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You searched for +publisher:"Penn State University" +contributor:("Dr Juliette Lecomte, Committee Member"). One record found.

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Penn State University

1. Heinnickel, Mark Lewis. STUDY OF THE IRON SULFUR CLUSTER AND POLYPEPTIDE COMPOSITION OF THE PHOTOSYNTHETIC REACTION CENTER FROM HELIOBACTERIUM MODESTICALDUM.

Degree: 2008, Penn State University

The photosynthetic reaction center from Heliobacterium modesticaldum (HbRC) was studied using a variety of biochemical and biophysical techniques. The purpose of the work was to obtain a better understanding of the electron transfer cofactors and polypeptides that comprise the HbRC. The major findings include: (1) the HbRC loosely binds a ferredoxin-like protein that harbors two iron-sulfur clusters. This protein, which dissociates from the HbRC when it is passed over an anion exchange resin, was named PshB. HbRC complexes purified in this manner are henceforth referred to as HbRC cores. PshB can also be removed from HbRC complexes at high ionic strength and purified by passage over an ultrafiltration membrane. PshB was shown to restore the light-induced EPR signal of [FA/FB]- (visible at low temperatures), and the characteristic flash-induced kinetic decay of P798+ when recombined with HbRC cores. (2) HbRC cores bind an interpolypeptide iron sulfur cluster termed FX. When HbRC cores are incubated with sodium dithionite in the presence of light, FX undergoes a one-electron reduction. Mossbauer and EPR data indicate the reduced cluster has a ground spin state of S=3/2. (3) Analysis of the non-heme iron content shows that HbRC cores bind 21.1 ± 1.1 Bchl g/P798. (4) The PshB protein was cloned from H. modesticaldum using knowledge of the N-terminal sequence of Fd2, a ferredoxin that had been isolated previously from Heliobacillis mobilis. When Fd2 is overexpressed in Escherichia coli with a His affinity tag and reconstituted with iron and sulfide, the holoprotein was found to co-purify with HbRC cores when subject to G-75 gel filtration chromatography and Ni-affinity chromatography. Similar to PshB from H. modesticaldum, the overexpressed Fd2 holoprotein restores the light-induced EPR signal of [FA/FB]- and the characteristic flash-induced kinetic decay of P798+ when recombined with HbRC cores. This work indicates the function of Fd2 is now properly assigned: instead of representing a soluble ferredoxin, Fd2 is PshB and is normally bound to the HbRC complex. Advisors/Committee Members: John H Golbeck, Committee Chair/Co-Chair, Squire J Booker, Committee Member, Joseph M Bollinger Jr., Committee Member, Donald Ashley Bryant, Committee Member, Dr Juliette Lecomte, Committee Member.

Subjects/Keywords: Heliobacterium; iron sulfur clusters; EPR; photosynthesis; anaerobic

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APA (6th Edition):

Heinnickel, M. L. (2008). STUDY OF THE IRON SULFUR CLUSTER AND POLYPEPTIDE COMPOSITION OF THE PHOTOSYNTHETIC REACTION CENTER FROM HELIOBACTERIUM MODESTICALDUM. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/7567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Heinnickel, Mark Lewis. “STUDY OF THE IRON SULFUR CLUSTER AND POLYPEPTIDE COMPOSITION OF THE PHOTOSYNTHETIC REACTION CENTER FROM HELIOBACTERIUM MODESTICALDUM.” 2008. Thesis, Penn State University. Accessed November 29, 2020. https://submit-etda.libraries.psu.edu/catalog/7567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Heinnickel, Mark Lewis. “STUDY OF THE IRON SULFUR CLUSTER AND POLYPEPTIDE COMPOSITION OF THE PHOTOSYNTHETIC REACTION CENTER FROM HELIOBACTERIUM MODESTICALDUM.” 2008. Web. 29 Nov 2020.

Vancouver:

Heinnickel ML. STUDY OF THE IRON SULFUR CLUSTER AND POLYPEPTIDE COMPOSITION OF THE PHOTOSYNTHETIC REACTION CENTER FROM HELIOBACTERIUM MODESTICALDUM. [Internet] [Thesis]. Penn State University; 2008. [cited 2020 Nov 29]. Available from: https://submit-etda.libraries.psu.edu/catalog/7567.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Heinnickel ML. STUDY OF THE IRON SULFUR CLUSTER AND POLYPEPTIDE COMPOSITION OF THE PHOTOSYNTHETIC REACTION CENTER FROM HELIOBACTERIUM MODESTICALDUM. [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/7567

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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