+publisher:"Texas A&M University" +contributor:("Criscitiello, Michael")

Texas A&M University

1. Seelye, Stacie Lynn. Genomic Organization of Zebrafish (Danio rerio) T Cell Receptor α/δ Locus and Analysis of Expressed Products.

Degree: MS, Biomedical Sciences, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/156980

► In testing the hypothesis that all jawed vertebrate classes employ immunoglobulin heavy chain V (IgHV) gene segments in their T cell receptor (TCR)δ encoding loci,… (more)

Subjects/Keywords: T Cell Receptor α/δ; Danio rerio

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Seelye, S. L. (2016). Genomic Organization of Zebrafish (Danio rerio) T Cell Receptor α/δ Locus and Analysis of Expressed Products. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156980

Chicago Manual of Style (16^th Edition):

Seelye, Stacie Lynn. “Genomic Organization of Zebrafish (Danio rerio) T Cell Receptor α/δ Locus and Analysis of Expressed Products.” 2016. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/156980.

MLA Handbook (7^th Edition):

Seelye, Stacie Lynn. “Genomic Organization of Zebrafish (Danio rerio) T Cell Receptor α/δ Locus and Analysis of Expressed Products.” 2016. Web. 03 Mar 2021.

Vancouver:

Seelye SL. Genomic Organization of Zebrafish (Danio rerio) T Cell Receptor α/δ Locus and Analysis of Expressed Products. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/156980.

Council of Science Editors:

Seelye SL. Genomic Organization of Zebrafish (Danio rerio) T Cell Receptor α/δ Locus and Analysis of Expressed Products. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/156980

Texas A&M University

2. Vuong, Christine. Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine.

Degree: MS, Biomedical Sciences, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11706

► Although avian influenza virus (AIV) infections in domestic poultry are uncommon, transmission of avian influenza from wild waterfowl reservoirs does occur. Depopulation of the infected… (more)

▼ Although avian influenza virus (AIV) infections in domestic poultry are uncommon, transmission of avian influenza from wild waterfowl reservoirs does occur. Depopulation of the infected flock is the typical response to AIV outbreaks in domestic chicken production, causing a loss in profits and accumulation of unexpected expenses. Because it is impossible to know which of many virus subtypes will cause an outbreak, it is not feasible for the U.S. to stockpile vaccines against all possible avian influenza threats. Currently, the U.S. does not routinely vaccinate chickens against influenza due to the inability to differentiate infected from vaccinated animals (DIVA), which would place limitations on its trade markets. A Sindbis virus vector expressing the PR8 influenza strain's M2e peptide was developed as a potential universal DIVA vaccine. M2e is a conserved peptide amongst influenza A viruses; M2e-specific antibodies induce antibody-dependent cytotoxicity or phagocytosis of infected cells, reducing production and shedding of AIV during infection. In this study, chickens were vaccinated at one-month-of-age with parental (E2S1) or recombinant Sindbis viruses expressing the PR8 M2e peptide (E2S1-M2e) by subcutaneous or intranasal routes at high (106 pfu) or low (103 pfu) dosages. Chickens were boosted at 2-weeks post-initial vaccination using the same virus, route, and dosage, then challenged with low pathogenic H5N3 AIV at 0.2 mL of 106/mL EID50 2-weeks post-boost. Serum samples were collected at 1-week and 2-weeks post-vaccination, 2-weeks post-boost, and 2-weeks post-challenge and screened for PR8 M2e-specific IgY antibody production by ELISA. Both high and low dose subcutaneously, as well as high dose intranasally vaccinated E2S1-M2e groups produced significantly higher levels of PR8 M2e-specific IgY antibodies as early as 1-week post-vaccination, while the uninoculated control and E2S1 groups remained negative for all pre-challenge time points. M2e-specific IgY antibodies capable of binding the challenge H5N3 M2e peptide were detected in groups with existing vaccine-induced M2e-specific antibodies pre-challenge, suggesting antibody M2e cross-reactivity. After challenge, all groups developed M2e-specific IgY antibodies and high HI titers, verifying successful AIV infection during challenge and production of hemagglutinin-specific antibodies. Viral shedding titers 4-days post-challenge were used to measure vaccine efficacy and were similar amongst all groups. Microneutralization assay results confirmed that post-boost serum samples, containing only M2e-specific antibodies, were unable to neutralize AIV in vitro. Although the E2S1-M2e vaccine was capable of producing high levels of M2e-specific IgY antibodies when inoculated subcutaneously, these antibodies were not able to reduce viral shedding and therefore did not protect chickens from AIV. Advisors/Committee Members: Lupiani, Blanca (advisor), Reddy, Sanjay (committee member), Criscitiello, Michael (committee member).

Subjects/Keywords: avian influenza; AIV; M2e; sindbis; universal vaccine; DIVA; chicken

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vuong, C. (2012). Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11706

Chicago Manual of Style (16^th Edition):

Vuong, Christine. “Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine.” 2012. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11706.

MLA Handbook (7^th Edition):

Vuong, Christine. “Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine.” 2012. Web. 03 Mar 2021.

Vancouver:

Vuong C. Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11706.

Council of Science Editors:

Vuong C. Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11706

Texas A&M University

3. Georges, Hanah Michale. Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR.

Degree: MS, Biomedical Sciences, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/166011

► Viral epidemics in the pork industry continue to plague the agricultural industry, national food security, and human health. Current preventative measures are insufficient for deterrence… (more)

▼ Viral epidemics in the pork industry continue to plague the agricultural industry, national food security, and human health. Current preventative measures are insufficient for deterrence as viruses rapidly mutate and reemerge despite vaccinations and biosecurity. In order to prevent viral outbreaks within and between pork facilities, alternative measures are needed. The innate immune system is a host’s first response to pathogens. It offers a rapid response; however, it does not have the specificity which is seen in the adaptive immune system. In its initial response, cytokines IFNα and IFNβ induce expression of interferon stimulated genes to inhibit viral replication within the cell. IRF-7 has been identified as the master regulator of type I IFN transcription and is tightly controlled by OASL and 4E-BP1, binding to the secondary structure of its 5’ UTR. To enhance the innate immune system response to viruses, the DNA sequence for the 5’ UTR of IRF-7 in porcine cells was modified using the CRISPR/Cas9 gene editing system. Following UTR modifications, 9 modified cell lines were produced and clonally selected, along with 2 control cell lines that were not modified. All cell lines were transfected with poly I:C to induce the innate immune response and transcript levels of genes associated with the IFN pathway analyzed by RT-qPCR. Deletions induced by the CRISPR/Cas9 system in cell lines successfully altered the IRF-7 5’ UTR DNA sequence in 82% of cells. RNA folding predictions of the two modified cell lines with a heightened immune response show drastic alterations to the IRF-7 5’ UTR in regions hypothesized to be critical for IRF-7 translational regulation. Understanding the relationship of the 5’ UTR secondary structure and IRF-7 regulation allows for the possibility of enhanced innate immune responses in animal models with IRF-7 5’ UTR modifications or even in targeting the IRF-7 5’ UTR in humans using pharmacological substances. Advisors/Committee Members: Long, Charles R (advisor), Golding, Michael (committee member), Criscitiello, Michael (committee member).

Subjects/Keywords: IRF-7; 5' UTR; Porcine; Interferon Regulatory Factor 7

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Georges, H. M. (2017). Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/166011

Chicago Manual of Style (16^th Edition):

Georges, Hanah Michale. “Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR.” 2017. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/166011.

MLA Handbook (7^th Edition):

Georges, Hanah Michale. “Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR.” 2017. Web. 03 Mar 2021.

Vancouver:

Georges HM. Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/166011.

Council of Science Editors:

Georges HM. Enhancing the Innate Immune Response in Porcine Cells Through Genetic Manipulation of the IRF-7 5’ UTR. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/166011

Texas A&M University

4. Rychlik, Kristal Ann. Prenatal Exposure to Particulate Air Pollution and Effects on Postnatal Immune Response.

Degree: PhD, Toxicology, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/161638

► There is considerable evidence showing that exposure to particulate matter air pollution during important developmental windows, such as the prenatal period, can cause adverse respiratory… (more)

▼ There is considerable evidence showing that exposure to particulate matter air pollution during important developmental windows, such as the prenatal period, can cause adverse respiratory outcomes. Mechanisms underlying increased risks from in utero exposure are largely unknown. Since epigenetic modifications have been recognized as an important mediator of developmental reprogramming following environmental exposures in early life, the primary objective of this research was to establish a representative model of prenatal air pollution exposure to probe underlying mechanisms leading to adverse respiratory responses in offspring. The preliminary study (aim 1) established the proof-of-principle for differential air pollution-induced epigenetic changes across varying genetic background. Two strains (BALB/c and C57Bl/6 mice) were exposed to diesel exhaust particulate matter (DEPM), a major constituent of outdoor air pollution, throughout pregnancy. Following sacrifice at postnatal day 2, offspring global DNA methylation and hydroxymethylation was quantified in lung tissue. Results indicate differential methylation in BALB/c mice but not C57Bl/6. In aim 2, BALB/c and C57Bl/6 dams were exposed to a representative particulate air pollution mixture throughout gestation using a refined exposure model, and offspring response to allergen challenge was evaluated. After 4 weeks of chronic exposure to house dust mite allergen, offspring from both strains exposed to PM in utero demonstrated a reduced inflammatory response compared to filtered air controls. Airway hyperresponsiveness, a typical feature of asthma, was significantly different based on strain; however, air pollution exposure did not affect this response. In order to investigate the relevance of this model in an exposed human population, we conducted a pilot project (aim 3) evaluating exposure to particulate air pollution during pregnancy in a region in South Texas with a high incidence of childhood asthma. Results demonstrate low levels of air pollution exposure during pregnancy measured by personal sampling of fine particulate matter (PM2.5) and polycyclic aromatic hydrocarbons (PAHs). Overall, findings from this work lay a foundation for further clarifying the mechanisms underlying childhood respiratory disease resulting from early life air pollution exposure. Advisors/Committee Members: Johnson, Natalie M (advisor), Porter, Weston (advisor), Criscitiello, Michael (committee member), Harvey, Roger B (committee member).

Subjects/Keywords: air pollution; atopy; asthma

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rychlik, K. A. (2017). Prenatal Exposure to Particulate Air Pollution and Effects on Postnatal Immune Response. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161638

Chicago Manual of Style (16^th Edition):

Rychlik, Kristal Ann. “Prenatal Exposure to Particulate Air Pollution and Effects on Postnatal Immune Response.” 2017. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/161638.

MLA Handbook (7^th Edition):

Rychlik, Kristal Ann. “Prenatal Exposure to Particulate Air Pollution and Effects on Postnatal Immune Response.” 2017. Web. 03 Mar 2021.

Vancouver:

Rychlik KA. Prenatal Exposure to Particulate Air Pollution and Effects on Postnatal Immune Response. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/161638.

Council of Science Editors:

Rychlik KA. Prenatal Exposure to Particulate Air Pollution and Effects on Postnatal Immune Response. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161638

Texas A&M University

5. Mendoza Rodriguez, Maria G. Supplementation of Organic Acids and Algae Extracts in Aqua Feeds: Immunological Impacts.

Degree: MS, Wildlife and Fisheries Sciences, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151783

► Two organic acids, polyhydroxybutyrate (PHB) and potassium diformate (KDF) have been researched to only a limited extent with aquatic species but have been shown to… (more)

▼ Two organic acids, polyhydroxybutyrate (PHB) and potassium diformate (KDF) have been researched to only a limited extent with aquatic species but have been shown to have various positive effects on terrestrial animals. Two algae extracts, carrageenan and alginic acid, also have been shown to elicit immunostimulation in some fish. Therefore, the present study was conducted with red drum (Sciaenops ocellatus) as a model marine species to study the effects of organic acids and algae extracts as feed supplements by evaluating several humoral immune responses. Two feeding trials, one of 7-week duration and the other of 3-week were conducted with disease-free juvenile red drum (average initial wt. 2.6±0.2 g and 78.2 ±0.2 g, respectively). Semipurified diets were formulated to be isocaloric and contain 40% crude protein. Experimental diets were produced by supplementing the basal diet with KDF at 0.6%, PHB at 2%, alginic acid at 1% or carrageenan at 0.5% by weight in place of cellulose. Fish were stocked into 110-L aquaria operated as a recirculating system with each diet assigned to three replicate aquaria containing either 15 fish (7-week trial) or 9 fish per aquarium (3-week trial). All fish were fed their respective diets at the same fixed percentage of body weight (initially 6% and gradually reduced to 4% as the fish grew). Body weight was monitored by collectively weighing fish from each aquarium every week. At the end of each feeding trial, weight gain and feed efficiency were significantly (P<0.0001) reduced in fish feed PHB compared to the basal diet and both algae extracts. There were no significant differences in condition indices such as hepatosomatic index (HSI) and intraperitoneal fat (IPF) ratio among fish fed the various diets. Lysozyme activity was significantly higher in fish fed alginic acid. The greatest phagocytic activity was found in fish fed the diet containing PHB. Total immunoglobulin level was higher in fish fed the diet supplemented with carrageenan. Goblet cell proliferation was greatest in the posterior end of the gastrointestinal tract but not different among dietary treatments. Organic acids and algae extracts evaluated in this study produced variable immunological responses in red drum with carrageenan showing the greatest potential as an immunostimulant. Advisors/Committee Members: Gatlin III, Delbert M (advisor), Criscitiello, Michael (committee member), Hume, Michael (committee member).

Subjects/Keywords: red drum; organic acids; algae extracts; immunology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendoza Rodriguez, M. G. (2013). Supplementation of Organic Acids and Algae Extracts in Aqua Feeds: Immunological Impacts. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151783

Chicago Manual of Style (16^th Edition):

Mendoza Rodriguez, Maria G. “Supplementation of Organic Acids and Algae Extracts in Aqua Feeds: Immunological Impacts.” 2013. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/151783.

MLA Handbook (7^th Edition):

Mendoza Rodriguez, Maria G. “Supplementation of Organic Acids and Algae Extracts in Aqua Feeds: Immunological Impacts.” 2013. Web. 03 Mar 2021.

Vancouver:

Mendoza Rodriguez MG. Supplementation of Organic Acids and Algae Extracts in Aqua Feeds: Immunological Impacts. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/151783.

Council of Science Editors:

Mendoza Rodriguez MG. Supplementation of Organic Acids and Algae Extracts in Aqua Feeds: Immunological Impacts. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151783

Texas A&M University

6. Mashoof, Sara. Evolution of Mucosal Immunoglobulins: Xenopus Laevis IgX and Thunnus Orientalis IgZ/T.

Degree: PhD, Veterinary Microbiology, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/152474

► Despite a large number of studies during the last decade that investigated mucosal immunity in humans, very few works have been done on this immune… (more)

▼ Despite a large number of studies during the last decade that investigated mucosal immunity in humans, very few works have been done on this immune compartment in lower vertebrates. In the following two studies we focused on the mucosal immunoglobulins in two important species of two classes of ectothermic vertebrates: amphibians and bony fishes. Many studies address the influence of the gut microbiome on the immune system, but few dissect the effect of T cells on gut microbiota and mucosal responses. We have employed larval thymectomy in Xenopus to study the gut microbiota with and without the influence of T lymphocytes. Pyrosequencing of 16S ribosomal RNA genes was used to assess the relative abundance of bacterial groups present in the stomach, and the small and large intestine. Clostridiaceae were the most abundant family throughout the gut, while Bacteroidaceae, Enterobacteriaceae, and Flavobacteriaceae also were well represented. Unifrac analysis revealed no differences in microbiota distribution between thymectomized and unoperated frogs. This is consistent with immunization data showing that levels of the mucosal immunoglobulin IgX are not altered significantly by thymectomy. This study in Xenopus represents the oldest organisms that exhibit class switch to a mucosal isotype and is relevant to mammalian immunology, as IgA appears to have evolved from IgX based upon phylogeny, genomic synteny, and function. It is now appreciated that in addition to the immunoglobulin (Ig)M and D isotypes fish also make the mucosal IgT. In this study we sequenced the full length of Ig tau as well as mu in the commercially important Thunnus orientalis (Pacific bluefin tuna), the first analysis of both of these Ig isotypes in a member of the order Perciformes. Tuna IgM and IgT are each composed of four constant (CH) domains. We cloned and sequenced 48 different variable (VH) domain rearrangements of tuna immunoglobulins and grouped the VH gene sequences to four VH gene segment families based on 70% nucleotide identity. Three VH gene families were used by both IgM and IgT but one group was only found to be used by IgM. Most interestingly, both Ig mu and Ig tau clones appear to use the same diversity (DH) segment, unlike what has been described in other species, although they have dedicated IgT and IgM joining (JH) gene segments. We complemented this repertoire study with phylogenetic and tissue expression analysis. In addition to supporting the development of humoral vaccines in this important aquaculture species, these data suggest that the DH-JH recombination rather than the VH-DH recombination may be instructive for IgT versus IgM/D bearing lymphocyte lineages in some fish. Advisors/Committee Members: Criscitiello, Michael (advisor), Tizard, Ian (committee member), Berghman, Luc (committee member), Rivera, Gonzalo (committee member).

Subjects/Keywords: Xenopus Laevis; Immunoglobulin; Tuna; Mucosal Immunity; IgT

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mashoof, S. (2014). Evolution of Mucosal Immunoglobulins: Xenopus Laevis IgX and Thunnus Orientalis IgZ/T. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/152474

Chicago Manual of Style (16^th Edition):

Mashoof, Sara. “Evolution of Mucosal Immunoglobulins: Xenopus Laevis IgX and Thunnus Orientalis IgZ/T.” 2014. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/152474.

MLA Handbook (7^th Edition):

Mashoof, Sara. “Evolution of Mucosal Immunoglobulins: Xenopus Laevis IgX and Thunnus Orientalis IgZ/T.” 2014. Web. 03 Mar 2021.

Vancouver:

Mashoof S. Evolution of Mucosal Immunoglobulins: Xenopus Laevis IgX and Thunnus Orientalis IgZ/T. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/152474.

Council of Science Editors:

Mashoof S. Evolution of Mucosal Immunoglobulins: Xenopus Laevis IgX and Thunnus Orientalis IgZ/T. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/152474

Texas A&M University

7. Fisher, Colleen 1988-. Diversity and Evolution of the Bovine and Equine Toll-Like Receptor Gene Family: Applications to Animal Disease.

Degree: MS, Biomedical Sciences, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/148065

► Genes modulating innate immunity in mammals are generally considered the first line of defense with respect to invading pathogens and therefore it has become important… (more)

▼ Genes modulating innate immunity in mammals are generally considered the first line of defense with respect to invading pathogens and therefore it has become important to characterize naturally occurring genetic variation, and subsequently determine whether this variation is likely to be benign, beneficial, or detrimental to the host. Relevant to this study, the mammalian Toll-like receptor proteins (TLR), encoded by members of the TLR gene family, have the capacity to recognize a wide variety of pathogen ligands, and mutations within these genes have been shown to influence disease susceptibility or resistance within mammalian species. Two studies which sought to determine the frequency and distribution of naturally occurring genetic variation within the bovine and equine TLR genes revealed a large number of discrete point mutations, which were subsequently used to reconstruct haplotypes for each investigated gene across a large number of samples. Detailed analyses of haplotypes provided evidence for extensive haplotype sharing among specialized breeds, subspecies, and even divergent species. Classical and new tests of selection provided evidence for significant deviations from a strictly neutral model of molecular evolution for both cattle as well as equids, with some of the same TLR genes deviating from a strictly neutral model among divergent species. As a first step toward determining whether naturally occurring bovine TLR variation is likely to be benign, beneficial, or detrimental, we tested validated variation from bovine TLR genes capable of recognizing components of Mycobacteria for associations with Mycobacterium avium subspecies paratuberculosis (MAP) infection in dairy cattle, and found several SNPs that were nominally associated with disease status, thereby providing evidence for small-effect loci potentially influencing risk for differential susceptibility to Johne's disease. Advisors/Committee Members: Seabury, Christopher M (advisor), Criscitiello, Michael (committee member), Murphy, William (committee member).

Subjects/Keywords: Johne's Disease; Toll-like receptor; TLR; Horse Genomics; Cattle Genomics; Equine Genomics; Bovine Genomics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fisher, C. 1. (2012). Diversity and Evolution of the Bovine and Equine Toll-Like Receptor Gene Family: Applications to Animal Disease. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/148065

Chicago Manual of Style (16^th Edition):

Fisher, Colleen 1988-. “Diversity and Evolution of the Bovine and Equine Toll-Like Receptor Gene Family: Applications to Animal Disease.” 2012. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/148065.

