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NSYSU
1.
Shen, Che-yu.
Molecular cloning of mitogen-activated protein kinase cDNA and study of ethylene signaling in senescent sweet potato leaves.
Degree: Master, Biological Sciences, 2011, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0408111-103618 
► Ethylene is a plant growth regulator and plays a key role in leaf senescence. Its signaling, however, remains mostly unclear in sweet potato. Ethephon, an…
(more)
▼ Ethylene is a plant growth regulator and plays a key role in leaf senescence. Its signaling, however, remains mostly unclear in sweet potato. Ethephon, an ethylene releasing compound, induced sweet potato detached leaf senescence and associated gene expression, and the effects were repressed by mitogen-activated protein kinase (MAPK) kinase inhibitor PD98059. These data suggest that MAPK cascade is likely involved in ethylene signaling leading to leaf senescence and associated gene expression. With gene-specific primers and RT-PCR methods, a full-length cDNA, SPMAPK, was isolated from ethephon-treated sweet potato leaves. SPMAPK contained 1098 nucleotides (365 amino acids) in the open reading frame. Sweet potato SPMAPK also exhibited high amino acid sequence identities (ca. 79.8% to 83.4%) with plant MAPKs, and was most close to Arabidopsis MPK3 and MPK6 in phylogenetic tree analysis. RT-PCR analysis showed that SPMAPK gene expression was detected in roots, stems, and leaves. The mature and partial yellowing leaves expressed higher amount. SPMAPK gene expression was also inducible and significantly enhanced by ethephon. Results from studies with inhibitors or effectors showed that ethephon treatment resulted in acceleration of leaf senescence in detached sweet potato leaves, promotion of leaf chlorophyll content reduction and decrease of photochemical Fv/Fm, and induction of associated gene expression. These ethephon-mediated effects were all delayed or repressed by pretreatment with ethylene receptor inhibitor 1-methylcyclopropene (1-MCP), MAPK kinase inhibitor PD98059, NADPH oxidase inhibitor diphenyleneiodonium (DPI), antioxidant reduced glutathione, calcium ion chelator EGTA, and de novo protein synthesis inhibitor cycloheximide, respectively. Based on these results we conclude that an ethylene-inducible mitogen-activated protein kinase SPMAPK was isolated from sweet potato leaves, and expressed higher amount in mature and partial yellowing leaves. Ethephon-induced sweet potato SPMAPK expression was significantly repressed by 1-MCP, PD98059, DPI, reduced glutathione, EGTA and cycloheximide. These data also suggest that the possible signal components in ethephon-mediated leaf senescence and associated gene expression in sweet potato leaves likely include ethylene receptor, MAPK cascade, elevated H2O2 , external calcium influx, and de novo synthesized proteins. A possible ethylene signaling model leading to sweet potato leaf senescence and associated gene expression was also proposed.
Advisors/Committee Members: nen%20Chen%22%29&pagesize-30">
Ching-
nen Chen (chair),
Hsien-jung Chen (committee member),
Guan-jhong Huang (chair).
Subjects/Keywords: sweet potato; ethylene; leaf senescence; mitogen-activated protein kinase; PD98059
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APA (6th Edition):
Shen, C. (2011). Molecular cloning of mitogen-activated protein kinase cDNA and study of ethylene signaling in senescent sweet potato leaves. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0408111-103618
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shen, Che-yu. “Molecular cloning of mitogen-activated protein kinase cDNA and study of ethylene signaling in senescent sweet potato leaves.” 2011. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0408111-103618.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shen, Che-yu. “Molecular cloning of mitogen-activated protein kinase cDNA and study of ethylene signaling in senescent sweet potato leaves.” 2011. Web. 18 Apr 2021.
Vancouver:
Shen C. Molecular cloning of mitogen-activated protein kinase cDNA and study of ethylene signaling in senescent sweet potato leaves. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0408111-103618.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shen C. Molecular cloning of mitogen-activated protein kinase cDNA and study of ethylene signaling in senescent sweet potato leaves. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0408111-103618
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
2.
Huang, Yu-hsuan.
Cloning and characterization of an ethephon-inducible aspartic protease SPAP and its association with promotion of leaf senescence in sweet potato.
Degree: Master, Biological Sciences, 2013, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0608113-091307
► A full-length cDNA was isolated from sweet potato senescent leaves with PCR-selective subtractive hybridization and RACE PCR. Blast analysis showed that it exhibited high nucleotide…
(more)
▼ A full-length cDNA was isolated from sweet potato senescent leaves with PCR-selective subtractive hybridization and RACE PCR. Blast analysis showed that it exhibited high nucleotide sequence identity (ca. 72-73%) with plant aspartic proteases including potato and tomato, and was named as sweet potato aspartic protease SPAP. Its open reading frame contained 1515 nucleotides (504 amino acids), and encoded a putative aspartic protease with a catalytic site composed of DTG and DSG and a plant specific insert constituted of ca. 100 amino acids in length. Gene expression of sweet potato aspartic protease SPAP increased gradually from L1 immature leaves to L3 mature leaves, and then continuously decreased from intermediate L4 to late L5 leaf senescent stages. In addition, L3 mature leaves also exhibited higher expression level than that of storage roots, roots and stems. Ethephon treatment also caused temporary enhancement of sweet potato aspartic protease SPAP dose-dependently detected by RT-PCR and Western blot hybridization. Ethephon treatment promoted sweet potato leaf senescence and H2O2 elevation. Exogenous purified SPAP fusion protein significantly accelerated the ethephon-mediated promotion of leaf senescence and H2O2 elevation. The SPAP fusion protein-mediated effects could be remarkably antagonized by anti-SPAP antibody, aspartic protease inhibitor pepstatin A, and 950C boil treatment of the purified SPAP fusion protein, which significantly alleviated the effects of exogenous SPAP fusion protein on the acceleration on ethephon-mediated promotion of leaf senescence and H2O2 elevation. In addition, no significant variation on the promotion of leaf senescence was observed between dark treatment and treatment with exogenous SPAP fusion protein alone. These results conclude for the first time that sweet potato aspartic protease SPAP is ethephon-inducible, and likely participates in ethephon-mediated promotion of leaf senescence and H2O2 elevation. Sweet potato aspartic protease SPAP may require interacting components for effective promotion of ethephon-mediated leaf senescence and H2O2 elevation in sweet potato.
Advisors/Committee Members: Guan-jhong Huang (chair), Hsien-Jung Chen (committee member), nen%20Chen%22%29&pagesize-30">
Ching-
nen Chen (chair).
Subjects/Keywords: Aspartic protease; Ethephon; Leaf senescence; Sweet potato
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APA (6th Edition):
Huang, Y. (2013). Cloning and characterization of an ethephon-inducible aspartic protease SPAP and its association with promotion of leaf senescence in sweet potato. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0608113-091307
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Huang, Yu-hsuan. “Cloning and characterization of an ethephon-inducible aspartic protease SPAP and its association with promotion of leaf senescence in sweet potato.” 2013. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0608113-091307.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Huang, Yu-hsuan. “Cloning and characterization of an ethephon-inducible aspartic protease SPAP and its association with promotion of leaf senescence in sweet potato.” 2013. Web. 18 Apr 2021.
Vancouver:
Huang Y. Cloning and characterization of an ethephon-inducible aspartic protease SPAP and its association with promotion of leaf senescence in sweet potato. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0608113-091307.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Huang Y. Cloning and characterization of an ethephon-inducible aspartic protease SPAP and its association with promotion of leaf senescence in sweet potato. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0608113-091307
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
3.
Lin, Jian-Bang.
The effect of oral administrated protein nanocapsule on inducing humoral immunity in grouper, Epinephelus coioides.
Degree: Master, Marine Biology, 2013, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0623113-170905
► If oral delivered antigen can replace invasive vaccine treatment in grouper, it will be able to cut down labor and time cost and also improve…
(more)
▼ If oral delivered antigen can replace invasive vaccine treatment in grouper, it will be able to cut down labor and time cost and also improve survival rate by reducing handling stress. The objectives of this experiment are (1) to encapsule model drug (bovine serum albumin, BSA) by poly-alkylcyanoacrylate to avoid entero-degradation, (2) to improve the intestinal absorption rate by nanocapsule less than 200 nm, (3) to enhance humoral immune response by oral administrated Bacillus subtilis as adjuvant. The results showed: (1) the diameter of BSA poly-alkylcyanoacrylate nanocapsule is 80.82 ± 8.365 nm which is suitable for oral administration; (2) oral administrated BSA nanocapsule can induce BSA antibody titer increasing over time after each additional booster over 30 days period (p < 0.05); (3) BSA antibody titer is positively correlated to the dose of BSA nanocapsule (p < 0.05); (4) adding 5% B. subtilis as adjuvant can also enhance the humoral response (p < 0.05). Taken together, oral administrated BSA nanocapsule can effectively induce humoral immune response in grouper and provides the possibility for future development of oral vaccine technology.
Advisors/Committee Members: Nen%20Chen%22%29&pagesize-30">
Ching-
Nen Chen (chair),
Chung-Da Yang (chair),
Jiin-Tsuey Cheng (chair),
Chen-Chih Kao (committee member).
Subjects/Keywords: humoral immunity; oral administration antigen; nanocapsule; poly-alkylcyanoacrylate; Epinephelus coioides
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APA ·
Chicago ·
MLA ·
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CSE |
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APA (6th Edition):
Lin, J. (2013). The effect of oral administrated protein nanocapsule on inducing humoral immunity in grouper, Epinephelus coioides. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0623113-170905
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lin, Jian-Bang. “The effect of oral administrated protein nanocapsule on inducing humoral immunity in grouper, Epinephelus coioides.” 2013. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0623113-170905.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lin, Jian-Bang. “The effect of oral administrated protein nanocapsule on inducing humoral immunity in grouper, Epinephelus coioides.” 2013. Web. 18 Apr 2021.
Vancouver:
Lin J. The effect of oral administrated protein nanocapsule on inducing humoral immunity in grouper, Epinephelus coioides. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0623113-170905.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lin J. The effect of oral administrated protein nanocapsule on inducing humoral immunity in grouper, Epinephelus coioides. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0623113-170905
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
4.
Chiu, Chia-Sheng.
Environmental Stress Tolerance of Aerial Microalgae from Dongsha Island and Their Potential Mechnaisms.
Degree: Master, Department of Oceanography, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0009117-160845
► It has been reported that microalgae can be found in the air, but aerial microalgae have not been investigated seriously. Microalgae lives in watery and…
(more)
▼ It has been reported that microalgae can be found in the air, but aerial microalgae have not been investigated seriously. Microalgae lives in watery and moist environments. When dispersed in the air, these cells have to tolerate drought stress, UV radiation, and either high or low temperature in order to survive. For the cells that can survive these environmental stresses, they have a chance to quickly expand their habitats by using wind as a means to spread. To investigate what microalgal species can survive these stresses and what mechanisms they use, four new species of aerial microalgae were isolated on Dongsha Island in South China Sea in the seasonal northeast wind. Based on 18S rDNA and ITS sequences and morphological analyses, the four new species were named Scenedesmus sp. DSA1, Coelastrella sp. DSA2, Coelastrella sp. DSA3 and Desmodesmus sp. DSA6, which are members in the family Scenedesmaceae. Their tolerance to environmental stresses were assessed, and the potential mechanisms were studied. The cell wall thickness of the four species and two controls positively correlates to their UV tolerance. The tolerance to drought and low temperature of DSA1, DSA2 and DSA3 is extremely high. Freeze-drying (lyophilization) could not kill these cells, and this phenomenon could not be explained by water content in the dried cells or their antioxidation capacities in these cells. Thermotolerance of DSA1, DSA2 and DSA6 is among the highest in the literature. These cells in liquid culture could survive at 50 â. The carotenoid levels in the stressed DSA2 and DSA3 cells were higher than in the DSA1 cells, followed by that in the DSA6 cells. These carotenoids contained astaxanthin, adonirubin, lutein,canthaxanthin, and ï¢-carotene analyzed by using HPLC.
Advisors/Committee Members: Yu-Chi Chen (chair), Nen%20Chen%22%29&pagesize-30">
Ching-
Nen Chen (committee member),
Yi-Min Chen (chair).
Subjects/Keywords: drought stress; UV stress; heat stress; low temperature stress; microalgae
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Chiu, C. (2017). Environmental Stress Tolerance of Aerial Microalgae from Dongsha Island and Their Potential Mechnaisms. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0009117-160845
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chiu, Chia-Sheng. “Environmental Stress Tolerance of Aerial Microalgae from Dongsha Island and Their Potential Mechnaisms.” 2017. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0009117-160845.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chiu, Chia-Sheng. “Environmental Stress Tolerance of Aerial Microalgae from Dongsha Island and Their Potential Mechnaisms.” 2017. Web. 18 Apr 2021.
Vancouver:
Chiu C. Environmental Stress Tolerance of Aerial Microalgae from Dongsha Island and Their Potential Mechnaisms. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0009117-160845.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chiu C. Environmental Stress Tolerance of Aerial Microalgae from Dongsha Island and Their Potential Mechnaisms. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0009117-160845
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
5.
Tsen, Chun-lin.
Distribution of unicellar diazotrophs and its relationship with environmental factors in the South China Sea â a whole-cell immunolocalization study.
Degree: Master, Marine Biotechnology and Resources, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615115-162133
► The purposes of this study were to investigate (1) the seasonal and spatial dynamics of cell densities of unicellular N2-fixing organisms (diazotrophs) in the South…
(more)
▼ The purposes of this study were to investigate (1) the seasonal and spatial dynamics of cell densities of unicellular N2-fixing organisms (diazotrophs) in the South China Sea; and (2) environmental factors relating to its dynamics. Four cruises, including one in the late spring, one in summer and two in winter were conducted between August 2011 and December 2013 in the area between 20°-22° N and 116°123° E. Samples were collected from surface to 200m in each station. In addition, nutrient enrichment experiments were carried out, including addition of 0.5μM Glucose + 0.5μM Mannitol (+DOC 1μM)ã0.4μM NaNO3 (+N)ã0.1μM KH2PO4 (+P)ã50nM FeCl3 + 20μM EDTA (+Fe) and Dust(+Dust). Unicellular diazotrophs were identified by the whole cell immunofluorescent method, which detected the existence of nitrogenase in the cells. Number of the cell were counted under a fluorescent microscope. Unicellular diazotrophs were grouped according to cell shapes (coccoid(C) and rod(R)), and cell sizes (0.2-1.5μm, 1.5-2μm, > 2μm). In order to compare with the features of the known groups of unicellular diazotrophic cyanobacteria, the group of 0.2-1.5μm C+R diazotrophs was regarded as UCYN-A alike; group of 1.5-2μm C and >2μm C were combined and regarded as UCYN-B alike; and group of 1.5-2μm R and >2μm R were combined and regarded as UCYN-C alike.
The results showed that cell densities of UCYN-A alike were higher in winter than other seasons, and higher on the shelf than the basin waters. The dynamics could be associated with the enhanced nutrients, including N, P, Fe and DOC, in winter / shelf. Nutrient enrichment experiments indicated that (conducted only in spring and winter) cell densities of UCYN-A alikes were increased significantly after addition of DOC, P or Fe, respectively. Addition of N also resulted to a significant increase in cell densities in spring and summer, but not in winter. This is likely because the nitrate concentrations in the surface waters in winter were higher than that of other two seasons.
Seasonal distributions of UCYN-B alikes and UCYN-C alikes were similar, both higher in spring and winter than summer. There was no significant pattern on their spatial distributions. Their cell densities were positively related to surface concentration of nitrate and phosphate, respectively. This indicated that high cell densities in winter and spring could be attributed to high ambient nutrient concentrations then. The result of nutrient enrichment experiments also supported this assertion. Addition of P or Fe (only conducted in spring and winter) enhanced the biomass significantly. Addition of N, similar to that of UCYN-A alikes, resulted in significant biomass increase only in summer and spring, not in winter. There was no significant change on cell densities after addition of DOC in spring, summer or winter, suggesting that unicellular diazotrophs in these size ranges did not use DOC, or the in situ DOC concentration was enough for their growth. Based on the results of seasonal/spatial distribution and the…
Advisors/Committee Members: Tse-Min Lee (chair), Yhu-ling Lee (committee member), nen%20Chen%22%29&pagesize-30">
Ching-
nen Chen (chair).
Subjects/Keywords: UCYN-C; nutrient concentration; UCYN-A; UCYN-B; South China Sea; unicellular diazotrophs
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APA ·
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APA (6th Edition):
Tsen, C. (2015). Distribution of unicellar diazotrophs and its relationship with environmental factors in the South China Sea â a whole-cell immunolocalization study. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615115-162133
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tsen, Chun-lin. “Distribution of unicellar diazotrophs and its relationship with environmental factors in the South China Sea â a whole-cell immunolocalization study.” 2015. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615115-162133.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tsen, Chun-lin. “Distribution of unicellar diazotrophs and its relationship with environmental factors in the South China Sea â a whole-cell immunolocalization study.” 2015. Web. 18 Apr 2021.
Vancouver:
Tsen C. Distribution of unicellar diazotrophs and its relationship with environmental factors in the South China Sea â a whole-cell immunolocalization study. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615115-162133.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tsen C. Distribution of unicellar diazotrophs and its relationship with environmental factors in the South China Sea â a whole-cell immunolocalization study. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615115-162133
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
6.
Lin, Yu-heng.
Study on raceway pond for microalgae culturing system.
Degree: Master, Marine Environment and Engineering, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0818114-212332
► The aim of this research is to use the FLOW-3D Computational Fluid Dynamics (CFD) software to simulate the raceway style microalgae cultivation system, which is…
(more)
▼ The aim of this research is to use the FLOW-3D Computational Fluid Dynamics (CFD) software to simulate the raceway style microalgae cultivation system, which is built at Microalgae Biotechnology and Engineering Laboratory, located in Annan Campus, and conducted by the University Center of Bioscience and Biotechnology of National Cheng Kung University.
The content of research is mainly divided into two parts, i.e., field test and numerical simulation: The field test is to verify the accuracy of numerical simulation of FLOW-3D and to adjust the settings of CFD parameters. The numerical simulation is to conduct a series of analysis for the system, such as the pathlines of fluid particles, average energy losses for up- and down-streamsâ ross-sections, mean velocity of flow field and energy conversion efficiency of waterwheel and so on. Based on the results of the flow patterns in the raceway channel and the efficiency of energy conversion of waterwheel, it could provide suggestions of the optimal design of the microalgae cultivation system and the direction of research.
The results of this research indicate that the curve of the raceway style microalgae cultivation system, due to poor design of channels, tends to have large areas of low-speed dead zones which cause the accumulation of algae. After conducting the design optimization of channels, it is expected to significantly reduce the coverage rate of dead zone and improve circulation efficiency of algae.
The waterwheel commonly used in the microalgae cultivation system is originated from the aeration waterwheel for fish pond, and the energy conversion rate is about 19% only. This research has reduced the gap between the lowest waterwheel blade and bottom of channel from present 7 cm to 2 cm and changed the blade angle from 0 degree to 45 degree. The result of the energy conversion efficiency of waterwheel increases to 26%. Although the effects of the phenomenon of flow patterns and the reduction of dead zone are significant, the waterwheel energy conversion efficiency is still relatively low.
Finally, this research suggests that if it wants to build a new microalgae cultivation system, the numerical simulation model should be carried out first to find the optimal design of system under the environment conditions and equipment limitations. For further studies, it recommends to explore other flow-making equipments or other style waterwheels for the raceway system to improve the energy conversion efficiency.
Advisors/Committee Members: Chih-Min Hsieh (chair), Tsuh-Sin Chang (chair), Chai-Cheug Huang (committee member), Jo-Shu Chang (chair), nen%20Chen%22%29&pagesize-30">
Ching-
nen Chen (chair).
Subjects/Keywords: waterwheel; dead zone; energy conversion efficiency; Raceway style microalgae cultivation system
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APA ·
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Manager
APA (6th Edition):
Lin, Y. (2014). Study on raceway pond for microalgae culturing system. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0818114-212332
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lin, Yu-heng. “Study on raceway pond for microalgae culturing system.” 2014. Thesis, NSYSU. Accessed April 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0818114-212332.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lin, Yu-heng. “Study on raceway pond for microalgae culturing system.” 2014. Web. 18 Apr 2021.
Vancouver:
Lin Y. Study on raceway pond for microalgae culturing system. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Apr 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0818114-212332.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lin Y. Study on raceway pond for microalgae culturing system. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0818114-212332
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations