral, Maria Esmeria Corezola do (committee member), Thyssen, Patricia Jacqueline (committee member), Araujo, Eliana Pereira de (committee member).

Subjects/Keywords: Insulina; Queimaduras; Inflamação; Tecido elástico; Enfermagem; Insulin; Burns; Inflammation; Elastic tissue; Nursing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Azevedo, Flávia Figueiredo, 1. (2016). Insulina tópica modula a fase inflamatória e proliferativa do processo de cicatrização de queimaduras em ratos diabéticos: Topical insulin modulates the inflammatory and proliferative phase of burns wound healing in diabetic rats. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/322665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Azevedo, Flávia Figueiredo, 1978-. “Insulina tópica modula a fase inflamatória e proliferativa do processo de cicatrização de queimaduras em ratos diabéticos: Topical insulin modulates the inflammatory and proliferative phase of burns wound healing in diabetic rats.” 2016. Thesis, Universidade Estadual de Campinas. Accessed January 23, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/322665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Azevedo, Flávia Figueiredo, 1978-. “Insulina tópica modula a fase inflamatória e proliferativa do processo de cicatrização de queimaduras em ratos diabéticos: Topical insulin modulates the inflammatory and proliferative phase of burns wound healing in diabetic rats.” 2016. Web. 23 Jan 2021.

Vancouver:

Azevedo, Flávia Figueiredo 1. Insulina tópica modula a fase inflamatória e proliferativa do processo de cicatrização de queimaduras em ratos diabéticos: Topical insulin modulates the inflammatory and proliferative phase of burns wound healing in diabetic rats. [Internet] [Thesis]. Universidade Estadual de Campinas; 2016. [cited 2021 Jan 23]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322665.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Azevedo, Flávia Figueiredo 1. Insulina tópica modula a fase inflamatória e proliferativa do processo de cicatrização de queimaduras em ratos diabéticos: Topical insulin modulates the inflammatory and proliferative phase of burns wound healing in diabetic rats. [Thesis]. Universidade Estadual de Campinas; 2016. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/322665

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

4. Portovedo, Mariana, 1987-. A regulação da autofagia mediada por chaperonas no hipotálamo em resposta ao excesso de lipídeos = Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload: Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload.

Degree: 2018, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331810

► Abstract: Obesity has become a global health issue and is directly related to increased risk of developing other metabolic diseases. A healthy lifestyle, including a… (more)

▼ Abstract: Obesity has become a global health issue and is directly related to increased risk of developing other metabolic diseases. A healthy lifestyle, including a balanced diet, is essential for the maintenance of a healthy body mass. Mammals have a powerful system responsible for the regulation of energy homeostasis. Neurons from hypothalamus are responsible for the coupling between food intake and satiety. These neurons are affected by lipid excess, especially long-chain saturated fatty acids and obesity. A subclinical inflammatory state is the hallmark of obesity. Also, obesity leads to the dysregulation of several systems related to stress response. In mice with diet-induced obesity, there is a failure in the regulation of macroautophagy and ubiquitin-proteasome system in hypothalamic neurons. These systems are responsible for the cytosolic protein renewal and therefore for cellular proteostasis. This work aimed to study the chaperone-mediated autophagy modulation in obese mice. This type of autophagy is responsible for the degradation of proteins which have a specific amino acid motif. There are two main proteins responsible for chaperone-mediated autophagy machinery, called HSC-70 and LAMP-A. Our results showed an upregulation in chaperone-mediated autophagy in the hypothalamus of mice fed a high-fat diet for 3 days. Longer exposure to high-fat diet led to the downregulation in hypothalamic chaperone-mediated autophagy. Furthermore, direct exposure of hypothalamic neurons to palmitate, a saturated fatty acid, led to chaperone-mediated autophagy activation. After an insult, the activation of chaperone-mediated autophagy is essential for proteostasis maintenance. On the other hand, failure in this system could lead to increased cellular susceptibility to stressors. Thus, saturated fatty acid excess affects neuronal chaperone-mediated autophagy and this could contribute to neuronal dysfunction and protein aggregate formation in hypothalamic neurons. Therefore, the maintenance of basal chaperone-mediated autophagy levels with pharmacology or dietary interventions could represent an important therapy in the treatment of metabolic disorders Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Milanski, Marciane, 1972- (advisor), Torsoni, Adriana Souza, 1973- (coadvisor), Universidade Estadual de Campinas. Faculdade de Ciências Aplicadas (institution), Programa de Pós-Graduação em Ciências da Nutrição e do Esporte e Metabolismo (nameofprogram), Velloso, Licio Augusto (committee member), Rodrigues, Hosana Gomes (committee member), Moura, Rodrigo Ferreira de (committee member), Amaral, Maria Esmeria Corezola do (committee member).

