+publisher:"Rutgers University" +contributor:("Moghe, Prabhas")

Rutgers University

1. Zevon, Margot Alexandra Nash, 1987-. SWIR emitting rare earth albumin nanocomposites for targeted imaging, molecular phenotyping, and improved drug delivery to tumors.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51523/

► Early detection and effective drug delivery remain unresolved challenges that limit the effectiveness of therapeutic regimens against a variety of cancers. Particularly acute are the… (more)

▼ Early detection and effective drug delivery remain unresolved challenges that limit the effectiveness of therapeutic regimens against a variety of cancers. Particularly acute are the challenges associated with penetration into the dense matrix of solid tumors and the targeting of metastatic lesions before they become unmanageable. Current clinical imaging techniques employed to detect these lesions are only able to provide anatomical macroscopic information on a tumor state. Additionally, many of these modalities lack the resolution to detect microlesions at a stage ideal for therapeutic intervention, and are unable to resolve lesions in specific tissues such as bone. The early detection and sensitive tracking of disease states is critical to successful management and treatment. While imaging modalities such as MRI and ultrasound provide only anatomical details, optical imaging techniques can provide high content, high resolution images detailing the location and molecular phenotype of a tumor. This is critical in the management of many malignancies including breast cancer, as cell receptor expression dictates therapeutic regimen and is often not conserved through disease progression. Despite the potential for optical imaging to fill this critical role in breast cancer management, there are still numerous challenges that limit its clinical translation. Rare earth nanoprobes (ReNPs) are bright, stable, optically efficient contrast agents that provide many unique advantages over traditional optical imaging fluorophores. When excited with near infrared (NIR, 700-1000 nm) photons, ReNPs emit fluorescence in both the visible (400-700 nm) and short wave infrared (SWIR, 1000-3000 nm) ranges. While the “upconverted” visible photons are quickly absorbed or scattered by biological tissue, SWIR photons can more easily penetrate tissue and be detected via specialized cameras and sensors. In order to impart solubility and cytocompatability, we have encapsulated ReNPs in human serum albumin to generate rare earth albumin nanocomposites (ReANCs). The tunable albumin coating of ReANCs allows for a wide range of ligands, therapeutic payloads, and permeation enhancers to be conjugated to the surface of the particle’s shell. These characteristics yield multifunctional nanoprobes that can be adapted for many purposes including targeted imaging. This dissertation is focused on engineering biocompatible ReANCs as a multifunctional contrast agent capable of improved payload delivery, in vivo SWIR based optical imaging, and targeted imaging and molecular mapping of solid tumors. This study describes the development of drug loaded, tumor penetrating albumin nanoshells designed to overcome physiological barriers to solid tumor drug delivery. These nanoparticles are then further engineered to encapsulate SWIR emitting contrast agents, which form the basis of a novel optical imaging platform capable of the sensitive and specific detection of metastatic breast cancer. The resulting ReANCs were targeted to markers of breast cancer metastasis to… Advisors/Committee Members: Moghe, Prabhas V (chair), Roth, Charles M (co-chair).

Subjects/Keywords: Drug delivery systems; Rare earth metal compounds; Nanoparticles

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APA (6^th Edition):

Zevon, Margot Alexandra Nash, 1. (2016). SWIR emitting rare earth albumin nanocomposites for targeted imaging, molecular phenotyping, and improved drug delivery to tumors. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51523/

Chicago Manual of Style (16^th Edition):

Zevon, Margot Alexandra Nash, 1987-. “SWIR emitting rare earth albumin nanocomposites for targeted imaging, molecular phenotyping, and improved drug delivery to tumors.” 2016. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51523/.

MLA Handbook (7^th Edition):

Zevon, Margot Alexandra Nash, 1987-. “SWIR emitting rare earth albumin nanocomposites for targeted imaging, molecular phenotyping, and improved drug delivery to tumors.” 2016. Web. 15 Apr 2021.

Vancouver:

Zevon, Margot Alexandra Nash 1. SWIR emitting rare earth albumin nanocomposites for targeted imaging, molecular phenotyping, and improved drug delivery to tumors. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51523/.

Council of Science Editors:

Zevon, Margot Alexandra Nash 1. SWIR emitting rare earth albumin nanocomposites for targeted imaging, molecular phenotyping, and improved drug delivery to tumors. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51523/

Rutgers University

2. Mishra, Prakhar, 1987-. Fluorescence imaging based analysis of actin turnover: longitudinal profiling of stem cell phenotypes during differentiation.

Degree: PhD, Mesenchymal stem cells, 2020, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/64962/

► Stem cells derived from adults have become the focal point of cell-based regenerative therapies. The most commonly used stem cells, mesenchymal stem cells (abbreviated MSCs),… (more)

▼ Stem cells derived from adults have become the focal point of cell-based regenerative therapies. The most commonly used stem cells, mesenchymal stem cells (abbreviated MSCs), have several traits such as the ability to migrate to injury site, immunomodulation, in vitro expansion and differentiation into a variety of cells. However, the widespread usage of MSCs is hampered by poor characterization, tissue culture induced senescence and innate heterogeneity that makes it challenging to predict their clinical efficacy. The traditional methods for evaluation of stem cells are primarily terminal assays that rely on discrete time-point data sets that offer limited, discontinuous information and often overlook the intrinsic phenotypic diversity of these cells. In this thesis, we address some of these issues by developing a novel platform for live cell imaging which can be used to monitor evolving MSCs by generating continuous data sets over the course of several hours or several days while sparing the cells for multiplexing with other traditional assays. Our imaging-based approach relies on a cell permeable fluorogenic probe, SiR-actin (SA), that becomes fluorescent only when it labels endogenous actin filaments (F-actin). The idea of harnessing actin as a sentinel stem cell reporter is supported from past research conducted by Moghe lab and numerous other research groups, as the cytoskeleton can modulate and display the phenotypic changes associated with lineage commitment. Unlike previous research that focused primarily on morphology of actin cytoskeleton, we focused on utilizing the shifting actin turnover rates in response to extracellular cues as the key reporter metric. After SA labelling, actin reorganization leads to removal of the SA probe from F-actin binding sites and subsequent decline in SA fluorescence. When cells are cultured in differentiation media, the rate of fluorescence loss of SA provided insights about the kinetics of lineage specific change in actin reorganization. We report that initiation of differentiation involves decline in actin turnover during adipogenesis and chondrogenesis within few hours. By combining SA with another F-actin probe, phalloidin, we were able to parse heterogenous single cells across the standard tri-lineages (adipogenic, osteogenic and chondrogenic) within 1 hour of stimulation. In addition, our approach enabled assessment of in vitro aging by demonstrating a slowdown in actin turnover within 1 hour of analysis. Next, to establish the link between actin turnover and stem cell differentiation, we employed immunolabeling to demonstrate co-occurrence of altered actin turnover with differentiation markers in MSCs as well induced pluripotent stem cells (iPSCs). We also propose that inherent actin turnover status of individual cells could be a determinant of their differentiation potential. To this end, we isolated cells based on differential SA expression and found that the actin turnover plays a role in determining the ability to differentiate towards osteogenic or adipogenic… Advisors/Committee Members: Moghe, Prabhas (chair), School of Graduate Studies.

Subjects/Keywords: Cell and Developmental Biology

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APA (6^th Edition):

Mishra, Prakhar, 1. (2020). Fluorescence imaging based analysis of actin turnover: longitudinal profiling of stem cell phenotypes during differentiation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/64962/

Chicago Manual of Style (16^th Edition):

Mishra, Prakhar, 1987-. “Fluorescence imaging based analysis of actin turnover: longitudinal profiling of stem cell phenotypes during differentiation.” 2020. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/64962/.

MLA Handbook (7^th Edition):

Mishra, Prakhar, 1987-. “Fluorescence imaging based analysis of actin turnover: longitudinal profiling of stem cell phenotypes during differentiation.” 2020. Web. 15 Apr 2021.

Vancouver:

Mishra, Prakhar 1. Fluorescence imaging based analysis of actin turnover: longitudinal profiling of stem cell phenotypes during differentiation. [Internet] [Doctoral dissertation]. Rutgers University; 2020. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/64962/.

Council of Science Editors:

Mishra, Prakhar 1. Fluorescence imaging based analysis of actin turnover: longitudinal profiling of stem cell phenotypes during differentiation. [Doctoral Dissertation]. Rutgers University; 2020. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/64962/

Rutgers University

3. Bennett, Neal, 1989-. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/

►

Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by motor dysfunction, eventual cognitive impairment and dementia in advanced stages. These symptoms arise as a… (more)

▼

Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by motor dysfunction, eventual cognitive impairment and dementia in advanced stages. These symptoms arise as a result of decreased activity or death of dopaminergic (DA) neurons in the substantia nigra region of the brain, leading to dopamine depletion in the striatum disruption of neuronal circuitry in the basal ganglia. The current most common treatment for PD is levodopa, which can be converted into dopamine by surviving DA neurons, offering temporary relief of the motor dysfunction symptoms of PD. However, a key disadvantage of levodopa as a therapeutic strategy is that it does little to address the progression of PD and symptoms typically worsen as DA neurons continue to degenerate or die. Based on the a critical need for more comprehensive therapeutic approaches to PD that do more than relieve dopamine deficiency, but also disrupt the factors causing disease progression, the work in this dissertation focus on our efforts to address key aspects of Parkinson's disease pathology: neuronal degeneration, neuroinflammation, and synucleinopathy. To address neuronal degeneration, we investigated the potential for 3D fibrous synthetic substrates to support and transplant populations of human reprogrammed neurons into the brain. We found that fibrous substrate geometries could be tuned to shift reprogrammed cell populations towards either neuronal differentiation or maintenance of pluripotency. Microscale scaffolds generated from these fibrous substrates improved transplanted neuronal survival by at least an order of magnitude over traditional cell transplantation techniques. These proof-of-concept studies could be used to inform the future design of transplantable scaffolds supporting neurons reprogrammed to best address DA deficiency in PD. To address neuroinflammation and synucleinopathy, we examined the potential of microglia-targeting nanotherapeutics. We first identified scavenger receptors as a microglial receptor for α-synuclein (ASYN), a protein that forms characteristic protein aggregates and activates microglia in PD. We then designed nanoparticles targeting this interaction using synthetic amphiphilic scavenger receptor ligands. These amphiphilic molecules could reduce ASYN internalization and intracellular aggregation by microglia. We used nanoparticle constructs made using these synthetic ligands to target delivery of antioxidants to microglia, decreasing microglial activation in response to aggregated ASYN in vitro and in vivo. In summary, the studies described in this dissertation establish a valuable foundation for future therapeutic strategies addressing key features of PD pathophysiology and progression.

