+publisher:"Rutgers University" +contributor:("Bagnell, Carol A")

1. George, Ashley, 1991-. Maternal lactocrine programming of the porcine uterus.

Degree: PhD, Endocrinology and Animal Biosciences, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/57562/

► Nursing ensures lactocrine delivery of milk-borne bioactive factors to offspring and the lactocrine hypothesis for maternal programming proposes that disruption of lactocrine communication from birth… (more)

▼ Nursing ensures lactocrine delivery of milk-borne bioactive factors to offspring and the lactocrine hypothesis for maternal programming proposes that disruption of lactocrine communication from birth (postnatal day = PND 0) affects both the program and trajectory of porcine uterine development. Establishment of a model system for study of lactocrine-sensitive uterine organizational events shortly after birth in the neonate and for identification of potentially lactocrine-active factors found in porcine colostrum, such as insulin-like growth factor 1 (IGF1), is important. Studies show that the neonatal porcine uterine transcriptome is age- and lactocrine-sensitive on PND 2. However, whether uterine microRNA (miRNA) expression or the uterine miRNA-mRNA interactome is affected similarly is unknown. Lactocrine deficiency from birth, reflected by low serum immunoglobulin immunocrit, was associated with alterations in aspects of the neonatal uterine developmental program and, long-term, was linked to reduced lifetime fecundity in adult gilts. These observations suggested lactocrine effects on programming of adult uterine function. However, whether lactocrine deficiency and disruption of neonatal uterine development is ultimately reflected by effects on patterns of endometrial gene expression during the periattachment period of early pregnancy in adulthood is unknown. Consequently, research objectives were to: (1) determine acute effects of (a) nursing vs milk replacer feeding, and (b) method of feeding a single dose of colostrum at birth, with or without supplemental IGF1, on porcine uterine development at 12 h postnatally; (2) determine short-term effects of age and nursing on porcine uterine (a) miRNA expression between birth and PND 2 and (b) miRNA-mRNA interactions using integrated target prediction analysis; and (3) determine long-term effects of lactocrine-deficiency from birth on adult endometrial (a) mRNA and miRNA expression during the periattachment period of early pregnancy (pregnancy day 13), including identification of affected miRNA–mRNA interactions. Results showed nursing for 12 h from birth supports rapid establishment of a uterine developmental program, and that a single feeding of colostrum at birth increased endometrial cell proliferation at 12 h, regardless of method of feeding. Further, oral IGF1 was sufficient to support endometrial cell proliferation at 12 h in replacer-fed gilts, and supplementation of colostrum with IGF1 further increased endometrial cell proliferation. Between birth and PND 2, novel age- and lactocrine-sensitive uterine miRNAs and miRNA-mRNA relationships associated with porcine neonatal development were identified. On pregnancy day 13, lactocrine deficiency from birth did not affect corpora lutea number, uterine horn length, uterine wet weight, embryo recovery, or uterine luminal fluid estrogen content. However, next-generation sequencing analyses revealed lactocrine-sensitive endometrial mRNAs and miRNAs associated with aspects of solute transport, endometrial receptivity, and… Advisors/Committee Members: Bagnell, Carol A (chair), Bagnell, Carol A. (chair), Roepke, Troy A (internal member), Uzumcu, Mehmet (internal member), Roepke, Troy A. (internal member), Bartol, Frank F (outside member), Bartol, Frank F. (outside member), School of Graduate Studies.

Subjects/Keywords: Lactation

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APA (6^th Edition):

George, Ashley, 1. (2018). Maternal lactocrine programming of the porcine uterus. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/57562/

Chicago Manual of Style (16^th Edition):

George, Ashley, 1991-. “Maternal lactocrine programming of the porcine uterus.” 2018. Doctoral Dissertation, Rutgers University. Accessed January 17, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/57562/.

MLA Handbook (7^th Edition):

George, Ashley, 1991-. “Maternal lactocrine programming of the porcine uterus.” 2018. Web. 17 Jan 2021.

Vancouver:

George, Ashley 1. Maternal lactocrine programming of the porcine uterus. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Jan 17]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57562/.

Council of Science Editors:

George, Ashley 1. Maternal lactocrine programming of the porcine uterus. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57562/

Rutgers University

2. Yirtici, Seher, 1988-. Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats.

Degree: MS, Endocrinology and Animal Biosciences, 2017, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/54053/

► Endocrine-disrupting chemicals (EDCs) are synthetic or natural compounds that can be found in the environment in various forms, such as pesticides [e.g., methoxychlor (MXC)], plasticizers… (more)

▼ Endocrine-disrupting chemicals (EDCs) are synthetic or natural compounds that can be found in the environment in various forms, such as pesticides [e.g., methoxychlor (MXC)], plasticizers [e.g., bisphenol A (BPA)], or pharmaceutical agents [e.g., diethylstilbestrol (DES), a potent synthetic estrogen that was prescribed to pregnant women between 1940s and 1970s. Previous studies indicated that the ovary is particularly vulnerable to estrogenic EDC exposures during fetal and neonatal periods. To investigate the effects of estrogenic EDCs in two critical developmental windows, we performed two experiments, using timed-pregnant Fisher CDF rats. In Experiment 1, we exposed the dams to 50 µg/kg/day (Low) BPA or 50 mg/kg/day (High) BPA, or vehicle from embryonic day (E) 18 to 21, and the pups from postnatal day (PND) 0 to 7. In Experiment 2, we exposed animals to 0.1µg/kg/day DES, 75 mg/kg/day MXC, 50 mg/kg/day BPA, or vehicle from E11 to PND7. We then examined reproductive parameters, including pubertal age, regularity of reproductive cycles, and follicular composition. Expression of critical ovarian markers, including estrogen receptor 1 (ESR1), Mullerian inhibiting substance (MIS), cytochrome P450 side chain cleavage (P450scc), luteinizing hormone receptor (LHR), and proliferating cell nuclear antigen (PCNA) were also examined. In Experiment 1, we found that there were no changes in age at puberty or estrous cyclicity in the both Low and High BPA-treated females. However, there was a decrease (p < 0.05) in primary follicles in High BPA-treated rats. In addition, there was an increase in atretic follicle numbers (p < 0.05) in the Low and High BPA-treated females suggesting that there was an effect on follicular dynamics. Moreover, a short window of ovarian exposure to BPA did not show any statistically significant effects on expression of ovarian markers except ESR1which was decreased in primary follicles in Low BPA-treated females. In Experiment 2, our results demonstrated that DES- and MXC-treated females had an accelerated onset of puberty and altered estrous cyclicity. Although there was no effect on the litter size, MXC-treated females showed a strong trend towards reduction in litter size (p = 0.07) as compared to control. MXC caused a decrease in steroid hormone levels. DES, MXC, and BPA exposures altered follicular dynamics. There was an increase in atretic follicles in DES and BPA-treated females. In addition, the number of corpora lutea (CL) was reduced (p < 0.01) in MXC-treated females while the total follicle numbers were not altered. There were alterations in ovarian molecular markers. Expression of MIS significantly increased in secondary and pre-antral follicles in MXC-treated group. Furthermore, expression of P450scc decreased in pre- and early-antral follicles in MXC-treated females while the expression increased in CL in BPA-treated females. Overall, the results show that developmental exposure to estrogenic-EDCs affects female reproduction and ovarian follicular dynamics. In addition, DES and MXC… Advisors/Committee Members: Uzumcu, Mehmet (chair), Zama, Aparna M. (internal member), Bagnell, Carol A. (internal member), Roepke, Troy A. (internal member).

Subjects/Keywords: Pisphenol A – Toxicity; Plastics – Toxicology; Plastics – Environmental aspects; Endocrine disrupting chemicals

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yirtici, Seher, 1. (2017). Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/54053/

Chicago Manual of Style (16^th Edition):

Yirtici, Seher, 1988-. “Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats.” 2017. Masters Thesis, Rutgers University. Accessed January 17, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/54053/.

MLA Handbook (7^th Edition):

Yirtici, Seher, 1988-. “Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats.” 2017. Web. 17 Jan 2021.

Vancouver:

Yirtici, Seher 1. Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats. [Internet] [Masters thesis]. Rutgers University; 2017. [cited 2021 Jan 17]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/54053/.

Council of Science Editors:

Yirtici, Seher 1. Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats. [Masters Thesis]. Rutgers University; 2017. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/54053/

Rutgers University

3. Camp, Meredith E., 1985-. Lactocrine signaling and neonatal porcine reproductive tract development.

Degree: PhD, Animal Sciences, 2015, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/46315/

►

Nursing supports neonatal porcine reproductive tract development by delivering milk-borne bioactive factors (MbFs) from mother to offspring as proposed in the lactocrine hypothesis. In pigs,… (more)

▼

Nursing supports neonatal porcine reproductive tract development by delivering milk-borne bioactive factors (MbFs) from mother to offspring as proposed in the lactocrine hypothesis. In pigs, studies showed that age and nursing support postnatal uterine and testicular development between birth [postnatal day (PND) 0] and PND 2 via a lactocrine mechanism and define the developmental program. However, age-sensitive events associated with development of the neonatal porcine uterine transcriptome have not been defined during this period. Additionally, the extent to which nursing affects the development of the porcine cervix is unknown. Furthermore, the extent to which MbFs, particularly insulin-like growth factor-1 (IGF1), affect development of the porcine cervix remains to be determined. Research aims were to (1) define the age-sensitive uterine transcriptome in gilts at birth compared to animals at PND 2; (2) determine the effects of age and nursing on porcine cervical histoarchitecture and cell proliferation; and (3) determine whether a single feeding of colostrum or milk replacer at birth, with or without oral IGF1 supplementation, supports cervical cell proliferation and development using a new 12 h neonatal pig bioassay. Results showed that in uteri of PND 2 as compared to PND 0 gilts, 3283 genes were differentially expressed and multiple age-sensitive biological processes and pathways, including ‘immune response’ and ‘Wnt-β-catenin signaling’, were affected. Additionally, results indicated that both age and nursing supported cervical development histologically by PND 14 and cell proliferation by PND 2 and 14. Furthermore, oral IGF1 increased cervical cell proliferation when administered in milk replacer, but not with colostrum. However, IGF1 supplementation in either colostrum or milk replacer increased cervical IGF1 signaling cascade markers B-cell lymphoma 2 and phospho-AKT compared to gilts fed colostrum or milk replacer alone. In conclusion, the global changes in uterine gene expression identified here within the first 48 h after birth provide a foundation for future studies to better understand the mechanisms and pathways governing FRT development. Collectively, results presented here reinforce and extend previous findings that both age and lactocrine signaling are effectors of organizationally critical structural changes in developing female reproductive tissues with potential to determine reproductive capacity in adulthood.

Advisors/Committee Members: Bagnell, Carol A (chair), Cohick, Wendie S (internal member), Pietrzykowski, Andre (internal member), Bartol, Frank F (outside member).

Subjects/Keywords: Colostrum; Milk – Composition; Swine – Fetuses

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Camp, Meredith E., 1. (2015). Lactocrine signaling and neonatal porcine reproductive tract development. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/46315/

Chicago Manual of Style (16^th Edition):

Camp, Meredith E., 1985-. “Lactocrine signaling and neonatal porcine reproductive tract development.” 2015. Doctoral Dissertation, Rutgers University. Accessed January 17, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/46315/.

MLA Handbook (7^th Edition):

Camp, Meredith E., 1985-. “Lactocrine signaling and neonatal porcine reproductive tract development.” 2015. Web. 17 Jan 2021.

Vancouver:

Camp, Meredith E. 1. Lactocrine signaling and neonatal porcine reproductive tract development. [Internet] [Doctoral dissertation]. Rutgers University; 2015. [cited 2021 Jan 17]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46315/.

Council of Science Editors:

Camp, Meredith E. 1. Lactocrine signaling and neonatal porcine reproductive tract development. [Doctoral Dissertation]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/46315/

Rutgers University

4. Yasrebi, Ali, 1987-. ERE-independent ERα signalling in feeding and exploratory behaviors.

Degree: MS, Endocrinology and Animal Biosciences, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/62067/

► The reproductive steroid hormone, 17β-estradiol (E2), controls feeding and exploratory behaviors associated with mood disorders. The loss of circulating E2 puts menopausal women at an… (more)

▼ The reproductive steroid hormone, 17β-estradiol (E2), controls feeding and exploratory behaviors associated with mood disorders. The loss of circulating E2 puts menopausal women at an increased risk for developing obesity and mood disorders when compared to premenopausal women. Therefore, it is critically important to understand the role of sex steroids and their receptors in the neuroendocrine control of feeding and mood. The goal of this project is to understand the role of estrogen response Element (ERE)-dependent and ERE-independent ERα signaling on behavior by characterizing feeding patters and exploratory behaviors in male and female mice lacking either total ERα signaling or lacking ERE-dependent ERα signaling. We hypothesize that ERE-independent ERα is partially sufficient to restore feeding and exploratory behaviors that are lost in total ERα knockout mice. We tested three strains of mice: two ERα transgenic models, a total ERα knock out (ERKO) and a novel ERα knock in/knock out (KIKO) that lacks a functional DNA-binding domain) and their wild type (WT) C57 littermates using a real-time feeding behavior monitoring system and series of standard behavior tests (open field tests, elevated plus maze, forced swim test). To test our hypothesis FI and meal patterns were observed while the animals were given ad libitum access to a LFD (Experiment 1a) followed by HFD (Experiment 1b). A separate set of animals the response to fasting was monitored for 24 h after caloric restriction (Experiment 1c). Exploratory, depressive, and locomotor behavior testing was conducted on mice from Experiments 1a/b (open field, elevated plus maze, forced swim test). Each experiment was initially done with intact animals and then again repeated in ovariectomized (OVX) animals split into either an oil treated control group or an E2-treated group. We observed ERE-dependent mechanisms are the main modulator of homeostatic LFD feeding meal patterns while ERE- independent ERα signaling was involved in the control of palatable, high-fat diet food intake. During refeeding, ERE-independent mechanisms contribute to a decreased first meal food intake and slower rate of ingestion. When observed during a series of behavior tests, WT animals explored more, regardless of treatment (differences could be attributed to higher levels of locomotor activity in WT). However, similarities between WT and KIKO females in the EPM indicate that ERE-independent pathways may contribute towards reducing anxiety measures, independent of locomotor activity. WT females were shown to have a decreased free float time, indicating ERE dependent signaling may be influencing despair like tendencies. Collectively, these suggest that both ERE-dependent and -independent ERαsignaling are involved with both feeding and anxiety like homeostatic parameters. Advisors/Committee Members: Roepke, Troy A (chair), Bello, Nicholas T (internal member), Bagnell, Carol A (internal member), Samuels, Benjamin A (outside member), School of Graduate Studies.

Subjects/Keywords: Estrogen receptor alpha; Estrogen – Receptors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yasrebi, Ali, 1. (2019). ERE-independent ERα signalling in feeding and exploratory behaviors. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/62067/

Chicago Manual of Style (16^th Edition):

Yasrebi, Ali, 1987-. “ERE-independent ERα signalling in feeding and exploratory behaviors.” 2019. Masters Thesis, Rutgers University. Accessed January 17, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/62067/.

MLA Handbook (7^th Edition):

Yasrebi, Ali, 1987-. “ERE-independent ERα signalling in feeding and exploratory behaviors.” 2019. Web. 17 Jan 2021.

Vancouver:

Yasrebi, Ali 1. ERE-independent ERα signalling in feeding and exploratory behaviors. [Internet] [Masters thesis]. Rutgers University; 2019. [cited 2021 Jan 17]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/62067/.

Council of Science Editors:

Yasrebi, Ali 1. ERE-independent ERα signalling in feeding and exploratory behaviors. [Masters Thesis]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/62067/

Rutgers University

5. Frankshun, Amy-Lynn, 1982-. The role of nursing on maternal programming of the neonatal porcine cervix.

Degree: PhD, Animal Sciences, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057719

►

Nutritional and immunological benefits of colostrum for neonatal growth and survival are well known. However, colostrum’s role as a conduit for bioactive factors that can… (more)

▼

Nutritional and immunological benefits of colostrum for neonatal growth and survival are well known. However, colostrum’s role as a conduit for bioactive factors that can affect neonatal development is not well understood. The lactocrine hypothesis for maternal programming of neonatal development was proposed as a mechanism whereby milk-borne agents, including the prototypical hormone relaxin (RLX), are delivered to nursing offspring to affect target tissues. Research goals were to understand how milk-borne agents, like RLX, affect neonatal porcine cervical development, by altering mediators of growth (estrogen receptor-alpha [ESR1], vascular endothelial growth factor [VEGFA] and the RLX receptor [RXFP1]), connective tissue remodeling (matrix metalloproteinase 2 [MMP2]) and apoptosis (anti-apoptotic BCL2 and pro-apoptotic cleaved caspase 3). Results showed biologically active proRLX in porcine milk during the first two weeks of lactation. Nursing for two days from birth was important for cervical ESR1, VEGFA and BCL2 protein expression, undetectable at postnatal day (PND) 0 and in replacer-fed gilts over the same period. Exogenous RLX failed to restore the cervical phenotype of replacer-fed animals to that of nursing gilts at PND 2. The extent to which this two day window of sensitivity influences subsequent cervical development at PND 14 was investigated. Cervical ESR1 and BCL2 proteins were detected in nursed gilts, but not in gilts fed replacer from birth through PND 14. VEGFA protein was detectable on PND 14 in both nursed and replacer-fed gilts, but was markedly reduced in PND 14 gilts fed replacer from birth. Active MMP2 was detected in both nursed and replacer-fed gilts by PND 14. Data support the lactocrine hypothesis, indicating that milk-borne factors are required to support protein expression patterns important for cervical development in the neonatal gilt. Results indicate that the first two days of life constitute a critical period for lactocrine signaling in porcine cervical tissues. Effects of disruption of lactocrine signaling on cervical development between birth and PND 2 persisted to PND 14 even when replacer-fed gilts returned to nursing after PND 2. If animals are colostrum-deprived, altered expression of morphoregulatory proteins could affect neonatal cervical development with long-term consequences for reproductive performance and health.

Advisors/Committee Members: Frankshun, Amy-Lynn, 1982- (author), Bagnell, Carol A (chair), John-Alder, Henry (internal member), Cohick, Wendie (internal member), Katz, Larry (internal member), Steinetz, Bernard (outside member).

Subjects/Keywords: Colostrum – Research; Newborn infants – Development; Cervix uteri; Swine as laboratory animals; Relaxin; Breastfeeding

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frankshun, Amy-Lynn, 1. (2011). The role of nursing on maternal programming of the neonatal porcine cervix. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057719

Chicago Manual of Style (16^th Edition):

Frankshun, Amy-Lynn, 1982-. “The role of nursing on maternal programming of the neonatal porcine cervix.” 2011. Doctoral Dissertation, Rutgers University. Accessed January 17, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057719.

MLA Handbook (7^th Edition):

Frankshun, Amy-Lynn, 1982-. “The role of nursing on maternal programming of the neonatal porcine cervix.” 2011. Web. 17 Jan 2021.

Vancouver:

Frankshun, Amy-Lynn 1. The role of nursing on maternal programming of the neonatal porcine cervix. [Internet] [Doctoral dissertation]. Rutgers University; 2011. [cited 2021 Jan 17]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057719.

Council of Science Editors:

Frankshun, Amy-Lynn 1. The role of nursing on maternal programming of the neonatal porcine cervix. [Doctoral Dissertation]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000057719

Rutgers University

6. Rahman, Kathleen M., 1986-. Milk-borne bioactive factors: effects on neonatal porcine reproductive tissues.

Degree: Animal Sciences, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/44200/

Subjects/Keywords: Milk – Composition; Testis; Swine – Development – Endocrine aspects; Biotransformation (Metabolism)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rahman, Kathleen M., 1. (2014). Milk-borne bioactive factors: effects on neonatal porcine reproductive tissues. (Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/44200/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rahman, Kathleen M., 1986-. “Milk-borne bioactive factors: effects on neonatal porcine reproductive tissues.” 2014. Thesis, Rutgers University. Accessed January 17, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/44200/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rahman, Kathleen M., 1986-. “Milk-borne bioactive factors: effects on neonatal porcine reproductive tissues.” 2014. Web. 17 Jan 2021.