Subjects/Keywords: Obesidade; Autofagia; Ácido palmítico; Hipotálamo; Obesity; Autophagy; Palmitic acid; Hypothalamus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Portovedo, Mariana, 1. (2018). A regulação da autofagia mediada por chaperonas no hipotálamo em resposta ao excesso de lipídeos = Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload: Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/331810

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Portovedo, Mariana, 1987-. “A regulação da autofagia mediada por chaperonas no hipotálamo em resposta ao excesso de lipídeos = Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload: Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload.” 2018. Thesis, Universidade Estadual de Campinas. Accessed January 23, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/331810.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Portovedo, Mariana, 1987-. “A regulação da autofagia mediada por chaperonas no hipotálamo em resposta ao excesso de lipídeos = Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload: Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload.” 2018. Web. 23 Jan 2021.

Vancouver:

Portovedo, Mariana 1. A regulação da autofagia mediada por chaperonas no hipotálamo em resposta ao excesso de lipídeos = Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload: Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload. [Internet] [Thesis]. Universidade Estadual de Campinas; 2018. [cited 2021 Jan 23]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331810.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Portovedo, Mariana 1. A regulação da autofagia mediada por chaperonas no hipotálamo em resposta ao excesso de lipídeos = Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload: Regulation of hypothalamic chaperone-mediated autophagy in response to lipid overload. [Thesis]. Universidade Estadual de Campinas; 2018. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/331810

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

5. Dorighello, Gabriel Gabriel, 1982-. Efeitos da restrição calórica e do 2,4 dinitrofenol sobre o metabolismo e desenvolvimento da aterosclerose em camundongos hipercolesterolêmicos: Effects of caloric restriction and 2,4-dinitrophenol on the metabolism and development of atherosclerosis in hypercholesterolemic mice.

Degree: 2013, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314294

► Abstract: Cardiovascular diseases (CVD) are the major cause of death in the occidental world. Atherosclerosis is the main process responsible for the pathogenesis of these… (more)

