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You searched for +publisher:"Texas Woman\'s University" +contributor:("LeMieux, Monique"). One record found.

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1. Thomas, Amber N. Effect of tart cherry polyphenols on osteoclast differentiation and activity.

Degree: 2017, Texas Woman's University

Bone is maintained by an intricate balance between bone formation and bone resorption. The presence of inflammation can contribute to an imbalance in bone homeostasis by enhancing differentiation and activity of osteoclasts, the cells that participate in the breakdown of bone. Polyphenols such as flavonoids found in plant-derived foods have been shown to have anti-inflammatory effects in various tissues. Tart cherries are a rich source of such polyphenolic compounds. Using mouse macrophage cells (RAW 264.7), we examined whether tart cherry polyphenols (TCP) could dose-dependently inhibit the proliferation and activity of RANKL-differentiated osteoclasts under inflammatory conditions. Tartrate resistant acid phosphatase (TRAP) activity and staining of TRAP positive multinucleated cells, used as indicators of osteoclast differentiation and activity, decreased in a dose-dependent manner with TCP treatment. Osteoprotegerin (OPG) expression by osteoclasts was decreased in a dose-dependent manner as well. A significant increase in nitrite concentration was observed with the lower doses of TCP of 50 and 100 μg/mL (p<0.05). However, higher doses of TCP (200 and 300 μg/mL) reduced nitrite concentrations below that of the control that received no TCP treatment (p<0.05). Western blot analyses showed that protein expression of COX-2 followed a similar trend, although results were not statistically significant. Conversely, TCP treatments dose-dependently increased iNOS protein expression with statistical significance noted at doses of 200 and 300 μg/mL. Overall, our findings suggest that the polyphenols associated with tart cherries inhibit the negative effects of osteoclasts on bone health. Advisors/Committee Members: Juma, Shanil (advisor), LeMieux, Monique (advisor), Vijayagopal, Parakat (advisor).

Subjects/Keywords: Biology; Aging; Nutrition; Biological sciences; Health and environmental sciences; Bone; Inflammation; Macrophage; Osteoclastogenesis; Osteoporosis; Polyphenols

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APA (6th Edition):

Thomas, A. N. (2017). Effect of tart cherry polyphenols on osteoclast differentiation and activity. (Thesis). Texas Woman's University. Retrieved from http://hdl.handle.net/11274/9402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Thomas, Amber N. “Effect of tart cherry polyphenols on osteoclast differentiation and activity.” 2017. Thesis, Texas Woman's University. Accessed November 29, 2020. http://hdl.handle.net/11274/9402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Thomas, Amber N. “Effect of tart cherry polyphenols on osteoclast differentiation and activity.” 2017. Web. 29 Nov 2020.

Vancouver:

Thomas AN. Effect of tart cherry polyphenols on osteoclast differentiation and activity. [Internet] [Thesis]. Texas Woman's University; 2017. [cited 2020 Nov 29]. Available from: http://hdl.handle.net/11274/9402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thomas AN. Effect of tart cherry polyphenols on osteoclast differentiation and activity. [Thesis]. Texas Woman's University; 2017. Available from: http://hdl.handle.net/11274/9402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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