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NSYSU
1.
Lu, Chiao-Hui.
The pregnancy outcomes for human embryos cultured in single step medium and sequential medium.
Degree: Master, Biological Sciences, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546 
► Since Louise Joy Brown, the first baby of In Vitro Fertilization-Embryo Transfer (IVF-ET), was born in the United Kingdom in 1978, there had been over…
(more)
▼ Since Louise Joy Brown, the first baby of In Vitro Fertilization-Embryo Transfer (IVF-ET), was born in the United Kingdom in 1978, there had been over five million successful cases over the course of 35 years. There are two commercially available culture media in the market: Single Step Medium (Global Medium) and Sequential Medium (Sage Medium). The development of these two media had enhanced the quality of embryo and increased the pregnancy rate in IVF-ET. In this collection of 210 cases of IVF-ET, oocytes were fertilized by intracytoplasmic sperm injectionï¼ICSIï¼and randomly assigned to the two commercially-available culture media. The quantity and quality of embryos were observed and recorded for three consecutive days in order to compare the development of embryos in the two commercial culture medium. A fertilized rate of 75.5% in Global Medium and 77.5% in Sage Medium were observed on the first day. There was a higher number of cleaved cells in Global Medium and embryos of Grade 1 cells than those in Sage Medium both on the 2nd and the 3rd day, though no significant differences were noted in the pregnancy rates between the two samples. In the group of those over 38 years old, there was a higher number of cells in Global Medium, but exhibited no difference in either embryonic pattern or in the pregnancy rate. Though the development of commercial culture medium had enabled a longer cultivation, some cases still failed be cultured to the blastocytic stage due to other limitations. It had been attempted with morphologic selection in embryos by the 3rd day of culture, with a criteria of over six cells per embryo and minimal cell fragmentation, to reach compatible pregnancy rate of embryo implantation of the 5th day by conventional method. Our study indicated that the there was higher number of Grade 1 embryos with more than 6 cells and lower embryo fragmentation in Single Step Medium, thus yielded a better overall quality of embryo than Sequential Medium. It can be speculated, therefore, that the Single Step Medium would offer a valuable choice in patients with difficulty in reaching blastocytic stage by conventional method due to various factors.
Advisors/Committee Members: Lee Su-long (chair), Liu, Jong-Kang (committee member), Chang Chi-Chang (committee member), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair).
Subjects/Keywords: quality of embryo; embryo implantation; Sequential Medium; Single Step Medium; IVF; Embryo culture; In Vitro Fertilization
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APA (6th Edition):
Lu, C. (2014). The pregnancy outcomes for human embryos cultured in single step medium and sequential medium. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lu, Chiao-Hui. “The pregnancy outcomes for human embryos cultured in single step medium and sequential medium.” 2014. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lu, Chiao-Hui. “The pregnancy outcomes for human embryos cultured in single step medium and sequential medium.” 2014. Web. 05 Mar 2021.
Vancouver:
Lu C. The pregnancy outcomes for human embryos cultured in single step medium and sequential medium. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lu C. The pregnancy outcomes for human embryos cultured in single step medium and sequential medium. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
2.
Mao, Pu-Wei.
Suppressive Effects of Probiotics on Salmonella Induced Inflammation.
Degree: Master, Biological Sciences, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844
► Salmonella infection in humans is commonly manifested as enterocolitis characterized by induction of epithelial secretion of pro-inflammatory cytokines and diarrhea, accompanied by infiltration of neutrophils…
(more)
▼ Salmonella infection in humans is commonly manifested as enterocolitis characterized by induction of epithelial secretion of pro-inflammatory cytokines and diarrhea, accompanied by infiltration of neutrophils in the intestinal submucosa, which is a hall-mark of intestinal inflammation. The recruitment of neutrophils from circulation to the subepithelial region is facilitated by chemokines such as interleukin-8 (IL-8), which is known to be significantly induced upon bacterial entry by host epithelial cells.
Probiotics are able to be used in the prevention or treatment of certain types of in-flammatory bowel disease (IBD). Certain types of Lactobacillus strains are successful in modulating pro-inflammatory cytokine signaling, which in turn, reduce inflammation in the gastrointestinal tract.
In this study, we used the human intestinal cell line HCT 116 as an intestinal epithelial model, and the human leukemic monocyte cell line THP-1 as a leukocyte model, to determine the effects of four arbitrarily chosen strains of Lactobacillus probiotics on inflammation caused by Salmonella infection. The Salmonella strain used in the study is the wild-type SL1344, grown under anaerobic conditions to late log phase to maximize invasion phenotype. The four probiotic strains are Lactobacillus acidophilus, Lactoba-cillus rhamnosus, Lactobacillus paracasei, and Lactobacillus delbrueckii.
Our results show that all four strains of probiotics were able to sporadically reduce mRNA expression of the three tested cytokines, but only Lactobacillus paracasei was able to consistently lower IL-8 expression. Our experimental results had shown in infection tests that upon Salmonella infection, the most acute response is seen in the upregu-lation of IL-8 expression, suggesting a possible relation between inflammation induced by bacterial infection and IL-8 induction. However, invasion assays show that Lactoba-cillus paracasei did not significantly reduce the amount of intracellular bacteria. In-stead, only Lactobacillus rhamnosus, which did not consistently suppress IL-8, signifi-cantly reduced bacterial invasion. These findings suggest that various probiotics sup-press inflammation through different mechanisms, and that the probiotic Lactobacillus rhamnosus is able to protect intestinal epithelial cells by rendering them less suscepti-ble to Salmonella invasion.
Advisors/Committee Members: I-Fei Huang (chair), Chih-Wen Shu (chair), Lin%22%29&pagesize-30">
Chen Chun-
Lin (committee member).
Subjects/Keywords: Salmonella; pro-inflammatory cytokine; inflammation; Lactobacillus; Probiotics; anti-inflammatory; intra-cellular pathogen
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APA (6th Edition):
Mao, P. (2016). Suppressive Effects of Probiotics on Salmonella Induced Inflammation. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mao, Pu-Wei. “Suppressive Effects of Probiotics on Salmonella Induced Inflammation.” 2016. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mao, Pu-Wei. “Suppressive Effects of Probiotics on Salmonella Induced Inflammation.” 2016. Web. 05 Mar 2021.
Vancouver:
Mao P. Suppressive Effects of Probiotics on Salmonella Induced Inflammation. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mao P. Suppressive Effects of Probiotics on Salmonella Induced Inflammation. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
3.
Li, Yu-Ru.
To Explore β-Amyloid Induced Oxidative DNA Damage and Repair in Rat Primary Cortical Neurons.
Degree: Master, Biological Sciences, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1103115-165537
► Alzheimer's disease (AD) is one of the common form of age-related neurodegenerative diseases and the leading cause of senile dementia. In the beta-amyloid (Aβ) cascade…
(more)
▼ Alzheimer's disease (AD) is one of the common form of age-related neurodegenerative diseases and the leading cause of senile dementia. In the beta-amyloid (Aβ) cascade hypothesis, the aggregation of Aβ is a crucial event that produces amounts of oxidative stress contributed to neurotoxicity. In this study, our hypothesis is that Aβ-peptide aggregation enhances oxidative stress which leads to DNA damage and contributes to neurotoxicity. Therefore, increasing DNA repair efficiency and DNA integrity could rescue neuronal cells from Aβ-induced neuronal death. To test our hypothesis, we utilized lentivirus transduction to overexpress Aβ in rat primary cortical neurons. Results of Western blotting and dihydroethidium (DHE) staining have shown that expression of Aβ reached the peak in the first three days as well as the production of reactive oxygen species (ROS), then both Aβ and ROS levels were decreasing in the following day four to day seven. The DNA damage marker, phosphor-histone 2A (γH2AX), demonstrated that neuronal DNA injury was correlated to both levels of Aβ and ROS. Glucagon-like peptide-1 (GLP-1) is a growth factor which has been proved to have neuroprotective properties. After GLP-1 treatment, the production of Aβ and ROS was reduced, but γH2AX was still remaining in 72 hours. GLP-1 has been proved the effects of decreasing Aβ, inflammation and the improvement of recognition, learning and memory in animal model from previous studies. Although we did not see GLP-1 significantly reducing γH2AX, GLP-1 is still a potential drug involving DNA repair. In Alzheimerâs disease, to elevate DNA repair capability is also a important field to investigate in the future.
Advisors/Committee Members: Lin%22%29&pagesize-30">
Chen Chun-
Lin (committee member),
Lin%22%29&pagesize-30">Yang, Jenq-Lin (committee member),
Wu, Kay L.H. (chair),
Steve Leu (chair).
Subjects/Keywords: β-amyloid; Amyloid precursor protein; Alzheimer's disease; DNA repair; Neurotoxicity
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, Y. (2016). To Explore β-Amyloid Induced Oxidative DNA Damage and Repair in Rat Primary Cortical Neurons. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1103115-165537
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Yu-Ru. “To Explore β-Amyloid Induced Oxidative DNA Damage and Repair in Rat Primary Cortical Neurons.” 2016. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1103115-165537.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Yu-Ru. “To Explore β-Amyloid Induced Oxidative DNA Damage and Repair in Rat Primary Cortical Neurons.” 2016. Web. 05 Mar 2021.
Vancouver:
Li Y. To Explore β-Amyloid Induced Oxidative DNA Damage and Repair in Rat Primary Cortical Neurons. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1103115-165537.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li Y. To Explore β-Amyloid Induced Oxidative DNA Damage and Repair in Rat Primary Cortical Neurons. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1103115-165537
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
4.
Li, Yu-wei.
The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice.
Degree: Master, Institute of Biomedical Sciences, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955
► Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor. Primary glioblastomas have a worse prognosis with a median survival of less than…
(more)
▼ Glioblastoma multiforme (GBM) is the most aggressive and malignant primary brain tumor. Primary glioblastomas have a worse prognosis with a median survival of less than 2 years after diagnosis. The Hippo signaling transduction pathway plays multiple functions during organ development, adult tissues homeostasis and tumorigenesis, and its downstream mediator yes-associated protein 1 (YAP1) is known to function as a potent oncogene, which is amplified and overexpressed in various human cancers. The goal of this study is to study the role of Hippo pathway in GBM carcinogenesis. First, we selected primary murine glioblastoma cancer cell lines as an in vitro model system to study the functional roles of Hippo pathway in regulating the proliferation, adhesion and migration of GBM cells in vitro. Second, to identify the role of Hippo pathway in brain development, we selected GFAP drive Cre transgenic system to cross with conditional Mst1/Mst2 loxp mice to specific knockout Mst1/Mst2 (upstream kinases of Hippo pathway) expression in the glial cells during brain development. Moreover, to discovery the important function of the Hippo signaling involved in the development of GBM, we have established mouse models of GBM (activation of KrasG12D combined with p53 loss) for studying the critical roles of Hippo pathway in GBM progression. An understanding of the temporal regulation of Hippo during GBM progression and the identification of activation Hippo signaling combined p53 induced a transcriptional profilesâ with respect to upstream activators and downstream networks, which may play an important node in the design of clinical approaches targeting Hippo signaling for the treatment of GBM.
Advisors/Committee Members: Hung-Wen Huamg (chair), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair),
Kuang-hung Cheng (committee member).
Subjects/Keywords: Mouse model; Glioma; YAP1; MST1/2; Hippo pathway; Glioblastoma; Apoptosis; Verteporfin
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, Y. (2016). The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Yu-wei. “The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice.” 2016. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Yu-wei. “The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice.” 2016. Web. 05 Mar 2021.
Vancouver:
Li Y. The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li Y. The role of Mst1/Mst2 in early brain development and glioblastoma formation of the mice. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0628116-114955
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
5.
Chen, Chun-Yu.
The role of betulinic acid in modulation of membrane microdomains and TGF-β signaling.
Degree: Master, Biological Sciences, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0624115-165153
► Betulinic acid (BetA) is a phytochemical triterpenoid acid from bark extracts and is cytotoxic to cancer cells and tumors. The specific subcellular receptors that are…
(more)
▼ Betulinic acid (BetA) is a phytochemical triterpenoid acid from bark extracts and is cytotoxic to cancer cells and tumors. The specific subcellular receptors that are bound or deactivated by BetA have not been extensively investigated or identified. The goal of this study is to investigate the effect of BetA in transforming growth factor β (TGF-β) signaling. TGF-β is a key modulator in regulating cell proliferation and migration, it also involved in the process of cancer development and progression. TGF-β regulates tumor cell proliferation and invasion through a variety of Smad-dependent and -independent pathways. In most of cells, TGF-β receptors were located predominantly in lipid raft/caveolae microdomains, determined using sucrose density gradient ultracentrifugation and Western blot. BetA induces translocation of TGF-β receptors from lipid raft/caveolae to non-caveolae microdomains without changing total level of TGF-β receptors. Distribution of TGF-β receptors between lipid raft/caveolae- and clathrin-mediated endocytosis has been found to regulate TGF-β responsiveness. Most notably, BetA-induced TGF-β receptors translocation is rapid and correlate with the TGF-β-induced signaling. Here, we provide for the first the time evidence that BetA enhanced TGF-β signaling is associated with translocation of TGF-β receptors out of lipid raft/caveolae microdomains and a dramatic increase in the ability of TGF-β to stimulate Smad2 phosphorylation. Moreover, BetA-induced redistribution of TGF-β receptors also enhances TGF-β -induced PAI-1 reporter gene activation and growth inhibition in Mv1Lu cells. These results implicated that anticancer activities of BetA may be due, in part to the enhancing of TGF-β receptor signaling in non-caveolae microdomains in plasma membrane.
Advisors/Committee Members: Lin%20Yang%22%29&pagesize-30">Jenq-
Lin Yang (chair),
Yaw-Bin Huang (chair),
Lin%22%29&pagesize-30">Chen, Chun-Lin (committee member).
Subjects/Keywords: cholesterol; TGF-beta; caveolae; lipid-raft; Betulinic acid
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Chen, C. (2015). The role of betulinic acid in modulation of membrane microdomains and TGF-β signaling. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0624115-165153
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Chun-Yu. “The role of betulinic acid in modulation of membrane microdomains and TGF-β signaling.” 2015. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0624115-165153.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Chun-Yu. “The role of betulinic acid in modulation of membrane microdomains and TGF-β signaling.” 2015. Web. 05 Mar 2021.
Vancouver:
Chen C. The role of betulinic acid in modulation of membrane microdomains and TGF-β signaling. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0624115-165153.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen C. The role of betulinic acid in modulation of membrane microdomains and TGF-β signaling. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0624115-165153
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
6.
Chen, Ying-Pin.
Study the mechanisms of Euphol in the modulation of TGF-β responsiveness.
Degree: Master, Biological Sciences, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714115-141234
► Gastric cancer is a high prevalent carcinoma and the leading cause of cancer-related mortality in Taiwan. Transforming growth factor-β is one of growth factors family,…
(more)
▼ Gastric cancer is a high prevalent carcinoma and the leading cause of cancer-related mortality in Taiwan. Transforming growth factor-β is one of growth factors family, and involved in many biological processes leading to tumor formation, including cell proliferation, extracellular matrix secretion, cell adhesion, movement, differentiation and apoptosis. The TGF-β signaling its via two receptors complexes on the membrane, type I TGF-β receptor, TβRI and type II TGF-β receptor, TβRII, and activated downstream information by phosphorylation signaling mediator, protein Smad2/3, phosphorylated Smad2/3 was transferred to the nucleus to initiate transcription of downstream genes. Euphol is an euphane-type triterpene alcohol. It is isolated from the dichloromethane extract of Euphorbia, structurally similar to cholesterol which is a key component of lipid-raft microdomain on plasma membrane. It exhibits anti-viral and anti-inflammation activity, have higher cytotoxic in gastric cancer than normal cell, and will induce gastric cancer apoptosis pathway. However, the mechanisms and the potential of the anti-tumor properties of Euphol remain to be elucidated. The TGF-β receptor located in lipid-raft/caveolae microdomain stimulated by TGF-β will encounter lipid-raft/caveolae-mediated endocytosis, this endocytic pathway is a result of TGF-β receptors are transported to the lysosome for degradation. In contrast, TGF-β receptors which is located in non-lipid-raft region will be taken in the non-lipid-raft pathway. TGF-β receptors will transported into the early endosome, then going on signaling pathway. In this study, we found that TGF-β receptors were transferred from non-lipid raft to lipid-raft after Euphol treatment, this translocation increased degradation of TGF-β receptor and reduced the TGF-β signaling. Since the chemical structure of Euphol is similar to cholesterol. In our hypothesis, the cytotoxic of Euphol to gastric cancer may replace by cholesterol, then change the composed of protein and phospholipid, thereby influence the cell signaling of gastric cancer, then induce cancer cell death.
Advisors/Committee Members: Lin%22%29&pagesize-30">Yang, Jenq-
Lin (chair),
Lin%22%29&pagesize-30">Chen, Chun-Lin (committee member),
Huang, Yaw-Bin (chair).
Subjects/Keywords: cholesterol; lipid-raft; triterpene; Euphol; TGF-β
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chen, Y. (2015). Study the mechanisms of Euphol in the modulation of TGF-β responsiveness. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714115-141234
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Ying-Pin. “Study the mechanisms of Euphol in the modulation of TGF-β responsiveness.” 2015. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714115-141234.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Ying-Pin. “Study the mechanisms of Euphol in the modulation of TGF-β responsiveness.” 2015. Web. 05 Mar 2021.
Vancouver:
Chen Y. Study the mechanisms of Euphol in the modulation of TGF-β responsiveness. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714115-141234.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen Y. Study the mechanisms of Euphol in the modulation of TGF-β responsiveness. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714115-141234
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
7.
Yang, Pei-hua.
Study the mechanisms of Pentabromophenol in the modulation of TGF-β signaling.
Degree: Master, Biological Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715117-084334
► Pentabromophenol (PBP), a brominated flame retardant (BFR), is widely used in various consumer products. BFRs exert adverse health effects such as neurotoxic and endocrine-disrupting effects.…
(more)
▼ Pentabromophenol (PBP), a brominated flame retardant (BFR), is widely used in various consumer products. BFRs exert adverse health effects such as neurotoxic and endocrine-disrupting effects. In this study, we found that PBP suppressed TGF-β responsiveness by accelerating the turnover rate of TGF-β receptor. PBP suppressed TGF-β1-mediated cell migration, PAI-1 reporter gene activation and Smad2/3 activation in various type of cells, abolished TGF-β1-mediated repression of E-cadherin expression, as well as induction of vimentin expression along with Snail and Slug upregulation; thus, blocking the TGF-β1-mediated epithelial-to-mesenchymal transition (EMT) in A549 calls. TGF-β superfamily is a key player in the regulation of a wide variety of biological processes from development to pathogenesis including cell proliferation, migration, and the process of cancer development and progression. The in vitro results in this study provide a basis for studies of more detailed relationships between PBP and the modulation of TGF-β signaling. Because PBP is similar to other BFRs such as polybrominated diphenyl ethers (PBDEs), additional laboratory and mechanistic studies should be performed to examine BFRs as potential risk factors for tumorigenesis and other TGF-β-related diseases.
Advisors/Committee Members: Wu, Chang-Yi (chair), Chiu, Chien-Chih (chair), Wen, Zhi-Hong (chair), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (committee member).
Subjects/Keywords: BFR; EMT; Smad; TGF-β; PBP
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APA ·
Chicago ·
MLA ·
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APA (6th Edition):
Yang, P. (2017). Study the mechanisms of Pentabromophenol in the modulation of TGF-β signaling. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715117-084334
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yang, Pei-hua. “Study the mechanisms of Pentabromophenol in the modulation of TGF-β signaling.” 2017. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715117-084334.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yang, Pei-hua. “Study the mechanisms of Pentabromophenol in the modulation of TGF-β signaling.” 2017. Web. 05 Mar 2021.
Vancouver:
Yang P. Study the mechanisms of Pentabromophenol in the modulation of TGF-β signaling. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715117-084334.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yang P. Study the mechanisms of Pentabromophenol in the modulation of TGF-β signaling. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715117-084334
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
8.
Kao, Yu-Chen.
Study the effects of Dibromotyrosine Derivative in TGF-β responsiveness.
Degree: Master, Biological Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718117-194840
► The Transforming growth factor β1 (TGF-β1) is belong to transforming growth factor superfamily. Many tumor lesions process are related to TGF-β1, such as: cell proliferation,…
(more)
▼ The Transforming growth factor β1 (TGF-β1) is belong to transforming growth factor superfamily. Many tumor lesions process are related to TGF-β1, such as: cell proliferation, extracellular matrix secretion, cell attachment, movement, differentiation and apoptosis. TGF-β1 cell signaling via two protein receptors on membrane which are Type I TGF-β receptor (TβR-I) and Type II TGF-β receptor (TβR-II). TGF-β is activated that lead smad2/3 to phosphorylation, and p-Smad2/3 will transfer to nuclear than regulates the transcription of the target gene with other transcription factor. At cancer early stage, TGF-β will use inhibit cell proliferation and promote cell apoptosis to inhibit cancer growth, but at cancer late stage,instead, TGF-β will promote cancer cell growth, invasion, transfer and help it to escape the immune system attack. There are some small molecule inhibitors which can inhibit TGF-β cell signal transduction have great value at cancer research. Small molecule inhibitor is a powerful tool in research of signal transduction pathway interaction. In this study, we found (1â²R,5â²S,6â²S)-2-(3â²,5â²- dibromo-1â²,6â²-dihydroxy-4â²-oxocyclohex-2â²-enyl) acetonitrile (DT), a bromotyrosine derivate from Pseudoceratina sp., which inhibits the TβR-I serine/threonine kinase then inhibits TGF-β downstream cell signaling. We use such as: luciferase activity assay, western blotting, wound healing assay, in vitro ALK5 kinase assay etc. to know the effect of DT on TGF-β cell signaling, and use epithelial cells to study of the inhibitory effects of DT on TGF-β-induced Smad signaling and epithelial-to-mesenchymal transition. We also confirmed the new ALK5 inhibitor can effectively inhibit TGF-β stimulate smad2/3 to phosphorylation and inhibit smad2/3 transfer to nuclear. In addition, DT also can inhibit TGF-β stimulate epithelial-to-mesenchymal transition and A549 cell metastasis. Our study showed DT can apply to treatment of fibrotic diseases and cancer in the future.
Advisors/Committee Members: Wu, Chang-Yi (chair), Chien-Chih Chiu (chair), Wen Zhi-Hong (chair), Lin%22%29&pagesize-30">(
Chen,
Chun-
Lin (committee member).
Subjects/Keywords: TGF-β; bromotyrosine derivative; small molecular inhibitors; epithelial-to-mesenchymal transition
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kao, Y. (2017). Study the effects of Dibromotyrosine Derivative in TGF-β responsiveness. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718117-194840
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kao, Yu-Chen. “Study the effects of Dibromotyrosine Derivative in TGF-β responsiveness.” 2017. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718117-194840.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kao, Yu-Chen. “Study the effects of Dibromotyrosine Derivative in TGF-β responsiveness.” 2017. Web. 05 Mar 2021.
Vancouver:
Kao Y. Study the effects of Dibromotyrosine Derivative in TGF-β responsiveness. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718117-194840.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kao Y. Study the effects of Dibromotyrosine Derivative in TGF-β responsiveness. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0718117-194840
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
9.
Shen, Ying-Ying.
Generation and Characterization of Recombinant Adenovirus Encoding Irisin.
Degree: Master, Institute of Biomedical Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342
► Exercise represents one of the most effective approaches for control of obesity and metabolic syndromes. Irisin is a 112-residue myokine secreted by skeletal muscle through…
(more)
▼ Exercise represents one of the most effective approaches for control of obesity and metabolic syndromes. Irisin is a 112-residue myokine secreted by skeletal muscle through proteolytical cleavage from its precursor fibronectin type III domain containing 5 (FNDC5). Irisin stimulates brown fat-like development from white fat and thermogenesis through increasing mitochondria genesis and energy expenditure, thereby reducing body weight and insulin resistance. Because of its anti-obesity effects and evolutionary conservation, Irisin has been proposed as a promising therapeutic agent for metabolic diseases since its discovery in 2012. To combat the obesity syndrome, gene therapy approached may be required for long-term management of patients with metabolic diseases. Thus, the present study aims to generate the recombinant adenovirus vectors for Irisin production in various types of cells/organs, thereby evaluating their therapeutic potential and mechanism. Recombinant irisin was expressed and purified from E. coli with an apparent molecular weight of 14 kDa. The anti-irisin antibody was raised by periodical injection of recombinant irisin into rabbit and purified from serum using protein G Sepharose affinity chromatography. For gene delivery, adenovirus vector encoding FNDC5 (Ad-FNDC5) and irisin (Ad-irisin) were generated and purified by cesium chloride ultracentrifugation. To investigate the profile of FNDC5/irisin expression in different types of cells, endothelial EA.hy926, muscle C2C12, hepatocytes Clone-9, and embryonic kidney HEK293 cells were employed for adenovirus gene delivery. By immunoblot analysis and enzyme-linked immunoassay (ELISA), it was found that Ad-irisin effectively transduced and conferred irisin secretion in all four types of cells whereas Ad-FNDC5 evoked moderate irisin secretion only in C2C12 and Clone-9 cells. Infection with Ad-irisin enhanced the viability and migration of endothelial cells, supporting the pro-angiogenic function of irisin. Besides, Ad-irisin-infected hepatocytes exhibited elevated activities of AMPK/Akt and inhibition of PEPCK signaling, suggesting the role of irisin in gluconeogenesis in the livers. With the development of these irisin-based tools, future studies are warranted to elucidate the cellular function of irisin in different organs, thereby exploring the therapeutic potential of irisin therapy for various human diseases.
Advisors/Committee Members: Sheu, Jim Jinn-Chyuan (chair), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair),
Tai, Ming-Hong (committee member).
Subjects/Keywords: angiogenesis; gene therapy; obesity; myokines; Irisin; gluconeogenesis
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shen, Y. (2017). Generation and Characterization of Recombinant Adenovirus Encoding Irisin. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shen, Ying-Ying. “Generation and Characterization of Recombinant Adenovirus Encoding Irisin.” 2017. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shen, Ying-Ying. “Generation and Characterization of Recombinant Adenovirus Encoding Irisin.” 2017. Web. 05 Mar 2021.
Vancouver:
Shen Y. Generation and Characterization of Recombinant Adenovirus Encoding Irisin. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shen Y. Generation and Characterization of Recombinant Adenovirus Encoding Irisin. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722117-235342
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
10.
WU, HUI-CHUN.
Autophagy inducers modulated ATG4B expression in human brain tumor cells.
Degree: Master, Biological Sciences, 2018, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605118-111049
► Autophagy is a self-eating mechanism in cells through which damaged proteins and organelles are recruited to autophagosomes and fused with lysosome for their bulk degradation…
(more)
▼ Autophagy is a self-eating mechanism in cells through which damaged proteins and organelles are recruited to autophagosomes and fused with lysosome for their bulk degradation and recycling during nutrient deprivation. Dysregulation of autophagy is associated with various diseases, including cancer. ATG4B is a cysteine protease required for autophagy machinery. Recent reports have shown that elevated ATG4B promoted tumorigenesis, malignancy and drug resistance, suggesting ATG4B might modulate autophagy to facilitate tumor progression. However, the role of autophagy on ATG4B in cancer cells remains unknown. In this study, we found ATG4B protein level was decreased in glioma H4 and SHSY5Y cells during autophagy inducing conditions, including rapamycin and starvation. Moreover, autophagy inhibitors chloroquine or BafA1and proteosome inhibitor MG132 modestly recovered autophagy downregulated ATG4B in cells. Silencing ATG7 also partially recovered ATG4B protein level in cell treated with rapamycin, whereas it had no recovery effects in starved cells. Furthermore, mRNA level of ATG4B was decreased in rapamycin-treated H4 cells, but not starved cells. Luciferase fusion with 3âUTR of ATG4B as reporter assay was used to evaluate the effects of miRNA on ATG4B in cells during autophagy conditions. The luciferase activity was significantly decreased in H4 cells treated with rapamycin. However, the luciferase activity had little effects on recovery. The miR-34a was accordingly increased in the rapamycin-treated cells, while miR-34a was inhibited in BafA1 and CQ pretreated cells. Taken together, rapamycin may regulate autophagy and miR-34a to reduce ATG4B in glioma cells. Our results may shed a light on potential mechanisms of rapamycin on tumor suppression.
Advisors/Committee Members: Ming-Hong Tai (chair), Pei-Feng Liu (chair), Chih-Wen Shu (committee member), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (committee member).
Subjects/Keywords: Rapamycin; Chloroquine; Glioma; Autophagy; ATG4B
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
WU, H. (2018). Autophagy inducers modulated ATG4B expression in human brain tumor cells. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605118-111049
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
WU, HUI-CHUN. “Autophagy inducers modulated ATG4B expression in human brain tumor cells.” 2018. Thesis, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605118-111049.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
WU, HUI-CHUN. “Autophagy inducers modulated ATG4B expression in human brain tumor cells.” 2018. Web. 05 Mar 2021.
Vancouver:
WU H. Autophagy inducers modulated ATG4B expression in human brain tumor cells. [Internet] [Thesis]. NSYSU; 2018. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605118-111049.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
WU H. Autophagy inducers modulated ATG4B expression in human brain tumor cells. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0605118-111049
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
11.
Tsui, Kuan-Hao.
Effects of dehydroepiandrosterone supplementation on clinical outcomes and cumulus cells gene expression in women with poor ovarian response.
Degree: PhD, Biological Sciences, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0613115-115441
► The aim of the study was to investigate the effects of dehydroepiandrosterone (DHEA) supplementation on IVF (In vitro fertilization) outcomes and the gene expression of…
(more)
▼ The aim of the study was to investigate the effects of dehydroepiandrosterone (DHEA) supplementation on IVF (In vitro fertilization) outcomes and the gene expression of cumulus cells (CCs) in patients with poor ovarian response (POR). This was a prospective self-controlled study, including ten patients with POR. All women received DHEA supplementation (30 mg, t.i.d.) for around 3 months before starting the a new IVF cycle. Biochemical, ultrasound parameters and treatment outcomes were determined before and after DHEA therapy. Moreover, CCs were isolated to examine the gene expression level of nine genes, including Hyaluronan synthase (HAS2), Versican (VCAN), Thrombospondin 1 (THBS1), Runt-related transcription factor 2 (RUNX2), Chromobox homolog 3 (CBX3), Tripartite motif-containing 28 (TRIM28), B-cell lymphoma 2 (BCL2), BCL2-associated X protein (BAX), Ankyrin repeat domain 57 (ANKRD57), Syndecan 4 (SDC4), Activated leukocyte cell adhesion molecule (ALCAM), Gremlin 1 (GREM1), Prostaglandin-endoperoxide synthase 1 (PTGS1), Prostaglandin-endoperoxide synthase 2 (PTGS2), Sprouty homolog 4 (SPRY4), Coagulation factor II receptor-like 1 (F2RL1), Ribosomal protein S6 kinase, polypeptide 2 (RPS6KA2), solute carrier family 38, member 2 (SLC38A2), YTH domain familyï¼member 2 (YTHDF2), Cytochrome oxidase 17 (COX17), E3 ubiquitin protein ligase (UBR3), Krueppel-like factor 4 (KLF4), prostaglandin E receptor 2 (PTGER2), exostoses 1 (EXT1) by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). It showed significant changes in day 3 follicle-stimulating hormone (FSH), estradiol, antral follicle count (AFC) and Anti-Müllerian Hormone (AMH) (p < 0.0001, < 0.0001, < 0.05 and < 0.001, respectively), as well as number of oocytes retrieved, fertilized oocytes, day 3 embryos, transferred embryos (p < 0.0001, < 0.0001, < 0.05 and < 0.001, respectively) after DHEA therapy. In addition, the gene expression levels of extracellular matrix (ECM), including HAS2, VCAN, THBS1, showed significant increase after DHEA treatment. Although the gene expression levels of BCL2 and BAX, which are apoptotic genes, showed decrease; however, BCL2/BAX ratio elevated significantly, suggested that DHEA may play a role in anti-apoptosis. The study demonstrated that DHEA shows benefit for artificial reproductive outcomes and upregulates the gene expression of CCs in POR patients.
Advisors/Committee Members: Lin%22%29&pagesize-30">
Chen Chun-
Lin (chair),
Wen Zhi-Hong (chair),
Cheng Jiin-Tsuey (committee member),
Chang Shiuh-Young (chair),
Wang Peng-Hui (chair).
Subjects/Keywords: DHEA; cumulus cells; Dehydroepiandrosterone; diminished ovarian reserve
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tsui, K. (2015). Effects of dehydroepiandrosterone supplementation on clinical outcomes and cumulus cells gene expression in women with poor ovarian response. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0613115-115441
Chicago Manual of Style (16th Edition):
Tsui, Kuan-Hao. “Effects of dehydroepiandrosterone supplementation on clinical outcomes and cumulus cells gene expression in women with poor ovarian response.” 2015. Doctoral Dissertation, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0613115-115441.
MLA Handbook (7th Edition):
Tsui, Kuan-Hao. “Effects of dehydroepiandrosterone supplementation on clinical outcomes and cumulus cells gene expression in women with poor ovarian response.” 2015. Web. 05 Mar 2021.
Vancouver:
Tsui K. Effects of dehydroepiandrosterone supplementation on clinical outcomes and cumulus cells gene expression in women with poor ovarian response. [Internet] [Doctoral dissertation]. NSYSU; 2015. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0613115-115441.
Council of Science Editors:
Tsui K. Effects of dehydroepiandrosterone supplementation on clinical outcomes and cumulus cells gene expression in women with poor ovarian response. [Doctoral Dissertation]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0613115-115441
NSYSU
12.
Tseng , Shih-Ya.
Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model.
Degree: PhD, Biological Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714
► Critical limb ischemia (CLI) is an advanced form of peripheral artery disease in which the narrowing arteries limit blood supply to the lower extremities with…
(more)
▼ Critical limb ischemia (CLI) is an advanced form of peripheral artery disease in which the narrowing arteries limit blood supply to the lower extremities with resultant of resting pain and eventually, tissue loss. At present time, it is likely that proangiogenic stem cell therapy is anticipated as a promising therapeutic strategy in patients with CLI. However, a potential limitation of autologous cell therapy is that the insufficient number of stem cells were available the patients who may also suffer other problems. Therefore, how to generate enough autologous stem cells in vitro for future implantation application has become a major issue. Cilostazol is used as a vasodilating and anti-platelet aggregation drug clinically by increasing intracellular levels of cAMP. Our recent works and other reports have suggested that cilostazol may promote angiogenesis. Unfortunately, the effects of cilostazol on growth and differentiation of human early endothelial progenitor cells (EPCs) remain mostly unclear. In the current work, we explored the novel angiogenic effects of cilostazol on EPCs by using both in vitro and in vivo models. We found that human early EPCs treated with cilostazol significantly increase colony-forming units and enhanced differentiation of EPCs toward endothelial lineage. It was not only stimulated proliferation, migration, anti-apoptosis effect but also in vitro vascular tube formation through activation of SDF-1α /CXCR4/PI3K/Akt signaling pathway. In addition, Matrigel plug assay and mouse hind limb ischemia model also demonstrated that administration of a concomitant therapy with cilostazol and EPCs-treated mice were in vessel maturation higher, capillary significantly density and blood flow recovery, in comparison with either treatment alone. These results indicated that co-administration of cilostazol reinforced the autocrine effect of transplanted human early EPCs to provide a synergistic effect in angiogenesis through activation of SDF-1 α/CXCR4/PI3K/Akt signaling pathway. In clinical Implication, cilostazol plus EPCs treatment may be beneficial in improving EPC transplantation efficacy and enhancing vascular re-endothelialization in patients with critical limb ischemia.
Advisors/Committee Members: Wang, Hay-Yan (chair), Wang, Yang-Kao (chair), Cho, Chung-Lung (committee member), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair),
Chao, Ting-Hsing (committee member),
Li, Yi-Heng (chair).
Subjects/Keywords: Peripheral artery disease; Critical limb ischemia; Angiogenesis; Cilostazol; SDF-1α; Endothelial progenitor cells
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tseng , S. (2017). Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714
Chicago Manual of Style (16th Edition):
Tseng , Shih-Ya. “Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model.” 2017. Doctoral Dissertation, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714.
MLA Handbook (7th Edition):
Tseng , Shih-Ya. “Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model.” 2017. Web. 05 Mar 2021.
Vancouver:
Tseng S. Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model. [Internet] [Doctoral dissertation]. NSYSU; 2017. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714.
Council of Science Editors:
Tseng S. Cilostazol increases proangiogeneic functions of human early endothelial progenitor cells and hybrid therapy provides a synergistic treatment effect to hindlimb ischemia animal model. [Doctoral Dissertation]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0216117-100714
NSYSU
13.
Wang, Chih-chiang.
The renal-protective effects of active substances from marine photosynthetic bacteria.
Degree: PhD, Marine Biotechnology and Resources, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948
► Chronic kidney disease (CKD) is a major burden worldwide. Oxidative stress and chronic inflammation play pivotal roles in CKD, especially CKD caused by diabetic nephropathy…
(more)
▼ Chronic kidney disease (CKD) is a major burden worldwide. Oxidative stress and chronic inflammation play pivotal roles in CKD, especially CKD caused by diabetic nephropathy (DN). End-stage renal disease that needs treatment through hemodialysis is most commonly caused via DN. Antioxidants have been shown to be beneficial against DN. However, antioxidants are rarely used in clinical practice, probably due to the extremely high doses needed to achieve therapeutic effects. Therefore, possible side effects and cost limit the use of antioxidants for therapeutic purposes. Recently, a novel compound LCG that displays anti-inflammatory activity was extracted from transformant Rhodobacter sphaeroides. LCG showed less toxicity and more potent anti-oxidative activity than lycopene, an antioxidant widely used as a nutritional supplement. Microbial carotenoids have many advantages over plant carotenoids. Carotenoid extraction from microorganisms is more effective and less expensive process. Fermentation is independent of the weather situation. The mutant strain displayed a 3.5-fold increase in carotenoid content, relative to the wild type.
The components of LCG were discovered via nuclear magnetic resonance (NMR) studies, these include spheroidenone, methoxyneurosporene, ξ-carotene, and neurosporene. Interestingly, no irritation response was seen on hamster skins after 30 days of treatment with 0.2% LCG, demonstrating that LCG has good biocompatibility. LCG reduced reactive oxygen species and epithelialâmesenchymal transition markers in H2O2-treated HK2 cells. Using a diabetic mouse model, orally administered LCG (200mg/kg) significantly reduced proteinuria, lowered blood sugar, and reduced insulin resistance after 24 hours. LycogenTM reduced apoptosis in diabetic mice measured via the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay from biopsies of renal tissue. A reduction in apoptosis can attenuate nephron loss and decrease proteinuria. LCG reduced phosphorylation of p38, which is probably how LCG presents an anti-inflammatory effect and reduces EMT. Due to less toxicity and more potent anti-oxidative activity than lycopene, LCG is a potential target for further investigation to confirm the benefits of high dose antioxidants treatment in DN.
Advisors/Committee Members: Wang, Hui-Min (chair), Pan, Chieh-Yu (chair), Chang, Chi-I (chair), Liu, Wang-ta (chair), Lin, Hsiu-Chin (chair), Hsu, Jue-Liang (chair), Chang, Li-Yu (chair), Wen, Zhi-Hong (committee member), Lin%22%29&pagesize-30">
Chen,
Chun-
Lin (chair).
Subjects/Keywords: diabetic nephropathy; oxidative stress; LCG; chronic kidney disease; antioxidants
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Wang, C. (2017). The renal-protective effects of active substances from marine photosynthetic bacteria. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948
Chicago Manual of Style (16th Edition):
Wang, Chih-chiang. “The renal-protective effects of active substances from marine photosynthetic bacteria.” 2017. Doctoral Dissertation, NSYSU. Accessed March 05, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948.
MLA Handbook (7th Edition):
Wang, Chih-chiang. “The renal-protective effects of active substances from marine photosynthetic bacteria.” 2017. Web. 05 Mar 2021.
Vancouver:
Wang C. The renal-protective effects of active substances from marine photosynthetic bacteria. [Internet] [Doctoral dissertation]. NSYSU; 2017. [cited 2021 Mar 05].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948.
Council of Science Editors:
Wang C. The renal-protective effects of active substances from marine photosynthetic bacteria. [Doctoral Dissertation]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0506117-163948
. 
Open Access Theses and Dissertations