+publisher:"Texas A&M University" +contributor:("Magill, Clint")

Texas A&M University

1. Urriola Simons, Jazmina Itzel. Biotechnological Approaches for Genetic Improvement of Sorghum.

Degree: PhD, Molecular and Environmental Plant Sciences, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151264

► Sorghum (Sorghum bicolor L., Moench) is the fifth most important cereal crop in the world and represents an important source of food, feed and energy… (more)

▼ Sorghum (Sorghum bicolor L., Moench) is the fifth most important cereal crop in the world and represents an important source of food, feed and energy in several countries. Recently, there has been an increasing interest in sorghum cultivation worldwide, since it is relatively more drought- and heat-tolerant than other cereal crops, and it is better suited for the predicted consequences of global warming. In Africa and Asia, sorghum is primarily used as food for more than 500 million people, while in the Americas and Australia, it is used mainly as a maize-substitute in livestock feed. In the United States, sorghum is also being used in the production of ethanol. In view of its diverse utility, sorghum offers a large number of target traits that could be modified to meet the required applications. In this work, we have used different genetic engineering approaches to address two important issues in sorghum: seed quality and nitrogen use efficiency. First, we examined the temporal and spatial activity of a rice glutelin gene (GluA-2) promoter, in transgenic sorghum. Results from quantitative and histochemical GUS assays, as well as from transcript analyses, showed that this promoter is highly active during the middle stages of sorghum seed development and that it controls transgene expression specifically in the seed endosperm. This means that the GluA-2 promoter can serve as a useful tool in introducing novel traits into sorghum seed in order to improve the quality of this important cereal. Furthermore, we investigated the effects of cytosolic glutamine synthetase (GS1) and alanine aminotransferase (AlaAT) gene overexpression on nitrogen metabolism and plant growth in sorghum. T_(2) generation plants transformed with a sorghum GS1 gene (Gln1) driven by the maize ubiquitin promoter exhibited enhanced grain yield and biomass accumulation under optimal nitrogen levels. Advisors/Committee Members: Rathore, Keerti S. (advisor), Kolomiets, Michael (committee member), Loopstra, Carol (committee member), Magill, Clint (committee member).

Subjects/Keywords: Biotechnology; sorghum

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APA (6^th Edition):

Urriola Simons, J. I. (2013). Biotechnological Approaches for Genetic Improvement of Sorghum. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151264

Chicago Manual of Style (16^th Edition):

Urriola Simons, Jazmina Itzel. “Biotechnological Approaches for Genetic Improvement of Sorghum.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/151264.

MLA Handbook (7^th Edition):

Urriola Simons, Jazmina Itzel. “Biotechnological Approaches for Genetic Improvement of Sorghum.” 2013. Web. 13 Apr 2021.

Vancouver:

Urriola Simons JI. Biotechnological Approaches for Genetic Improvement of Sorghum. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/151264.

Council of Science Editors:

Urriola Simons JI. Biotechnological Approaches for Genetic Improvement of Sorghum. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151264

Texas A&M University

2. Ahn, Ezekiel Jin Sung. Host Defense Responses of Sorghum and Johnsongrass Against Colletotrichum sublineola and Identification of Candidate Defense Related Genes in Sorghum.

Degree: PhD, Plant Pathology, 2020, Texas A&M University

URL: http://hdl.handle.net/1969.1/191515

► While sorghum (Sorghum bicolor L. Moench.) is the fifth most important cereal grown worldwide, johnsongrass (Sorghum halepense L. Pers.) is a creeping perennial weed that… (more)

▼ While sorghum (Sorghum bicolor L. Moench.) is the fifth most important cereal grown worldwide, johnsongrass (Sorghum halepense L. Pers.) is a creeping perennial weed that interferes with crop productivity. Due to genetic similarity to sorghum, johnsongrass is considered to have potential as an alternate source of pathogen resistance genes for sorghum. In order to test this hypothesis, three isolates of Colletotrichum sublineola P. Henn., which causes sorghum anthracnose, were inoculated onto twenty-six johnsongrass cultivars collected from across the southern U.S. by using an excised leaf method. Each C. sublineola isolate caused various degrees of infection when inoculated onto different johnsongrass cultivars. Moreover, three different C. sublineola isolates caused different responses on the same johnsongrass cultivar. Expression of early defense response related genes, including β-1,3-glucanase, chalcone synthase 8 (CHS8), pathogen induced chitinase, flavonoid-3’-hydroxylase, pathogenesis related protein-10 (PR-10), and thaumatin-like protein, were measured 24 hrs and 48 hrs post inoculation in selected johnsongrass cultivars by Real-Time qRT-PCR. The results revealed that levels of defense responses varied among cultivars but were not sufficient to establish a basis for resistance. When the same johnsongrass cultivars were inoculated in a greenhouse study with conidia of C. sublineola isolate FSP53 from sorghum, some showed evidence of a hypersensitive response. However, successful reproduction of the pathogen as detected by formation of acervuli and setae was seen only on SH1116 and on only one leaf of this cultivar. In addition, C. sublineola isolates were inoculated on leaf blades and midribs of the johnsongrass cultivars along with two sorghum cultivars, BTx623 and SC748-5 by using an excised leaf method. Some johnsongrass cultivars and BTx623 showed different responses between the two tissues. Based on the results from Real-time qRT-PCR, host resistance gene expression in leaf blades and midribs showed three different patterns in BTx623 inoculated with FSP53 isolate of C. sublineola. Finally, a sorghum mini core collection was scored over several years for response to Colletotrichum sublineola, Peronosclerospora sorghi, and Sporisorium reilianum, the causal agents of anthracnose, downy mildew, and head smut, respectively. The screening results were combined with over 290,000 Single nucleotides polymorphic (SNP) loci from an updated version of a publicly available genotype by sequencing (GBS) dataset available for the mini core collection. GAPIT (Genome Association and Prediction Integrated Tool) R package was used to identify chromosomal locations that differ in disease response. When the top scoring SNPs were mapped to the most recent version of the published sorghum genome, in each case, a nearby and most often the closest annotated gene has precedence for a role in host defense. Advisors/Committee Members: Magill, Clint (advisor), Bagavathian, Muthukumar (committee member), Prom, Louis K (committee member), Odvody, Gary (committee member).

Subjects/Keywords: plant pathology; sorghum; johnsongrass; anthracnose; colletotrichum sublineola

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APA (6^th Edition):

Ahn, E. J. S. (2020). Host Defense Responses of Sorghum and Johnsongrass Against Colletotrichum sublineola and Identification of Candidate Defense Related Genes in Sorghum. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/191515

Chicago Manual of Style (16^th Edition):

Ahn, Ezekiel Jin Sung. “Host Defense Responses of Sorghum and Johnsongrass Against Colletotrichum sublineola and Identification of Candidate Defense Related Genes in Sorghum.” 2020. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/191515.

MLA Handbook (7^th Edition):

Ahn, Ezekiel Jin Sung. “Host Defense Responses of Sorghum and Johnsongrass Against Colletotrichum sublineola and Identification of Candidate Defense Related Genes in Sorghum.” 2020. Web. 13 Apr 2021.

Vancouver:

Ahn EJS. Host Defense Responses of Sorghum and Johnsongrass Against Colletotrichum sublineola and Identification of Candidate Defense Related Genes in Sorghum. [Internet] [Doctoral dissertation]. Texas A&M University; 2020. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/191515.

Council of Science Editors:

Ahn EJS. Host Defense Responses of Sorghum and Johnsongrass Against Colletotrichum sublineola and Identification of Candidate Defense Related Genes in Sorghum. [Doctoral Dissertation]. Texas A&M University; 2020. Available from: http://hdl.handle.net/1969.1/191515

Texas A&M University

3. Chaudhary, Priyanka Revansiddha. Postharvest Treatment Effects on Health Promoting Properties and Flavonoid Genes of Grapefruit.

