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Texas A&M University
1.
Calhoun, Dana Marie.
A Reevaluation of the Taxonomy of the Mesocoelium monas Complex (Platyhelminthes: Digenea: Mesoceliidae).
Degree: MS, Wildlife and Fisheries Sciences, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9347 
► Specimens from the National History Museum, London, and from the United States National Parasite Collection, Beltsville, United States of America., which had all previously been…
(more)
▼ Specimens from the National History Museum, London, and from the United States National Parasite Collection, Beltsville, United States of America., which had all previously been identified as Mescoelium monas from a variety of definitive hosts (amphibians, reptiles and/or fish), were evaluated using all available literature and the body-type keys developed by Norman Dronen. None of these 85 putative
M. monas specimens appeared to fit the original description of
M. monas. There are likely multiple species of Mescoelium worldwide, and the
M. monas complex, as currently defined, is not a unified monospecific grouping. The present study leads to the conclusion that there can be no fewer than 9 species of Mescoelium (6 body types, 3 of which can be divided into 2 separate groups based on the median or submedian placement of the genital pore). The specimens of
M. monas I examined represented 3 of the 6 body types identified by Norman Dronen: the
M. monas body type, the
M. lanceatum body type, and the
M. pesteri body type. Because Mesocoelium is a difficult group within which to distinguish species, it may be expedient in endoparasitic surveys of amphibians, fish and reptiles to consider any specimen of Mesocoelium found to be
M. monas. Because Mesocoelium is a difficult group within which to distinguish species, it may be expedient in endoparasitic surveys of amphibians, fish and reptiles to consider any specimen of Mesocoelium found to be
M. monas. Subsequent reevalations must consider these key characteristics: genital pore placement, cecal length, vitelline follicle distribution, along with other supporting characteristics.
Advisors/Committee Members: Dronen, Norman (advisor), Neill, William (committee member), Craig, Thomas (committee member).
Subjects/Keywords: Trematode; Parasitology; Amphibian; Reptile
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APA (6th Edition):
Calhoun, D. M. (2012). A Reevaluation of the Taxonomy of the Mesocoelium monas Complex (Platyhelminthes: Digenea: Mesoceliidae). (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9347
Chicago Manual of Style (16th Edition):
Calhoun, Dana Marie. “A Reevaluation of the Taxonomy of the Mesocoelium monas Complex (Platyhelminthes: Digenea: Mesoceliidae).” 2012. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9347.
MLA Handbook (7th Edition):
Calhoun, Dana Marie. “A Reevaluation of the Taxonomy of the Mesocoelium monas Complex (Platyhelminthes: Digenea: Mesoceliidae).” 2012. Web. 18 Jan 2021.
Vancouver:
Calhoun DM. A Reevaluation of the Taxonomy of the Mesocoelium monas Complex (Platyhelminthes: Digenea: Mesoceliidae). [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9347.
Council of Science Editors:
Calhoun DM. A Reevaluation of the Taxonomy of the Mesocoelium monas Complex (Platyhelminthes: Digenea: Mesoceliidae). [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9347
Texas A&M University
2.
Neely, Wesley James.
A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles.
Degree: MS, Wildlife and Fisheries Sciences, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/173743
► In this study, the metazoan parasites of three species of freshwater turtles (the spiny softshell, Apalone spinifera, the common snapping turtle, Chelydra serpentina, and the…
(more)
▼ In this study, the metazoan parasites of three species of freshwater turtles (the spiny softshell, Apalone spinifera, the common snapping turtle, Chelydra serpentina, and the red-eared slider, Trachemys scripta elegans) were surveyed at 16 sites across the state of
Texas. A total of 42 species of metazoan parasites were recovered from 15 A. spinifera, nine C. serpentina, and 55 T. s. elegans, representing 16 new host-parasite associations and 17 new locality records. The synonymy of Acanthostomum nuevoleonensis by Brooks (1980) is refuted and the species is redescribed.
Two new species of monogenean worms in the genus Neopolystoma are reported, one from C. serpentina and A. spinifera and another from T. s. elegans. Through non-metric multidimensional scaling and analysis of similarities, A. spinifera was found to contain a significantly distinct parasite community from C. serpentina and T. s. elegans. A range of water parameters (ammonia, carbon dioxide, chloride, dissolved oxygen, hardness, nitrite, nitrate, pH, salinity, temperature, and turbidity) were recorded on each sampling trip and compared to parasite abundance and diversity. Ammonia levels were positively correlated with abundance of acanthocephalans. Carbon dioxide levels were negatively correlated with parasite diversity and monogenean abundance. Chloride levels were negatively correlated with parasite diversity. Dissolved oxygen levels were positively correlated with parasite diversity and monogenean abundance. Turbidity was positively correlated with parasite abundance, acanthocephalan abundance, and digenean abundance, and negatively correlated with parasite diversity. Parasite abundance was significantly lower in west
Texas and western river basins, and lower in rivers than ponds. Acanthocephalan abundance was significantly lower in rivers than ponds. Leech abundance was highest in the Trinity river basin. Turbidity had the strongest correlations in this study. As water clarity increased, diversity increased and abundance of certain taxa decreased, indicating clearer water may have greater food web diversity and healthier hosts. This study adds valuable data on host-parasite associations, parasite distributions, and parasite ecology of turtles in the state of
Texas. Many of these findings are likely transferable to other host taxa and should be studied in greater depth. Parasite diversity is not well known, even in common species, highlighting the need for more diversity surveys.
Advisors/Committee Members: Dronen, Norman O (advisor), Hibbitts, Toby J (committee member), Craig, Thomas M (committee member).
Subjects/Keywords: Parasite; Helminth; Ecology; Freshwater; Turtles; Texas; Acanthocephala; Trematoda; Nematoda; Monogenea; Hirudinea; Pentastomida
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APA (6th Edition):
Neely, W. J. (2018). A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173743
Chicago Manual of Style (16th Edition):
Neely, Wesley James. “A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles.” 2018. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/173743.
MLA Handbook (7th Edition):
Neely, Wesley James. “A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles.” 2018. Web. 18 Jan 2021.
Vancouver:
Neely WJ. A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles. [Internet] [Masters thesis]. Texas A&M University; 2018. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/173743.
Council of Science Editors:
Neely WJ. A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles. [Masters Thesis]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173743
Texas A&M University
3.
Luksovsky, Joe.
Combination Anthelmintics to Control Gastrointestinal Neamatodes in Foals.
Degree: MS, Biomedical Sciences, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10414
► A study was undertaken to evaluate and compare the effectiveness of three anthelmintics, ivermectin, fenbendazole, and a combination of ivermectin and pyrantel pamoate, on fecal…
(more)
▼ A study was undertaken to evaluate and compare the effectiveness of three anthelmintics, ivermectin, fenbendazole, and a combination of ivermectin and pyrantel pamoate, on fecal egg count reductions of cyathostomes and Parascaris equorum in 30 foals at the
Texas A&
M Horse Center. The foals were reared under standard horse center practices and were naturally infected with both cyathostomes and Parascaris. The foals were randomized into three treatment groups with individuals being rerandomized after each eight week observation period. The treatments of ivermectin and fenbendazole were given at the manufacturer's recommended doses and the pyrantel treatment was given at two times the manufacturer's recommended dose. All doses were based on weights taken prior to treatment. Fecal egg counts were performed at the time of treatment and at two week intervals after treatment for a total of eight weeks. Each foal received a total of three treatments during the course of the study along with the most effective treatment at the conclusion of the study. Fecal egg counts were performed by a modified McMaster's test with a sensitivity of 25 eggs per gram of feces and by the modified Wisconsin double centrifugal floatation with a sensitivity of 0.2 eggs per gram of feces. Fecal egg reduction percentages were calculated for each two week interval. Analysis of the results showed that ivermectin, either used alone or with pyrantel was a more effective anthelmintic for cyathostome (small strongyle) control than fenbendazole. Fenbendazole and pyrantel showed a higher initial reduction in Parascasris eggs when compared to the ivermectin only treated group, but ivermectin showed improved egg reduction over time. At the conclusion of this study, a primary treatment of ivermectin at the manufacturer's recommended dose and treatment of pyrantel at two times the manufacturer's recommended dose was recommended to control cyathostome egg production and severely reduce the initial number of Parascaris adults in the foals at this facility. Subsequent monthly does of ivermectin at the manufacturer's recommended dose was also recommended to continue to control both parasites. Follow up fecal examinations were also recommended to test the continued effectiveness of the recommended treatment protocol.
Advisors/Committee Members: Craig, Thomas M. (advisor), Cyr, Tracy (committee member), Forrest, David (committee member).
Subjects/Keywords: Cyathostomes; Parascaris; Anthelminthic resistance; Equine parasites; Fecal egg counts
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APA (6th Edition):
Luksovsky, J. (2012). Combination Anthelmintics to Control Gastrointestinal Neamatodes in Foals. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10414
Chicago Manual of Style (16th Edition):
Luksovsky, Joe. “Combination Anthelmintics to Control Gastrointestinal Neamatodes in Foals.” 2012. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10414.
MLA Handbook (7th Edition):
Luksovsky, Joe. “Combination Anthelmintics to Control Gastrointestinal Neamatodes in Foals.” 2012. Web. 18 Jan 2021.
Vancouver:
Luksovsky J. Combination Anthelmintics to Control Gastrointestinal Neamatodes in Foals. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10414.
Council of Science Editors:
Luksovsky J. Combination Anthelmintics to Control Gastrointestinal Neamatodes in Foals. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10414
Texas A&M University
4.
Ngere, Lauretta.
Estimation of Genetic Parameters and Assessment of Genetic Variation for Internal Parasite Resistance Traits in Ruminants.
Degree: PhD, Animal Breeding, 2015, Texas A&M University
URL: http://hdl.handle.net/1969.1/155097
► Internal parasites are a major concern to the livestock industry leading to huge losses. Genetic enhancement of ruminants for resistance/tolerance to internal parasites may provide…
(more)
▼ Internal parasites are a major concern to the livestock industry leading to huge losses. Genetic enhancement of ruminants for resistance/tolerance to internal parasites may provide for a lasting solution to the problem of internal parasite infection in livestock. The objective of this study was to estimate heritability and permanent environmental variance for internal parasite resistance traits in sheep and to apply penalties on the records of treated animals, analyzing the effect of such penalties on the genetic parameters. Records from 1008 Dorper sheep in a private South African flock comprised 17,711 FAMACHA scores, 3,758 fecal egg counts (mostly Haemonchus contortus), and 4,209 hematocrit values that were collected from 1997 – 2000. Animal models were used to conduct single trait analyses. Data were analyzed in three sets: 1) untreated records only; 2) all records; no penalties; and 3) all records with treated records penalized. Heritability estimates of Fc (FAMACHA) ranged from 0.33 ± 0.03 to 0.37 ± 0.03; FEC (Fecal egg count) from 0.04 ± 0.02 to 0.05 ± 0.03 and hematocrit from 0.19 ± 0.04 to 0.20 ± 0.05. Permanent environmental variance as a proportion of phenotypic variance was 0.02 ± 0.02 to 0.03 ± 0.02 for Fc, 0.14 ± 0.04 to 0.18 ± 0.05 for Ht and 0.07 ± 0.02 to 0.08 ± 0.03 for FEC. The Inclusion of treated animal records in the analyses, with or without penalization did not change the estimates of heritability and permanent environmental variance as a proportion of phenotypic variance.
The objective of the second study was to assess genetic variation in fecal egg count and the associations of fecal egg count with other traits in growing crossbred Nelore-Angus cattle. Records of 201 F2 and F3 ½ Nelore ½ Angus steers in feedlot conditions in a genomics resource population in Central
Texas were collected in 2012 and 2013. Helminth egg counts were determined from fecal samples before treatment with an anthelmintic product. The association of fecal egg count with other traits was assessed by modeling each in distinct analyses as a linear covariate. Year explained substantial variation in fecal egg count (P = 0.001). No other investigated covariate (birth weight, weaning weight, weaning temperament score, live weight, temperature, and exit velocity) was important in the different models (P > 0.2). Subsequently, sire (n= 13) was evaluated as a fixed effect (sires with less than 3 steers with records were excluded). Two sire families had significantly lower (P < 0.05) fecal egg counts (1.31 ± 0.28 and 1.57 ± 0.10) than the three sire families with the highest fecal egg counts (1.87 ± 0.10 - 2.06 ± 0.20). These results suggest the presence of additive genetic variation for fecal egg count, implying that selection can be carried out for the ability to suppress parasite worms in cattle.
The objective of the second study was to assess genetic variation in fecal egg count and the associations of fecal egg count with other traits in growing crossbred Nelore-Angus cattle. Records of 201 F2 and F3 ½ Nelore ½ Angus…
Advisors/Committee Members: Riley, David (advisor), Sanders, James (committee member), Herring, Andy (committee member), Craig, Thomas (committee member).
Subjects/Keywords: Internal Parasite; FAMACHA; Heritability; Penalization
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APA (6th Edition):
Ngere, L. (2015). Estimation of Genetic Parameters and Assessment of Genetic Variation for Internal Parasite Resistance Traits in Ruminants. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/155097
Chicago Manual of Style (16th Edition):
Ngere, Lauretta. “Estimation of Genetic Parameters and Assessment of Genetic Variation for Internal Parasite Resistance Traits in Ruminants.” 2015. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/155097.
MLA Handbook (7th Edition):
Ngere, Lauretta. “Estimation of Genetic Parameters and Assessment of Genetic Variation for Internal Parasite Resistance Traits in Ruminants.” 2015. Web. 18 Jan 2021.
Vancouver:
Ngere L. Estimation of Genetic Parameters and Assessment of Genetic Variation for Internal Parasite Resistance Traits in Ruminants. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/155097.
Council of Science Editors:
Ngere L. Estimation of Genetic Parameters and Assessment of Genetic Variation for Internal Parasite Resistance Traits in Ruminants. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/155097
Texas A&M University
5.
Carroll, Juliette E.
Babesia microti Recombinant DNA Vaccine as a Model for Babesia bovis Prevention.
Degree: MS, Veterinary Parasitology, 2011, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7248
► Babesiosis is caused by a genus of tick-transmitted apicomplexan parasites with considerable economic, medical, and veterinary impact. Bovine babesiosis is an important impediment to livestock…
(more)
▼ Babesiosis is caused by a genus of tick-transmitted apicomplexan parasites with considerable economic, medical, and veterinary impact. Bovine babesiosis is an important impediment to livestock production throughout the world. Limited options are available for control of this widespread protozoal disease. This study evaluated the protective effect of DNA vaccines incorporating Babesia cysteine proteases and Apical Membrane Antigen-1 separately and in combination. The Helios Gene Gun System was used to vaccinate BALB/c mice with plasmid DNA constructs encoding different B. microti proteins (pBmCP1, pBmAMA1 or a combination of pBmCP1 and pBmAMA1). An analysis of the parasitemia post-challenge supports the hypothesis that pBmCP1 and pBmAMA1 induce protective effects against the progression of the parasite. However, the combination of the two constructs given simultaneously has no marked effect on parasite progression. Furthermore, the data obtained from the packed cell volumes of the mice indicates that only BmCP1 is able to reduce this effect of clinical disease with any level of significance. Babesia bovis constructs containing Cysteine Protease-2 and Apical Membrane Antigen-1 were created and sequence verified for use in future vaccination studies. The results seen using the mouse model of Babesiosis may provide applicable information for the design of vaccines against other Babesia spp., particularly for B. bovis.
Advisors/Committee Members: Holman, Patricia (advisor), Craig, Thomas (committee member), Russell, Leon (committee member), Mwangi, Waithaka (committee member).
Subjects/Keywords: Babesia spp. vaccine
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APA (6th Edition):
Carroll, J. E. (2011). Babesia microti Recombinant DNA Vaccine as a Model for Babesia bovis Prevention. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7248
Chicago Manual of Style (16th Edition):
Carroll, Juliette E. “Babesia microti Recombinant DNA Vaccine as a Model for Babesia bovis Prevention.” 2011. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7248.