Advisors/Committee Members: Moghe, Prabhas V (chair), Pang, Zhiping (internal member), Grumet, Martin (internal member), Baum, Jean (outside member).

Subjects/Keywords: Parkinson's disease – Treatment; Nanobiotechnology

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APA (6^th Edition):

Bennett, Neal, 1. (2016). Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51233/

Chicago Manual of Style (16^th Edition):

Bennett, Neal, 1989-. “Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.” 2016. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51233/.

MLA Handbook (7^th Edition):

Bennett, Neal, 1989-. “Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.” 2016. Web. 15 Apr 2021.

Vancouver:

Bennett, Neal 1. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/.

Council of Science Editors:

Bennett, Neal 1. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/

Rutgers University

4. Chen, Ho-Chung, 1991-. Expression of mutated BRAF (V600E) in melanocytes activates metabotropic glutamate receptor 1 expression.

Degree: MS, Chemical and Biochemical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51246/

► Melanoma is the most aggressive form of skin cancer, which is caused by the neoplastic transformation of melanocytes. The precise genetic aberrations that initiate the… (more)

▼ Melanoma is the most aggressive form of skin cancer, which is caused by the neoplastic transformation of melanocytes. The precise genetic aberrations that initiate the transformation process remain largely unknown. Several genetic mutations have shown to be critical in maintaining the transformed phenotypes and the temporal expression of these mutated proteins is also vital on the onset of dysregulated cell growth. The current study is to explore a relationship between two genetic aberrations, both have been shown to play a role in melanoma development. Our group has identified the ectopic expression of a normal neuronal receptor, metabotropic glutamate receptor 1 (Grm1) in melanocytes was sufficient to induce spontaneous metastatic melanoma development in vivo by the establishment of two transgenic mouse models (TG-3 and Epv). Mutated BRAF at residues 600 was shown in about 60% of nevi and melanoma specimens. Mouse models with mutated BRAF alone led to hyper-proliferated melanocytes in the absence of tumor development. Unexpectedly, we detected Grm1 expression in tumor samples harboring mutated B-RAF(V600E) mutation on PTEN null background by immunohistochemical staining. We hypothesize that one of the consequences of mutated BRAFis activation of Grm1 expression. We took advantage of an inducible BRAFmouse model, BJB, which has an inducible BRAF mutation (V600E) genotype only in melanocytes. We observed hyperpigmentation on their ears, tails, and skin samples, Grm1 expression was also detected. There was no tumor formation even after 13 months, while Grm1 expression persisted. We hypothesize that the order of the expression of mutated BRAF and Grm1 must play a role in the onset of melanocytic transformation. In in vitro cumltured cell experiments, we also detected Grm1 expression after introduction of mutated BRAF into immortalized non-tumorigenic mouse melanocytes, MelanA cells. Moreover, siRNA decrease in mutated BRAF expression by silencing RNA resulted in parallel reduction in Grm1 expression. Taken together, these results point to an association between mutated BRAF and Grm1 expression in vivo and in vitro. Advisors/Committee Members: Chen, Suzie (chair), Roth, Charles M. (co-chair), Moghe, Prabhas V. (internal member).

Subjects/Keywords: Melanoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, Ho-Chung, 1. (2016). Expression of mutated BRAF (V600E) in melanocytes activates metabotropic glutamate receptor 1 expression. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51246/

Chicago Manual of Style (16^th Edition):

Chen, Ho-Chung, 1991-. “Expression of mutated BRAF (V600E) in melanocytes activates metabotropic glutamate receptor 1 expression.” 2016. Masters Thesis, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51246/.

MLA Handbook (7^th Edition):

Chen, Ho-Chung, 1991-. “Expression of mutated BRAF (V600E) in melanocytes activates metabotropic glutamate receptor 1 expression.” 2016. Web. 15 Apr 2021.

Vancouver:

Chen, Ho-Chung 1. Expression of mutated BRAF (V600E) in melanocytes activates metabotropic glutamate receptor 1 expression. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51246/.

Council of Science Editors:

Chen, Ho-Chung 1. Expression of mutated BRAF (V600E) in melanocytes activates metabotropic glutamate receptor 1 expression. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51246/

Rutgers University

5. Turner, Jeffrey Thomas, 1989-. Human neural stem cell differentiation and electrical stimulation on a novel single walled carbon nanotube-polymer composite.

Degree: MS, Biomedical Engineering, 2013, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/41938/

► Increasing control of human neural stem cell (hNSC) differentiation is critical to development of cellular models for neurodegenerative diseases such as Parkinson’s disease because current… (more)

▼ Increasing control of human neural stem cell (hNSC) differentiation is critical to development of cellular models for neurodegenerative diseases such as Parkinson’s disease because current methods do not result in the required fully developed cells. In addition, existing cell culture and differentiation regimen are inefficient due to lengthy differentiation times and low yields of functional cells. The use of carbon nanotubes (CNTs), particularly in 3D geometries, offers a possible solution by improving the kinetics and efficiency of NSC differentiation. Electrical stimulation through conductive substrates, such as CNTs, can cause increased rates of NSC differentiation. In this work, a combination of a three dimensional, in vivo mimetic, single walled CNT substrate and electrical stimulation is used to investigate survival and differentiation behaviors of hNSCs derived from induced pluripotent stem cells (iPSCs). First, fibrous poly(lactic-co-glycolic acid) (PLGA) substrates, with an average fiber diameter of 1.11μm, are manufactured via electrospinning onto a flat plate collector. A novel vacuum driven impregnation technique forces an aqueous dispersion of CNTs to coat the PLGA fibers while maintaining the microscale features of the fibers’ architecture. The CNTs provide increased electrical conductivity, >0.1 S/m up to 25 S/m, and nanosurface roughness, which can increase neurite interfacial interactions, resulting in improved differentiation of NSCs to neurons. Immunocytochemistry of hNSC differentiated on these surfaces reveal an 18% rise in the number of cells staining positive for neurofilament M (NFM), a marker of maturing neurons, on CNT versus control PLGA substrate after 14 days of differentiation. When a 10 minute, 30μA direct current stimulation is applied on the 3rd day of differentiation, there is a further 4% improvement in the number of cells staining positive for NFM on the 14th day of differentiation. Calcium imaging indicates that on day 14, 0.3% of cells on PLGA scaffolds compared to 5.9% of cells on the CNT composite substrate had an electrical event in response to electrical stimulation. These results strongly support the use of electrically conductive CNT substrates for neural differentiation and suggest electrical cues could be more systematically investigated for directing the differentiation process to sub-type specific and functional human neuronal systems. Advisors/Committee Members: Moghe, Prabhas V (chair), Neimark, Alex V (internal member), Shreiber, David I (internal member).

Subjects/Keywords: Neural stem cells – Differentiation; Neural stem cells; Nanotubes

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APA (6^th Edition):

Turner, Jeffrey Thomas, 1. (2013). Human neural stem cell differentiation and electrical stimulation on a novel single walled carbon nanotube-polymer composite. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/41938/

Chicago Manual of Style (16^th Edition):

Turner, Jeffrey Thomas, 1989-. “Human neural stem cell differentiation and electrical stimulation on a novel single walled carbon nanotube-polymer composite.” 2013. Masters Thesis, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/41938/.

MLA Handbook (7^th Edition):

Turner, Jeffrey Thomas, 1989-. “Human neural stem cell differentiation and electrical stimulation on a novel single walled carbon nanotube-polymer composite.” 2013. Web. 15 Apr 2021.

Vancouver:

Turner, Jeffrey Thomas 1. Human neural stem cell differentiation and electrical stimulation on a novel single walled carbon nanotube-polymer composite. [Internet] [Masters thesis]. Rutgers University; 2013. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41938/.

Council of Science Editors:

Turner, Jeffrey Thomas 1. Human neural stem cell differentiation and electrical stimulation on a novel single walled carbon nanotube-polymer composite. [Masters Thesis]. Rutgers University; 2013. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41938/

Rutgers University

6. Vega, Sebastian, 1984-. Single cell organizational imaging of cytoskeletal and nuclear proteins: correlational studies and classification of stem cell states.

Degree: PhD, Chemical and Biochemical Engineering, 2015, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/46448/

►

Two major challenges plague the robust design of stem cell-derived tissues for regenerative therapies: (1) the phenotypic and functional heterogeneity inherent in stem cell cultures,… (more)

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Two major challenges plague the robust design of stem cell-derived tissues for regenerative therapies: (1) the phenotypic and functional heterogeneity inherent in stem cell cultures, and (2) the dynamic, long-term nature of stem cell responses to microenvironmental cues. Several tools have emerged to precisely characterize how stem cells respond to various stimuli and scaffold properties; however, these tools are limiting because they are population-based and rely on the detection of markers expressed in fully differentiated cells. Thus, methods to characterize individual stem cells from a population is key for establishing cell-biomaterial relationships necessary to design scalable constructs for tissue engineering applications. This thesis dissertation focuses on the utility of single cell profiling techniques to identify the heterogeneity within cell cultures and characterize responses to controllable changes in diverse microenvironments. This method relies on the quantification of metrics derived from images of cytoskeletal and nuclear proteins that are sensitive to microenvironmental cues that influence cell state. This is achieved by pursuing two thesis-specific aims: (1) to utilize single cell biological imaging and machine learning techniques to identify cell subtypes in heterogeneous cultures, and (2) to use early morphological descriptors of intranuclear mechanotransductive proteins to predict long-term stem cell responses to biomaterials. In this study we report that single cell imaging-based profiling of cytoskeletal actin and nuclear mitotic apparatus (NuMA), a cell cycle regulating protein, can identify different cell phenotypes in heterogeneous stem cell cultures, progenitor cells derived from different tissue sections ex vivo, and stem cell responses to a diverse set of surface chemistries. We also show that the early (3 day) organization of interchromatin domains varies in human mesenchymal stem cells exposed to a variety of growth factor combinations and complex topographical microenvironments that induce long-term (> 7 day) divergent phenotypic outcomes. In summary, the results presented in this thesis dissertation show that single cell imaging-based profiling can be utilized to identify cell subtypes and predict microenvironment-induced differentiation fates at earlier times and with more resolution than current screening assays. This work can help lay the foundation for a new generation of single cell-based biomaterial screening tools and cellular phenotyping techniques.

