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Texas A&M University
1.
Corkill, Carolina.
Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells.
Degree: MS, Food Science and Technology, 2013, Texas A&M University
URL: http://hdl.handle.net/1969.1/151340
► Salmonella Typhimurium continues to be a leading cause of human gastroenteritis worldwide. This organism is a facultative intracellular pathogen, meaning that it is able grow…
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▼ Salmonella Typhimurium continues to be a leading cause of human gastroenteritis worldwide. This organism is a facultative intracellular pathogen, meaning that it is able grow and reproduce within the host cell it inhabits. S. Typhimurium has the ability to invade and replicate within human intestinal epithelial cells, which in turn causes induced cell death or apoptosis.
The human intestinal epithelial cells, HCT-8, were challenged with live, heat inactivated, and electron beam inactivated S. Typhimurium for various time points. Infected cell monolayers were collected for RNA extractions, and Real-time PCR was performed on the samples to analyze differential gene expression. Genes of the host cell that were expected to be differentially expressed were shortlisted and Real-Time PCR analysis was performed.
Internalized Salmonella within the host cell was unable to be successfully visualized using fluorescent light microscopy. However, differential gene expression for a common transcriptional regulator and inflammatory chemokine were observed to be expressed significantly higher in response to e-beam inactivated Salmonella infection. Genes coding for extracellular and intracellular pattern-recognition receptors of the host cells were shown to be up-regulated in response to e-beam inactivated Salmonella infection at 4 and 24 hours, but were not statistically significant. Additional studies must be conducted to definitively confirm e-beam irradiated Salmonella has the ability to invade human host cells.
Advisors/Committee Members: Pillai, Suresh D. (advisor), Zhou, Huaijun (advisor), Byrd, James A. (committee member).
Subjects/Keywords: Salmonella; Electron beam irradiation
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APA (6th Edition):
Corkill, C. (2013). Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151340
Chicago Manual of Style (16th Edition):
Corkill, Carolina. “Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells.” 2013. Masters Thesis, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/151340.
MLA Handbook (7th Edition):
Corkill, Carolina. “Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells.” 2013. Web. 27 Feb 2021.
Vancouver:
Corkill C. Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/151340.
Council of Science Editors:
Corkill C. Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151340

Texas A&M University
2.
Trojacek, Erica.
Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course.
Degree: MS, Nutrition, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10289
► In birds, estrogen is a strong stimulator of gene programs that regulate the formation of very low density lipoproteins (VLDL). Apolipoprotein-B (ApoB) is an integral…
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▼ In birds, estrogen is a strong stimulator of gene programs that regulate the formation of very low density lipoproteins (VLDL). Apolipoprotein-B (ApoB) is an integral part of very low density lipoproteins. In mammals, the rate of ApoB synthesis is controlled by post-translational means. In contrast, estrogen treated birds show changes in ApoB transcript level; in a natural setting, the bird?s metabolism and transcription are in great flux due to yolk formation. Besides the ApoB gene, the entire complement of genes that is necessary to form a VLDL is not known. To determine the genes that play a role in the formation of VLDL 7-10d old chicks were injected with estrogen at several time points over a 24hr period. Following exsanguinations by cardiac puncture, livers were removed and RNA was extracted. The RNA was quantified and hybridized to microarrays using a dual-dye system. Slides were scanned and analyzed, and features were extracted. To qualify microarray results, quantitative real time PCR (q-RTPCR) was done on a selection of genes.
Previous studies had shown that approximately 200 genes are upregulated by the treatment of hormone naive chickens with estrogen. As a result of our liver transcriptional profiling, we identified 1,528 genes at 1.5hrs, 1,931 genes at 3hrs, 2,398 genes at 6hrs, 2,356 at 12hrs, and 1,713 genes at 24hrs following estrogen exposure. We determined that these regulated genes include those responsible for the transcription of RNA used to create the gene products that serve as components of VLDL itself or that act in VLDL assembly. These include genes encoding structural proteins, like ApoB, and genes encoding assembly-related proteins. Of the differentially expressed genes as compared to time 0, there were approximately 30% which were unannotated with regards to function limiting conclusions. We hope to determine the function of these genes and to annotate them based on this information.
Advisors/Committee Members: Walzem, Rosemary L. (advisor), Villalobos, Alice (committee member), Zhou, Huaijun (committee member).
Subjects/Keywords: chicken; estrogen; microarray
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APA (6th Edition):
Trojacek, E. (2012). Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10289
Chicago Manual of Style (16th Edition):
Trojacek, Erica. “Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course.” 2012. Masters Thesis, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10289.
MLA Handbook (7th Edition):
Trojacek, Erica. “Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course.” 2012. Web. 27 Feb 2021.
Vancouver:
Trojacek E. Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10289.
Council of Science Editors:
Trojacek E. Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10289

Texas A&M University
3.
Kachroo, Priyanka.
Functional Analyses of the Molecular mechanisms Underlying Two Equine Respiratory Diseases: Recurrent Airway Obstruction and Rhodococcus equi Pneumonia.
Degree: PhD, Biomedical Sciences, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10994
► Recurrent airway obstruction (RAO) and Rhodococcus equi (R. equi) pneumonia are two equine respiratory diseases. RAO is an allergic asthma like disease of the middle-aged…
(more)
▼ Recurrent airway obstruction (RAO) and Rhodococcus equi (R. equi) pneumonia are two equine respiratory diseases. RAO is an allergic asthma like disease of the middle-aged horses while the R. equi pneumonia affects only young foals. Respiratory disease is considered among the major causes of economic loss to the equine industry and tops the priority list for research that will focus on preventative and diagnostic facets of such disease. The objective of this research was to investigate the effect of antigen exposure and remission (via allergen avoidance and/or drug) on chronically affected RAO horses. Additionally, we also wanted to understand the changes in equine neonatal immune system due to R. equi exposure and identify molecular biomarkers for early disease screening. Various biological samples (lung tissue for the RAO study and blood leukocytes and nasal epithelial cells for the R. equi study) were used to extract ribonucleic acid (RNA). Complimentary deoxyribonucleic acid (cDNA) obtained from RNA was used to perform microarray hybridization experiments.
Our findings suggest that compared to control horses allergen exposure leads to an elevated protein synthesis and inflammation that contributes to aggravation of symptoms and airway changes. We found that allergen avoidance controls inflammation and causes an improvement in lung function and other chronic features of RAO. The drug administration led to an accelerated remission in the chronic RAO features; a complete remission could however not be achieved. Hence it appears that although not a complete resolution, but allergen avoidance and drugs will help in a better management of chronic RAO symptoms.
Our results suggest that the neonatal immune system is capable of initiating a protective immune response through birth up to 8 weeks of age. However there are also processes present that may be counter-productive to the host. Induction of such suppressive mechanisms may be a result of bacterial modulation of the host immune response or a result of immature host immune system. We also identified molecular biomarkers that will have the potential to screen foals for R. equi pneumonia soon after birth and before the onset of clinical symptoms. The research findings of this study will improve the current understanding of the two equine diseases.
Advisors/Committee Members: Chowdhary, Bhanu (advisor), Seabury, Christopher (committee member), Ivanov, Ivan (committee member), Zhou, Huaijun (committee member).
Subjects/Keywords: Equine; Microarray; Heaves; Pneumonia
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Kachroo, P. (2012). Functional Analyses of the Molecular mechanisms Underlying Two Equine Respiratory Diseases: Recurrent Airway Obstruction and Rhodococcus equi Pneumonia. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10994
Chicago Manual of Style (16th Edition):
Kachroo, Priyanka. “Functional Analyses of the Molecular mechanisms Underlying Two Equine Respiratory Diseases: Recurrent Airway Obstruction and Rhodococcus equi Pneumonia.” 2012. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10994.
MLA Handbook (7th Edition):
Kachroo, Priyanka. “Functional Analyses of the Molecular mechanisms Underlying Two Equine Respiratory Diseases: Recurrent Airway Obstruction and Rhodococcus equi Pneumonia.” 2012. Web. 27 Feb 2021.
Vancouver:
Kachroo P. Functional Analyses of the Molecular mechanisms Underlying Two Equine Respiratory Diseases: Recurrent Airway Obstruction and Rhodococcus equi Pneumonia. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10994.
Council of Science Editors:
Kachroo P. Functional Analyses of the Molecular mechanisms Underlying Two Equine Respiratory Diseases: Recurrent Airway Obstruction and Rhodococcus equi Pneumonia. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10994

Texas A&M University
4.
Stringfellow, Kendre.
Immunomodulatory Effects of Probiotic and Anticoccidial Treatments in Broiler Chickens.
Degree: PhD, Poultry Science, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11863
► Four experiments evaluated the impact of probiotic administration on the immune response of broilers vaccinated with a live coccidiosis vaccine. Experiment one showed that probiotic…
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▼ Four experiments evaluated the impact of probiotic administration on the immune response of broilers vaccinated with a live coccidiosis vaccine. Experiment one showed that probiotic administration increased heterophil and monocyte oxidative burst, and lymphocyte proliferation at multiple time points. In experiment two, probiotic + vaccine increased heterophil and monocyte oxidative burst on d 15 when compared with the negative controls. Overall, vaccine administration alone showed the highest response when compared to all other treatments. In the second trial, all birds were exposed to Eimeria oocysts in the litter and oral gavaged. The results showed that probiotic + vaccine resulted in greater heterophil and monocyte oxidative burst levels on d 14 and 28 when compared to the negative controls. Increases in lymphocyte proliferation were also seen in the probiotic + vaccine and probiotic alone broilers on d 14 among other treatments.
In experiment three, heterophil oxidative burst was increased (p <= 0.05) in the vaccine alone group, vaccine with probiotic group, and the ionophore with probiotic group, when compared to the negative control. Monocyte oxidative burst was increased (p <= 0.05) in the vaccine with probiotic group on d 36 and 43, compared to the negative control. Lymphocyte proliferation was greater (p <= 0.05) on d 22 and 36 in the ionophore with probiotic group, when compared to the negative control.
Experiment four showed that liver AVBD 2 gene expression elevated (p <= 0.05) in the probiotic + vaccine group relative to the probiotic alone group. Ileum AVBD 2 gene expression was not affected among any of the treatments was evaluated. Liver AVBD 9 was demonstrated to have higher (p <= 0.05) gene expression in the vaccine group when compared to controls. When AVBD 9 gene expression was evaluated in the ileum, a decrease (p <= 0.05) was observed in all treatments compared to the control group. These data suggest that simultaneous administration of probiotics during coccidiosis vaccination or ionophore treatment has the ability to modulate the immune response at varying time points.
Advisors/Committee Members: Farnell, Morgan (advisor), Caldwell, David (committee member), Lee, Jason (committee member), Zhou, Huaijun (committee member).
Subjects/Keywords: Coccidiosis; Probiotic; Immunity; Defensin; Vaccine
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Stringfellow, K. (2012). Immunomodulatory Effects of Probiotic and Anticoccidial Treatments in Broiler Chickens. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11863
Chicago Manual of Style (16th Edition):
Stringfellow, Kendre. “Immunomodulatory Effects of Probiotic and Anticoccidial Treatments in Broiler Chickens.” 2012. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11863.
MLA Handbook (7th Edition):
Stringfellow, Kendre. “Immunomodulatory Effects of Probiotic and Anticoccidial Treatments in Broiler Chickens.” 2012. Web. 27 Feb 2021.
Vancouver:
Stringfellow K. Immunomodulatory Effects of Probiotic and Anticoccidial Treatments in Broiler Chickens. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11863.
Council of Science Editors:
Stringfellow K. Immunomodulatory Effects of Probiotic and Anticoccidial Treatments in Broiler Chickens. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11863

Texas A&M University
5.
Bautista Ortega, Jaime.
Dietary L-Arginine and Antioxidant Vitamins E and C Influence on Cardiovascular Performance in Chickens.