MLA Handbook (7^th Edition):

Fisher, Colleen 1988-. “Diversity and Evolution of the Bovine and Equine Toll-Like Receptor Gene Family: Applications to Animal Disease.” 2012. Web. 03 Mar 2021.

Vancouver:

Fisher C1. Diversity and Evolution of the Bovine and Equine Toll-Like Receptor Gene Family: Applications to Animal Disease. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/148065.

Council of Science Editors:

Fisher C1. Diversity and Evolution of the Bovine and Equine Toll-Like Receptor Gene Family: Applications to Animal Disease. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/148065

Texas A&M University

8. Yu, Xue. Exploring the Molecular Interactions Between Host Cells and Cryptosporidium parvum During Invasion.

Degree: PhD, Veterinary Pathobiology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173901

► Cryptosporidium parvum is a zoonotic protozoan parasite belonging to the Phylum Apicomplexa and a causative agent of mild to severe watery diarrhea in humans and… (more)

▼ Cryptosporidium parvum is a zoonotic protozoan parasite belonging to the Phylum Apicomplexa and a causative agent of mild to severe watery diarrhea in humans and animals. The infection starts with the ingestion of oocysts, often from contaminated water, followed by the release of four sporozoites from individual oocysts in the small intestine and the invasion of sporozoites into intestinal epithelial cells. A unique, host cell-derived parasitophorous vacuole membrane (PVM) will be formed during the parasite invasion to contain the intracellular developing parasites. However, the essential molecular interactions between the parasite and host cells during infection and the mechanism of PVM formation are poorly understood. The study employed two approaches to study the molecular mechanisms of invasion by the C. parvum sporozoites. The first approach focused on identifying host cell factors, in which three host cell mutants generated with UV-irradiation-based mutagenesis have significantly increased resistance to the invasion by C. parvum sporozoites. One of the mutants was significantly resistant to the attachment of sporozoites onto host cells, and can be used to identify genes and pathways responsible for the parasite attachment by forward genetics. The second approach focused on a multifunctional parasite protein, which is discharged to the host cell surface during the parasite invasion. There was strong evidence indicating that this parasite protein participated in the aggregation of host cell filamentous actin (F-actin) and was associated with the formation of PVM. This study has not only generated new knowledge towards understanding the mechanisms of C. parvum infection, but also identified new directions to further explore the molecular pathways in the host cells and the parasites responsible for the parasite invasion. Advisors/Committee Members: Zhu, Guan (advisor), Criscitiello, Michael (committee member), Johnson, Gregory (committee member), Tian, Yanan (committee member).

Subjects/Keywords: Cryptosporidum parvum; invasion mechnisms; host cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, X. (2018). Exploring the Molecular Interactions Between Host Cells and Cryptosporidium parvum During Invasion. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173901

Chicago Manual of Style (16^th Edition):

Yu, Xue. “Exploring the Molecular Interactions Between Host Cells and Cryptosporidium parvum During Invasion.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/173901.

MLA Handbook (7^th Edition):

Yu, Xue. “Exploring the Molecular Interactions Between Host Cells and Cryptosporidium parvum During Invasion.” 2018. Web. 03 Mar 2021.

Vancouver:

Yu X. Exploring the Molecular Interactions Between Host Cells and Cryptosporidium parvum During Invasion. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/173901.

Council of Science Editors:

Yu X. Exploring the Molecular Interactions Between Host Cells and Cryptosporidium parvum During Invasion. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173901

Texas A&M University

9. Molaei, Maral. NF-ĸB/Relish and the Control of Cellular Triglyceride Metabolism in Drosophila Melanogaster.

Degree: PhD, Biomedical Sciences, 2019, Texas A&M University

URL: http://hdl.handle.net/1969.1/188779

► Metabolic and innate immune signaling pathways have co-evolved to elicit coordinated responses. However, dissecting the integration of these ancient signaling mechanisms remains a challenge. Using… (more)

Subjects/Keywords: NF-ĸB; Relish; FoxO; Lipolysis; Drosophila; Triglyceride

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Molaei, M. (2019). NF-ĸB/Relish and the Control of Cellular Triglyceride Metabolism in Drosophila Melanogaster. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/188779

Chicago Manual of Style (16^th Edition):

Molaei, Maral. “NF-ĸB/Relish and the Control of Cellular Triglyceride Metabolism in Drosophila Melanogaster.” 2019. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/188779.

MLA Handbook (7^th Edition):

Molaei, Maral. “NF-ĸB/Relish and the Control of Cellular Triglyceride Metabolism in Drosophila Melanogaster.” 2019. Web. 03 Mar 2021.

Vancouver:

Molaei M. NF-ĸB/Relish and the Control of Cellular Triglyceride Metabolism in Drosophila Melanogaster. [Internet] [Doctoral dissertation]. Texas A&M University; 2019. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/188779.

Council of Science Editors:

Molaei M. NF-ĸB/Relish and the Control of Cellular Triglyceride Metabolism in Drosophila Melanogaster. [Doctoral Dissertation]. Texas A&M University; 2019. Available from: http://hdl.handle.net/1969.1/188779

Texas A&M University

10. Ott, Jeannine Annette. The Elastic Elasmobranch: Nurse Sharks Commission B Cell Components and Somatic Hypermutation Mechanisms to Diversify T Cells During Thymic Development.

Degree: PhD, Biomedical Sciences, 2020, Texas A&M University

URL: http://hdl.handle.net/1969.1/191808

► Since the discovery of the T cell receptor (TCR) in 1983, immunologists have assigned somatic hypermutation (SHM) as a mechanism employed solely by B cells… (more)

▼ Since the discovery of the T cell receptor (TCR) in 1983, immunologists have assigned somatic hypermutation (SHM) as a mechanism employed solely by B cells to diversify their antigen receptors. Remarkably, we found SHM acting in the thymus on the α chain locus of shark TCR for TCR repertoire generation. SHM in developing shark T cells likely is catalyzed by activation‐induced cytidine deaminase (AID) and results in both point and tandem mutations that accumulate non‐conservative amino acid replacements within complementarity‐determining regions (CDRs). Mutation frequency at TCRα was as high as that seen at B cell receptor loci (BCR) in sharks and mammals, and the mechanism of SHM shares unique characteristics first detected at shark BCR loci. Additionally, fluorescence in situ hybridization showed the strongest AID expression in thymic corticomedullary junction and medulla. We suggest that TCRα utilizes SHM to broaden diversification of the primary αβ T cell repertoire in sharks, the first reported use of this process in thymic diversification in vertebrates. In addition to canonical T and B cell receptors, cartilaginous fish assemble non‐ canonical TCR that employ various B cell components. For example, shark T cells associate alpha (TCR‐alpha) or delta (TCR‐delta) constant (C) regions with immunoglobulin (Ig) heavy chain (H) variable (V) segments or TCR‐associated Ig‐like V (TAIL V) segments to form chimeric IgHV‐T cell receptors, and combine TCR δC with both Ig‐like and TCR‐like V segments to form the doubly‐rearranging NAR‐TCR. Here, we found that the use of SHM by nurse shark TCR varies depending on the particular V segment or C region used. First, SHM significantly alters alpha/delta V (TCR αδV) segments using TCR αC but not TCR δC. Second, mutation to IgHV segments associated with TCR δC was reduced compared to mutation to TCR αδV associated with TCR αC. Mutation was present but limited in V segments of all other TCR chains, including NAR‐TCR. Unexpectedly, we found preferential rearrangement of the non‐canonical IgHV‐TCR δC over canonical TCR αδV‐ TCR δC receptors. The differential use of SHM may reveal how AID targets V regions. Advisors/Committee Members: Criscitiello, Michael F (advisor), Raudsepp, Terje (committee member), Riley, David (committee member), Ghaffari, Noushin (committee member).

Subjects/Keywords: T cell receptor; TCRA/TCRD locus; somatic hypermutation; shark; thymus

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ott, J. A. (2020). The Elastic Elasmobranch: Nurse Sharks Commission B Cell Components and Somatic Hypermutation Mechanisms to Diversify T Cells During Thymic Development. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/191808

Chicago Manual of Style (16^th Edition):

Ott, Jeannine Annette. “The Elastic Elasmobranch: Nurse Sharks Commission B Cell Components and Somatic Hypermutation Mechanisms to Diversify T Cells During Thymic Development.” 2020. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/191808.

MLA Handbook (7^th Edition):

Ott, Jeannine Annette. “The Elastic Elasmobranch: Nurse Sharks Commission B Cell Components and Somatic Hypermutation Mechanisms to Diversify T Cells During Thymic Development.” 2020. Web. 03 Mar 2021.

Vancouver:

Ott JA. The Elastic Elasmobranch: Nurse Sharks Commission B Cell Components and Somatic Hypermutation Mechanisms to Diversify T Cells During Thymic Development. [Internet] [Doctoral dissertation]. Texas A&M University; 2020. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/191808.

Council of Science Editors:

Ott JA. The Elastic Elasmobranch: Nurse Sharks Commission B Cell Components and Somatic Hypermutation Mechanisms to Diversify T Cells During Thymic Development. [Doctoral Dissertation]. Texas A&M University; 2020. Available from: http://hdl.handle.net/1969.1/191808

Texas A&M University

11. Halley-Schultz, Yvette A. A Draft De Novo Genome Assembly and Mitochondrial Population Genomics for the Northern Bobwhite (Colinus Virginianus).

