The protective effects of curcumin on the pancreatic juice induced lung injury.
Degree: PhD, Biological Sciences, 2015, NSYSU
Emerging diseases such as middle east respiratory syndrome, severe acute respiratory syndrome or other infections induced by Ebola virus, Bacillus anthracis, and Legionnaireâs disease which are all related to lung inflammation and lung edema with high mortality. The mechanisms involved in these emerging diseases could be related to the microbes infection and the inflammation reactions involved in the lung. The inflammation of the lung could be induced by sequestered of the inflammatory cells in the lung, so that oxidative stress increased, pulmonary arterial pressure increased, and the permeability changes in pulmonary capillaries. Besides, blood cells sequestered in the lung-induced ischemia/ reperfusion (I/R) of lung tissues are also related. Necrosis of intestines or inflammation of pancreas also could induce systemic inflammatory responses, so that hemodynamic changes in the lung, pulmonary capillary permeability changes and lung edema ensured.
In the pilot study we proved that ischemia/ reperfusion of the lung tissues induced lung water increase (p < 0.01), lavage protein in pulmonary capillary bronchial alveolar lavage (PCBAL) increase (p < 0.001) and the permeability coefficient of pulmonary capillary increase (p < 0.05). After administration of water soluble oxygen radical scavenger, dimethylthiourea, the lung injury parameters attenuated significantly (PCBAL, p < 0.01; LW/BW, p < 0.05). However, big molecules of oxygen radical scavengers such as superoxide dismutase and catalase did not prove with protective effects (p > 0.05). These could be related to the big molecules of superoxide dismutase and catalase which canât penetrate into cells.
Further experiment, we demonstrated that ischemia/ reperfusion of pancreas induced inflammation of pancreas and the elevation of airways reactivity which could be related to the I/R of pancreas-induced increase of inflammation white cell, tumor necrotic factor, hydroxyl radicals, and nitrosative stress. After administration of curcumin (antioxidant), all these responses are attenuated. Since post I/R of pancreas induced increase of amylase, lipase, protease in the blood, it proved indirectly that pancreatic juice exudates into the blood (p < 0.01), so that intrapulmonary inflammation and lung edema.
In this study we set up the model by aerosolized of pancreatic juice and infused the aerosol directly into airway. We demonstrated that aerosolized of pancreatic juice into airway could induce obstructive and restrictive ventilatory insufficiency which are related to the juice-induced lung inflammation by which increase of blood concentration of white cells in pulmonary capillary. Decrease of RBC counts and increase of the concentrations of hemoglobin, hematocrit, mean corporal volume, and the mean weight of hemoglobin. Besides, platelet concentration also decreased. These changes induced disseminated coagulation in the whole body. The increase of the concentrations of lactate dehydrogenase in the blood and in the lavage proved that juice-induced cells damage in the…
Advisors/Committee Members: Shen, Chien-yeh (chair), Wang, David (chair), Wang, Cheng-Kuang (chair), Lee, Ying-Chung (chair), Chao, David (committee member).
Subjects/Keywords: nitrosative stress; Ischemia/ reperfusion (I/R); pulmonary capillary bronchial alveolar lavage (PCBAL); lung injury; curcumin; bronchoalveolar lavage fluid
to Zotero / EndNote / Reference
APA (6th Edition):
Chen, K. (2015). The protective effects of curcumin on the pancreatic juice induced lung injury. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802115-140256
Chicago Manual of Style (16th Edition):
Chen, Kuan-hao. “The protective effects of curcumin on the pancreatic juice induced lung injury.” 2015. Doctoral Dissertation, NSYSU. Accessed November 26, 2020.
MLA Handbook (7th Edition):
Chen, Kuan-hao. “The protective effects of curcumin on the pancreatic juice induced lung injury.” 2015. Web. 26 Nov 2020.
Chen K. The protective effects of curcumin on the pancreatic juice induced lung injury. [Internet] [Doctoral dissertation]. NSYSU; 2015. [cited 2020 Nov 26].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802115-140256.
Council of Science Editors:
Chen K. The protective effects of curcumin on the pancreatic juice induced lung injury. [Doctoral Dissertation]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802115-140256