Advisors/Committee Members: Moghe, Prabhas V. (chair), Kohn, Joachim (co-chair), Androulakis, Ioannis (internal member), Serrano, Lourdes (outside member).

Subjects/Keywords: Stem cells; Single cell proteins

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APA (6^th Edition):

Vega, Sebastian, 1. (2015). Single cell organizational imaging of cytoskeletal and nuclear proteins: correlational studies and classification of stem cell states. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/46448/

Chicago Manual of Style (16^th Edition):

Vega, Sebastian, 1984-. “Single cell organizational imaging of cytoskeletal and nuclear proteins: correlational studies and classification of stem cell states.” 2015. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/46448/.

MLA Handbook (7^th Edition):

Vega, Sebastian, 1984-. “Single cell organizational imaging of cytoskeletal and nuclear proteins: correlational studies and classification of stem cell states.” 2015. Web. 15 Apr 2021.

Vancouver:

Vega, Sebastian 1. Single cell organizational imaging of cytoskeletal and nuclear proteins: correlational studies and classification of stem cell states. [Internet] [Doctoral dissertation]. Rutgers University; 2015. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46448/.

Council of Science Editors:

Vega, Sebastian 1. Single cell organizational imaging of cytoskeletal and nuclear proteins: correlational studies and classification of stem cell states. [Doctoral Dissertation]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46448/

Rutgers University

7. Kim, Joseph Jung-Woong, 1983-. Single cell high content textural image analysis of dynamic epigenetic signatures as a response to defined microenvironmental parameters.

Degree: PhD, Biomedical Engineering, 2015, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/46370/

►

Currently, quantifiable investigations of the epigenome require cell lysis and are population based, prohibiting direct investigations of intact intranuclear structural organization and introducing noise into… (more)

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Currently, quantifiable investigations of the epigenome require cell lysis and are population based, prohibiting direct investigations of intact intranuclear structural organization and introducing noise into data obtained from inherently heterogeneous stem cell populations. To address this, we have developed and employed a single-cell high-content image informatics framework to capture organizational signatures of epigenetic signaling components from images of cellular nuclei obtained via superresolution nanoscopy. High dimensional quantitative texture descriptors of the organizational dynamics of key posttranslational modifications to core histone proteins were imaged in different human stem cell systems using time-gated stimulated emission depletion confocal nanoscopy. Influential texture descriptors were identified, validated at the nanoscale using immuno-gold electron microscopy, and organizational sub-classifiers were generated from this bioimage informatics data representing a range of “open” versus “closed” chromatin states. When applied to growth factor-induced lineage differentiation of human mesenchymal stem cells, the organizational classifiers showed a clear evolution with temporal cell state, which was more sensitive than the conventional mass spectrometry-based quantitation of the relative abundance of these PTMs. When a range of stem cell phenotypes sharing common DNA sequences were imaged, clear sub-classifiers emerged correlating with the divergent phenotypes for undifferentiated, adipogenic, and osteogenic hMSCs, as well as for human foreskin fibroblasts, induced pluripotent stem cells, neural stem cells, and reprogrammed neurons. Thus, high content bioimage informatics reflective of chromatin organization yields a higher order organizational signature corresponding to an epigenetic “activity” state. To elucidate the influence of biophysical factors on stem cell epigenetic states, these imaging-based organizational classifiers were tested on human mesenchymal stem cells exposed to physically constraining cues, and successfully predicted the early differentiation toward adipogenic hMSCs on hydrogel substrates with spatially graded mechanical stiffness, as well as osteogenic hMSCs on soft-lithographed, graded nanotopographies. In summary, in contrast to the traditional reductionist, population-level readouts in epigenomics, the approach outlined in this thesis offers a more integrated, single-cell, organizational index of emergent stem cell activity in response to defined environmental cues, and can be applied for the screening of discrete microenvironmental properties for the enhancement of stem cell behavioral control and facilitated integration in regenerative medicine applications.

Advisors/Committee Members: Moghe, Prabhas (chair), Verzi, Michael (internal member), Androulakis, Ioannis (internal member), Boustany, Nada (internal member), Serrano, Lourdes (internal member), Madabhushi, Anant (outside member).

Subjects/Keywords: Epigenetics; Image processing – Data processing; Electron microscopy

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APA (6^th Edition):

Kim, Joseph Jung-Woong, 1. (2015). Single cell high content textural image analysis of dynamic epigenetic signatures as a response to defined microenvironmental parameters. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/46370/

Chicago Manual of Style (16^th Edition):

Kim, Joseph Jung-Woong, 1983-. “Single cell high content textural image analysis of dynamic epigenetic signatures as a response to defined microenvironmental parameters.” 2015. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/46370/.

MLA Handbook (7^th Edition):

Kim, Joseph Jung-Woong, 1983-. “Single cell high content textural image analysis of dynamic epigenetic signatures as a response to defined microenvironmental parameters.” 2015. Web. 15 Apr 2021.

Vancouver:

Kim, Joseph Jung-Woong 1. Single cell high content textural image analysis of dynamic epigenetic signatures as a response to defined microenvironmental parameters. [Internet] [Doctoral dissertation]. Rutgers University; 2015. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46370/.

Council of Science Editors:

Kim, Joseph Jung-Woong 1. Single cell high content textural image analysis of dynamic epigenetic signatures as a response to defined microenvironmental parameters. [Doctoral Dissertation]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46370/

Rutgers University

8. Higgins, Laura, 1989-. Development and testing of multi-modal, multi-scale imaging systems for rare-earth-doped contrast agents.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51309/

► Rare-earth based nanoparticles have appealing properties for use as contrast agents in biomedical imaging. With unique luminescent properties and a refractive index that is higher… (more)

▼ Rare-earth based nanoparticles have appealing properties for use as contrast agents in biomedical imaging. With unique luminescent properties and a refractive index that is higher than that of tissue, these versatile materials have a wide range of potential applications spanning basic science research to preclinical testing and clinical translation. The goal of this dissertation was to exploit the unique optical properties of these materials to develop new multi-modal, multi-scale imaging platforms and expand the applicability of these materials in biomedicine. Under near-infrared excitation, these particles emit conventional Stokes-shifted fluorescence in the short wave infrared region (SWIR), as well as a higher-energy upconversion signal in the visible spectrum. Previous work, focused on wide-field imaging of the SWIR fluorescence, has shown the ability to detect the accumulation of biofunctionalized rare-earth albumin nanocomposites (fREANCs) in preclinical models of cancer metastasis. This dissertation builds upon previous studies, exploring high- resolution imaging of these particles to serve as an â€œoptical biopsyâ€ through the combination of confocal microscopy and optical coherence tomography (OCT). Confocal microscopy provides subcellular resolution imaging from near the tissue surface while OCT is capable of imaging tissue microstructure up to 1-2 mm below the surface, both important factors in the evaluation of disease progression. High-resolution imaging of these materials in thick tissues has been limited by the long emission lifetimes of the rare-earth elements. This work demonstrates that line- scanning confocal microscopy (LSC) can overcome these challenges by extending the excitation and emission time while maintaining frame rate, providing a method for real- time, high-resolution imaging of rare-earth doped contrast agents in ex vivo tissue samples. Although the refractive index mismatch did not increase OCT backscattering in tissue, motivating the development of fREANCs as a molecularly-targeted OCT contrast agent, OCT can still provide valuable insight into tissue disease state based on native optical properties. From this work, a multi-modal imaging platform was developed combining OCT with line-scanning confocal microscopy of fREANCs. A first-generation proof-of-concept system, combining OCT with full-field fluorescence microscopy allowed for the identification of potential technical challenges, before integrating OCT within the line- scanning microscope. Through the combination of LSC and OCT, the second-generation system achieved an en face lateral resolution of 2.8 Î¼m (LSC) with an imaging depth of 1.4 mm (OCT). Finally, this multi-modal imaging platform was evaluated within a preclinical study, designed to explore multi-scale imaging of fREANCs to identify tumors in the lungs. Through this work, signature features of healthy and malignant tissue were identified within the multi-modal image sets, with the potential to serve as a basis for future high-resolution in vivo imaging as a… Advisors/Committee Members: Pierce, Mark C (chair), Moghe, Prabhas V (internal member), Boustany, Nada (internal member), Davidov, Tomer (outside member).

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Higgins, Laura, 1. (2016). Development and testing of multi-modal, multi-scale imaging systems for rare-earth-doped contrast agents. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51309/

Chicago Manual of Style (16^th Edition):

Higgins, Laura, 1989-. “Development and testing of multi-modal, multi-scale imaging systems for rare-earth-doped contrast agents.” 2016. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51309/.

MLA Handbook (7^th Edition):

Higgins, Laura, 1989-. “Development and testing of multi-modal, multi-scale imaging systems for rare-earth-doped contrast agents.” 2016. Web. 15 Apr 2021.

Vancouver:

Higgins, Laura 1. Development and testing of multi-modal, multi-scale imaging systems for rare-earth-doped contrast agents. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51309/.

Council of Science Editors:

Higgins, Laura 1. Development and testing of multi-modal, multi-scale imaging systems for rare-earth-doped contrast agents. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51309/

Rutgers University

9. De Silva Indrasekara, Agampodi Swarnapali, 1983-. Fabrication of surface enhanced raman scattering substrates by controlled assembly and morphology tuning of gold nanoparticles.

Degree: PhD, Materials Science and Engineering, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45228/

►

The design and fabrication of nanoparticles (NPs) and NP assemblies to sustain intense electromagnetic field enhancement for surface enhanced Raman scattering (SERS)-based imaging and sensing… (more)

▼

The design and fabrication of nanoparticles (NPs) and NP assemblies to sustain intense electromagnetic field enhancement for surface enhanced Raman scattering (SERS)-based imaging and sensing have recently gained significant attention. In SERS, the intrinsic optical properties of plasmonic NPs are used to overcome the relatively low Raman cross-sections and thereby increase sensitivity. Sharp features in anisotropic NPs and interparticle gaps within NP assemblies have been identified as the locations where the highest SERS enhancements can be achieved, also known as “hot spots”. Many attempts have been reported that deal with the bottom-up assembly of NPs to achieve highly reproducible, sensitive, and well characterized SERS substrates, but it still remains a challenge to attain monodisperse, highly reproducible “hot spots” and directional assembly. The focus of this dissertation is to develop synthetic protocols for controlled engineering of NPs with SERS “hot spots”, and thereby to contribute to the advancement of SERS-based sensing and imaging applications. In this dissertation, the development of SERS substrates has evolved from dimers of spherical gold NPs (SP), to star-shaped gold NPs (ST), and finally to assembled superstructures of ST and SPs. The kinetically controlled assembly of SPs into dimers was achieved by using Raman active dithiolated linker molecules, with the highest yield reported for this method to the best of our knowledge, leading to SERS tags with a reproducible SERS enhancement on the order of 105. NP dimers, surface-functionalized to target U87 glioblastoma cancer cells, demonstrated a fast, reliable, and selective SERS-based detection of the diseased cells that outperforms fluorescence. The morphology of the STs was modified to possess longer and sharper spikes with a narrower tip curvature thereby increasing the electromagnetic field localization at the tips. SERS substrates were designed by periodically and reproducibly immobilizing STs on a planar substrate with high surface coverage and limited to no clustering, thus enabling femtomolar detection of organic analytes with an outstanding 109 SERS enhancement. Finally, the core-satellite assemblies of ST with SPs were achieved through conjugation linker chemistry. These assemblies demonstrated SERS enhancement of two orders of magnitudes greater than isolated STs thereby improving the sensitivity of potential SERS-based imaging and sensing applications.

Advisors/Committee Members: Fabris, Laura (chair), Moghe, Prabhas V (internal member), Mann, Adrian B (internal member), Arslanoglu, Julie-Anne M (outside member).

Subjects/Keywords: Raman spectroscopy; Surface chemistry; Nanoparticles

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APA (6^th Edition):

De Silva Indrasekara, Agampodi Swarnapali, 1. (2014). Fabrication of surface enhanced raman scattering substrates by controlled assembly and morphology tuning of gold nanoparticles. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45228/

Chicago Manual of Style (16^th Edition):

De Silva Indrasekara, Agampodi Swarnapali, 1983-. “Fabrication of surface enhanced raman scattering substrates by controlled assembly and morphology tuning of gold nanoparticles.” 2014. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/45228/.

MLA Handbook (7^th Edition):

De Silva Indrasekara, Agampodi Swarnapali, 1983-. “Fabrication of surface enhanced raman scattering substrates by controlled assembly and morphology tuning of gold nanoparticles.” 2014. Web. 15 Apr 2021.

Vancouver:

De Silva Indrasekara, Agampodi Swarnapali 1. Fabrication of surface enhanced raman scattering substrates by controlled assembly and morphology tuning of gold nanoparticles. [Internet] [Doctoral dissertation]. Rutgers University; 2014. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45228/.

Council of Science Editors:

De Silva Indrasekara, Agampodi Swarnapali 1. Fabrication of surface enhanced raman scattering substrates by controlled assembly and morphology tuning of gold nanoparticles. [Doctoral Dissertation]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45228/

Rutgers University

10. Bhamidipati, Manjari, 1987-. SERS-based detection and quantification of cancer biomarkers in cells and tissue microarrays.

Degree: PhD, Biomedical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/58898/

► The need for early detection of cancer has resulted in the development of a number of molecular identification techniques that can screen for cancer biomarkers… (more)

▼ The need for early detection of cancer has resulted in the development of a number of molecular identification techniques that can screen for cancer biomarkers or cellular components indicative of a cancerous state. Biomarkers play a crucial role in cancer diagnosis and their identification and quantification can help monitor disease progression and therefore significantly contribute to clinical prognosis and to individualization and optimization of systemic therapy. Nanomaterial-based imaging systems provide the sensitivity, selectivity, and high multiplexing capability needed for molecular detection when leveraging the optical phenomenon of surface-enhanced Raman scattering (SERS). SERS is a vibrational spectroscopic technique that can be used to detect molecules present on or near the surface of plasmonic nanomaterials such as gold nanoparticles. The objective of this dissertation was to develop a SERS-based imaging tool for phenotype assessment of cancer cells and tissues by utilizing the optical properties of gold nanostars (i.e. gold nanoparticles (AuNPs) with a spherical core and sharp protruding spikes) together with the effective targeting ability of aptamers. It was hypothesized that gold nanostars would be able to provide excellent SERS enhancement factors that would enable the quantification of biomarker expression at the single cell level. However, toxicity of the nanoparticles is a critical issue that needs to be taken into consideration before their implementation in vitro. For this purpose, detailed multi-parametric assessment of gold nanoparticle toxicity was carried out in cancerous (glioblastoma cells, U-87) and healthy cells (human fibroblasts) where the effects of nanoparticle shape, surface chemistry, and size on cell toxicity and cellular uptake were assessed. The study demonstrated that gold nanostars can be effectively taken up by both cell types without inducing significant toxicity when capped by a suitable ligand. They were therefore considered to be less toxic when compared to other nanoparticle shapes with the same surface coating and at the same dosage. Gold nanostars were then implemented in the development of a SERS based sensing platform for the recognition of soluble and cell membrane embedded epithelial cell adhesion molecule (EpCAM) protein with high sensitivity and selectivity. EpCAM is a key epithelial biomarker that is overexpressed in several types of cancer and changes in its expression have been associated with the onset of metastasis. The developed biosensor enabled the detection and quantification of EpCAM at the single cell level in two cancer cells with varying expression levels, MCF-7 and PC-3. The highly sensitive cellular targeting with a detection limit of 10 pM was achieved by using EpCAM aptamers. The results demonstrated potential in using this approach for detecting cells that undergo phenotype variations during cancer metastasis. Finally, the gold nanostar aptamer-based SERS tags were implemented to distinguish between early and late stage prostate cancer based… Advisors/Committee Members: Fabris, Laura (chair), Moghe, Prabhas (internal member), Pierce, Mark (internal member), Kim, Isaac (outside member), School of Graduate Studies.

Subjects/Keywords: Raman effect, Surface enhanced; Cancer—Early detection

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APA (6^th Edition):

Bhamidipati, Manjari, 1. (2018). SERS-based detection and quantification of cancer biomarkers in cells and tissue microarrays. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/58898/

Chicago Manual of Style (16^th Edition):

Bhamidipati, Manjari, 1987-. “SERS-based detection and quantification of cancer biomarkers in cells and tissue microarrays.” 2018. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/58898/.

MLA Handbook (7^th Edition):

Bhamidipati, Manjari, 1987-. “SERS-based detection and quantification of cancer biomarkers in cells and tissue microarrays.” 2018. Web. 15 Apr 2021.

Vancouver:

Bhamidipati, Manjari 1. SERS-based detection and quantification of cancer biomarkers in cells and tissue microarrays. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/58898/.

Council of Science Editors:

Bhamidipati, Manjari 1. SERS-based detection and quantification of cancer biomarkers in cells and tissue microarrays. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/58898/

Rutgers University

11. Olekson, Melissa Ann, 1986-. Strategies for improving growth factor function in diabetic wounds.

Degree: PhD, Biomedical Engineering, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45385/

►

Diabetic Foot Ulcers (DFUs) are debilitating non-healing wounds that often lead to amputation. The dermal scaffold is a promising treatment strategy that provides a template… (more)

▼

Diabetic Foot Ulcers (DFUs) are debilitating non-healing wounds that often lead to amputation. The dermal scaffold is a promising treatment strategy that provides a template for cell migration and vascularization. Nevertheless, they fail in many instances due to the pro-inflammatory state of the wound, which includes increased matrix metalloproteases (MMPs) that degrade growth factors. MMPs act on both endogenous and exogenous growth factors that are added to the wound to aid in healing. Therefore, the goal of this project is to improve growth factor treatments delivered in dermal scaffolds by specifically packaging them to survive the diabetic milieu. The growth factor used here, stromal cell-derived factor-1 (SDF-1) is a chemokine that binds to the CXCR4 receptor on stem and progenitor cells; it is down-regulated in diabetes. This dissertation aims to improve SDF-1 action by blocking the pro-inflammatory receptor for advanced glycation end products (RAGE) pathway with soluble RAGE (sRAGE) and improve SDF-1 persistence using a liposome delivery system. To test the effectiveness of SDF-1, a transwell migration assay was developed, where Human Leukemia-60 (HL-60) cells, that contain the CXCR4 receptor, migrate through a porous membrane towards a supply of SDF-1. The major finding using this assay was that, as SDF-1 increases, percent cell migration increases. In addition, when HL-60 cells are pre-treated with 25mM extra glucose in cell media for 24 hours, cell migration decreased compared to cells cultured with plain media and media supplemented with L-glucose. sRAGE treatment reversed this impairment and restored migration. The mechanism causing this phenomenon is the increased superoxide ion (O2-) in increased glucose cultures, which was measured through dihydroethidium. The SDF-1 liposomes were created using standard self assembly methods and also induced HL-60 cell migration in the transwell. SDF-1 liposomes maintained the induction of a calcium response associated with SDF-1 signaling. The SDF-1 liposomes also rapidly and uniformly infiltrated dermal scaffolds Alloderm® and Integra®. In an in vivo diabetic excisional wound model, sRAGE alone was not enough to restore SDF-1 function. However, SDF-1 liposomes sped up wound closure by 1 week over the controls through increased dermal cell proliferation and promotion of wound contraction.

Advisors/Committee Members: Berthiaume, Francois (chair), Sofou, Stavroula (internal member), Moghe, Prabhas (internal member), Hsia, Henry (outside member), Schmidt, Ann Marie (outside member).

Subjects/Keywords: Wounds and injuries – Treatment; Diabetic foot; Diabetes – Complications

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Olekson, Melissa Ann, 1. (2014). Strategies for improving growth factor function in diabetic wounds. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45385/

Chicago Manual of Style (16^th Edition):

Olekson, Melissa Ann, 1986-. “Strategies for improving growth factor function in diabetic wounds.” 2014. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/45385/.

MLA Handbook (7^th Edition):

Olekson, Melissa Ann, 1986-. “Strategies for improving growth factor function in diabetic wounds.” 2014. Web. 15 Apr 2021.

Vancouver:

Olekson, Melissa Ann 1. Strategies for improving growth factor function in diabetic wounds. [Internet] [Doctoral dissertation]. Rutgers University; 2014. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45385/.

Council of Science Editors:

Olekson, Melissa Ann 1. Strategies for improving growth factor function in diabetic wounds. [Doctoral Dissertation]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45385/

12. Heden, Gregory. Carbon nanotube composite scaffolds for differentiation of human neural stem cells.

Degree: MS, Chemical and Biochemical Engineering, 2013, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068878

► Carbon nanotubes have been utilized in a variety of fields due to their unique and extraordinary properties. Here, a process to incorporate single-walled carbon nanotubes… (more)

Subjects/Keywords: Neural stem cells; Nanotubes; Nanomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Heden, G. (2013). Carbon nanotube composite scaffolds for differentiation of human neural stem cells. (Masters Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068878

Chicago Manual of Style (16^th Edition):

Heden, Gregory. “Carbon nanotube composite scaffolds for differentiation of human neural stem cells.” 2013. Masters Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068878.

MLA Handbook (7^th Edition):

Heden, Gregory. “Carbon nanotube composite scaffolds for differentiation of human neural stem cells.” 2013. Web. 15 Apr 2021.

Vancouver:

Heden G. Carbon nanotube composite scaffolds for differentiation of human neural stem cells. [Internet] [Masters thesis]. Rutgers University; 2013. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068878.

Council of Science Editors:

Heden G. Carbon nanotube composite scaffolds for differentiation of human neural stem cells. [Masters Thesis]. Rutgers University; 2013. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000068878

13. Tomasini, Michael D., 1982-. Interfacial interactions of nanoparticles with surfactants and polymers: a computational approach to target biomedical and pharmaceutical systems.

Degree: Biomedical Engineering, 2012, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065282

Subjects/Keywords: Nanoparticles; Nanotechnology; Nanomedicine

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APA (6^th Edition):

Tomasini, Michael D., 1. (2012). Interfacial interactions of nanoparticles with surfactants and polymers: a computational approach to target biomedical and pharmaceutical systems. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065282

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tomasini, Michael D., 1982-. “Interfacial interactions of nanoparticles with surfactants and polymers: a computational approach to target biomedical and pharmaceutical systems.” 2012. Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065282.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tomasini, Michael D., 1982-. “Interfacial interactions of nanoparticles with surfactants and polymers: a computational approach to target biomedical and pharmaceutical systems.” 2012. Web. 15 Apr 2021.

Vancouver:

Tomasini, Michael D. 1. Interfacial interactions of nanoparticles with surfactants and polymers: a computational approach to target biomedical and pharmaceutical systems. [Internet] [Thesis]. Rutgers University; 2012. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065282.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tomasini, Michael D. 1. Interfacial interactions of nanoparticles with surfactants and polymers: a computational approach to target biomedical and pharmaceutical systems. [Thesis]. Rutgers University; 2012. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065282

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Gaudet, Ian Daniel, 1980-. Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels.

Degree: Biomedical Engineering, 2013, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067770

Subjects/Keywords: Colloids in medicine; Collagen

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APA (6^th Edition):

Gaudet, Ian Daniel, 1. (2013). Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067770

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gaudet, Ian Daniel, 1980-. “Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels.” 2013. Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067770.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gaudet, Ian Daniel, 1980-. “Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels.” 2013. Web. 15 Apr 2021.

Vancouver:

Gaudet, Ian Daniel 1. Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels. [Internet] [Thesis]. Rutgers University; 2013. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067770.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gaudet, Ian Daniel 1. Development, characterization, and applications of self-assembling, photocrosslinkable collagen-based hydrogels. [Thesis]. Rutgers University; 2013. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000067770

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Moriarty, Gina M., 1986-. Molecular triggers of disease-related synuclein aggregation in the Parkinson’s environment.

Degree: PhD, Chemistry and Chemical Biology, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55602/

► The works in this thesis explores the challenges involved in recapitulating the environment in which the synuclein family of proteins resides, functions and are active… (more)

▼ The works in this thesis explores the challenges involved in recapitulating the environment in which the synuclein family of proteins resides, functions and are active participants in Parkinson’s disease (PD) pathophysiology. The link between α-synuclein (αS or αsyn) aggregation and PD neurodegeneration has been established by its presence in fibrillar form in intracellular inclusions known as Lewy Bodies (LBs) and associated oligomerization, whereas β-synuclein (βS or βsyn) is thought to be a non-fibrillogenic partner to αS, beneficial to disease. The enclosed work covers two main topics surrounding the environmental sensitivities of these intrinsically disordered proteins’ (IDPs) role in generating disease-associated aggregation: 1) the impact of N-terminal acetylation on αS fibrillation, and 2) the pH responsive source of βS’ inhibited nature. First we generate an N-terminally acetylated αS in response to an investigation of the native oligomeric state of αS in the physiological environment. We find that N-terminal acetylation has minimal impact on the disordered monomeric ensemble of αS and that no evidence of a preference for a tetrameric or other kind of oligomer is found. We find also that acetylated αS forms fibrils of in a similar timeframe and morphology as the non-acetylated form of the protein. While the impact on our view of αS as an intrinsically disordered monomer ensemble is relatively non-consequential, we go on to show that with at least one binding partner, Cu2+, N-terminal acetylation can have a significant impact in vivo. Cu2+ had been shown in vitro to have a unique accelerating effect on αS fibrillation at low, physiological stoichiometries, and given the redox active nature of the metal, and the role of metal dyshomeostasis in neurodegeneration, it had been garnering interest as a significant player in disease-associated αS aggregation. However, we go on to show that N-terminal acetylation occurs at the most significant of three Cu2+ binding sites, blocks binding of the metal and abolishes any fibrillation accelerating effect. Therefore, this work forces reconsideration of how the N-terminally acetylated αS interacts with partners in vivo, and the ultimate role of Cu2+ as an exacerbator of αS pathophysiological aggregation, which may still occur through two other, although much weaker, binding sites at its histidine and C-terminus. In the second part of this thesis, we discover that the model for the physiological environment typically used to draw the conclusion that βS is non-fibrillogenic in contrast to αS, may not be adequate to use to infer things about its intracellular behavior. We discover that mildly acidic pH 5.8, achievable in several intracellular sub-environments, turns on a fibrillation switch and allows βS to form fibrils. We use this pH-responsiveness and a set of α/βS domain-swapped chimeras to study the roots of βS’ less fibril prone nature. We discover through the chimeras that the NAC domain is the most significant determinant of aggregation behavior, and through… Advisors/Committee Members: Baum, Jean (chair), Khare, Sagar (internal member), Case, David (internal member), Moghe, Prabhas (outside member), School of Graduate Studies.

Subjects/Keywords: Parkinson's disease – Pathophysiology; Synucleins

…Chemical Biology, Rutgers University, Piscataway, New Jersey 08854 2 BioMaPS Institute for… …Quantitative Biology, Rutgers University, Piscataway, New Jersey 08854 * Author to whom…

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APA (6^th Edition):

Moriarty, Gina M., 1. (2017). Molecular triggers of disease-related synuclein aggregation in the Parkinson’s environment. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55602/

Chicago Manual of Style (16^th Edition):

Moriarty, Gina M., 1986-. “Molecular triggers of disease-related synuclein aggregation in the Parkinson’s environment.” 2017. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/55602/.

MLA Handbook (7^th Edition):

Moriarty, Gina M., 1986-. “Molecular triggers of disease-related synuclein aggregation in the Parkinson’s environment.” 2017. Web. 15 Apr 2021.

Vancouver:

Moriarty, Gina M. 1. Molecular triggers of disease-related synuclein aggregation in the Parkinson’s environment. [Internet] [Doctoral dissertation]. Rutgers University; 2017. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55602/.

Council of Science Editors:

Moriarty, Gina M. 1. Molecular triggers of disease-related synuclein aggregation in the Parkinson’s environment. [Doctoral Dissertation]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55602/

16. Plourde, Nicole M., 1982-. Structure-binding activity relations of amphiphilic polymers and macrophage scavenger receptors: implications for therapeutic inhibition of atherosclerosis.

Degree: PhD, Chemical and Biochemical Engineering, 2010, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056724

►

Scavenger receptors mediate the uptake of oxidized low density lipoprotein (oxLDL), leading to cholesterol accumulation and the development of atherosclerosis. A promising avenue of interest… (more)

▼

Scavenger receptors mediate the uptake of oxidized low density lipoprotein (oxLDL), leading to cholesterol accumulation and the development of atherosclerosis. A promising avenue of interest in the treatment of cardiovascular disease is the use of a scavenger receptor inhibitor. To this end, a series of polymers were designed based on a mucic acid backbone, aliphatic acid arms and polyethylene glycol tail. Molecular modeling and docking approaches were used to understand the structure-activity relationship (SAR) between the polymers and scavenger receptor class A (SR-A). The polymers containing hydrophobic-bound carboxylate groups were the most favorable binders to the SR-A model as well as the most efficient inhibitors of oxLDL accumulation. Mutant SR-A models were generated by replacing charged residues with alanine. All charged residues in the region were necessary, with Lys60, Lys63 and Lys66 having the greatest effect on binding. However, binding was not mediated by charge alone and the polymer hydrophobic domain adjacent to the carboxylate group was found to be essential. Based on these findings from Chapter 2, the next chapter focused on the polymer backbone. Polymer models were designed to investigate the influence of backbone stereochemistry, cyclic versus linear backbone, and aromatic versus aliphatic backbone. Molecular modeling and docking results indicate the ability of the backbone to favorably position the side chains and “lock” the ligand into position. In vitro results followed those seen in the modeling predictions, with two polymers showing promise. Thus, minute changes in polymer structures can sensitively affect SR-A binding affinities and modulate the competitive inhibition of oxLDL uptake. This thesis also investigated the potential of amphiphilic polymers to target scavenger receptors and deliver a hydrophobic model drug reversing cholesterol accumulation. The polymers encapsulated hydrophobic agonist (GW3965) against nuclear Liver-X receptor α (LXR), which significantly increased the drug uptake over non-polymer delivery. In combination with the encapsulated LXR-agonist, the polymers reduced oxLDL by 88% in vitro. Thus, the thesis findings suggest that the system of amphiphilic polymers exhibited significant tunability for scavenger receptor targeting, which can be exploited both for inhibition of cholesterol uptake as well as modulating cholesterol trafficking.

Advisors/Committee Members: Plourde, Nicole M., 1982- (author), Moghe, Prabhas V (chair), Roth, Charles (internal member), Uhrich, Kathryn E (internal member), Welsh, William J (outside member).

Subjects/Keywords: Atherosclerosis – Treatment; Low density lipoproteins; Cardiovascular receptors; Molecules – Models

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APA (6^th Edition):

Plourde, Nicole M., 1. (2010). Structure-binding activity relations of amphiphilic polymers and macrophage scavenger receptors: implications for therapeutic inhibition of atherosclerosis. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056724

Chicago Manual of Style (16^th Edition):

Plourde, Nicole M., 1982-. “Structure-binding activity relations of amphiphilic polymers and macrophage scavenger receptors: implications for therapeutic inhibition of atherosclerosis.” 2010. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056724.

MLA Handbook (7^th Edition):

Plourde, Nicole M., 1982-. “Structure-binding activity relations of amphiphilic polymers and macrophage scavenger receptors: implications for therapeutic inhibition of atherosclerosis.” 2010. Web. 15 Apr 2021.

Vancouver:

Plourde, Nicole M. 1. Structure-binding activity relations of amphiphilic polymers and macrophage scavenger receptors: implications for therapeutic inhibition of atherosclerosis. [Internet] [Doctoral dissertation]. Rutgers University; 2010. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056724.

Council of Science Editors:

Plourde, Nicole M. 1. Structure-binding activity relations of amphiphilic polymers and macrophage scavenger receptors: implications for therapeutic inhibition of atherosclerosis. [Doctoral Dissertation]. Rutgers University; 2010. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056724

17. Peddada, Lavanya Y., 1983-. Design of poly (alkylene oxide) graft copolymer chemistries for improved antisense drug delivery.

Degree: Biomedical Engineering, 2012, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064160

Subjects/Keywords: Drug delivery systems

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APA (6^th Edition):

Peddada, Lavanya Y., 1. (2012). Design of poly (alkylene oxide) graft copolymer chemistries for improved antisense drug delivery. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064160

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Peddada, Lavanya Y., 1983-. “Design of poly (alkylene oxide) graft copolymer chemistries for improved antisense drug delivery.” 2012. Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064160.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Peddada, Lavanya Y., 1983-. “Design of poly (alkylene oxide) graft copolymer chemistries for improved antisense drug delivery.” 2012. Web. 15 Apr 2021.

Vancouver:

Peddada, Lavanya Y. 1. Design of poly (alkylene oxide) graft copolymer chemistries for improved antisense drug delivery. [Internet] [Thesis]. Rutgers University; 2012. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064160.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Peddada, Lavanya Y. 1. Design of poly (alkylene oxide) graft copolymer chemistries for improved antisense drug delivery. [Thesis]. Rutgers University; 2012. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000064160

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Waite, Carolyn Leigh, 1983-. Engineering tumor-targeted poly(amidoamine) (PAMAM) dendrimers for improved penetration and cellular delivery of short-interfering RNA (siRNA) through solid tumors.

Degree: Chemical and Biochemical Engineering, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063690

Subjects/Keywords: Drug delivery systems; Dendrimers in medicine; Gliomas – Treatment; RNA

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APA (6^th Edition):

Waite, Carolyn Leigh, 1. (2011). Engineering tumor-targeted poly(amidoamine) (PAMAM) dendrimers for improved penetration and cellular delivery of short-interfering RNA (siRNA) through solid tumors. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Waite, Carolyn Leigh, 1983-. “Engineering tumor-targeted poly(amidoamine) (PAMAM) dendrimers for improved penetration and cellular delivery of short-interfering RNA (siRNA) through solid tumors.” 2011. Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Waite, Carolyn Leigh, 1983-. “Engineering tumor-targeted poly(amidoamine) (PAMAM) dendrimers for improved penetration and cellular delivery of short-interfering RNA (siRNA) through solid tumors.” 2011. Web. 15 Apr 2021.

Vancouver:

Waite, Carolyn Leigh 1. Engineering tumor-targeted poly(amidoamine) (PAMAM) dendrimers for improved penetration and cellular delivery of short-interfering RNA (siRNA) through solid tumors. [Internet] [Thesis]. Rutgers University; 2011. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Waite, Carolyn Leigh 1. Engineering tumor-targeted poly(amidoamine) (PAMAM) dendrimers for improved penetration and cellular delivery of short-interfering RNA (siRNA) through solid tumors. [Thesis]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Tresier, Matthew David, 1981-. Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:.

Degree: PhD, Biomedical Engineering, 2009, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914

►

This dissertation advances the field of biomaterials-based tissue engineering via the development of a single cell imaging approach to profile and predict cellular responses. The… (more)

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This dissertation advances the field of biomaterials-based tissue engineering via the development of a single cell imaging approach to profile and predict cellular responses. The methodology at the core of the dissertation characterizes cellular behaviors via the extraction and quantification of cell shape, intensity, textural and spatial distribution features of molecular fluororeporters, referred to as high-content cell imaging. First, we highlight the use of high-content imaging of cell-based fluororeporters to establish and correlate quantifiable metrics of intracellular cytoskeletal features (e.g., descriptors of actin organization) on a set of model biomaterial substrates. The high-content imaging approach is then applied to spatially graded polymer blend substrates of both continuous roughness and discrete chemical compositions in parallel with high-throughput analyses. The imaging approach allowed us to identify the "global" and “high content” structure-property relationships between cell adhesion and biomaterial properties such as polymer chemistry and topography. The approach also identifies features of the actin-based cytoskeleton that respond to minute chemical modifications of the polymer backbone of a combinatorially derived library. In combination with decision tree and artificial neural network analyses, these 24-hour descriptors are used to predict 3-week material-mediated mineralization. The high-content imaging approach is complemented with multi-dimensional scaling (MDS) modeling efforts to project amplified variations in cytoskeletal organization that forecast human mesenchymal stem cell lineage commitment before it is detected via traditional assays. Utilizing early quantitative measures of cytoskeletal morphology (morphometrics) and MDS allows the identification of distinct subpopulations of stem cells that emerge from non-linear combinations of cell shape, texture, density and cytoskeletal organizational features. These clusters allow the prediction of long-term differentiation behaviors of stem cells that manifest days to weeks later than the time of morphometric analysis. The proposed platform represents an ideal approach to probe cell-responses to complex microenvironments as it provides: real time measures of stem cell fates and material induced cell responses, cell-by-cell based analysis that captures the heterogeneity of sample populations, and the ability to parse out lineage commitment in stem cells resulting from multiple stimuli.

Advisors/Committee Members: Tresier, Matthew David, 1981- (author), Moghe, Prabhas (chair), Kohn, Joachim (co-chair), Knight, Doyle (internal member), Androulakis, Ioannis (internal member), Goss Kinzy, Terri (outside member).

Subjects/Keywords: Cell interaction

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APA (6^th Edition):

Tresier, Matthew David, 1. (2009). Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914

Chicago Manual of Style (16^th Edition):

Tresier, Matthew David, 1981-. “Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:.” 2009. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914.

MLA Handbook (7^th Edition):

Tresier, Matthew David, 1981-. “Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:.” 2009. Web. 15 Apr 2021.

Vancouver:

Tresier, Matthew David 1. Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:. [Internet] [Doctoral dissertation]. Rutgers University; 2009. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914.

Council of Science Editors:

Tresier, Matthew David 1. Profiling of cell-substrate interactions using single cell fluororeporter imaging & modeling:. [Doctoral Dissertation]. Rutgers University; 2009. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051914

20. Sparks, Sarah Marie, 1984-. Design, synthesis, and utility of fuctionalized nanoscale amphiphilic macromolecules for biomedical applications.

Degree: PhD, Chemistry and Chemical Biology, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057691

►

Polymeric micelles are spherical assemblies of amphiphilic polymers widely studied for many biomedical applications. Nanoscale amphiphilic macromolecules (AMs) are novel amphiphilic polymers composed of an… (more)

▼

Polymeric micelles are spherical assemblies of amphiphilic polymers widely studied for many biomedical applications. Nanoscale amphiphilic macromolecules (AMs) are novel amphiphilic polymers composed of an alkylated sugar backbone covalently linked to poly(ethylene glycol) (PEG). In aqueous solution, AMs self-assemble to form 10-20 nm micelles with critical micelle concentrations as low as 100 nM, making them more stable than other common micelles. In addition, the basic structure of AMs has multiple points of modification such that the polymer can be modified and evaluated for virtually any application. This work highlights the promise of functionalized AMs as a novel, versatile biomaterial. Carboxy-terminated AMs were previously shown to inhibit highly oxidized low- density lipoprotein (hoxLDL) uptake in macrophage cells. To gain a mechanistic understanding of this inhibition, a series of AMs were designed and synthesized by modifying the basic polymer structure to evaluate several characteristics including: amphiphilicity, PEG chain length, anionic charge location, type of anionic charge, number of anionic charges, rotational motion of the anionic group, and PEG architecture. The optimal AM for inhibiting hoxLDL uptake was determined to be one with a rigid, rotationally-restricted carboxylic acid within the hydrophobic portion of the polymer. Building upon previous work that showed AMs deliver cargo intracellulary, a series of cationic polymers were designed and synthesized for nucleic acid delivery. The cationic moiety was added within the hydrophobic component of the AMs such that when the cationic portion complexed with anionic nucleic acids, the nucleic acids would be localized in the micellar interior. Three cationic AMs were evaluated with varying surface charges. The polymer with the highest surface charge was the most effective at complexing with and delivering small interfering RNA to U87 glioma cells. Finally, without modification, AMs are capable of water-solubilizing hydrophobic drugs. This property can be applied to hydrophobic fluorescent nanocrystals, which are useful for biological imaging. In the last chapter, AMs were utilized to water-solubilize white light-emitting nanocrystals without altering the emission properties of the nanocrystals. By modifying the polymer structure to incorporate functionalities that can coordinate to the surface of the nanocrystals, smaller, water-soluble assemblies that maintain white light-emission were obtained.

Advisors/Committee Members: Sparks, Sarah Marie, 1984- (author), Uhrich, Kathryn E (chair), Seidel, Daniel (internal member), Warmuth, Ralf (internal member), Moghe, Prabhas V (outside member).

Subjects/Keywords: Micelles; Macromolecules; Biomedical materials; Polymers in medicine; Nanotechnology

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APA (6^th Edition):

Sparks, Sarah Marie, 1. (2011). Design, synthesis, and utility of fuctionalized nanoscale amphiphilic macromolecules for biomedical applications. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057691

Chicago Manual of Style (16^th Edition):

Sparks, Sarah Marie, 1984-. “Design, synthesis, and utility of fuctionalized nanoscale amphiphilic macromolecules for biomedical applications.” 2011. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057691.

MLA Handbook (7^th Edition):

Sparks, Sarah Marie, 1984-. “Design, synthesis, and utility of fuctionalized nanoscale amphiphilic macromolecules for biomedical applications.” 2011. Web. 15 Apr 2021.

Vancouver:

Sparks, Sarah Marie 1. Design, synthesis, and utility of fuctionalized nanoscale amphiphilic macromolecules for biomedical applications. [Internet] [Doctoral dissertation]. Rutgers University; 2011. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057691.

Council of Science Editors:

Sparks, Sarah Marie 1. Design, synthesis, and utility of fuctionalized nanoscale amphiphilic macromolecules for biomedical applications. [Doctoral Dissertation]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057691

21. Fantuzzo, Joseph Andrew. Micro-neurocircuitry: modeling brain circuitry with induced neurons for the study of neuropsychiatric disorders.

Degree: PhD, Biomedical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55998/

►

Neuropsychiatric disorders which target defined neural circuits in the brain have been dif-ficult to treat, due to the complexity of the human brain and inaccessibility… (more)

Subjects/Keywords: Microfluidics

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APA (6^th Edition):

Fantuzzo, J. A. (2018). Micro-neurocircuitry: modeling brain circuitry with induced neurons for the study of neuropsychiatric disorders. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55998/

Chicago Manual of Style (16^th Edition):

Fantuzzo, Joseph Andrew. “Micro-neurocircuitry: modeling brain circuitry with induced neurons for the study of neuropsychiatric disorders.” 2018. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/55998/.

MLA Handbook (7^th Edition):

Fantuzzo, Joseph Andrew. “Micro-neurocircuitry: modeling brain circuitry with induced neurons for the study of neuropsychiatric disorders.” 2018. Web. 15 Apr 2021.

Vancouver:

Fantuzzo JA. Micro-neurocircuitry: modeling brain circuitry with induced neurons for the study of neuropsychiatric disorders. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55998/.

Council of Science Editors:

Fantuzzo JA. Micro-neurocircuitry: modeling brain circuitry with induced neurons for the study of neuropsychiatric disorders. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55998/

22. Naczynski, Dominik Jan, 1984-. Developing a multifunctional nanocomposite using albumin-encapsulated rare-earth doped nanoparticles for tumor targeting.

Degree: Chemical and Biochemical Engineering, 2012, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066924

Subjects/Keywords: Nanoparticles; Cancer – Imaging; Albumins

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APA (6^th Edition):

Naczynski, Dominik Jan, 1. (2012). Developing a multifunctional nanocomposite using albumin-encapsulated rare-earth doped nanoparticles for tumor targeting. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Naczynski, Dominik Jan, 1984-. “Developing a multifunctional nanocomposite using albumin-encapsulated rare-earth doped nanoparticles for tumor targeting.” 2012. Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Naczynski, Dominik Jan, 1984-. “Developing a multifunctional nanocomposite using albumin-encapsulated rare-earth doped nanoparticles for tumor targeting.” 2012. Web. 15 Apr 2021.

Vancouver:

Naczynski, Dominik Jan 1. Developing a multifunctional nanocomposite using albumin-encapsulated rare-earth doped nanoparticles for tumor targeting. [Internet] [Thesis]. Rutgers University; 2012. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Naczynski, Dominik Jan 1. Developing a multifunctional nanocomposite using albumin-encapsulated rare-earth doped nanoparticles for tumor targeting. [Thesis]. Rutgers University; 2012. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Chmielowski, Rebecca Ann, 1979-. Design of antioxidant nanotherapeutics for attenuation of atherogenesis and neuroinflammation.

Degree: PhD, Chemical and Biochemical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/55991/

►

Atherosclerosis and Parkinson’s disease are both characterized by the uncontrolled uptake of modified proteins in chronic inflammatory cells, macrophages/microglia. The initial stages of atherosclerosis are… (more)

▼

Atherosclerosis and Parkinson’s disease are both characterized by the uncontrolled uptake of modified proteins in chronic inflammatory cells, macrophages/microglia. The initial stages of atherosclerosis are characterized by oxidation of low density lipoprotein and its uptake by macrophages, which can lead to plaque progression in the arterial wall and possibly heart attack or stroke. Formation and increase of misfolded aggregates of proteins such as alpha synuclein (α-synuclein) are a key factor in several neurodegenerative diseases including Parkinson’s disease. Both classes of diseases are exacerbated in macrophages/microglia due to a strong inflammatory component, which has been challenging to treat. The anti-atheroprotective and anti-inflammatory properties of antioxidants create an attractive target for treatment of atherosclerosis and Parkinson’s disease. We propose a library of antioxidant nanoparticles that have the potential to address critical needs for both atherosclerosis and Parkinson’s disease. For atherosclerosis applications, we advanced the delivery and formulation of antioxidants through the development of ferulic acid-based polymer nanoparticles, which are completely biodegradable and can achieve a sustained and tunable release of ferulic acid to limit macrophage foam cell formation. The ferulic acid-based polymer nanoparticles attenuated macrophage lipogenesis and reactive oxygen species generation. The cellular mechanism of the nanoparticle efficacy involved the down regulation of the expression of three scavenger receptors, which are critical for modulation of lipid uptake in macrophages. For neuroinflammatory applications, we developed a dual antioxidant nanoparticle consisting of ferulic acid and tannic acid, which significantly reduced the generation of -synuclein fibrils. This result suggests that the combination of antioxidants and the configuration of nanoparticle vehicles could be important factors for inhibiting -synuclein fibril formation. Overall, antioxidant nanotherapeutics may be promising technologies for inhibition of early stages of both atherosclerosis and Parkinson’s disease.

Advisors/Committee Members: Moghe, Prabhas V (chair), Roth, Charlie (internal member), Uhrich, Kathryn E (internal member), Baum, Jean (internal member), Joseph, Laurie B (outside member), Tugcu, Nihal (outside member), School of Graduate Studies.

Subjects/Keywords: Atherosclerosis – Treatment

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APA (6^th Edition):

Chmielowski, Rebecca Ann, 1. (2018). Design of antioxidant nanotherapeutics for attenuation of atherogenesis and neuroinflammation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/55991/

Chicago Manual of Style (16^th Edition):

Chmielowski, Rebecca Ann, 1979-. “Design of antioxidant nanotherapeutics for attenuation of atherogenesis and neuroinflammation.” 2018. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/55991/.

MLA Handbook (7^th Edition):

Chmielowski, Rebecca Ann, 1979-. “Design of antioxidant nanotherapeutics for attenuation of atherogenesis and neuroinflammation.” 2018. Web. 15 Apr 2021.

Vancouver:

Chmielowski, Rebecca Ann 1. Design of antioxidant nanotherapeutics for attenuation of atherogenesis and neuroinflammation. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Apr 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55991/.

Council of Science Editors:

Chmielowski, Rebecca Ann 1. Design of antioxidant nanotherapeutics for attenuation of atherogenesis and neuroinflammation. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/55991/

Rutgers University

24. Langowski, Bryan Alfred. Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates.

Degree: Chemistry and Chemical Biology, 2007, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13476

Subjects/Keywords: Tissue engineering; Biomedical engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Langowski, B. A. (2007). Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13476

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Langowski, Bryan Alfred. “Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates.” 2007. Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13476.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Langowski, Bryan Alfred. “Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates.” 2007. Web. 15 Apr 2021.

Vancouver:

Langowski BA. Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates. [Internet] [Thesis]. Rutgers University; 2007. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13476.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Langowski BA. Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates. [Thesis]. Rutgers University; 2007. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13476

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

25. Xu, Jing, 1980-. Albumin-derived nanoparticles: a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells.

Degree: MS, Cell and Developmental Biology, 2010, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053222

► Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types and have been widely investigated as a possible… (more)

Subjects/Keywords: Stem cells; Wound healing; Nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xu, Jing, 1. (2010). Albumin-derived nanoparticles: a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells. (Masters Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053222

Chicago Manual of Style (16^th Edition):

Xu, Jing, 1980-. “Albumin-derived nanoparticles: a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells.” 2010. Masters Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053222.

MLA Handbook (7^th Edition):

Xu, Jing, 1980-. “Albumin-derived nanoparticles: a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells.” 2010. Web. 15 Apr 2021.

Vancouver:

Xu, Jing 1. Albumin-derived nanoparticles: a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells. [Internet] [Masters thesis]. Rutgers University; 2010. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053222.

Council of Science Editors:

Xu, Jing 1. Albumin-derived nanoparticles: a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells. [Masters Thesis]. Rutgers University; 2010. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053222

Rutgers University

26. Paladini, Bryan. Gold nanoparticle dimers for SERS-based targeted detection of human glioblastoma cells.

Degree: MS, Materials Science and Engineering, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063561

► Gold nanospheres are linked by a Raman active dithiolated linker molecule forming dimer and trimer assemblies. These nanoparticles are capped with polyethylene glycol for stability… (more)

Subjects/Keywords: Nanoparticles; Nervous system—Tumors – Treatment

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paladini, B. (2011). Gold nanoparticle dimers for SERS-based targeted detection of human glioblastoma cells. (Masters Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063561

Chicago Manual of Style (16^th Edition):

Paladini, Bryan. “Gold nanoparticle dimers for SERS-based targeted detection of human glioblastoma cells.” 2011. Masters Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063561.

MLA Handbook (7^th Edition):

Paladini, Bryan. “Gold nanoparticle dimers for SERS-based targeted detection of human glioblastoma cells.” 2011. Web. 15 Apr 2021.

Vancouver:

Paladini B. Gold nanoparticle dimers for SERS-based targeted detection of human glioblastoma cells. [Internet] [Masters thesis]. Rutgers University; 2011. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063561.

Council of Science Editors:

Paladini B. Gold nanoparticle dimers for SERS-based targeted detection of human glioblastoma cells. [Masters Thesis]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063561

Rutgers University

27. Looi, Jennifer C. Noninvasive assessment of mental, physical and respiratory stressors on cardiovascular function.

Degree: MS, Biomedical Engineering, 2007, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15761

► Stress is encountered in daily life from relationships with people, work obligations, and our environment. Often viewed from the realm of the social sciences, this… (more)

▼ Stress is encountered in daily life from relationships with people, work obligations, and our environment. Often viewed from the realm of the social sciences, this field of study was perceived to be limited to descriptive measures, which only captured information discretely. As medicine started to investigate the biological ramifications of the stress response, it elevated the importance of stress as a precursor to several chronic diseases, which manifest both physically and mentally. As a result, many sub-areas of research have emerged to examine the relationship of stress to various systems within the human body, which include the endocrine, cardiovascular, respiratory systems. Growing understanding of the biological realities of stress will help disentangle the multiple connotations of stress. It can aid in producing better targeted medications and treatments. This thesis compared three types of stressors and sought to determine which cardiovascular parameters were most sensitive to stress. Young volunteers were subjected to mental, physical and respiratory stressors while their variability of heart rate, blood pressure and blood flow velocity were measured to examine the role of stress on the cardiovascular system. From comparisons between the rest and different stressor interventions for each subject, statistics from paired T tests show significant differences exist between each stressor and rest phase. In addition, testing of the null hypothesis against T wave amplitude, QT interval, R-P onset, frequency, beats per minute, change in blood pressure and change in blood flow velocity reveal the most sensitive cardiovascular parameters are frequency, beats per minute, blood pressure and flow velocity. These cardiovascular parameters can be used as indicators to study the course of coronary artery disease, myocardial infarction, atherosclerosis and hypertension. The present study can be improved by including measurement of glucose and stress hormones during stressor interventions to enhance our understanding of the stress response. This would aid in studying other stress-induced diseases outside the realm of the cardiovascular system. Advisors/Committee Members: Looi, Jennifer C. (author), Moghe, Prabhas (chair), Li, John (internal member), Shoane, George (internal member), Drzewiecki, Gary (internal member).

Subjects/Keywords: Stress (Physiology); Heart – Effect of stress on

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Looi, J. C. (2007). Noninvasive assessment of mental, physical and respiratory stressors on cardiovascular function. (Masters Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15761

Chicago Manual of Style (16^th Edition):

Looi, Jennifer C. “Noninvasive assessment of mental, physical and respiratory stressors on cardiovascular function.” 2007. Masters Thesis, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15761.

MLA Handbook (7^th Edition):

Looi, Jennifer C. “Noninvasive assessment of mental, physical and respiratory stressors on cardiovascular function.” 2007. Web. 15 Apr 2021.

Vancouver:

Looi JC. Noninvasive assessment of mental, physical and respiratory stressors on cardiovascular function. [Internet] [Masters thesis]. Rutgers University; 2007. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15761.

Council of Science Editors:

Looi JC. Noninvasive assessment of mental, physical and respiratory stressors on cardiovascular function. [Masters Thesis]. Rutgers University; 2007. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15761

Rutgers University

28. Wang, Jinzhong, 1979-. Synthesis and evaluation of novel amphiphilic macromolecules as drug carriers and therapeutics.

Degree: PhD, Chemistry and Chemical Biology, 2007, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16792

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Novel amphiphilic star-like macromolecules (ASM) and amphiphilic scorpion-like macromolecules (AScM) with double-chained and single-chained tails were synthesized and characterized. All macromolecules are composed of mucic… (more)

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Novel amphiphilic star-like macromolecules (ASM) and amphiphilic scorpion-like macromolecules (AScM) with double-chained and single-chained tails were synthesized and characterized. All macromolecules are composed of mucic acid-based hydrophobic &amp;amp;amp;#08220;heads&amp;amp;amp;#08221; and poly(ethylene glycol)-based hydrophilic &amp;amp;amp;#08220;tails&amp;amp;amp;#08221;. Two different ASMs (M12P5 and M12P2x2) and two different AScMs (NC12P5 and NC12P2x2) were investigated to explore how branched PEG chains influence particle size, water-solubility, drug loading capacity, drug release rate and micelle stability. A hydrophobic, anti-inflammatory drug (indomethacin) was used to evaluate the encapsulation ability and release rate from the macromolecules. The double-chained macromolecules reduced the micellar sizes (10 nm for AScM, 22 nm for ASM) compared to single-chained macromolecules (18 nm for AScM, 48 nm for ASM). Through oil/water emulsion methods, drug-loading efficiency of ASM reached nearly 50%, higher than the self-assembled micelle AScMs, which display a drug-loading efficiency 30%. Indomethacin- loaded ASM released 52% of free drug within 50 hours, compared with 78% for AScM. Dynamic light scattering experiments showed that ASM minimized protein interactions. Double-chained macromolecules perform as well or better than single-chained ones as drug delivery systems. Several AScMs, which bear carboxylate groups on hydrophilic and hydrophobic domains, were also prepared. These macromolecules formed extremely stable micelles in aqueous solution with an average size of 20-35 nm and critical micelle concentration (CMC) as low as 10-7 M. Zeta potential values and micellar sizes in neutral buffer solutions correlated well with the carboxylate location and numbers. All macromolecules are capable of inhibiting unregulated uptake of highly-oxidized low density lipoproteins (LDL) by macrophages. This inhibition is caused by the interaction of scavenger receptors with negatively charged macromolecules, and closely responds to the number and location of negative charges. The AScM with one carboxylate at the hydrophobic domain and one carboxylate at hydrophilic domain exhibited the best LDL inhibition. To further enhance the treatment, a ligand GW 3965 was loaded into the AScM micelle.

Advisors/Committee Members: Wang, Jinzhong, 1979- (author), Uhrich, Kathryn (chair), Castner, Edward (internal member), Warmuth, Ralf (internal member), Moghe, Prabhas (outside member).

Subjects/Keywords: Drug delivery systems; Drug carriers (Pharmacy); Pharmaceutical technology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Jinzhong, 1. (2007). Synthesis and evaluation of novel amphiphilic macromolecules as drug carriers and therapeutics. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16792

Chicago Manual of Style (16^th Edition):

Wang, Jinzhong, 1979-. “Synthesis and evaluation of novel amphiphilic macromolecules as drug carriers and therapeutics.” 2007. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16792.

MLA Handbook (7^th Edition):

Wang, Jinzhong, 1979-. “Synthesis and evaluation of novel amphiphilic macromolecules as drug carriers and therapeutics.” 2007. Web. 15 Apr 2021.

Vancouver:

Wang, Jinzhong 1. Synthesis and evaluation of novel amphiphilic macromolecules as drug carriers and therapeutics. [Internet] [Doctoral dissertation]. Rutgers University; 2007. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16792.

Council of Science Editors:

Wang, Jinzhong 1. Synthesis and evaluation of novel amphiphilic macromolecules as drug carriers and therapeutics. [Doctoral Dissertation]. Rutgers University; 2007. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16792

Rutgers University

29. Haders, Daniel Joseph. Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite.

Degree: PhD, Biomedical Engineering, 2008, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17321

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Hydroxyapatite (HA), Ca10(PO4)6(OH)2, the stoichiometric equivalent of the ceramic phase of bone, is the preferred material for hard tissue replacement due to its bioactivity. However,… (more)

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Hydroxyapatite (HA), Ca10(PO4)6(OH)2, the stoichiometric equivalent of the ceramic phase of bone, is the preferred material for hard tissue replacement due to its bioactivity. However, bioinert metals are utilized in load-bearing orthopedic applications due to the poor mechanical properties of HA. Consequently, attention has been given to HA coatings for metallic orthopedic implants to take advantage of the bioactivity of HA and the mechanical properties of metals. Commercially, the plasma spray process (PS-HA) is the method most often used to deposit HA films on metallic implants. Since its introduction in the 1980's, however, concerns have been raised about the consequences of PS-HA's low crystallinity, lack of phase purity, lack of film-substrate chemical adhesion, passivation properties, and difficulty in coating complex geometries. Thus, there is a need to develop inexpensive reproducible next-generation HA film deposition techniques, which deposit high crystallinity, phase pure, adhesive, passivating, conformal HA films on clinical metallic substrates. The aim of this dissertation was to intelligently synthesize and characterize the material and biological properties of HA films on metallic substrates synthesized by hydrothermal crystallization, using thermodynamic phase diagrams as the starting point. In three overlapping interdisciplinary studies the potential of using ethylenediamine-tetraacetic acid/triethyl phosphate (EDTA/TEP) doubly regulated hydrothermal crystallization to deposit HA films, the TEP-regulated, time-and-temperature-dependent process by which films were deposited, and the bioactivity of crystallographically engineered films were investigated. Films were crystallized in a 0.232 molal Ca(NO3)2-0.232 molal EDTA-0.187 molal TEP-1.852 molal KOH-H2O chemical system at 200° C. Thermodynamic phase diagrams demonstrated that the chosen conditions were expected to produce Ca-P phase pure HA, which was experimentally confirmed. EDTA regulation of Ca2+ concentration enabled the HA crystallization process to be growth dominated, producing films composed of high crystallinity, hexagonal grains on multiple metallic substrates. TEP regulation of HA crystallization enabled the deposition of an adhesive CaTiO3 intermediate layer, and then HA in a continuous, phase sequenced process on Ti6Al4V substrates, the first such process reported in the hydrothermal HA literature. The HA film was found to be deposited by a passivating competitive growth mechanism that enabled the [0001] crystallographic orientation of hexagonal single crystals to be engineered with synthesis time. Bioactivity analysis demonstrated that films were bioactive and bone bonding. Together, these results suggest that these HA films are candidates for use on metallic orthopedic implants, namely Ti6Al4V.

Advisors/Committee Members: Haders, Daniel Joseph (author), Riman, Richard (chair), Denhardt, David (internal member), Moghe, Prabhas (internal member), Mann, Adrian (internal member).

Subjects/Keywords: Bioactive compounds – Therapeutic use; Hydroxyapatite – Biocompatibility; Orthopedic implants – Materials – Biocompatibility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haders, D. J. (2008). Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17321

Chicago Manual of Style (16^th Edition):

Haders, Daniel Joseph. “Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite.” 2008. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17321.

MLA Handbook (7^th Edition):

Haders, Daniel Joseph. “Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite.” 2008. Web. 15 Apr 2021.

Vancouver:

Haders DJ. Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite. [Internet] [Doctoral dissertation]. Rutgers University; 2008. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17321.

Council of Science Editors:

Haders DJ. Synthesis and characterization of TEP-EDTA-regulated bioactive hydroxyapatite. [Doctoral Dissertation]. Rutgers University; 2008. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17321

Rutgers University

30. Moore, Rebecca. Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors.

Degree: PhD, Biomedical Engineering, 2008, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535

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The field of stem cell bioengineering can potentially revolutionize cell-based therapies for functional replacement of complex systems like the liver and nervous system. Despite significant… (more)

Subjects/Keywords: Cell differentiation; Cell adhesion molecules; Cells – Growth

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moore, R. (2008). Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535

Chicago Manual of Style (16^th Edition):

Moore, Rebecca. “Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors.” 2008. Doctoral Dissertation, Rutgers University. Accessed April 15, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535.

MLA Handbook (7^th Edition):

Moore, Rebecca. “Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors.” 2008. Web. 15 Apr 2021.

Vancouver:

Moore R. Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors. [Internet] [Doctoral dissertation]. Rutgers University; 2008. [cited 2021 Apr 15]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535.

Council of Science Editors:

Moore R. Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors. [Doctoral Dissertation]. Rutgers University; 2008. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535

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