Degree: PhD, Genetics, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/156517

► Wild populations of northern bobwhites (Colinus virginianus; hereafter bobwhite) have declined across most of their historic U.S. range, and despite their importance as an experimental… (more)

▼ Wild populations of northern bobwhites (Colinus virginianus; hereafter bobwhite) have declined across most of their historic U.S. range, and despite their importance as an experimental wildlife model for ecotoxicology studies, no bobwhite draft genome assembly has emerged. Herein, we present the first bobwhite draft de novo genome assembly, with more than 90% of the assembled bobwhite genome captured within < 40,000 final scaffolds (N50 = 45.4 Kb) despite evidence for approximately 3.22 heterozygous polymorphisms per Kb. Moreover, three annotation analyses produced evidence for > 14,000 unique genes and proteins. Bobwhite analyses of divergence with the chicken (Gallus gallus) and zebra finch (Taeniopygia guttata) genomes revealed many extremely conserved gene sequences, and evidence for lineage-specific divergence of noncoding regions. Coalescent models for reconstructing the demographic history of the bobwhite and the scarlet macaw were concordant with how opposing natural selection strategies (i.e., skewness in the r-/K-selection continuum) would be expected to shape genome diversity and the effective population sizes in these species. Using genomic tools and resources developed via the draft genome assembly, we evaluated the concordance of population inferences and conclusions resulting from the analysis of short mitochondrial fragments (i.e., partial or complete D-Loop nucleotide sequences) versus complete mitogenome sequences for 53 bobwhites representing six ecoregions across TX and OK (USA). Median joining (MJ) haplotype networks demonstrated that analyses performed using small mitochondrial fragments were insufficient for estimating the true (i.e., complete) mitogenome haplotype structure, corresponding levels of divergence, and maternal population history of our samples. Notably, discordant demographic inferences were observed when mismatch distributions of partial (i.e., partial D-Loop) versus complete mitogenome sequences were compared, with the reduction in mitochondrial genomic information content observed to encourage spurious inferences in our samples. A probabilistic approach to variant prediction for the complete bobwhite mitogenomes revealed 344 segregating sites corresponding to 347 total mutations, including 49 putative nonsynonymous single nucleotide variants (SNVs) distributed across 12 protein coding genes. Evidence of gross heteroplasmy was observed for 13 bobwhites, with 10 of the 13 heteroplasmies involving one moderate to high frequency SNV. Haplotype network and phylogenetic analyses for the complete bobwhite mitogenome sequences revealed two divergent maternal lineages (dXY = 0.00731; FST = 0.849; P < 0.05), thereby supporting the potential for two putative subspecies. However, the diverged lineage (n = 103 variants) almost exclusively involved bobwhites geographically classified as Colinus virginianus texanus, which is discordant with the expectations of previous geographic subspecies designations. Tests of adaptive evolution for functional divergence (MKT), frequency distribution… Advisors/Committee Members: Seabury, Christopher M (advisor), Murphy, William J (committee member), Criscitiello, Michael F (committee member), Womack, James E (committee member).

Subjects/Keywords: Genomics; Genetics; northern bobwhite

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Halley-Schultz, Y. A. (2015). A Draft De Novo Genome Assembly and Mitochondrial Population Genomics for the Northern Bobwhite (Colinus Virginianus). (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156517

Chicago Manual of Style (16^th Edition):

Halley-Schultz, Yvette A. “A Draft De Novo Genome Assembly and Mitochondrial Population Genomics for the Northern Bobwhite (Colinus Virginianus).” 2015. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/156517.

MLA Handbook (7^th Edition):

Halley-Schultz, Yvette A. “A Draft De Novo Genome Assembly and Mitochondrial Population Genomics for the Northern Bobwhite (Colinus Virginianus).” 2015. Web. 03 Mar 2021.

Vancouver:

Halley-Schultz YA. A Draft De Novo Genome Assembly and Mitochondrial Population Genomics for the Northern Bobwhite (Colinus Virginianus). [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/156517.

Council of Science Editors:

Halley-Schultz YA. A Draft De Novo Genome Assembly and Mitochondrial Population Genomics for the Northern Bobwhite (Colinus Virginianus). [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156517

Texas A&M University

12. Martin, Cameron Lee. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.

Degree: MS, Biomedical Sciences, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/161578

► Lack of safe and effective adjuvants is a major hindrance to the development of efficacious vaccines. Signaling via CD40 pathway leads to enhanced antigen processing… (more)

Subjects/Keywords: Agonistic anti-CD40 mAb; Ovine; Caprine; Bovine; Vaccines; Adjuvant

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martin, C. L. (2017). Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161578

Chicago Manual of Style (16^th Edition):

Martin, Cameron Lee. “Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.” 2017. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/161578.

MLA Handbook (7^th Edition):

Martin, Cameron Lee. “Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.” 2017. Web. 03 Mar 2021.

Vancouver:

Martin CL. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/161578.

Council of Science Editors:

Martin CL. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161578

Texas A&M University

13. Vuong, Christine Nguyen. Antibody-guided Complexes and Their Potential Applications in Poultry Research.

Degree: PhD, Veterinary Pathobiology, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/173058

► Targeting the CD40 receptor displayed by antigen-presenting cells to deliver a specific immunogen has been successfully used to enhance immune responses, specifically increasing antibody production… (more)

▼ Targeting the CD40 receptor displayed by antigen-presenting cells to deliver a specific immunogen has been successfully used to enhance immune responses, specifically increasing antibody production and enhancing antibody affinity. When tested in chickens, this platform induced specific IgG and IgA production within one week post-immunization. However, proof of conferred protective efficacy using the CD40-targeting vaccination method was still undetermined. Whole avian influenza virus was loaded onto the guided complex and immunized birds were challenged with highly pathogenic avian influenza (HPAI) to test efficacy. Furthermore, this research addresses the application of guided complexes as an alternative method for epitope mapping of microbial enzymes. Short peptide segments of the Clostridium perfringens alpha toxin were loaded onto the antibody-guided complex and immunized into chickens to induce antibody production for downstream use in neutralization assays to identify specific regions able to block the toxin’s enzymatic functions. Lastly, to expand the antibody-guided system repertoire, monoclonal antibodies against a new receptor, specifically dendritic cell (DC) marker CD205, were developed for potential use to further enhance immune response activation. Anti-CD205 monoclonal antibodies were used to develop a new in vitro DC system obtained from peritoneal exudate cells. In HPAI efficacy studies, functional antibody titers were detected up to six weeks after a single subcutaneous administration. When boosted, the antibody-guided complex conferred 100% protection in birds upon lethal H5N1 challenge. The guided system also proved useful for rapid polyclonal antibody production in chickens, which can be used in epitope mapping studies. This system favors linear peptide targets for immunization in order to maintain cost-effectiveness and short turnover time, but can still be used with conformational epitopes. Monoclonal antibodies were successfully constructed against chicken CD205 and used in a variety of immunoassays, as well as magnetic bead isolation of DCs from peritoneal exudate cell populations. Overall, these data are the first to report protective efficacy using the CD40-targeting system in chickens, the first to propose the use of guided complexes in epitope mapping, and the first to isolate DCs from peritoneal exudate using the anti-CD205 monoclonal antibodies. Advisors/Committee Members: Berghman, Luc R (advisor), Criscitiello, Michael F (committee member), Caldwell, David J (committee member), Bielke, Lisa R (committee member), Zhang, Shuping (committee member).

Subjects/Keywords: antibody-guided; CD40; CD205; alpha toxin; avian influenza; antigen presenting cells; dendritic cells; poultry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vuong, C. N. (2017). Antibody-guided Complexes and Their Potential Applications in Poultry Research. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173058

Chicago Manual of Style (16^th Edition):

Vuong, Christine Nguyen. “Antibody-guided Complexes and Their Potential Applications in Poultry Research.” 2017. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/173058.

MLA Handbook (7^th Edition):

Vuong, Christine Nguyen. “Antibody-guided Complexes and Their Potential Applications in Poultry Research.” 2017. Web. 03 Mar 2021.

Vancouver:

Vuong CN. Antibody-guided Complexes and Their Potential Applications in Poultry Research. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/173058.

Council of Science Editors:

Vuong CN. Antibody-guided Complexes and Their Potential Applications in Poultry Research. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/173058

Texas A&M University

14. Breaux, Breanna Laine. The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution.

Degree: PhD, Veterinary Pathobiology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173818

► The adaptive immune system is responsible for an antigen-specific response that is stronger and faster with each subsequent infection. The hallmarks of this response are… (more)

▼ The adaptive immune system is responsible for an antigen-specific response that is stronger and faster with each subsequent infection. The hallmarks of this response are the B cell and the T cell, which are responsible for recognizing foreign antigens through their highly diverse receptors: the immunoglobulin (Ig) and T cell receptor (TR). These receptors have been studied in many mammalian species, however there are still gaps in our knowledge throughout mammalian evolution. Two eutherian sister superorders, Afrotheria and Xenarthra, have been neglected in this field of research. Therefore, we chose to characterize the adaptive immune receptor genes of the Florida manatee (Trichechus manatus latirostris), the only afrotherian species that inhabits the United States. We annotated the adaptive immune receptor loci in the genomic scaffolds of the Florida manatee genome and sequenced expressed transcripts using PacBio SMRT sequencing. In the IgH locus, we found limited IgHV diversity. The IgHV segments were limited in number and sequence diversity; the Florida manatee lacked clan III IgHV segments, which are conserved throughout most mammals. The loss of clan III IgHV consistently correlated to a decrease in the number of functional IgHV in cattle, sheep, and horse. This shared phenomenon in an evolutionarily distinct species highlighted the role of the clan III IgHV segments in maintaining locus diversity. Overall, the three TR loci had average to above average diversity. We separated the Florida manatee TRV segments into subgroups based on 75% nucleotide identity and identified conserved subgroups and subgroup order compared to the human TR loci. We identified a direct correlation between locus complexity, TRV sequence conservation, and locus synteny. This revealed the magnitude of overall maintenance of each TR locus across two divergent eutherian mammals for the first time. It also emphasized the role of locus organization on gene evolution. By including this understudied eutherian superorder into the analysis of these complex genes, we identified new evolutionary patterns that help us understand the factors that shaped our immune response. Advisors/Committee Members: Criscitiello, Michael F (advisor), Berghman, Luc R (committee member), Tizard, Ian R (committee member), Hurtado, Luis A (committee member).

Subjects/Keywords: immunoglobulin; t cell receptor; manatee; clan; synteny

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Breaux, B. L. (2018). The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173818

Chicago Manual of Style (16^th Edition):

Breaux, Breanna Laine. “The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/173818.

MLA Handbook (7^th Edition):

Breaux, Breanna Laine. “The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution.” 2018. Web. 03 Mar 2021.

Vancouver:

Breaux BL. The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/173818.

Council of Science Editors:

Breaux BL. The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173818

Texas A&M University

15. Breaux, Breanna Laine. The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution.

Degree: PhD, Veterinary Pathobiology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173723

► The adaptive immune system is responsible for an antigen-specific response that is stronger and faster with each subsequent infection. The hallmarks of this response are… (more)

▼ The adaptive immune system is responsible for an antigen-specific response that is stronger and faster with each subsequent infection. The hallmarks of this response are the B cell and the T cell, which are responsible for recognizing foreign antigens through their highly diverse receptors: the immunoglobulin (Ig) and T cell receptor (TR). These receptors have been studied in many mammalian species, however there are still gaps in our knowledge throughout mammalian evolution. Two eutherian sister superorders, Afrotheria and Xenarthra, have been neglected in this field of research. Therefore, we chose to characterize the adaptive immune receptor genes of the Florida manatee (Trichechus manatus latirostris), the only afrotherian species that inhabits the United States. We annotated the adaptive immune receptor loci in the genomic scaffolds of the Florida manatee genome and sequenced expressed transcripts using PacBio SMRT sequencing. In the IgH locus, we found limited IgHV diversity. The IgHV segments were limited in number and sequence diversity; the Florida manatee lacked clan III IgHV segments, which are conserved throughout most mammals. The loss of clan III IgHV consistently correlated to a decrease in the number of functional IgHV in cattle, sheep, and horse. This shared phenomenon in an evolutionarily distinct species highlighted the role of the clan III IgHV segments in maintaining locus diversity. Overall, the three TR loci had average to above average diversity. We separated the Florida manatee TRV segments into subgroups based on 75% nucleotide identity and identified conserved subgroups and subgroup order compared to the human TR loci. We identified a direct correlation between locus complexity, TRV sequence conservation, and locus synteny. This revealed the magnitude of overall maintenance of each TR locus across two divergent eutherian mammals for the first time. It also emphasized the role of locus organization on gene evolution. By including this understudied eutherian superorder into the analysis of these complex genes, we identified new evolutionary patterns that help us understand the factors that shaped our immune response. Advisors/Committee Members: Criscitiello, Michael F (advisor), Berghman, Luc R (committee member), Tizard, Ian R (committee member), Hurtado, Luis A (committee member).

Subjects/Keywords: immunoglobulin; t cell receptor; manatee; clan; synteny

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Breaux, B. L. (2018). The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173723

Chicago Manual of Style (16^th Edition):

Breaux, Breanna Laine. “The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/173723.

MLA Handbook (7^th Edition):

Breaux, Breanna Laine. “The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution.” 2018. Web. 03 Mar 2021.

Vancouver:

Breaux BL. The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/173723.

Council of Science Editors:

Breaux BL. The Florida Manatee Adaptive Immune Receptor Genes Reveal Novel Patterns in Mammalian Evolution. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173723

Texas A&M University

16. Herlihy, Sarah E. Linking Two Seemingly Unrelated Diseases, Cancer and Acute Respiratory Distress Syndrome, Through a Dictyostelium Secreted Protein.

Degree: PhD, Biology, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153282

► The work in this dissertation links two diseases through a protein secreted by Dictyostelium discoideum cells. The protein, AprA, inhibits cell proliferation and induces chemorepulsion… (more)

▼ The work in this dissertation links two diseases through a protein secreted by Dictyostelium discoideum cells. The protein, AprA, inhibits cell proliferation and induces chemorepulsion (movement away) of Dictyostelium cells. This has implications in both cancer research and the study of Acute Respiratory Distress Syndrome. Cancer is a misregulation of cellular proliferation. Often the removal of a primary tumor results in rapid metastatic cell proliferation. The rapid proliferation of metastatic cells indicates the presence of a factor, called a chalone, secreted by the primary tumor cells, that inhibits metastatic cell proliferation. The ability of AprA to inhibit proliferation of the cells that secretes it classifies it as a chalone. Using the model organism Dictyostelium and the protein AprA allows us to study chalone signaling mechanisms. Acute Respiratory Distress Syndrome (ARDS) is characterized by an excess influx of neutrophils into the lungs. Neutrophils damage the lung tissue and ultimately recruit more neutrophils that repeat the process. A need exists to remove these cells and allow resolution to occur. One way to accomplish this is through chemorepulsion, the directional movement of cells away from an external cue. We can use AprA to study the mechanisms of chemorepulsion. In this dissertation, I have found that the PTEN-like protein CnrN, which is an inhibitor of proliferation and chemotaxis, is involved in both AprA proliferation inhibition and chemorepulsion of Dictyostelium cells. I have shown that the human protein DPPIV, which is structurally similar to AprA, causes chemorepulsion of human neutrophils. Additionally, aspirated DPPIV reduces the accumulation of neutrophils in the lungs of a mouse model of ARDS. Work shown in the appendices suggests that AprA signals through specific G protein-coupled receptors. The work in this dissertation studies the role of chalones and chemorepellents. It allows the unique opportunity to study chemorepulsion in both Dictyostelium and human cells. The hope and goal is that the work in this dissertation could lead to novel therapies for diseases such as cancer and ARDS. Advisors/Committee Members: Gomer, Richard H (advisor), Polymenis, Michael (committee member), Garcia, L Rene (committee member), Ryan, Kathryn (committee member), Criscitiello, Michael (committee member).

Subjects/Keywords: chemorepulsion; AprA; Dictyostelium; DPPIV; chalone

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Herlihy, S. E. (2014). Linking Two Seemingly Unrelated Diseases, Cancer and Acute Respiratory Distress Syndrome, Through a Dictyostelium Secreted Protein. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153282

Chicago Manual of Style (16^th Edition):

Herlihy, Sarah E. “Linking Two Seemingly Unrelated Diseases, Cancer and Acute Respiratory Distress Syndrome, Through a Dictyostelium Secreted Protein.” 2014. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/153282.

MLA Handbook (7^th Edition):

Herlihy, Sarah E. “Linking Two Seemingly Unrelated Diseases, Cancer and Acute Respiratory Distress Syndrome, Through a Dictyostelium Secreted Protein.” 2014. Web. 03 Mar 2021.

Vancouver:

Herlihy SE. Linking Two Seemingly Unrelated Diseases, Cancer and Acute Respiratory Distress Syndrome, Through a Dictyostelium Secreted Protein. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/153282.

Council of Science Editors:

Herlihy SE. Linking Two Seemingly Unrelated Diseases, Cancer and Acute Respiratory Distress Syndrome, Through a Dictyostelium Secreted Protein. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153282

Texas A&M University

17. Adetunji, Shakirat Adeola. Seroprevalence of Anti-Borrelia Burgdorferi Antibodies in White-Tailed Deer (Odocoileus Virginianus) from Texas.

Degree: MS, Biomedical Sciences, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/174222

► Lyme Disease is caused by the bacterial pathogen Borrelia burgdorferi, and is transmitted by the tick-vector Ixodes scapularis. It is the most prevalent arthropod-borne disease… (more)

Subjects/Keywords: Borrelia burgdorferi; Ixodes scapularis; Lyme Disease; sero-reactivity; Texas; white-tailed deer

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Adetunji, S. A. (2016). Seroprevalence of Anti-Borrelia Burgdorferi Antibodies in White-Tailed Deer (Odocoileus Virginianus) from Texas. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/174222

Chicago Manual of Style (16^th Edition):

Adetunji, Shakirat Adeola. “Seroprevalence of Anti-Borrelia Burgdorferi Antibodies in White-Tailed Deer (Odocoileus Virginianus) from Texas.” 2016. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/174222.

MLA Handbook (7^th Edition):

Adetunji, Shakirat Adeola. “Seroprevalence of Anti-Borrelia Burgdorferi Antibodies in White-Tailed Deer (Odocoileus Virginianus) from Texas.” 2016. Web. 03 Mar 2021.

Vancouver:

Adetunji SA. Seroprevalence of Anti-Borrelia Burgdorferi Antibodies in White-Tailed Deer (Odocoileus Virginianus) from Texas. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/174222.

Council of Science Editors:

Adetunji SA. Seroprevalence of Anti-Borrelia Burgdorferi Antibodies in White-Tailed Deer (Odocoileus Virginianus) from Texas. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/174222

18. Pollard, Dana Alicia. Molecular Assessment of North American Vector-Borne Hemoparasites and In Vitro Studies.

Degree: PhD, Veterinary Pathobiology, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/161303

► The hemoparasite Cytauxzoon felis causes cytauxzoonosis, an emerging infectious disease of cats in the United States. Its life cycle involves sexual reproduction in the tick… (more)

▼ The hemoparasite Cytauxzoon felis causes cytauxzoonosis, an emerging infectious disease of cats in the United States. Its life cycle involves sexual reproduction in the tick vector and asexual reproduction (schizogonous and erythrocyctic stages) in the felid. Cytauxzoon felis studies involve the use of infected cats, and in vitro cultivation would obviate this use as well as provide a continuous parasite source. Modeled after the microaerophilous stationary phase system used in cultures of Babesia bovis, a similar hemoparasite, 332 C. felis cultures were initiated to optimize the in vitro cultivation of the erythrocytic stage. Although no continuous cultivation was achieved, parasites were maintained short-term at a percentage of parasitized erythrocytes ranging from undetectable to 1.1% for two days to 140 days, with an average of 32 days. Five cultures underwent subculture four times, the maximum level achieved. Cytauxzoonosis can manifest itself as a recovered, chronic, or fatal infection. Historically, nearly all infected cats died, but as years passed, the number of cats that did survive increased, which may be attributed to different strains of Cytauxzoon felis. Internal transcribed spacer regions 1 and 2 (ITS1 and ITS2) were assessed for genetic variation and used for determining clinical outcome and spatial correlations. Even though the ITS1 and ITS2 regions are genetically diverse, they do not aid in understanding differences in C. felis strains, as there was no association with clinical outcome or geography. A potential C. felis coinfection with a hemoplasma was assessed for difference in clinical outcome. Polymerase chain reaction (PCR) and subsequent cloning showed clones of 11 of 41 C. felis-infected cats as positive for Candidatus Mycoplasma haemominutum with at least 99% identity and two other cats as positive for Rhodococcus spp. with at least a 97% identity. Clones of one of these two cats also matched Candidatus Mycoplasma kahanei with 99% identity. Based on prior molecular evidence of B. bovis and the sylvatic component of the C. felis life cycle, 161 ticks removed from white-tailed deer in south Texas were molecularly surveyed for hemoparasites. No ticks were positive for species of Babesia, Cytauxzoon, or Theileria. Advisors/Committee Members: Holman, Patricia (advisor), Criscitiello, Michael (committee member), Ball, Judith (committee member), Berghman, Luc (committee member).

Subjects/Keywords: Cytauxzoon felis; internal transcribed spacer region; 18S rRNA; culture; erythrocyte

1 more image…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pollard, D. A. (2017). Molecular Assessment of North American Vector-Borne Hemoparasites and In Vitro Studies. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161303

Chicago Manual of Style (16^th Edition):

Pollard, Dana Alicia. “Molecular Assessment of North American Vector-Borne Hemoparasites and In Vitro Studies.” 2017. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/161303.

MLA Handbook (7^th Edition):

Pollard, Dana Alicia. “Molecular Assessment of North American Vector-Borne Hemoparasites and In Vitro Studies.” 2017. Web. 03 Mar 2021.

Vancouver:

Pollard DA. Molecular Assessment of North American Vector-Borne Hemoparasites and In Vitro Studies. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/161303.

Council of Science Editors:

Pollard DA. Molecular Assessment of North American Vector-Borne Hemoparasites and In Vitro Studies. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161303

19. Mirhosseini, Negin. Molecular Detection and Characterization of Avian Bornavirus.

Degree: PhD, Veterinary Microbiology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9529

► Proventricular dilatation disease (PDD) was first recognized during an outbreak among captive macaws in the late 1970s. The disease, also known as proventricular dilatation syndrome… (more)

▼ Proventricular dilatation disease (PDD) was first recognized during an outbreak among captive macaws in the late 1970s. The disease, also known as proventricular dilatation syndrome or macaw wasting disease can occur in any psittacine but the most commonly affected birds are macaws, cockatoos and conures. The disease causes inflammation of the central, peripheral and autonomic nervous systems, as well as weight loss associated with regurgitation and the passage of undigested food in the feces. Although a viral etiology for PDD has been suspected for almost 40 years, the etiologic agent of the disease was unknown until lately. Recently we cultured a novel bornavirus from brain tissue from birds clinically diagnosed with PDD. This finding supports data from other groups who, in 2008, identified bornavirus sequences among birds suffering from PDD. It was reported that more 60 percent of PDD affected birds were infected with the new virus, designated avian bornavirus (ABV). ABV is a negative sense, single stranded RNA virus related to Borna disease virus (BDV). ABV isolates differ dramatically from BDV isolates in their level of genetic variation. Using polymerase chain reaction (PCR) assays, we were able to detect ABV in feces and tissue of PDD birds. We also detected ABV shedding from clinically healthy birds housed in aviaries with no history of the disease. We also determined the complete genome sequences of eight North American ABV isolates. Genotyping indicates that the majority of North American ABV isolates are genotype 4. We found one ABV, genotype 1, which is the first complete sequencing of this genotype. Moreover, we found ABV genotype 2, isolated from an apparently healthy cockatiel with no PDD clinical signs. In order to investigate whether this genotype is avirulent, the virus was grown in duck embryo fibroblasts and inoculated into two adult cockatiels by the oral and intramuscular routes. One bird developed clinical signs on day 33 and was euthanized on day 36. The second challenged bird developed clinical signs on day 41 and was euthanized on day 45. On necropsy, the proventriculus of both birds was slightly enlarged and microscopic examination showed lesions consistent with PDD in the brain, spinal cord, heart, adrenal gland and intestine. A control, uninoculated cockatiel was apparently healthy when euthanized on day 50. ABV2 is now the second ABV genotype to be formally shown to cause PDD. Advisors/Committee Members: Payne, Susan (advisor), Tizard, Ian (committee member), Leibowitz, Julian (committee member), Criscitiello, Michael (committee member).

Subjects/Keywords: PDD; ABV

13 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mirhosseini, N. (2012). Molecular Detection and Characterization of Avian Bornavirus. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9529

Chicago Manual of Style (16^th Edition):

Mirhosseini, Negin. “Molecular Detection and Characterization of Avian Bornavirus.” 2012. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9529.

MLA Handbook (7^th Edition):

Mirhosseini, Negin. “Molecular Detection and Characterization of Avian Bornavirus.” 2012. Web. 03 Mar 2021.

Vancouver:

Mirhosseini N. Molecular Detection and Characterization of Avian Bornavirus. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9529.

Council of Science Editors:

Mirhosseini N. Molecular Detection and Characterization of Avian Bornavirus. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9529

20. Romoser, Amelia Antonia. Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process.

Degree: PhD, Toxicology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10877

► Nanoparticles, while included in a growing number of consumer products, may pose risks to human health due to heavy metal leaching and/or the production of… (more)

▼ Nanoparticles, while included in a growing number of consumer products, may pose risks to human health due to heavy metal leaching and/or the production of reactive oxygen species following exposures. Subcellular mechanisms of action triggered as a result of exposure to various nanoparticles are still largely unexplored. In this work, an effort to elucidate such toxicological parameters was accomplished by evaluating oxidative stress generation, changes in gene and protein expression, and cell cycle status after low-dose exposures to a variety of metal and carbon-based nanomaterials in primary human dermal cells. Additionally, mitigation of nanoparticle toxicity via microencapsulation was investigated to assess the feasibility of utilizing nanomaterials in dermally implantable biosensor applications. Cellular immune and inflammatory processes were measured via qPCR and immunoblotting, which revealed gene and protein expression modulation along the NF-kappaB pathway after a variety of nanoparticle exposures. The role of immunoregulatory transcription factor NF-kappaB was examined in an oxidative stress context in cells exposed to a panel of nanoparticles, whereby glutathione conversion and modulation of oxidative stress proteins in normal and NF-kappaB knockdown human dermal fibroblasts were monitored. Results revealed decreased antioxidant response and corresponding increased levels of oxidative stress and cell death in exposed normal cells, compared to NF-kappaB incompetent cells. However, reactive oxygen species production was not an absolute precursor to DNA damage, which was measured by the comet assay, gamma-H2AX expression, and flow cytometry. Protein analysis revealed that map kinase p38, rather than p53, was involved in the halting of the cell cycle in S-phase after ZnO exposures, which caused DNA double strand breaks. Microencapsulation of fluorescent quantum dot nanoparticles, specifically, was utilized as a method to improve system functionality and surrounding cellular viability for the purpose of a dermal analyte detection assay. In vitro results indicated a functional localization of nanoparticles, as well as cessation of cellular uptake. Subsequently, cellular metabolism was unaffected over the range of time and concentrations tested in comparison to unencapsulated quantum dot treatments, indicating the usefulness of this technique in developing nanoparticle-driven biomedical applications. Advisors/Committee Members: Sayes, Christie M. (advisor), Criscitiello, Michael F. (advisor), McShane, Michael (committee member), Porter, Weston (committee member).

Subjects/Keywords: nanoparticles; metal oxide; quantum dot; fullerol; NF-kB; immunomodulation; ROS; oxidative stress; inflammatory response; DNA damage; microencapsulation

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Romoser, A. A. (2012). Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10877

Chicago Manual of Style (16^th Edition):

Romoser, Amelia Antonia. “Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process.” 2012. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10877.

MLA Handbook (7^th Edition):

Romoser, Amelia Antonia. “Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process.” 2012. Web. 03 Mar 2021.

Vancouver:

Romoser AA. Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10877.

Council of Science Editors:

Romoser AA. Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-10877

21. Jacobs, Natalie Jo. Why Maintain Light Chain Isotypes? The Influence of Heavy Chain Isotype and Complementary Determining Region Lengths upon Light Chain Isotype in Xenopus laevis.

Degree: MS, Biomedical Sciences, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/156468

► Different immunoglobulin (Ig) heavy chain (H) isotypes have distinct functions, but so far it is unclear if Ig light (L) chains follow the same pattern.… (more)

Subjects/Keywords: immunoglobulin; complementary determining region; Xenopus laevis

12 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jacobs, N. J. (2015). Why Maintain Light Chain Isotypes? The Influence of Heavy Chain Isotype and Complementary Determining Region Lengths upon Light Chain Isotype in Xenopus laevis. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156468

Chicago Manual of Style (16^th Edition):

Jacobs, Natalie Jo. “Why Maintain Light Chain Isotypes? The Influence of Heavy Chain Isotype and Complementary Determining Region Lengths upon Light Chain Isotype in Xenopus laevis.” 2015. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/156468.

MLA Handbook (7^th Edition):

Jacobs, Natalie Jo. “Why Maintain Light Chain Isotypes? The Influence of Heavy Chain Isotype and Complementary Determining Region Lengths upon Light Chain Isotype in Xenopus laevis.” 2015. Web. 03 Mar 2021.

Vancouver:

Jacobs NJ. Why Maintain Light Chain Isotypes? The Influence of Heavy Chain Isotype and Complementary Determining Region Lengths upon Light Chain Isotype in Xenopus laevis. [Internet] [Masters thesis]. Texas A&M University; 2015. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/156468.

Council of Science Editors:

Jacobs NJ. Why Maintain Light Chain Isotypes? The Influence of Heavy Chain Isotype and Complementary Determining Region Lengths upon Light Chain Isotype in Xenopus laevis. [Masters Thesis]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156468

22. Eltahan, Rana Abbas Khedr. Exploring the Glycolytic Enzymes, Glucose-6-phosphate isomerase (CpGPI) and Hexokinase (CpHK) as Potential Drug Targets in Cryptosporidium parvum.

Degree: PhD, Biomedical Sciences, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/173649

► Cryptosporidium parvum is a water-borne and food-borne apicomplexan pathogen. It is one of the top four diarrheal-causing pathogens in children under the age of five… (more)

▼ Cryptosporidium parvum is a water-borne and food-borne apicomplexan pathogen. It is one of the top four diarrheal-causing pathogens in children under the age of five in developing countries, and an opportunistic pathogen in immunocompromised individuals. The preventative measures are not fully effective with, nitazoxanide (NTZ), the only FDA-approved drug for use in immunocompetent individuals. Unlike other apicomplexans, C. parvum lacks Kreb’s cycle and cytochrome-based respiration, thus relying mainly on glycolysis to produce ATP. In this study, we characterized the primary biochemical features of the C. parvum glucose-6-phosphate isomerase (CpGPI) and determined its Michaelis constant towards fructose-6-phosphate (Kvm = 0.309 mM, Vvmax = 31.72 nmol/μg/min). We also discovered that ebselen, an organoselenium drug, was an inhibitor of CpGPI by high-throughput screening of 1,200 known drugs. Ebselen acted on CpGPI as an allosteric noncompetitive inhibitor (ICv50 = 8.33 μM), while complete inhibition of CpGPI activity was not achieved. Although ebselen is useful in studying the inhibition of CpGPI enzyme activity, further proof is needed to chemically and/or genetically validate CpGPI as a drug target. We also identified four drugs as CpHK inhibitors with micromolar level of anti-cryptospordial activities at concentrations nontoxic to the host cells (i.e., hexachlorphene, thimerosal, alexidine dihydrochloride and ebselen with ECv50 = 0.53, 1.77, 8.1 and 165 μM, respectively). The anti-CpHK activity of the four existing drugs provided us new reagents for studying the enzyme properties of the parasite hexokinase. We have previously observed that 2-deoxy-D-glucose (2DG) could inhibit both the enzyme activity of C. parvum hexokinase (CpHK) and the parasite growth in vitro. However, the action and fate of 2DG in C. parvum was not fully investigated. In the present study, we showed that, although 2DG could be phosphorylated by CpHK to form 2DG-6-phosphate (2DG6P), the anti-cryptosporidial activity of 2DG was mainly attributed to the action of 2DG on CpHK, rather than the action of 2DG or 2DG6P on the downstream enzyme CpGPI, nor 2DG6P on CpHK. These observations further supported the hypothesis that CpHK could serve as a drug target in the parasite. Advisors/Committee Members: Zhu, Guan (advisor), Johnson, Gregory (committee member), Criscitiello, Michael (committee member), Tian, Yanan (committee member).

Subjects/Keywords: : Apicomplexan; Cryptosporidium parvum; Glucose-6-phosphate isomerase (GPI); Ebselen; Hexokinase (HK): Drug target

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Eltahan, R. A. K. (2018). Exploring the Glycolytic Enzymes, Glucose-6-phosphate isomerase (CpGPI) and Hexokinase (CpHK) as Potential Drug Targets in Cryptosporidium parvum. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173649

Chicago Manual of Style (16^th Edition):

Eltahan, Rana Abbas Khedr. “Exploring the Glycolytic Enzymes, Glucose-6-phosphate isomerase (CpGPI) and Hexokinase (CpHK) as Potential Drug Targets in Cryptosporidium parvum.” 2018. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/173649.

MLA Handbook (7^th Edition):

Eltahan, Rana Abbas Khedr. “Exploring the Glycolytic Enzymes, Glucose-6-phosphate isomerase (CpGPI) and Hexokinase (CpHK) as Potential Drug Targets in Cryptosporidium parvum.” 2018. Web. 03 Mar 2021.

Vancouver:

Eltahan RAK. Exploring the Glycolytic Enzymes, Glucose-6-phosphate isomerase (CpGPI) and Hexokinase (CpHK) as Potential Drug Targets in Cryptosporidium parvum. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/173649.

Council of Science Editors:

Eltahan RAK. Exploring the Glycolytic Enzymes, Glucose-6-phosphate isomerase (CpGPI) and Hexokinase (CpHK) as Potential Drug Targets in Cryptosporidium parvum. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173649

23. Godbeer, Stacey Marie. Incidence of Mupirocin Resistance in Staphylococcus pseudintermedius Isolated from a Healthy Dog.

Degree: MS, Biomedical Sciences, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151339

► Mupirocin is a bacteriostatic antibiotic that is used to decolonize people who carry methicillin-resistant staphylococci, primarily methicillin-resistant Staphylococcus aureus (MRSA). Mupirocin reversibly binds to bacterial… (more)

▼ Mupirocin is a bacteriostatic antibiotic that is used to decolonize people who carry methicillin-resistant staphylococci, primarily methicillin-resistant Staphylococcus aureus (MRSA). Mupirocin reversibly binds to bacterial isoleucyl tRNA synthetase to disrupt protein synthesis. Resistance to mupirocin is due either to a point mutation to the ileS gene that encodes the isoleucyl tRNA synthetase, classified as low-level mupirocin resistance; or, bacteria may obtain a plasmid that carries the ileS2 gene encoding an alternate isoleucyl tRNA synthetase, conferring high-level resistance. Mupirocin resistance plasmids contain insertion sequence (IS) 257 repeats, into which the ileS2 gene is inserted. Such plasmids have been characterized by their IS257-ileS2 junctions in both S. aureus and, recently, in Staphylococcus pseudintermedius in a dog from Croatia. The primary goals of this study were to determine the prevalence of mupirocin resistance in isolates of S. pseudintermedius in Texas, to determine whether resistance was due to point mutations in the native ileS or due to carriage of mupirocin resistance plasmids, and to characterize the structure of the mupirocin resistance genes carried on plasmids. In this study, 572 S. pseudintermedius isolates, collected from veterinary patients from across Texas were screened for their susceptibility to low levels of mupirocin. Of these isolates, only one out of 572 (0.17%) tested positive for mupirocin resistance and was found by polymerase chain reaction (PCR), using previously published primers mupA and mupB, to have a 458 bp fragment and, with primers M1 and M2 to have a 237 bp fragment, indicating the presence of the high-level mupirocin resistance gene, ileS2. The arrangement of the IS257-ileS2 junctions was then analyzed by PCR and the products, bands at 1816 bp for primers ileS2-5’ and IS257R and at 1127 bp for primers ileS2-3’ and IS257F, which are consistent with the amplification pattern for an S2 plasmid, were cloned into a plasmid, pT7Blue, and sequenced for comparison to published sequences in GenBank. BLAST analyses in NCBI, comparing the isolate to recently published sequences for mupirocin-resistant S. pseudintermedius isolated from a dog with pyoderma in Croatia, indicate a 100% similarity to the upstream junction, JX186508, and 97% to the downstream junction, JX186509. Advisors/Committee Members: Lawhon, Sara D. (advisor), Criscitiello, Michael F. (committee member), Bissett, Wesley (committee member).

Subjects/Keywords: Mupirocin; Staphylococcus pseudintermedius

3 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Godbeer, S. M. (2013). Incidence of Mupirocin Resistance in Staphylococcus pseudintermedius Isolated from a Healthy Dog. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151339

Chicago Manual of Style (16^th Edition):

Godbeer, Stacey Marie. “Incidence of Mupirocin Resistance in Staphylococcus pseudintermedius Isolated from a Healthy Dog.” 2013. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/151339.

MLA Handbook (7^th Edition):

Godbeer, Stacey Marie. “Incidence of Mupirocin Resistance in Staphylococcus pseudintermedius Isolated from a Healthy Dog.” 2013. Web. 03 Mar 2021.

Vancouver:

Godbeer SM. Incidence of Mupirocin Resistance in Staphylococcus pseudintermedius Isolated from a Healthy Dog. [Internet] [Masters thesis]. Texas A&M University; 2013. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/151339.

Council of Science Editors:

Godbeer SM. Incidence of Mupirocin Resistance in Staphylococcus pseudintermedius Isolated from a Healthy Dog. [Masters Thesis]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151339

24. Du, Christina. Investigation of Immunoglobulin Heavy Chain Isotypes in an Ancestral Mucosal Immune Model.

Degree: MS, Laboratory Animal Medicine, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10140

► The importance of gut associated lymphoid tissues has been extensively reported in higher vertebrates, but less is known in lower vertebrates. In mammals immunoglobulin (Ig)A… (more)

▼ The importance of gut associated lymphoid tissues has been extensively reported in higher vertebrates, but less is known in lower vertebrates. In mammals immunoglobulin (Ig)A is the primary Ig of mucosal immunity. But no IgA has been identified in cold-blooded animals. In higher vertebrates, antigen must stimulate the lymphoid tissues in the intestines to elicit an IgA response, and cytokines from CD4 positive helper T cells are required for B cell switch. It is not known if this is the case in lower vertebrates, or if T cell help evolved before or after class switch recombination between functional antibody isotypes. My study will fill in these gaps in our knowledge by comparing oral antigen inoculation relative to intraperitoneal antigen inoculation in frogs (Xenopus sp.). Oral immunization is a novel approach to eliciting immune responses in Xenopus. I propose that IgX will increase with oral inoculation compared to intraperitoneal injection. This would be the first demonstration of class switch upon oral immunization to a mucosal isotype in the first vertebrates that employs higher vertebrate Ig heavy chain switch mechanism, which would shed light on the most fundamental aspects of our humoral adaptive immune system. Using a total Ig ELISA protocol, measuring total relative levels of IgM, there was no difference between the first three groups of orally immunized frogs compared to intraperitoneally immunized frogs. However, a response to serum IgX was seen in the first group. On the other hand, the refined Ag-specific ELISA protocol did present a significant increase in serum IgM response in frogs immunized systemically over orally immunized animals, but not an overall IgX response. Phylogenetic analysis suggests that, contrary to initial reports, IgA evolved from IgX. With consideration of entire constant region and individual constant domain analyses as well as synteny and function, we suggest new hypotheses of vertebrate antibody evolution to be tested as immunogenetic coverage of more species continues to expand. Advisors/Committee Members: Criscitiello, Michael (advisor), Lawhon, Sara (committee member), Welsh, Jane, Gresham, Vincent (committee member).

Subjects/Keywords: IgA; IgX; Xenopus; mucosal; immunization

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Du, C. (2012). Investigation of Immunoglobulin Heavy Chain Isotypes in an Ancestral Mucosal Immune Model. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10140

Chicago Manual of Style (16^th Edition):

Du, Christina. “Investigation of Immunoglobulin Heavy Chain Isotypes in an Ancestral Mucosal Immune Model.” 2012. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10140.

MLA Handbook (7^th Edition):

Du, Christina. “Investigation of Immunoglobulin Heavy Chain Isotypes in an Ancestral Mucosal Immune Model.” 2012. Web. 03 Mar 2021.

Vancouver:

Du C. Investigation of Immunoglobulin Heavy Chain Isotypes in an Ancestral Mucosal Immune Model. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10140.

