+publisher:"Texas A&M University" +contributor:("Adams, L. Garry")

Texas A&M University

1. Schutta, Christopher John. Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis.

Degree: PhD, Veterinary Microbiology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1874

► Previous analysis of the bovine SLC11A1 complementary DNA (cDNA) failed to identify any nucleotide variations other than a microsatellite length variation within the 3' untranslated… (more)

▼ Previous analysis of the bovine SLC11A1 complementary DNA (cDNA) failed to identify any nucleotide variations other than a microsatellite length variation within the 3' untranslated region functionally associated with bovine brucellosis. In this study I set out to identify mutations in the genomic complement of the gene that may be associated with resistance or susceptibility to bovine brucellosis, and to determine if the microsatellite length polymorphism in the 3'UTR of bovine SLC11A1 modulates gene expression and subsequent disease resistance in a phase dependent manner. The results of this study demonstrate that there are seventy-five total single nucleotide polymorphic (SNP) sites (excluding indels) located within the bovine genomic SLC11A1 sequence of a Brucella abortus resistant bull and a susceptible cow. Twenty of these SNPs segregated between resistant and susceptible populations, with 3 non-synonymous SNPs significantly associating with resistance or susceptibility to B. abortus infection. An A695G within exon 2 resulted in a histidine (resistant allele) to arginine (susceptible allele) amino acid substitution and was in significant linkage disequilibrium with the previously described 3' untranslated region (UTR) microsatellite length variation associated with brucellosis resistance. A transcriptional element search in the 3' UTR revealed a ETS-domain PU.1 site, an IFN-γ activation site (GAS), an Interferon Consensus Sequence Binding Protein site (ICSBP) and several Initiation Response sites (Inr), suggesting a possible function for this region in regulation of the expression of SLC11A1. A mobility shift assay confirmed sequence-specific DNA-protein interaction within this region. A luciferase reporter assay indicated that the 3'UTR of SLC11A1 could act as a downstream enhancer for expression. Macrophage killing assays with RAW264.7 cells expressing bovine SLC11A1 demonstrated that the microsatellite repeat is functionally associated with the macrophage killing efficiency, but not in a phase-dependent manner, suggesting that these length polymorphisms do not affect the angular orientation between cooperatively binding transcription factors, and leaves the possibility that the 3'UTR microsatellites regulate SLC11A1 transcription through some alternate mechanism, possibly mRNA stability. Advisors/Committee Members: Templeton, Joe W. (advisor), Adams, L. Garry (committee member), Davis, Donald S. (committee member), Derr, James N. (committee member).

Subjects/Keywords: SLC11A1; brucellosis; natural disease resistance

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APA (6^th Edition):

Schutta, C. J. (2009). Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1874

Chicago Manual of Style (16^th Edition):

Schutta, Christopher John. “Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis.” 2009. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1874.

MLA Handbook (7^th Edition):

Schutta, Christopher John. “Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis.” 2009. Web. 28 Feb 2021.

Vancouver:

Schutta CJ. Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1874.

Council of Science Editors:

Schutta CJ. Bovine SLC11A1: genomic sequence variation and functional analysis in cattle naturally resistant and susceptible to bovine brucellosis. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1874

Texas A&M University

2. Bissett, Wesley Thurlow, Jr. Ecosystem health at the texas coastal bend: a spatial analysis of exposure and response.

Degree: PhD, Veterinary Microbiology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2126

► This dissertation investigated locational risks to ecosystem health associated with proximity to industrial complexes. The study was performed at the behest of ranchers and citizens… (more)

