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You searched for +publisher:"Penn State University" +contributor:("Jin Ming Yang, Dissertation Advisor/Co-Advisor"). One record found.

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Penn State University

1. Huber-keener, Kathryn Joyce. Alterations In Gene Expression As A Response of Tumor Cells To Stresses .

Degree: 2013, Penn State University

Cancer cells are constantly under stress, a factor which needs to be taken into consideration during the treatment of cancers. Both intrinsic and extrinsic stresses impact the development and progression of neoplasms, down to the level of individual proteins and genes. Stresses like nutrient deficiency, hypoxia, and the immune response are present during normal tumor growth and throughout treatment. Tumor cells that survive these stresses are more adept at surviving hostile conditions and are more resistant to current therapies. Stress, therefore, shapes the tumor cell population. Gene expression alterations are the driving feature behind the adaptive ability of cancer cells to these stresses, and therefore, careful examination of these gene expression changes must be undertaken in order to develop effective therapies. In the present investigations, we first explore the global alterations of gene expression in tamoxifen resistance. Resistance to tamoxifen (Tam), a widely used antagonist of the estrogen receptor (ER), occurs in one third of patients treated with Tam within 5 years of therapy. A better understanding of gene expression alterations associated with Tam resistance will facilitate circumventing this problem. Using a next generation sequencing approach and a new bioinformatics model, we compared the transcriptomes of Tam-sensitive and Tam-resistant breast cancer cells for identification of genes involved in the development of Tam resistance. We identified differential expression of 1215 mRNA and 513 small RNA transcripts clustered into ERĪ± functions, cell cycle regulation, gen expression, and mitochondrial modification. The extent of alterations found at multiple levels of gene regulation highlights the ability of the Tam-resistant cells to modulate global gene expression. Alterations of small nucleolar RNA, oxidative phosphorylation, and a protein called SIRT3 in Tam-resistant cells present areas for diagnostic and therapeutic tool development for combating resistance to this anti-estrogen agent. After such a global exploration of cancer cell responses to stress, we next investigated a mechanism of cancer cell survival by exploring the alterations in protein synthesis inhibitor eEF-2K. Studies show that eEF-2K plays a role in cell survival through this inhibition of protein synthesis and that its protein levels are increased in cancer. Post-translational modification of translation machinery is important for its regulation and could be critical for survival of cancer cells encountering stress. Thus, the purpose of our study is to examine the regulation of eEF-2K during stress with a focus on the phosphorylation status and stability of EF-2K protein in cancer cells. Using two human glioma cell lines (T98G and LN229), under stress conditions, we found a paradoxical increase in eEF-2K protein expression with a decrease in eEF-2K protein stability. Phosphorylation may play a role in this altered protein stability as EF-2K has multiple phosphorylation sites that are phosphorylated by the… Advisors/Committee Members: Jin Ming Yang, Dissertation Advisor/Co-Advisor, Robert G Levenson, Committee Member, Rongling Wu, Committee Member, Willard Freeman, Committee Member, Andrea Manni, Committee Member.

Subjects/Keywords: eEF-2K; tamoxifen; glioma; breast cancer; protein synthesis; next generation-sequencing; protein stability

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APA (6th Edition):

Huber-keener, K. J. (2013). Alterations In Gene Expression As A Response of Tumor Cells To Stresses . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/15352

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Huber-keener, Kathryn Joyce. “Alterations In Gene Expression As A Response of Tumor Cells To Stresses .” 2013. Thesis, Penn State University. Accessed March 03, 2021. https://submit-etda.libraries.psu.edu/catalog/15352.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Huber-keener, Kathryn Joyce. “Alterations In Gene Expression As A Response of Tumor Cells To Stresses .” 2013. Web. 03 Mar 2021.

Vancouver:

Huber-keener KJ. Alterations In Gene Expression As A Response of Tumor Cells To Stresses . [Internet] [Thesis]. Penn State University; 2013. [cited 2021 Mar 03]. Available from: https://submit-etda.libraries.psu.edu/catalog/15352.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huber-keener KJ. Alterations In Gene Expression As A Response of Tumor Cells To Stresses . [Thesis]. Penn State University; 2013. Available from: https://submit-etda.libraries.psu.edu/catalog/15352

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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