+publisher:"NSYSU" +contributor:("Hung-Wei Yang")

NSYSU

1. Pan, Lunh-hsuan. Microfluidic-based optical biochip system for the detection of VEGF, PSA, and CTCs in early prostate cancer diagnosis.

Degree: Master, Institute of Medical Science and Technology, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715116-174442

► Prostate Cancer (PCa) is the third most common cancer in men in Europe, North America, and some parts of Africa. The PCa could be diagnosed… (more)

▼ Prostate Cancer (PCa) is the third most common cancer in men in Europe, North America, and some parts of Africa. The PCa could be diagnosed by digital rectal inspection, computed tomography (CT) scan, magnetic resonance imaging (MRI), and biomarkers assay in clinical examination. To date, the prostate-specific antigen (PSA) is the best serum marker available for clinical detection of PCa, and serum values above 4.0 ng/mL are considered abnormal. Furthermore, the vascular endothelial growth factor (VEGF) also plays an important role in the progressed cancers. The VEGF is also overexpressed in patients who suffered PCa and the serum values above 100 pg/mL are considered abnormal. Most assays for PSA and VEGF detection are based on conventional immunodetection methods, including the enzyme-linked immunosorbent assay (ELISA) and electrochemical immunoassays. ELISA involves sequential binding of two antibodies to each specific antigen epitope and repeated washing steps to measure the concentration of target antigen; this makes it prone to error, relatively time-consuming, and expensive. In addition, cancer progression is comprised of both primary tumor growth and secondary metastasis, tumor cells are shed into the circulation system in few numbers since early stages of cancer formation, tumor cells can survive and through microenvironment interactions in the pathogenesis of cancer metastasis proceed to metastasis. Thus, there is an urgent need to develop the simultaneous multi-markers and circulating tumor cells (CTCs) assay for the early and accurate detection of PCa. In this study, we developed different biochip multiplexed microfluidic-based optical biosensor, which can be used to rapidly and accurately detect the concentrations of PSA (0.5-50 ng/mL), VEGF (0.5-50 ng/mL) and CTCs (300 cell/mL) simultaneously. In this design, gold nanorods were used to label with anti-PSA, anti-VEGF and ssDNA as bio-recognizers to capture PSA, VEGF, and PC3 cells for sensitive optical bio-sensing. Our data showed that the biochips can effectively detect the concentrations of PSA, VEGF, and CTCs in the human blood for early diagnosis of PCa with high sensitivity, specificity, accuracy, low-cost, and faster rate. Advisors/Committee Members: Chen-Chi M. Ma (chair), Wei%20Yang%22%29&pagesize-30">Hung-Wei Yang (committee member), See-Tong Pang (chair).

Subjects/Keywords: vascular endothelial growth factor; prostate-specific antigen; circulating tumor cells; optical biosensor; prostate cancer; microfluidic device

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pan, L. (2016). Microfluidic-based optical biochip system for the detection of VEGF, PSA, and CTCs in early prostate cancer diagnosis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715116-174442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pan, Lunh-hsuan. “Microfluidic-based optical biochip system for the detection of VEGF, PSA, and CTCs in early prostate cancer diagnosis.” 2016. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715116-174442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pan, Lunh-hsuan. “Microfluidic-based optical biochip system for the detection of VEGF, PSA, and CTCs in early prostate cancer diagnosis.” 2016. Web. 15 Jan 2021.

Vancouver:

Pan L. Microfluidic-based optical biochip system for the detection of VEGF, PSA, and CTCs in early prostate cancer diagnosis. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715116-174442.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pan L. Microfluidic-based optical biochip system for the detection of VEGF, PSA, and CTCs in early prostate cancer diagnosis. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715116-174442

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

2. Wu, Yun-Tse. Template-assisted Synthesis of Metal Nanoclusters and their Application as a Fluorescent Sensor.

Degree: Master, Chemistry, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605116-152602

► This study utilized templates to support the synthesis of gold nanoclusters. Having the advantages of high performance on fluorescence, longer fluorescence lifetime, and good biocompatibility,… (more)

▼ This study utilized templates to support the synthesis of gold nanoclusters. Having the advantages of high performance on fluorescence, longer fluorescence lifetime, and good biocompatibility, it is suitable for us to use this kind of materials to design biosensors. After templates modified, we could get a better detection results by a mothods of ratiometric fluorescence sensing system, and we apply this probe for biochemistry detection and cellular imaging on the first study. Besides, addition silver nitrate to gold nanoclusters gave the generation of bimetallic alloy nanoclusters. With the properties entirely changed, we designed a brand new sensing system for nucleotides detection on the second study. (A) Gold Nanoclusters-Based Fluorescent Probe for Simultaneous Sensing of pH and Temperature and Its Application to Cellular Imaging and Logic Gates This study describes the synthesis of a dual emission probe, FITC/BSA-AuNCs, for the ï¬uorescent ratiometric sensing of temperature and pH change. Green-emitting ï¬uorescein-5-isothiocyanate (FITC) was labeled on bovine serum albumin (BSA) by conjugating the isothiocyanate groups to the amino groups. This FITC-capped BSA acted as a template for the synthesis of red-emitting gold nanoclusters (AuNCs) under alkaline conditions. As a result, FITC/BSA-AuNCs could emit dual ï¬uorescence at 525 and 670 nm at single wavelength excitation, which are sensitive to the pH and temperature change, respectively. The temperature-dependent fluorescence of the AuNCs enabled FITC/BSA-AuNCs to ratiometrically detect the temperature change with the resolution better than 1.5 â as the FITC was used as an internal standard. Meanwhile, the pH-induced fluorescence change of FITC enabled to ratiometrically probe the pH change with the resolution of 0.5-pH unit as the fluorescence of the AuNCs remained almost constant under identical conditions. Based on this concept, this study firstly developed AuNC-based probe for simultaneous detection of pH and temperature change with a linear range from pH 6.0â8.0 and from 21 ââ41 â, respectively. Since trypsin can digest BSA to peptide fragments, a dramatically decreased in the fluorescence intensity at 670 nm of FITC/BSA-AuNCs was observed in the presence of increasing the logarithmic concentration of trypsin. This finding enable us to ratiometrically detect trypsin with a detection limit of 131 pg/mL and a linear range of 10-8 to 10-4 g/mL. The successful quantification of trypsin in human urine demonstrated that FITC/BSA-AuNCs is capable of sensing trypsin in complex matrix. Three-input AND logic gates were then designed by using temperature, pH, and trypsin as inputs. Finally, the practicality of utilizing FITC/BSA-AuNCs to determine temperature and pH changes in HeLa cells is also achieved. (B) Synthesis of DNA-templated bimetallic nanoclusters for fluorescent sensing of target nucleic acid by shift in maximum emission wavelength Under UV light irradiation and heating, we develop a simple, nontoxic, and facile way to synthesize gold nanoclusters and… Advisors/Committee Members: Po-Chiao Lin (chair), Wei-Lung Tseng (committee member), Wei%20Yang%22%29&pagesize-30">Hung-Wei Yang (chair).

Subjects/Keywords: bimetallic nanoclusters; Temperature; pH value; Intracellular image; Nucleotide; Gold nanoclusters; Ratiometric sensor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, Y. (2016). Template-assisted Synthesis of Metal Nanoclusters and their Application as a Fluorescent Sensor. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605116-152602

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Yun-Tse. “Template-assisted Synthesis of Metal Nanoclusters and their Application as a Fluorescent Sensor.” 2016. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605116-152602.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Yun-Tse. “Template-assisted Synthesis of Metal Nanoclusters and their Application as a Fluorescent Sensor.” 2016. Web. 15 Jan 2021.

Vancouver:

Wu Y. Template-assisted Synthesis of Metal Nanoclusters and their Application as a Fluorescent Sensor. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605116-152602.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu Y. Template-assisted Synthesis of Metal Nanoclusters and their Application as a Fluorescent Sensor. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605116-152602

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

3. Lin, Feng-Wei. BSA nanoparticles-incorporated thermosensitive hydrogel for preventing post-operative recurrence of brain tumor.