Degree: PhD, Poultry Science, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11138
► Pulmonary hypertension syndrome (PHS) in broiler chickens adequately represents idiopathic pulmonary arterial hypertension (IPAH) in humans, a condition that affects 300 new patients each year…
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▼ Pulmonary hypertension syndrome (PHS) in broiler chickens adequately represents idiopathic pulmonary arterial hypertension (IPAH) in humans, a condition that affects 300 new patients each year in the US. The factors that trigger IPAH are poorly understood but an increase in reactive oxygen species in the circulation coincides with the onset of these conditions. Broiler chickens (n=583) were fed a control diet (CTL), containing 3,200 kcal of ME / kg of feed, 23% CP, 1.55% (wt / wt) Arginine (Arg) and 40 IU of VE (alpha-tochopherol) / kg of feed; a high-Arg diet (HA), CTL diet plus 0.8% (wt / wt) supplemental L-Arg HCl; or a high Arg and vitamin diet (AEC), the HA diet plus 200 IU ?-tochopherol / kg of feed and 500 mg of ascorbic acid / L of drinking water 500 mg ascorbic acid / L of water (exp. 1 and 2) or Kg feed (exp. 3). Supplemented broilers were either exposed to hypobaric hypoxia or had a primary bronchus occluded (PBO) to induce PHS. Also, medial thickness was assessed in male broiler and Leghorn (n =80) chickens fed a CTL diet and subjected to pulmonary artery occlusion (PAO).
The results show that supplementation with Arg and VE plus VC have an additive effect on the velocity at which the pulmonary arterial pressure returned to basal levels in hypoxic chickens challenged with epinephrine. Also, supplementation increased xanthine oxidase (XO) activity in the vicinity of the pulmonary endothelium with no effect on NAD(P)H-oxidase activity or oxidative stress in hypoxic chickens subjected to PBO. These enzymes are upregulated in humans with IPAH. Furthermore, supplementation reduced pulmonary artery reactivity to phenylephrine in hypoxemic broilers. Unsupplemented broiler chickens had a lower specific lung weight compared to unsupplemented Leghorns. Hypoxemic broilers showed thicker resistant pulmonary arteries and were more hypertensive than hypoxemic Leghorns. Leghorns were more hypoxemic and resistant to PHS than broilers. In conclusion, Arg and VE plus VC show an additive effect in the improvement of cardiovascular performance of hypoxemic broilers as well as in restoring reactivity to phenylephrine in hypoxemic pulmonary rings. Also, supplementation shows an additive effect in restoring XO activity in hypoxic broilers. Leghorns had a better ventilation capacity and better pulmonary vasodilation capacity than broiler chickens.
Advisors/Committee Members: Ruiz-Feria, Ciro A. (advisor), Zhou, Huaijun (committee member), Berghman, Luc R. (committee member), Stallone, John N. (committee member).
Subjects/Keywords: Pulmonary hypertension syndrome; Antioxidant vitamins; Arginine; Unilateral pulmonary artery occlusion; Primary bronchus occlusion; Hypobaric hypoxia
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bautista Ortega, J. (2012). Dietary L-Arginine and Antioxidant Vitamins E and C Influence on Cardiovascular Performance in Chickens. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11138
Chicago Manual of Style (16th Edition):
Bautista Ortega, Jaime. “Dietary L-Arginine and Antioxidant Vitamins E and C Influence on Cardiovascular Performance in Chickens.” 2012. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11138.
MLA Handbook (7th Edition):
Bautista Ortega, Jaime. “Dietary L-Arginine and Antioxidant Vitamins E and C Influence on Cardiovascular Performance in Chickens.” 2012. Web. 27 Feb 2021.
Vancouver:
Bautista Ortega J. Dietary L-Arginine and Antioxidant Vitamins E and C Influence on Cardiovascular Performance in Chickens. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11138.
Council of Science Editors:
Bautista Ortega J. Dietary L-Arginine and Antioxidant Vitamins E and C Influence on Cardiovascular Performance in Chickens. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11138
6.
Halim, Vera.
Involvement of PFKFB3/iPFK2 in the Effects of Leucine and n-3 PUFA in Adipocytes.
Degree: MS, Nutrition, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10668
► Studies had shown that leucine supplementation increases insulin sensitivity and it has been studied that n-3 PUFA may have an anti-inflammatory effect in adipocytes. However,…
(more)
▼ Studies had shown that leucine supplementation increases insulin sensitivity and it has been studied that n-3 PUFA may have an anti-inflammatory effect in adipocytes. However, the extent to which dietary sources such as leucine and/or n-3 PUFA act through PFKFB3/iPFK2 to suppress adipocyte inflammatory response has not been studied; PFKFB3/iPFK2 is a regulator that links adipocyte metabolism and inflammatory responses. In this study, the involvement of PFKFB3/iPFK2 in the effects of insulin sensitizing and anti-inflammatory effect of leucine and/or n-3 PUFA are explored using cultured 3T3-L1 adipocytes including wild-type cells, PFKFB3-control cells (iPFK2-Ctrl) and PFKFB3-knockdown cells (iPFK2-KD).
In iPFK2-Ctrl cells, leucine supplementation appears to have insulin-sensitizing effects through improving p-Akt/Akt insulin signaling, but have no effect on adiponectin expression, and appear to have limited anti-inflammatory effects. n-3 PUFA supplementation appears to have limited effects on both insulin sensitizing and anti-inflammatory effects in iPFK2-Ctrl. In contrast, n-3 PUFA exhibit pro-inflammatory expression in iPFK2-KD. The results of this study support the hypothesis that PFKFB3/iPFK2 is critically involved in insulin-sensitizing effects of leucine. This role of PFKFB3/iPFK2, however, appears to be independent of anti-inflammatory responses. Given this, it is likely that PFKFB3/iPFK2 only account, in part, for the beneficial effects of leucine. n-3 PUFA stimulate PFKFB3/iPFK2 activity in wild-type adipocytes. However, PUFA do not exhibit anti-inflammatory and insulin-sensitizing effects in controls. In contrast, n3-PUFA exhibit proinflammatory effects in iPFK2-KD cells. Taken together, PFKFB3/iPFK2 is involved, at least in part, in the effects of insulin sensitization of leucine and appears to protect adipocytes from inflammatory responses, which could be exacerbated by n-3 PUFA when PFKFB3/iPFK2 is disrupted.
Advisors/Committee Members: Wu, Chaodong (advisor), Chapkin, Robert (committee member), Zhou, Huaijun (committee member).
Subjects/Keywords: PFKFB3/iPFK2; leucine; n-3 PUFA; adipocytes; insulin; inflammation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Halim, V. (2012). Involvement of PFKFB3/iPFK2 in the Effects of Leucine and n-3 PUFA in Adipocytes. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10668
Chicago Manual of Style (16th Edition):
Halim, Vera. “Involvement of PFKFB3/iPFK2 in the Effects of Leucine and n-3 PUFA in Adipocytes.” 2012. Masters Thesis, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10668.
MLA Handbook (7th Edition):
Halim, Vera. “Involvement of PFKFB3/iPFK2 in the Effects of Leucine and n-3 PUFA in Adipocytes.” 2012. Web. 27 Feb 2021.
Vancouver:
Halim V. Involvement of PFKFB3/iPFK2 in the Effects of Leucine and n-3 PUFA in Adipocytes. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10668.
Council of Science Editors:
Halim V. Involvement of PFKFB3/iPFK2 in the Effects of Leucine and n-3 PUFA in Adipocytes. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10668
7.
Wang, Ying.
Genomics Approaches to Study Molecular and Cellular Mechanisms of Host Response to Avian Influenza Virus in Chickens.
Degree: PhD, Poultry Science, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10701
► Avian influenza virus (AIV) is a type A virus of the family Orthomyxoviridae and its outbreaks not only cause economic losses in poultry, but also…
(more)
▼ Avian influenza virus (AIV) is a type A virus of the family Orthomyxoviridae and its outbreaks not only cause economic losses in poultry, but also are worldwide threats to human health. The phenotypic changes in host cells induced by pathogens are always accompanied by remarkable changes in gene expression. Therefore understanding the gene expression profile of infected cells at the global level is important to get insights into interactions between hosts and viruses. Different genomic approaches have been utilized in the current study to investigate the host-AIV interactions in chickens. The Ser to Asn mutation on position 631 in the chicken Mx1 protein was reported to result in a positive antiviral function in vitro. With AIV infection, the Mx1 mRNA expression levels in heterozygous birds were significantly up-regulated. Additional mutations on the chicken Mx1 coding region were identified by sequencing. The results showed that most identified mutations were co-segregated with S631N mutation except one insertion in the position of 1544bp in the heterozygous birds. We speculate this insertion might be related to the up-regulation of mRNA expression of heterozygous birds with AIV infection. The miRNAs play critical roles in biological processes and are important effectors in host-pathogen interactions. The miRNA deep sequencing was used to profile miRNAs in AIV infected or non-infected chickens. Differentially expressed miRNAs identified have expanded our knowledge in the functions of these potential immune related chicken miRNAs regulating host response to AIV infection. Both microarray and transcriptome analysis by RNA-Seq were used in the current study to investigate the global gene expression of host response to AIV infection. Through the comprehensive analysis, a list of strong candidate miRNAs such as miR-32 and their host target genes including Mx1 were identified for further elucidating the regulatory mechanism of host-AIV interaction. In summary, we have identified many important candidate host genes and miRNAs which play important roles in the modulation of host response to AIV infection using genomic approaches. Further investigation of underline regulatory mechanisms of these genes, miRNAs or related pathways, followed by functional analysis, could lay solid foundation for understanding cellular and molecular mechanisms of the host-AIV interactions, thereby, pave a way for the development of novel protective strategies against AIV infection in chickens.
Advisors/Committee Members: Zhou, Huaijun (advisor), Lupiani, Blanca (committee member), Pillai, Suresh D. (committee member), Berghman, Luc R. (committee member), Bailey, Michael J. (committee member).
Subjects/Keywords: Chicken; Avian influenza (AI); Virus; Genomic approaches
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, Y. (2012). Genomics Approaches to Study Molecular and Cellular Mechanisms of Host Response to Avian Influenza Virus in Chickens. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10701
Chicago Manual of Style (16th Edition):
Wang, Ying. “Genomics Approaches to Study Molecular and Cellular Mechanisms of Host Response to Avian Influenza Virus in Chickens.” 2012. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10701.
MLA Handbook (7th Edition):
Wang, Ying. “Genomics Approaches to Study Molecular and Cellular Mechanisms of Host Response to Avian Influenza Virus in Chickens.” 2012. Web. 27 Feb 2021.
Vancouver:
Wang Y. Genomics Approaches to Study Molecular and Cellular Mechanisms of Host Response to Avian Influenza Virus in Chickens. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10701.
Council of Science Editors:
Wang Y. Genomics Approaches to Study Molecular and Cellular Mechanisms of Host Response to Avian Influenza Virus in Chickens. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10701
8.
Hinojosa-Garza, Carolee A.
Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System.
Degree: MS, Poultry Science, 2013, Texas A&M University
URL: http://hdl.handle.net/1969.1/151042
► Poultry transport coops are rarely washed and demonstrate to be a major point of broiler carcass contamination. Our laboratory hypothesized that foaming disinfectants and cleaners…
(more)
▼ Poultry transport coops are rarely washed and demonstrate to be a major point of broiler carcass contamination. Our laboratory hypothesized that foaming disinfectants and cleaners commonly used within processing plants may be used to clean and disinfect poultry transport coops. The objective of this study was to evaluate treatments consisting of a low-pressure water rinse (LPWR), a foaming additive alone, foaming cleaner or peroxyacetic acid with a foaming additive to reduce bacteria on broiler transport coops. A high-pressure water rinse (HPWR) applied prior to and following treatments was also evaluated. Homogenized feces was evenly applied to the floors of pre-cleaned transport coops and allowed to dry. The first study used fresh layer feces and evaluated the treatments ability to reduce aerobic bacteria from the manure. The second study added a HPWR step to determine whether this technique would reduce bacteria. In the third study, Salmonella Typhimurium was added to the homogenized fecal slurry to evaluate how effectively these methods reduce aerobic bacteria and Salmonella on coop surfaces. The field study utilized laboratory treatments proven to be most effective on freshly soiled broiler integrator coops.
All foaming treatments were applied using a compressed air foam system (CAFS) using a 1 inch fire hose. Ten minutes post-treatment, all surfaces were rinsed with a LPWR for 30 seconds to remove residual disinfectant. Samples were collected from the transport coops prior to and following treatments utilizing a flame sterilized 5 x 5 cm stainless steel template and a gauze swab pre-applied with buffered peptone water. All samples were stomached, serially diluted, spread plated onto agar plates, incubated for 24 h at 37° C and enumerated. The foam cleaner and peroxyacetic acid with a foam additive significantly reduced (P < 0.05) aerobic bacteria up to 4.84 to 5.17 logs, respectively when compared to the LPWR. The addition of a HPWR following product application significantly reduced bacteria on integrator coops, in the field study, but didn’t improve efficacy of our treatments in laboratory trials. These data suggest that a CAFS may be used in combination with disinfectants and cleaners to reduce bacteria on poultry transport coops.
Advisors/Committee Members: Caldwell, David J (advisor), Farnell, Morgan B (advisor), Byrd, James A (committee member), Zhou, Huaijun (committee member), Kogut, Michael H (committee member).
Subjects/Keywords: Cleaning and disinfection; biosecurity; food safety; transportation coops; poultry
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APA (6th Edition):
Hinojosa-Garza, C. A. (2013). Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151042
Chicago Manual of Style (16th Edition):
Hinojosa-Garza, Carolee A. “Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System.” 2013. Masters Thesis, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/151042.
MLA Handbook (7th Edition):
Hinojosa-Garza, Carolee A. “Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System.” 2013. Web. 27 Feb 2021.
Vancouver:
Hinojosa-Garza CA. Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/151042.
Council of Science Editors:
Hinojosa-Garza CA. Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151042

Texas A&M University
9.
Chiang, Hsin-I.
Genomic Approaches to Study Innate Immune Response to Salmonella Enteritidis Infection in Chickens.
Degree: PhD, Poultry Science, 2010, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-12-244
► Salmonella enterica serovar Enteritidis (SE) is one of the most common food-borne pathogens that cause human salmonellosis. Contamination of consumed poultry products continues to be…
(more)
▼ Salmonella enterica serovar Enteritidis (SE) is one of the most common food-borne
pathogens that cause human salmonellosis. Contamination of consumed poultry products
continues to be a global threat to public health. Genetic resistance using genomic
approach provides a promising solution to controlling SE infection in poultry. The
mechanism of SE resistance in chickens remains elusive. Three different approaches,
microarray techology, gene silencing, and computational gene analysis, have been
utilized to study SE-induced transcriptional changes of host immune response in the
chicken.
A whole genome chicken 44K microarray was used to analyze the transcriptome of
heterophils from SE-resistant (line A) and SE-susceptible chickens (line B) with/without
in vitro SE stimulation. Many differentially expressed immune-related genes were found
in the SE-infected to non-infected comparison, where more immune-related genes were
down-regulated in line B than line A. These results suggested a similar Toll-like receptor
(TLR) regulatory network might exist in heterophils of both lines, and provided strong
candidates for further investigating SE resistance and susceptibility in chickens. In the gene silencing study, small interfering RNAs (siRNA) were used to specifically inhibit the expression of NFkB1 in the chicken HD11 macrophage cell line with SE challenge.
Genes related to the NF-kB signaling pathway were selected to examine the effect of
NFkB1 inhibition on TLR pathway. With 36% inhibition of NFkB1 expression, the
results showed an increased expression of TLR4 and interleukin (IL)-6 following SE
challenge and suggested a likely inhibitory regulation of NFkB1 on TLR signal pathway.
Finally, two novel chicken C-type lectin-like receptors were identified and annotated to
chicken CD69 and CD94/NKG2-like with multiple evidences generated by computational (in-silico) sequence analysis. Both genes located in a region on chicken
chromosome 1 that is syntenic to mammalian Nature Killer Receptor Complex (NKC)
region, which may have existed before the divergence between mammals and aves.
While siRNA lays the foundation of using loss-of-function approach on testifying
gene-gene interactions, in-silico analysis aids in gathering information of unknown
genes of great interest. Both approaches provide great potential to use for down-stream
analysis following microarray study.
Advisors/Committee Members: Zhou, Huaijun (advisor), Pillai, Suresh D. (committee member), Berghman, Luc R. (committee member), Reddy, Sanjay (committee member).
Subjects/Keywords: chicken; microarray; RNA interference; immune response
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chiang, H. (2010). Genomic Approaches to Study Innate Immune Response to Salmonella Enteritidis Infection in Chickens. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-12-244
Chicago Manual of Style (16th Edition):
Chiang, Hsin-I. “Genomic Approaches to Study Innate Immune Response to Salmonella Enteritidis Infection in Chickens.” 2010. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2008-12-244.
MLA Handbook (7th Edition):
Chiang, Hsin-I. “Genomic Approaches to Study Innate Immune Response to Salmonella Enteritidis Infection in Chickens.” 2010. Web. 27 Feb 2021.
Vancouver:
Chiang H. Genomic Approaches to Study Innate Immune Response to Salmonella Enteritidis Infection in Chickens. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-12-244.
Council of Science Editors:
Chiang H. Genomic Approaches to Study Innate Immune Response to Salmonella Enteritidis Infection in Chickens. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-12-244

Texas A&M University
10.
Vega, Everardo.
Attachment and survival of viruses on lettuce (Lactuca sativa L. var. capitata L.): role of physicochemical and biotic factors.
Degree: PhD, Food Science and Technology, 2006, Texas A&M University
URL: http://hdl.handle.net/1969.1/4158
► Enteric viruses are responsible for a significant amount of foodborne disease in the United States. Foodborne disease associated with enteric viruses has been increasing within…
(more)
▼ Enteric viruses are responsible for a significant amount of foodborne disease in
the United States. Foodborne disease associated with enteric viruses has been
increasing within the last few years due to technological advances and raised
awareness. Salads and salad crops are the principal vector for transmission of
enteric viruses. The objective of this study was to determine if viruses are able to
attach non-specifically to the surface of lettuce and to determine the forces
responsible for non-specific viral adsorption to lettuce. Additionally, the impact
of the microbial flora on viral persistence was studied to determine the effect on
viruses. The four viruses studied were echovirus 11, feline calicivirus, MS2 and
ÃÂX174. The viruses were chosen based on their varying isoelectric points and
similar physicochemical attributes. The isoelectric point was not the main factor
determining virus attachment to lettuce. Viruses had varying attachment
efficiencies, with echovirus 11 having the highest affinity to lettuce and ÃÂX174 the
least. Viral adsorption to lettuce was mediated by electrostatic forces due to the
removal of virus adsorption at pH 7 and 8 with the addition of 1
M NaCl to the
buffer solutions. Microcosm studies indicated that the microbial flora did not have a negative impact on virus survival. The bacteriophages had the highest
survival rate. Virus survival in the microcosm studies was not indicative of virus
survival on the surface of the lettuce. The animal viruses exhibited survival rates
greater than or equal to the survival of bacteriophages at 4ð C, but at room
temperature viable animal viruses rapidly declined compared to the
bacteriophages. Additional studies also indicated that the microbial flora was
not able to degrade the viruses for aerobic microbial growth. Overall, these
results indicate that viruses are able to attach to the surface of lettuce, providing
a possible explanation for the high incidence of virus associated disease
involving salads and fresh produce. More importantly the use of surrogates for
virus studies involving fresh produce must be re-evaluated, because of the lack of
correlation between animal viruses and bacteriophages. Appropriate viral
surrogates, if used, have to be carefully chosen based on viral physicochemical
properties as well as the infectious route of the virus.
Advisors/Committee Members: Pillai, Suresh D. (advisor), Berghman, Luc R. (committee member), Collisson, Ellen W. (committee member), Zhou, Huaijun (committee member).
Subjects/Keywords: food safety; virus
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Vega, E. (2006). Attachment and survival of viruses on lettuce (Lactuca sativa L. var. capitata L.): role of physicochemical and biotic factors. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4158
Chicago Manual of Style (16th Edition):
Vega, Everardo. “Attachment and survival of viruses on lettuce (Lactuca sativa L. var. capitata L.): role of physicochemical and biotic factors.” 2006. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021.
http://hdl.handle.net/1969.1/4158.
MLA Handbook (7th Edition):
Vega, Everardo. “Attachment and survival of viruses on lettuce (Lactuca sativa L. var. capitata L.): role of physicochemical and biotic factors.” 2006. Web. 27 Feb 2021.
Vancouver:
Vega E. Attachment and survival of viruses on lettuce (Lactuca sativa L. var. capitata L.): role of physicochemical and biotic factors. [Internet] [Doctoral dissertation]. Texas A&M University; 2006. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1969.1/4158.
Council of Science Editors:
Vega E. Attachment and survival of viruses on lettuce (Lactuca sativa L. var. capitata L.): role of physicochemical and biotic factors. [Doctoral Dissertation]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/4158
.