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You searched for +publisher:"NSYSU" +contributor:("Song-Tay Lee"). One record found.

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NSYSU

1. Lee, Meng-Yao. Expression of Fractalkine chemokine domain by baculovirus expression system.

Degree: Master, Biological Sciences, 2013, NSYSU

Baculovirus expression system has often been utilized to express recombinant proteins.The advantages of this system include high efficiency, ability to express proteins in manytypes of cells, and production of proteins with correct conformation and posttranslational modifications. Fractalkine is a membrane protein mainly exerting its effects through binding of extracellular chemokine domain to the CX3CR1 receptor. It regulates proliferation, differentiation, and apoptosis of cells. It also plays an important role in cardiovascular diseases. The goal of this thesis is to construct a baculovirus vector system to express fractalkine chemokine domain for further functional studies. The vector system is engineered to contain a secretion signal peptide sequence and two affinity tags, polyhistidine tag for purification and myc tag for immunoblotting. Using this system, it was found that fractalkine chemokine domain is able to express in HEK293 and HeLa cells, whereas THP-1 monocyte and Jurkat T cells are without effect. The expressed recombinant protein could be detected at 60 h after viral infection and the majority appeared to be produced after 72 h. In addition, the fractalkine chemokine domain produced in HEK293 cells was able to purified from the medium through polyhistidine tag pulldown. These results indicate that a highly efficient protein expression system with advantages of easy detection and purification has been established and this system is a valuable tool in structural and functional studies of proteins. Advisors/Committee Members: Jong-Kang Liu (committee member), Jin-Ching Lee (chair), Song-Tay Lee (chair), Hung Wu (committee member).

Subjects/Keywords: Fractalkine; His tag; baculovirus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lee, M. (2013). Expression of Fractalkine chemokine domain by baculovirus expression system. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Lee, Meng-Yao. “Expression of Fractalkine chemokine domain by baculovirus expression system.” 2013. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Lee, Meng-Yao. “Expression of Fractalkine chemokine domain by baculovirus expression system.” 2013. Web. 15 Jan 2021.

Vancouver:

Lee M. Expression of Fractalkine chemokine domain by baculovirus expression system. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee M. Expression of Fractalkine chemokine domain by baculovirus expression system. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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