+publisher:"Texas A&M University" +contributor:("Holman, Patricia J.")

Texas A&M University

1. Fisher, Amanda. Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer.

Degree: MS, Laboratory Animal Medicine, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676

► Babesia bovis has been an important disease agent in the U.S. cattle industry for over a century. Recently, B. bovis-like parasites have been identified in… (more)

▼ Babesia bovis has been an important disease agent in the U.S. cattle industry for over a century. Recently, B. bovis-like parasites have been identified in white-tailed deer (WTD; Odocoileus virginianus) in Texas. If the parasites found in the WTD are B. bovis that are able to infect cattle, the disease could re-emerge. Susceptible adult cattle often die from this disease, which would result in severe production losses, as well as a decrease in carcass weights of disease survivors. The B. bovis-like parasite found in WTD was compared to B. bovis from cattle, by ribosomal DNA sequence analysis. Babesia isolated from WTD were found to have 99% identity to B. bovis from GenBank cattle sequences. No cattle samples in this study were found to be positive for B. bovis. On culture of WTD samples, a Babesia parasite could not be visualized based on common morphological features. Trypanosoma cervi has been studied for decades, but all the previous research identified this parasite solely by morphology. Trypanosoma species obtained from different host species was compared by ribosomal DNA sequence analyses. In this study, the Trypanosoma cultured from WTD had the morphological appearance of T. cervi. On sequence analysis, the cattle sequences aligned together with cattle isolates and the WTD sequences aligned closely with elk (Cervus canadensis) sequences, indicating that wild ungulates (WTD and elk) and cattle most likely have separate trypanosome species. On distribution analysis there was a trend in three South Texas counties, where the county with the highest occurrence of Trypanosoma had the lowest occurrence of Babesia; and vice versa. It is possible that Trypanosoma and Babesia blood parasites compete within the mammalian host, but the chi-squared test did not show a significant association between the two parasites in the different counties. On seasonal analysis, the correlation between positive samples and season could not be statistically confirmed, but it appears that Babesia infected animals are found in lowest numbers during hot, dry seasons. It also appears that there is another vector for Trypanosoma in South Texas besides the ked (Lipoptena mazamae) and tabanid fly (Tabanus spp.). Advisors/Committee Members: Davis, Donald (advisor), Holman, Patricia J. (advisor), Gresham, Vincent C. (committee member).

Subjects/Keywords: parasite; Trypanosome; Trypanosoma theileri, Trypanosoma cervi; Babesia; cattle; white-tailed deer

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APA (6^th Edition):

Fisher, A. (2012). Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676

Chicago Manual of Style (16^th Edition):

Fisher, Amanda. “Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer.” 2012. Masters Thesis, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676.

MLA Handbook (7^th Edition):

Fisher, Amanda. “Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer.” 2012. Web. 08 Mar 2021.

Vancouver:

Fisher A. Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676.

Council of Science Editors:

Fisher A. Morphological and Genetic Comparisons between Babesia bovis and Trypanosoma spp. Found in Cattle and White-tailed Deer. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11676

Texas A&M University

2. Lin, Huaiying 1986-. Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle.

Degree: MS, Biomedical Sciences, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/149237

► Babesia bovis is a causative agent of bovine babesiosis and is transmitted by vector ticks, Rhipicephalus (Boophilus) spp. The disease has a high mortality rate… (more)

▼ Babesia bovis is a causative agent of bovine babesiosis and is transmitted by vector ticks, Rhipicephalus (Boophilus) spp. The disease has a high mortality rate in susceptible cattle, causing serious economic loss. At present, the only commercial vaccine is culture-based with limited availability. No effective molecular vaccine has been developed to date. Generating a vaccine with specific critical epitopes responsible for protection against B. bovis is critically important. Immunity against B. bovis requires both innate and adaptive responses, with antigen-specific CD4+ T cells essential to the latter through production of IFN-γ. Fourteen B. bovis proteins were selected as putative vaccine candidates and their full-length genes cloned for recombinant protein production intended for evaluating peripheral blood mononuclear cell IFN-γ secretion level from experimentally infected animals in ELISPOT. All proteins expressed in insoluble form (inclusion bodies) and could not be purified. B. bovis genes were then truncated to exclude signal peptide and transmembrane regions, then cloned and expressed using pET101/D-TOPO in Escherichia coli to obtain soluble, useable proteins. Only recombinant B. bovis MSA1, MSA2b and MSA2a1 proteins were successfully expressed in soluble form. These proteins induce invasion-blocking antibodies in immunized cattle, are hypothesized to elicit protection in susceptible animals, but were previously studied by others. Due to failure to produce new candidates to assay, the animal experiments were not performed. Instead, sera from field-infected cattle were assayed for reactivity against the MSA proteins by indirect immunofluorescent antibody (IFA) and western blot (WB) analysis. Field sera from South Texas (#41) and the Mexican Yucatan (#6,9 and #11) along with positive and negative controls were tested. In IFA test, cattle #6, #9 and #41 were positive while #11 was negative. In WB, #41 and #6 reacted with the recombinant MSA proteins and with control B. bovis whole parasite lysate. However, both #11 and #9 had no signal in WB, although the latter was positive in IFA. Several theories may explain this phenomenon, such as the different preparation process of the antigen in the two tests, strain differences between sera and test antigens, or the different design and nature of each test. Advisors/Committee Members: Holman, Patricia J (advisor), Mwangi, Waithaka (committee member), Teel, Pete D (committee member), Freeman, Jeanne (committee member).

Subjects/Keywords: Babesia bovis; MSA; protein expression in E. coli; Western Blot

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APA (6^th Edition):

Lin, H. 1. (2013). Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/149237

Chicago Manual of Style (16^th Edition):

Lin, Huaiying 1986-. “Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle.” 2013. Masters Thesis, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/149237.

MLA Handbook (7^th Edition):

Lin, Huaiying 1986-. “Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle.” 2013. Web. 08 Mar 2021.

Vancouver:

Lin H1. Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/149237.

Council of Science Editors:

Lin H1. Expression and Evaluation of Recombinant Babesia bovis Antigens of Vaccine Potential Against Tick Fever in Cattle. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/149237

Texas A&M University

3. Alhaboubi, Amer Rasoolfadhl. In Vitro Growth and Molecular Characterization of Vector-Borne Intraerythrocytic Parasites of Domestic Animals and Wildlife.

Degree: PhD, Veterinary Pathobiology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173687

► Haemogregarines are a group of blood sporozoans that parasitize reptiles, most commonly turtles, or tortoises. Haemogregarine-like inclusions in the red blood cells of a severely… (more)

▼ Haemogregarines are a group of blood sporozoans that parasitize reptiles, most commonly turtles, or tortoises. Haemogregarine-like inclusions in the red blood cells of a severely underweight alligator snapping turtle Macrochelys temminckii Troost in Harlan were examined in this study. The morphology and morphometric data for intraerythrocytic forms found on microscopic examination were similar to Haemogregarina macrochelysi n. sp. previously reported in the same species. The 18S ribosomal RNA (18S rRNA) gene was cloned and five sequences deposited in the NCBI GenBank® database. All five showed ∼96 % identity to Haemogregarina balli, Hepatozoon sp., and Hemolivia stellata. A phylogenetic tree generated from the five sequences aligned with 18S rDNA sequences of other hematozoa and two outgroup species revealed the cloned sequences clustered on their own branch within the Haemogregarina spp. clade. There is no genetic data for H. macrochelysi n. sp., so it is unclear if the Texas turtle parasite is conspecific with H. macrochelysi n. sp. Babesia spp. are intraerythrocytic protozoans that parasitize mammals. Cultured Babesia bovis and Babesia bigemina, parasites of cattle, were recovered from liquid nitrogen (LN₂) storage nearly 30 years after cryopreservation. Four cattle were compared as donors of red blood cells (RBC) and serum for microaerophilous stationary phase (MASP) cultures in the recovery of B. bigemina. RBC and serum from only one donor supported the growth of B. bigemina. Two B. bigemina (frozen in 1986 and 1987) and two B. bovis (both frozen in 1986) cryostocks were resuscitated from LN₂ storage and all four recovered and thrived in the donor bovine RBC and serum. In the 3rd passage after recovery, B. bovis cultures were cryopreserved. Six months later they were successfully recovered from LN₂ using RBC and serum from the same donor. This study shows that B. bovis and B. bigemina stored nearly 30 years in LN₂ can be successfully recovered in the MASP system. This study also confirms previous observations that selection of a suitable bovine donor of RBC and serum is critical to the success of the Babesia sp. culture. Two markers, 18S rRNA gene and rRNA intervening transcribed spacer regions 1 and 2 (ITS), in B. bovis and B. bigemina from Puerto Rico (PR) cattle and archived culture samples from Mexico (B. bovis) and the Virgin Islands (B. bigemina) were PCR amplified, cloned and sequenced. In total, 54 18S rDNA and 21 ITS sequences were deposited in GenBank. The identity scores among the PR B. bovis 18S rDNA cloned sequences were 92.3% to 100%, and 97.7% to 99.99% among the archived Mexico B. bovis. PR and the Virgin Islands B. bigemina 18S rDNA sequence identity scores ranged from 99.1% to 99.98%. The UPMGA cladogram generated from 18S rDNA sequences shows the clear distinction of B. bovis and B. bigemina (and B. ovis). The PR ITS cloned sequences showed 69.3% to 100% identity among them. In the UPMGA cladogram, the PR sequences fell into seven different groups, except for one outlier that branched… Advisors/Committee Members: Esteve-Gasent, Maria D (advisor), Holman, Patricia J (advisor), Logan, Linda (committee member), Pérez de Léon, Adalberto A (committee member), Berghman, Luc R (committee member).

Subjects/Keywords: Blood sporozoans; Haemogregarines; Babesia bovis; Babesia bigemina; culture; Genotying

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APA (6^th Edition):

Alhaboubi, A. R. (2018). In Vitro Growth and Molecular Characterization of Vector-Borne Intraerythrocytic Parasites of Domestic Animals and Wildlife. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173687

Chicago Manual of Style (16^th Edition):

Alhaboubi, Amer Rasoolfadhl. “In Vitro Growth and Molecular Characterization of Vector-Borne Intraerythrocytic Parasites of Domestic Animals and Wildlife.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/173687.

MLA Handbook (7^th Edition):

Alhaboubi, Amer Rasoolfadhl. “In Vitro Growth and Molecular Characterization of Vector-Borne Intraerythrocytic Parasites of Domestic Animals and Wildlife.” 2018. Web. 08 Mar 2021.

Vancouver:

Alhaboubi AR. In Vitro Growth and Molecular Characterization of Vector-Borne Intraerythrocytic Parasites of Domestic Animals and Wildlife. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/173687.

Council of Science Editors:

Alhaboubi AR. In Vitro Growth and Molecular Characterization of Vector-Borne Intraerythrocytic Parasites of Domestic Animals and Wildlife. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173687

4. Baradji, Issa. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.

Degree: PhD, Veterinary Microbiology, 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

► Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected… (more)

▼ Babesia microti is a tickborne hemoprotozoan parasite that causes the disease babesiosis in humans. Babesia microti Apical Membrane Antigen-1 (AMA-1) is a micronemal protein suspected to play a role in erythrocyte invasion. To investigate interaction between AMA-1 and the host cell, the ectodomain region of the B. microti ama-1 gene was cloned into an expression vector, expressed as a histidine-tagged fusion protein, and used to probe red blood cell membrane proteins in far Western blot assays. The B. microti ama-1 ectodomain, which excludes the signal peptide and the transmembrane region of the open reading frame, was amplified from a cloned gene sequence. The AMA-1 ectodomain is a membrane bound polypeptide that extends into the extracellular space and is most likely to interact or initiate interaction with the host red blood cell surface receptor(s). The amplicon was ligated into a protein expression vector to produce a 58.1 kDa recombinant His-tagged fusion protein, which was confirmed by Western blot analysis. The recombinant B. microti AMA-1 fusion protein was enriched on nickel affinity columns and then used to probe mouse, human and horse red blood cell membrane proteins in far Western blot assays. Babesia microti AMA-1 consistently reacted strongly with a protein migrating at 49 kDa. A similar reaction occurred between the B. microti AMA-1 and horse red blood cell membrane proteins, suggesting that similar interacting proteins of this size are shared by red blood cells from the three species. The B. microti AMA-1 may bind to red blood cell membrane sialic-acid groups, as shown for other Babesia spp. This may explain the signal at the 49 kDa position observed between B. microti AMA-1 and red blood cell membrane proteins from three different species. Further studies may determine if the binding epitopes of the red blood cell binding partner at this position vary and contribute to the specificity of each parasite AMA-1 for their respective host cells. Advisors/Committee Members: Holman, Patricia J. (advisor), Ball, Judith M. (committee member), Magill, Clint C. (committee member), Womack, James E. (committee member).

Subjects/Keywords: Babesia microti; Apical Membrane Antigen-1; AMA-1; erythrocyte; parasite ligand; red blood cell; receptor; protein-protein interaction

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APA (6^th Edition):

Baradji, I. (2010). The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

Chicago Manual of Style (16^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Doctoral Dissertation, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

MLA Handbook (7^th Edition):

Baradji, Issa. “The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti.” 2010. Web. 08 Mar 2021.

Vancouver:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Internet] [Doctoral dissertation]. Texas A&M University; 2010. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68.

Council of Science Editors:

Baradji I. The Role of Apical Membrane Antigen-1 in Erythrocyte Invasion by the Zoonotic Apicomplexan Babesia microti. [Doctoral Dissertation]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-68

5. Brannan, Jaime Lynette. Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks.

Degree: PhD, Genetics, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151106

► Infestation of cattle by Lone Star ticks, Amblyomma americanum, leads to damage of hides intended for leather, weight loss, infertility, and potentially death of cattle,… (more)

▼ Infestation of cattle by Lone Star ticks, Amblyomma americanum, leads to damage of hides intended for leather, weight loss, infertility, and potentially death of cattle, which contribute to production losses for farmers. Public concerns regarding chemical residues in food and the environment necessitate development of chemical-free alternative tick controls, such as breeding for tick-resistant phenotypes and developing anti-tick vaccines. Thus, the goal of this study was elucidation of mechanisms that mediate immune responses in cattle infested with A. americanum using gene expression techniques. Methods for isolation of total RNA from bovine tick bite-site biopsies and blood leukocytes were optimized to provide RNA suitable for gene expression studies. Tick bite-site biopsies (6 mm) and blood leukocytes were collected from a total of 13 calves (N=6, Group 4 and N=7, Group 5 calves) during experimental tick infestations to determine A. americanum tick-susceptible and -resistant phenotypes. Microarray experiments compared gene expression in tick bite-sites from tick-susceptible, moderately tick-resistant, and highly tick-resistant calves. A total of 35 genes were profiled in tick bite-site biopsies and 12 genes were evaluated in blood leukocytes via gene-specific qRT-PCR assays, and analyzed for each phenotype and for each group of calves as a whole. Analysis of microarray data revealed differential expression of IL-1R-mediators among the three cattle phenotypes. Expression profiles generated by qRT-PCR for TLR-mediating genes such as TLR2, TLR4, CD14, and MyD88 suggest that a MyD88-dependent signaling pathway may mediate the development of acquired immunity in cattle infested with Lone Star ticks. Additionally, increased expression for IL12, IFNgamma, and TNFalpha suggests that a Th1-type cell-mediated reaction may be activated, whereas increased expression of IL6, IL10, and IGHG1 supports the involvement of a Th2-type humoral-mediated response at tick bite-sites in cattle infested with at A. americanum. Regression analyses identified strong correlations between factors involved in pattern recognition in tick bite-site biopsies, including associations between TLR4 and IL1alpha, and between IL1alpha and IL1RN. In conclusion, this dissertation reports optimal methodology for gene expression studies in tick-infested cattle and provides preliminary data concerning the underlying mechanisms associated with the immune response in Lone Star tick-infested cattle. Advisors/Committee Members: Holman, Patricia J (advisor), Womack, James E (advisor), Riggs, Penny K (committee member), Tizard, Ian R (committee member), Pruett, John H (committee member).

Subjects/Keywords: gene expression; qRT-PCR; Amblyomma americanum; tick-resistance; cattle

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APA (6^th Edition):

Brannan, J. L. (2013). Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151106

Chicago Manual of Style (16^th Edition):

Brannan, Jaime Lynette. “Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks.” 2013. Doctoral Dissertation, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/151106.

MLA Handbook (7^th Edition):

Brannan, Jaime Lynette. “Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks.” 2013. Web. 08 Mar 2021.

Vancouver:

Brannan JL. Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/151106.

Council of Science Editors:

Brannan JL. Transcriptional Profiling of Immune Responses in Cattle Experimentally Infested with Amblyomma americanum ticks. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151106

Texas A&M University

6. Waters, Paulette Francesca. Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts.

Degree: MS, Veterinary Microbiology, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/330

► Molecular and in vitro comparisons were performed using two isolates of Encephalitozoon hellem, one from an avian host and one from a human host, and… (more)

Subjects/Keywords: microsporidia; Encephalitozoon; zoonoses; birds; in vitro; parasite

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APA (6^th Edition):

Waters, P. F. (2004). Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/330

Chicago Manual of Style (16^th Edition):

Waters, Paulette Francesca. “Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts.” 2004. Masters Thesis, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/330.

MLA Handbook (7^th Edition):

Waters, Paulette Francesca. “Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts.” 2004. Web. 08 Mar 2021.

