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You searched for +publisher:"Technical University of Lisbon" +contributor:("Silva, Manuela"). Showing records 1 – 3 of 3 total matches.

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Technical University of Lisbon

1. Tomás, Diana Raquel dos Santos. Efeitos do stress térmico na transcrição e topologia nuclear de sequências repetitivas.

Degree: 2010, Technical University of Lisbon

Mestrado em Biologia Funcional - Instituto Superior de Agronomia

The aim of this work was to evaluate the influence of high temperature stress (HTS) in the chromatin organization of two repetitive sequences – 45S ribossomal DNA and subtelomeric pSc200 – and of nucleoli in interphase root tips and coleoptiles nuclei from Secale cereale seedlings using fluorescent in situ hybridization and imunocitochemestry, respectively. Furthermore we analyzed the influence of high temperature stress in the transcription patterns of the same sequences through quantitative real-time PCR. Nuclei from both organs show different conformation patters. Root tips nuclei have bigger dimensions, more disperse subtelomeric FISH signals and more compact ribossomal probe. We observe that high temperature stress causes chromatin decondensation in both sequences in root tips nuclei, while in coleoptile nuclei, beside the rDNA decondensation, we observe an increase in the nuclear area suggesting a global nuclear decompactation. The nucleolar area is also higher in both types of nuclei of stressed seedlings. This increase could be correlated with the increase in the ribossomal genes expression level induced by temperature stress, demonstrated through real-time PCR. The present work describes for the first time that the subtelomeric sequence pSc200 is transcribed, although its levels are not significantly altered with high temperature stress.

Advisors/Committee Members: Silva, Manuela, Viegas, Wanda.

Subjects/Keywords: secale cereale; high temperature stress; ribossomal DNA; subtelomeric pSc200; nucleolus; stress de alta temperatura; DNA ribossomal; subtelomerica pSc200

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Tomás, D. R. d. S. (2010). Efeitos do stress térmico na transcrição e topologia nuclear de sequências repetitivas. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/2974

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Tomás, Diana Raquel dos Santos. “Efeitos do stress térmico na transcrição e topologia nuclear de sequências repetitivas.” 2010. Thesis, Technical University of Lisbon. Accessed April 19, 2019. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/2974.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Tomás, Diana Raquel dos Santos. “Efeitos do stress térmico na transcrição e topologia nuclear de sequências repetitivas.” 2010. Web. 19 Apr 2019.

Vancouver:

Tomás DRdS. Efeitos do stress térmico na transcrição e topologia nuclear de sequências repetitivas. [Internet] [Thesis]. Technical University of Lisbon; 2010. [cited 2019 Apr 19]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/2974.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomás DRdS. Efeitos do stress térmico na transcrição e topologia nuclear de sequências repetitivas. [Thesis]. Technical University of Lisbon; 2010. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/2974

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Technical University of Lisbon

2. Bento, Miguel Ângelo Martins Oliveira. Characterization of genomic polyploids plasticity in the wheat-rye system.

Degree: 2011, Technical University of Lisbon

Doutoramento em Biologia - Instituto Superior de Agronomia

Polyploidization is a remarkable driving force in plant evolution, where hybridization and chromosome doubling result in huge genomic stress. Deeper knowledge about parental genomes behaviour in a hybrid nucleus and on processes underlying genetic and epigenetic modifications induced by polyploidization is essential to understand such evolutive process. Using triticale as model species we evaluated the impact of polyploidization through molecular and cytological approaches. Genomic rearrangements involving retrotransposons and microsatellites related sequences include both repetitive and coding sequences, and revealed a preferential loss of rye-origin bands. Chromosome distribution of such marker sequences demonstrated moreover enrichment in heterochromatic domains. Intensification of rye genome rearrangements was also disclosed in wheat lines with disomic additions of rye chromosomes, probably due to unbalanced genomic constitution. An integrative review of genomic modulation evaluated in Triticeae hybrid/polyploidy species unravelled furthermore higher restructuring of larger parental genomes, emphasizing the importance of genome size homogenization. Epigenetic analysis of nucleolar dominance in wheat addition line with rye nucleolar chromosomes revealed an unexpected up-regulation of ribosomal genes from wheat-origin, suggesting that mutual and opposite expression patterns modifications are induced by genome interactions. Altogether we demonstrate that heterochromatic domains are highly involved in parental genomes adjustments required to polyploids stabilization. –  –  –  –  –  –  –  –  –  –  –  –  –  –  – A poliploidização é uma das principais forças evolutivas em plantas, sendo essencial um conhecimento profundo dos processos subjacentes às alterações genómicas e epigenéticas associadas a este processo evolutivo. O triticale foi utilizado como espécie modelo para avaliar o impacto da poliploidização utilizando técnicas moleculares e citológicas. Identificaram-se rearranjos genómicos envolvendo sequências repetitivas e codificantes associadas a retrotransposões e microssatélites, afectando preferencialmente o genoma de centeio. A distribuição cromossómica dessas sequências demonstrou a sua predominância em domínios heterocromáticos. Paralelamente, em linhas de trigo com a adição dos cromossomas de centeio, observou-se uma intensificação de rearranjos nesse genoma, provavelmente resultante do desequilíbrio genómico parental. A integração dos estudos realizados em híbridos/poliplóides pertencentes à tribo Triticeae demonstrou a reestruturação preferencial do genoma parental maior, realçando a importância da homogeneização genómica. A análise epigenética dos genes ribossomais na linha de trigo com introgressão de cromossomas nucleolares de centeio, indica que o processo de dominância nucleolar induz modificações mútuas dos padrões de expressão genica dos progenitores. Este trabalho enfatiza assim a importância da modulação dos domínios heterocromáticos…

Advisors/Committee Members: Silva, Manuela, Viegas, Wanda.

Subjects/Keywords: triticeae; triticale; wheat; rye; polyploidization; retrotransposons; microsatellites; genome restructuring; rADN expression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bento, M. . M. O. (2011). Characterization of genomic polyploids plasticity in the wheat-rye system. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/3833

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bento, Miguel Ângelo Martins Oliveira. “Characterization of genomic polyploids plasticity in the wheat-rye system.” 2011. Thesis, Technical University of Lisbon. Accessed April 19, 2019. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/3833.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bento, Miguel Ângelo Martins Oliveira. “Characterization of genomic polyploids plasticity in the wheat-rye system.” 2011. Web. 19 Apr 2019.

Vancouver:

Bento MMO. Characterization of genomic polyploids plasticity in the wheat-rye system. [Internet] [Thesis]. Technical University of Lisbon; 2011. [cited 2019 Apr 19]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/3833.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bento MMO. Characterization of genomic polyploids plasticity in the wheat-rye system. [Thesis]. Technical University of Lisbon; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/3833

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Technical University of Lisbon

3. Rodrigues, Carlos Alberto Farinha. Comportamentos de despesa e poupança das famílias urbanas em Portugal: uma aplicação do sistema linear de despesa generalizado.

Degree: 1989, Technical University of Lisbon

Mestrado em Economia

N/A

Advisors/Committee Members: Silva, Manuela.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Rodrigues, C. A. F. (1989). Comportamentos de despesa e poupança das famílias urbanas em Portugal: uma aplicação do sistema linear de despesa generalizado. (Thesis). Technical University of Lisbon. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13250

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Rodrigues, Carlos Alberto Farinha. “Comportamentos de despesa e poupança das famílias urbanas em Portugal: uma aplicação do sistema linear de despesa generalizado.” 1989. Thesis, Technical University of Lisbon. Accessed April 19, 2019. https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13250.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Rodrigues, Carlos Alberto Farinha. “Comportamentos de despesa e poupança das famílias urbanas em Portugal: uma aplicação do sistema linear de despesa generalizado.” 1989. Web. 19 Apr 2019.

Vancouver:

Rodrigues CAF. Comportamentos de despesa e poupança das famílias urbanas em Portugal: uma aplicação do sistema linear de despesa generalizado. [Internet] [Thesis]. Technical University of Lisbon; 1989. [cited 2019 Apr 19]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13250.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodrigues CAF. Comportamentos de despesa e poupança das famílias urbanas em Portugal: uma aplicação do sistema linear de despesa generalizado. [Thesis]. Technical University of Lisbon; 1989. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13250

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.