Degree: Master, Institute of Medical Science and Technology, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0612116-182205

► Malignant brain tumor is a serious disease. Patients treated using surgical resection, radiotherapy and chemotherapy survive, on average, for 6-24 months post-treatment. To date, surgery… (more)

Subjects/Keywords: Brain tumor; BSA nanoparticles; Drugs release; MRI; Temperature-sensitive hydrogel

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, F. (2016). BSA nanoparticles-incorporated thermosensitive hydrogel for preventing post-operative recurrence of brain tumor. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0612116-182205

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Feng-Wei. “BSA nanoparticles-incorporated thermosensitive hydrogel for preventing post-operative recurrence of brain tumor.” 2016. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0612116-182205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Feng-Wei. “BSA nanoparticles-incorporated thermosensitive hydrogel for preventing post-operative recurrence of brain tumor.” 2016. Web. 15 Jan 2021.

Vancouver:

Lin F. BSA nanoparticles-incorporated thermosensitive hydrogel for preventing post-operative recurrence of brain tumor. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0612116-182205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin F. BSA nanoparticles-incorporated thermosensitive hydrogel for preventing post-operative recurrence of brain tumor. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0612116-182205

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

4. Liao, Jing-fong. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.

Degree: Master, Institute of Medical Science and Technology, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

► This study developed a flexible and rapidly-dissolvable microneedle (MN) patch for transdermal delivery and stability enhancement of DNA vaccine. The MN patch is made by… (more)

Subjects/Keywords: Transcutaneous immunization; Dissolving microneedle; DNA vaccine; Flexible patches; Polyvinyl alcohol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liao, J. (2016). Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liao, Jing-fong. “Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.” 2016. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liao, Jing-fong. “Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine.” 2016. Web. 15 Jan 2021.

Vancouver:

Liao J. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liao J. Flexible and Rapidly-Dissolvable Microneedle Patches for Transdermal Delivery and Stability Enhancement of DNA Vaccine. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0529116-155910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

5. Yang, Ya-Chun. (1) 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay; (2) Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein.

Degree: Master, Chemistry, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0604116-162031

► I. 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay Based on H2O2-induced growth strategy, gold nanoparticles (AuNPs) is often… (more)

▼ I. 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay Based on H2O2-induced growth strategy, gold nanoparticles (AuNPs) is often implemented for hydrogen peroxide (H2O2) sensing and immunoassay. This study find out that 1,4-benenediboronic acid (BDBA) was effective to induce the aggregation of AuNPs. The driving force was relied on the complexation of boronic acid and citrate-capped reagent on AuNPs. Besides, H2O2 is able to mediate the boronate hydrolysis into phenol which wouldnât trigger the aggregation of AuNPs. AuNPs aggregation would be inhibit depends on the H2O2 concentration. As the concentration of H2O2 increase, the relative degree of AuNPs aggregation would decrease. BDBA-induced aggregation of citrate-capped AuNPs enabled H2O2 sensing with detection limit of 300 nM and could apply to glucose oxidase-glucose system for a glucose colorimetric probe. Combine HÂ¬2O2 sensing strategy, glucose oxidase-glucose system and biotin-avidin-mediated immunoassay, the H2O2âBDBAâAuNPs probe could well accommodate to naked-eye readout immunoassay. The lowest detectable concentration of rabbit IgG and prostate-specific-antigen were down to 0.2 ng/ml and 2 ng/ml, respectively. More importantly, the developing plasmonic immunoassay allowed the PSA naked-eye detection in 0-10 ng/ml with 2 ng/ml intervals of human serum samples. II. Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein Magnetite nanoparticles (Fe3O4 NPs) peroxidase-like activity could be inhibited by proteins with low selectivity. This study discloses the adenosine phosphates (APs)-enhancement of Fe3O4 NPs peroxidase-like activity with improved selectivity for sensing human serum albumin (HSA). Based on metal ion chelation, APs with phosphate structure could be functionalized on Fe3O4 NPs surface without further modification. The adenosine structure of APs with nitrogen functional group on Fe3O4 NPs surface is abled to promote the catalysis reaction between H2O2 and Fe3O4 NPs with higher reactive oxygen species (ROS) formation. The phenomena represent a remarkable enhancement of Fe3O4 NPs peroxidase-like activity which could be realized by fluorescent substrate, amplex ultrared. Among three different phosphate groups of APs, adenosine monophosphate (AMP) with higher adsorb proportion shows the largest peroxidase-like acitivity enhancement. Compare to the system directly used Fe3O4 NPs for HSA sensing, the AMP-assisted Fe3O4 NPs peroxidase-like activity shows a broader linear range (0.2-60 Î¼M) and higher selectivity. Once HSA adsorb on Fe3O4 NPs surface, HSA with an isoelectric point of 5.3 changes Fe3O4 NPs surface into negative charge under pH 7 condition. This properties could highly improve HSA selectivity through the electrostatic repulsion between AMP and HSA-adsorbed Fe3O4. Rely on AMP functional structure, AMP-enhanced Fe3O4 NPs peroxidase-like activity with high sensitivity and… Advisors/Committee Members: Wei-Lung Tseng (committee member), Wei%20Yang%22%29&pagesize-30">Hung-Wei Yang (chair), Po-chiao Lin (chair).

Subjects/Keywords: magnetite nanoparticles; peroxidase-like activity; adenosine phosphate analogs; urine protein; prostate-specific-antigen; immunoassay; 1,4-benzene diboronic acid; naked-eye readout; gold nanoparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yang, Y. (2017). (1) 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay; (2) Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0604116-162031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Ya-Chun. “(1) 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay; (2) Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein.” 2017. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0604116-162031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Ya-Chun. “(1) 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay; (2) Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein.” 2017. Web. 15 Jan 2021.

Vancouver:

Yang Y. (1) 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay; (2) Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0604116-162031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang Y. (1) 1,4-Benzenediboronic-Acid-Induced Aggregation of Gold Nanoparticles: Application to Hydrogen Peroxide Detection and Naked-eye Readout Immunoassay; (2) Adenosine Monophosphate-Enhanced Peroxidase-like Activity of Magnetite Nanoparticles with Selective Inhibition for Human Urine Protein. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0604116-162031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

6. Lin, Tzu-Yang. An Aptasensor for Highly Selective Dopamine Detection Based on Colorimetry and Fluorescence Quenching System.

Degree: Master, Institute of Medical Science and Technology, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0625117-133640

► Motivation:ã Dopamine (DA), one of the most prominent catecholamine neurotransmitter existing humanâs circulation system and cerebrospinal fluid, plays a crucial role in several neurological disorders.… (more)

▼ Motivation:ã Dopamine (DA), one of the most prominent catecholamine neurotransmitter existing humanâs circulation system and cerebrospinal fluid, plays a crucial role in several neurological disorders. According to research, the normal content of DA in healthy people was in the range of 1.3 - 2.6 Î¼M (i.e. 0.26 Î¼g/mL - 0.54 Î¼g/mL), which indicated that when the DA concentration was abnormal in human plasma, it could lead to certain neurological disease such as schizophrenia, or Parkinsonâs disease (PD). Purpose: In clinical diagnosis, positron emission tomography (PET) or single-photon emission computed tomography (SPECT) are harnessed to detect changes in striatal DA level. On the other hand, ELISA Kit and electrochemical detection (ECD) for HPLC are extremely selective and sensitive detection techniques, however, they are actually time-consuming, costly and complicated in clinical detection. Thus, we aimed to design a fabrication for a biochip detecting for DA would be so facile and cheap that have an inclination to be employed for the clinical detection in the near future. Methods: We immobilized the DBA onto amine groups-modified chips by covalent binding with GA linker. The amine groups modified chips were fabricated with branched polyethylenimine (bPEI). After that, we employed two sensing system for detection as soon as DA was captured by DBA. In the first system, was adopted based on the oxidation reaction of DA induced by sodium hydroxide (NaOH), causing the color of supernatant altering from transparent to brownish accompanied by a specific absorbance spectrum. The second system the BSA-Au nanoclusters (BSA-Au NCs) can be quenched with the increasing concentration of DA based on PET mechanism. Results: In this study, the biochip prepared was able to detect DA with linear range from 1 ng/mL, to 10 mg/mL and a limit of detection (LOD) as low as 0.1 ng/mL based on fluorescence quenching system, which was in the detecting range of DA in human serum concentration. Also the results of interference test (e.g. ascorbic acid, glucose, alanine, uric acid, etc.) showed that our designed system was selective and specific. With those advantages, the biochip for DA detection certainly has potentiality to be applied in the clinical detection in the near future. Advisors/Committee Members: Chun-Jen Huang. (chair), Min-Hsien Wu. (chair), Wei%20Yang.%22%29&pagesize-30">Hung-Wei Yang. (committee member).

Subjects/Keywords: Au nanoclusters; Aptasensor; Colorimetry System; Fluorescence Quenching System; Dopamine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, T. (2017). An Aptasensor for Highly Selective Dopamine Detection Based on Colorimetry and Fluorescence Quenching System. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0625117-133640

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Tzu-Yang. “An Aptasensor for Highly Selective Dopamine Detection Based on Colorimetry and Fluorescence Quenching System.” 2017. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0625117-133640.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Tzu-Yang. “An Aptasensor for Highly Selective Dopamine Detection Based on Colorimetry and Fluorescence Quenching System.” 2017. Web. 15 Jan 2021.

Vancouver:

Lin T. An Aptasensor for Highly Selective Dopamine Detection Based on Colorimetry and Fluorescence Quenching System. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0625117-133640.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin T. An Aptasensor for Highly Selective Dopamine Detection Based on Colorimetry and Fluorescence Quenching System. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0625117-133640

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

7. Pang , Hao-Han. Using Multifunctional QÎ² Virus-Like Particles for RNAi and Reducing Temozolomide Resistance in Brain Tumor.

Degree: Master, Institute of Medical Science and Technology, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-132050

► Introducion Brain tumour is one of the most lethal cancers due to difficulties of delivering therapeutic agents across blood-brain barrier (BBB) and serious side effect.… (more)

Subjects/Keywords: drug resistance; RNAi; VLPs; Virus-like particles; Brain Tumor; Temozolomide

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APA (6^th Edition):

Pang , H. (2017). Using Multifunctional QÎ² Virus-Like Particles for RNAi and Reducing Temozolomide Resistance in Brain Tumor. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-132050

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pang , Hao-Han. “Using Multifunctional QÎ² Virus-Like Particles for RNAi and Reducing Temozolomide Resistance in Brain Tumor.” 2017. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-132050.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pang , Hao-Han. “Using Multifunctional QÎ² Virus-Like Particles for RNAi and Reducing Temozolomide Resistance in Brain Tumor.” 2017. Web. 15 Jan 2021.

Vancouver:

Pang H. Using Multifunctional QÎ² Virus-Like Particles for RNAi and Reducing Temozolomide Resistance in Brain Tumor. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-132050.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pang H. Using Multifunctional QÎ² Virus-Like Particles for RNAi and Reducing Temozolomide Resistance in Brain Tumor. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-132050

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

8. Li, Nan-si. Magnetic Liquid for Rapid Detection of CA19-9 and MUC1 in Pancreatic Cancer.

Degree: Master, Institute of Medical Science and Technology, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728117-213822

► Motivation: Pancreatic cancer (PC) represents a fatal tumor with a high mortality and poor prognosis. These outcomes can be attributed a lack of early diagnosis… (more)

▼ Motivation: Pancreatic cancer (PC) represents a fatal tumor with a high mortality and poor prognosis. These outcomes can be attributed a lack of early diagnosis and inability to detect pre-cancerous pancreatic intraductal neoplastic (PanIN) lesions. Currently, no clinically reliable biosensing systems for early detection at an early stage of pancreatic cancer are available. Purpose: Thus, itâs urgent to develop a biosensing system which can fast and accurately diagnose PC. So far, the carbohydrate antigen (CA19-9) approved by the US Food and Drug Administration (FDA) is the most common biomarker used for several decades. However, it has limited value in diagnosis of early stages of PC. Mucin1 (MUC1) , a cell surface associated protein, is an emerging biomarker associated with early development of PC. Methods: Therefore, we develop a magnetic biosensing liquid modified with CA19-9 antibody and MUC1 antibody for the rapid and specific capturing of these two cancer antigens. Besides, we immobilize the anti-CA19-9 and anti-MUC1 onto the gold nanorods, which can catalyze the reduction of the methyl orange (Absorption at 508 nm) with the sodium bromide. Results: In this design, after the cancer antigens bind to the magnetic biosensing liquid, we add the liquid into the plate coated with antibodies to become sandwich immunosorbent conjugates. Finally, after washing the plate, we add the solution of methyl orange and sodium bromide into it. Therefore, with increasing concentrations of cancer antigens, the more magnetic biosensing particles will bind onto the bottom of plate. And the efficiency of the catalytic degradation of methyl orange in a certain period of time will increase. In the end, we can use the downtrend of the absorption intensity under different concentrations of cancer antigens to analyze the linear detection range of our biosensing system. Advisors/Committee Members: Chun-Jen Huang (chair), Wei%20Yang%22%29&pagesize-30">Hung-Wei Yang (committee member), Min-Hsien Wu (chair).

Subjects/Keywords: Pancreatic cancer; Magnetic nanoparticles; Methyl orange; Optical biosensor; Gold nanorods

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, N. (2017). Magnetic Liquid for Rapid Detection of CA19-9 and MUC1 in Pancreatic Cancer. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728117-213822

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Nan-si. “Magnetic Liquid for Rapid Detection of CA19-9 and MUC1 in Pancreatic Cancer.” 2017. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728117-213822.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Nan-si. “Magnetic Liquid for Rapid Detection of CA19-9 and MUC1 in Pancreatic Cancer.” 2017. Web. 15 Jan 2021.

Vancouver:

Li N. Magnetic Liquid for Rapid Detection of CA19-9 and MUC1 in Pancreatic Cancer. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728117-213822.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li N. Magnetic Liquid for Rapid Detection of CA19-9 and MUC1 in Pancreatic Cancer. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728117-213822

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

9. Wang , Hong-Jyun. Rapid in situ gelation by blue light-irradiation for combination therapy in brain tumor.

Degree: Master, Institute of Medical Science and Technology, 2018, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728118-203208

► About 40,000 people are diagnosed with primary brain tumors in the United States each year, an estimated 15,000 have glioblastoma multiforme (GBM), still associated with… (more)

Subjects/Keywords: Hydrogel; Brain tumor; Drug delivery; Combination therapy; Photothermal therapy; Gelatin methylacrylate

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang , H. (2018). Rapid in situ gelation by blue light-irradiation for combination therapy in brain tumor. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728118-203208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang , Hong-Jyun. “Rapid in situ gelation by blue light-irradiation for combination therapy in brain tumor.” 2018. Thesis, NSYSU. Accessed January 15, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728118-203208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang , Hong-Jyun. “Rapid in situ gelation by blue light-irradiation for combination therapy in brain tumor.” 2018. Web. 15 Jan 2021.

Vancouver:

Wang H. Rapid in situ gelation by blue light-irradiation for combination therapy in brain tumor. [Internet] [Thesis]. NSYSU; 2018. [cited 2021 Jan 15]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728118-203208.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang H. Rapid in situ gelation by blue light-irradiation for combination therapy in brain tumor. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728118-203208

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations