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1.
El-Soda, M.
Genetic analysis of drought stress response in Arabidopsis thaliana and Brassica rapa.
Degree: 2013, NARCIS
URL: http://library.wur.nl/WebQuery/wurpubs/443569 
;
urn:nbn:nl:ui:32-443569
;
urn:nbn:nl:ui:32-443569
;
http://library.wur.nl/WebQuery/wurpubs/443569
► Drought is the major abiotic stress affecting plant growth and limiting crop productivity worldwide. Plants have evolved three adaptive strategies, drought escape, drought avoidance…
(more)
▼ Drought is the major abiotic stress affecting plant growth and limiting crop productivity worldwide. Plants have evolved three adaptive strategies, drought escape, drought avoidance and drought tolerance, to cope with drought. Knowledge on how Quantitative Trait Loci (QTL), or genes underlying these strategies interact with their environments will significantly increase our understanding and the success of breeding for drought tolerance. This thesis focused on phenotyping shoot and root traits of
A. thaliana and
B. rapa grown on sand and in greenhouses,to further understand how plants can adapt to natural drought stress. In chapter 2, an already existing ArabidopsisRIL population was selected based on the differential root drought response of the two parental lines, Sha and Col, to be evaluated under different water regimes. Chapter 3 illustrated the use of GWAS in identifying candidate genes that are associated with pant response to drought.. In order to apply the same methodology in crop breeding, chapter 4 introduces a contribution to the genetic mapping of a new
B. rapa RIL population, consisting of 160 lines and genotyped with 270 different markers was achieved. The morphological and physiological responses of this population to drought was evaluated in chapter 5. The results presented in the present thesis demonstrate that QxE is an important component of the genetic variance and can play a great role in improving drought tolerance in future breeding programs. In general, several QTL and SNPs were mapped either with main effect or with interaction with environments QxE. Many of the mapped QTL showed conditional neutrality and antagonestic pleiotropy.
Advisors/Committee Members: Wageningen University, Maarten Koornneef, Mark Aarts.
Subjects/Keywords: brassica campestris; arabidopsis thaliana; droogte; droogteresistentie; stressreactie; genetische analyse; genotypen; genotype-milieu interactie; Genetica (algemeen); Plantenfysiologie; brassica campestris; arabidopsis thaliana; drought; drought resistance; stress response; genetic analysis; genotypes; genotype environment interaction; Genetics (General); Plant Physiology
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APA (6th Edition):
El-Soda, M. (2013). Genetic analysis of drought stress response in Arabidopsis thaliana and Brassica rapa. (Doctoral Dissertation). NARCIS. Retrieved from http://library.wur.nl/WebQuery/wurpubs/443569 ; urn:nbn:nl:ui:32-443569 ; urn:nbn:nl:ui:32-443569 ; http://library.wur.nl/WebQuery/wurpubs/443569
Chicago Manual of Style (16th Edition):
El-Soda, M. “Genetic analysis of drought stress response in Arabidopsis thaliana and Brassica rapa.” 2013. Doctoral Dissertation, NARCIS. Accessed January 21, 2021.
http://library.wur.nl/WebQuery/wurpubs/443569 ; urn:nbn:nl:ui:32-443569 ; urn:nbn:nl:ui:32-443569 ; http://library.wur.nl/WebQuery/wurpubs/443569.
MLA Handbook (7th Edition):
El-Soda, M. “Genetic analysis of drought stress response in Arabidopsis thaliana and Brassica rapa.” 2013. Web. 21 Jan 2021.
Vancouver:
El-Soda M. Genetic analysis of drought stress response in Arabidopsis thaliana and Brassica rapa. [Internet] [Doctoral dissertation]. NARCIS; 2013. [cited 2021 Jan 21].
Available from: http://library.wur.nl/WebQuery/wurpubs/443569 ; urn:nbn:nl:ui:32-443569 ; urn:nbn:nl:ui:32-443569 ; http://library.wur.nl/WebQuery/wurpubs/443569.
Council of Science Editors:
El-Soda M. Genetic analysis of drought stress response in Arabidopsis thaliana and Brassica rapa. [Doctoral Dissertation]. NARCIS; 2013. Available from: http://library.wur.nl/WebQuery/wurpubs/443569 ; urn:nbn:nl:ui:32-443569 ; urn:nbn:nl:ui:32-443569 ; http://library.wur.nl/WebQuery/wurpubs/443569
2.
Hassan, Z.
Functional analysis of zinc hyperaccumulation related genes of Noccaea (Thlaspi) caerulescens for phytoremediation purposes.
Degree: 2013, NARCIS
URL: http://library.wur.nl/WebQuery/wurpubs/433533
;
urn:nbn:nl:ui:32-433533
;
urn:nbn:nl:ui:32-433533
;
http://library.wur.nl/WebQuery/wurpubs/433533
► Zinc (Zn) and cadmium (Cd) are toxic metals that can cause serious soil contamination when present in excess. Especially Cd exposure is a threat…
(more)
▼ Zinc (Zn) and cadmium (Cd) are toxic metals that can cause serious soil contamination when present in excess. Especially Cd exposure is a threat to human health. Plants can potentially be used to clean-up the Zn- and/or Cd-contaminated soils in a technology called “Phytoremediation”. Two major requirements for this technology are the availability of plants that are accumulating Zn/Cd to high levels in their leaves and that are producing high biomass.
Noccaea caerulescens is a natural model Zn/Cd/Ni hyperaccumulator species that can accumulate up to 3% of Zn and 1% of Cd. Unfortunately it does not make a lot of biomass, and is thus unsuitable for phytoremediation. To overcome this limitation, higher biomass producing crops like tobacco can be genetically modified with genes from
N. caerulescens to induce metal tolerance and hyperaccumulation.
NcZNT1 and
NcMTP1 are metal transporter genes in
N. caerulescens. Zeshan Hassan has engineered tobacco to express both these genes, separately and in combination, to investigate their role in Zn, Cd accumulation and tolerance and to explore their potential in improving the phytoremediation capacity of plants. Transgenic tobacco lines were more tolerant to high Zn and Cd, leading to higher biomass compared to non-transformed controls, and they accumulated 2-4 fold higher Zn and 2-3.5 fold higher Cd when grown in artificial nutrient solution systems. More importantly they also did so when grown in soil collected from a metal-contaminated site. One of the conclusions of Zeshan Hassan’s PhD thesis is that both
NcZNT1 and
NcMTP1 genes are important for Zn and Cd tolerance and accumulation and that transgenic tobacco expressing these genes have enhanced phytoremediation capacity, which could lead to interesting future applications.
Advisors/Committee Members: Wageningen University, Maarten Koornneef, Mark Aarts.
Subjects/Keywords: thlaspi caerulescens; zink; hyperaccumulerende planten; fytoremediatie; genen; genexpressie; bodemverontreiniging; Genetica (algemeen); thlaspi caerulescens; zinc; hyperaccumulator plants; phytoremediation; genes; gene expression; soil pollution; Genetics (General)
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APA (6th Edition):
Hassan, Z. (2013). Functional analysis of zinc hyperaccumulation related genes of Noccaea (Thlaspi) caerulescens for phytoremediation purposes. (Doctoral Dissertation). NARCIS. Retrieved from http://library.wur.nl/WebQuery/wurpubs/433533 ; urn:nbn:nl:ui:32-433533 ; urn:nbn:nl:ui:32-433533 ; http://library.wur.nl/WebQuery/wurpubs/433533
Chicago Manual of Style (16th Edition):
Hassan, Z. “Functional analysis of zinc hyperaccumulation related genes of Noccaea (Thlaspi) caerulescens for phytoremediation purposes.” 2013. Doctoral Dissertation, NARCIS. Accessed January 21, 2021.
http://library.wur.nl/WebQuery/wurpubs/433533 ; urn:nbn:nl:ui:32-433533 ; urn:nbn:nl:ui:32-433533 ; http://library.wur.nl/WebQuery/wurpubs/433533.
MLA Handbook (7th Edition):
Hassan, Z. “Functional analysis of zinc hyperaccumulation related genes of Noccaea (Thlaspi) caerulescens for phytoremediation purposes.” 2013. Web. 21 Jan 2021.
Vancouver:
Hassan Z. Functional analysis of zinc hyperaccumulation related genes of Noccaea (Thlaspi) caerulescens for phytoremediation purposes. [Internet] [Doctoral dissertation]. NARCIS; 2013. [cited 2021 Jan 21].
Available from: http://library.wur.nl/WebQuery/wurpubs/433533 ; urn:nbn:nl:ui:32-433533 ; urn:nbn:nl:ui:32-433533 ; http://library.wur.nl/WebQuery/wurpubs/433533.
Council of Science Editors:
Hassan Z. Functional analysis of zinc hyperaccumulation related genes of Noccaea (Thlaspi) caerulescens for phytoremediation purposes. [Doctoral Dissertation]. NARCIS; 2013. Available from: http://library.wur.nl/WebQuery/wurpubs/433533 ; urn:nbn:nl:ui:32-433533 ; urn:nbn:nl:ui:32-433533 ; http://library.wur.nl/WebQuery/wurpubs/433533
3.
Bagheri, H.
Genetic analysis of breeding-related traits in Brassica rapa.
Degree: 2009, NARCIS
URL: http://library.wur.nl/WebQuery/wurpubs/385594
;
urn:nbn:nl:ui:32-385594
;
urn:nbn:nl:ui:32-385594
;
http://library.wur.nl/WebQuery/wurpubs/385594
► Brassica rapa is an important crop with a variety of forms, and a wide distribution in the world. It is used as oil seed and…
(more)
▼ Brassica rapa is an important crop with a variety of forms, and a wide distribution in the world. It is used as oil seed and vegetable crop and a valuable source of diverse health-promoting metabolites. It also can serve as a model for genetic and molecular analysis in the Brassica genus, to which all rapes, kales and cabbages belong, as it has the smallest genome size and some genotypes with a rapid life cycle. Insertional mutagenesis using heterologous maize transposons has been a valuable tool for the identification and isolation of genes in Arabidopsis. Transposon-based activation tagging systems use a construct with constitutive enhancer elements that can cause transcriptional activation of flanking plant genes, which can result in dominant mutant phenotypes and subsequent isolation of the genes involved. Chapter 2 describes the action of an En/I activation tagging construct in B. rapa through Agrobacterium rhizogenes–mediated hairy root transformation. Successful transformation of this construct to B. rapa ssp. by A. tumefaciens was not achieved, probably due to the combination of an inefficient plant transformation and regeneration system, the length of the construct and most importantly the presence of the SU1 gene in the construct that appears to inhibit the regeneration of transformed shoots. As an alternative to the insertional mutagenesis approach to identify genetic loci that impact traits, there is a genetic approach based on quantitative trait locus (QTL) analysis. Segregating populations are needed to map QTLs for traits of interest. Chapter 3 describes the analysis of an F2 population derived from a cross between two distinct, but early flowering and self compatible, B. rapa genotypes, L58 and R-o-18. Amplified fragment length polymorphism (AFLP) markers together with simple sequence repeat (SSR) markers were used to genotype this F2 population and anchor the linkage map to the reference genetic map of B. rapa. Highly significant QTLs associated with the production of adventitious roots and the transformation competence to A. rhizogenes were detected, which will allow the selection of lines that are more efficient in transformation experiments. The analysis detected a strong QTL associated with seed coat color as well as QTLs for various morphological traits. To fix the recombination events as much as possible and to obtain an “immortal” mapping population, a recombinant inbred line (RIL) population was developed from this F2 population. Chapter 4 describes development of this RIL population, for which a genetic linkage map was constructed using the Illumina® BeadXpressTM genotyping platform of Keygene NV and additional SSR markers. Analysis revealed an additional QTL for seed coat colour as well QTL for pod shattering, carpel number, cuticular wax and seed vivipary. Chapter 5 describes the detection of QTLs related to primary and secondary metabolites in this RIL population. The two parental lines show clear differences in metabolite profile, which allowed the finding…
Advisors/Committee Members: Wageningen University, Maarten Koornneef, Mark Aarts.
Subjects/Keywords: brassica campestris; brassica; genetische analyse; loci voor kwantitatief kenmerk; kwantitatieve kenmerken; kenmerken; rhizobium; zaadkenmerken; zaden; genetische transformatie; plantenveredeling; Moleculaire genetica; Plantenveredeling en -genetica (algemeen); brassica campestris; brassica; genetic analysis; quantitative trait loci; quantitative traits; traits; rhizobium; seed characteristics; seeds; genetic transformation; plant breeding; Molecular Genetics; Plant Breeding and Genetics (General)
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APA ·
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APA (6th Edition):
Bagheri, H. (2009). Genetic analysis of breeding-related traits in Brassica rapa. (Doctoral Dissertation). NARCIS. Retrieved from http://library.wur.nl/WebQuery/wurpubs/385594 ; urn:nbn:nl:ui:32-385594 ; urn:nbn:nl:ui:32-385594 ; http://library.wur.nl/WebQuery/wurpubs/385594
Chicago Manual of Style (16th Edition):
Bagheri, H. “Genetic analysis of breeding-related traits in Brassica rapa.” 2009. Doctoral Dissertation, NARCIS. Accessed January 21, 2021.
http://library.wur.nl/WebQuery/wurpubs/385594 ; urn:nbn:nl:ui:32-385594 ; urn:nbn:nl:ui:32-385594 ; http://library.wur.nl/WebQuery/wurpubs/385594.
MLA Handbook (7th Edition):
Bagheri, H. “Genetic analysis of breeding-related traits in Brassica rapa.” 2009. Web. 21 Jan 2021.
Vancouver:
Bagheri H. Genetic analysis of breeding-related traits in Brassica rapa. [Internet] [Doctoral dissertation]. NARCIS; 2009. [cited 2021 Jan 21].
Available from: http://library.wur.nl/WebQuery/wurpubs/385594 ; urn:nbn:nl:ui:32-385594 ; urn:nbn:nl:ui:32-385594 ; http://library.wur.nl/WebQuery/wurpubs/385594.
Council of Science Editors:
Bagheri H. Genetic analysis of breeding-related traits in Brassica rapa. [Doctoral Dissertation]. NARCIS; 2009. Available from: http://library.wur.nl/WebQuery/wurpubs/385594 ; urn:nbn:nl:ui:32-385594 ; urn:nbn:nl:ui:32-385594 ; http://library.wur.nl/WebQuery/wurpubs/385594
4.
Talukdar, S.
Functional characterisation of three zinc transporters in Thlaspi caerulescens.