MLA Handbook (7th Edition):
Carroll, Juliette E. “Babesia microti Recombinant DNA Vaccine as a Model for Babesia bovis Prevention.” 2011. Web. 18 Jan 2021.
Vancouver:
Carroll JE. Babesia microti Recombinant DNA Vaccine as a Model for Babesia bovis Prevention. [Internet] [Masters thesis]. Texas A&M University; 2011. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7248.
Council of Science Editors:
Carroll JE. Babesia microti Recombinant DNA Vaccine as a Model for Babesia bovis Prevention. [Masters Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7248
Texas A&M University
6.
Neely, Wesley James.
A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles.
Degree: MS, Wildlife and Fisheries Sciences, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/173838
► In this study, the metazoan parasites of three species of freshwater turtles (the spiny softshell, Apalone spinifera, the common snapping turtle, Chelydra serpentina, and the…
(more)
▼ In this study, the metazoan parasites of three species of freshwater turtles (the spiny softshell, Apalone spinifera, the common snapping turtle, Chelydra serpentina, and the red-eared slider, Trachemys scripta elegans) were surveyed at 16 sites across the state of
Texas. A total of 42 species of metazoan parasites were recovered from 15 A. spinifera, nine C. serpentina, and 55 T. s. elegans, representing 16 new host-parasite associations and 17 new locality records. The synonymy of Acanthostomum nuevoleonensis by Brooks (1980) is refuted and the species is redescribed.
Two new species of monogenean worms in the genus Neopolystoma are reported, one from C. serpentina and A. spinifera and another from T. s. elegans. Through non-metric multidimensional scaling and analysis of similarities, A. spinifera was found to contain a significantly distinct parasite community from C. serpentina and T. s. elegans. A range of water parameters (ammonia, carbon dioxide, chloride, dissolved oxygen, hardness, nitrite, nitrate, pH, salinity, temperature, and turbidity) were recorded on each sampling trip and compared to parasite abundance and diversity. Ammonia levels were positively correlated with abundance of acanthocephalans. Carbon dioxide levels were negatively correlated with parasite diversity and monogenean abundance. Chloride levels were negatively correlated with parasite diversity. Dissolved oxygen levels were positively correlated with parasite diversity and monogenean abundance. Turbidity was positively correlated with parasite abundance, acanthocephalan abundance, and digenean abundance, and negatively correlated with parasite diversity. Parasite abundance was significantly lower in west
Texas and western river basins, and lower in rivers than ponds. Acanthocephalan abundance was significantly lower in rivers than ponds. Leech abundance was highest in the Trinity river basin. Turbidity had the strongest correlations in this study. As water clarity increased, diversity increased and abundance of certain taxa decreased, indicating clearer water may have greater food web diversity and healthier hosts. This study adds valuable data on host-parasite associations, parasite distributions, and parasite ecology of turtles in the state of
Texas. Many of these findings are likely transferable to other host taxa and should be studied in greater depth. Parasite diversity is not well known, even in common species, highlighting the need for more diversity surveys.
Advisors/Committee Members: Dronen, Norman O (advisor), Hibbitts, Toby J (committee member), Craig, Thomas M (committee member).
Subjects/Keywords: Parasite; Helminth; Ecology; Freshwater; Turtles; Texas; Acanthocephala; Trematoda; Nematoda; Monogenea; Hirudinea; Pentastomida
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APA ·
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CSE |
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Manager
APA (6th Edition):
Neely, W. J. (2018). A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173838
Chicago Manual of Style (16th Edition):
Neely, Wesley James. “A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles.” 2018. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/173838.
MLA Handbook (7th Edition):
Neely, Wesley James. “A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles.” 2018. Web. 18 Jan 2021.
Vancouver:
Neely WJ. A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles. [Internet] [Masters thesis]. Texas A&M University; 2018. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/173838.
Council of Science Editors:
Neely WJ. A Study on the Parasite Diversity and Community Ecology of Three Species of Texas Freshwater Turtles. [Masters Thesis]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173838
Texas A&M University
7.
Bingham, Glenda.
The Epidemiology and Impact of Dog-Associated Zoonoses in Developed and Developing Countries.
Degree: PhD, Biomedical Sciences, 2014, Texas A&M University
URL: http://hdl.handle.net/1969.1/153674
► Dogs are an important part of human societies throughout the world. Although people derive many economic and health benefits from their relationships with dogs, these…
(more)
▼ Dogs are an important part of human societies throughout the world. Although people derive many economic and health benefits from their relationships with dogs, these relationships are not without risk. Interaction with dogs can lead to an increased risk for zoonotic diseases, if the appropriate precautions are not taken. While dogs contribute to the transmission of a variety of zoonoses, only a
few of these diseases, such as rabies and cystic echinococcosis, are recognized as having widespread economic and public health implications.
In order to assess the knowledge and perceptions of dog-associated zoonoses in Brazos County,
Texas, random digit dialing was used to select households for participation in a cross-sectional telephone survey. Of the 1691 households, which were contacted, 922 (55%) completed the interview. Dog owners accounted for 56% of the study participants. Eighty-six percent of the
respondents indicated they would report being bitten by a dog that they did not own. Factors that were shown to be significantly associated with bite reporting included, age, sex, urbanicity, and rabies knowledge. Many of the respondents lacked knowledge about dog-associated zoonoses, which could seriously affect their health.
Cystic echinococcosis (CE) is known to be endemic in Rio Negro Province, Argentina. However, current epidemiological data were not available for this region. A community-based CE screening study, which incorporated diagnostic imaging and a questionnaire, was conducted to evaluate the prevalence of human CE and identify locally relevant risk factors in the study population. The overall CE prevalence among the community volunteers was 7.1%, with 1.6% of children, and 10% of adults diagnosed as CE-positive. Age, level of education, dog ownership, and contact with sheep were found to be significantly associated with CE status.
In regions of the world where CE is highly endemic, it has been shown to be a substantial financial burden for those who are affected. When the costs associated with human CE, obtained from hospital chart reviews and patient interviews, were combined with livestock-associated costs obtained from government reports and scientific publications, the estimated total annual cost of CE in Rio Negro Province was 5,100,373 (95% CI: 2,852,345–8,004,122).
Advisors/Committee Members: Budke, Christine (advisor), Slater, Margaret R. (committee member), Norby, Bo (committee member), Craig, Thomas M. (committee member).
Subjects/Keywords: Zoonoses; Dogs
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APA (6th Edition):
Bingham, G. (2014). The Epidemiology and Impact of Dog-Associated Zoonoses in Developed and Developing Countries. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153674
Chicago Manual of Style (16th Edition):
Bingham, Glenda. “The Epidemiology and Impact of Dog-Associated Zoonoses in Developed and Developing Countries.” 2014. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/153674.
MLA Handbook (7th Edition):
Bingham, Glenda. “The Epidemiology and Impact of Dog-Associated Zoonoses in Developed and Developing Countries.” 2014. Web. 18 Jan 2021.
Vancouver:
Bingham G. The Epidemiology and Impact of Dog-Associated Zoonoses in Developed and Developing Countries. [Internet] [Doctoral dissertation]. Texas A&M University; 2014. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/153674.
Council of Science Editors:
Bingham G. The Epidemiology and Impact of Dog-Associated Zoonoses in Developed and Developing Countries. [Doctoral Dissertation]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153674
Texas A&M University
8.
Schuster, Anthony.
Spatial and Temporal Survey of Feral Pig Ectoparasites in Three Texas Wildlife Districts.
Degree: PhD, Entomology, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10290
► Feral pigs, European wild boars and their crosses are ubiquitous and found in all ecological zones from Florida to California. These introduced animals are recorded…
(more)
▼ Feral pigs, European wild boars and their crosses are ubiquitous and found in all ecological zones from Florida to California. These introduced animals are recorded in 39 US states and four Canadian provinces.