Vancouver:

Waters PF. Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts. [Internet] [Masters thesis]. Texas A&M University; 2004. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/330.

Council of Science Editors:

Waters PF. Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts. [Masters Thesis]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/330

Texas A&M University

7. Schoelkopf, Lorien. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.

Degree: MS, Veterinary Parasitology, 2005, Texas A&M University

URL: http://hdl.handle.net/1969.1/2660

► Babesia odocoilei is an intraerythrocytic apicomplexan parasite which infects cervidae, sometimes causing babesiosis. It is vectored by the tick Ixodes scapularis and is distributed throughout… (more)

▼ Babesia odocoilei is an intraerythrocytic apicomplexan parasite which infects cervidae, sometimes causing babesiosis. It is vectored by the tick Ixodes scapularis and is distributed throughout the southeastern United States. The geographic and host range continue to extend as new incidence of infection is detected. A genomic DNA region spanning the internal transcribed spacer 1 (ITS1), 5.8S rRNA gene, and ITS2 of ribosomal RNA (rRNA) from 18 B. odocoilei isolates (speciation confirmed by small subunit rRNA analysis) was amplified using the polymerase chain reaction, cloned and sequenced. The isolates originated from 6 different cervidae or bovidae hosts in various U.S. geographic areas. Included in the analysis was a previously described reindeer B. odocoilei-like isolate, RD61, which showed only 99.0% identity in SSU rRNA analysis to B. odocoilei. Percent identity pairwise comparisons among the samples were calculated for both the full ITS1-5.8S-ITS2 and individual genomic regions. Identity values for all comparisons ranged from 90% to 100%, with the exception of RD61, which showed no higher than 88% identity for all gene regions. An analysis of fixed differences identified in the ITS1 and ITS2 gene regions of all clones revealed 21 fixed differences in ITS1, and only 11 in ITS2. Most isolates were found to have 2 overall patterns of fixed differences, although some had 1 or 3. Phylogenetic analysis of all sequences for the entire ITS1-5.8S-ITS2 gene region placed most isolates into 2 distinct groups corresponding to those observed in the analysis of fixed differences. This suggested the presence of at least 2 rRNA transcription units in B. odocoilei. ITS analysis failed to demonstrate host or geographic differences that might serve to pinpoint the source of outbreaks of B. odocoilei in farmed and managed host animals. This failure might result from genetic recombination of ITS genomic regions during the tick vector stage. Lack of conspecificity between the RD61 isolate and B. odocoilei was supported by this study; however, more data are needed to clarify the taxonomic status of this B. odocoilei-like isolate. Advisors/Committee Members: Holman, Patricia J. (advisor), Teel, Pete D. (committee member), Craig, Thomas M. (committee member).

Subjects/Keywords: Babesia odocoilei; Internal transcribed spacers (ITS); Ribosomal RNA

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APA (6^th Edition):

Schoelkopf, L. (2005). Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2660

Chicago Manual of Style (16^th Edition):

Schoelkopf, Lorien. “Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.” 2005. Masters Thesis, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/2660.

MLA Handbook (7^th Edition):

Schoelkopf, Lorien. “Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.” 2005. Web. 08 Mar 2021.

Vancouver:

Schoelkopf L. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. [Internet] [Masters thesis]. Texas A&M University; 2005. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/2660.

Council of Science Editors:

Schoelkopf L. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. [Masters Thesis]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2660

Texas A&M University

8. Spencer, Angela M. Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island.

Degree: MS, Veterinary Parasitology, 2006, Texas A&M University

URL: http://hdl.handle.net/1969.1/4175

► A Babesia sp. isolated from eastern cottontail rabbits (Sylvilagus floridanus) is morphologically similar and genetically identical, based on SSU rRNA gene comparisons, to two agents… (more)

Subjects/Keywords: Babesia; Babesia divergens; zoonotic Babesia

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APA (6^th Edition):

Spencer, A. M. (2006). Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4175

Chicago Manual of Style (16^th Edition):

Spencer, Angela M. “Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island.” 2006. Masters Thesis, Texas A&M University. Accessed March 08, 2021. http://hdl.handle.net/1969.1/4175.

MLA Handbook (7^th Edition):

Spencer, Angela M. “Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island.” 2006. Web. 08 Mar 2021.

Vancouver:

Spencer AM. Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island. [Internet] [Masters thesis]. Texas A&M University; 2006. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1969.1/4175.

Council of Science Editors:

Spencer AM. Molecular and in vitro characterization of a Babesia divergens-like agent from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island. [Masters Thesis]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/4175

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