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You searched for +publisher:"NSYSU" +contributor:("Sheu, Jim Jinn-Chyuan"). One record found.

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NSYSU

1. Shen, Ying-Ying. Generation and Characterization of Recombinant Adenovirus Encoding Irisin.

Degree: Master, Institute of Biomedical Sciences, 2017, NSYSU

Exercise represents one of the most effective approaches for control of obesity and metabolic syndromes. Irisin is a 112-residue myokine secreted by skeletal muscle through proteolytical cleavage from its precursor fibronectin type III domain containing 5 (FNDC5). Irisin stimulates brown fat-like development from white fat and thermogenesis through increasing mitochondria genesis and energy expenditure, thereby reducing body weight and insulin resistance. Because of its anti-obesity effects and evolutionary conservation, Irisin has been proposed as a promising therapeutic agent for metabolic diseases since its discovery in 2012. To combat the obesity syndrome, gene therapy approached may be required for long-term management of patients with metabolic diseases. Thus, the present study aims to generate the recombinant adenovirus vectors for Irisin production in various types of cells/organs, thereby evaluating their therapeutic potential and mechanism. Recombinant irisin was expressed and purified from E. coli with an apparent molecular weight of 14 kDa. The anti-irisin antibody was raised by periodical injection of recombinant irisin into rabbit and purified from serum using protein G Sepharose affinity chromatography. For gene delivery, adenovirus vector encoding FNDC5 (Ad-FNDC5) and irisin (Ad-irisin) were generated and purified by cesium chloride ultracentrifugation. To investigate the profile of FNDC5/irisin expression in different types of cells, endothelial EA.hy926, muscle C2C12, hepatocytes Clone-9, and embryonic kidney HEK293 cells were employed for adenovirus gene delivery. By immunoblot analysis and enzyme-linked immunoassay (ELISA), it was found that Ad-irisin effectively transduced and conferred irisin secretion in all four types of cells whereas Ad-FNDC5 evoked moderate irisin secretion only in C2C12 and Clone-9 cells. Infection with Ad-irisin enhanced the viability and migration of endothelial cells, supporting the pro-angiogenic function of irisin. Besides, Ad-irisin-infected hepatocytes exhibited elevated activities of AMPK/Akt and inhibition of PEPCK signaling, suggesting the role of irisin in gluconeogenesis in the livers. With the development of these irisin-based tools, future studies are warranted to elucidate the cellular function of irisin in different organs, thereby exploring the therapeutic potential of irisin therapy for various human diseases. Advisors/Committee Members: Sheu, Jim Jinn-Chyuan (chair), Chen, Chun-Lin (chair), Tai, Ming-Hong (committee member).

Subjects/Keywords: angiogenesis; gene therapy; obesity; myokines; Irisin; gluconeogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Shen, Y. (2017). Generation and Characterization of Recombinant Adenovirus Encoding Irisin. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Shen, Ying-Ying. “Generation and Characterization of Recombinant Adenovirus Encoding Irisin.” 2017. Thesis, NSYSU. Accessed April 16, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Shen, Ying-Ying. “Generation and Characterization of Recombinant Adenovirus Encoding Irisin.” 2017. Web. 16 Apr 2021.

Vancouver:

Shen Y. Generation and Characterization of Recombinant Adenovirus Encoding Irisin. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Apr 16]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shen Y. Generation and Characterization of Recombinant Adenovirus Encoding Irisin. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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