Texas currently has an estimated population of 1-4 million pigs with the potential to exceed 4 million based on suitable habitat estimates. Feral pigs can modify local flora and fauna and cause significant physical damage with their rooting activities. They can also reintroduce parasites and pathogens to previously parasite and pathogen free herds of domestic cattle, horses, sheep, and goats. The two overarching objectives of this research were to determine what role feral pigs have in the maintenance and possible distribution of fleas, lice, and ticks common to the three wildlife districts; and if they serve as bridging hosts for the same (or other) arthropods and their natural hosts. The supporting objectives were to establish host records of fleas, lice, and ticks parasitizing feral pigs; determine species assemblies within each of the three wildlife districts; and to compare species assemblies among the wildlife districts.
Feral pigs (564) were taken from June 2008 to March 2011 using box, corral, and panel traps in three wildlife districts. Two hundred fifty six fleas, Pulex porcinus (Jordan and Rothschild), were collected from all gender and age classes of feral pigs at the South
Texas Plains wildlife district. No fleas were collected at either the Hill Country or Post Oak Savannah wildlife districts. This is the first report of these fleas on feral pigs. Lice and ticks were collected from all gender and age classes of feral pigs from all sample sites. Only hog lice, Haematopinus suis, were collected at all three sample sites. Seven species of ticks were collected from the three sites: Amblyomma americanum, A. cajennense, A. maculatum, Dermacentor albipictus, D. halli, D. variabilis, and Ixodes scapularis. Amblyomma cajennense was collected only at the South
Texas Plains sample site; A. americanum and I. scapularis were collected only at the Hill Country and Post Oak sample sites. This study reports that feral pigs are serving as hosts for one species of flea, one species of lice and seven species of ticks common to
Texas.
Advisors/Committee Members: Teel, Pete D. (advisor), Mulenga, Albert (committee member), Craig, Thomas M. (committee member), Russell, Leon H. (committee member), Sames III, William J. (committee member).
Subjects/Keywords: Feral pig; ectoparasites; javelina flea; Key Phrase Two; hog lice; ticks; Welder Foundation
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APA (6th Edition):
Schuster, A. (2012). Spatial and Temporal Survey of Feral Pig Ectoparasites in Three Texas Wildlife Districts. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10290
Chicago Manual of Style (16th Edition):
Schuster, Anthony. “Spatial and Temporal Survey of Feral Pig Ectoparasites in Three Texas Wildlife Districts.” 2012. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10290.
MLA Handbook (7th Edition):
Schuster, Anthony. “Spatial and Temporal Survey of Feral Pig Ectoparasites in Three Texas Wildlife Districts.” 2012. Web. 18 Jan 2021.
Vancouver:
Schuster A. Spatial and Temporal Survey of Feral Pig Ectoparasites in Three Texas Wildlife Districts. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10290.
Council of Science Editors:
Schuster A. Spatial and Temporal Survey of Feral Pig Ectoparasites in Three Texas Wildlife Districts. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10290
9.
Arenaz, Cristina M.
Determination of Life Cycle Stages of Body Cavity Fluke Cyclocoelids (Cyclocoelidae) in Terrestrial Snails and Experimental Exposure of Domestic Chickens (Gallus gallus).
Degree: MS, Wildlife and Fisheries Sciences, 2015, Texas A&M University
URL: http://hdl.handle.net/1969.1/156469
► Zoos and other bird-holding facilities world-wide recently have reported serious health problems and frequently deaths of captive birds in exhibits associated with infections by introduced…
(more)
▼ Zoos and other bird-holding facilities world-wide recently have reported serious health problems and frequently deaths of captive birds in exhibits associated with infections by introduced species of cyclocoel (Digenea) parasites. These adult flukes apparently have been introduced into these facilities by the importation of infected exotic birds. The larval stages of digenean species generally develop within either aquatic or terrestrial snails. A few of the life cycles of cyclocoelids that utilize aquatic snails as the molluscan host have been documented; however, very little is known about the life cycle of the species introduced into bird-holding facilities where the larval stages are produced in terrestrial snails. A more complete understanding of these terrestrial-based life cycles is essential for the effective prevention and treatment of infected birds. Ultimately, our objective is the development of practical control strategies for these parasites in zoos.
In this study, 16 of 53 terrestrial snails (30%) of the genus Subulina from Lincoln
Park Zoo, Chicago, Illinois were found to be infected with cyclocoelid parasites of the genus Szidatitrema. Larval stages (redial generations, cercariae, metacercariae, and young adults) of the parasite taken from these snails were described and illustrated. Metacercariae were suspended in a saline solution administered to 15 one-day old chickens. Five control chickens were given saline solution. Fecal samples were taken weekly from experimental and control chickens to determine if eggs were present through sedimentation and floatation procedures. Blood samples were obtained from chickens every other week starting in the second week post-exposure. A total of eight chickens were necropsied starting on Day 67.
Experimental infections were not successful; there were no differences between levels of eosinophils or fibrinogen in experimental chicks compared to control chicks, and helminth eggs were not found in any fecal floatation. No adult flukes were found during necropsy. Further studies should include attempts to experimentally infect additional bird hosts including monk parakeets from the Schubot breeding colony at
Texas A&
M University, testing of different exposure methods, and determination of any potential secondary intermediate hosts or paratenic hosts. Once experimental bird hosts have been successfully infected in a laboratory setting, vaccines, drug trials, and potential detection methods for this fluke should be considered.
Advisors/Committee Members: Dronen, Norman (advisor), Tizard, Ian (advisor), Craig, Thomas (committee member).
Subjects/Keywords: cyclocoelid; Cyclocoelidae; Digenea; Szidatitrema; flukes; life cycle; metacercaria; chickens; terrestrial snails; Subulina; air sacs; exotic birds; zoos; parasites; parasitology
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APA (6th Edition):
Arenaz, C. M. (2015). Determination of Life Cycle Stages of Body Cavity Fluke Cyclocoelids (Cyclocoelidae) in Terrestrial Snails and Experimental Exposure of Domestic Chickens (Gallus gallus). (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156469
Chicago Manual of Style (16th Edition):
Arenaz, Cristina M. “Determination of Life Cycle Stages of Body Cavity Fluke Cyclocoelids (Cyclocoelidae) in Terrestrial Snails and Experimental Exposure of Domestic Chickens (Gallus gallus).” 2015. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/156469.
MLA Handbook (7th Edition):
Arenaz, Cristina M. “Determination of Life Cycle Stages of Body Cavity Fluke Cyclocoelids (Cyclocoelidae) in Terrestrial Snails and Experimental Exposure of Domestic Chickens (Gallus gallus).” 2015. Web. 18 Jan 2021.
Vancouver:
Arenaz CM. Determination of Life Cycle Stages of Body Cavity Fluke Cyclocoelids (Cyclocoelidae) in Terrestrial Snails and Experimental Exposure of Domestic Chickens (Gallus gallus). [Internet] [Masters thesis]. Texas A&M University; 2015. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/156469.
Council of Science Editors:
Arenaz CM. Determination of Life Cycle Stages of Body Cavity Fluke Cyclocoelids (Cyclocoelidae) in Terrestrial Snails and Experimental Exposure of Domestic Chickens (Gallus gallus). [Masters Thesis]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156469
10.
McKown, Richard Dwayne.
Localization and partial immunological characterization of Fasciola hepatica Thioredoxin.
Degree: PhD, Veterinary Microbiology, 2005, Texas A&M University
URL: http://hdl.handle.net/1969.1/1401
► This study reports the localization and partial characterization of thioredoxin from the parasitic trematode Fasciola hepatica. Snails (Pseudosuccinia columella) were raised in culture and infected…
(more)
▼ This study reports the localization and partial characterization of thioredoxin from the parasitic trematode Fasciola hepatica. Snails (Pseudosuccinia columella) were raised in culture and infected with F. hepatica so that Western blotting and immunohistochemical techniques could be utilized to determine the presence of thioredoxin in different stages of the parasites development. The results of these experiments showed that thioredoxin was present in the tegument, gut epithelium, excretory canal epithelium and sperm, of the adult parasite as well as in the tegument and gut of the redia and cercaria intermediate stages. In situ hybridization was used to determine the localization and possible differential mRNA expression of two different F. hepatica thioredoxin isotypes (Fh2020.A and Fh2020.SL) in the adult parasite. The in situ hybridization results showed that both isotypes are expressed in the tegument and gut epithelium. Fh2020.A stains with a greater intensity possibly demonstrating a difference in the amount of expression between the two isotypes.
Recombinant F. hepatica thioredoxin expressed in bacteria using the pMAL Protein Fusion and Expression System was used to test its affects on the production of super oxide anion by murine peritoneal macrophages, bovine monocyte-derived macrophages and bovine whole blood neutrophils, and nitric oxide production by mouse peritoneal macrophages and bovine monocyte-derived macrophages. The results of the cellular assays were not definitive due to the fact that the maltose binding protein (MBP) moiety of the recombinant thioredoxin, when tested alone, increased production of nitric oxide by bovine monocyte-derived macrophages. Consequently, since the MBP could not be effectively separated from the thioredoxin portion of the recombinant, allowing the thioredoxin affects to be tested independently, no true conclusions regarding its affects on the host immune cells tested could be drawn.
This is the first report of the localization of thioredoxin in both the adult F. hepatica as well as in specific intermediate stages of the parasite. These studies demonstrate the possible affects that a protein tag can have on experimental results and demonstrate how such data may be interpreted when a non-cleaved recombinant protein is used in cellular or other assays when compared to native or cleaved recombinant proteins.
Advisors/Committee Members: Rice-Ficht, Allison (advisor), Craig, Thomas (advisor), Snowden, Karen (committee member), Teel, Pete (committee member).
Subjects/Keywords: thioredoxin; Fasciola hepatica; liver fluke; immunohistochemistry; in situ hybridization; nitric oxide; superoxide
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APA (6th Edition):
McKown, R. D. (2005). Localization and partial immunological characterization of Fasciola hepatica Thioredoxin. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/1401
Chicago Manual of Style (16th Edition):
McKown, Richard Dwayne. “Localization and partial immunological characterization of Fasciola hepatica Thioredoxin.” 2005. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/1401.
MLA Handbook (7th Edition):
McKown, Richard Dwayne. “Localization and partial immunological characterization of Fasciola hepatica Thioredoxin.” 2005. Web. 18 Jan 2021.
Vancouver:
McKown RD. Localization and partial immunological characterization of Fasciola hepatica Thioredoxin. [Internet] [Doctoral dissertation]. Texas A&M University; 2005. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/1401.
Council of Science Editors:
McKown RD. Localization and partial immunological characterization of Fasciola hepatica Thioredoxin. [Doctoral Dissertation]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/1401
Texas A&M University
11.
Garretson, Pamela Donn.
Role of P-glycoprotein in Haemonchus contortus anthelmintic resistance.
Degree: MS, Veterinary Parasitology, 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-1633
► The gastrointestinal parasite, Haemonchus contortus, is of major concern in the sheep and goat industry as well as in zoological settings. Over the years this…
(more)
▼ The gastrointestinal parasite, Haemonchus contortus, is of major concern in the
sheep and goat industry as well as in zoological settings. Over the years this parasite has
developed resistance to the three classes of anthelmintics, benzimidazoles,
imidazothiazoles and macrocyclic lactones, that are currently used for treatment. One of
the mechanisms proposed to be involved in this resistance is the efflux transporter
P-glycoprotein (Pgp). In this study, the resistance status of several strains of
H. contortus was evaluated using the larval development assay DrenchRite®. After
documenting the resistance status of these strains, transcription of Pgp in L3 larvae after
exposure to anthelmintics was quantitated using polymerase chain reaction (PCR). Of
the strains analyzed, only one was determined to be susceptible to all of the
anthelmintics tested, while the others showed variable levels of resistance to one or
more. A Haemonchus strain acquired from a giraffe at a zoo in Florida was the most
resistant, showing extremely high levels of resistance to benzimidazoles and levamisole.
Molecular characterization of the 18S rRNA gene and the internal transcriber spacer
region (ITS) were performed on the giraffe strain to identify the species. Although there
were variations in the isolate sequences, the most likely species for the giraffe strain was
H. contortus. No transcription of Pgp was identified in H. contortus L3 larvae under the conditions of this study. Thus, increased Pgp does not appear to be a primary
mechanism of drug resistance in this stage of the worm.
Advisors/Committee Members: Holman, Patricia (advisor), Craig, Thomas (committee member), Gill, Clare (committee member).
Subjects/Keywords: Anthelmintic resistance; Goats; Haemonchus contortus; Haemonchosis; P-glycoprotein; Sheep
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APA (6th Edition):
Garretson, P. D. (2009). Role of P-glycoprotein in Haemonchus contortus anthelmintic resistance. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1633
Chicago Manual of Style (16th Edition):
Garretson, Pamela Donn. “Role of P-glycoprotein in Haemonchus contortus anthelmintic resistance.” 2009. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-1633.
MLA Handbook (7th Edition):
Garretson, Pamela Donn. “Role of P-glycoprotein in Haemonchus contortus anthelmintic resistance.” 2009. Web. 18 Jan 2021.
Vancouver:
Garretson PD. Role of P-glycoprotein in Haemonchus contortus anthelmintic resistance. [Internet] [Masters thesis]. Texas A&M University; 2009. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1633.
Council of Science Editors:
Garretson PD. Role of P-glycoprotein in Haemonchus contortus anthelmintic resistance. [Masters Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1633
Texas A&M University
12.
Lehtinen, Lauren Elyse.
Canine babesiasis: occurrence and molecular characterization of Babesia isolates.
Degree: MS, Veterinary Parasitology, 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-1634
► Canine babesiosis is an important worldwide disease caused by protozoan hemoparasites of the genus Babesia, which are primarily transmitted to a dog by the bite…
(more)
▼ Canine babesiosis is an important worldwide disease caused by protozoan
hemoparasites of the genus Babesia, which are primarily transmitted to a dog by the bite
of an Ixodid tick, although vertical transmission has recently been reported. The disease
is typically characterized by hemolytic anemia, fever, splenomegaly, and
thrombocytopenia, with clinical signs ranging from clinically normal to acute anemia.
Death may even result in some severe cases. Two species of Babesia, Babesia gibsoni
and Babesia canis, have long been known to cause babesiosis in dogs. To date, almost
all B. gibsoni infections in the United States have been reported in American Pit Bull
Terriers or in dogs associated with the breed through either transfusion or fighting.
Dog blood samples received from kennels, shelters, and veterinary clinics
throughout
Texas were tested for the presence of B. gibsoni and B. canis. A total of 254
samples were tested for B. gibsoni and B. canis by light microscopy and polymerase
chain reaction (PCR). Babesia gibsoni was detected in four of the dogs tested and B.
canis was detected in one of the dogs tested. The average packed cell volumes (PCVs)
of infected dogs were compared with those of uninfected dogs, with the infected, on
average, having lower PCVs. Molecular characterization of the small subunit ribosomal RNA gene and the ribosomal RNA internal transcribed spacer regions was performed on
all sequences obtained in this study, and results were consistent with those previously
reported for B. gibsoni and B. canis. Also, positive samples and additional samples
provided by North Carolinia State
University were used to initiate in vitro cultures of the
parasites. To date, one isolate of a large unknown Babesia sp. from a North Carolina
dog was successfully established in vitro. The establishment of Babesia spp. parasites in
culture may aid in the development of a vaccine for babesiosis and will also be
beneficial in improving diagnostic tests for the parasite.
Advisors/Committee Members: Holman, Patricia (advisor), Budke, Christine (committee member), Craig, Thomas M. (committee member).
Subjects/Keywords: Babeisa canis; Babesia gibsoni; Canine babesiasis; Polymerase chain reaction; Parasite Cultures; Ribosomal RNA
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APA (6th Edition):
Lehtinen, L. E. (2009). Canine babesiasis: occurrence and molecular characterization of Babesia isolates. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1634
Chicago Manual of Style (16th Edition):
Lehtinen, Lauren Elyse. “Canine babesiasis: occurrence and molecular characterization of Babesia isolates.” 2009. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-1634.
MLA Handbook (7th Edition):
Lehtinen, Lauren Elyse. “Canine babesiasis: occurrence and molecular characterization of Babesia isolates.” 2009. Web. 18 Jan 2021.
Vancouver:
Lehtinen LE. Canine babesiasis: occurrence and molecular characterization of Babesia isolates. [Internet] [Masters thesis]. Texas A&M University; 2009. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1634.
Council of Science Editors:
Lehtinen LE. Canine babesiasis: occurrence and molecular characterization of Babesia isolates. [Masters Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1634
Texas A&M University
13.
Bendele, Kylie Gayle.
Molecular characterization of Theileria spp. using ribosomal RNA.
Degree: MS, Veterinary Parasitology, 2005, Texas A&M University
URL: http://hdl.handle.net/1969.1/2649
► The molecular characterization of twenty six Theileria spp. isolates and one C. felis isolate were done on the small subunit ribosomal RNA (SSU rRNA) gene,…
(more)
▼ The molecular characterization of twenty six Theileria spp. isolates and one C. felis isolate were done on the small subunit ribosomal RNA (SSU rRNA) gene, the 5.8S gene, and the two internal transcribed spacer regions using gDNA. The SSU rRNA gene is increasingly accepted as a widely used marker for characterization, taxonomic classification, and phylogenetic analysis and this gene has been sequenced from a variety of different organisms, resulting in a large database for sequence comparisons (Chae et al. 1998; Chae et al., 1999 a,b,c; Stockham et al., 2000; Cossio-Bayugar et al., 2002; Gubbels et al., 2000). The genomic region consists of the internal transcribed spacer 1 (ITS 1), the 5.8S gene, and internal transcribed spacer 2 (ITS 2) (ITS 1-5.8S-ITS 2 gene region) and separates the SSU rRNA gene from the large subunit ribosomal RNA gene. The 5.8S rRNA gene is highly conserved in size and nucleotide sequence, is relatively constant in molecular weight, and has an average chain length of approximately 160 nucleotides and has proven useful in dividing subgenera of Gyrodactylus ((Nazar, 1984; Zietara et al., 2002).
Pairwise comparisons were done between the clones of an individual isolate and among the clones of the different isolates. Phylogenetic trees were made from the resulting sequences. This study shows that different SSU rRNA genes may be associated with ITS 1-5.8S-ITS 2 gene regions of distinct sequence in the same isolate. This study also demonstrates that considerable ITS 1-5.8S-ITS 2 gene region sequence variation may exist within a species. This may be useful for subspeciation designation, or may simply reflect considerable variation within the population. This study shows that the ITS 1-5.8S-ITS 2 gene region may be a useful molecular marker for the taxonomy of Theileria spp.
Advisors/Committee Members: Holman, Patricia (advisor), Teel, Pete (committee member), Craig, Thomas (committee member).
Subjects/Keywords: Theileria; small subunit rRNA; ITS 1-5.8S-ITS 2 gene region
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APA (6th Edition):
Bendele, K. G. (2005). Molecular characterization of Theileria spp. using ribosomal RNA. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2649
Chicago Manual of Style (16th Edition):
Bendele, Kylie Gayle. “Molecular characterization of Theileria spp. using ribosomal RNA.” 2005. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/2649.
MLA Handbook (7th Edition):
Bendele, Kylie Gayle. “Molecular characterization of Theileria spp. using ribosomal RNA.” 2005. Web. 18 Jan 2021.
Vancouver:
Bendele KG. Molecular characterization of Theileria spp. using ribosomal RNA. [Internet] [Masters thesis]. Texas A&M University; 2005. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/2649.
Council of Science Editors:
Bendele KG. Molecular characterization of Theileria spp. using ribosomal RNA. [Masters Thesis]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2649
Texas A&M University
14.
Schoelkopf, Lorien.
Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.
Degree: MS, Veterinary Parasitology, 2005, Texas A&M University
URL: http://hdl.handle.net/1969.1/2660
► Babesia odocoilei is an intraerythrocytic apicomplexan parasite which infects cervidae, sometimes causing babesiosis. It is vectored by the tick Ixodes scapularis and is distributed throughout…
(more)
▼ Babesia odocoilei is an intraerythrocytic apicomplexan parasite which infects cervidae, sometimes causing babesiosis. It is vectored by the tick Ixodes scapularis and is distributed throughout the southeastern United States. The geographic and host range continue to extend as new incidence of infection is detected.
A genomic DNA region spanning the internal transcribed spacer 1 (ITS1), 5.8S rRNA gene, and ITS2 of ribosomal RNA (rRNA) from 18 B. odocoilei isolates (speciation confirmed by small subunit rRNA analysis) was amplified using the polymerase chain reaction, cloned and sequenced. The isolates originated from 6 different cervidae or bovidae hosts in various U.S. geographic areas. Included in the analysis was a previously described reindeer B. odocoilei-like isolate, RD61, which showed only 99.0% identity in SSU rRNA analysis to B. odocoilei. Percent identity pairwise comparisons among the samples were calculated for both the full ITS1-5.8S-ITS2 and individual genomic regions. Identity values for all comparisons ranged from 90% to 100%, with the exception of RD61, which showed no higher than 88% identity for all gene regions.
An analysis of fixed differences identified in the ITS1 and ITS2 gene regions of all clones revealed 21 fixed differences in ITS1, and only 11 in ITS2. Most isolates were found to have 2 overall patterns of fixed differences, although some had 1 or 3.
Phylogenetic analysis of all sequences for the entire ITS1-5.8S-ITS2 gene region placed most isolates into 2 distinct groups corresponding to those observed in the analysis of fixed differences. This suggested the presence of at least 2 rRNA transcription units in
B. odocoilei.
ITS analysis failed to demonstrate host or geographic differences that might serve to pinpoint the source of outbreaks of B. odocoilei in farmed and managed host animals. This failure might result from genetic recombination of ITS genomic regions during the tick vector stage. Lack of conspecificity between the RD61 isolate and B. odocoilei was supported by this study; however, more data are needed to clarify the taxonomic status of this B. odocoilei-like isolate.
Advisors/Committee Members: Holman, Patricia J. (advisor), Teel, Pete D. (committee member), Craig, Thomas M. (committee member).
Subjects/Keywords: Babesia odocoilei; Internal transcribed spacers (ITS); Ribosomal RNA
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Export
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APA (6th Edition):
Schoelkopf, L. (2005). Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2660
Chicago Manual of Style (16th Edition):
Schoelkopf, Lorien. “Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.” 2005. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/2660.
MLA Handbook (7th Edition):
Schoelkopf, Lorien. “Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA.” 2005. Web. 18 Jan 2021.
Vancouver:
Schoelkopf L. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. [Internet] [Masters thesis]. Texas A&M University; 2005. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/2660.
Council of Science Editors:
Schoelkopf L. Molecular comparisons of Babesia odocoilei using the internal transcribed spacers of ribosomal RNA. [Masters Thesis]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2660
Texas A&M University
15.
Paczkowski, Melissa Jeanne.
Effects of experimental fascioliasis on puberty and comparison of mounting activity by radiotelemetry in pubertal and gestating beef heifers.
Degree: MS, Physiology of Reproduction, 2005, Texas A&M University
URL: http://hdl.handle.net/1969.1/2796
► Angus-sired heifers were allotted by age (mean=4 mo), BW (mean=135 kg), and sire (n=4) to either a control (n=10) or infected group (n=11; 600 metacercariae…
(more)
▼ Angus-sired heifers were allotted by age (mean=4 mo), BW (mean=135 kg), and sire (n=4) to either a control (n=10) or infected group (n=11; 600 metacercariae of Fasciola hepatica, intraruminally) to test our hypothesis that puberty is delayed by experimental fascioliasis. Blood samples were collected biweekly for analysis of steroid hormone concentrations. At 2-wk intervals, BW was recorded, and samples were collected for analysis of liver enzymes and serum proteins and fecal egg counts. A radiotelemetry system (HeatWatch??) was used to detect estrus and ovulation was confirmed by an elevation in serum progesterone (P4) after estrus. Heifers were artificially inseminated (AI) at the second observed estrus. Serum γ-glutamyl transpeptidase (GGT) and aspartate aminotransferase (AST) increased (p<0.0008) between day 0 and 112 in the infected group. Serum estradiol (E2) and P4 concentrations did not differ (p>0.1) between treatment groups. Mean age at puberty was 10 days later (p>0.1) in the infected group. Conception rate did not differ between control and infected heifers.
The HeatWatch?? data were used to compare mounting activity during estrus in pubertal and gestating heifers. Mean duration of estrus was longer (p<0.01) for the second than for the pubertal estrus, though total mount duration and number of mounts did not differ. Number of mounts at second estrus was greater (p<0.05) for heifers that conceived (n=9). Mean duration of estrus and total mount duration at second estrus were not associated with pregnancy outcome. Estrus events were detected in all nine heifers during pregnancy (total=73). A majority (75%) of the interestrus intervals during gestation was <17 d. Number of mounts (p=0.035) and total duration of mounts (p=0.022) at second estrus were predictive of number of mounts during gestation.
Experimental infection of Fasciola hepatica did not alter serum steroid hormone concentration or delay pubertal development in heifers. Estrus duration was longer for the second estrus compared to the pubertal estrus, and the number of mounts received during the second estrus was greater in heifers that did conceive to AI. Estrus events were detected in each heifer during pregnancy; however, a normal interestrus interval occurred in only 10% of the estrus events.
Advisors/Committee Members: Forrest, David (advisor), Magee, Derry (committee member), Thompson, James (committee member), Craig, Thomas (committee member).
Subjects/Keywords: Fascioliasis; Puberty; Heifers; HeatWatch
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APA (6th Edition):
Paczkowski, M. J. (2005). Effects of experimental fascioliasis on puberty and comparison of mounting activity by radiotelemetry in pubertal and gestating beef heifers. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/2796
Chicago Manual of Style (16th Edition):
Paczkowski, Melissa Jeanne. “Effects of experimental fascioliasis on puberty and comparison of mounting activity by radiotelemetry in pubertal and gestating beef heifers.” 2005. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/2796.
MLA Handbook (7th Edition):
Paczkowski, Melissa Jeanne. “Effects of experimental fascioliasis on puberty and comparison of mounting activity by radiotelemetry in pubertal and gestating beef heifers.” 2005. Web. 18 Jan 2021.
Vancouver:
Paczkowski MJ. Effects of experimental fascioliasis on puberty and comparison of mounting activity by radiotelemetry in pubertal and gestating beef heifers. [Internet] [Masters thesis]. Texas A&M University; 2005. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/2796.
Council of Science Editors:
Paczkowski MJ. Effects of experimental fascioliasis on puberty and comparison of mounting activity by radiotelemetry in pubertal and gestating beef heifers. [Masters Thesis]. Texas A&M University; 2005. Available from: http://hdl.handle.net/1969.1/2796
Texas A&M University
16.
Volker, Ashley.
Combination Anthelmintics to Control Gastrointestinal Nematodes in Foals.
Degree: MS, Animal Science, 2010, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-34
► Two common nematodes that affect young horses are cyathostomes (small strongyles) and Parascaris equorum (ascarids). It has been recently found that populations of these nematodes…
(more)
▼ Two common nematodes that affect young horses are cyathostomes (small
strongyles) and Parascaris equorum (ascarids). It has been recently found that
populations of these nematodes are resistant to common anthelmintics used to control
them. Small strongyles have been found to be resistant to pyrantel and fenbendazole,
while ascarids have been found to be resistant to ivermectin. This represents a unique
dilemma in controlling the gastrointestinal nematode population in the foal. It has been
shown in other host species that combination anthelmintics can be used to successfully
treat resistant nematodes. The current study utilized 28 foals and was conducted from
April to November 2007. The foals were allocated into age cohorts and randomly
assigned a treatment regimen. Group I was administered ivermectin at 0.2 mg/kg BW.
Group II was administered ivermectin at 0.2-mg/kg BW and pyrantel pamoate at 6.6
mg/kg BW. Group III was administered ivermectin at 0.2-mg/kg BW and fenbendazole
at 10 mg/kg BW. Group IV was administered pyrantel pamoate at 6.6 mg/kg BW and
fenbendazole at 10 mg/kg BW. Fecal samples were collected at time of treatment and
two wk post treatment to determine effectiveness in removing egg producing adult nematodes. Each age cohort was then treated 30 d later with a different anthelmintic or
combination. That is, foals in group I were treated as those in group II, group II to
treatment III, group III to treatment IV, and group IV to treatment I. Over a period of 4
mo, each foal received at least one treatment in consecutive order.
The difference of egg counts (pre-treatment minus post-treatment) for small
strongyles treated with ivermectin (IVM) was 29.39 eggs per g (EPG), 5.44 EPG for
ivermectin with pyrantel (PRT), 3.85 EPG for ivermectin with fenbendazole (FBZ), and
-8.32 EPG for pyrantel with fenbendazole. There was a significant difference when
comparing IVM to IVM PRT (P = 0.0018), IVM vs. IVM FBZ (P = 0.0010), and IVM
vs. PRT FBZ (P < 0.0001). IVM was more effective than each of the other treatments.
There was no influence of treatment on ascarid EPG (P > 0.1184).
Advisors/Committee Members: Vogelsang, Martha M. (advisor), Coverdale, Josie A. (committee member), Craig, Thomas M. (committee member).
Subjects/Keywords: foals; anthelmintics; small strongyles; ascarids
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APA (6th Edition):
Volker, A. (2010). Combination Anthelmintics to Control Gastrointestinal Nematodes in Foals. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-34
Chicago Manual of Style (16th Edition):
Volker, Ashley. “Combination Anthelmintics to Control Gastrointestinal Nematodes in Foals.” 2010. Masters Thesis, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-34.
MLA Handbook (7th Edition):
Volker, Ashley. “Combination Anthelmintics to Control Gastrointestinal Nematodes in Foals.” 2010. Web. 18 Jan 2021.
Vancouver:
Volker A. Combination Anthelmintics to Control Gastrointestinal Nematodes in Foals. [Internet] [Masters thesis]. Texas A&M University; 2010. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-34.
Council of Science Editors:
Volker A. Combination Anthelmintics to Control Gastrointestinal Nematodes in Foals. [Masters Thesis]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-34
Texas A&M University
17.
Raymond, Russell Wayne.
Exploration of Potential Reservoir Hosts and Vectors of Leishmania in Nicaragua.
Degree: PhD, Wildlife and Fisheries Sciences, 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-3089
► Leishmaniasis is caused by infection with protozoan parasites within the genus Leishmania and, in the New World, is transmitted by the bites of female sand…
(more)
▼ Leishmaniasis is caused by infection with protozoan parasites within the genus
Leishmania and, in the New World, is transmitted by the bites of female sand flies
within the genus Lutzomyia. The occurrence of leishmaniasis in rodent species, the
geographic distribution of sand fly species in Nicaragua, and environmental factors
associated with the distribution of human cases of typical cutaneous leishmaniasis were
investigated. Three hundred ninety five rodents representing 17 species were collected
from 13 localities from August 2001–March 2006 and screened for Leishmania
infections. One Heteromys desmarestianus and one Peromyscus mexicanus were found
to be positive for leishmanial infections by PCR. This is the first report of Leishmania
infections in rodents in Nicaragua. Five hundred fifty six sand flies representing 12
species were collected from 8 localities, including Lutzomyia hartmanni, a new record
for this species in Nicaragua. The predominant sand fly species captured in western
Nicaragua were Lutzomyia longipalpis and Lutzomyia evansi. The predominant species
captured in central and eastern Nicaragua was Lutzomyia cruciata. The geographic distribution of sand flies in this study provides additional support to previouslypublished
reports of suspected vectors of Leishmania species that cause typical and
atypical forms of cutaneous leishmaniasis in Nicaragua.
Distribution data of human cases of typical cutaneous leishmaniasis obtained
from the Nicaraguan Ministry of Health, along with GIS and remotely sensed data of
elevation, precipitation, temperature, soil types and land use/cover classes, were used to
develop predictive logistic regression models for the presence or absence of human cases
within 151 municipalities. Mean annual precipitation and land use/cover were
determined to be the best environmental variable predictors for the occurrence of typical
cutaneous leishmaniasis.
Advisors/Committee Members: Dronen, Norman O. Jr. (advisor), Craig, Thomas M. (committee member), Olson, Jimmy K. (committee member), Peterson, Markus J. (committee member).
Subjects/Keywords: Leishmania; Leishmaniasis; Reservoirs; Vectors; Lutzomyia; sand flies; rodents; Nicaragua
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APA (6th Edition):
Raymond, R. W. (2009). Exploration of Potential Reservoir Hosts and Vectors of Leishmania in Nicaragua. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-3089
Chicago Manual of Style (16th Edition):
Raymond, Russell Wayne. “Exploration of Potential Reservoir Hosts and Vectors of Leishmania in Nicaragua.” 2009. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-3089.
MLA Handbook (7th Edition):
Raymond, Russell Wayne. “Exploration of Potential Reservoir Hosts and Vectors of Leishmania in Nicaragua.” 2009. Web. 18 Jan 2021.
Vancouver:
Raymond RW. Exploration of Potential Reservoir Hosts and Vectors of Leishmania in Nicaragua. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-3089.
Council of Science Editors:
Raymond RW. Exploration of Potential Reservoir Hosts and Vectors of Leishmania in Nicaragua. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-3089
Texas A&M University
18.
Johnsen, Mark Miller.
The status of resistance in Culex quinquefasciatus say (Diptera: culicidae) populations in Brazos and Harris Counties, Texas.
Degree: PhD, Entomology, 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-1298
► In 2002, West Nile virus was isolated for the first time in Harris County, Texas. The subsequent epidemic led the Harris County Mosquito Control Division…
(more)
▼ In 2002, West Nile virus was isolated for the first time in Harris County,
Texas.
The subsequent epidemic led the Harris County Mosquito Control Division to initiate an
extensive spraying operation to suppress infected adult mosquitoes. The control
program was aimed at the predominate disease-carrying mosquito for the southern
United States, Culex quinquefasciatus Say. With the increase of insecticide pressure on
the mosquito populations, the possibility of resistance was brought into question. A
three year study using a vial bioassay test was conducted in Harris (2004-2005) and
Brazos (2005-2006) counties to determine the resistance status of Cx. quinquefasciatus
to the six chemicals (malathion, naled, resmethrin, permethrin, sumithrin, and
pyrethrum) used most frequently in adult mosquito control programs.
The resistance ratios acquired from the vial bioassay tests were mapped onto
shapefiles for Harris and Brazos counties, which revealed clustering of areas with
pyrethroid resistance mosquito populations in the northeastern, southeastern, and
southwestern corners of Loop 610 in Harris County. An additional six-month
preliminary study, involving six operational areas in Harris County and three in Brazos County, was conducted, demonstrating only minor fluctuations in the monthly resistance
ratios occurring in both counties in 2005.
A significant correlation was documented between the two years of resistance
ratios for mosquitoes to the three pyrethroids in Harris County and all the insecticides
except pyrethrum in Brazos County. A significant relationship was also found between
the resmethrin resistance ratios and the number of spray events performed during the
previous year and the malathion resistance ratios with the insecticide treatments
conducted in the same year. The correlation analyses provide data used to predict areas
where resistance can develop in the mosquito population, thus providing the control
agency more data to plan future control tactics.
The overall analysis indicated that Harris County has localized pockets of
resistant mosquitoes; but, on a whole, it does not seem to have widespread resistance in
its mosquito populations. The only resistance that was detected was in the mosquitoes
tested against the three pyrethroids. Mosquitoes in Brazos County, which has no
organized mosquito control, demonstrated county-wide susceptibility to all six
insecticides tested.
Advisors/Committee Members: Olson, Jimmy K. (advisor), Craig, Thomas M. (committee member), Gorena, Roberto L. (committee member), Harris, Marvin K. (committee member), Russell, Leon H. (committee member).
Subjects/Keywords: Resistance; Mosquitoes
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APA (6th Edition):
Johnsen, M. M. (2009). The status of resistance in Culex quinquefasciatus say (Diptera: culicidae) populations in Brazos and Harris Counties, Texas. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1298
Chicago Manual of Style (16th Edition):
Johnsen, Mark Miller. “The status of resistance in Culex quinquefasciatus say (Diptera: culicidae) populations in Brazos and Harris Counties, Texas.” 2009. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-1298.
MLA Handbook (7th Edition):
Johnsen, Mark Miller. “The status of resistance in Culex quinquefasciatus say (Diptera: culicidae) populations in Brazos and Harris Counties, Texas.” 2009. Web. 18 Jan 2021.
Vancouver:
Johnsen MM. The status of resistance in Culex quinquefasciatus say (Diptera: culicidae) populations in Brazos and Harris Counties, Texas. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1298.
Council of Science Editors:
Johnsen MM. The status of resistance in Culex quinquefasciatus say (Diptera: culicidae) populations in Brazos and Harris Counties, Texas. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1298
Texas A&M University
19.
Kjos, Sonia Alane.
Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas.
Degree: PhD, Entomology, 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-1299
► Trypanosoma cruzi is endemic in the U.S., infecting humans, dogs, and wildlife. This study identified a new geographic distribution for triatomine species within Texas based…
(more)
▼ Trypanosoma cruzi is endemic in the U.S., infecting humans, dogs, and wildlife.
This study identified a new geographic distribution for triatomine species within
Texas
based on 2,449 records obtained from published data and new field studies. Triatomine
vectors of T. cruzi were reported from 97 counties covering all ecological zones.
Triatoma gerstaeckeri was the most commonly collected species followed by T.
sanguisuga. New field collections resulted in 233 specimens from 37 counties and a
52% T. cruzi infection rate. A second trypanosome, Blastocrithida triatomae was found
in two specimens from different locations. A habitat suitability model for T.
gerstaeckeri was developed using GIS and remote sensing applications. Forest and
rangeland were the predominant land cover classes found within T. gerstaeckeri habitat,
where as water and agriculture proved to have little influence on habitat suitability.
Genetic variation of seven triatomine species from
Texas was evaluated using
cytochrome b DNA sequences from 61 new specimens. This is the first study of the
taxonomic status of T. gerstaeckeri, T. indictiva, and T. neotomae using molecular markers. Intraspecific variation for T. sanguisuga and T. gerstaeckeri suggests
significant gene flow across their ranges within
Texas.
Genetic variation of T. cruzi isolates from
Texas was evaluated using SSU rRNA
gene sequences. Included were 63 new sequences from five triatomine species, canine,
baboon, and human isolates. Sequences partitioned into two groups in agreement with
previous studies on U.S. isolates. Genetic variation of T. cruzi did not occur according
to host, geographic location, or collection site.
The extent of Chagas disease in domestic canines of
Texas is described by
geographic distribution, signalment, and clinical presentation and histopathology.
Based on data from 553 cases, the geographic distribution in
Texas is widespread (46
counties) and closely matches the distribution of the Triatomine vectors. Chagas disease
was diagnosed in 33 breeds, primarily sporting/working dogs.
This study represents the most comprehensive characterization of components of
the Chagas disease transmission cycle in the U.S. to date. These findings should raise
awareness among physicians, veterinarians, and public health practitioners regarding T.
cruzi, its vectors, canine infection, and human risk for Chagas disease in
Texas.
Advisors/Committee Members: Coates, Craig J. (advisor), Olson, Jimmy K. (advisor), Craig, Thomas M. (committee member), Snowden, Karen F. (committee member), Teel, Pete D. (committee member).
Subjects/Keywords: Chagas disease; Trypanosoma cruzi; Triatoma; Triatominae
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APA ·
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APA (6th Edition):
Kjos, S. A. (2009). Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1299
Chicago Manual of Style (16th Edition):
Kjos, Sonia Alane. “Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas.” 2009. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-1299.
MLA Handbook (7th Edition):
Kjos, Sonia Alane. “Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas.” 2009. Web. 18 Jan 2021.
Vancouver:
Kjos SA. Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1299.
Council of Science Editors:
Kjos SA. Biogeography and genetic variation of triatomine chagas disease vectors and trypanosoma cruzi isolates from texas. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1299
. 
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