▼ Abstract: Cardiovascular diseases (CVD) are the major cause of death in the occidental world. Atherosclerosis is the main process responsible for the pathogenesis of these diseases. Metabolic disturbances such as dyslipidemias, hypertension, insulin resistance and obesity are well established atherosclerosis risk factors. Currently, different pharmacological and dietetic interventions have been used to reduce and avoid CVD. Caloric restriction is one of these strategies, which is associated with increases in life span and reductions of age related chronic diseases. The mitochondrial uncoupler 2,4 dinitrophenol (DNP) has been considered as a caloric restriction mimetic tool. We have demonstrated that a mouse model of human familial hypercholesterolemia exhibits metabolic and redox disturbances highly relevant for metabolic syndrome and atherosclerosis. Thus, the aim of this study was to evaluate the effects of caloric restriction and mild mitochondrial uncoupling induced by DNP on the metabolism and atherosclerosis development in hypercholesterolemic LDL receptor deficient mice (R0). Thus, R0 mice were compared after 3 months in three conditions: R0 fed ad libitum (control), R0 under caloric restriction (R0-RC) obtained by providing food in alternate days, and R0-DNP fed ad libitum and treated with DNP 1 mg/L in the drinking water. The caloric restriction regimen, despite reducing food intake, induced unexpected adverse metabolic effects, namely, increased plasma total-, VLDL- and LDL-cholesterol, and increased visceral and subcutaneous adipose tissue masses. In addition, R0-RC mice became hyperglycemic, hyperinsulinemic, glucose intolerant and insulin resistant. Moreover, R0-RC mice presented elevated plasma levels of the inflammatory markers TNF? and C-reactive protein. Some benefits regarding systemic and tissue redox state were observed in R0-RC, however, atherosclerosis lesion areas were increased about 3 fold in R0-RC mice. DNP treatment had minimal effects on the lipid and glucose metabolism of R0-DNP mice. It decreased plasma free fatty acids, but did not change other plasma lipids, inflammatory markers, glucose homeostasis, and the size of adipose depots. On the other hand, marked effects of DNP treatment on the tissue redox state were observed, namely, reduction in the generation of mitochondrial oxidative reactive species and in the liver content of carbonyl protein. The spontaneous atherosclerosis development was significantly reduced in the R0-DNP mice, independently other well established risk factors such as plasma cholesterol and inflammatory mediators. Therefore, the caloric restriction regimen is not indicated for the condition of familial hypercholesterolemia due to LDL receptor deficiency. On the other hand, DNP treatment, possibly via mild mitochondrial uncoupling, evidences the relevance of mitochondrial source of reactive oxygen for the atherosclerosis development independently of other risk factors Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Oliveira, Helena Coutinho Franco de, 1958- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Biologia Funcional e Molecular (nameofprogram), Davel, Ana Paula Couto (committee member), Castilho, Roger Frigério (committee member), Amaral, Maria Esmeria Corezola do (committee member), Quintilho, Eder Carlos Rocha (committee member).

Subjects/Keywords: Hipercolesterolemia; Aterosclerose; Restrição calórica; Diabetes Mellitus; Hypercholesterolemia; Atherosclerosis; Caloric restriction; Diabetes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dorighello, Gabriel Gabriel, 1. (2013). Efeitos da restrição calórica e do 2,4 dinitrofenol sobre o metabolismo e desenvolvimento da aterosclerose em camundongos hipercolesterolêmicos: Effects of caloric restriction and 2,4-dinitrophenol on the metabolism and development of atherosclerosis in hypercholesterolemic mice. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/314294

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dorighello, Gabriel Gabriel, 1982-. “Efeitos da restrição calórica e do 2,4 dinitrofenol sobre o metabolismo e desenvolvimento da aterosclerose em camundongos hipercolesterolêmicos: Effects of caloric restriction and 2,4-dinitrophenol on the metabolism and development of atherosclerosis in hypercholesterolemic mice.” 2013. Thesis, Universidade Estadual de Campinas. Accessed January 23, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314294.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dorighello, Gabriel Gabriel, 1982-. “Efeitos da restrição calórica e do 2,4 dinitrofenol sobre o metabolismo e desenvolvimento da aterosclerose em camundongos hipercolesterolêmicos: Effects of caloric restriction and 2,4-dinitrophenol on the metabolism and development of atherosclerosis in hypercholesterolemic mice.” 2013. Web. 23 Jan 2021.

Vancouver:

Dorighello, Gabriel Gabriel 1. Efeitos da restrição calórica e do 2,4 dinitrofenol sobre o metabolismo e desenvolvimento da aterosclerose em camundongos hipercolesterolêmicos: Effects of caloric restriction and 2,4-dinitrophenol on the metabolism and development of atherosclerosis in hypercholesterolemic mice. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2021 Jan 23]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314294.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dorighello, Gabriel Gabriel 1. Efeitos da restrição calórica e do 2,4 dinitrofenol sobre o metabolismo e desenvolvimento da aterosclerose em camundongos hipercolesterolêmicos: Effects of caloric restriction and 2,4-dinitrophenol on the metabolism and development of atherosclerosis in hypercholesterolemic mice. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/314294

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Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations