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You searched for +publisher:"NSYSU" +contributor:("Jin-Ching Lee"). Showing records 1 – 3 of 3 total matches.

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NSYSU

1. Lee, Meng-Yao. Expression of Fractalkine chemokine domain by baculovirus expression system.

Degree: Master, Biological Sciences, 2013, NSYSU

Baculovirus expression system has often been utilized to express recombinant proteins.The advantages of this system include high efficiency, ability to express proteins in manytypes of cells, and production of proteins with correct conformation and posttranslational modifications. Fractalkine is a membrane protein mainly exerting its effects through binding of extracellular chemokine domain to the CX3CR1 receptor. It regulates proliferation, differentiation, and apoptosis of cells. It also plays an important role in cardiovascular diseases. The goal of this thesis is to construct a baculovirus vector system to express fractalkine chemokine domain for further functional studies. The vector system is engineered to contain a secretion signal peptide sequence and two affinity tags, polyhistidine tag for purification and myc tag for immunoblotting. Using this system, it was found that fractalkine chemokine domain is able to express in HEK293 and HeLa cells, whereas THP-1 monocyte and Jurkat T cells are without effect. The expressed recombinant protein could be detected at 60 h after viral infection and the majority appeared to be produced after 72 h. In addition, the fractalkine chemokine domain produced in HEK293 cells was able to purified from the medium through polyhistidine tag pulldown. These results indicate that a highly efficient protein expression system with advantages of easy detection and purification has been established and this system is a valuable tool in structural and functional studies of proteins. Advisors/Committee Members: Jong-Kang Liu (committee member), Jin-Ching Lee (chair), Song-Tay Lee (chair), Hung Wu (committee member).

Subjects/Keywords: Fractalkine; His tag; baculovirus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lee, M. (2013). Expression of Fractalkine chemokine domain by baculovirus expression system. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Lee, Meng-Yao. “Expression of Fractalkine chemokine domain by baculovirus expression system.” 2013. Thesis, NSYSU. Accessed December 03, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Lee, Meng-Yao. “Expression of Fractalkine chemokine domain by baculovirus expression system.” 2013. Web. 03 Dec 2020.

Vancouver:

Lee M. Expression of Fractalkine chemokine domain by baculovirus expression system. [Internet] [Thesis]. NSYSU; 2013. [cited 2020 Dec 03]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee M. Expression of Fractalkine chemokine domain by baculovirus expression system. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715113-214714

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


NSYSU

2. Liao, Jing-fong. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.

Degree: Master, Institute of Medical Science and Technology, 2016, NSYSU

This study developed a flexible and rapidly-dissolvable microneedle (MN) patch for transdermal delivery and stability enhancement of DNA vaccine. The MN patch is made by polyvinyl alcohol (PVA) with the properties of rapid dissolution, biocompatibility and film-forming. Besides, the vaccine encapsulated in MN would be rapidly released after insertion of the MN patch to the skin. We were able to encapsulate 22.4 μg of PCV2 plasmid DNA vaccine in MNs per patch with a loading rate of 87.8 wt % when casting the PCV2 plasmid DNA vaccination the PDMS mode surface. We also done the stability study, the activity of vaccine encapsulated in MNs can be maintained exceed 111 days without any damage at 37 â of storage. The ex vivo study demonstrated that the MNs have excellent mechanical strength to puncture the stratum corneum and needles can be dissolved within 10 minutes to release the vaccine in epidermis then the hole can be quickly healed within 40 minutes to avoid the infection risk. The Animals studies showed that the induced antibody titers via MN patch administration were about 10-time higher than intramuscular (IM) administration after 3 weeks of vaccination. The results confirmed that the MNs can induce high antibody concentration in body to induce the strongest immune responses after immunization. Advisors/Committee Members: Hung-Wei Yang (committee member), Jin-Ching Lee (chair), Chen-Chi M. Ma (chair).

Subjects/Keywords: Transcutaneous immunization; Dissolving microneedle; DNA vaccine; Flexible patches; Polyvinyl alcohol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Liao, J. (2016). Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Liao, Jing-fong. “Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.” 2016. Thesis, NSYSU. Accessed December 03, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Liao, Jing-fong. “Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.” 2016. Web. 03 Dec 2020.

Vancouver:

Liao J. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. [Internet] [Thesis]. NSYSU; 2016. [cited 2020 Dec 03]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liao J. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


NSYSU

3. Lin, Chun-kuang. Development of antiviral drugs from marine natural products and investigation of drug target against virus.

Degree: PhD, Doctoral Degree Program in Marine Biotechnology, 2018, NSYSU

Hepatitis C virus (HCV) infection causes chronic inflammation of liver, leading to the development of cirrhosis and hepatocellular carcinoma (HCC). Infection of dengue virus (DENV) caused diseases ranging from acute self-limiting febrile illness to life-threatening dengue hemorrhagic fever and dengue shock syndrome. The purposes of present dissertation are to discover the anti-viral agents from marine natural products and to investigate the impact of cellular factors on DENV replication. For finding the potential antivirals, we found that betulinic acid (BA) and acteoside (AM-4) extracted from Avicennia marina could reduce HCV replication. The mechanism study demonstrated that BA reduced HCV replication through decreasing the NF-κB- and ERK1/2-mediated cyclooxygenase-2 (COX-2) expression. The AM-4 suppressed HCV infection by blocking viral entry into cells and cell-to-cell spread of HCV. In addition, we identified that lobohedleolide extracted from soft coral exhibited anti-HCV activity by suppression of HCV-induced COX-2 expression. Using various COX-2 promoter deletion constructs linked to luciferase reporter gene, we first identified CCAAT/enhancer-binding protein (C/EBP) as a key transcription factor for the down-regulation of COX-2 by lobohedleolide, and then demonstrated that the HCV-induced C/EBP expression could be suppressed by lobohedleolide through inhibiting the phosphorylation of JNK and c-Jun. Notably, combination treatment of BA, AM-4 and lobohedleolide with several clinically used HCV drugs synergistically inhibited HCV RNA replication, indicating that these three natural products exhibited a high biomedical potential to be used as a supplementary agent for control of HCV infection. Besides, BA and lobohedleolide also exhibited anti-DENV activity. For finding the therapeutic targets from cellular gene against DENV, we observed an increased level of COX-2 in patients with dengue fever compared with healthy individuals. Then, an elevated level of COX-2 expression was also observed in DENV-infected ICR suckling mice. COX-2 gene silencing and catalytic inhibition sufficiently suppressed DENV-2 replication. Using ICR suckling mouse model, we identified that the COX-2 inhibitor NS398 protected mice from succumbing to life-threatening DENV-2 infection, revealing targeting COX-2 is a promising strategy to control DENV infection. In addition, we found that the expression of prostasin, a serine protease, is lower in patients with dengue fever than in healthy individuals. Exogenous expression of prostasin could protect ICR suckling mice from life-threatening DENV-2 infection and reduce DENV-2 propagation in Huh-7 cells. We further revealed that prostasin reduced DENV replication through proteolytic cleavage of epithelial growth factor receptor (EGFR). The activity of proteolytic cleavage of prostasin is dependent on the expression of matriptase and hepatocyte growth factor activator inhibitor type 2 (HAI-2). Collectively, COX-2 and prostasin exhibited highly potential to serve as therapeutic targets against… Advisors/Committee Members: Jyh-Horng Sheu (chair), Jin-Ching Lee (chair), Shih-Hsiung Wu (chair), Chih-Chuang Liaw (committee member), Yen-Hsu Chen (chair).

Subjects/Keywords: DENV; COX-2; marine natural product; EGFR; prostasin; HCV

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Lin, C. (2018). Development of antiviral drugs from marine natural products and investigation of drug target against virus. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352

Chicago Manual of Style (16th Edition):

Lin, Chun-kuang. “Development of antiviral drugs from marine natural products and investigation of drug target against virus.” 2018. Doctoral Dissertation, NSYSU. Accessed December 03, 2020. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352.

MLA Handbook (7th Edition):

Lin, Chun-kuang. “Development of antiviral drugs from marine natural products and investigation of drug target against virus.” 2018. Web. 03 Dec 2020.

Vancouver:

Lin C. Development of antiviral drugs from marine natural products and investigation of drug target against virus. [Internet] [Doctoral dissertation]. NSYSU; 2018. [cited 2020 Dec 03]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352.

Council of Science Editors:

Lin C. Development of antiviral drugs from marine natural products and investigation of drug target against virus. [Doctoral Dissertation]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0019118-192352

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