+publisher:"Rutgers University" +contributor:("Grumet, Martin")

Rutgers University

1. Kabat, Maciej, 1995-. Alginate encapsulation of cells to provide a commercially viable delivery for cell therapy.

Degree: MS, Biomedical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/57625/

► A leading cause of disease and death for all age groups are syndromes characterized by widespread uncontrollable inflammation. Severe trauma induces pro-inflammatory responses, increasing the… (more)

▼ A leading cause of disease and death for all age groups are syndromes characterized by widespread uncontrollable inflammation. Severe trauma induces pro-inflammatory responses, increasing the risk of complications. Mesenchymal Stem Cells (MSCs) have the remarkable ability of altering the immune response by emitting soluble anti-inflammatory factors, which rebalance the immune system and thereby may prevent additional damage and promote recovery. Furthermore, alginate encapsulation of MSCs has proven to be an effective long-term delivery method. Encapsulation of MSCs involves a mixture of alginate and cells, which are cross-linked into microspheres with divalent cations. We hypothesized that optimization and scale-up of encapsulation of cells in alginate microspheres will provide a commercially viable solution for delivery of cell therapy. To analyze trends and characteristics of MSC clinical trials, a novel database was constructed using data derived from Clinical.Trial.gov, including data on disease targets, sources of cells, doses being delivered and routes of administration. Among the hundreds of clinical trials using free MSCs that were analyzed, several critical uncontrollable parameters may contribute to the relatively poor success rates. This includes rapid clearance in various organs, and uncertainty of numbers of surviving cells over time in target locations after transplantation. Encapsulation of MSCs in may overcome many of these challenges by prolonging MSC survival and localizing the cells in desired sites. Alginate encapsulation involves extrusion of a mixture of cells suspended in alginate into microdroplets that crosslinked into microspheres after falling into a bath with divalent cations. We compared two modes of encapsulation, fluidic control and pressure control, holding these parameters fixed. Pressure control is advantageous over fluidic control by reducing transient and residual flow, and eliminating dead volume. Our results with pressure control show that as inner needle diameter increases flow rates increase by a power of 4. Shortening the needle increases the flow rate linearly. Thus shorter and wider needles have higher flow rates, which yield larger capsules and increased yields of encapsulated cells after a given run time using the pressure control mode. The wide dynamic range with pressure control is advantageous over the more limited fluidic control by varying needle diameters. Measuring the number of cells per capsule is technically challenging and often not reported. The most common method for quantifying the number of live/dead cells per alginate microcapsule is imaging stained encapsulated cells using a confocal microscope. While confocal microscopy is advantageous in imaging all planes throughout a capsule, they have some disadvantages including high cost of imaging, long image acquisition time and technical limitation of measuring the number of cells per capsule in large and high density capsules. To overcome these problems, we have developed an alginate depolymerization method to… Advisors/Committee Members: Grumet, Martin (chair), School of Graduate Studies.

Subjects/Keywords: Drug delivery systems; Cellular therapy

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APA (6^th Edition):

Kabat, Maciej, 1. (2018). Alginate encapsulation of cells to provide a commercially viable delivery for cell therapy. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/57625/

Chicago Manual of Style (16^th Edition):

Kabat, Maciej, 1995-. “Alginate encapsulation of cells to provide a commercially viable delivery for cell therapy.” 2018. Masters Thesis, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/57625/.

MLA Handbook (7^th Edition):

Kabat, Maciej, 1995-. “Alginate encapsulation of cells to provide a commercially viable delivery for cell therapy.” 2018. Web. 05 Mar 2021.

Vancouver:

Kabat, Maciej 1. Alginate encapsulation of cells to provide a commercially viable delivery for cell therapy. [Internet] [Masters thesis]. Rutgers University; 2018. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57625/.

