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Purdue University

1. Dabral, Neha. NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE.

Degree: PhD, Comparative Pathobiology, 2014, Purdue University

The genus Brucella consists of Gram-negative, facultative intracellular coccobacilli that can cause chronic infections in several mammals. Brucella spp. can exhibit a smooth or rough phenotype; smooth Brucella spp. contain a surface-exposed O-polysaccharide in their cell wall structure while the rough Brucella spp. are devoid of the O-polysaccharide. Acquired immunity against Brucella infection is primarily cell-mediated and involves both CD4+ T cells and CD8+ T cell responses. However, antibodies to the O-polysaccharide also play a role in enhancing the protection against infections by virulent Brucella species in some hosts. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis in the United States and several other countries. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide in Brucella, is disrupted in B. abortus RB51 by an IS711 element. Although low-levels of intra-cytoplasmic O-polysaccharide were produced when RB51 was complemented with a functional wboA gene (strain RB51WboA), it did not result in a smooth phenotype. This suggests that mutations in several genes of the O-polysaccharide biosynthesis pathway contribute to the rough phenotype of RB51. However, nucleotide sequence analysis has revealed that there are no other gene-disrupting mutations that could affect the smooth LPS synthesis in strain RB51. Advisors/Committee Members: Ramesh Vemulapalli, Harm HogenEsch, Carolyn Guptill-Yoran, Mohamed Seleem.

Subjects/Keywords: Brucella; Exopolysaccharide; Gamma irradiation; Overexpression; Prime-boost; Vaccine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Dabral, N. (2014). NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE. (Doctoral Dissertation). Purdue University. Retrieved from https://docs.lib.purdue.edu/open_access_dissertations/1071

Chicago Manual of Style (16th Edition):

Dabral, Neha. “NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE.” 2014. Doctoral Dissertation, Purdue University. Accessed February 18, 2020. https://docs.lib.purdue.edu/open_access_dissertations/1071.

MLA Handbook (7th Edition):

Dabral, Neha. “NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE.” 2014. Web. 18 Feb 2020.

Vancouver:

Dabral N. NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE. [Internet] [Doctoral dissertation]. Purdue University; 2014. [cited 2020 Feb 18]. Available from: https://docs.lib.purdue.edu/open_access_dissertations/1071.

Council of Science Editors:

Dabral N. NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE. [Doctoral Dissertation]. Purdue University; 2014. Available from: https://docs.lib.purdue.edu/open_access_dissertations/1071

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