Vancouver:

Rahman, Kathleen M. 1. Milk-borne bioactive factors: effects on neonatal porcine reproductive tissues. [Internet] [Thesis]. Rutgers University; 2014. [cited 2021 Jan 17]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/44200/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rahman, Kathleen M. 1. Milk-borne bioactive factors: effects on neonatal porcine reproductive tissues. [Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/44200/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

7. Chen, Joseph C., 1978-. Factors that define the developmental program and trajectory of the porcine uterus.

Degree: PhD, Animal Sciences, 2010, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056212

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The early days of life represent an important period for neonatal uterine development in the pig. The porcine uterus develops in utero and continues postnatally.… (more)

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The early days of life represent an important period for neonatal uterine development in the pig. The porcine uterus develops in utero and continues postnatally. Events during neonatal life that establish uterine histoarchitecture contribute to the developmental program of the uterus and set the trajectory for adult uterine phenotype. Uterine programming is sensitive to hormonal perturbations. Exposure to bioactive factors during neonatal life influences uterine maturation with long-term consequences for reproductive performance. The goal of this research was to study factors in the neonatal environment that alter uterine developmental gene expression. These include mediators of growth [estrogen receptor alpha (ESR1), vascular endothelial growth factor (VEGFA) and relaxin receptor (RXFP1)], patterning (WNT7A and HOXA10) and remodeling [matrix metalloproteinase (MMP)9 and 2]. Results indicated that exposure to estradiol valerate (EV) from birth (postnatal day [PND] 0) to PND 14 increased uterine ESR1 and VEGFA protein, along with HOXA10, RXFP1 and MMP9 transcripts and decreased WNT7A transcripts, in PND 14 neonates. Endometrial gene expression changes also occured in adulthood on day 12 of pregnancy as a consequence of neonatal EV exposure. This included decreases in WNT7A and MMP9 transcripts, ESR1 and VEGFA protein and reduced MMP9 activity. Next, exposing gilts perinatally to the estrogenic mycotoxin zearalenone (ZEA), decreased uterine ESR1, WNT7A and RXFP1 mRNA levels on PND 21 compared to unexposed gilts. Extending the concept that postnatal uterine development in gilts can be influenced by maternally-derived signals, studies showed that nursing from birth supported uterine ESR1, VEGFA and MMP9 protein expression at PND 2 and PND 14 in comparison to milk replacer-fed gilts. Treatment with relaxin, an uterotrophic milk-borne hormone, did not restore the uterine phenotype of replacer-fed animals to that of nursing gilts at PND 2, although it did predictably affect RXFP1 transcript levels. Results support the idea that nursing during a critical two-day period from birth supports the neonatal porcine uterine developmental program. Identification of mediators that regulate neonatal uterine programming, the time frame for this regulation and understanding how those mediators are influenced by environmental/lactocrine factors provide critical insights into the mechanisms that govern neonatal tissue development.

Advisors/Committee Members: Chen, Joseph C., 1978- (author), Bagnell, Carol A. (chair), Katz, Larry S. (internal member), Uzumcu, Mehmet (internal member), Bartol, Frank F. (outside member).

Subjects/Keywords: Swine – Growth; Uterus – Growth; Gene expression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, Joseph C., 1. (2010). Factors that define the developmental program and trajectory of the porcine uterus. (Doctoral Dissertation). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056212

Chicago Manual of Style (16^th Edition):

Chen, Joseph C., 1978-. “Factors that define the developmental program and trajectory of the porcine uterus.” 2010. Doctoral Dissertation, Rutgers University. Accessed January 17, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056212.

MLA Handbook (7^th Edition):

Chen, Joseph C., 1978-. “Factors that define the developmental program and trajectory of the porcine uterus.” 2010. Web. 17 Jan 2021.

Vancouver:

Chen, Joseph C. 1. Factors that define the developmental program and trajectory of the porcine uterus. [Internet] [Doctoral dissertation]. Rutgers University; 2010. [cited 2021 Jan 17]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056212.

Council of Science Editors:

Chen, Joseph C. 1. Factors that define the developmental program and trajectory of the porcine uterus. [Doctoral Dissertation]. Rutgers University; 2010. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056212

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