Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for +publisher:"St. Cloud State University" +contributor:("Nathan Bruender"). Showing records 1 – 3 of 3 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters

1. Schorr, Anna L. Yeast Two Hybrid Screen of a Putative Toxoplasma gondii Cyclin, TGME49_266900.

Degree: Biological Sciences - Cell and Molecular: M.S., Biology, 2018, St. Cloud State University

In this current research, protein-protein interactions with a putative Toxoplasma gondii cyclin, TGME49_266900 or "Cyc6," were discovered via a yeast two hybrid screen. Several putative interacting protein partners were isolated and described from a cDNA library of an asynchronous tachyzoite transcriptome. After false positives were weaned from the study, two proteins were identified as Cyc6 interacting partners. These two proteins are described from the toxodb.org bioinformatic database as a DJ-1 family protein (TGME49_214290) and a ThiF protein (TGME49_314890). Interestingly, the interacting DJ-1 protein has been shown in previous research to play a role in T. gondii microneme secretion. Additionally, ThiF proteins share distinct traits with E1 enzymes at the start of the ubiquitin pathway in eukaryotes. After no evidence of an interacting CDK partner for Cyc6 was obtained, a direct experiment was conducted testing for an interaction between Cyc6 and a putative CDK with expression levels notably higher in bradyzoites instead of tachyzoites. The outcome of this experiment showed no interaction between Cyc6 and the putative CDK. Although no interacting CDK partner was evident from this yeast two hybrid screen, two proteins were found to display a strong and biologically relevant interaction with the putative cyclin of interest. Future studies regarding Cyc6 should explore potential noncanonical roles for this putative cyclin in not only tachyzoites, but also bradyzoites and the purpose of Cyc6's interaction with the two proteins discovered from this screen. Advisors/Committee Members: Christopher Kvaal, Timothy Schuh, Nathan Bruender.

Subjects/Keywords: Toxoplasma gondii; cyclin; yeast two hybrid screen; cyclin dependent kinase

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Schorr, A. L. (2018). Yeast Two Hybrid Screen of a Putative Toxoplasma gondii Cyclin, TGME49_266900. (Masters Thesis). St. Cloud State University. Retrieved from https://repository.stcloudstate.edu/biol_etds/31

Chicago Manual of Style (16th Edition):

Schorr, Anna L. “Yeast Two Hybrid Screen of a Putative Toxoplasma gondii Cyclin, TGME49_266900.” 2018. Masters Thesis, St. Cloud State University. Accessed January 19, 2021. https://repository.stcloudstate.edu/biol_etds/31.

MLA Handbook (7th Edition):

Schorr, Anna L. “Yeast Two Hybrid Screen of a Putative Toxoplasma gondii Cyclin, TGME49_266900.” 2018. Web. 19 Jan 2021.

Vancouver:

Schorr AL. Yeast Two Hybrid Screen of a Putative Toxoplasma gondii Cyclin, TGME49_266900. [Internet] [Masters thesis]. St. Cloud State University; 2018. [cited 2021 Jan 19]. Available from: https://repository.stcloudstate.edu/biol_etds/31.

Council of Science Editors:

Schorr AL. Yeast Two Hybrid Screen of a Putative Toxoplasma gondii Cyclin, TGME49_266900. [Masters Thesis]. St. Cloud State University; 2018. Available from: https://repository.stcloudstate.edu/biol_etds/31

2. Hasbay, Utku. "From Single Chemicals to Complex Mixtures": Effect of Contaminants of Emerging Concern on Three Life Stages of Pimephales Promelas.

Degree: Biological Sciences - Cell and Molecular: M.S., Biology, 2019, St. Cloud State University

Aquatic species are exposed to a diverse class of contaminants of emerging concerns (CECs) throughout different life stages. In this study, the effects of CECs in increasing complexity on three life stages of fathead minnows (Pimephales promelas) were assessed using existing Great Lakes tributaries’ chemical occurrence and concentration data. Fathead minnows were exposed to either a water solvent control, or the following chemicals: 4-nonylphenol (surfactant), 5-methyl-1H-benzotriazole (corrosion inhibitor, anti-freezing agent), atrazine (herbicide), bisphenol-a (plasticizer), desvenlafaxine (antidepressant), fexofenadine (allergy medication), estrone (hormonal medication), metformin (antidiabetic medication), metolachlor (herbicide), N,N-diethyl-m-toluamide (insect repellent), sulfamethoxazole (antibiotic), tris(2-butoxyethyl) phosphate (flame retardant), fluoranthene (byproduct of organic raw material pyrolysis), imidacloprid (insecticide), triclosan (antibacterial), ibuprofen (anti-inflammatory medication), 17-beta estradiol (hormonal medication), 2,4,6-trinitrotoluene (energetic), 2,4-dinitroanisole (energetic), 2,6-diamino-3,5-dinitropyrazine-1-oxide (energetic), 2,4,6-trinitro-3-bromoanisole (energetic). All chemicals were used in exposures singly and in mixtures of different complexity. Concentration series for the exposures was centered on medium concentration which contained the highest environmentally measured concentrations while low exposure used 1/10th, ultra-low 1/100th and the high exposure was set to 10x the medium exposure concentration. Adult and larval exposures were conducted simultaneously, while embryonic exposures were conducted at a later time using the same exposure waters. The apical endpoints for the study were survival, overall health, and several reproductive behaviors for adult fathead minnows. Survival, and feeding efficiency data were collected for larval fathead minnows. Lastly, time-to-hatch, and developmental abnormality for fathead minnow embryos were also recorded. Results suggest that the 96 hours CEC exposures affect different apical endpoints depending on the exposed life stage. As the complexity of the chemical exposure increased, alterations in endpoints such as courtship behavior in adult fathead minnows became more frequent. Medium and high concentrations elicited the greatest effects. In both single chemical and mixture exposures, concentration-dependent responses were not observed. This study highlights the need for complementary studies at different exposure time points as well as in vivo studies to identify potential “biological fingerprints” of single chemical effects in complex mixtures. Advisors/Committee Members: Heiko L. Schoenfuss, Satomi Kohno, Nathan Bruender.

Subjects/Keywords: Complex mixtures; Fathead minnow; Contaminant of Emerging Concern; Short-term exposure

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hasbay, U. (2019). "From Single Chemicals to Complex Mixtures": Effect of Contaminants of Emerging Concern on Three Life Stages of Pimephales Promelas. (Masters Thesis). St. Cloud State University. Retrieved from https://repository.stcloudstate.edu/biol_etds/41

Chicago Manual of Style (16th Edition):

Hasbay, Utku. “"From Single Chemicals to Complex Mixtures": Effect of Contaminants of Emerging Concern on Three Life Stages of Pimephales Promelas.” 2019. Masters Thesis, St. Cloud State University. Accessed January 19, 2021. https://repository.stcloudstate.edu/biol_etds/41.

MLA Handbook (7th Edition):

Hasbay, Utku. “"From Single Chemicals to Complex Mixtures": Effect of Contaminants of Emerging Concern on Three Life Stages of Pimephales Promelas.” 2019. Web. 19 Jan 2021.

Vancouver:

Hasbay U. "From Single Chemicals to Complex Mixtures": Effect of Contaminants of Emerging Concern on Three Life Stages of Pimephales Promelas. [Internet] [Masters thesis]. St. Cloud State University; 2019. [cited 2021 Jan 19]. Available from: https://repository.stcloudstate.edu/biol_etds/41.

Council of Science Editors:

Hasbay U. "From Single Chemicals to Complex Mixtures": Effect of Contaminants of Emerging Concern on Three Life Stages of Pimephales Promelas. [Masters Thesis]. St. Cloud State University; 2019. Available from: https://repository.stcloudstate.edu/biol_etds/41

3. Cipoletti, Nicholas. Complex Agricultural Mixtures: Assessing Effects on Aquatic Species (Pimephales promelas and Lepomis spp.) through Short-Term Field and Multi-Generational Laboratory Exposures.

Degree: Biological Sciences - Ecology and Natural Resources: M.S., Biology, 2018, St. Cloud State University

In the aquatic environment, organisms are exposed to complex chemical mixtures throughout life, producing effects not anticipated in laboratory settings designed to test acute exposures of single chemicals. Exposure to chemical mixtures often produces results either not observed or counterintuitive to single chemical exposures. By employing field and laboratory-based exposures using fathead minnows and sunfish, exposures included sensitive life stages otherwise unobserved in adult acute exposure experiments. Field-based studies exposed fathead minnows and sunfish to water collected from sites along the Maumee River (Toledo, OH) to determine the impacts of a land use gradient (upstream – agriculture to downstream – industry and urban). Adult minnows were analyzed for fecundity, physiology, and hematological characteristics (vitellogenin, glucose, 11-keto testosterone, estradiol). Larval minnows were analyzed for growth, predator-avoidance behavior, feeding efficiency, and survival. Embryonic minnows were analyzed for viability, deformities, and time to hatch. Sunfish were either deployed at river sites or resident sunfish were collected for physiology, hematological characteristics (vitellogenin, glucose), and histological analysis. Results of these experiments demonstrate the effect of changing land use on aquatic organisms including reductions and delays in fecundity, alterations to metabolic indices, and greater severity of biological responses of fish exposed to waters from urban settings. Laboratory exposures analyzed the effects of eight co-occurring chemicals found in the Great Lakes watershed in areas of agricultural land use. Fathead minnows were exposed to a complex mixture of environmentally measured concentrations over three generations in a flow-through exposure system to assess the potential physiological, organism, and population level effects. Adult minnows were analyzed for physiology, hematological characteristics (vitellogenin, glucose), behavior, and fecundity. Larval fish were analyzed for growth, predator-avoidance behavior, and feeding efficiency. Juvenile fish were analyzed for growth. Adult minnows demonstrated reductions in fecundity at environmental concentrations in the second exposure generation. In addition, adults demonstrated increasing plasma vitellogenin and glucose, highlighting potential improper direction of energy. Larval and juvenile minnows were unaffected by mixture exposure. The alterations in both field and laboratory settings indicate the extent to which environmentally relevant agricultural mixtures have the potential to pose threats to aquatic organisms through reductions in fecundity, alterations to plasma proteins, and changes in physiology associated with contaminant exposure. Further, the use of a laboratory study demonstrates the need for complete life-cycle assessment of contaminants as indicated by differences between first and second-generation responses. Agricultural practices, and associated aquatic pollution, pose a threat to both the organism and population… Advisors/Committee Members: Heiko Schoenfuss, Satomi Kohno, Nathan Bruender.

Subjects/Keywords: Agriculture; Contaminant Mixtures; Fathead Minnow; Sunfish

…exposure protocols were approved by the St. Cloud State University Institutional Animal Care and… …Laboratory at St. Cloud State University (St. Cloud, MN, USA) using 30L grab water… 

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cipoletti, N. (2018). Complex Agricultural Mixtures: Assessing Effects on Aquatic Species (Pimephales promelas and Lepomis spp.) through Short-Term Field and Multi-Generational Laboratory Exposures. (Masters Thesis). St. Cloud State University. Retrieved from https://repository.stcloudstate.edu/biol_etds/33

Chicago Manual of Style (16th Edition):

Cipoletti, Nicholas. “Complex Agricultural Mixtures: Assessing Effects on Aquatic Species (Pimephales promelas and Lepomis spp.) through Short-Term Field and Multi-Generational Laboratory Exposures.” 2018. Masters Thesis, St. Cloud State University. Accessed January 19, 2021. https://repository.stcloudstate.edu/biol_etds/33.

MLA Handbook (7th Edition):

Cipoletti, Nicholas. “Complex Agricultural Mixtures: Assessing Effects on Aquatic Species (Pimephales promelas and Lepomis spp.) through Short-Term Field and Multi-Generational Laboratory Exposures.” 2018. Web. 19 Jan 2021.

Vancouver:

Cipoletti N. Complex Agricultural Mixtures: Assessing Effects on Aquatic Species (Pimephales promelas and Lepomis spp.) through Short-Term Field and Multi-Generational Laboratory Exposures. [Internet] [Masters thesis]. St. Cloud State University; 2018. [cited 2021 Jan 19]. Available from: https://repository.stcloudstate.edu/biol_etds/33.

Council of Science Editors:

Cipoletti N. Complex Agricultural Mixtures: Assessing Effects on Aquatic Species (Pimephales promelas and Lepomis spp.) through Short-Term Field and Multi-Generational Laboratory Exposures. [Masters Thesis]. St. Cloud State University; 2018. Available from: https://repository.stcloudstate.edu/biol_etds/33

.