Degree: PhD, Horticulture, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/161599

► Certain naturally occurring compounds present in grapefruit have shown several health promoting properties such as anticancer, antioxidant, antimicrobial and cardioprotective. However, the levels of these… (more)

▼ Certain naturally occurring compounds present in grapefruit have shown several health promoting properties such as anticancer, antioxidant, antimicrobial and cardioprotective. However, the levels of these naturally occurring compounds are influenced by several preharvest and postharvest factors. The primary objective of the current research is to determine the effects of different postharvest treatments on naturally occurring compounds present in grapefruit. In the first and second studies, effect of ethylene degreening on ‘Star Ruby’ and ‘Rio Red’ grapefruit natural compounds was investigated. Degreening helped to improve grapefruit color while maintaining the health promoting compounds. Significant influence of ethylene treatment was observed on flavonoids and furocoumarins. The third and fourth study examined the changes in ‘Star Ruby’ and ‘Rio Red’ grapefruit under cold storage and with low temperature conditioning treatment. Conditioning the fruits prior to cold storage reduced the incidence of chilling injury and conditioned fruits had similar or higher levels of health promoting compounds compared to fruits stored under cold storage without conditioning. However, results suggest that for short term storage of few weeks, storing fruits at 11⁰C was better for retention of most naturally occurring compounds. The fifth study focused on use of modified atmosphere packaging (MAP) to maintain grapefruit quality and nutritional properties. Two MAP films, micro-perforated (modified oxygen, carbon dioxide and humidity levels) and macro-perforated (modified humidity only), were investigated for their influence on naturally occurring compounds and fruit quality under a prolonged storage period. MAP treatments did not have significant effect on ascorbic acid, limonoids and fruit quality parameters such as total soluble solids, acidity, fruit taste, decay and disorders. Based on our research, use of MAP films is recommended to maintain fruit quality and health promoting compounds. The sixth and seventh studies focused on variation in health promoting compounds, specifically, flavonoid pathway gene expression and volatile compounds present in ‘Rio Red’ grapefruit during fruit development and maturity. Overall expression of flavonoid pathway genes and related flavonoid content decreased as the fruits developed and matured, with the levels being highest in immature fruits harvested in June. Levels of limonene decreased as the fruits developed from June to April; while, nootkatone levels increased with fruit development and maturity. The eighth study investigated the effect of different ethylene concentrations on flavonoid pathway gene expression and related flavonoids in grapefruit. Significant effect of ethylene concentration was observed on flavonoids and furocoumarins as well as the genes involved in the flavonoid biosynthesis. Overall, significant influence of different postharvest treatments was observed on grapefruit health promoting compounds. We believe that this research will be helpful to the citrus industry… Advisors/Committee Members: Patil, Bhimanagouda S (advisor), Jayaprakasha, G. K. (committee member), Lombardini, Leonardo (committee member), Awika, Joseph M (committee member), Magill, Clint (committee member).

Subjects/Keywords: Grapefruit; ethylene degreening; low temperature conditioning; modified atmosphere packaging; maturity stages; vitamin C; carotenoids; limonoids; flavonoids; furocoumarins; volatiles; gene expression.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chaudhary, P. R. (2017). Postharvest Treatment Effects on Health Promoting Properties and Flavonoid Genes of Grapefruit. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161599

Chicago Manual of Style (16^th Edition):

Chaudhary, Priyanka Revansiddha. “Postharvest Treatment Effects on Health Promoting Properties and Flavonoid Genes of Grapefruit.” 2017. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/161599.

MLA Handbook (7^th Edition):

Chaudhary, Priyanka Revansiddha. “Postharvest Treatment Effects on Health Promoting Properties and Flavonoid Genes of Grapefruit.” 2017. Web. 13 Apr 2021.

Vancouver:

Chaudhary PR. Postharvest Treatment Effects on Health Promoting Properties and Flavonoid Genes of Grapefruit. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/161599.

Council of Science Editors:

Chaudhary PR. Postharvest Treatment Effects on Health Promoting Properties and Flavonoid Genes of Grapefruit. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161599

Texas A&M University

4. Zhang, Huan. Characterization of Fsr1-Interacting Complex and Its Downstream Pathogenic Subnetwork Modules in Fusarium verticillioides.

Degree: PhD, Plant Pathology, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/187260

► Fusarium verticillioides is an ascomycete fungus responsible for stalk and ear rots of maize. Previously, we identified a striatin-like protein Fsr1 that plays a key… (more)

▼ Fusarium verticillioides is an ascomycete fungus responsible for stalk and ear rots of maize. Previously, we identified a striatin-like protein Fsr1 that plays a key role in stalk rot pathogenesis. In mammals, striatin interacts with multiple proteins to form a STRIPAK (striatin-interacting phosphatase and kinase) complex that regulates a variety of developmental processes and cellular mechanisms. In this study, we identified the homolog of a key mammalian STRIPAK component STRIP1/2 in F. verticillioides, FvStp1, that interacts with Fsr1 in vivo. Gene deletion analysis showed that FvStp1 is critical for F. verticillioides stalk rot virulence. In addition, we identified three proteins, designated FvCyp1, FvScp1 and FvSel1, that interact with the Fsr1 CC domain by yeast-two-hybrid screen. Importantly, FvCyp1, FvScp1, and FvSel1 co-localize to endomembrane structures, each having preferred localization in the cell, and they are all required for F. verticillioides virulence in stalk rot. Moreover, these proteins are necessary for proper localization of Fsr1 to endoplasmic reticulum (ER) and nuclear envelope. To further characterize genetic networks downstream of Fsr1, we performed RNA-Seq with maize B73 stalks inoculated with wild type and fsr1 mutant. We used a computationally efficient branch-out technique, along with an adopted probabilistic pathway activity inference method, to identify functional subnetwork modules likely involved in F. verticillioides virulence. We identified two putative hub genes, i.e., FvSYN1 and FvEBP1 identified from the potential virulence-associated subnetwork modules for functional validation and network robustness studies, such as gene knockout, virulence assays and qPCR studies. Our results provide evidence that FvSYN1 and FvEBP1 are important virulence genes that can infulence the expression of closely correlated genes, providing evidence that these are important hub genes of their respective subnetworks. Further characterization of FvSYN1 showed that FvSyn1 is important for regulating spore germination and hyphal morphology. Furthermore, FvSyn1 is localized to vacuoles, plasma membranes, and septa, and has been shown to play a role in the response to cell wall stressors. Motif-deletion studies showed that both N-terminal SynN domain and C-terminal SNARE domain of FvSyn1 are required for pathogenicity but dispensable for fumonisin production and sexual mating. Advisors/Committee Members: Shim, Won-Bo (advisor), He, Ping (committee member), Kolomiets, Mikhailo V (committee member), Magill, Clint (committee member).

Subjects/Keywords: Fungal pathogen; maize disease; Fusarium; striatin; protein interaction; computational analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, H. (2017). Characterization of Fsr1-Interacting Complex and Its Downstream Pathogenic Subnetwork Modules in Fusarium verticillioides. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/187260

Chicago Manual of Style (16^th Edition):

Zhang, Huan. “Characterization of Fsr1-Interacting Complex and Its Downstream Pathogenic Subnetwork Modules in Fusarium verticillioides.” 2017. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/187260.

MLA Handbook (7^th Edition):

Zhang, Huan. “Characterization of Fsr1-Interacting Complex and Its Downstream Pathogenic Subnetwork Modules in Fusarium verticillioides.” 2017. Web. 13 Apr 2021.

Vancouver:

Zhang H. Characterization of Fsr1-Interacting Complex and Its Downstream Pathogenic Subnetwork Modules in Fusarium verticillioides. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/187260.

Council of Science Editors:

Zhang H. Characterization of Fsr1-Interacting Complex and Its Downstream Pathogenic Subnetwork Modules in Fusarium verticillioides. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/187260

Texas A&M University

5. Radwan, Ghada Lotfy Hassan Elhefny. Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance.

Degree: PhD, Plant Pathology, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/149318

► Sporisorium reilianum is a basidiomycetous fungus that causes head smut in sorghum and maize. Infection requires the formation of a dikaryon between spores of compatible… (more)

▼ Sporisorium reilianum is a basidiomycetous fungus that causes head smut in sorghum and maize. Infection requires the formation of a dikaryon between spores of compatible mating type and leads to a change from yeast-like to hyphal growth within the host plant. This switch is controlled through mating type loci. Among forty four different sorghum isolates of S. reilianum (SRS), including five different pathotypes, only three compatible pairs were detected, leading to the establishment of haploid cultures with three different “a locus” mating types, as verified by mRNA expression. Amino acid sequence comparison showed that the pheromone gene components of the a mating type loci of sorghum isolates (SRS) are highly similar to those of maize isolates (SRZ). Genetic diversity was measured using amplified fragment length polymorphism (AFLP) to compare isolates collected from the same and different hosts. AFLP analysis showed polymorphism both within and between SRS and SRZ isolates, with isolates from each host tending to show greater similarity. However, the study did not reveal patterns that could be associated with pathotype of sorghum isolates. A recent outbreak of sorghum downy mildew in Texas has led to the discovery of both metalaxyl fungicide resistance and a new pathotype, P6, in the causal organism, Peronosclerospora sorghi. To identify sources of resistance against P6, a total of 336 sorghum lines (245 mini-core lines from ICRISAT, India, 67 elite accessions from KSU, Kansas and 24 accessions from Texas) were used in a greenhouse study. Fifty two mini-core and 20 accessions from Kansas and 13 from Texas were recorded with ≤10% infection and characterized as resistant for further confirmation. Out of the 52 resistant mini-core accessions, 20 were photo-insensitive and showed 0 % infection. Eleven out of 20 from Kansas showed 0% infection. To quantify the genetic diversity among resistant accessions a high throughput ABI Prism 3100 DNA sequencing system was used for DNA fingerprinting with 60 Simple Sequence Repeat (SSR) markers representing all 10 sorghum linkage groups. Analysis of SSR patterns showed high diversity among the resistant sorghum accessions that were collected from different geographical regions and include the five defined races of Sorghum bicolor. Accessions that are not closely related were most likely to represent unique sources of genetic resistance to P. sorghi. Advisors/Committee Members: Magill, Clint W (advisor), Starr , James (committee member), Isakeit , Thomas (committee member), Johnston, J. Spencer (committee member).

Subjects/Keywords: Peronosclerospora sorghi; Sporisorium reilianum; Genetic diversity; Head smut; Downy mildew

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APA (6^th Edition):

Radwan, G. L. H. E. (2013). Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149318

Chicago Manual of Style (16^th Edition):

Radwan, Ghada Lotfy Hassan Elhefny. “Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance.” 2013. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/149318.

MLA Handbook (7^th Edition):

Radwan, Ghada Lotfy Hassan Elhefny. “Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance.” 2013. Web. 13 Apr 2021.

Vancouver:

Radwan GLHE. Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/149318.

Council of Science Editors:

Radwan GLHE. Molecular Comparison and DNA Fingerprinting of Sporisorium reilianum and Peronosclerospora sorghi Relating to Host Specificity and Host Resistance. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149318

Texas A&M University

6. McLachlan, Juanita. A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus.

Degree: MS, Genetics, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/149490

► Two Tomato bushy stunt virus (TBSV) proteins, P19 and P22, have been found to interact with the Nicotiana benthamiana host proteins Hin19 and HFi22 in… (more)

▼ Two Tomato bushy stunt virus (TBSV) proteins, P19 and P22, have been found to interact with the Nicotiana benthamiana host proteins Hin19 and HFi22 in yeast two,hybrid assays. To determine functional roles of these interacting host proteins, viral induced gene silencing (VIGS) was employed to knock,down their expression. TBSV has been demonstrated to activate a virus,specific antiviral response pathway in N. benthamiana. To characterize this pathway, the antiviral RNAi induced silencing complex (RISC) was isolated from TBSV-infected plants. Additionally, putative RISC-associated proteins were identified in silico and suggested roles for these have been identified through literature and database searches. A further aim was the identification of proteins that coimmunoprecipitate with the TBSV-induced RISC following RISC isolation. A primary aim of this investigation was to identify functional roles for host proteins that interact with the two TBSV 3-terminal encoded proteins, P22 and P19. Each of these has functional roles in viral movement and pathogenicity. In yeast two-hybrid assays, P22 has been shown to interact with HFi22 while P19 interacts with Hin19. VIGS was utilized in attempts to silence the expression of these two host proteins in order to determine their functional roles. VIGS-mediated suppression of the TBSV-interacting proteins Hin19 and HFi22 has not been accomplished. Despite multiple attempts and multiple approaches, these proteins have not been amenable to silencing. In light of this finding, it is proposed that rather than utilizing VIGS to down-regulate protein levels for Hin19 and HFi22, other approaches should be utilized. To characterize the TBSV-mediated RNAi pathway, functionally active antiviral RISC was purified from TBSV-infected N. benthamiana plants using ion-exchange chromatography. This RISC was found to be active only in the degradation of TBSV transcripts, indicating the specificity expected from a programmed RISC. Characterization and identification of proteins that copurify with RISC has not yet been accomplished, though in silico analysis has yielded over 150 putative RISC-associated proteins. Of these, a subset has been identified as highly likely candidates based upon function and/or homology to RISC-associated proteins in non-plant organisms, and a model for the TBSV-induced antiviral pathway has been proposed. Advisors/Committee Members: Scholthof, Herman B. (advisor), Scholthof, Karen-Beth (committee member), Magill, Clint (committee member), Zhang, Xiuren (committee member).

Subjects/Keywords: TBSV; Tomato bushy stunt virus; RNAi; vRISC

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McLachlan, J. (2013). A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149490

Chicago Manual of Style (16^th Edition):

McLachlan, Juanita. “A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus.” 2013. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/149490.

MLA Handbook (7^th Edition):

McLachlan, Juanita. “A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus.” 2013. Web. 13 Apr 2021.

Vancouver:

McLachlan J. A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/149490.

Council of Science Editors:

McLachlan J. A Study of Nicotiana Benthamiana Protein Interactions with Tomato Bushy Stunt Virus. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149490

Texas A&M University

7. Zhao, Cheng. Metabolic Engineering to Enhance Terpene Yield in Nicotiana Tabacum.

Degree: PhD, Plant Pathology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/174000

► Terpenoids provide a diverse range of applications in fuels, chemicals, specialty materials, nutraceuticals, and pharmaceuticals. Terpenes also provide unconventional sinks for the carbon fixed by… (more)

▼ Terpenoids provide a diverse range of applications in fuels, chemicals, specialty materials, nutraceuticals, and pharmaceuticals. Terpenes also provide unconventional sinks for the carbon fixed by photosynthesis. However, it is highly challenging to increase terpene production in higher plants, partially due to downstream conversion, toxicity to the cell and feedback regulations. My thesis research has mainly focused on developing strategies to address the challenges of terpene production in Nicotiana tabacum. First, a co-compartmentation strategy was design and implemented, in which biosynthesis and storage were co-compartmentalized via a synthetic droplet as an effective new strategy to improve the bioproduct yield, with squalene as a model compound. Instability of squalene was demonstrated by mathematic modeling and dark treatment experiments. Thus, a hydrophobic protein was designed and introduced into the tobacco chloroplast to generate a synthetic droplet for terpene storage. Simultaneously, squalene biosynthesis enzymes were introduced to chloroplasts together with the droplet-forming protein to co-compartmentalize the biosynthesis and storage of squalene. The strategy has enabled a significant increase of squalene yield without compromising plant growth. Confocal fluorescent microscopy imaging, Stimulated Raman Scattering microscopy, and droplet composition analysis confirmed the formation of synthetic storage droplets in chloroplasts. Second, an inter-compartmental ‘pull and block’ strategy was established to re-balance squalene biosynthesis and degradation via silencing squalene epoxidases. An actively expressed squalene epoxidase SQE3 was identified in the leaf of N. tabacum. An artificial microRNA was designed to target the consensus coding sequence of SQE3 to reduce the downstream conversion of squalene. Simultaneously, the squalene biosynthesis pathway was over-expressed in N. tabacum chloroplasts. Suppression of SQE3 was confirmed by RT-PCR. Squalene conversion decrease in SQE3-suppressed plants was confirmed by measuring sterol levels by GC-MS. The squalene yield in SQE3 suppressed plants has achieved 3.98 mg/g fresh weight without comprising plant growth. Overall, these studies established efficient photosynthetic platforms for terpene production. A sufficient sink capacity and a minimized downstream conversion was emphasized in the studies for high yield of bioproducts. The technology advances also provided new strategies for stabilizing high value bioproducts. Advisors/Committee Members: Yuan, Joshua S (advisor), Dickman, Martin B (committee member), Devarenne, Timothy (committee member), Magill, Clint W (committee member).

Subjects/Keywords: Squalene

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, C. (2018). Metabolic Engineering to Enhance Terpene Yield in Nicotiana Tabacum. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/174000

Chicago Manual of Style (16^th Edition):

Zhao, Cheng. “Metabolic Engineering to Enhance Terpene Yield in Nicotiana Tabacum.” 2018. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/174000.

MLA Handbook (7^th Edition):

Zhao, Cheng. “Metabolic Engineering to Enhance Terpene Yield in Nicotiana Tabacum.” 2018. Web. 13 Apr 2021.

Vancouver:

Zhao C. Metabolic Engineering to Enhance Terpene Yield in Nicotiana Tabacum. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/174000.

Council of Science Editors:

Zhao C. Metabolic Engineering to Enhance Terpene Yield in Nicotiana Tabacum. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/174000

8. Shin, Joonhee. Characterization of PP2A regulatory B subunits in Fusarium verticillioides.

Degree: MS, Plant Pathology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-8041

► Fusarium verticillioides is a pathogen of maize causing ear rot and stalk rot. The fungus also produces fumonisins, a group of mycotoxins linked to disorders… (more)

Subjects/Keywords: Fusarium verticillioides; filamentous fungus; fumonisin; PP2A

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APA (6^th Edition):

Shin, J. (2012). Characterization of PP2A regulatory B subunits in Fusarium verticillioides. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-8041

Chicago Manual of Style (16^th Edition):

Shin, Joonhee. “Characterization of PP2A regulatory B subunits in Fusarium verticillioides.” 2012. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-8041.

MLA Handbook (7^th Edition):

Shin, Joonhee. “Characterization of PP2A regulatory B subunits in Fusarium verticillioides.” 2012. Web. 13 Apr 2021.

Vancouver:

Shin J. Characterization of PP2A regulatory B subunits in Fusarium verticillioides. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-8041.

Council of Science Editors:

Shin J. Characterization of PP2A regulatory B subunits in Fusarium verticillioides. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-8041

9. Morris, James Larry. Characterization of plant cation/h+ antiporters and how they can impact nutrition.

Degree: PhD, Molecular and Enviromental Plant Sciences, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2553

► Calcium transporters regulate calcium fluxes within cells. Plants, like all organisms, contain channels, pumps and exchangers to carefully modulate intracellular calcium levels. Numerous Arabidopsis proteins… (more)

▼ Calcium transporters regulate calcium fluxes within cells. Plants, like all organisms, contain channels, pumps and exchangers to carefully modulate intracellular calcium levels. Numerous Arabidopsis proteins have been characterized which can transport calcium. However, there are numerous genes whose products have not been fully characterized. One method I used to infer function was to analyze various promoter lengths of 17 putative cation transporters fused to reporter and to observe changes in the reporter expression in response to various stimuli. Using a more in depth approach I set out to characterize the function of AtCCX3. Here I show Arabidopsis AtCCX3 can suppress yeast mutants defective in vacuolar Na+ and K+ transport. AtCCX3-expressing yeast cells conferred Mn2+ sensitivity when highly expressed. Functional epitope tagged AtCCX3 fusion proteins were localized to endomembranes in plants and yeast. Expression of AtCCX3 increased in plants treated with NaCl, KCl and MnCl2 and caused increased Na+ accumulation and increased K+ transport. Ectopic expression of AtCCX3 in tobacco produced lesions in the leaves, stunted growth, resulted in the accumulation of higher levels of numerous cations and increased protein oxidation preceded alterations in leaf growth. These characteristics define AtCCX3 as an endomembrane localized transporter with biochemical properties distinct from previously characterized plant transporters. In the remaining studies I used the mouse model to determine how molecular changes to plants can improve the nutritional quality of the food. Previously, the cod5 M. truncatula mutant was identified which contains identical calcium concentrations to wild-type, but contains no oxalate crystals. Mice fed intrinsically labeled cod5 plants had 22.87% (p<0.001) calcium absorption compared to wild type plants. In a second study, using mice I examined if increased expression of a calcium transporter which increases calcium concentration 2-fold, alters bioavailable calcium. In mice feeding regimes (n = 120), I measured 45Ca-incorporation into bones, and determined that mice required twice the serving size of control carrots to obtain the calcium found in sCAX1-carrots. Together, these two studies demonstrate how the potential utility of removing calcium absorption inhibitors and fortifying vegetables can improve calcium bioavailability. Advisors/Committee Members: Binzel, Marla (advisor), Hirschi, Kendal H. (advisor), Magill, Clint (committee member), Pike, Leonard (committee member).

Subjects/Keywords: caclium; transport; bioavailibility; oxalate

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APA (6^th Edition):

Morris, J. L. (2009). Characterization of plant cation/h+ antiporters and how they can impact nutrition. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2553

Chicago Manual of Style (16^th Edition):

Morris, James Larry. “Characterization of plant cation/h+ antiporters and how they can impact nutrition.” 2009. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2553.

MLA Handbook (7^th Edition):

Morris, James Larry. “Characterization of plant cation/h+ antiporters and how they can impact nutrition.” 2009. Web. 13 Apr 2021.

Vancouver:

Morris JL. Characterization of plant cation/h+ antiporters and how they can impact nutrition. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2553.

Council of Science Editors:

Morris JL. Characterization of plant cation/h+ antiporters and how they can impact nutrition. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2553

10. Katile, Seriba Ousmane. Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene.

Degree: PhD, Plant Pathology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2114

► Sorghum grain mold and anthracnose are two major diseases of sorghum (Sorghum bicolor) that constrain sorghum production worldwide. Grain mold is caused by several species… (more)

▼ Sorghum grain mold and anthracnose are two major diseases of sorghum (Sorghum bicolor) that constrain sorghum production worldwide. Grain mold is caused by several species of fungi, but the two most common are Curvularia lunata and Fusarium thapsinum. Isolates of these two species were used to inoculate panicles of selected sorghum cultivars in green house and field experimentations. Panicles were sprayed at the time of anthesis with conidial suspensions of the two fungal species individually or in a mixture and with water to serve as a control. Samples were collected 48 hours after inoculation for RNA extraction. In greenhouse studies, four cultivars (Tx2911, Sureno, SC170 and RTx430) were used while thirteen cultivars were grown in the field experiments. Gene expression was measured for the following genes using real time polymerase chain reactions (rt-PCR): PR10, β-glucanase, chitinase, thaumatin, sormatin, phenyalanine ammonia lyase (PAL), obtusifoliol 14α-demethylase (Obtus), antifungal protein (AFP), apoptosis related protein (Apop) and leucine rich repeat (LRR). Seed germination tests in field grown cultivars indicated that germination rates for SC279-14E, SC660 and Sureno were not greatly influenced by grain mold. Covering the panicles with bags served to protect them against grain mold pathogens. The seed mycoflora test showed that Fusarium thapsinum was the most frequently recovered species and there were more species present in non-covered panicles. The response of sorghum cultivars to grain mold infection involves multiple defense genes. Real time PCR used to study the expression of sorghum defense in greenhouse grown plants showed that mRNA encoding PR-10, a small 10 kDa protein, was highly expressed in the glumes and spikelets of resistant cultivars Tx2911 and Sureno and constitutively in leaves. The expression of some other defense genes like beta-glucanase, chitinase and AFP was variable. Sormatin was not expressed. Expression of β-glucanase, chitinase, and PR10 was higher in field than in greenhouse experiments. A second area of research involved tagging of a resistance gene for sorghum anthracnose. Three AFLP markers (Xtxa607, Xtxa3181 and Xtxa4327) and three SSRs (Xtxp3, Xtxp55 and Xtxp72) were identified. These markers were loosely linked to the resistance genes. The markers are located on linkage group B. The results suggest that markers located 20-30 cM on one side or the other of those tested should provide useful tags for the resistance gene. Advisors/Committee Members: Magill, Clint W. (advisor), Isakeit, Thomas (committee member), Prom, Louis K. (committee member), Rooney, William L. (committee member).

Subjects/Keywords: grain mold; RT-PCR; defense genes; C. lunata; F.thapsinum; C. sublineolum; AFLP; SSR; marker

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APA (6^th Edition):

Katile, S. O. (2009). Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2114

Chicago Manual of Style (16^th Edition):

Katile, Seriba Ousmane. “Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene.” 2009. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2114.

MLA Handbook (7^th Edition):

Katile, Seriba Ousmane. “Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene.” 2009. Web. 13 Apr 2021.

Vancouver:

Katile SO. Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2114.

Council of Science Editors:

Katile SO. Expression of defense genes in sorghum grain mold and tagging and mapping a sorghum anthracnose resistance gene. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2114

11. Baradji, Issa. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.

Degree: PhD, Veterinary Microbiology, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

► Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected… (more)

▼ Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected to play a role in erythrocyte invasion. To investigate interaction between AMA-1 and the host cell, the ectodomain region of the B. microti ama-1 gene was cloned into an expression vector, expressed as a histidine-tagged fusion protein, and used to probe red blood cell membrane proteins in far Western blot assays. The B. microti ama-1 ectodomain, which excludes the signal peptide and the transmembrane region of the open reading frame, was amplified from a cloned gene sequence. The AMA-1 ectodomain is a membrane bound polypeptide that extends into the extracellular space and is most likely to interact or initiate interaction with the host red blood cell surface receptor(s). The amplicon was ligated into a protein expression vector to produce a 58.1 kDa recombinant His-tagged fusion protein, which was confirmed by Western blot analysis. The recombinant B. microti AMA-1 fusion protein was enriched on nickel affinity columns and then used to probe mouse, human and horse red blood cell membrane proteins in far Western blot assays. Babesia microti AMA-1 consistently reacted strongly with a protein migrating at 49 kDa. A similar reaction occurred between the B. microti AMA-1 and horse red blood cell membrane proteins, suggesting that similar interacting proteins of this size are shared by red blood cells from the three species. The B. microti AMA-1 may bind to red blood cell membrane sialic-acid groups, as shown for other Babesia spp. This may explain the signal at the 49 kDa position observed between B. microti AMA-1 and red blood cell membrane proteins from three different species. Further studies may determine if the binding epitopes of the red blood cell binding partner at this position vary and contribute to the specificity of each parasite AMA-1 for their respective host cells. Advisors/Committee Members: Holman, Patricia J. (advisor), Ball, Judith M. (committee member), Magill, Clint C. (committee member), Womack, James E. (committee member).

Subjects/Keywords: Babesia microti; Apical Membrane Antigen-1; AMA-1; erythrocyte; parasite ligand; red blood cell; receptor; protein-protein interaction

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APA (6^th Edition):

Baradji, I. (2010). The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

Chicago Manual of Style (16^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

MLA Handbook (7^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Web. 13 Apr 2021.

Vancouver:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

Council of Science Editors:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

Texas A&M University

12. Byun, Bohyun. Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis.

Degree: PhD, Plant Physiology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2818

► Light is a very important factor affecting every aspect of plant development. Plant developmental responses to light are sensitive to the direction, intensity, color, and… (more)

▼ Light is a very important factor affecting every aspect of plant development. Plant developmental responses to light are sensitive to the direction, intensity, color, and duration of light. Light is perceived by an extensive set of photoreceptors that includes the red/far-red light–absorbing phytochromes and blue/UV-A light–absorbing cryptochromes. The Arabidopsis mutant seedling hyper-responsive to light 6 (shl6) has exaggerated developmental responses to available light. In the low light, shl6 seedlings have a phenotype similar to wild-type plants grown in high light, with short hypocotyls, expanded cotyledons, and well-developed first true leaves. In addition, the roots of shl6 are short and highly branched. The SHL6 gene was mapped to a position on chromosome 5 between simple sequence length polymorphism (SSLP) markers nga249 and nga151. Two cosmid clones from this interval (introduced by Agrobacterium-mediated transformation) complemented the shl6 mutant phenotype. One candidate gene identified by complementation is a member of the glycosyltransferase family. The sequence of shl6 mutant differs from wild type Columbia allele of this gene (At5g13000) by a single nucleotide substitution in the first exon. This putative SHL6 gene encodes a member of a glycan synthase-like (GSL12) gene family that includes callose synthase. The β-1,3-D-glucan callose is found in the cell plate of dividing cells, in pollen mother cell walls, and pollen tubes. Callose synthase and related genes have not been previously implicated in developmental responses to light. We also observed that 90% of Col-0 anthers showed high callose deposition, but shl6 mutant did not display callose deposition in the anthers. The pollen viability in the shl6 was lower than Col-0. The epidermal cell elongation in shl6 hypocotyls was reduced when compared with Col-0. Therefore, we conclude that the mutation in light-regulated SHL6/GSL12 was involved in the synthesis of callose as well as light signaling. Advisors/Committee Members: Pepper, Alan (advisor), Lekven, Arne (committee member), Magill, Clint (committee member), McKnight, Thomas (committee member).

Subjects/Keywords: Keyword1: light-regulated Keyword2: callose

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APA (6^th Edition):

Byun, B. (2009). Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2818

Chicago Manual of Style (16^th Edition):

Byun, Bohyun. “Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis.” 2009. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2818.

MLA Handbook (7^th Edition):

Byun, Bohyun. “Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis.” 2009. Web. 13 Apr 2021.

Vancouver:

Byun B. Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2818.

Council of Science Editors:

Byun B. Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2818

Texas A&M University

13. Roche, Meghan C. A study of programmed cell death in cotton (gosypium hirsutum) fiber.

Degree: MS, Plant Breeding, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1599

► Cotton fiber has been postulated to undergo a process of programmed cell death (PCD) during the maturation phase of development. A parallel may exist between… (more)

Subjects/Keywords: programmed cell death; cotton

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APA (6^th Edition):

Roche, M. C. (2009). A study of programmed cell death in cotton (gosypium hirsutum) fiber. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1599

Chicago Manual of Style (16^th Edition):

Roche, Meghan C. “A study of programmed cell death in cotton (gosypium hirsutum) fiber.” 2009. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1599.

MLA Handbook (7^th Edition):

Roche, Meghan C. “A study of programmed cell death in cotton (gosypium hirsutum) fiber.” 2009. Web. 13 Apr 2021.

Vancouver:

Roche MC. A study of programmed cell death in cotton (gosypium hirsutum) fiber. [Internet] [Masters thesis]. Texas A&M University; 2009. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1599.

Council of Science Editors:

Roche MC. A study of programmed cell death in cotton (gosypium hirsutum) fiber. [Masters Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1599

Texas A&M University

14. Viswanathan, Aparna. Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2.

Degree: MS, Plant Pathology, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/394

► The internal transcribed spacer region (ITS) from fourteen samples of Sclerospora graminicola was amplified using a nested PCR. These regions of the rDNA were amplified… (more)

Subjects/Keywords: Sclerospora graminicola; phylogenetic analysis

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APA (6^th Edition):

Viswanathan, A. (2004). Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/394

Chicago Manual of Style (16^th Edition):

Viswanathan, Aparna. “Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2.” 2004. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/394.

MLA Handbook (7^th Edition):

Viswanathan, Aparna. “Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2.” 2004. Web. 13 Apr 2021.

Vancouver:

Viswanathan A. Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2. [Internet] [Masters thesis]. Texas A&M University; 2004. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/394.

Council of Science Editors:

Viswanathan A. Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2. [Masters Thesis]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/394

Texas A&M University

15. Xie, Xin. Sugar sensing and regulation of conidiation in Neurospora crassa.

Degree: PhD, Genetics, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/1069

► The orange bread mold Neurospora crassa is a useful model for the study of filamentous fungi. One of the asexual reproduction cycles in N. crassa,… (more)

▼ The orange bread mold Neurospora crassa is a useful model for the study of filamentous fungi. One of the asexual reproduction cycles in N. crassa, macroconidiation, can be induced by several environmental cues, including glucose starvation. The rco-3 gene is a regulator of sugar transport and macroconidiation in N. crassa and was proposed to encode a sugar sensor (Madi et al., 1997). To identify genes that are functionally related to RCO-3, three distinct suppressors of the sorbose resistance phenotype of rco-3 were isolated and characterized. The dgr-1 mutant phenotypically resembles rco-3 and may be part of the rco-3 signaling pathway. Epistatic relationship among rco-3, dgr-1 and the suppressors were carried out by analyzing rco-3; dgr-1 and sup; dgr-1 double mutants. These analyses indicate that rco-3 is epistatic to dgr-1. A cDNA microarray containing 1363 N. crassa genes was generated to examine the transcriptional response of wild type cells grown in the presence of glucose or starved for glucose for two hours. Comparing N. crassa profiling data with the published diauxic shift data from S. cerevisiae (DeRisi et al., 1997) revealed that S. cerevisiae and N. crassa share a similar, but not identical, transcriptional response pattern for genes belonging to central carbon metabolism. The microarray results indicate that N. crassa utilizes glucose through fermentation and respiration simultaneously in aerobic culture, a finding that is consistent with previous measurements of ethanol production and enzyme activities in N. crassa. The same microarray was used to examine the transcriptional response to glucose status in rco-3 and dgr-1 mutants. The two mutants display similar expression patterns for most of the genes on the microarray supporting a close functional relationship between them. In addition, I identified a high affinity glucose transport gene in N. crassa, whose transcription is under the control of glucose, rco-3 and dgr-1. Advisors/Committee Members: Ebbole, Daniel (advisor), Magill, Clint (committee member), Bell-Pedersen, Deborah (committee member), Beremand, Marian (committee member).

Subjects/Keywords: sugar sensing; conidiation; carbon repression; Neurospora crassa

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APA (6^th Edition):

Xie, X. (2004). Sugar sensing and regulation of conidiation in Neurospora crassa. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/1069

Chicago Manual of Style (16^th Edition):

Xie, Xin. “Sugar sensing and regulation of conidiation in Neurospora crassa.” 2004. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/1069.

MLA Handbook (7^th Edition):

Xie, Xin. “Sugar sensing and regulation of conidiation in Neurospora crassa.” 2004. Web. 13 Apr 2021.

Vancouver:

Xie X. Sugar sensing and regulation of conidiation in Neurospora crassa. [Internet] [Doctoral dissertation]. Texas A&M University; 2004. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/1069.

Council of Science Editors:

Xie X. Sugar sensing and regulation of conidiation in Neurospora crassa. [Doctoral Dissertation]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/1069

Texas A&M University

16. Filippi, Marta Cristina. Signalling pathway in appressorium formation in Magnaporthe grisea.

Degree: PhD, Plant Pathology, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/1109

► We identified a synthetic hexapeptide that blocks Magnaporthe grisea appressorium formation, in artificial hydrophobic surface. The results suggest that peptides interfere with surface recognition. M.… (more)

Subjects/Keywords: Appressorium formation; Appressorium maturation; Signalling pathway; Magnaporthe grisea; antimicrobial peptide; synthetic peptide

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APA (6^th Edition):

Filippi, M. C. (2004). Signalling pathway in appressorium formation in Magnaporthe grisea. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/1109

Chicago Manual of Style (16^th Edition):

Filippi, Marta Cristina. “Signalling pathway in appressorium formation in Magnaporthe grisea.” 2004. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/1109.

MLA Handbook (7^th Edition):

Filippi, Marta Cristina. “Signalling pathway in appressorium formation in Magnaporthe grisea.” 2004. Web. 13 Apr 2021.

Vancouver:

Filippi MC. Signalling pathway in appressorium formation in Magnaporthe grisea. [Internet] [Doctoral dissertation]. Texas A&M University; 2004. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/1109.

Council of Science Editors:

Filippi MC. Signalling pathway in appressorium formation in Magnaporthe grisea. [Doctoral Dissertation]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/1109

Texas A&M University

17. He, Bin. Techniques for studying the nuclear condition of giant cells induced by Meloidogyne species.

Degree: MS, Plant Pathology, 2006, Texas A&M University

URL: http://hdl.handle.net/1969.1/3243

► Nematodes of the genus Meloidogyne are known as Âroot-knotÂ nematodes due to the characteristic knots or galls found on the roots of infected plants. Root-knot… (more)

▼ Nematodes of the genus Meloidogyne are known as Âroot-knotÂ nematodes due to the characteristic knots or galls found on the roots of infected plants. Root-knot nematodes attack over 2000 species of plants and cause over 80 billion dollars lost annually. Giant cells are feeding sites of root-knot nematodes and are enlarged multinuclear cells induced by the nematodes in susceptible host roots and that function to provide nutrients to the nematode. This thesis presents data on two techniques of studying the nuclear condition in giant cells. Colchicine was used to arrest mitosis in giant cells in a previous study. Here we test the effect of colchicine on nematode activity. The results showed that colchicine did not affect nematode egg hatch, juvenile activity, or hatch of eggs produced by treated juveniles. These results confirm that colchicine can be used to arrest mitosis in giant cells without affecting the nematode parasite. A major obstacle to the study of giant cells is collecting tissue samples that are specific to giant cells. Laser capture microdissection (LCM) is a technique that allows one to sample a single giant cell. A focused laser beam was used to collect samples of giant cell cytoplasm from fixed and sectioned tissues. RNA was then extracted from those isolated samples. Using three tomato genes as test samples, specific primers were designed to measure expression level of Rb7, LHA4, and HXK1 gene by Real-Time PCR. Expression of LHA4 and Rb7 increased with time after inoculation, and immature giant cells reached levels that were 3 and 6 times, respectively, that of cortical cells, but which were not different from root meristem cells. Expression of HXK1 did not change with time after inoculation and has the same level of that in root tip and cortical tissues. These data confirmed that the techniques of LCM coupled with RT-real-time PCR can be used to quantitate expression of genes at different stages of giant cell development without contamination from surrounding cells. Advisors/Committee Members: Starr, James L. (advisor), Magill, Clint (committee member), McKnight, Thomas (committee member).

Subjects/Keywords: giant cell; LCM

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APA (6^th Edition):

He, B. (2006). Techniques for studying the nuclear condition of giant cells induced by Meloidogyne species. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/3243

Chicago Manual of Style (16^th Edition):

He, Bin. “Techniques for studying the nuclear condition of giant cells induced by Meloidogyne species.” 2006. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/3243.

MLA Handbook (7^th Edition):

He, Bin. “Techniques for studying the nuclear condition of giant cells induced by Meloidogyne species.” 2006. Web. 13 Apr 2021.

Vancouver:

He B. Techniques for studying the nuclear condition of giant cells induced by Meloidogyne species. [Internet] [Masters thesis]. Texas A&M University; 2006. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/3243.

Council of Science Editors:

He B. Techniques for studying the nuclear condition of giant cells induced by Meloidogyne species. [Masters Thesis]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/3243

Texas A&M University

18. Zhang, Jinglan. Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays.

Degree: MS, Plant Pathology, 2006, Texas A&M University

URL: http://hdl.handle.net/1969.1/3332

► The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes of the octadecanoid pathway which converts linolenic acid to a phytohormone, jasmonic acid. Bioinformatics analysis of ESTs and… (more)

Subjects/Keywords: genomic; 12-Oxo-Phytodienoic Acid Reductase; maize

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APA (6^th Edition):

Zhang, J. (2006). Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/3332

Chicago Manual of Style (16^th Edition):

Zhang, Jinglan. “Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays.” 2006. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/3332.

MLA Handbook (7^th Edition):

Zhang, Jinglan. “Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays.” 2006. Web. 13 Apr 2021.

Vancouver:

Zhang J. Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays. [Internet] [Masters thesis]. Texas A&M University; 2006. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/3332.

