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You searched for +publisher:"U of Massachusetts : Masters" +contributor:("Paul L Dubin"). One record found.

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1. Flanagan, Sean E. Hetero-Protein Coacervation and Complex Equilibria Between β-lactoglobulin and Lactoferrin.

Degree: MS(M.S.), Chemistry, 2014, U of Massachusetts : Masters

Coacervation between the milk proteins β-lactoglobulin (BLG) and Lactoferrin (LF) was studied as a model system for hetero-protein coacervation (HPC). Equilibria among BLG/LF complexes and the corresponding speciation were found to control coacervation, which can be quantitatively monitored by turbidimetry. Several methods were used to assess complexation as a function of LF : BLG (mol/mol) mixing ratio (r). Proton release, calculated from a shift in pH when LF is added to BLG, was used to identify regions of complexation. Dynamic light scattering (DLS) was used to determine regions of complexation by relating complex size to stoichiometry. Isothermal titration calorimetry (ITC) was used to measure enthalpies of binding upon addition of LF to BLG. These results are used to show that coacervation is related to speciation, with the LF(BLG2)2 complex as the coacervating species. Advisors/Committee Members: Paul L Dubin.

Subjects/Keywords: Coacervation; Hetero-Protein Coacervation; Protein-Protein Interactions; Complex Equilibrium; Lactoferrin; β-lactoglobulin; Analytical Chemistry; Biochemistry; Food Chemistry; Physical Chemistry; Polymer Chemistry

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APA (6th Edition):

Flanagan, S. E. (2014). Hetero-Protein Coacervation and Complex Equilibria Between β-lactoglobulin and Lactoferrin. (Masters Thesis). U of Massachusetts : Masters. Retrieved from http://scholarworks.umass.edu/theses/1179

Chicago Manual of Style (16th Edition):

Flanagan, Sean E. “Hetero-Protein Coacervation and Complex Equilibria Between β-lactoglobulin and Lactoferrin.” 2014. Masters Thesis, U of Massachusetts : Masters. Accessed June 17, 2019. http://scholarworks.umass.edu/theses/1179.

MLA Handbook (7th Edition):

Flanagan, Sean E. “Hetero-Protein Coacervation and Complex Equilibria Between β-lactoglobulin and Lactoferrin.” 2014. Web. 17 Jun 2019.

Vancouver:

Flanagan SE. Hetero-Protein Coacervation and Complex Equilibria Between β-lactoglobulin and Lactoferrin. [Internet] [Masters thesis]. U of Massachusetts : Masters; 2014. [cited 2019 Jun 17]. Available from: http://scholarworks.umass.edu/theses/1179.

Council of Science Editors:

Flanagan SE. Hetero-Protein Coacervation and Complex Equilibria Between β-lactoglobulin and Lactoferrin. [Masters Thesis]. U of Massachusetts : Masters; 2014. Available from: http://scholarworks.umass.edu/theses/1179

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