+publisher:"Texas A&M University" +contributor:("Riggs, Penny K")

Texas A&M University

1. Shields, Jordan Elizabeth. Characterization of a Gene Abundantly Expressed in Stallion Testis.

Degree: MS, Animal Breeding, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8975

► NMES1 is a gene of unknown function first characterized in 2002. Reduction of the expression of this gene has been implicated in skin tumorigenesis in… (more)

▼ NMES1 is a gene of unknown function first characterized in 2002. Reduction of the expression of this gene has been implicated in skin tumorigenesis in mice. Expression of NMES1 is observed in epithelial tissue but expression in the testis is significantly higher than in epidermis. Because stallion fertility is an economically important trait, we decided to characterize the NMES1 gene in stallions. We screened the CHORI241 library and obtained the full length equine NMES1 genomic sequence by direct sequencing off of clone CH241-11J8. In order to experimentally determine the 5’ and 3’ untranslated regions (UTRs) we conducted RLM-RACE experiments using stallion testis RNA. The equine NMES1 mRNA is 534 nt long and contains 5 exons. Fluorescence in situ hybridization mapped NMES1 to chromosome Eca1q23. In situ experiments to testis tissue sections were inconclusive and yielded no data confirming the physical expression pattern of NMES1 in stallion testis tissue. In order to determine the expression pattern of NMES1 mRNA we conducted qRT-PCR assays on a panel of stallion testis samples from horses with normal and abnormal fertility. We found that expression was variable among both groups, with significantly less expression in some individuals. We also conducted the qRT-PCR assay on a panel of five equine tissues and found that the expression of NMES1 was more than 100-fold greater in testis than in other tissues examined. miR-147b is a miRNA of unknown target found within the 3’ UTR of NMES1. We conducted a miRNA qRT-PCR assay to determine the expression levels in stallion testis samples from fertile and sub-fertile stallions. We observed similar expression among both groups and the ratio of mRNA to miRNA did not appear constant. We also investigated miR-147b expression in a panel of five equine tissues and found that equine spleen had more than 8-fold greater expression than testis. Advisors/Committee Members: Riggs, Penny K. (advisor), Ing, Nancy (committee member), Raudsepp, Terje (committee member), Welsh, Thomas (committee member).

Subjects/Keywords: stallion fertility; NMES1; testis; miR-147b, male fertility

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APA (6^th Edition):

Shields, J. E. (2012). Characterization of a Gene Abundantly Expressed in Stallion Testis. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8975

Chicago Manual of Style (16^th Edition):

Shields, Jordan Elizabeth. “Characterization of a Gene Abundantly Expressed in Stallion Testis.” 2012. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8975.

MLA Handbook (7^th Edition):

Shields, Jordan Elizabeth. “Characterization of a Gene Abundantly Expressed in Stallion Testis.” 2012. Web. 13 Apr 2021.

Vancouver:

Shields JE. Characterization of a Gene Abundantly Expressed in Stallion Testis. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8975.

Council of Science Editors:

Shields JE. Characterization of a Gene Abundantly Expressed in Stallion Testis. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8975

Texas A&M University

2. Fang, Erica. Molecular Studies in Horses with SRY-Positive XY Sex Reversal.

Degree: MS, Genetics, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10580

► Sex determination in mammals is regulated by the sex-determining region on the Y chromosome (SRY); the presence of SRY activates the male developmental pathway and… (more)

▼ Sex determination in mammals is regulated by the sex-determining region on the Y chromosome (SRY); the presence of SRY activates the male developmental pathway and suppresses the gene network necessary for female gonad development. Mutations in sex determination genes lead to various abnormal sexual phenotypes, including sex reversal syndrome in which the genetic and phenotypic sex do not match. Sex reversal syndrome has been reported in humans, mouse, and several domestic species. In horses, SRY-negative XY sex reversal syndrome has been well described and is caused by deletions on the Y chromosome. However, the molecular causes of the SRY-positive condition in horses and other mammals are not known. This research investigated five horses affected with SRY-positive XY sex reversal syndrome. Sequencing of the coding exon region of the SRY gene in the five cases showed 99-100% alignment with the sequences of normal males. Genotyping of two closely related individuals with 46 normal male controls on an equine SNP50 Beadchip identified two statistically significant SNPs in a ~16 Mb region on the long arm of horse chromosome 3 (ECA3q). The region was analyzed using Gene Ontology (GO) and Gene Relationships Across Implicated Loci (GRAIL) to select functionally relevant candidate genes for sequencing. Further analysis of the entire horse genome was done through array comparative genomic hybridization (aCGH), which investigated possible structural rearrangements, such as copy number variants (CNVs). Deletions of olfactory receptor genes were detected on multiple chromosomes and confirmed through quantitative real-time PCR (qPCR). A homozygous deletion on ECA29 in a region containing genes of the aldo-keto reductase gene family, known to play a role in interconverting sex hormones between active forms and inactive forms, was discovered in two sex reversed animals. The findings were confirmed through qPCR and fluorescence in situ hybridization (FISH), and experiments to define the specific breakpoints of the deletion through PCR have been initiated. This research represents the first systematic search in the horse genome for mutations and CNVs related to sex determination. The findings contribute to better understanding of the molecular mechanisms of sex determination in horses and other mammals, including humans. Advisors/Committee Members: Raudsepp, Terje (advisor), Chowdhary, Bhanu P. (advisor), Riggs, Penny K. (committee member).

Subjects/Keywords: Horses; SRY; Sex Reversal; SNP analysis; array comparative genomic hybridization

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APA (6^th Edition):

Fang, E. (2012). Molecular Studies in Horses with SRY-Positive XY Sex Reversal. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10580

Chicago Manual of Style (16^th Edition):

Fang, Erica. “Molecular Studies in Horses with SRY-Positive XY Sex Reversal.” 2012. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10580.

MLA Handbook (7^th Edition):

Fang, Erica. “Molecular Studies in Horses with SRY-Positive XY Sex Reversal.” 2012. Web. 13 Apr 2021.

Vancouver:

Fang E. Molecular Studies in Horses with SRY-Positive XY Sex Reversal. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10580.

Council of Science Editors:

Fang E. Molecular Studies in Horses with SRY-Positive XY Sex Reversal. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10580

Texas A&M University

3. Prause, Macee Robyn. Analysis of Methylation Patterns in Ejaculated Sperm from F1 Nellore-Angus Crossbred Bulls.

Degree: MS, Animal Science, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/159098

► Methylation of the carbon 5 position of cytosine is a common epigenetic mark in vertebrates, and has been associated with male infertility. Methylation can be… (more)

Subjects/Keywords: Epigenetics; DNA Methylation; Cytosine; Bovine; Sperm

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APA (6^th Edition):

Prause, M. R. (2016). Analysis of Methylation Patterns in Ejaculated Sperm from F1 Nellore-Angus Crossbred Bulls. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/159098

Chicago Manual of Style (16^th Edition):

Prause, Macee Robyn. “Analysis of Methylation Patterns in Ejaculated Sperm from F1 Nellore-Angus Crossbred Bulls.” 2016. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/159098.

MLA Handbook (7^th Edition):

Prause, Macee Robyn. “Analysis of Methylation Patterns in Ejaculated Sperm from F1 Nellore-Angus Crossbred Bulls.” 2016. Web. 13 Apr 2021.

Vancouver:

Prause MR. Analysis of Methylation Patterns in Ejaculated Sperm from F1 Nellore-Angus Crossbred Bulls. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/159098.

Council of Science Editors:

Prause MR. Analysis of Methylation Patterns in Ejaculated Sperm from F1 Nellore-Angus Crossbred Bulls. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/159098

Texas A&M University

4. Vitanza, Sarah M. Expression of Candidate Genes for Horn Growth in Early Bovine Development.

Degree: MS, Animal Science, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7604

► Bovine horns develop primarily after birth and the presence or absence of horns is due to a single gene. It has been reported that the… (more)

▼ Bovine horns develop primarily after birth and the presence or absence of horns is due to a single gene. It has been reported that the horn bud appears in the bovine embryo at d 60 of gestation. Our hypothesis is that the gene that determines the presence of horns is expressed in osteoprogenitor cells of the early fetus and will affect the expression of RUNX2, MSX1, MSX2, and/or TWIST1. To test this hypothesis, bovine fetal samples were collected from commercial females at the Caviness Packing Company in Hereford, Texas. Fetuses ranged from d 28 to d 80 of gestation. A survey of the expression of genes from the region on bovine chromosome 1 known to contain the locus that causes horns (IFNAR1 to SOD1), was conducted using qualitative and quantitative RT-PCR, and in situ hybridization. Genes with known roles in osteogenesis and chrondrogenesis (MSX1, TWIST1, RUNX2 and SOX9) were included as positive controls. With the exception of OLIG1, which was only expressed in the brain, all of the genes investigated were expressed in fetal frontal and parietal bones by qualitative RT-PCR. The level of expression of C21orf59, C21orf66, IL10RB, and SFRS15 increased in the frontal bone of horned samples from d 55 to d 70 of gestation. At d 60 of gestation, a change in the shape of the frontal bone was observed, which has been reported to be the developmental stage when the horn bud appears. At this time point, MSX1, TWIST1, RUNX2 and SOX9 were detected in frontal bone, in cells from the osteoblast lineage, as expected. Furthermore, C21orf59, C21orf62, C21or66 and SFRS15 from the polled interval were localized to developing mesenchyme, osteoblasts and/or osteoclasts of the frontal bone, suggesting that each of these genes has a role in intramembranous bone formation. In addition, gradients of expressed C21orf66 and SFRS15 were detected in developing endochondral bone. There was evidence of an antisense transcript of C21orf66 expressed in the same cell types as the sense transcript. Further characterization of this antisense transcript demonstrated that it covered the entire sense transcript. Based on observed expression in the mesenchyme, rather than just in mature osteoblasts or osteoclasts, C21orf66 and/or its antisense transcript become the most likely candidates for the polled locus. Advisors/Committee Members: Gill, Clare A. (advisor), Riggs, Penny K. (advisor), Sanders, James O. (committee member).

Subjects/Keywords: Bovine; Horns; Polled; Intramembranous bone; Fetal development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vitanza, S. M. (2011). Expression of Candidate Genes for Horn Growth in Early Bovine Development. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7604

Chicago Manual of Style (16^th Edition):

Vitanza, Sarah M. “Expression of Candidate Genes for Horn Growth in Early Bovine Development.” 2011. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7604.

MLA Handbook (7^th Edition):

Vitanza, Sarah M. “Expression of Candidate Genes for Horn Growth in Early Bovine Development.” 2011. Web. 13 Apr 2021.

Vancouver:

Vitanza SM. Expression of Candidate Genes for Horn Growth in Early Bovine Development. [Internet] [Masters thesis]. Texas A&M University; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7604.

Council of Science Editors:

Vitanza SM. Expression of Candidate Genes for Horn Growth in Early Bovine Development. [Masters Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7604

Texas A&M University

5. Courtney, Sean M. Genetic Regulation of Intrinsic Endurance Exercise Capacity in Mice.

Degree: PhD, Kinesiology, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151206

► Endurance exercise capacity is a powerful predictor of health status. Having low levels of endurance exercise capacity has been linked with cardiovascular disease. Variation in… (more)

▼ Endurance exercise capacity is a powerful predictor of health status. Having low levels of endurance exercise capacity has been linked with cardiovascular disease. Variation in endurance exercise capacity, measured during a graded exercise test, has been reported across cross-section, twin, and family studies. This variation is evidence of a genetic component to the phenotype of endurance exercise capacity: however, the genetic factors responsible for explaining this variation are undefined, in part because previous research has been performed on a limited scale. Therefore, three sets of experiments were designed to identify: 1) Novel quantitative trait loci (QTL) for endurance exercise capacity in 34 strains of inbred mice using genome-wide association mapping. 2) The effect of chromosome substitution on endurance exercise capacity using linkage analysis in F2 mice. 3) The effect of chromosome substitution on endurance exercise capacity using wild-derived mice. The main findings of this dissertation are: 1) There are strain-specific differences in endurance exercise capacity across 34 strains of male inbred mice. Genome-wide association mapping identified novel putative QTL on chromosomes 2, 7, 11, and 13. 2) Linkage analysis identified a novel QTL on chromosome 14 at the 56 cM position for run time and work. Linkage analysis also identified a potential sex-specific QTL, with the identified QTL significant for male mice only. 3) Novel putative QTL were identified on chromosomes 3 and 14 in chromosome substitution mice from wild-derived mice. These data suggest that chromosome 14 is an important contributor to the genetic regulation of intrinsic endurance exercise capacity. These studies support a genetic component to endurance exercise capacity by identifying strain-specific differences and novel, putative QTL. Advisors/Committee Members: Massett, Michael P (advisor), Woodman, Christopher R (committee member), Fluckey, James D (committee member), Riggs, Penny K (committee member).

Subjects/Keywords: Endurance exercise; QTL; chromosome substitution

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Courtney, S. M. (2013). Genetic Regulation of Intrinsic Endurance Exercise Capacity in Mice. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151206

Chicago Manual of Style (16^th Edition):

Courtney, Sean M. “Genetic Regulation of Intrinsic Endurance Exercise Capacity in Mice.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/151206.

MLA Handbook (7^th Edition):

Courtney, Sean M. “Genetic Regulation of Intrinsic Endurance Exercise Capacity in Mice.” 2013. Web. 13 Apr 2021.

Vancouver:

Courtney SM. Genetic Regulation of Intrinsic Endurance Exercise Capacity in Mice. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/151206.

Council of Science Editors:

Courtney SM. Genetic Regulation of Intrinsic Endurance Exercise Capacity in Mice. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151206

Texas A&M University

6. Cardin, Jessica Marie. Evaluation of Gene Breed Type and Expression of Feed Efficiency Candidate Genes, and Their Associations with Carcass Traits in F2 Nellore-Angus Steers.

Degree: MS, Animal Breeding, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10123

► Steers produced in Cycle I of the Texas A & M University McGregor Genomics Project (n = 232, F2 Nellore-Angus) were evaluated for carcass composition,… (more)

▼ Steers produced in Cycle I of the Texas A & M University McGregor Genomics Project (n = 232, F2 Nellore-Angus) were evaluated for carcass composition, visceral organ weights, and model predicted residual consumption (MPRC), a measure of feed efficiency. Hot carcass weight was strongly correlated with liver weight (r = 0.70, P < 0.001) heart weight (r = 0.58, P < 0.001), and viscera weight (r = 0.55, P < 0.001) but not spleen weight (r = -0.01, P = 0.83). Liver, heart and viscera weights were moderately positively correlated with external and kidney pelvic heart fat (KPH), but not with marbling. None of the organ weights were correlated with MPRC. A subset of 54 animals was selected for extreme values of residuals of MPRC after a mixed model analysis that included fixed effects of sire and family nested within sire, and these animals were evaluated for insulin-like growth factor I (IGFI) expression in liver samples collected at harvest through quantitative real time polymerase chain reaction (qRT-PCR). IGFI relative quantity (IGFI RQ) was collected from qRT-PCR and was correlated with liver (r = -0.23, P = 0.09), spleen (r = 0.48, P <0.001) and viscera weight (r = 0.24, P = 0.08), but not any carcass trait or MPRC residual. Gene breed types were determined for 4 candidate genes of feed efficiency: insulin like growth factor-1 (IGF1), leptin (LEP), neuropeptide-Y (NPY) and ghrelin (GHRL). Gene breed types were represented as AA, AN, NA, and NN where A and N denote Angus and Nellore, respectively, and paternal inheritance is listed first. Given that contemporary group significantly influenced most traits (MPRC, all carcass traits, liver weight and viscera weight), effects of gene breed type and contemporary group were evaluated together in analyses. GHRL influenced liver (P = 0.02) and viscera weight (P = 0.02), IGF1 influenced kidney, pelvic, and heart fat (P = 0.05), NPY influenced liver weight (P = 0.03) and hot carcass weight (P = 0.04), and LEP influenced ribeye area (P = 0.05) and hot carcass weight (P = 0.04) with a tendency to influence liver weight (P = 0.06). Alternate heterozygotes for GHRL were statistically different in liver weight and viscera weight where NA was heavier than AN. Alternate heterozygotes for LEP did not differ in ribeye area, hot carcass weight, or liver weight. Alternate heterozygotes for NPY differed in liver weight (NA heavier than AN), but did not differ in hot carcass weight. Carcasses with AA for LEP and NPY were 18 to 19 kg heavier (P < 0.05) than those with NN, with heterozygotes intermediate. Advisors/Committee Members: Riggs, Penny K. (advisor), Herring, Andy D. (advisor), Sanders, James O. (committee member), Gill, Clare (committee member).

Subjects/Keywords: organ; efficiency; Angus; Nellore; carcass

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APA (6^th Edition):

Cardin, J. M. (2011). Evaluation of Gene Breed Type and Expression of Feed Efficiency Candidate Genes, and Their Associations with Carcass Traits in F2 Nellore-Angus Steers. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10123

Chicago Manual of Style (16^th Edition):

Cardin, Jessica Marie. “Evaluation of Gene Breed Type and Expression of Feed Efficiency Candidate Genes, and Their Associations with Carcass Traits in F2 Nellore-Angus Steers.” 2011. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10123.

MLA Handbook (7^th Edition):

Cardin, Jessica Marie. “Evaluation of Gene Breed Type and Expression of Feed Efficiency Candidate Genes, and Their Associations with Carcass Traits in F2 Nellore-Angus Steers.” 2011. Web. 13 Apr 2021.

Vancouver:

Cardin JM. Evaluation of Gene Breed Type and Expression of Feed Efficiency Candidate Genes, and Their Associations with Carcass Traits in F2 Nellore-Angus Steers. [Internet] [Masters thesis]. Texas A&M University; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10123.

Council of Science Editors:

Cardin JM. Evaluation of Gene Breed Type and Expression of Feed Efficiency Candidate Genes, and Their Associations with Carcass Traits in F2 Nellore-Angus Steers. [Masters Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10123

Texas A&M University

7. Wang, Jixin. Genome-wide Transcriptome Analysis of Laminar Tissue During the Early Stages of Experimentally Induced Equine Laminitis.

Degree: PhD, Biomedical Sciences, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8718

► Equine laminitis is a debilitating disease that causes extreme sufferring in afflicted horses and often results in a lifetime of chronic pain. The exact sequence… (more)

▼ Equine laminitis is a debilitating disease that causes extreme sufferring in afflicted horses and often results in a lifetime of chronic pain. The exact sequence of pathophysiological events culminating in laminitis has not yet been characterized, and this is reflected in the lack of any consistently effective therapeutic strategy. For these reasons, we used a newly developed 21,000 element equine-specific whole-genome oligoarray to perform transcriptomic analysis on laminar tissue from horses with experimentally induced models of laminitis: carbohydrate overload (CHO), hyperinsulinaemia (HI), and oligofructose (OF). Samples were collected during the developmental (DEV) and Obel grade 1 (OG1) stages of laminitis for the CHO model. For the HI model, samples were collected at the Obel grade 2 (OG2) stage. For the OF model, samples were collected at the 12 h and 24 h time points. Appropriate control samples were obtained for all models. This is the first genome-wide transcriptome analysis of laminar tissue using an equine 21,000 70-mer long oligoarray approach in CHO, HI and OF induced laminitis. Overall, we identified the differential expression of genes encoding S100 calcium binding proteins, extracellular matrix proteins, glycoproteins, transporters, olfactory receptors, genes involved in signal transduction, body‟s homeostasis, apoptosis, and immune response. Between CHO and OF models of laminitis, there were more shared genes. We discovered several common differentially expressed genes (i.e., ADAMTS1, CYCS and CXCL14) among all three models that are likely important to the pathogenesis of equine laminitis. We also discovered what appear to be central roles of apoptosis, inflammatory response, and intracellular ion homeostasis molecular processes in CHO and OF models of laminitis. Pathway analysis detected the NOD-like receptor signaling pathway, which is involved in recognition of intracellular bacteria in both the CHO and OF models of laminitis. Genetic network analysis indicated convergent pathway core molecules present in equine acute laminitis: p38 MAPK and NF-κB. Most importantly, our results of overexpression of anti-microbial genes (i.e., DEFB4, PI3, and CXCL14) suggest the central involvement of these genes in the progression of early equine laminitis and will allow refinement of current hypotheses of disease pathogenesis. Advisors/Committee Members: Chowdhary, Bhanu P. (advisor), Raudsepp, Terje (committee member), Samollow, Paul B. (committee member), Skow, Loren C. (committee member), Riggs, Penny K. (committee member).

Subjects/Keywords: Equine laminitis; microarray; horse; inflammation; functional genomics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, J. (2012). Genome-wide Transcriptome Analysis of Laminar Tissue During the Early Stages of Experimentally Induced Equine Laminitis. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8718

Chicago Manual of Style (16^th Edition):

Wang, Jixin. “Genome-wide Transcriptome Analysis of Laminar Tissue During the Early Stages of Experimentally Induced Equine Laminitis.” 2012. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8718.

MLA Handbook (7^th Edition):

Wang, Jixin. “Genome-wide Transcriptome Analysis of Laminar Tissue During the Early Stages of Experimentally Induced Equine Laminitis.” 2012. Web. 13 Apr 2021.

Vancouver:

Wang J. Genome-wide Transcriptome Analysis of Laminar Tissue During the Early Stages of Experimentally Induced Equine Laminitis. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8718.

Council of Science Editors:

Wang J. Genome-wide Transcriptome Analysis of Laminar Tissue During the Early Stages of Experimentally Induced Equine Laminitis. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8718

Texas A&M University

8. Kim, Seung Kyum. Genetic Regulation of Intrinsic Endothelial Function and Endothelial Responses to Exercise Training.

Degree: PhD, Kinesiology, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/156421

► Endothelial dysfunction is a fundamental component of cardiovascular disease. Exercise training is known to prevent/improve endothelial dysfunction. However, the genetic basis for endothelial function is… (more)

▼ Endothelial dysfunction is a fundamental component of cardiovascular disease. Exercise training is known to prevent/improve endothelial dysfunction. However, the genetic basis for endothelial function is yet to be fully elucidated and the genetic contribution to endothelial responses to exercise training is largely unknown. The purposes of this research were 1) to identify quantitative trait loci (QTL)/candidate genes residing in the QTL responsible for intrinsic endothelial function and 2) to determine the interaction between genetic background and training intensity on the endothelial adaptations to exercise training. In the first study, vasoreactivity was assessed in aortic rings of male mice from 27 inbred strains. Strain-dependent differences were found for vasoreactivity including responses to ACh. Genome-wide association study for responses to ACh revealed four significant and several suggestive QTL, most of which are regions of shared synteny for cardiovascular traits in rats and/or humans. In the second study, a strain survey for the effect of traditional exercise training on vasoreactivity was performed in aortic rings of male mice from 20 inbred strains. Traditional exercise training had subtle effects on vasoreactivity including responses to ACh. Based on the strain survey, four inbred mouse strains (129S1, B6, SJL, and NON) were chosen to examine endothelial responses to two different training intensities [high (HIT) vs. moderate intensity (MOD)]. There was a significant interaction between mouse strain and training intensity on responses to ACh after exercise training. The transcriptional activation of endothelial genes was also influenced by the interaction. There was little overlap between genes altered by HIT and MOD. HIT was associated with pathways for inflammatory responses, while NON MOD genes showed enrichment for vessel growth pathways. In conclusion, the present findings provide strong evidence that genetic background influences endothelial function and its responses to exercise training. Several QTL/candidate genes are suggested as new targets for elucidating the genetic basis of intrinsic endothelial function. Exercise training has non-uniform effects on endothelial function and transcriptional activation of endothelial genes depending on the interaction between genetic background and training intensity. Advisors/Committee Members: Massett, Michael P (advisor), Woodman, Christopher R (committee member), Riggs, Penny K (committee member), Lightfoot, J Timothy (committee member).

Subjects/Keywords: Endothelial function; Genome-wide association study; Quantitative trait loci; Candidate genes; Exercise training; Training Intensity; Gene expression profiling; Pathway analysis

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APA (6^th Edition):

Kim, S. K. (2015). Genetic Regulation of Intrinsic Endothelial Function and Endothelial Responses to Exercise Training. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156421

Chicago Manual of Style (16^th Edition):

Kim, Seung Kyum. “Genetic Regulation of Intrinsic Endothelial Function and Endothelial Responses to Exercise Training.” 2015. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/156421.

MLA Handbook (7^th Edition):

Kim, Seung Kyum. “Genetic Regulation of Intrinsic Endothelial Function and Endothelial Responses to Exercise Training.” 2015. Web. 13 Apr 2021.

Vancouver:

Kim SK. Genetic Regulation of Intrinsic Endothelial Function and Endothelial Responses to Exercise Training. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/156421.

Council of Science Editors:

Kim SK. Genetic Regulation of Intrinsic Endothelial Function and Endothelial Responses to Exercise Training. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156421

Texas A&M University

9. Vaughn, Robert Neil. Genetic Mechanisms that Contribute to Differences in Beef Tenderness Following Electrical Stimulation.

Degree: PhD, Genetics, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/186976

► Tenderness is a key issue for consumers that is influenced by many environmental and genetic factors. Electrical stimulation (ES) is a postmortem treatment used to… (more)

▼ Tenderness is a key issue for consumers that is influenced by many environmental and genetic factors. Electrical stimulation (ES) is a postmortem treatment used to increase tenderness and reduce variation between carcasses within scientific treatment groups. The purpose of this study was to examine genetic factors that influence tenderness in response to ES. Samples were obtained from a crossbred Nellore-Angus herd produced in McGregor TX. Muscle samples were obtained immediately after slaughter, and those selected for this study were based on divergent response to ES. Tenderness was determined by objective Warner-Bratzler shear force measurements following 14 d of aging. A commercial cattle microarray was used to identify large sets of genes with significantly different gene expression between tenderness groups. These data were used to find significantly enriched genetic signaling pathways. A subset of genes based on expression and pathway analysis was selected for verification by realtime quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). A subset of gene products was selected for western blot protein expression assays. In addition SNP haplotype blocks (hapblocks) were constructed that encompassed genes of interest to determine whether parent or breed of origin for these genes contributed to ES response. The ECM and closely related focal adhesion pathways were significantly enriched from the microarray assay. From this pathway a total of 40 genes was assayed by qRT-PCR. Of the assayed genes, many integrins were significantly upregulated in the group that responded well to electrical stimulation compared to the group that responded poorly. Through hapblock analysis, it was found that breed and parent of origin played a role in many production features including efficiency and growth rates. Breed of origin of integrin alpha-6 (ITGA6) could be linked to a 0.15 kg difference in ES residual tenderness values when inherited maternally (P = 0.03). The gene FN1 had a breed of origin effect, with a difference in ES residual tenderness of 0.23 kg for different paternally-inherited hapblocks. Additionally, ITGA6 protein expression was found to closely follow mRNA expression in a subset of animals chosen for western blot analysis. These results suggest that components of the ECM may be a novel area of research for improving tenderness that will benefit both producers and consumers. Advisors/Committee Members: Riggs, Penny K (advisor), Gill, Clare A (committee member), Herring, Andy D (committee member), Smith, Stephen B (committee member).

Subjects/Keywords: Beef; tenderness; electrical stimulation; WBSF

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APA (6^th Edition):

Vaughn, R. N. (2013). Genetic Mechanisms that Contribute to Differences in Beef Tenderness Following Electrical Stimulation. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/186976

Chicago Manual of Style (16^th Edition):

Vaughn, Robert Neil. “Genetic Mechanisms that Contribute to Differences in Beef Tenderness Following Electrical Stimulation.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/186976.

MLA Handbook (7^th Edition):

Vaughn, Robert Neil. “Genetic Mechanisms that Contribute to Differences in Beef Tenderness Following Electrical Stimulation.” 2013. Web. 13 Apr 2021.

Vancouver:

Vaughn RN. Genetic Mechanisms that Contribute to Differences in Beef Tenderness Following Electrical Stimulation. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/186976.

Council of Science Editors:

Vaughn RN. Genetic Mechanisms that Contribute to Differences in Beef Tenderness Following Electrical Stimulation. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/186976

Texas A&M University

10. Runyan, Chase Anthony. Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge.

Degree: PhD, Animal Science, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151767

► This study investigated the effects of vaccine type, sire, day, threshold rectal temperature status, and their potential interactions on growth, daily feed intake and daily… (more)

▼ This study investigated the effects of vaccine type, sire, day, threshold rectal temperature status, and their potential interactions on growth, daily feed intake and daily feed bunk frequency in response to a standardized Bovine Viral Diarrhea Virus (BVDV) challenge. Yearling, F2 and F3 Nellore-Angus steers (n = 380) from the Texas A&M McGregor Genomics herd were utilized over 4 years, and were stratified by sire over three vaccine groups of modified-live (MLV), killed (KV), and non-vaccinated (NON). Vaccines were used in accordance to label directions, and MLV steers were separated from KV and NON steers for 7 to 10 days to prevent transmission of viral particles. All steers were intranasally challenged with BVDV type 1b strain CA0401186A on day 0 of each year. Clinical signs of illness and feeding behavior data were collected daily, while rectal temperature and weight records were collected at days 0, 3, 7, 10, 14, 28, and 42 post-vaccination. The influence of sire was a significant source of variation as both a main effect or as an interaction term for all response variables analyzed. Vaccine type was a significant source of variation as components of interaction terms; lower (P < 0.05) mean rectal temperature was seen in MLV as compared to KV and NON steers. Variation from sire and vaccine type interaction suggests the potential of matching genetic profiles and vaccine protocols to achieve optimum levels of production measures. Daily feed intake and daily bunk visit frequency tended to decrease through day 7, but these traits should be interpreted separately due to the effects of sire and sire by vaccine type interactions. Higher number of bunk visits did not explain levels of intake within some sire groups and vaccine groups. Lung tissue disruption based on color scores of 3 or 4, on 5-point scale was present in more than 65% of cattle that did not have elevated rectal temperature above 40 °C, the threshold basis for provision of antibiotic treatment. Interactions involving sire, vaccine type or rectal temperature status with other factors in this trial illustrates complexity regarding interpretation of cattle health impacts on production traits. Advisors/Committee Members: Herring, Andy D (advisor), Sawyer, Jason E (committee member), Riggs, Penny K (committee member), Welsh, Thomas H (committee member).

Subjects/Keywords: Cattle; BVD virus challenge; Bos indicus; intake; performance

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APA (6^th Edition):

Runyan, C. A. (2013). Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151767

Chicago Manual of Style (16^th Edition):

Runyan, Chase Anthony. “Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/151767.

MLA Handbook (7^th Edition):

Runyan, Chase Anthony. “Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge.” 2013. Web. 13 Apr 2021.

Vancouver:

Runyan CA. Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/151767.

Council of Science Editors:

Runyan CA. Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151767

Texas A&M University

11. Ghosh, Sharmila. Study of Genomic Copy Number Variation in Equine Health and Disease.

Degree: PhD, Biomedical Sciences, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153432

► This is a study of copy number variations (CNVs) in the horse genome to gain knowledge about the role of CNVs in equine biology, and… (more)

▼ This is a study of copy number variations (CNVs) in the horse genome to gain knowledge about the role of CNVs in equine biology, and their contribution to complex diseases and disorders. We constructed a 400K whole-genome tiling array and applied it for the discovery of CNVs in 38 normal horses of 16 diverse breeds, and the Przewalski horse. Altogether, 258 CNV regions (CNVRs) were identified across all autosomes, chrX, and chrUn. The CNVRs comprised 1.3% of the horse genome with chr12 being most enriched. American Miniature Horses had the highest and American Quarter Horses the lowest number of CNVs in relation to Thoroughbred references. The Przewalski horse was similar to native ponies and draft breeds. About 20% of CNVRs were intergenic, while 80% involved 750 annotated genes with molecular functions predominantly in sensory perception, immunity, and reproduction. The findings were integrated with previous CNV studies in the horse to generate a composite genome-wide dataset of 1476 CNVRs. Of these, 301 CNVRs were shared between studies, while 1174 were novel and require further validation. Integrated data revealed that only 41 out of over 400 breeds of the domestic horse have been analyzed for CNVs, whereas this study added 11 new breeds. The composite CNV dataset served as a foundation for the discovery of variants contributing to Recurrent Airway Obstruction (RAO) and XY disorders of sexual development (DSDs), such as cryptorchidism and XY sex reversal. In 16 RAO affected horses 363 CNVRs were identified, of which 31 were novel and not found in healthy horses. A deletion in SPI2 and SERPINA1 was studied in detail because the genes are involved in respiratory diseases in human. In horses with XY DSDs, over 50 novel CNVRs were identified including deletions of functional interest in the pseudoautosomal region and the ATRX gene. A potentially causative homozygous deletion in chr29 disrupting AKR1C genes with functions in sex hormone metabolism was shared between a cryptorchid and two sex reversal horses. The findings effectively improved the knowledge about CNVs in horses, in health and disease, and generated resources for future studies. Advisors/Committee Members: Raudsepp, Terje (advisor), Cothran, Ernest G (committee member), Riggs, Penny K (committee member), Chowdhary, Bhanu P (committee member), Cai, James (committee member).

Subjects/Keywords: CNV; CNVR; RAO; DSD

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APA (6^th Edition):

Ghosh, S. (2014). Study of Genomic Copy Number Variation in Equine Health and Disease. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153432

Chicago Manual of Style (16^th Edition):

Ghosh, Sharmila. “Study of Genomic Copy Number Variation in Equine Health and Disease.” 2014. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/153432.

MLA Handbook (7^th Edition):

Ghosh, Sharmila. “Study of Genomic Copy Number Variation in Equine Health and Disease.” 2014. Web. 13 Apr 2021.

Vancouver:

Ghosh S. Study of Genomic Copy Number Variation in Equine Health and Disease. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/153432.

Council of Science Editors:

Ghosh S. Study of Genomic Copy Number Variation in Equine Health and Disease. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153432

Texas A&M University

12. Orozco Hernandez, Pilar. Relationships between Beef Postharvest Biochemical Factors and Warner-Bratzler Shear Force.

Degree: MS, Animal Science, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/149933

► Biochemical changes in muscle postmortem have been associated with initial beef tenderness early postmortem, and with improvements in tenderness during postmortem storage, defined as meat… (more)

▼ Biochemical changes in muscle postmortem have been associated with initial beef tenderness early postmortem, and with improvements in tenderness during postmortem storage, defined as meat aging. Differences in the initial contractile state of the sarcomere, the ionic environment of the sarcoplasm including pH, the activity of neutral proteolytic enzymes, and collagen content and solubility have been associated with beef tenderness. In Phase I, steaks from four genetic lines of steers and heifers were used to understand the biochemical differences between tough and tender steaks. The most tender (< 30 N Warner Bratzler shear force (WBS)) and toughest Longissimus steaks (< 30 N WBS) from Angus, Braford, Brangus, and Simbrah heifers and steers were used. For Phase II, samples were obtained from a subset of Santa Cruz yearling heifers selected based of genotypes for tenderness (tough and tender) using a commercial genetic marker. Within genotype for tenderness, each animal was randomly assigned to one of four growth enhancement treatments. The most tender (< 30 N WBS) and toughest Longissimus steaks (< 30 N WBS) were selected for use in this study. In Phase I, tough steaks after 3, 10, and 17d postmortem had higher (P < 0.0005) WBS values than tender steaks. Tender steaks came from carcass with slightly higher (P = 0.008) marbling score and (P = 0.01) Quality grade. Sarcomere length, total and soluble collagen, potassium concentration, and m and µcalpain did not differ (P > 0.05) between tough and tender steaks. Sodium concentration at 10 d was higher (P = 0.03) in tough steaks, but only account for 0.05% of the variation in WBS at 3d. Tender steaks had less (P = 0.04) intact desmin at 24h, but intact desmin was not correlated (P > 0.05) with WBS. In Phase II, tough steaks after 3, 10, and 17d postmortem had higher (P < 0.0001) WBS values than tender steaks. Tender steaks came from carcass with slightly higher (P < 0.03) marbling score and (P = 0.02) Quality grade. Tender teaks were slightly lighter (P = 0.02), with more red (P = 0.02) and yellow (P = 0.007) color, and had slightly lower (P = 0.02) pH, compared with tough steaks. Sarcomere length, total and soluble collagen, sodium and potassium concentration, and m and µcalpain did not differ (P > 0.05) between tough and tender steaks. Tender steaks had less (P < 0.0001) intact desmin at 17d postmortem than tough steaks. Intact desmin at 17d was responsible for 4%, 47%, and 30% of WBS variation after 3, 10, and 17d postmortem, respectively. The slight difference in marbling and quality grade did not account for a significant amount of variation in WBS. However, meat color and pH accounted for variation in shear WBS. Calcium flux may have influenced meat tenderness by activation of calpains and may have altered protein to protein interactions. Results suggested that marbling, µ calpain activity, and desmin degradation, and to a lesser extent pH and meat color contributed to meat tenderness. Advisors/Committee Members: Miller, Rhonda K (advisor), Carstens, Gordon E (committee member), Riggs, Penny K (committee member), Smith, Stephen B (committee member).

Subjects/Keywords: calcium; tenderness; Warner-Bratzler shear force; calpain; collagen; marbling; sarcomere length; desmin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Orozco Hernandez, P. (2013). Relationships between Beef Postharvest Biochemical Factors and Warner-Bratzler Shear Force. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149933

Chicago Manual of Style (16^th Edition):

Orozco Hernandez, Pilar. “Relationships between Beef Postharvest Biochemical Factors and Warner-Bratzler Shear Force.” 2013. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/149933.

MLA Handbook (7^th Edition):

Orozco Hernandez, Pilar. “Relationships between Beef Postharvest Biochemical Factors and Warner-Bratzler Shear Force.” 2013. Web. 13 Apr 2021.

Vancouver:

Orozco Hernandez P. Relationships between Beef Postharvest Biochemical Factors and Warner-Bratzler Shear Force. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/149933.

Council of Science Editors:

Orozco Hernandez P. Relationships between Beef Postharvest Biochemical Factors and Warner-Bratzler Shear Force. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149933

Texas A&M University

13. Adams, Amber. Acclimation of Holstein Calves to Transit Stress: The Integration of Endocrine, Immune, and Behavior Systems.

Degree: PhD, Animal Science, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11786

► Little is known about the adaptation of livestock to repeated transport. This study determined how repeated transport affected calf feed intake, plasma cortisol (CORT), post-transport… (more)

▼ Little is known about the adaptation of livestock to repeated transport. This study determined how repeated transport affected calf feed intake, plasma cortisol (CORT), post-transport behavior, and the expression of immune-related genes. Thirty-six 4-month-old Holstein steer calves were housed in groups of six with each group randomly assigned to either transport (T) or control (C) treatments. The T calves were hauled for 6 h in a 7.3 m x 2.4 m goose-neck trailer, at an average density of 0.87 m2/calf, every 7 d for five consecutive weeks. Individual daily intake was determined using Calan gate feeders. Blood samples were obtained in the trailer or home pen via jugular venipuncture before loading, and after 2, 4, and 6 h of transport. Samples were analyzed for CORT, serotonin, tryptophan, and the gene expression of interleukin-4 (IL-4), interleukin-6 (IL-6), chemokine (C-X-C motif) receptor 2, interleukin-12, toll-like receptor-4, toll-like receptor-2, and 5-hydroxytryptamine receptor 2A in leukocytes. Behavior was recorded for transported calves at 5-min intervals for 1 h after return to their home pens. The C calves had a higher feed intake than T calves overall (P = 0.01), on the day of transport (P = 0.007), and the day after transport (P = 0.02). Pre-transport CORT concentrations did not differ by treatment (P = 0.77) or trial (P = 0.32). However, the T calves had higher response CORT concentrations than C calves during Transport 3 (P = 0.006), Transport 4 (P = 0.001) and Transport 5 (P = 0.02). The T calves had the highest response CORT concentrations after 2 h of transport and the lowest response CORT concentrations after 6 h of transport (P < 0.0001). Treatment did not affect gene expression in leukocytes, however, the expression of IL-4 (P = 0.01) and IL-6 (P = 0.05) was significantly lower after 2 h of transport than any other sampling times. These results suggest conflicting conclusions on whether the calves started to acclimate after being transported five times. However, CORT and gene expression differences occurred in response to the blood sampling regimen, which may provide insight to how calves acclimate during prolonged stress. Advisors/Committee Members: Friend, Ted H. (advisor), Berghman, Luc R. (committee member), Holub, Glenn A. (committee member), Riggs, Penny K. (committee member).

Subjects/Keywords: Behavior; Calves; Feed intake; Gene expression; Immune; Serotonin; Transport stress; Tryptophan

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APA (6^th Edition):

Adams, A. (2012). Acclimation of Holstein Calves to Transit Stress: The Integration of Endocrine, Immune, and Behavior Systems. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11786

Chicago Manual of Style (16^th Edition):

Adams, Amber. “Acclimation of Holstein Calves to Transit Stress: The Integration of Endocrine, Immune, and Behavior Systems.” 2012. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11786.

MLA Handbook (7^th Edition):

Adams, Amber. “Acclimation of Holstein Calves to Transit Stress: The Integration of Endocrine, Immune, and Behavior Systems.” 2012. Web. 13 Apr 2021.

Vancouver:

Adams A. Acclimation of Holstein Calves to Transit Stress: The Integration of Endocrine, Immune, and Behavior Systems. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11786.

Council of Science Editors:

Adams A. Acclimation of Holstein Calves to Transit Stress: The Integration of Endocrine, Immune, and Behavior Systems. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11786

Texas A&M University

14. Ritchie, Lauren E. Diet, Disease State, and the Space Environment Modify the Intestinal Microbiota and Mucosal Environment via Microbiota-directed Alterations in Colonocyte Signalling.

Degree: PhD, Genetics, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151706

► Microbial dysbiosis and toll-like receptor (TLR) signaling play a role in colonic injury and inflammation. Ulcerative colitis and radiation are known to alter microbiota, and… (more)

▼ Microbial dysbiosis and toll-like receptor (TLR) signaling play a role in colonic injury and inflammation. Ulcerative colitis and radiation are known to alter microbiota, and diets containing polyphenols impact bacterial populations. We hypothesized that diet can mitigate dextran sodium sulfate (DSS) colitis (sorghum bran diets containing polyphenols) and space environment-induced alterations (normal iron content) in colonic microbiota and TLR signaling. To test this we utilized two experimental paradigms; DSS-induced colitis (3% DSS, 48-hr, 3 exposures, 2 wk separation), and three models to emulate the space environment: 1) fractionated low linear energy transfer (LET) γ radiation (RAD) (3 Gy) and high Fe diet (IRON) (650 mg/kg), 2) high LET Si particle exposure (50 cGy) and 1/6 G hind limb unloading (HLU), and 3) 13 d spaceflight. Bran diets upregulated proliferation, and repair protein (TFF3 and TGFβ) and short chain fatty acid (SCFA) transporter (Slc16a1 and Slc5a8) expression post-DSS. Diet significantly affected 24-hr fecal butyrate production, with Cellulose and Black bran having numerically higher concentrations. Two predominant phyla were identified, Firmicutes and Bacteroidetes, and this ratio was higher in Cellulose DSS. Post DSS#3 the proportion of Bacteroidales, Clostridiales, and Lactobacillales was reduced compared to post DSS#2 for all diets. Black bran non-DSS rats had the highest richness and diversity. Colonic injury negatively correlated with the proportion of Firmicutes, Actinobacteria, and Lactobacillales, and positively correlated with Unknown and Unclassified groups. Bran diets reduced the severity of epithelial injury, maintained fecal butyrate, and prevented microbial dysbiosis and depletion during DSS-induced colitis. IRON+RAD decreased SCFA concentrations. Low and high LET radiation, HLU, IRON and spaceflight increased Bacteroidetes and decreased Firmicutes. HLU and spaceflight increased Clostridiales and decreased Lactobacillales. RAD and IRON+RAD animals had increased Lactobacillales and significantly lower Clostridiales compared to CON and IRON. TLR9 and IL-6 were downregulated by RAD. TLR4, TFF3 and TGFβ differentially changed with IRON and spaceflight. Microgravity independently affected the microbiota, regardless of radiation energy or dose. Each environmental insult differentially altered the microbiota and mucosal gene expression, with distinct diet, microgravity, and radiation effects observed. Bran diets mitigated deleterious effects of colitis, maintained barrier integrity, and prevented microbiota dysbiosis. Advisors/Committee Members: Turner, Nancy D (advisor), Lupton, Joanne R (committee member), Chapkin, Robert S (committee member), Riggs, Penny K (committee member), Sturino, Joseph M (committee member).

Subjects/Keywords: polyphenols; microbiota; radiation; microgravity; ulcerative colitis

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APA (6^th Edition):

Ritchie, L. E. (2013). Diet, Disease State, and the Space Environment Modify the Intestinal Microbiota and Mucosal Environment via Microbiota-directed Alterations in Colonocyte Signalling. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151706

Chicago Manual of Style (16^th Edition):

Ritchie, Lauren E. “Diet, Disease State, and the Space Environment Modify the Intestinal Microbiota and Mucosal Environment via Microbiota-directed Alterations in Colonocyte Signalling.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/151706.

MLA Handbook (7^th Edition):

Ritchie, Lauren E. “Diet, Disease State, and the Space Environment Modify the Intestinal Microbiota and Mucosal Environment via Microbiota-directed Alterations in Colonocyte Signalling.” 2013. Web. 13 Apr 2021.

Vancouver:

Ritchie LE. Diet, Disease State, and the Space Environment Modify the Intestinal Microbiota and Mucosal Environment via Microbiota-directed Alterations in Colonocyte Signalling. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/151706.

Council of Science Editors:

Ritchie LE. Diet, Disease State, and the Space Environment Modify the Intestinal Microbiota and Mucosal Environment via Microbiota-directed Alterations in Colonocyte Signalling. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151706

15. Flores, Erin Gillenwaters. Characterization of the Bovine Cathelicidin Gene Family.

Degree: PhD, Genetics, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9812

► Cathelicidins (CATHLs) are small, cationic antimicrobial peptides that establish an early innate immune defense against infections in mammals. Beyond their wide spectrum of antimicrobial activity,… (more)

▼ Cathelicidins (CATHLs) are small, cationic antimicrobial peptides that establish an early innate immune defense against infections in mammals. Beyond their wide spectrum of antimicrobial activity, these peptides play important roles in wound repair, chemotactic activity, and apoptosis. Thus, comprehensive characterizing of bovine CATHLs could potentially identify underlying inherited differences in innate immunity and disease resistance in cattle. The purpose of the present study was to verify the placement of the CATHL cluster at the distal end of bovine chromosome 22 (BTA22), identify any single nucleotide polymorphisms (SNPs) and insertion-deletion (indel) polymorphisms within the gene family, explore copy number variation, and investigate the functional impact any of these variants may have in overall bovine innate immunity. A framework radiation hybrid map was constructed with 7 markers screened against the bovine 12,000 rad whole genome RH (12K WG-RH) panel, which when compared to the current genome assembly (Btau_4.0) confirmed current gene order. Comparative sequence analysis for 10 domestic cattle breeds representing both Bos taurus taurus and Bos taurus indicus revealed 60 SNPs, 7 of which were nonsynonymous, and 5 indel mutations. Data from array comparative genomic hybridization (aCGH) between four Angus and four Nelore animals showed a 2-fold increase in copy number of the CATHL4 locus, which was verified by quantitative PCR (qPCR) of genomic DNA. Nelore animals showed an approximate 2-fold increase in the CATHL4 gene. Subsequently, the expression of CATHL4 in Nelore neutrophils exhibited a range of 2- to 5-fold increases in CATHL4 gene expression. Finally, a colorimetric bactericidal assay was performed on the neutrophils of the same Angus and Nelore animals previously genotyped for copy number variations (CNVs). After in vitro challenges to Staphylococcus aureus, Salmonella typhimurium, Mannheimia haemolytica, and Pasteurella multocida, the killing capacity of Nelore neutrophils was approximately 20 percent greater than Angus neutrophils for M. haemolytica and 10 percent greater for P. multocida. Characterization of this antimicrobial gene family is central to developing a firm understanding regarding the effects CATHL variation has with respect to bovine innate immunity. Advisors/Committee Members: Womack, James E. (advisor), Derr, James N. (committee member), Long, Charles R. (committee member), Riggs, Penny K. (committee member).

Subjects/Keywords: antimicrobial peptides; cathelicidins; cattle; genetic variation

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APA (6^th Edition):

Flores, E. G. (2012). Characterization of the Bovine Cathelicidin Gene Family. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9812

Chicago Manual of Style (16^th Edition):

Flores, Erin Gillenwaters. “Characterization of the Bovine Cathelicidin Gene Family.” 2012. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9812.

MLA Handbook (7^th Edition):

Flores, Erin Gillenwaters. “Characterization of the Bovine Cathelicidin Gene Family.” 2012. Web. 13 Apr 2021.

Vancouver:

Flores EG. Characterization of the Bovine Cathelicidin Gene Family. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9812.

Council of Science Editors:

Flores EG. Characterization of the Bovine Cathelicidin Gene Family. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9812

16. Simmons, Matthew Aaron. A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle.

Degree: MS, Animal Breeding, 2019, Texas A&M University

URL: http://hdl.handle.net/1969.1/186234

► Diverse expression of any given trait within a breed is required to protect the breed from the unwanted consequences of selective breeding. Within the cattle… (more)

▼ Diverse expression of any given trait within a breed is required to protect the breed from the unwanted consequences of selective breeding. Within the cattle industry, the fluid state of selective breeding trends, and consumer demand, creates a need for intermediate/moderate type cattle within individual breeds. These cattle have the ability to stabilize a given breed and bring it back from the extreme ends of the popular selection trends. This allows cattle breeders to change the genetics of their cattle, with relative speed, and meet consumer demands. Also, it protects individual breeds from harmful genetic mutations. This is evident in the increase in demand for intermediate cattle herds, like the Trask cattle, during the time period when most of the prominent Hereford breeders had carriers of snorter dwarfism in their herds. For this reason, it is important to preserve intermediate cattle lines like the Trask cattle, which have not conformed to popular cattle breeding trends. The genetic influence of various groups of ancestors on Trask bred bulls in current/recent herds was assessed using Wright’s Relationship Coefficient (Rvxvy), and the inbreeding coefficient (Fvx). Mean inbreeding coefficients of a group of 26 representative bulls from Trask bloodlines were compared to the mean inbreeding coefficient of all cattle in the available pedigree. Mean relatedness of the same 26 bulls with 1) a group of 15 prominent ancestors in the Hereford and Polled Hereford breeds, 2) a group of 30 ancestors that had the most descendants in the pedigree, and 3) a group of 19 prominent Trask line ancestors, was compared to the entire pedigree mean relatedness with the same groups. These comparisons were tested by 1) approximating a beta distribution representing the distribution of relatedness or inbreeding coefficients and testing the mean against that approximated distribution, and 2) employing resampling methods to generate a bootstrapped distribution and compare means to those distributions. These two analysis methods produced slightly different results; the beta P-values resulted in a failure to reject the H0, and the bootstrap resulted in the rejection of the Hv0. This difference highlighted the beta distribution method’s inability to account for the variation that occurs among samples drawn from a given population. The bootstrap resampling method was able to account for this variation because it draws numerous random samples to use in the calculation of the empirical P-values. Results provide a scientific assessment on the genetic influence of the Trask pedigree ancestors on the Trask bred bulls in recent/current herds. Testing against approximated beta (β) distributions may have resulted in type II errors (failure to reject the null hypothesis when it is in fact false). Mean relationship coefficients for the ancestors show the Trask herd ancestors had the closest relationship with the Trask bulls (mean Rxy = 0.208), followed by the top 30 ancestors (mean Rvxvy = 0.150), and then the key breed ancestors (mean Rxy = 0.132).… Advisors/Committee Members: Riggs, Penny K (advisor), Sanders, James O (advisor), Herring, Andy D (committee member), Riley, David G (committee member).

Subjects/Keywords: Linebreeding; Pedigree; Bootstrapping; Hereford; Cattle

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APA (6^th Edition):

Simmons, M. A. (2019). A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/186234

Chicago Manual of Style (16^th Edition):

Simmons, Matthew Aaron. “A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle.” 2019. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/186234.

MLA Handbook (7^th Edition):

Simmons, Matthew Aaron. “A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle.” 2019. Web. 13 Apr 2021.

Vancouver:

Simmons MA. A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle. [Internet] [Masters thesis]. Texas A&M University; 2019. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/186234.

Council of Science Editors:

Simmons MA. A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle. [Masters Thesis]. Texas A&M University; 2019. Available from: http://hdl.handle.net/1969.1/186234

17. Simmons, Matthew Aaron. A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle.

Degree: MS, Animal Breeding, 2019, Texas A&M University

URL: http://hdl.handle.net/1969.1/186194

► Diverse expression of any given trait within a breed is required to protect the breed from the unwanted consequences of selective breeding. Within the cattle… (more)

▼ Diverse expression of any given trait within a breed is required to protect the breed from the unwanted consequences of selective breeding. Within the cattle industry, the fluid state of selective breeding trends, and consumer demand, creates a need for intermediate/moderate type cattle within individual breeds. These cattle have the ability to stabilize a given breed and bring it back from the extreme ends of the popular selection trends. This allows cattle breeders to change the genetics of their cattle, with relative speed, and meet consumer demands. Also, it protects individual breeds from harmful genetic mutations. This is evident in the increase in demand for intermediate cattle herds, like the Trask cattle, during the time period when most of the prominent Hereford breeders had carriers of snorter dwarfism in their herds. For this reason, it is important to preserve intermediate cattle lines like the Trask cattle, which have not conformed to popular cattle breeding trends. The genetic influence of various groups of ancestors on Trask bred bulls in current/recent herds was assessed using Wright’s Relationship Coefficient (Rvxvy), and the inbreeding coefficient (Fvx). Mean inbreeding coefficients of a group of 26 representative bulls from Trask bloodlines were compared to the mean inbreeding coefficient of all cattle in the available pedigree. Mean relatedness of the same 26 bulls with 1) a group of 15 prominent ancestors in the Hereford and Polled Hereford breeds, 2) a group of 30 ancestors that had the most descendants in the pedigree, and 3) a group of 19 prominent Trask line ancestors, was compared to the entire pedigree mean relatedness with the same groups. These comparisons were tested by 1) approximating a beta distribution representing the distribution of relatedness or inbreeding coefficients and testing the mean against that approximated distribution, and 2) employing resampling methods to generate a bootstrapped distribution and compare means to those distributions. These two analysis methods produced slightly different results; the beta P-values resulted in a failure to reject the H0, and the bootstrap resulted in the rejection of the Hv0. This difference highlighted the beta distribution method’s inability to account for the variation that occurs among samples drawn from a given population. The bootstrap resampling method was able to account for this variation because it draws numerous random samples to use in the calculation of the empirical P-values. Results provide a scientific assessment on the genetic influence of the Trask pedigree ancestors on the Trask bred bulls in recent/current herds. Testing against approximated beta (β) distributions may have resulted in type II errors (failure to reject the null hypothesis when it is in fact false). Mean relationship coefficients for the ancestors show the Trask herd ancestors had the closest relationship with the Trask bulls (mean Rxy = 0.208), followed by the top 30 ancestors (mean Rvxvy = 0.150), and then the key breed ancestors (mean Rxy = 0.132).… Advisors/Committee Members: Riggs, Penny K (advisor), Sanders, James O (advisor), Herring, Andy D (committee member), Riley, David G (committee member).

Subjects/Keywords: Linebreeding; Pedigree; Bootstrapping; Hereford; Cattle

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Simmons, M. A. (2019). A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/186194

Chicago Manual of Style (16^th Edition):

Simmons, Matthew Aaron. “A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle.” 2019. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/186194.

MLA Handbook (7^th Edition):

Simmons, Matthew Aaron. “A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle.” 2019. Web. 13 Apr 2021.

Vancouver:

Simmons MA. A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle. [Internet] [Masters thesis]. Texas A&M University; 2019. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/186194.

Council of Science Editors:

Simmons MA. A Quantitative Genetic Analysis of the Ancestry of Neil Trask Line Bred Hereford Cattle. [Masters Thesis]. Texas A&M University; 2019. Available from: http://hdl.handle.net/1969.1/186194

18. Brannan, Jaime Lynette. Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks.

Degree: PhD, Genetics, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151106

► Infestation of cattle by Lone Star ticks, Amblyomma americanum, leads to damage of hides intended for leather, weight loss, infertility, and potentially death of cattle,… (more)

▼ Infestation of cattle by Lone Star ticks, Amblyomma americanum, leads to damage of hides intended for leather, weight loss, infertility, and potentially death of cattle, which contribute to production losses for farmers. Public concerns regarding chemical residues in food and the environment necessitate development of chemical-free alternative tick controls, such as breeding for tick-resistant phenotypes and developing anti-tick vaccines. Thus, the goal of this study was elucidation of mechanisms that mediate immune responses in cattle infested with A. americanum using gene expression techniques. Methods for isolation of total RNA from bovine tick bite-site biopsies and blood leukocytes were optimized to provide RNA suitable for gene expression studies. Tick bite-site biopsies (6 mm) and blood leukocytes were collected from a total of 13 calves (N=6, Group 4 and N=7, Group 5 calves) during experimental tick infestations to determine A. americanum tick-susceptible and -resistant phenotypes. Microarray experiments compared gene expression in tick bite-sites from tick-susceptible, moderately tick-resistant, and highly tick-resistant calves. A total of 35 genes were profiled in tick bite-site biopsies and 12 genes were evaluated in blood leukocytes via gene-specific qRT-PCR assays, and analyzed for each phenotype and for each group of calves as a whole. Analysis of microarray data revealed differential expression of IL-1R-mediators among the three cattle phenotypes. Expression profiles generated by qRT-PCR for TLR-mediating genes such as TLR2, TLR4, CD14, and MyD88 suggest that a MyD88-dependent signaling pathway may mediate the development of acquired immunity in cattle infested with Lone Star ticks. Additionally, increased expression for IL12, IFNgamma, and TNFalpha suggests that a Th1-type cell-mediated reaction may be activated, whereas increased expression of IL6, IL10, and IGHG1 supports the involvement of a Th2-type humoral-mediated response at tick bite-sites in cattle infested with at A. americanum. Regression analyses identified strong correlations between factors involved in pattern recognition in tick bite-site biopsies, including associations between TLR4 and IL1alpha, and between IL1alpha and IL1RN. In conclusion, this dissertation reports optimal methodology for gene expression studies in tick-infested cattle and provides preliminary data concerning the underlying mechanisms associated with the immune response in Lone Star tick-infested cattle. Advisors/Committee Members: Holman, Patricia J (advisor), Womack, James E (advisor), Riggs, Penny K (committee member), Tizard, Ian R (committee member), Pruett, John H (committee member).

Subjects/Keywords: gene expression; qRT-PCR; Amblyomma americanum; tick-resistance; cattle

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APA (6^th Edition):

Brannan, J. L. (2013). Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151106

Chicago Manual of Style (16^th Edition):

Brannan, Jaime Lynette. “Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/151106.

MLA Handbook (7^th Edition):

Brannan, Jaime Lynette. “Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks.” 2013. Web. 13 Apr 2021.

Vancouver:

Brannan JL. Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/151106.

Council of Science Editors:

Brannan JL. Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151106

Texas A&M University

19. Caldwell, Lisa. The Influence of Breed and Temperament on Circulating Concentrations of Insulin-like Growth Factor-I (IGF-I) and Its Relationship to Feed Efficiency in Beef Cattle.

Degree: MS, Physiology of Reproduction, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-734

► Insulin-like growth factor-I (IGF-I) is a growth hormone that acts as a key modulator of the growth axis. Serum and plasma concentrations of IGF-I have… (more)

▼ Insulin-like growth factor-I (IGF-I) is a growth hormone that acts as a key modulator of the growth axis. Serum and plasma concentrations of IGF-I have been linked to economically important traits in beef cattle. In order to determine whether concentrations of IGF-I differed among breeds of beef cattle, plasma samples from purebred and crossbred animals were analyzed. Two calf crops were derived from three-breed diallel matings using temperate and tropically-adapted breeds of cattle. The breeds consisted of temperate Bos taurus (A; Angus), tropical Bos indicus (B; Brahman), and tropical Bos taurus (R; Romosinuano). Plasma samples were obtained from 10 heifers and 10 steers of each breed-type at weaning, and two dates post-weaning. Concentrations of IGF-I were determined by radioimmunoassay (RIA). Breed differences were observed (P < 0.001). Relative to the temperate Bos taurus breed, IGF-I was greater in tropically-adapted breed-types. In an effort to select for the improvement of economically important traits, experiments were performed to explore the possible use of concentration of IGF-I and temperament assessment as tools for selection. Using a Calan gate system, 3 Brahman heifer crops were fed during70-day trials. Performance and feed intake data were collected to determine feed efficiency. Temperament, determined by exit velocity and pen score, was evaluated at weaning. Serum samples were taken at weaning and days 0 and 70 of each trial. Concentrations of IGF-I and cortisol were determined by RIA. Correlations including IGF-I were weak (P > 0.05). Temperament had no significant effect on RFI but may affect ADG. In an attempt to examine the relationship between IGF-I and RFI, body weight and feed intake data were collected during individual finishing phase feeding trials, on steers at El Reno, OK. The breeds consisted of temperate Bos taurus (A; Angus), tropical Bos indicus (B; Brahman), and tropical Bos taurus (R; Romosinuano). Plasma samples were obtained from 10 steers of each breed-type at weaning and days 0 and 60 of each finishing phase. Concentrations of IGF-I were determined by RIA. Correlations between IGF-I and RFI were weak (P > 0.05). Breed and year significantly influenced RFI (P < 0.01). Advisors/Committee Members: Randel, Ronald D. (advisor), Riggs, Penny K. (advisor), Welsh, Thomas H. (committee member), Chase, Chad C. (committee member).

Subjects/Keywords: IGF-I

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Caldwell, L. (2010). The Influence of Breed and Temperament on Circulating Concentrations of Insulin-like Growth Factor-I (IGF-I) and Its Relationship to Feed Efficiency in Beef Cattle. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-734

Chicago Manual of Style (16^th Edition):

Caldwell, Lisa. “The Influence of Breed and Temperament on Circulating Concentrations of Insulin-like Growth Factor-I (IGF-I) and Its Relationship to Feed Efficiency in Beef Cattle.” 2010. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-734.

MLA Handbook (7^th Edition):

Caldwell, Lisa. “The Influence of Breed and Temperament on Circulating Concentrations of Insulin-like Growth Factor-I (IGF-I) and Its Relationship to Feed Efficiency in Beef Cattle.” 2010. Web. 13 Apr 2021.

Vancouver:

Caldwell L. The Influence of Breed and Temperament on Circulating Concentrations of Insulin-like Growth Factor-I (IGF-I) and Its Relationship to Feed Efficiency in Beef Cattle. [Internet] [Masters thesis]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-734.

Council of Science Editors:

Caldwell L. The Influence of Breed and Temperament on Circulating Concentrations of Insulin-like Growth Factor-I (IGF-I) and Its Relationship to Feed Efficiency in Beef Cattle. [Masters Thesis]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-734

Texas A&M University

20. Dickens, Charles. Structural, Functional and Evolutionary Characterization of Sense-Antisense Transcripts in Mammals.

Degree: PhD, Genetics, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-772

► Sense-antisense transcripts (SATs) are messenger RNA (mRNA) transcripts that have regions that are complementary to regions of other mRNA transcripts. SATs may play an influential… (more)

Subjects/Keywords: bioinformatics; sense-antisense

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dickens, C. (2010). Structural, Functional and Evolutionary Characterization of Sense-Antisense Transcripts in Mammals. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-772

Chicago Manual of Style (16^th Edition):

Dickens, Charles. “Structural, Functional and Evolutionary Characterization of Sense-Antisense Transcripts in Mammals.” 2010. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-772.

MLA Handbook (7^th Edition):

Dickens, Charles. “Structural, Functional and Evolutionary Characterization of Sense-Antisense Transcripts in Mammals.” 2010. Web. 13 Apr 2021.

Vancouver:

Dickens C. Structural, Functional and Evolutionary Characterization of Sense-Antisense Transcripts in Mammals. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-772.

Council of Science Editors:

Dickens C. Structural, Functional and Evolutionary Characterization of Sense-Antisense Transcripts in Mammals. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-772

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