Council of Science Editors:

Zhang J. Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays. [Masters Thesis]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/3332

Texas A&M University

19. Sabry, Ahmed Mohamed-Bashir. QTL mapping of resistance to sorghum downy mildew in maize.

Degree: PhD, Plant Pathology, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/460

► Sorghum downy mildew (SDM) of maize is caused by the oomycete Peronosclerospora sorghi (Weston and Uppal) C. G. Shaw. The disease can cause devastating yield… (more)

▼ Sorghum downy mildew (SDM) of maize is caused by the oomycete Peronosclerospora sorghi (Weston and Uppal) C. G. Shaw. The disease can cause devastating yield losses in maize (Zea mays L.). Quantitative trait loci (QTLs) mediating resistance to SDM were mapped using both restriction fragment length polymorphisms (RFLPs), and simple sequence repeats (SSRs) in 220 F2 individual maize progeny derived from a cross between two extremes; highly susceptible inbred parent SC-TEP5-19-1-3-1-4-1-1 (white) and highly resistant inbred P345C4S2B46-2-2-1-2-B-B-B (yellow). The phenotypic expression was assessed on F2:3 families in a wide range of environments under natural field infection and in a controlled greenhouse screening method. Heritability estimates of disease reaction ranged from 93.3% in Thailand sit 1 to 48% in Thailand sit 2. One hundred and thirty three polymorphic markers were assigned to the ten chromosomes of maize with LOD scores exceeding 4.9 covering about 1265 cM with an average interval length between markers of 9.5 cM. About 90% of the genome was located within a 10 cM distance to the nearest marker. Three putative QTLs were detected in association with resistance to SDM in different environments using composite interval mapping. Despite environmental and symptom differences, one QTL on chromosome 2 bin 9 had a major effect in all trials and explained up to 70% of the phenotypic variation in Thailand where the highest disease pressure was experienced. Two other QTLs on chromosome 3 bin 5 and chromosome 9 bin 2 had a minor effect, each explaining no more than 4% of the phenotypic variation. These results revealed one major gene and two minor genes that control sorghum downy mildew resistance. These markers should be very useful in breeding programs in facilitating the introgression of the resistance genes into commercial varieties. Marker-assisted selection for these loci should be useful in incorporating SDM resistance genes in maize across environments, even in the absence of the pathogen. Advisors/Committee Members: Magill, Clint W. (advisor), Rooney, William L. (committee member), Frederiksen, Richard A. (committee member), Isakeit, Thomas S. (committee member).

Subjects/Keywords: QTL mapping; Maize; Sorghum downy mildew

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APA (6^th Edition):

Sabry, A. M. (2004). QTL mapping of resistance to sorghum downy mildew in maize. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/460

Chicago Manual of Style (16^th Edition):

Sabry, Ahmed Mohamed-Bashir. “QTL mapping of resistance to sorghum downy mildew in maize.” 2004. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/460.

MLA Handbook (7^th Edition):

Sabry, Ahmed Mohamed-Bashir. “QTL mapping of resistance to sorghum downy mildew in maize.” 2004. Web. 13 Apr 2021.

Vancouver:

Sabry AM. QTL mapping of resistance to sorghum downy mildew in maize. [Internet] [Doctoral dissertation]. Texas A&M University; 2004. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/460.

Council of Science Editors:

Sabry AM. QTL mapping of resistance to sorghum downy mildew in maize. [Doctoral Dissertation]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/460

Texas A&M University

20. Cho, Jae-Min. Isolation and characterization of resistance gene analogs (RGAs) in sorghum.

Degree: PhD, Plant Pathology, 2005, Texas A&M University

URL: http://hdl.handle.net/1969.1/2375

► The largest group of plant disease resistance (R) genes that share similar structures contains a predicted nucleotide-binding site (NBS) domain. NBS domains of this class… (more)

▼ The largest group of plant disease resistance (R) genes that share similar structures contains a predicted nucleotide-binding site (NBS) domain. NBS domains of this class of R genes show highly conserved amino acid motifs, which makes it possible to isolate resistance gene analogs (RGAs) by PCR with degenerate primers and homology searches from public databases. Multiple combinations of degenerate primers were designed from three conserved motifs (one motif was used for a subgroup-specific primer design) in the NBS regions of R genes of various plants. All combinations of primer pairs were used to amplify genomic DNA from sorghum. TIR-specific primer combinations showed no PCR amplification in sorghum. Homology searches identified many NBS-encoding sequences among the expressed or genomic molecular database entries for sorghum. Motif analysis of the sorghum NBS sequences that were identified in this study revealed eight major conserved motifs plus two additional highly conserved motifs, but no TIR-specific motifs. Phylogenetic analysis of sorghum NBS sequences showed tree topology typical of NBS-LRR genes, including clustered nodes and longbranch lengths. Eleven distinct families of NBS sequences, representing a highly diverse sample, were isolated from Sorghum bicolor. With two exceptions, sorghum RGA families appeared to be closely related in sequence to at least one R-gene cloned from other species. In addition, deduced amino acid sequences of sorghum RGAs showed strong sequence similarity to almost all known non-TIR (Toll/Interleukin 1 Receptor)- type R-genes. Mapping with sorghum RGA markers revealed one linkage group containing four out of ten randomly selected markers, suggesting non-random distribution of NBS sequences in the sorghum genome. Rice sequences homologous to sorghum NBS sequences were found from two-way BLAST searches. Some of them were shown to be orthologs, when determined by using phylogenetic approaches which combined five different evolution models and tree-building methods. Advisors/Committee Members: Magill, Clint W. (advisor), Ebbole, Daniel J. (committee member), Pepper, Alan E. (committee member), Stipanovic, Robert D. (committee member).

Subjects/Keywords: NBS-LRR genes; disease resistance; resistance gene analogs; sorghum

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APA (6^th Edition):

Cho, J. (2005). Isolation and characterization of resistance gene analogs (RGAs) in sorghum. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2375

Chicago Manual of Style (16^th Edition):

Cho, Jae-Min. “Isolation and characterization of resistance gene analogs (RGAs) in sorghum.” 2005. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/2375.

MLA Handbook (7^th Edition):

Cho, Jae-Min. “Isolation and characterization of resistance gene analogs (RGAs) in sorghum.” 2005. Web. 13 Apr 2021.

Vancouver:

Cho J. Isolation and characterization of resistance gene analogs (RGAs) in sorghum. [Internet] [Doctoral dissertation]. Texas A&M University; 2005. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/2375.

Council of Science Editors:

Cho J. Isolation and characterization of resistance gene analogs (RGAs) in sorghum. [Doctoral Dissertation]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2375

Texas A&M University

21. Rerngsamran, Panan. Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa.

Degree: PhD, Plant Pathology, 2005, Texas A&M University

URL: http://hdl.handle.net/1969.1/2452

► The fluffy gene of Neurospora crassa is required for asexual sporulation. It encodes an 88 kDa polypeptide containing a typical fungal Zn2Cys6 DNA binding motif.… (more)

▼ The fluffy gene of Neurospora crassa is required for asexual sporulation. It encodes an 88 kDa polypeptide containing a typical fungal Zn2Cys6 DNA binding motif. To identify the target genes on which FL may act, I sought to identify target sequences to which the FL protein binds. Several strategies were attempted to obtain purified FL protein. Purification was achieved by expressing the DNA binding domain of FL in Escherichia coli as a fusion with glutathione S-transferase followed by affinity purification using glutathione sepharose chromatography. DNA binding sites were selected by in vitro binding assays. Comparison of the sequences of selected clones suggested that FL binds to the motif 5??-CGG(N)9CCG-3??. A potential binding site was found in the promoter region of the eas (ccg-2) gene, which encodes a fungal hydrophobin. In vitro competitive binding assays revealed a preferred binding site for FL in the eas promoter, 5??-CGGAAGTTTCCTCCG-3??, which is located 1498 bp upstream of the eas translation initiation codon. In vivo experiments using a foreign DNA sequence tag confirmed that this sequence is a target site for FL regulation. Using Saccharomyces cerevisiae as an experimental system, I demonstrated that the C-terminal portion of FL functions in transcriptional activation. Microarray analysis was performed to study the role of fl in gene regulation on a large scale. mRNA levels in a fl mutant were compared to those in a strain overexpressing the fl gene. Experiments with cDNA microarray containing 13% of the total number of predicted N. crassa genes revealed 122 genes differentially expressed in response to overexpression of fl. Among these, eas displayed the greatest level of response. The cDNA microarray approach also revealed a number of genes that may be indirectly regulated by fl but may be involved in development. This information provides a foundation for further analysis of the role of fl in conidial development. Advisors/Committee Members: Ebbole, Daniel J. (advisor), Magill, Clint W. (committee member), Starr, James L. (committee member), Golden, Susan S. (committee member).

Subjects/Keywords: fungi; transcription factor; Neurospora; conidiation; sporulation

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APA (6^th Edition):

Rerngsamran, P. (2005). Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2452

Chicago Manual of Style (16^th Edition):

Rerngsamran, Panan. “Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa.” 2005. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/2452.

MLA Handbook (7^th Edition):

Rerngsamran, Panan. “Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa.” 2005. Web. 13 Apr 2021.

Vancouver:

Rerngsamran P. Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa. [Internet] [Doctoral dissertation]. Texas A&M University; 2005. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/2452.

Council of Science Editors:

Rerngsamran P. Functional analysis of fluffy, a transcriptional regulator for conidial development in Neurospora crassa. [Doctoral Dissertation]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2452

Texas A&M University

22. Jiang, Yiming. Rice Transformation as a Means to Study Gene Expression.

Degree: PhD, Genetics, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-750

► An exceptionally effective transformation procedure has been established by using class I embryo-derived rice callus. Every treated callus clump yielded multiple independently transformed plants (average… (more)

▼ An exceptionally effective transformation procedure has been established by using class I embryo-derived rice callus. Every treated callus clump yielded multiple independently transformed plants (average 40 plantlets). Analysis of genomic DNA blots and pollen expressing green fluorescent protein (GFP) from T0 plants revealed that 64% bore a single locus T-DNA insertion in which half had one T-DNA copy. Additive transgene expression was observed fromT0 plants with GFP driven by mUbi1 promoter. Transgenic plants could be rapidly characterized by analyzing GFP pollen from T0 plants without the need for further generations or genomic DNA blot analysis. Agrobacterium tumefaciens-mediated transformation of microspore-derived callus for generating large numbers of T-DNA haploid and doubled haploid(DH) plants has also been investigated. The established transformation procedure resulted in 100% transformation frequency for class I microspore-derived rice callus. Each callus typically yields multiple independent transgenic plants. Genomic DNA blot analysis suggested 98% of the transgenic plants are independent events. About half of the transgenic plants were identified as haploid plants, whereas half are DH hemizygous or homozygous transgenic plants. DH homozygous transgenic plants were obtained from T0plants and confirmed by pollen GFP expression and genomic blot analysis in T0transgenic DH plants. In this study, about 60% ofT0transgenic DH plants had a single locus T-DNA insertion of which 45% bore one T-DNA copy. Furthermore, in a population of over 2,000 haploid and doubled haploid T-DNA plants , about 25% showed phenotypic differences from non-transformed haploid plants. Approximately 5% were seriously phenotypically abnormal including lethal or semi-lethal mutants. This highly efficient transformation procedure using microspore-derived callus could be valuable in speeding up plant breeding and in new gene discovery. Diversification of the mUbi1 promoter led to a minimal promoter that has a similar function as the original mUbi1. Transient and stable transformation as measured from gene expression driven by the minimal promoter suggested that it has a similar function as the original wild type promoter. Advisors/Committee Members: Hall, Timothy C. (advisor), Magill, Clint (committee member), Gould, Jean (committee member), Rathore, Keerti S. (committee member).

Subjects/Keywords: Transformation; Diploid; Haploid; Doubled Haploid; maize Ubi1 promoter

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APA (6^th Edition):

Jiang, Y. (2010). Rice Transformation as a Means to Study Gene Expression. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-750

Chicago Manual of Style (16^th Edition):

Jiang, Yiming. “Rice Transformation as a Means to Study Gene Expression.” 2010. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-750.

MLA Handbook (7^th Edition):

Jiang, Yiming. “Rice Transformation as a Means to Study Gene Expression.” 2010. Web. 13 Apr 2021.

Vancouver:

Jiang Y. Rice Transformation as a Means to Study Gene Expression. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-750.

Council of Science Editors:

Jiang Y. Rice Transformation as a Means to Study Gene Expression. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-750

Texas A&M University

23. Spencer, Angela M. Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island.

Degree: MS, Veterinary Parasitology, 2006, Texas A&M University

URL: http://hdl.handle.net/1969.1/4175

► A Babesia sp. isolated from eastern cottontail rabbits (Sylvilagus floridanus) is morphologically similar and genetically identical, based on SSU rRNA gene comparisons, to two agents… (more)

Subjects/Keywords: Babesia; Babesia divergens; zoonotic Babesia

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APA (6^th Edition):

Spencer, A. M. (2006). Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4175

Chicago Manual of Style (16^th Edition):

Spencer, Angela M. “Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island.” 2006. Masters Thesis, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/4175.

MLA Handbook (7^th Edition):

Spencer, Angela M. “Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island.” 2006. Web. 13 Apr 2021.

Vancouver:

Spencer AM. Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island. [Internet] [Masters thesis]. Texas A&M University; 2006. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/4175.

Council of Science Editors:

Spencer AM. Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island. [Masters Thesis]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/4175

Texas A&M University

24. Shi, Xiangyu. STUDY OF GENE SILENCING IN RICE: A ROOT PREFERENTIAL GENE RCG2.

Degree: PhD, Genetics, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-460

► The RCg2 promoter was identified in a search for root-specific genes to combat the rice water weevil (RWW) but expressed at low frequency (~10%). Spatial… (more)

▼ The RCg2 promoter was identified in a search for root-specific genes to combat the rice water weevil (RWW) but expressed at low frequency (~10%). Spatial expression of RCg2 was investigated using two reporter constructs YXA (RCg2-gus-ocs) and YXB (RCg2-gus-RCg2) that included 1.6 kb of the RCg2 5' sequence fused to the ?-glucuronidase (gus) coding region. YXB plants were generated via Agrobacterium-mediated transformation but only 8 of 158 plants analyzed showed strong GUS activity despite the presence of an intact construct. Reactivation of RCg2 gene in rice was investigated by treatment of R0 and R1 of YXB transgenic plants with 5-azacytidine. Reactivation of RCg2-gus was observed in some transgenic plants indicating different mechanisms involved in the gene silencing of the YXB lines. DNA methylation analysis, northern blotting, RT-PCR and small RNA analysis supported the conclusion that PTGS and TGS are present in the silenced plants. Promoter analysis in silico and using promoter deletion assays predicted that the RCg2 promoter contains a complex region that includes miRNA homologs, MITEs and repetitive sequences. The high frequency of promoter-related silencing suggests functional interactions of these elements of the transgene and the homologous endogenous gene. To identify key elements contributing to the root-preferential expression of RCg2 and the high frequency of silencing observed in transgenic (YXB) lines, several RCg2 promoter deletion constructs were designed. These include 5' deletions MC1, MC2, MC4, MC7 and MC8 and internal deletions MC5, MC11, MC12 and MC13. The frequency with which silencing was encountered in populations of the deletion mutants was used to characterize the effects of various promoter elements. Deletion of the region from -406 to -208 (compared MC11 to YXB, and MC13 to MC1) revealed that region contains a negative element. Among 36 independent transformants, 33% with MC11 expressed GUS and 85% with MC13 showed GUS expression. Comparing MC7 transgenic plants to MC1 revealed that the region ?888 to ?729 is another negative regulatory element, and comparing MC11 to MC12, the proportion of expression of transgenic plants indicated the region ?729 to ?406 is a positive regulatory element. Advisors/Committee Members: Hall, Timothy C. (advisor), Magill, Clint W. (committee member), Gould, Jean H. (committee member), Chen, Z. Jeffrey (committee member).

Subjects/Keywords: Rice (Oryza sativa L.); DNA methylation sequence; gene silencing; Agrobacterium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shi, X. (2010). STUDY OF GENE SILENCING IN RICE: A ROOT PREFERENTIAL GENE RCG2. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-460

Chicago Manual of Style (16^th Edition):

Shi, Xiangyu. “STUDY OF GENE SILENCING IN RICE: A ROOT PREFERENTIAL GENE RCG2.” 2010. Doctoral Dissertation, Texas A&M University. Accessed April 13, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-460.

MLA Handbook (7^th Edition):

Shi, Xiangyu. “STUDY OF GENE SILENCING IN RICE: A ROOT PREFERENTIAL GENE RCG2.” 2010. Web. 13 Apr 2021.

Vancouver:

Shi X. STUDY OF GENE SILENCING IN RICE: A ROOT PREFERENTIAL GENE RCG2. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-460.

Council of Science Editors:

Shi X. STUDY OF GENE SILENCING IN RICE: A ROOT PREFERENTIAL GENE RCG2. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-05-460

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