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You searched for +publisher:"Texas A&M University" +contributor:("Templeton, Joe W."). Showing records 1 – 2 of 2 total matches.

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Texas A&M University

1. Seabury, Christopher Mark. Genetic evaluation of the ovine and bovine prion protein genes (PRNP).

Degree: PhD, Genetics, 2006, Texas A&M University

Transmissible spongiform encephalopathies (TSEs), or prion diseases, are a group of inevitably fatal neurodegenerative diseases that occur in mammalian species. Ovine susceptibility to scrapie, the prototypical TSE, is predominantly modulated by nonsynonymous polymorphisms within exon 3 of the ovine prion protein gene (PRNP). Investigation of PRNP exon 3 for two hair-sheep breeds revealed a novel predicted amino acid substitution (P116) associated with the ovine ARQ allele (P116A136R154Q171). Additionally, two novel ovine PRNP genotypes (PARQ/ARR; PARQ/ARQ) also were detected, and most of the hair sheep sampled possessed PRNP exon 3 genotypes associated with some degree of resistance to scrapie and/or experimental BSE (bovine spongiform encephalopathy). Unlike sheep, expression of bovine spongiform encephalopathy (BSE) in cattle and other bovids has not been associated with nucleotide variation within bovine PRNP exon 3. However, BSE susceptibility has been tentatively associated with specific insertion-deletion (indel) polymorphisms within the putative bovine PRNP promoter, and to a lesser extent intron 1, for a few German cattle breeds. Evaluation of the patterns of nucleotide variation associated with bovine PRNP exon 3 provided evidence that strong purifying selection has intensely constrained bovine exon 3 over the long-term evolutionary history of the subfamily Bovinae, as well as evidence for significant purifying selection in regions of bovine PRNP exon 3 that are considered to be of functional, structural, and pathogenic importance in other mammalian species. Evaluation of the frequencies of known indel polymorphisms within the putative bovine PRNP promoter for a panel of U. S. cattle sires revealed no significant differences in the distribution of promoter alleles and/or genotypes between U. S. cattle sires and BSEaffected German cattle. Notably, a nonsynonymous PRNP exon 3 polymorphism (T50C) identified in American bison (Bison bison) was tentatively associated with Brucella spp. seropositivity. Specifically, a significant overabundance (P = 0.021) of Yellowstone National Park bison possessing the CC genotype were Brucella spp. seropositive. Furthermore, the T-allele and TT genotype were observed at significantly higher frequencies in three bison populations that were either founded from Brucella spp. seronegative stock or previously subjected to test-and-slaughter management to eradicate brucellosis. Advisors/Committee Members: Derr, James N. (advisor), Womack, James E. (committee member), Templeton, Joe W. (committee member), Honeycutt, Rodney L. (committee member).

Subjects/Keywords: PRNP; BSE; prion; evolution

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APA (6th Edition):

Seabury, C. M. (2006). Genetic evaluation of the ovine and bovine prion protein genes (PRNP). (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/3333

Chicago Manual of Style (16th Edition):

Seabury, Christopher Mark. “Genetic evaluation of the ovine and bovine prion protein genes (PRNP).” 2006. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021. http://hdl.handle.net/1969.1/3333.

MLA Handbook (7th Edition):

Seabury, Christopher Mark. “Genetic evaluation of the ovine and bovine prion protein genes (PRNP).” 2006. Web. 27 Feb 2021.

Vancouver:

Seabury CM. Genetic evaluation of the ovine and bovine prion protein genes (PRNP). [Internet] [Doctoral dissertation]. Texas A&M University; 2006. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1969.1/3333.

Council of Science Editors:

Seabury CM. Genetic evaluation of the ovine and bovine prion protein genes (PRNP). [Doctoral Dissertation]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/3333


Texas A&M University

2. Schutta, Christopher John. Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis.

Degree: PhD, Veterinary Microbiology, 2009, Texas A&M University

Previous analysis of the bovine SLC11A1 complementary DNA (cDNA) failed to identify any nucleotide variations other than a microsatellite length variation within the 3' untranslated region functionally associated with bovine brucellosis. In this study I set out to identify mutations in the genomic complement of the gene that may be associated with resistance or susceptibility to bovine brucellosis, and to determine if the microsatellite length polymorphism in the 3'UTR of bovine SLC11A1 modulates gene expression and subsequent disease resistance in a phase dependent manner. The results of this study demonstrate that there are seventy-five total single nucleotide polymorphic (SNP) sites (excluding indels) located within the bovine genomic SLC11A1 sequence of a Brucella abortus resistant bull and a susceptible cow. Twenty of these SNPs segregated between resistant and susceptible populations, with 3 non-synonymous SNPs significantly associating with resistance or susceptibility to B. abortus infection. An A695G within exon 2 resulted in a histidine (resistant allele) to arginine (susceptible allele) amino acid substitution and was in significant linkage disequilibrium with the previously described 3' untranslated region (UTR) microsatellite length variation associated with brucellosis resistance. A transcriptional element search in the 3' UTR revealed a ETS-domain PU.1 site, an IFN-γ activation site (GAS), an Interferon Consensus Sequence Binding Protein site (ICSBP) and several Initiation Response sites (Inr), suggesting a possible function for this region in regulation of the expression of SLC11A1. A mobility shift assay confirmed sequence-specific DNA-protein interaction within this region. A luciferase reporter assay indicated that the 3'UTR of SLC11A1 could act as a downstream enhancer for expression. Macrophage killing assays with RAW264.7 cells expressing bovine SLC11A1 demonstrated that the microsatellite repeat is functionally associated with the macrophage killing efficiency, but not in a phase-dependent manner, suggesting that these length polymorphisms do not affect the angular orientation between cooperatively binding transcription factors, and leaves the possibility that the 3'UTR microsatellites regulate SLC11A1 transcription through some alternate mechanism, possibly mRNA stability. Advisors/Committee Members: Templeton, Joe W. (advisor), Adams, L. Garry (committee member), Davis, Donald S. (committee member), Derr, James N. (committee member).

Subjects/Keywords: SLC11A1; brucellosis; natural disease resistance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Schutta, C. J. (2009). Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1874

Chicago Manual of Style (16th Edition):

Schutta, Christopher John. “Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis.” 2009. Doctoral Dissertation, Texas A&M University. Accessed February 27, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1874.

MLA Handbook (7th Edition):

Schutta, Christopher John. “Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis.” 2009. Web. 27 Feb 2021.

Vancouver:

Schutta CJ. Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1874.

Council of Science Editors:

Schutta CJ. Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1874

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