Council of Science Editors:

Du C. Investigation of Immunoglobulin Heavy Chain Isotypes in an Ancestral Mucosal Immune Model. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-10140

25. Wright, Rachel. Characterization of RPGR Variants and Their Role in Inherited Retinal Degeneration.

Degree: PhD, Genetics, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9804

► Retinitis Pigmentosa (RP) refers to a group of inherited retinal dystrophies resulting from progressive photoreceptor degeneration and accumulation of intra-retinal pigment-like deposits. X-linked forms of… (more)

▼ Retinitis Pigmentosa (RP) refers to a group of inherited retinal dystrophies resulting from progressive photoreceptor degeneration and accumulation of intra-retinal pigment-like deposits. X-linked forms of RP are frequently caused by mutations in the retinitis pigmentosa GTPase regulator (RPGR) gene. The RPGR transcript undergoes complex alternative splicing to express both constitutive (RPGR^ex1-19) and RPGR^ORF15 variants. Although RPGR is thought to play a role in ciliary function, little is known about the physiological significance of expressing two distinct groups of variants. This study compares Rpgr^ex1-19 and Rpgr^ORF15 expression in developing photoreceptors using immunoblot analysis and immunohistochemistry, assesses ciliary affinity in adult photoreceptors by protein fractionation, examines Rpgr function in transgenic mouse models and identifies a novel Rpgr^ORF15 binding partner using a yeast two-hybrid screen. Our data reveal that Rpgr expression undergoes dynamic temporal regulation during retinal development and indicates variability in ciliary localization of Rpgr variants in adult photoreceptors. Utilization of distinct Rpgr variants during stages of photoreceptor development suggests independent roles. Further examination of Rpgr function using transgenic mouse models over-expressing either the Rpgr^ex1-19 or Rpgr^ORF15 variant reveals that despite normal ciliary localization, an excess of RPGR^ex1-19 results in atypical accumulation of Rpgr in photoreceptor outer segments, abnormal photoreceptor morphology and severe retinal degeneration. The data indicate that the constitutive variant cannot substitute for Rpgr function in photoreceptors and suggest that proper maintenance of the Rpgr isoform ratio is critical to photoreceptor viability. Using mouse retinal cDNA in a yeast two-hybrid screen with the C-terminus of the Rpgr^ORF15 variant, we identified a novel variant of whirlin as an interacting partner. Mutations in whirlin result in Usher syndrome, a disorder characterized by hearing loss and RP. RT-PCR and immunoblot analysis were used to confirm the presence of selected candidate partners in the retina and interaction was confirmed by pull-down assays and co-immunoprecipitation from retinal homogenate. Immunohistochemistry showed co-localization of RPGR and whirlin within photoreceptors and identified isoform specific localization of whirlin. These findings indicate that whirlin binds Rpgr^ORF15 and that this novel isoform may be required for photoreceptor function, thus providing a potential mechanism for the RP phenotype observed in Usher syndrome. Advisors/Committee Members: Criscitiello, Michael F. (advisor), Perkins, Brian (committee member), Ko, Gladys (committee member), Welsh, C. Jane (committee member).

Subjects/Keywords: RPGR; Retinitis Pigmentosa; RP; Usher Syndrome; Retinal Degeneration

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wright, R. (2012). Characterization of RPGR Variants and Their Role in Inherited Retinal Degeneration. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9804

Chicago Manual of Style (16^th Edition):

Wright, Rachel. “Characterization of RPGR Variants and Their Role in Inherited Retinal Degeneration.” 2012. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9804.

MLA Handbook (7^th Edition):

Wright, Rachel. “Characterization of RPGR Variants and Their Role in Inherited Retinal Degeneration.” 2012. Web. 03 Mar 2021.

Vancouver:

Wright R. Characterization of RPGR Variants and Their Role in Inherited Retinal Degeneration. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9804.

Council of Science Editors:

Wright R. Characterization of RPGR Variants and Their Role in Inherited Retinal Degeneration. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-08-9804

26. Zychowski, Katherine E. Calcium Montmorillonite for the Mitigation of Aflatoxicosis and Gastrointestinal Inflammation.

Degree: PhD, Toxicology, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153340

► Clays have been used for centuries as ‘ancient medicine’ for their therapeutic benefits. One particular clay, calcium montmorillonite, has historically been used as an anti-caking… (more)

▼ Clays have been used for centuries as ‘ancient medicine’ for their therapeutic benefits. One particular clay, calcium montmorillonite, has historically been used as an anti-caking agent in animal feeds, but has also demonstrated the ability to bind toxins and alleviate infectious diarrhea. The full breadth of therapeutic applications and molecular mechanisms of montmorillonite is still unknown. Therefore, the purpose of this research was to explore novel therapeutic applications for NovaSil (NS), a calcium montmorillonite clay to reduce the risk of aflatoxicosis in farm-raised fish and alleviate gastrointestinal inflammation and dysbiosis in a mouse model of Crohn’s disease (CD). Aflatoxin B_(1) (AFB_(1)) is a fungal mycotoxin that commonly contaminates corn and peanut crops. It is produced by the fungi Aspergillus flavus and A. parasiticus during times of drought or due to improper post-harvest storage. Aflatoxin B_(1) is known to cause hepatocellular carcinoma, immunosuppression and growth stunting in several species. Recently, incorporation of plant-based alternatives into feed for farm-raised fish has become a trend, thereby increasing the risk for mycotoxin contamination. Inexpensive strategies to reduce AFB_(1) exposure are needed. Calcium montmorillonite clay, which is both inexpensive and abundant, has a dioctahedral structure that is known to sequester AFB_(1) in its negatively-charged interlayer, thereby reducing systemic bioavailability. There is also some evidence to suggest that calcium montmorillonite clays may possess gastrointestinal anti-inflammatory properties. NovaSil was used as a strategy to reduce the effects of AFB_(1) in Nile tilapia (Oreochromis niloticus) and red drum (Scieanops ocellatus). Juvenile tilapia and red drum were dosed with AFB_(1) and NS over the course of 10 and 7 weeks, respectively. Additionally, proinflammatory cytokine-clay binding was characterized using isothermal analysis, X-ray diffraction (XRD) and transmission electron microscopy (TEM). Furthermore, a TNBS (2,4,6-Trinitrobenzenesulfonic acid)-colitis gastrointestinal mouse model was employed to study the anti-inflammatory properties of NS and its ability to protect the gut microbiome. Results suggest that NS can prevent aflatoxicosis in red drum at a 2% inclusion level over the course of 7 weeks. NovaSil also prevented some toxicity in Nile tilapia; however, these results were not significant. In vitro results also indicate that NS sorbs proinflammatory cytokines such as TNFα and IL-1β in its interlayers. Additionally, NS was found to reduce serum pro-inflammatory cytokine levels in TNBS-induced mice and reduce gut dysbiosis. These results could positively impact both human and animal populations with AFB_(1) exposure and/or chronic gastrointestinal inflammation. Advisors/Committee Members: Phillips, Timothy D (advisor), Criscitiello, Michael (committee member), Gatlin, Delbert M (committee member), Harvey, Roger (committee member).

Subjects/Keywords: clay; aflatoxin; fish; Crohn's disease; anti-inflammatory

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zychowski, K. E. (2014). Calcium Montmorillonite for the Mitigation of Aflatoxicosis and Gastrointestinal Inflammation. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153340

Chicago Manual of Style (16^th Edition):

Zychowski, Katherine E. “Calcium Montmorillonite for the Mitigation of Aflatoxicosis and Gastrointestinal Inflammation.” 2014. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/153340.

MLA Handbook (7^th Edition):

Zychowski, Katherine E. “Calcium Montmorillonite for the Mitigation of Aflatoxicosis and Gastrointestinal Inflammation.” 2014. Web. 03 Mar 2021.

Vancouver:

Zychowski KE. Calcium Montmorillonite for the Mitigation of Aflatoxicosis and Gastrointestinal Inflammation. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/153340.

Council of Science Editors:

Zychowski KE. Calcium Montmorillonite for the Mitigation of Aflatoxicosis and Gastrointestinal Inflammation. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153340

27. Lokhandwala, Shehnaz Taher. Development of Adenovirus-vectored Prototype Vaccines for African Swine Fever Virus and Bovine Viral Diarrhea Virus.

Degree: PhD, Veterinary Pathobiology, 2017, Texas A&M University

URL: http://hdl.handle.net/1969.1/161338

► The objective of this work was to develop adenovirus-vectored prototype vaccines against two pathogens, African Swine Fever Virus (ASFV) and Bovine Viral Diarrhea Virus (BVDV),… (more)

▼ The objective of this work was to develop adenovirus-vectored prototype vaccines against two pathogens, African Swine Fever Virus (ASFV) and Bovine Viral Diarrhea Virus (BVDV), which cause disease in two major livestock species, swine and cattle respectively. The African Swine Fever Virus is a transboundary animal pathogen that causes a lethal hemorrhagic fever in domestic pigs. Attempts to develop a vaccine for ASFV have failed thus far. This manuscript describes the use of recombinant adenovirus to deliver two unique formulations of ASFV antigens in swine (in two separate in-vivo studies) and the subsequent evaluation of the antigen-specific antibody and cellular responses induced. The robust antigen-specific immune responses observed in both studies are promising and their protective potential will be evaluated in future efficacy studies The Bovine Viral Diarrhea Virus is a globally prevalent pathogen that can cause severe diarrhea, respiratory disease, abortions and sometimes death in calves. Killed and modified live vaccines (MLV) for BVDV have been in use since the 1960s but are not effective due to lack of cross-protection and retention of immunosuppressive characteristics. This thesis also describes the use of the recombinant adenovirus vector to deliver a cocktail of multiple mosaic BVDV antigens in calves followed by the evaluation of protection conferred upon challenge. The prototype vaccine was more immunogenic and cross-protective (based on neutralizing antibodies) than a commercial MLV BVDV vaccine. Regarding protective efficacy, all calves immunized with prototype vaccine cleared the virus within a week post-challenge, whereas one calf that received the MLV vaccine still remained viremic. Future efficacy studies with diverse BVDV strains are required to validate the cross-protective potential of this prototype vaccine. Advisors/Committee Members: Mwangi, Waithaka (advisor), Berghman, Luc (committee member), Criscitiello, Michael (committee member), Reddy, Sanjay (committee member), Waghela, Suryakant D (committee member), Welsh, Jane (committee member).

Subjects/Keywords: Adenovirus vectors; vaccine; ASFV, BVDV; Immunology; T-cells

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lokhandwala, S. T. (2017). Development of Adenovirus-vectored Prototype Vaccines for African Swine Fever Virus and Bovine Viral Diarrhea Virus. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/161338

Chicago Manual of Style (16^th Edition):

Lokhandwala, Shehnaz Taher. “Development of Adenovirus-vectored Prototype Vaccines for African Swine Fever Virus and Bovine Viral Diarrhea Virus.” 2017. Doctoral Dissertation, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/161338.

MLA Handbook (7^th Edition):

Lokhandwala, Shehnaz Taher. “Development of Adenovirus-vectored Prototype Vaccines for African Swine Fever Virus and Bovine Viral Diarrhea Virus.” 2017. Web. 03 Mar 2021.

Vancouver:

Lokhandwala ST. Development of Adenovirus-vectored Prototype Vaccines for African Swine Fever Virus and Bovine Viral Diarrhea Virus. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/161338.

Council of Science Editors:

Lokhandwala ST. Development of Adenovirus-vectored Prototype Vaccines for African Swine Fever Virus and Bovine Viral Diarrhea Virus. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/161338

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations