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NSYSU
1.
Mao, Pu-Wei.
Suppressive Effects of Probiotics on Salmonella Induced Inflammation.
Degree: Master, Biological Sciences, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844 
► Salmonella infection in humans is commonly manifested as enterocolitis characterized by induction of epithelial secretion of pro-inflammatory cytokines and diarrhea, accompanied by infiltration of neutrophils…
(more)
▼ Salmonella infection in humans is commonly manifested as enterocolitis characterized by induction of epithelial secretion of pro-inflammatory cytokines and diarrhea, accompanied by infiltration of neutrophils in the intestinal submucosa, which is a hall-mark of intestinal inflammation. The recruitment of neutrophils from circulation to the subepithelial region is facilitated by chemokines such as interleukin-8 (IL-8), which is known to be significantly induced upon bacterial entry by host epithelial cells.
Probiotics are able to be used in the prevention or treatment of certain types of in-flammatory bowel disease (IBD). Certain types of Lactobacillus strains are successful in modulating pro-inflammatory cytokine signaling, which in turn, reduce inflammation in the gastrointestinal tract.
In this study, we used the human intestinal cell line HCT 116 as an intestinal epithelial model, and the human leukemic monocyte cell line THP-1 as a leukocyte model, to determine the effects of four arbitrarily chosen strains of Lactobacillus probiotics on inflammation caused by Salmonella infection. The Salmonella strain used in the study is the wild-type SL1344, grown under anaerobic conditions to late log phase to maximize invasion phenotype. The four probiotic strains are Lactobacillus acidophilus, Lactoba-cillus rhamnosus, Lactobacillus paracasei, and Lactobacillus delbrueckii.
Our results show that all four strains of probiotics were able to sporadically reduce mRNA expression of the three tested cytokines, but only Lactobacillus paracasei was able to consistently lower IL-8 expression. Our experimental results had shown in infection tests that upon Salmonella infection, the most acute response is seen in the upregu-lation of IL-8 expression, suggesting a possible relation between inflammation induced by bacterial infection and IL-8 induction. However, invasion assays show that Lactoba-cillus paracasei did not significantly reduce the amount of intracellular bacteria. In-stead, only Lactobacillus rhamnosus, which did not consistently suppress IL-8, signifi-cantly reduced bacterial invasion. These findings suggest that various probiotics sup-press inflammation through different mechanisms, and that the probiotic Lactobacillus rhamnosus is able to protect intestinal epithelial cells by rendering them less suscepti-ble to Salmonella invasion.
Advisors/Committee Members: I-Fei Huang (chair), Chih-Wen Shu (chair), Lin%22%29&pagesize-30">
Chen Chun-
Lin (committee member).
Subjects/Keywords: Salmonella; pro-inflammatory cytokine; inflammation; Lactobacillus; Probiotics; anti-inflammatory; intra-cellular pathogen
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APA (6th Edition):
Mao, P. (2016). Suppressive Effects of Probiotics on Salmonella Induced Inflammation. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mao, Pu-Wei. “Suppressive Effects of Probiotics on Salmonella Induced Inflammation.” 2016. Thesis, NSYSU. Accessed April 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mao, Pu-Wei. “Suppressive Effects of Probiotics on Salmonella Induced Inflammation.” 2016. Web. 17 Apr 2021.
Vancouver:
Mao P. Suppressive Effects of Probiotics on Salmonella Induced Inflammation. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Apr 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mao P. Suppressive Effects of Probiotics on Salmonella Induced Inflammation. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
2.
Li, Yu-wei.
The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice.
Degree: Master, Institute of Biomedical Sciences, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955
► Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor. Primary glioblastomas have a worse prognosis with a median survival of less than…
(more)
▼ Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor. Primary glioblastomas have a worse prognosis with a median survival of less than 2 years after diagnosis. The Hippo signaling transduction pathway plays multiple functions during organ development, adult tissues homeostasis and tumorigenesis, and its downstream mediator yes-associated protein 1 (YAP1) is known to function as a potent oncogene, which is amplified and overexpressed in various human cancers. The goal of this study is to study the role of Hippo pathway in GBM carcinogenesis. First, we selected primary murine glioblastoma cancer cell lines as an in vitro model system to study the functional roles of Hippo pathway in regulating the proliferation, adhesion and migration of GBM cells in vitro. Second, to identify the role of Hippo pathway in brain development, we selected GFAP drive Cre transgenic system to cross with conditional Mst1/Mst2 loxp mice to specific knockout Mst1/Mst2 (upstream kinases of Hippo pathway) expression in the glial cells during brain development. Moreover, to discovery the important function of the Hippo signaling involved in the development of GBM, we have established mouse models of GBM (activation of KrasG12D combined with p53 loss) for studying the critical roles of Hippo pathway in GBM progression. An understanding of the temporal regulation of Hippo during GBM progression and the identification of activation Hippo signaling combined p53 induced a transcriptional profilesâ with respect to upstream activators and downstream networks, which may play an important node in the design of clinical approaches targeting Hippo signaling for the treatment of GBM.
Advisors/Committee Members: Hung-Wen Huamg (chair), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair),
Kuang-hung Cheng (committee member).
Subjects/Keywords: Mouse model; Glioma; YAP1; MST1/2; Hippo pathway; Glioblastoma; Apoptosis; Verteporfin
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, Y. (2016). The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Yu-wei. “The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice.” 2016. Thesis, NSYSU. Accessed April 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Yu-wei. “The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice.” 2016. Web. 17 Apr 2021.
Vancouver:
Li Y. The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Apr 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li Y. The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
3.
Shen, Ying-Ying.
Generation and Characterization of Recombinant Adenovirus Encoding Irisin.
Degree: Master, Institute of Biomedical Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342
► Exercise represents one of the most effective approaches for control of obesity and metabolic syndromes. Irisin is a 112-residue myokine secreted by skeletal muscle through…
(more)
▼ Exercise represents one of the most effective approaches for control of obesity and metabolic syndromes. Irisin is a 112-residue myokine secreted by skeletal muscle through proteolytical cleavage from its precursor fibronectin type III domain containing 5 (FNDC5). Irisin stimulates brown fat-like development from white fat and thermogenesis through increasing mitochondria genesis and energy expenditure, thereby reducing body weight and insulin resistance. Because of its anti-obesity effects and evolutionary conservation, Irisin has been proposed as a promising therapeutic agent for metabolic diseases since its discovery in 2012. To combat the obesity syndrome, gene therapy approached may be required for long-term management of patients with metabolic diseases. Thus, the present study aims to generate the recombinant adenovirus vectors for Irisin production in various types of cells/organs, thereby evaluating their therapeutic potential and mechanism. Recombinant irisin was expressed and purified from E. coli with an apparent molecular weight of 14 kDa. The anti-irisin antibody was raised by periodical injection of recombinant irisin into rabbit and purified from serum using protein G Sepharose affinity chromatography. For gene delivery, adenovirus vector encoding FNDC5 (Ad-FNDC5) and irisin (Ad-irisin) were generated and purified by cesium chloride ultracentrifugation. To investigate the profile of FNDC5/irisin expression in different types of cells, endothelial EA.hy926, muscle C2C12, hepatocytes Clone-9, and embryonic kidney HEK293 cells were employed for adenovirus gene delivery. By immunoblot analysis and enzyme-linked immunoassay (ELISA), it was found that Ad-irisin effectively transduced and conferred irisin secretion in all four types of cells whereas Ad-FNDC5 evoked moderate irisin secretion only in C2C12 and Clone-9 cells. Infection with Ad-irisin enhanced the viability and migration of endothelial cells, supporting the pro-angiogenic function of irisin. Besides, Ad-irisin-infected hepatocytes exhibited elevated activities of AMPK/Akt and inhibition of PEPCK signaling, suggesting the role of irisin in gluconeogenesis in the livers. With the development of these irisin-based tools, future studies are warranted to elucidate the cellular function of irisin in different organs, thereby exploring the therapeutic potential of irisin therapy for various human diseases.
Advisors/Committee Members: Sheu, Jim Jinn-Chyuan (chair), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair),
Tai, Ming-Hong (committee member).
Subjects/Keywords: angiogenesis; gene therapy; obesity; myokines; Irisin; gluconeogenesis
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shen, Y. (2017). Generation and Characterization of Recombinant Adenovirus Encoding Irisin. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shen, Ying-Ying. “Generation and Characterization of Recombinant Adenovirus Encoding Irisin.” 2017. Thesis, NSYSU. Accessed April 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shen, Ying-Ying. “Generation and Characterization of Recombinant Adenovirus Encoding Irisin.” 2017. Web. 17 Apr 2021.
Vancouver:
Shen Y. Generation and Characterization of Recombinant Adenovirus Encoding Irisin. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Apr 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shen Y. Generation and Characterization of Recombinant Adenovirus Encoding Irisin. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
4.
Tseng , Shih-Ya.
Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model.
Degree: PhD, Biological Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714
► Critical limb ischemia (CLI) is an advanced form of peripheral artery disease in which the narrowing arteries limit blood supply to the lower extremities with…
(more)
▼ Critical limb ischemia (CLI) is an advanced form of peripheral artery disease in which the narrowing arteries limit blood supply to the lower extremities with resultant of resting pain and eventually, tissue loss. At present time, it is likely that proangiogenic stem cell therapy is anticipated as a promising therapeutic strategy in patients with CLI. However, a potential limitation of autologous cell therapy is that the insufficient number of stem cells were available the patients who may also suffer other problems. Therefore, how to generate enough autologous stem cells in vitro for future implantation application has become a major issue. Cilostazol is used as a vasodilating and anti-platelet aggregation drug clinically by increasing intracellular levels of cAMP. Our recent works and other reports have suggested that cilostazol may promote angiogenesis. Unfortunately, the effects of cilostazol on growth and differentiation of human early endothelial progenitor cells (EPCs) remain mostly unclear. In the current work, we explored the novel angiogenic effects of cilostazol on EPCs by using both in vitro and in vivo models. We found that human early EPCs treated with cilostazol significantly increase colony-forming units and enhanced differentiation of EPCs toward endothelial lineage. It was not only stimulated proliferation, migration, anti-apoptosis effect but also in vitro vascular tube formation through activation of SDF-1α /CXCR4/PI3K/Akt signaling pathway. In addition, Matrigel plug assay and mouse hind limb ischemia model also demonstrated that administration of a concomitant therapy with cilostazol and EPCs-treated mice were in vessel maturation higher, capillary significantly density and blood flow recovery, in comparison with either treatment alone. These results indicated that co-administration of cilostazol reinforced the autocrine effect of transplanted human early EPCs to provide a synergistic effect in angiogenesis through activation of SDF-1 α/CXCR4/PI3K/Akt signaling pathway. In clinical Implication, cilostazol plus EPCs treatment may be beneficial in improving EPC transplantation efficacy and enhancing vascular re-endothelialization in patients with critical limb ischemia.
Advisors/Committee Members: Wang, Hay-Yan (chair), Wang, Yang-Kao (chair), Cho, Chung-Lung (committee member), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair),
Chao, Ting-Hsing (committee member),
Li, Yi-Heng (chair).
Subjects/Keywords: Peripheral artery disease; Critical limb ischemia; Angiogenesis; Cilostazol; SDF-1α; Endothelial progenitor cells
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APA ·
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Export
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Manager
APA (6th Edition):
Tseng , S. (2017). Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714
Chicago Manual of Style (16th Edition):
Tseng , Shih-Ya. “Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model.” 2017. Doctoral Dissertation, NSYSU. Accessed April 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714.
MLA Handbook (7th Edition):
Tseng , Shih-Ya. “Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model.” 2017. Web. 17 Apr 2021.
Vancouver:
Tseng S. Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model. [Internet] [Doctoral dissertation]. NSYSU; 2017. [cited 2021 Apr 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714.
Council of Science Editors:
Tseng S. Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model. [Doctoral Dissertation]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714
NSYSU
5.
Wang, Chih-chiang.
The renal-protective effects of active substances from marine photosynthetic bacteria.
Degree: PhD, Marine Biotechnology and Resources, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948
► Chronic kidney disease (CKD) is a major burden worldwide. Oxidative stress and chronic inflammation play pivotal roles in CKD, especially CKD caused by diabetic nephropathy…
(more)
▼ Chronic kidney disease (CKD) is a major burden worldwide. Oxidative stress and chronic inflammation play pivotal roles in CKD, especially CKD caused by diabetic nephropathy (DN). End-stage renal disease that needs treatment through hemodialysis is most commonly caused via DN. Antioxidants have been shown to be beneficial against DN. However, antioxidants are rarely used in clinical practice, probably due to the extremely high doses needed to achieve therapeutic effects. Therefore, possible side effects and cost limit the use of antioxidants for therapeutic purposes. Recently, a novel compound LCG that displays anti-inflammatory activity was extracted from transformant Rhodobacter sphaeroides. LCG showed less toxicity and more potent anti-oxidative activity than lycopene, an antioxidant widely used as a nutritional supplement. Microbial carotenoids have many advantages over plant carotenoids. Carotenoid extraction from microorganisms is more effective and less expensive process. Fermentation is independent of the weather situation. The mutant strain displayed a 3.5-fold increase in carotenoid content, relative to the wild type.
The components of LCG were discovered via nuclear magnetic resonance (NMR) studies, these include spheroidenone, methoxyneurosporene, ξ-carotene, and neurosporene. Interestingly, no irritation response was seen on hamster skins after 30 days of treatment with 0.2% LCG, demonstrating that LCG has good biocompatibility. LCG reduced reactive oxygen species and epithelialâmesenchymal transition markers in H2O2-treated HK2 cells. Using a diabetic mouse model, orally administered LCG (200mg/kg) significantly reduced proteinuria, lowered blood sugar, and reduced insulin resistance after 24 hours. LycogenTM reduced apoptosis in diabetic mice measured via the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay from biopsies of renal tissue. A reduction in apoptosis can attenuate nephron loss and decrease proteinuria. LCG reduced phosphorylation of p38, which is probably how LCG presents an anti-inflammatory effect and reduces EMT. Due to less toxicity and more potent anti-oxidative activity than lycopene, LCG is a potential target for further investigation to confirm the benefits of high dose antioxidants treatment in DN.
Advisors/Committee Members: Wang, Hui-Min (chair), Pan, Chieh-Yu (chair), Chang, Chi-I (chair), Liu, Wang-ta (chair), Lin, Hsiu-Chin (chair), Hsu, Jue-Liang (chair), Chang, Li-Yu (chair), Wen, Zhi-Hong (committee member), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair).
Subjects/Keywords: diabetic nephropathy; oxidative stress; LCG; chronic kidney disease; antioxidants
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, C. (2017). The renal-protective effects of active substances from marine photosynthetic bacteria. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948
Chicago Manual of Style (16th Edition):
Wang, Chih-chiang. “The renal-protective effects of active substances from marine photosynthetic bacteria.” 2017. Doctoral Dissertation, NSYSU. Accessed April 17, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948.
MLA Handbook (7th Edition):
Wang, Chih-chiang. “The renal-protective effects of active substances from marine photosynthetic bacteria.” 2017. Web. 17 Apr 2021.
Vancouver:
Wang C. The renal-protective effects of active substances from marine photosynthetic bacteria. [Internet] [Doctoral dissertation]. NSYSU; 2017. [cited 2021 Apr 17].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948.
Council of Science Editors:
Wang C. The renal-protective effects of active substances from marine photosynthetic bacteria. [Doctoral Dissertation]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948
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