Council of Science Editors:

Kabat, Maciej 1. Alginate encapsulation of cells to provide a commercially viable delivery for cell therapy. [Masters Thesis]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57625/

Rutgers University

2. Bennett, Neal, 1989-. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/

►

Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by motor dysfunction, eventual cognitive impairment and dementia in advanced stages. These symptoms arise as a… (more)

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Parkinson's disease (PD) is a progressive neurodegenerative disorder marked by motor dysfunction, eventual cognitive impairment and dementia in advanced stages. These symptoms arise as a result of decreased activity or death of dopaminergic (DA) neurons in the substantia nigra region of the brain, leading to dopamine depletion in the striatum disruption of neuronal circuitry in the basal ganglia. The current most common treatment for PD is levodopa, which can be converted into dopamine by surviving DA neurons, offering temporary relief of the motor dysfunction symptoms of PD. However, a key disadvantage of levodopa as a therapeutic strategy is that it does little to address the progression of PD and symptoms typically worsen as DA neurons continue to degenerate or die. Based on the a critical need for more comprehensive therapeutic approaches to PD that do more than relieve dopamine deficiency, but also disrupt the factors causing disease progression, the work in this dissertation focus on our efforts to address key aspects of Parkinson's disease pathology: neuronal degeneration, neuroinflammation, and synucleinopathy. To address neuronal degeneration, we investigated the potential for 3D fibrous synthetic substrates to support and transplant populations of human reprogrammed neurons into the brain. We found that fibrous substrate geometries could be tuned to shift reprogrammed cell populations towards either neuronal differentiation or maintenance of pluripotency. Microscale scaffolds generated from these fibrous substrates improved transplanted neuronal survival by at least an order of magnitude over traditional cell transplantation techniques. These proof-of-concept studies could be used to inform the future design of transplantable scaffolds supporting neurons reprogrammed to best address DA deficiency in PD. To address neuroinflammation and synucleinopathy, we examined the potential of microglia-targeting nanotherapeutics. We first identified scavenger receptors as a microglial receptor for α-synuclein (ASYN), a protein that forms characteristic protein aggregates and activates microglia in PD. We then designed nanoparticles targeting this interaction using synthetic amphiphilic scavenger receptor ligands. These amphiphilic molecules could reduce ASYN internalization and intracellular aggregation by microglia. We used nanoparticle constructs made using these synthetic ligands to target delivery of antioxidants to microglia, decreasing microglial activation in response to aggregated ASYN in vitro and in vivo. In summary, the studies described in this dissertation establish a valuable foundation for future therapeutic strategies addressing key features of PD pathophysiology and progression.

Advisors/Committee Members: Moghe, Prabhas V (chair), Pang, Zhiping (internal member), Grumet, Martin (internal member), Baum, Jean (outside member).

Subjects/Keywords: Parkinson's disease – Treatment; Nanobiotechnology

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APA (6^th Edition):

Bennett, Neal, 1. (2016). Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51233/

Chicago Manual of Style (16^th Edition):

Bennett, Neal, 1989-. “Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.” 2016. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51233/.

MLA Handbook (7^th Edition):

Bennett, Neal, 1989-. “Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease.” 2016. Web. 05 Mar 2021.

Vancouver:

Bennett, Neal 1. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/.

Council of Science Editors:

Bennett, Neal 1. Design of reprogrammed neuronal transplantation and nanobiomaterial-based microglial therapeutic technologies for management of Parkinson's disease. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51233/

Rutgers University

3. Hsu, Shao-Yun, 1983-. The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model.

Degree: PhD, Neuroscience, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/50009/

►

The Multicenter Animal Spinal Cord Injury Study (MASCIS) standardized spinal cord injury (SCI) in Long-Evans hooded and Sprague-Dawley rats. In this thesis, we extended the… (more)

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The Multicenter Animal Spinal Cord Injury Study (MASCIS) standardized spinal cord injury (SCI) in Long-Evans hooded and Sprague-Dawley rats. In this thesis, we extended the MASCIS model to Fischer 344 (F344) rats for the following reasons. First, F344 rats are immune-compatible with each other. Cells can be transplanted from one F344 rat to another without immunosuppression. Second, our laboratory developed a strain of F344 rats that express green fluorescent protein (GFP). When transplanted into regular F344 rats, GFP F344 rat cells can be readily identified. Third, F344 rats are isogenic. They should have more uniform and replicable responses to injury and therapies. Finally, this F344 model allows us to assess effects of allogeneic cell transplants with and without immunosuppressive drugs, such as cyclosporin-A (CsA), as well as the effects of immunosuppressive drugs on transplanted cells. We chose to assess olfactory ensheathing glial (OEG) cell transplants because many investigators have reported that these cells improve motor recovery in rat SCI models. Our results show that F344 rats can be reproducibly injured with the MASCIS model and that OEG transplants significantly improve locomotor recovery when transplanted without CsA. However, we encountered several unexpected findings. Female rats recovered more than male rats even though the animals were age-matched, studies of the spinal cords revealed no difference in size and anatomy of age-matched male and female rats, and 24-hour lesion volumes in male and female spinal cords were the same. OEG transplants improved recovery of male rats, almost as well as untreated female rats. CsA therapy improved recovery in both male and female rats. We conclude that the MASCIS model causes reproducible injury to spinal cords of F344 rats, age-matched female rats recover better locomotor function than male rats, OEG cells can be transplanted from GFP F344 rats to wild-type F344 rats without immune rejection, OEG transplants improve functional recovery particularly in male rats, CsA improved locomotor recovery in both male and female F344 rats. The MASCIS impactor model should be very useful for assessing mechanisms underlying gender differences, OEG and CsA effects on transplanted cells and recovery mechanisms.

Advisors/Committee Members: Young, Wise (chair), Grumet, Martin (co-chair), Hsu, Shu-Chan (co-chair), Bresnahan, Jacqueline (outside member).

Subjects/Keywords: Cyclosporine; Spinal cord – Wounds and injuries

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APA (6^th Edition):

Hsu, Shao-Yun, 1. (2016). The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/50009/

Chicago Manual of Style (16^th Edition):

Hsu, Shao-Yun, 1983-. “The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model.” 2016. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/50009/.

MLA Handbook (7^th Edition):

Hsu, Shao-Yun, 1983-. “The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model.” 2016. Web. 05 Mar 2021.

Vancouver:

Hsu, Shao-Yun 1. The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/50009/.

Council of Science Editors:

Hsu, Shao-Yun 1. The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/50009/

Rutgers University

4. Li, Ying, 1987-. Gene regulation during central nervous system development and post-injury regeneration.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49255/

►

Central nervous system (CNS) development and post-injury neurogenesis require accurate coordination of neural stem cell proliferation, progenitor cell differentiation, neuron, glia migration and maturation, and… (more)

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Central nervous system (CNS) development and post-injury neurogenesis require accurate coordination of neural stem cell proliferation, progenitor cell differentiation, neuron, glia migration and maturation, and synapse formation between axons and dendrites. Such systems with high complexity require strict temporal and spatial control via several levels of regulation, in which the transcription regulation is one of the most critical steps. The developmental and injury-repair process involves over 18,000 genes, for majority of which the molecular mechanism governing their transcription remains largely unknown. In an attempt to address this question, four projects were conducted focusing on two levels of transcription regulation: i.e., chromatin modification, and the interaction of cis-acting regulatory sequences with trans-acting protein factors. Computational methods were adopted to analyze the sequences of the cis-elements and iii make predictions for their interacting transcription factors (TFs). The functional roles of these cis- and trans-elements were further determined in vivo and in vitro. The following findings are presented: 1) the function of DNA topoisomerase II beta (Top2b) in proper laminar formation and cell survival during retinal development; 2) the development of computational method for identifying gene regulatory networks involving enhancers and master TFs that are important in retinal cell differentiation; 3) the mechanism of Notch1 regulation in neural stem/progenitor cells via the interaction between Nkx6.1 and a CNS specific enhancer CR2 during the development of the spinal cord interneurons; and 4) the role of CR2 in aNSC activation after injury. Findings from this dissertation provide new insights into the molecular mechanisms underlying transcription regulation during CNS development and post-injury neurogenesis. They can also serve as a basis for future development of gene therapies and regenerative medicine for neurological disorders including spinal cord injury.

Advisors/Committee Members: Cai, Li (chair), Firestein, Bonnie (internal member), Grumet, Martin (internal member), Kwan, Kelvin (outside member), Rasin, Mladen-Roko (outside member).

Subjects/Keywords: Genetic regulation; Developmental neurobiology

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APA (6^th Edition):

Li, Ying, 1. (2016). Gene regulation during central nervous system development and post-injury regeneration. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49255/

Chicago Manual of Style (16^th Edition):

Li, Ying, 1987-. “Gene regulation during central nervous system development and post-injury regeneration.” 2016. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49255/.

MLA Handbook (7^th Edition):

Li, Ying, 1987-. “Gene regulation during central nervous system development and post-injury regeneration.” 2016. Web. 05 Mar 2021.

Vancouver:

Li, Ying 1. Gene regulation during central nervous system development and post-injury regeneration. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49255/.

Council of Science Editors:

Li, Ying 1. Gene regulation during central nervous system development and post-injury regeneration. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49255/

5. Tzatzalos, Evangeline, 1983-. A regulatory element for interneuron progenitors in the developing vertebrate central nervous system.

Degree: Biomedical Engineering, 2012, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065283

Subjects/Keywords: Nerve tissue proteins; Nerve growth factor; Nervous system – Growth

…Neuroscience at Rutgers University. Heterozygous mutants reeler+/- were crossed with Notch1CR2-βGP…

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APA (6^th Edition):

Tzatzalos, Evangeline, 1. (2012). A regulatory element for interneuron progenitors in the developing vertebrate central nervous system. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065283

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tzatzalos, Evangeline, 1983-. “A regulatory element for interneuron progenitors in the developing vertebrate central nervous system.” 2012. Thesis, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065283.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tzatzalos, Evangeline, 1983-. “A regulatory element for interneuron progenitors in the developing vertebrate central nervous system.” 2012. Web. 05 Mar 2021.

Vancouver:

Tzatzalos, Evangeline 1. A regulatory element for interneuron progenitors in the developing vertebrate central nervous system. [Internet] [Thesis]. Rutgers University; 2012. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065283.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tzatzalos, Evangeline 1. A regulatory element for interneuron progenitors in the developing vertebrate central nervous system. [Thesis]. Rutgers University; 2012. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000065283

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Barminko, Jeffrey Avi, 1985-. Encapsulated Mesenchymal stromal cells for spinal cord injury repair.

Degree: Biomedical Engineering, 2012, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066610

Subjects/Keywords: Spinal cord – Wounds and injuries – Treatment; Mesenchymal stem cells – Transplantation

10 more images…

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APA (6^th Edition):

Barminko, Jeffrey Avi, 1. (2012). Encapsulated Mesenchymal stromal cells for spinal cord injury repair. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066610

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Barminko, Jeffrey Avi, 1985-. “Encapsulated Mesenchymal stromal cells for spinal cord injury repair.” 2012. Thesis, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066610.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Barminko, Jeffrey Avi, 1985-. “Encapsulated Mesenchymal stromal cells for spinal cord injury repair.” 2012. Web. 05 Mar 2021.

Vancouver:

Barminko, Jeffrey Avi 1. Encapsulated Mesenchymal stromal cells for spinal cord injury repair. [Internet] [Thesis]. Rutgers University; 2012. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066610.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barminko, Jeffrey Avi 1. Encapsulated Mesenchymal stromal cells for spinal cord injury repair. [Thesis]. Rutgers University; 2012. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000066610

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

7. Davila, Jorge, 1977-. MIR-9 targets OC2 in proliferating and differentiating neural stem cells.

Degree: PhD, Cell and Developmental Biology, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061184

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MicroRNAs are key regulators of biological processes. In this thesis we identify mir-9 as a critical regulator during NSC proliferation and neuronal differentiation. Interestingly the… (more)

Subjects/Keywords: RNA; Neural stem cells

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APA (6^th Edition):

Davila, Jorge, 1. (2011). MIR-9 targets OC2 in proliferating and differentiating neural stem cells. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061184

Chicago Manual of Style (16^th Edition):

Davila, Jorge, 1977-. “MIR-9 targets OC2 in proliferating and differentiating neural stem cells.” 2011. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061184.

MLA Handbook (7^th Edition):

Davila, Jorge, 1977-. “MIR-9 targets OC2 in proliferating and differentiating neural stem cells.” 2011. Web. 05 Mar 2021.

Vancouver:

Davila, Jorge 1. MIR-9 targets OC2 in proliferating and differentiating neural stem cells. [Internet] [Doctoral dissertation]. Rutgers University; 2011. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061184.

Council of Science Editors:

Davila, Jorge 1. MIR-9 targets OC2 in proliferating and differentiating neural stem cells. [Doctoral Dissertation]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000061184

Rutgers University

8. Ricupero, Christopher L. Epigenetic regulation and transcription factor programming enhances neurogenesis in neural stem cells.

Degree: Neuroscience, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063582

Subjects/Keywords: Developmental neurobiology; Epigenesis; Neural stem cells

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APA (6^th Edition):

Ricupero, C. L. (2011). Epigenetic regulation and transcription factor programming enhances neurogenesis in neural stem cells. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ricupero, Christopher L. “Epigenetic regulation and transcription factor programming enhances neurogenesis in neural stem cells.” 2011. Thesis, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ricupero, Christopher L. “Epigenetic regulation and transcription factor programming enhances neurogenesis in neural stem cells.” 2011. Web. 05 Mar 2021.

Vancouver:

Ricupero CL. Epigenetic regulation and transcription factor programming enhances neurogenesis in neural stem cells. [Internet] [Thesis]. Rutgers University; 2011. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ricupero CL. Epigenetic regulation and transcription factor programming enhances neurogenesis in neural stem cells. [Thesis]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

9. Carlson, Aaron L., 1985-. Engineered synthetic microenvironments for human pluripotent stem cells: applications for neuronal regeneration.

Degree: Biomedical Engineering, 2013, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/41738/

Subjects/Keywords: Stem cells – Research; Regenerative medicine; Nervous system – Regeneration

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APA (6^th Edition):

Carlson, Aaron L., 1. (2013). Engineered synthetic microenvironments for human pluripotent stem cells: applications for neuronal regeneration. (Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/41738/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carlson, Aaron L., 1985-. “Engineered synthetic microenvironments for human pluripotent stem cells: applications for neuronal regeneration.” 2013. Thesis, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/41738/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carlson, Aaron L., 1985-. “Engineered synthetic microenvironments for human pluripotent stem cells: applications for neuronal regeneration.” 2013. Web. 05 Mar 2021.

Vancouver:

Carlson, Aaron L. 1. Engineered synthetic microenvironments for human pluripotent stem cells: applications for neuronal regeneration. [Internet] [Thesis]. Rutgers University; 2013. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41738/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carlson, Aaron L. 1. Engineered synthetic microenvironments for human pluripotent stem cells: applications for neuronal regeneration. [Thesis]. Rutgers University; 2013. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41738/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

10. Cherry, Jocie Fatima, 1978-. Engineered presentation of neural cell adhesion molecules for directed neural and neural stem cell behaviors.

Degree: Biomedical Engineering, 2013, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/41742/

Subjects/Keywords: Cytology – Research; Nervous system – Wounds and injuries; Regenerative medicine; Stem cells – Transplantation

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APA (6^th Edition):

Cherry, Jocie Fatima, 1. (2013). Engineered presentation of neural cell adhesion molecules for directed neural and neural stem cell behaviors. (Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/41742/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cherry, Jocie Fatima, 1978-. “Engineered presentation of neural cell adhesion molecules for directed neural and neural stem cell behaviors.” 2013. Thesis, Rutgers University. Accessed March 05, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/41742/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cherry, Jocie Fatima, 1978-. “Engineered presentation of neural cell adhesion molecules for directed neural and neural stem cell behaviors.” 2013. Web. 05 Mar 2021.

Vancouver:

Cherry, Jocie Fatima 1. Engineered presentation of neural cell adhesion molecules for directed neural and neural stem cell behaviors. [Internet] [Thesis]. Rutgers University; 2013. [cited 2021 Mar 05]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41742/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cherry, Jocie Fatima 1. Engineered presentation of neural cell adhesion molecules for directed neural and neural stem cell behaviors. [Thesis]. Rutgers University; 2013. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/41742/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

11. Moore, Rebecca. Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors.

Degree: PhD, Biomedical Engineering, 2008, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535

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The field of stem cell bioengineering can potentially revolutionize cell-based therapies for functional replacement of complex systems like the liver and nervous system. Despite significant… (more)

Subjects/Keywords: Cell differentiation; Cell adhesion molecules; Cells – Growth

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APA (6^th Edition):

Moore, R. (2008). Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535

Chicago Manual of Style (16^th Edition):

Moore, Rebecca. “Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors.” 2008. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535.

MLA Handbook (7^th Edition):

Moore, Rebecca. “Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors.” 2008. Web. 05 Mar 2021.

Vancouver:

Moore R. Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors. [Internet] [Doctoral dissertation]. Rutgers University; 2008. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535.

Council of Science Editors:

Moore R. Differentiation of human and murine embryonic stem cells: studies on the combined roles of adhesion molecules and growth factors. [Doctoral Dissertation]. Rutgers University; 2008. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17535

Rutgers University

12. Florek, Charles A., 1980-. A synthetic biomaterials approach to the prevention of postsurgical adhesions in neurosurgery.

Degree: PhD, Biomedical Engineering, 2010, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056345

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In the treatment of spinal stenosis and disc herniation, the lamina and ligamentum flavum are resected to access the spinal canal. When a fibrous scar… (more)

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In the treatment of spinal stenosis and disc herniation, the lamina and ligamentum flavum are resected to access the spinal canal. When a fibrous scar replaces the surgical defect, the dura or spinal nerves can become tethered to the surrounding tissues. Kuslich et al. and Smyth and Wright demonstrated that light mechanical stimulation of injured spinal nerves induced intraoperative or postoperative sciatic pain similar to the patients’ preoperative state [1, 2]. Therefore, adhesive peridural fibrosis contributes to the recurrence of back pain following neurosurgery. In this dissertation, biomaterial membranes are evaluated for the prevention of scar progression to the dura and spinal nerves. Cell-impermeable, biodegradable tyrosine-derived polycarbonate membranes were fabricated by electrospinning. A degradable surfactant, trans-4-hyroxy-L-proline butyl ester HCl, enabled electrospinning from a low toxicity solvent (acetic acid). Anti-adhesion membranes with a 1:1 composition of glassy and rubbery polymers were fabricated by a dual-spinneret technique, where the composite membrane’s suture retention strength, delamination strength, and toughness were greater than conventional electrospun fabrics. Controlled delivery of an antifibrotic, cis-4-hydroxy-L-proline, was achieved through synthesis of drug precursors. Hexyl- and octyl-ester precursors enabled diffusion-controlled release of the antifibrotic over 1 or more weeks, while cis-4-hydroxy-L-proline diffused from poly(DTE carbonate) films within 24 hours. Composite membranes with and without antifibrotic were compared against polymer films and Integra LifeSciences’ DuraGen PLUS® in a rat laminectomy model. The extent of adhesions was evaluated at 4 and 8 weeks. The fracture of polymeric films permitted scar progression to the dura by 8 weeks, despite inhibition at 4 weeks. The composite membranes did not fracture and reduced the extent of adhesion from 84% (control) to 36%. All adhesions in the composite membrane group formed at the caudal tuck, suggesting that performance could be improved by device fixation. Any effect of cis-4-hydroxy-L-proline was obscured by caudal instability. DuraGen PLUS® limited the extent of adhesion to 11%, and the adhesions present were comparatively light. In this dissertation, a synthetic degradable membrane was compared against polymeric films and DuraGen PLUS® in peridural adhesion prevention. The membrane possessed inherent barrier properties, superior mechanical properties, and the ability to deliver an antifibrotic. However, the device did not achieve anti-adheisve performance comparable to Integra LifeSciences’ DuraGen PLUS® in a rat laminectomy model due to the electrospun anti-adhesion membrane’s instability within the implantation site.

Advisors/Committee Members: Florek, Charles A., 1980- (author), Kohn, Joachim B (chair), Grumet, Martin (internal member), Moghe, Prabhas V (internal member), Levene, Howard B (outside member).

Subjects/Keywords: Nervous system – Surgery – Complications; Laminectomy; Electrospinning; Adhesives in surgery

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Florek, Charles A., 1. (2010). A synthetic biomaterials approach to the prevention of postsurgical adhesions in neurosurgery. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056345

Chicago Manual of Style (16^th Edition):

Florek, Charles A., 1980-. “A synthetic biomaterials approach to the prevention of postsurgical adhesions in neurosurgery.” 2010. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056345.

MLA Handbook (7^th Edition):

Florek, Charles A., 1980-. “A synthetic biomaterials approach to the prevention of postsurgical adhesions in neurosurgery.” 2010. Web. 05 Mar 2021.

Vancouver:

Florek, Charles A. 1. A synthetic biomaterials approach to the prevention of postsurgical adhesions in neurosurgery. [Internet] [Doctoral dissertation]. Rutgers University; 2010. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056345.

Council of Science Editors:

Florek, Charles A. 1. A synthetic biomaterials approach to the prevention of postsurgical adhesions in neurosurgery. [Doctoral Dissertation]. Rutgers University; 2010. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056345

Rutgers University

13. Chang, Yu-Wen. Therapeutic potential of radial glial RG3.6 cells in rat spinal cord injury.

Degree: PhD, Neuroscience, 2007, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15795

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Spinal cord injury (SCI) triggers a cascade of pathophysiological changes that lead to secondary tissue damage after the mechanical insult. Early after SCI, cells are… (more)

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Spinal cord injury (SCI) triggers a cascade of pathophysiological changes that lead to secondary tissue damage after the mechanical insult. Early after SCI, cells are disrupted and excitotoxic amino acids (e.g. glutamate) are released. Inflammatory cytokines and chemokines are quickly induced upon injury, followed by leukocyte infiltration (e.g. neutrophil and macrophage). Neurons and glia undergo massive necrotic cell death and apoptosis. Axons become progressively degenerated and extracellular substrate deposits in the lesion site, which leads to cyst and scar formation. Cell-based therapies have been widely applied during SCI sub-acute phase and at least one mechanism associated with behavioral improvement is promotion of axonal remyelination and regeneration. However, the efficacy of cell transplantation at acute SCI and associated mechanism has rarely been studied. Radial glial cells are essential in guiding and supporting neuronal migration during central nervous system development. They are neural stem cells and have trophic functions under certain conditions. In this thesis, our goal is to understand whether acute transplantation of radial glia can mitigate deleterious responses in the injured spinal cord tissue and promote functional recovery. RG3.6 is a stable neural stem cell clone derived from E13.5 cortex and has radial glial phenotypes including bipolar shape, expression of BLBP and GLAST, and support of neurite growth. Transplanted in normal spinal cord, RG3.6 retained radial glial phenotype and actively migrated along rostral-caudal axis of spinal cord and integrated nicely with host tissue. In SCI, acute transplantation of RG3.6 significantly improved locomotion recovery as early as 2 days post injury. The functional recovery may be associated with fewer infiltrating macrophages, less CSPG deposition, and more axonal preservation. At the molecular level, RG3.6 cell enhanced the expression of genes that are involved in tissue defense and stem cell development. In summary, radial glia in acute SCI modulated host responses to the injury by mediating certain gene expressions at early times, suppressed neuroinflammation, and supported and reorganized axons during later phases. Our results provide different perspectives of cell-based therapy for acute SCI.

Advisors/Committee Members: Chang, Yu-Wen (author), Grumet, Martin (chair), Firestein, Bonnie (internal member), Nowakowski, Richard (internal member), Young, Wise (internal member), Ratan, Rajiv (outside member).

Subjects/Keywords: Spinal cord – Wounds and injuries; Neuroglia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, Y. (2007). Therapeutic potential of radial glial RG3.6 cells in rat spinal cord injury. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15795

Chicago Manual of Style (16^th Edition):

Chang, Yu-Wen. “Therapeutic potential of radial glial RG3.6 cells in rat spinal cord injury.” 2007. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15795.

MLA Handbook (7^th Edition):

Chang, Yu-Wen. “Therapeutic potential of radial glial RG3.6 cells in rat spinal cord injury.” 2007. Web. 05 Mar 2021.

Vancouver:

Chang Y. Therapeutic potential of radial glial RG3.6 cells in rat spinal cord injury. [Internet] [Doctoral dissertation]. Rutgers University; 2007. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15795.

Council of Science Editors:

Chang Y. Therapeutic potential of radial glial RG3.6 cells in rat spinal cord injury. [Doctoral Dissertation]. Rutgers University; 2007. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15795

Rutgers University

14. Goff, Loyal Andrew. Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity.

Degree: PhD, Cell and Developmental Biology, 2008, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17318

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MicroRNAs represent a group of functional non-coding RNAs (ncRNA) with a role in both translational repression and/or RNAi-mediated degradation of specific target mRNAs. Reports indicate… (more)

Subjects/Keywords: Non-coding RNA; Developmental neurobiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goff, L. A. (2008). Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17318

Chicago Manual of Style (16^th Edition):

Goff, Loyal Andrew. “Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity.” 2008. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17318.

MLA Handbook (7^th Edition):

Goff, Loyal Andrew. “Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity.” 2008. Web. 05 Mar 2021.

Vancouver:

Goff LA. Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity. [Internet] [Doctoral dissertation]. Rutgers University; 2008. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17318.

Council of Science Editors:

Goff LA. Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity. [Doctoral Dissertation]. Rutgers University; 2008. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17318

Rutgers University

15. Qu, Zhao xia. Effects of erythropoietin and lithium on neural precursors and blood-derived cells.

Degree: PhD, Neuroscience, 2008, Rutgers University

URL: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17546

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Recent studies suggest that two hematopoietic drugs, erythropoietin (EPO) and lithium, can be used to treat various central nervous system (CNS) diseases, and that neural… (more)

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Recent studies suggest that two hematopoietic drugs, erythropoietin (EPO) and lithium, can be used to treat various central nervous system (CNS) diseases, and that neural precursor stimulation contributes to their beneficial effects on CNS diseases. We studied the effects of these two drugs on neural precursor cells and N01.1, a neonatal rat blood-derived cell clone generated in our laboratory, exhibiting some features of neural precursors. My experimental results suggest that EPO promotes neural precursor cell growth in vitro, and may stimulate expression of EPO receptor and neural stem cell marker nestin in rat spinal cords to benefit spinal cord injury. These studies provide important insight into the physiological functions and beneficial effects of EPO in the CNS. In addition, my studies suggest that lithium promotes proliferation but not survival of neural precursors in vitro, and the mechanism does not necessarily involve neurotrophic factors, or inositol depletion, but may involve GSK-3beta inhibition and NFAT activation. These findings not only provide mechanistic insights into the clinical effects of lithium, but also suggest an alternative therapeutic strategy for bipolar disorder and other neural diseases. EPO activated ERK pathway in N01.1 cells, which may through a c-Raf-independent mechanism. In addition, EPO tended to increase RNA levels of anti-apoptotic gene Bcl-w but not Bcl2 or Bcl-xL in N01.1 cells. These results suggest that EPO may promote N01.1 cell survival or proliferation through a mechanism different from that in erythroid precursor cells. Lithium significantly promoted cell number increase and stimulated neurotrophic factor RNA expression in N01.1 cell cultures. In addition, lithium treatment of rats that had N01.1 cells transplanted into their injured spinal cords robustly increased N01.1 cell numbers and remarkably increased RNA levels of several neurotrophic factors in the spinal cords. These results suggest a new powerful method for improving survival or proliferation of cells transplanted into the CNS, and a new method for stimulating neurotrophic factor expression in the CNS after injury.

Advisors/Committee Members: Qu, Zhao xia (author), Grumet, Martin (chair), Young, Wise (co-chair), Hart, Ronald (internal member), Hsu, Shu-Chan (internal member), Rabson, Arnold (outside member).

Subjects/Keywords: Nervous system – Regeneration; Erythropoietin – Physiological effect; Lithium – Physiological effect

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qu, Z. x. (2008). Effects of erythropoietin and lithium on neural precursors and blood-derived cells. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17546

Chicago Manual of Style (16^th Edition):

Qu, Zhao xia. “Effects of erythropoietin and lithium on neural precursors and blood-derived cells.” 2008. Doctoral Dissertation, Rutgers University. Accessed March 05, 2021. http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17546.

MLA Handbook (7^th Edition):

Qu, Zhao xia. “Effects of erythropoietin and lithium on neural precursors and blood-derived cells.” 2008. Web. 05 Mar 2021.

Vancouver:

Qu Zx. Effects of erythropoietin and lithium on neural precursors and blood-derived cells. [Internet] [Doctoral dissertation]. Rutgers University; 2008. [cited 2021 Mar 05]. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17546.

Council of Science Editors:

Qu Zx. Effects of erythropoietin and lithium on neural precursors and blood-derived cells. [Doctoral Dissertation]. Rutgers University; 2008. Available from: http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17546

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