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You searched for +publisher:"Rutgers University" +contributor:("White, Eileen P"). Showing records 1 – 2 of 2 total matches.

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1. Pasternack, Robert M, 1985-. Developing a time-dynamic, label-free assay for apoptosis based on optical Gabor filtering.

Degree: PhD, Biomedical Engineering, 2011, Rutgers University

Apoptosis is a fundamental process in the homeostasis and development of mammals, dysfunction of its regulation is implicated in human disease, and it is of growing interest as a possible therapeutic target (especially in cancer). An apoptosis assay that is sensitive, quantitative, and label-free would therefore be useful in cancer drug discovery by offering a direct, time-dynamic in-situ assessment of apoptotic response to drug candidates. This thesis documents the development of optical Gabor filtering into an apoptosis assay. Optical Gabor filtering was developed as an improvement to optical scatter imaging (OSI) capable of optical scatter-based detection of object size and aspect ratio without the need for a predictive scatter model or extrinsic labeling. Two versions of the optical Gabor filtering instrument (LCD-based and DMD-based). By applying a series of Gabor filters with varying spatial frequencies and orientations, a series of filtered images can be generated and processed to extract morphological parameters of interest. The parameters of size, orientedness and aspect ratio are developed and tested using polystyrene microspheres, marine diatoms and collagen gelation, respectively. Orientedness and aspect ratio are employed as morphometric parameters to study dynamic morphological alterations in apoptosis. Aspect ratio measurements in apoptotic iBMK cells show a differential aspect ratio response to apoptosis induction by bax/bak expressing and bax/bak knockout cell variants. To determine the biological source of these variations, multimodal image acquisition of fluorescently-labeled mitochondria alongside Gabor filtered data is used in apoptotic endothelial cells. Results of this study show a drop in orientedness 1-2 h post-STS treatment in mitochondria-rich regions (reconnoitered by fluorescence) concomitant with mitochondrial fragmentation but absent elsewhere. This dynamic is similar but not identical to that observed from iBMK cells. OSI imaging of apoptotic BAEC also revealed that a previously reported drop in optical scatter image ratio in the first hour of apoptosis is also associated with mitochondria-rich regions. In the future, the differences between optical responses of BAEC and iBMK apoptotic cells will be elucidated with fluorescent tracking of mitochondria-rich areas in iBMK. Classification and other computational methods could also be employed to directly evaluate the optical Gabor-filtered data in the future.

Advisors/Committee Members: Pasternack, Robert M, 1985- (author), Boustany, Nada N (chair), Yarmush, Martin l (internal member), Metaxas, Dimitris N (internal member), White, Eileen P (outside member).

Subjects/Keywords: Apoptosis; Optoelectronic devices

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pasternack, Robert M, 1. (2011). Developing a time-dynamic, label-free assay for apoptosis based on optical Gabor filtering. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061437

Chicago Manual of Style (16th Edition):

Pasternack, Robert M, 1985-. “Developing a time-dynamic, label-free assay for apoptosis based on optical Gabor filtering.” 2011. Doctoral Dissertation, Rutgers University. Accessed July 14, 2020. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061437.

MLA Handbook (7th Edition):

Pasternack, Robert M, 1985-. “Developing a time-dynamic, label-free assay for apoptosis based on optical Gabor filtering.” 2011. Web. 14 Jul 2020.

Vancouver:

Pasternack, Robert M 1. Developing a time-dynamic, label-free assay for apoptosis based on optical Gabor filtering. [Internet] [Doctoral dissertation]. Rutgers University; 2011. [cited 2020 Jul 14]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061437.

Council of Science Editors:

Pasternack, Robert M 1. Developing a time-dynamic, label-free assay for apoptosis based on optical Gabor filtering. [Doctoral Dissertation]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061437


Rutgers University

2. Bray, Kevin, 1982-. Manipulation of cell death pathways in cancer.

Degree: Cell and Developmental Biology, 2011, Rutgers University

Subjects/Keywords: Cell death; Cancer—Treatment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bray, Kevin, 1. (2011). Manipulation of cell death pathways in cancer. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bray, Kevin, 1982-. “Manipulation of cell death pathways in cancer.” 2011. Thesis, Rutgers University. Accessed July 14, 2020. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bray, Kevin, 1982-. “Manipulation of cell death pathways in cancer.” 2011. Web. 14 Jul 2020.

Vancouver:

Bray, Kevin 1. Manipulation of cell death pathways in cancer. [Internet] [Thesis]. Rutgers University; 2011. [cited 2020 Jul 14]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063340.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bray, Kevin 1. Manipulation of cell death pathways in cancer. [Thesis]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063340

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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