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You searched for +publisher:"Texas Tech University" +contributor:("Wang, Shu"). Showing records 1 – 3 of 3 total matches.

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Texas Tech University

1. -7116-0923. Nano-liposome mediated delivery of the anti-diabetic protein E4orf1.

Degree: MS, Nutritional Sciences, 2018, Texas Tech University

Objectives: E4orf1 is an adenoviral-derived protein that promotes cellular glucose uptake by up-regulating AKT phosphorylation. Proof of concept studies show that the transgenic or viral-vector mediated expression of the E4orf1 remarkably enhances glycemic control in animal models. However, a system suitable for delivering the E4orf1 protein in humans is needed for extending the findings to clinical setting. As the first step, here we show construction and testing of nano-liposome, a well-known nano-particle, as a carrier to deliver the E4orf1 protein to 3T3-L1 cells in-vitro Method: GST- tagged E4orf1 protein was encapsulated in nano-liposomes which were prepared using Soy phosphatidylcholine and labeled with Rhodamine-PE (Phosphoethanolamine). The size and polydispersity index (PI) of nano-liposomes were measured using a Brookhaven BI-MAS particle size analyzer. To test the delivery efficacy of the nano-liposomes, murine 3T3-L1 cells were treated with E4orf1-containing nano-liposomes (E4 group) or void nano-liposomes (Void group) and expression determined over time by immunofluorescence. Cell lysates from treated cells were used to examine changes in molecular signaling. Results: The diameter of nano-liposomes was 133.5 nm and 90.7 nm and the PI was 0.2 and 0.1 for E4 and void nano-liposomes respectively. The average encapsulation efficiency was 99% as measured by the Enzyme Immuno Assay using anti-E4orf1 antibodies. The E4 group of cells showed maximum GST and Rhodamine expression at 24 h compared to 2, 4 and 12 h. The Void group showed the expression of Rhodamine, but not GST. The expression of E4orf1 in E4 group of cells was confirmed at 24, 48 and 72 h. Western blot analysis in cells treated for 24, 48 and 72 h showed significant increase in p-AKT expression at 72 h in E4 cells compared to the Void group. Conclusions: We report the first successful delivery of E4orf1 encapsulated liposomes and the expected effect of E4orf1 on cell signaling. It provides the proof of concept for extending the use of nano-particle-mediated delivery system to investigate the anti-diabetic potential of E4orf1 in humans. Advisors/Committee Members: Hegde, Vijay (committee member), Wang, Shu (committee member), Dhurandhar, Nikhil (Committee Chair).

Subjects/Keywords: nano-particles; glycemic control

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APA (6th Edition):

-7116-0923. (2018). Nano-liposome mediated delivery of the anti-diabetic protein E4orf1. (Masters Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/74410

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Author name may be incomplete

Chicago Manual of Style (16th Edition):

-7116-0923. “Nano-liposome mediated delivery of the anti-diabetic protein E4orf1.” 2018. Masters Thesis, Texas Tech University. Accessed October 21, 2019. http://hdl.handle.net/2346/74410.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7th Edition):

-7116-0923. “Nano-liposome mediated delivery of the anti-diabetic protein E4orf1.” 2018. Web. 21 Oct 2019.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-7116-0923. Nano-liposome mediated delivery of the anti-diabetic protein E4orf1. [Internet] [Masters thesis]. Texas Tech University; 2018. [cited 2019 Oct 21]. Available from: http://hdl.handle.net/2346/74410.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

-7116-0923. Nano-liposome mediated delivery of the anti-diabetic protein E4orf1. [Masters Thesis]. Texas Tech University; 2018. Available from: http://hdl.handle.net/2346/74410

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete


Texas Tech University

2. Sun, Ming. Anticancer activities of nanoencapsulated Quercetin in breast cancer cells.

Degree: MS, Nutritional Sciences, 2012, Texas Tech University

Background: Breast cancer is the second leading cause of cancer-related death in women. Quercetin, a natural flavonoid abundantly present in grapes, red wine, onion, broccoli and other leafy green vegetables, is known to possess potent anti-proliferative effects against various malignant cells, but the low level of water solubility and bioavailability in the body makes administering it in therapeutic doses unrealistic. Therefore, the development of appropriate flavonoid nanocarriers could be of great importance to enhance its solubility and cellular bioavailability. We have successfully synthesized quercetin encapsulated nanostructured lipid carrier (Q-NLC). Our hypothesis is that Q-NLC can enhance quercetin stability, solubility and cellular bioavailability, decrease the viability of breast cancer cells, and induce their apoptosis. This research project can help to develop a novel preventive and therapeutic modality for breast cancer. Methods: The stability, solubility and cellular bioavailability of quercetin in MCF-7 and MDA-MB-231 breast cancer cells were measured using a high performance liquid chromatography (HPLC) system. Cell viability and apoptosis in MCF-7 and MDA-MB-231 breast cancer cells were measured using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and Annexin-V/PI (propidium iodide) to detect phosphatidylserine exposure on the surface of apoptotic cells, respectively. Results: Nanoencapsulation significantly increased the stability, solubility, and cellular uptake of quercetin. Q-NLC significantly lowered the proliferation of both MCF-7 and MDA-MB-231 breast cancer cells and induced their apoptosis compared to free quercetin. Cell proliferation decreased significantly in a time- (24h and 48h) and dose-dependent (1 μM to 50 μM) manner. Conclusion: Q-NLC is a promising approach for the prevention and treatment of breast cancer. Advisors/Committee Members: Boylan, Mallory (committee member), Zhang, Ruiwen (committee member), Wang, Shu (Committee Chair).

Subjects/Keywords: Nanostructured Lipid Carrier; Quercetin; Nanoparticle; Breast Cancer

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APA (6th Edition):

Sun, M. (2012). Anticancer activities of nanoencapsulated Quercetin in breast cancer cells. (Masters Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/73883

Chicago Manual of Style (16th Edition):

Sun, Ming. “Anticancer activities of nanoencapsulated Quercetin in breast cancer cells.” 2012. Masters Thesis, Texas Tech University. Accessed October 21, 2019. http://hdl.handle.net/2346/73883.

MLA Handbook (7th Edition):

Sun, Ming. “Anticancer activities of nanoencapsulated Quercetin in breast cancer cells.” 2012. Web. 21 Oct 2019.

Vancouver:

Sun M. Anticancer activities of nanoencapsulated Quercetin in breast cancer cells. [Internet] [Masters thesis]. Texas Tech University; 2012. [cited 2019 Oct 21]. Available from: http://hdl.handle.net/2346/73883.

Council of Science Editors:

Sun M. Anticancer activities of nanoencapsulated Quercetin in breast cancer cells. [Masters Thesis]. Texas Tech University; 2012. Available from: http://hdl.handle.net/2346/73883

3. Chang, Hui. Acute effect of dietary fatty acid composition on postprandial thermogenesis and substrate oxidation in normal weight and obese females.

Degree: PhD, Nutritional Sciences, 2014, Texas Tech University

Background: The Composition of fatty acids in a diet may differentially affect metabolism, thus playing a role in the development of obesity. Our purpose was to study the effects of three high-fat (HF) meals with different dietary fatty acid compositions on diet induced thermogenesis (DIT) and substrate oxidation in obese premenopausal women. Methods: 15 healthy normal weight and 16 obese women, aged 18-39 years, participated in a single-blinded randomized cross over study, in which they consumed isocaloric HF meals (70% of energy from fat) rich in either saturated fat (SFA) (40% of total energy) , monounsaturated fat (MUFA) (42% of total energy) or polyunsaturated fat (PUFA) (42% of total energy). Indirect calorimetry was used to measure respiratory gases for a 5-hour postprandial period. Data collected was used to determine respiratory exchange ratio (RER) for assessing substrate oxidation, and energy expenditure for the determination of DIT. Results: For normal weight women, the area under the curve for DIT following the PUFA-rich HF meal was greater than that of the SFA- or MUFA-rich HF meals (19.9±1.4, 17.1±1.7 and 17.9±2.3 kcals/5-hours (p=0.02) for PUFA, MUFA and SFA, respectively). No significant difference was found in RER (0.86±0.01, 0.85±0.01 and 0.85±0.01 for PUFA, MUFA, and SFA-rich HF meals, respectively) or substrate utilization following the three different HF meals (24.4±1.9, 22.4±1.1 and 23.3±1.9 grams for cumulative postprandial carbohydrate oxidation following the PUFA, MUFA, and SFA-rich HF meals, respectively; 7.7±0.7, 8.3±0.5 and 8.2±0.6 grams for cumulative fat oxidation the PUFA, MUFA, and SFA-rich HF meals, respectively). For obese subjects there was a significant time effect on both substrate oxidation and DIT (p<0.05). No treatment difference was found in DIT (21.6±1.6, 22.0±1.9 and 21.3±1.8 kcals/5 hours for SFA, MUFA, and PUFA-rich HF meals, respectively) or substrate utilization following the three different HF meals (26.4±1.8, 26.5±1.0 and 27.7±1.2 grams for cumulative postprandial carbohydrate oxidation following the SFA, MUFA, and PUFA-rich HF meals, respectively; 9.9±0.8, 9.7±0.6 and 9.2±0.7 grams for cumulative fat oxidation the following SFA, MUFA, and PUFA-rich HF meals, respectively). Conclusions: Acute ingestion of a PUFA-rich HF meal induced a greater DIT in normal weight women compared to SFA- or MUFA-rich HF meals. No significant differences were found for substrate utilization. In obese women, acute ingestion of HF meals enriched in SFA, MUFA or PUFA did not elicit differences in substrate oxidation or DIT. Advisors/Committee Members: Jai, Catherine (committee member), Paton, Chad M. (committee member), Ballou, Michael A. (committee member), Wang, Shu (committee member), Cooper, Jamie A. (Committee Chair).

Subjects/Keywords: Dietary fatty acids; Diet induced thermogenisis; Substrate oxidation; Acute feeding; Respiratory exchange ratio (RER)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Chang, H. (2014). Acute effect of dietary fatty acid composition on postprandial thermogenesis and substrate oxidation in normal weight and obese females. (Doctoral Dissertation). Texas Tech University. Retrieved from http://hdl.handle.net/2346/58713

Chicago Manual of Style (16th Edition):

Chang, Hui. “Acute effect of dietary fatty acid composition on postprandial thermogenesis and substrate oxidation in normal weight and obese females.” 2014. Doctoral Dissertation, Texas Tech University. Accessed October 21, 2019. http://hdl.handle.net/2346/58713.

MLA Handbook (7th Edition):

Chang, Hui. “Acute effect of dietary fatty acid composition on postprandial thermogenesis and substrate oxidation in normal weight and obese females.” 2014. Web. 21 Oct 2019.

Vancouver:

Chang H. Acute effect of dietary fatty acid composition on postprandial thermogenesis and substrate oxidation in normal weight and obese females. [Internet] [Doctoral dissertation]. Texas Tech University; 2014. [cited 2019 Oct 21]. Available from: http://hdl.handle.net/2346/58713.

Council of Science Editors:

Chang H. Acute effect of dietary fatty acid composition on postprandial thermogenesis and substrate oxidation in normal weight and obese females. [Doctoral Dissertation]. Texas Tech University; 2014. Available from: http://hdl.handle.net/2346/58713

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