▼ This dissertation investigated locational risks to ecosystem health associated with proximity to industrial complexes. The study was performed at the behest of ranchers and citizens living and working down-prevailing wind from the Formosa Plastics, Inc. and ALCOA facilities located in Calhoun County, Texas. Concerns expressed were for potential genotoxicity resulting from exposure to complex chemical mixtures released by the facilities. Exposure assessment of the marine environment was performed with sediments and oysters from Lavaca Bay being analyzed. Numerous chemicals were found to be present at concentrations considered likely to result in adverse responses in exposed populations. Bayesian geostatistical analysis was performed to determine if the concentrations were affected by a spatial process. Mercury and polycyclic aromatic hydrocarbons were the most notable of the chemicals found to be present at elevated concentrations and affected by a spatial process. Evaluation of maps generated from spatial modeling revealed that proximity to ALCOA resulted in elevated risks for exposure to harmful concentrations of pollutants. Genotoxicity was measured in two sentinel species. Oysters (Crassostrea virginica) were utilized for evaluation of the marine environment and cattle (Bos taurus and Bos taurus crossbred cattle) were chosen for evaluation of the terrestrial environment. Chromosomal aberration analysis was performed on oyster hematocytes. Analysis of the results failed to demonstrate the presence of an important generalized spatial process but some specific locations close to the ALCOA plant had elevations in this measure of genotoxicity. Stress as measured by the lysosomal destabilization assay was also performed on oyster hematocytes. These results were found to be affected by a significant spatial process with the highest degree of destabilization occurring in close proximity to ALCOA. Genotoxicity in cattle was evaluated with the single cell gel electrophoresis assay and chromosomal aberration analysis. Bayesian geostatistical analyis revealed the presence of important spatial processes. DNA-protein cross-linkage was the most notable with a strong indication of increased damage down-prevailing wind from the industrial complexes. Results indicated that proximity to industrial facilities increased the risk for harmful exposures, genotoxicity, and lysosomal destabilization. Advisors/Committee Members: Adams, L. Garry (advisor), Thompson, James A. (advisor), Field, Robert (committee member), Moyer, William (committee member), Phillips, Timothy (committee member), Scott, H. Morgan (committee member).

Subjects/Keywords: genotoxicity; sentinnel species; biomarkers; spatial analysis; Bayesian analysis

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APA (6^th Edition):

Bissett, Wesley Thurlow, J. (2009). Ecosystem health at the texas coastal bend: a spatial analysis of exposure and response. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2126

Chicago Manual of Style (16^th Edition):

Bissett, Wesley Thurlow, Jr. “Ecosystem health at the texas coastal bend: a spatial analysis of exposure and response.” 2009. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2126.

MLA Handbook (7^th Edition):

Bissett, Wesley Thurlow, Jr. “Ecosystem health at the texas coastal bend: a spatial analysis of exposure and response.” 2009. Web. 28 Feb 2021.

Vancouver:

Bissett, Wesley Thurlow J. Ecosystem health at the texas coastal bend: a spatial analysis of exposure and response. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2126.

Council of Science Editors:

Bissett, Wesley Thurlow J. Ecosystem health at the texas coastal bend: a spatial analysis of exposure and response. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2126

Texas A&M University

3. Gull, Tamara Brownsey. In vivo infection biology of contagious bovine pleuropneumonia.

Degree: PhD, Veterinary Microbiology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2611

► Contagious Bovine Pleuropneumonia (CBPP), caused by Mycoplasma mycoides mycoides small colony (MmmSC), is a devastating respiratory disease of cattle in Africa, Asia and the Middle… (more)

Subjects/Keywords: Contagious Bovine Pleuropneumonia; Mycoplasma mycoides mycoides small colony; cattle; infectious disease; foreign animal disease

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APA (6^th Edition):

Gull, T. B. (2009). In vivo infection biology of contagious bovine pleuropneumonia. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2611

Chicago Manual of Style (16^th Edition):

Gull, Tamara Brownsey. “In vivo infection biology of contagious bovine pleuropneumonia.” 2009. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2611.

MLA Handbook (7^th Edition):

Gull, Tamara Brownsey. “In vivo infection biology of contagious bovine pleuropneumonia.” 2009. Web. 28 Feb 2021.

Vancouver:

Gull TB. In vivo infection biology of contagious bovine pleuropneumonia. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2611.

Council of Science Editors:

Gull TB. In vivo infection biology of contagious bovine pleuropneumonia. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2611

Texas A&M University

4. Sivula, Christine Patricia. Comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice.

Degree: MS, Laboratory Animal Medicine, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2989

► Salmonellosis is one of the most important bacterial food borne illnesses worldwide. Among the many Salmonella serotypes, Typhimurium is the most commonly implicated serotype in… (more)

▼ Salmonellosis is one of the most important bacterial food borne illnesses worldwide. Among the many Salmonella serotypes, Typhimurium is the most commonly implicated serotype in human disease in the United States. A major source of infection for humans is consumption of chicken or egg products that have been contaminated with S. Typhimurium. The breadth of knowledge regarding colonization and persistence factors in the chicken is small when compared to our knowledge of factors that are important for these processes in other species used in Salmonella research, such as cattle and mice. Defining the factors important for these processes in the chick is the first step in decreasing the transmission of Salmonella between animal and human hosts. In this work, we developed a chicken model to identify and study intestinal colonization and persistence factors of Salmonella enterica serovar Typhimurium. We studied the degree of enteric and systemic colonization of wild type S. Typhimurium ATCC14028, one of the most widely studied Typhimurium isolates, in White Leghorn chicks and in Salmonella-resistant CBA/J mice during infection. Furthermore, we determined the distribution of wild type S. Typhimurium and a SPI-1 mutant (invA) during competitive infection in the cecum of 1-week-old chicks and 8-week-old mice. Cell associated, intracellular and luminal distributions of these strains in the cecum were analyzed as total counts in each compartment and also as a competitive index. Localization of S. Typhimurium ATCC14028 and the role of SPI-1 in colonization are well studied in murine models of infection, but comparative infection in chicks with the same strain has not been undertaken previously. We show that the cecal contents are the major site for recovery of S. Typhimurium in the cecum of 1-week-old chicks and Salmonella-resistant mice. We also show that while SPI-1 is important for successful infection in the murine model, it is important only for cell association in the cecum of 1-week-old chicks. Finally, we found that in chicks infected at 1 week of age, bacterial counts in the feces do not reflect those seen in the cecum as they do in mice. Advisors/Committee Members: Adams, L. Garry (advisor), Andrews-Polymenis, Helene L. (advisor), Ihrig, Melanie (committee member).

Subjects/Keywords: Salmonella; cecum; SPI-1; chicks; mice

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APA (6^th Edition):

Sivula, C. P. (2009). Comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2989

Chicago Manual of Style (16^th Edition):

Sivula, Christine Patricia. “Comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice.” 2009. Masters Thesis, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2989.

MLA Handbook (7^th Edition):

Sivula, Christine Patricia. “Comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice.” 2009. Web. 28 Feb 2021.

Vancouver:

Sivula CP. Comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice. [Internet] [Masters thesis]. Texas A&M University; 2009. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2989.

Council of Science Editors:

Sivula CP. Comparison of cecal colonization of Salmonella enterica serotype Typhimurium in white leghorn chicks and Salmonella-resistant mice. [Masters Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2989

Texas A&M University

5. Rossetti, Carlos Alberto. Host and pathogen transcriptional profiles of acute Brucella melitensis infection.

Degree: PhD, Veterinary Microbiology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1636

► The parallel gene expression profiles of Brucella melitensis and the host have not been elaborated. In this study, I analyze and discuss the transcriptional profiles… (more)

▼ The parallel gene expression profiles of Brucella melitensis and the host have not been elaborated. In this study, I analyze and discuss the transcriptional profiles of B. melitensis invasive-associated genes, the expression profile of intracellular B. melitensis and B. melitensis-infected non-phagocytic cells in the first 12 h post-infection (PI), and the in vivo temporal global transcriptome of both B. melitensis and the infected bovine host in the first 4 h PI. The initial study found that B. melitensis at late-log phase of growth were more invasive in non-phagocytic cells than at early-log or stationary growth phase. Microarray-based studies identified 454 Brucella genes differentially expressed between the most and the least invasive growth phases. Additionally, B. melitensis strains with transposon interrupted in loci BMEII0380 (acrA) and BMEI1538 (hypothetical protein) were found to be deficient in internalization compare with the wild-type strain. A second experiment was designed with the goal of characterizing host and pathogen transcriptome in parallel. For detecting intracellular Brucella gene expression, a combined protocol consisting of a linear amplification of sense-stranded RNA biased to pathogen transcripts to the previously enriched host:pathogen RNA mixed sample, was developed. RNA samples were hybridized on human and Brucella cDNA microarrays, which analysis revealed a common down-regulation transcriptional profile at 4 h PI that was reverse at 12 h PI. The integrity of B. melitensis virB operon and the expression of host MAPK1 were confirmed as critical for early B. melitensis intracellular survival and replication in non-phagocytic cells. Finally, a temporal morphological and molecular characterization of the initial B. melitensis:bovine host interaction using a calf ileal loop model was performed. B. melitensis was isolated from intestinal Peyer’s patches as soon as 15 min and from systemic blood after 30 min postintra luminal inoculation. Microarray results revealed a common transcriptional profile in Brucella, but two different transcriptional profiles were identified in the host in the first 4 h PI. The importance of differentially expressed biological processes, pathways and individual genes in the initial Brucella pathogenesis is discussed. Advisors/Committee Members: Adams, L. Garry (advisor), Thomas, Terry L. (committee member), Tsolis, Renee M. (committee member), Womack, James E. (committee member).

Subjects/Keywords: Brucella; Bovine; Gene expression; Microarrays

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APA (6^th Edition):

Rossetti, C. A. (2009). Host and pathogen transcriptional profiles of acute Brucella melitensis infection. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1636

Chicago Manual of Style (16^th Edition):

Rossetti, Carlos Alberto. “Host and pathogen transcriptional profiles of acute Brucella melitensis infection.” 2009. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1636.

MLA Handbook (7^th Edition):

Rossetti, Carlos Alberto. “Host and pathogen transcriptional profiles of acute Brucella melitensis infection.” 2009. Web. 28 Feb 2021.

Vancouver:

Rossetti CA. Host and pathogen transcriptional profiles of acute Brucella melitensis infection. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1636.

Council of Science Editors:

Rossetti CA. Host and pathogen transcriptional profiles of acute Brucella melitensis infection. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1636

6. Figueiredo, Josely Ferreira. Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses.

Degree: PhD, Veterinary Microbiology, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1385

► We demonstrated that infection of HeLa cells, which are non-responsive to flagellin, with wild type Salmonella enterica serotype Typhimurium (S. typhimurium) activated chemokine expression at… (more)

▼ We demonstrated that infection of HeLa cells, which are non-responsive to flagellin, with wild type Salmonella enterica serotype Typhimurium (S. typhimurium) activated chemokine expression at higher level than S. typhimurium lacking sipAsopABDE2, indicating that the corresponding effector proteins (SipA, SopA, SopB, SopD and SopE2) are required to induce chemokines independent of flagellin. The S. typhimurium sipAsopABDE2 mutant complemented with sipA activated IL-8 expression at significantly higher level than a S. typhimurium sipAsopABDE2 mutant. However, extracellular addition of recombinant SipA failed to induce IL-8. Phosphorylation analyses demonstrated that S. typhimurium carrying a chromosomal copy of sipA (sopABDE2 mutant) induced phosphorylation of CREB1, JUN and p38MAPK, which are proteins involved in IL-8 expression. The contribution of effector proteins to S. typhimurium-induced intracellular Ca2+ mobilization and its role in IL-8 expression and bacterial internalization were also investigated. Our results demonstrated that wild type S. typhimurium significantly increased the amplitude of intracellular Ca2+ beginning 30 sec after infection. However, further analyses of intracellular Ca2+ changes in HeLa cells infected with S. typhimurium mutants indicated no correlation between increased intracellular Ca2+ and IL-8 expression or bacterial internalization. To analyze specific cell populations targeted by wild type S. typhimurium or S. typhimurium carrying a chromosomal copy of sipA (sopABDE2 mutant), laser capture microdissection was performed. Our data indicated that in wild type S. typhimurium-infected bovine Peyer’s patches, high levels of IL-8 were expressed in enterocytes of crypts, whereas Gro-α was expressed in enterocytes of both crypts and absorptive villi. A strain carrying a chromosomal copy of sipA colonized the same cell population as wild type, but induced IL8 and Gro-α in enterocytes of both crypts and absorptive villi. In conclusion, we demonstrated that in vitro S. typhimurium effector proteins induce chemokine expression independent of Ca2+ changes through phosphorylation of proteins related to IL-8 pathway. In vivo, we found higher levels of IL-8 expression in enterocytes of crypts than enterocytes of absorptive villi, although both cell populations contributed to Gro-α expression. These data extend the knowledge of the molecular mechanism by which S. typhimurium induces inflammatory genes by identifying pathogen and host molecules involved in inflammation. Advisors/Committee Members: Adams, L. Garry (advisor), Baeumler, Andreas (committee member), Burghardt, Robert (committee member), Tsolis, Renee (committee member).

Subjects/Keywords: Salmonella; inflammation

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APA (6^th Edition):

Figueiredo, J. F. (2009). Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1385

Chicago Manual of Style (16^th Edition):

Figueiredo, Josely Ferreira. “Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses.” 2009. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1385.

MLA Handbook (7^th Edition):

Figueiredo, Josely Ferreira. “Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses.” 2009. Web. 28 Feb 2021.

Vancouver:

Figueiredo JF. Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses. [Internet] [Doctoral dissertation]. Texas A&M University; 2009. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1385.

Council of Science Editors:

Figueiredo JF. Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses. [Doctoral Dissertation]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1385

Texas A&M University

7. Kahl, Melissa Marie. Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models.

Degree: PhD, Veterinary Microbiology, 2006, Texas A&M University

URL: http://hdl.handle.net/1969.1/4197

► Historical data suggests that prolonged survival of Brucella vaccine organisms in the target host enhances immune protection. Recent research has focused upon the development of… (more)

Subjects/Keywords: Brucella; vaccine

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APA (6^th Edition):

Kahl, M. M. (2006). Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4197

Chicago Manual of Style (16^th Edition):

Kahl, Melissa Marie. “Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models.” 2006. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/4197.

MLA Handbook (7^th Edition):

Kahl, Melissa Marie. “Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models.” 2006. Web. 28 Feb 2021.

Vancouver:

Kahl MM. Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models. [Internet] [Doctoral dissertation]. Texas A&M University; 2006. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/4197.

Council of Science Editors:

Kahl MM. Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models. [Doctoral Dissertation]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/4197

Texas A&M University

8. Taylor, Kristen Hawkins. Genetic analyses of bovine CARD15, a putative disease resistance gene.

Degree: PhD, Genetics, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/219

► Through a binding partner the CARD15 gene activates NF-kB, a molecule with a role in the initiation of the inflammatory immune response. The gene is… (more)

▼ Through a binding partner the CARD15 gene activates NF-kB, a molecule with a role in the initiation of the inflammatory immune response. The gene is highly conserved in both structure and function in human and mouse and has recently been implicated as a disease resistance gene in Crohn's disease and Blau Syndrome in human. The gene's relationship to disease and its conservation between species suggests that it may also have a conserved role in bovine disease resistance. To elucidate the potential role of bovine CARD15 in disease resistance, the gene was characterized in cattle. Bovine CARD15 is located 4.2 cR5000 telomeric to ADCY7 on chromosome 18. It spans ~30 kb and is comprised of 12 exons, 11 of which are coding. Bovine CARD15 is expressed in many tissues, but is most abundant in peripheral blood leukocytes. An extensive comparative analysis between the bovine, mouse and human CARD15 genes revealed high levels of inter-species conservation in sequence, genomic structure and protein domains. Conserved putative regulatory motifs were identified in the three species comparison of the 5'UTR, 3'UTR and the intronic sequences flanking exons. Additionally, diverse regulatory motifs were identified in each of the species indicating an evolutionary divergence in the mechanisms of regulation of gene expression. To assess the extent of genetic diversity within bovine CARD15, 41 individuals from nine breeds representing two subspecies were sequenced and screened for polymorphisms. Thirty-six single nucleotide polymorphisms (SNPs) were identified including 26 within the gene transcript. Haplotypes were estimated for each individual and parsimonious SNP sets were identified with which the multi-locus Bos taurus and Bos indicus haplotypes may be reconstructed. There was a significantly higher rate of substitutions within Bos indicus than in Bos taurus. A significantly higher rate of nonsynonymous to synonymous substitutions was found in Bos taurus indicating that positive Darwinian selection is acting on the gene within this subspecies. Association analyses were performed between these SNP loci and haplotypes with Johne's disease. No overwhelming evidence for a simple causal relationship was detected. Assays are provided to screen populations of cattle for variation in the CARD15 gene. Advisors/Committee Members: Womack, James E. (advisor), Derr, James N. (committee member), Adams, L. Garry (committee member), Roussel, Allen J. (committee member).

Subjects/Keywords: CARD15; NOD2; bovine; disease resistance; Crohn's; Johne's

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APA (6^th Edition):

Taylor, K. H. (2004). Genetic analyses of bovine CARD15, a putative disease resistance gene. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/219

Chicago Manual of Style (16^th Edition):

Taylor, Kristen Hawkins. “Genetic analyses of bovine CARD15, a putative disease resistance gene.” 2004. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/219.

MLA Handbook (7^th Edition):

Taylor, Kristen Hawkins. “Genetic analyses of bovine CARD15, a putative disease resistance gene.” 2004. Web. 28 Feb 2021.

Vancouver:

Taylor KH. Genetic analyses of bovine CARD15, a putative disease resistance gene. [Internet] [Doctoral dissertation]. Texas A&M University; 2004. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/219.

Council of Science Editors:

Taylor KH. Genetic analyses of bovine CARD15, a putative disease resistance gene. [Doctoral Dissertation]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/219

Texas A&M University

9. Endicott-Yazdani, Tiana. Identification of Novel Salmonella Typhimurium Genes Required For Survival During Intestinal Inflammation.

Degree: PhD, Microbial & Molecular Pathogenesis, Texas A&M University

URL: http://hdl.handle.net/1969.1/154260

► Bovine ligated ileal loops provide the best model to examine Salmonella Typhimurium genes required for survival during the early stages of infection, as humans and… (more)

▼ Bovine ligated ileal loops provide the best model to examine Salmonella Typhimurium genes required for survival during the early stages of infection, as humans and cattle develop very similar intestinal pathogenesis in response to the organism. Utilizing pools of mutants, both single gene and multi-gene deletion mutants, much of the S. Typhimurium genome can be screened simultaneously. A library consisting of multi-gene deletion mutants was screened in ligated ileal loops in calves. Regions of the genome required for survival in this model were identified in mucus and tissue samples using microarray analysis. STM1187-90 was one such region identified as under selection in both mucus and tissue. A ASTM1188 mutant was confirmed to poorly colonize the intestinal mucus and tissue in the presence of inflammation in competitive infections with the wild type. Complementation in trans reversed the phenotype of the ASTM1188 mutant. The phenotype of the ASTM1188 mutant was replicated and complemented in trans in the murine colitis model. STM1188 is a Salmonella specific gene whose protein product we show to be located in the inner membrane. This gene is absent in host-adapted serovars S. Typhi and S. Paratyphi A. Mutation of the putative lipobox cysteine to alanine resulted in mis- localization of STM1188C24A to the cytoplasm, and the inability to complement ASTM1188 in trans in mice. A second screen of a single gene deletion pool (SGD) identified many novel genes with potential roles during inflammation as well as many predicted genes with defined roles during the early stages of infection. Several novel gene phenotypes were confirmed and subsequently complemented in competitive infections with wild type. AhilE was identified during SGD screening and confirmed in bovine ligated ileal loops as being selected against during competitive infections with extensive inflammation, however, it was not selected against when the inflammatory immune response was limited. HilE has been previously shown to negatively regulate SPI-1 expression. Utilizing the murine colitis model, the AhilE phenotype was confirmed and complemented during competitive infection with wild type. By using (3-galactosidase assays, AhilE was confirmed to overexpress SPI-1, however, surprisingly AhilE also overexpressed SPI-2 during SPI-1 inducing conditions. In the current studies we performed Salmonella screens in bovine ligated ileal loops and confirmed novel virulence genes required for survival during inflammation. Advisors/Committee Members: Adams, L. Garry (committee member), Skare, Jonathon (committee member), Tesh, Vernon (committee member), Andrews-Polymenis, Helene (committeechair).

Subjects/Keywords: Biomedical Sciences

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APA (6^th Edition):

Endicott-Yazdani, T. (n.d.). Identification of Novel Salmonella Typhimurium Genes Required For Survival During Intestinal Inflammation. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/154260

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Chicago Manual of Style (16^th Edition):

Endicott-Yazdani, Tiana. “Identification of Novel Salmonella Typhimurium Genes Required For Survival During Intestinal Inflammation.” Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/154260.

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MLA Handbook (7^th Edition):

Endicott-Yazdani, Tiana. “Identification of Novel Salmonella Typhimurium Genes Required For Survival During Intestinal Inflammation.” Web. 28 Feb 2021.

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Vancouver:

Endicott-Yazdani T. Identification of Novel Salmonella Typhimurium Genes Required For Survival During Intestinal Inflammation. [Internet] [Doctoral dissertation]. Texas A&M University; [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/154260.

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Council of Science Editors:

Endicott-Yazdani T. Identification of Novel Salmonella Typhimurium Genes Required For Survival During Intestinal Inflammation. [Doctoral Dissertation]. Texas A&M University; Available from: http://hdl.handle.net/1969.1/154260

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