Degree: 2007, NARCIS
URL: http://library.wur.nl/WebQuery/wurpubs/355359
;
urn:nbn:nl:ui:32-355359
;
urn:nbn:nl:ui:32-355359
;
http://library.wur.nl/WebQuery/wurpubs/355359
► Heavy metal hyperaccumulation in plants is a poorly understood phenomenon. Transmembrane metal transporters are assumed to play a key role in this process. In the…
(more)
▼ Heavy metal hyperaccumulation in plants is a poorly understood phenomenon. Transmembrane metal transporters are assumed to play a key role in this process. In the research described in this thesis, genes encoding Zn transporters of Thlaspi<span style='mso-bidi-font-weight:bold'>caerulescens</span><span style='mso-bidi-font-weight:bold;mso-bidi-font-style:italic'>, a heavy metal hyperaccumulator plant,</span>are studied and compared to their orthologues in Arabidopsis thaliana<span style='mso-bidi-font-style:italic'>, a non-hyperaccumulator plant</span>.<o:p></o:p></span>The Tc<span style='mso-bidi-font-style:italic'>ZNT1</span> and Tc<span style='mso-bidi-font-style:italic'>ZNT2</span> genes of T. caerulescens are members of the Zrt, Irt ( ZIP) -like gene family and show 89% and 87% similarity with the At<span style='mso-bidi-font-style:italic'>ZIP4</span> and At<span style='mso-bidi-font-style:italic'>IRT3</span> genes of A. thaliana<span style='mso-bidi-font-style: italic'>,</span>respectively. AtZIP4 and AtIRT3 genes are induced exclusively by Zn-deficiency in Arabidopsis. In limiting Zn conditions both Arabidopsis and Thlaspi show characteristic symptoms of Zn deficiency manifested both in vegetative and reproductive tissues. However, in T. caerulescens , Tc<span style='mso-bidi-font-style:italic'>ZNT1</span> and Tc<span style='mso-bidi-font-style:italic'>ZNT2</span> are constitutively and highly expressed in roots, irrespective of the Zn concentration in the medium. This observation suggested a role for<span class=GramE>these</span>genes in Zn uptake and possibly in explaining the Zn hyperaccumulation trait in Thlaspi . The proteins are localized in the plasma membrane, conferring zinc uptake into the cytoplasm. Tc<span style='mso-bidi-font-style:italic'>ZTP1,</span> another Zn transporter gene shows 85% sequence similarity in its coding region with the At<span style='mso-bidi-font-style: italic'>ZAT</span><span style='mso-bidi-font-style:italic'>/ MTP1 of</span>A. thaliana and is assumed to be localized in the vacuolar membrane. This gene belongs to the Cation Diffusion Facilitator (CDF) family.<o:p></o:p></span>The responses of the over-expression lines of TcZNT1,TcZNT2 and TcZTP1 in A. thaliana<span class=GramE>were</span>investigated with respect to deficiency and toxicity of Zn, Fe and Cd.</span><span lang=EN-GB style='font-family:"Times New Roman";mso-ansi-language:EN-GB'>Over-expression of Tc</span><span lang=EN-US style='font-family:"Times New Roman";mso-ansi-language: EN-US'>ZNT1</span><span lang=EN-GB style='font-family:"Times New Roman"; mso-ansi-language:EN-GB'>showed higher Zn accumulation in A. thaliana , whereas this effect was small and not significant for TcZNT2.TcZNT1 over-expression lines also showed increased Cd accumulation.</span><span lang=EN-US style='font-family:"Times New Roman";mso-ansi-language: EN-US'>In addition, the TcZNT1, TcZNT2 and TcZNT1/TcZNT2 silenced T. caerulescens showed</span><span lang=EN-GB style='font-family:"Times New Roman"; mso-ansi-language:EN-GB'>reduced Zn…
Advisors/Committee Members: Wageningen University, Maarten Koornneef, Mark Aarts.
Subjects/Keywords: thlaspi; zink; zware metalen; actief transport; genen; genexpressie; homeostase; bioaccumulatie; Genetica (algemeen); thlaspi; zinc; heavy metals; active transport; genes; gene expression; homeostasis; bioaccumulation; Genetics (General)
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Talukdar, S. (2007). Functional characterisation of three zinc transporters in Thlaspi caerulescens. (Doctoral Dissertation). NARCIS. Retrieved from http://library.wur.nl/WebQuery/wurpubs/355359 ; urn:nbn:nl:ui:32-355359 ; urn:nbn:nl:ui:32-355359 ; http://library.wur.nl/WebQuery/wurpubs/355359
Chicago Manual of Style (16th Edition):
Talukdar, S. “Functional characterisation of three zinc transporters in Thlaspi caerulescens.” 2007. Doctoral Dissertation, NARCIS. Accessed January 21, 2021.
http://library.wur.nl/WebQuery/wurpubs/355359 ; urn:nbn:nl:ui:32-355359 ; urn:nbn:nl:ui:32-355359 ; http://library.wur.nl/WebQuery/wurpubs/355359.
MLA Handbook (7th Edition):
Talukdar, S. “Functional characterisation of three zinc transporters in Thlaspi caerulescens.” 2007. Web. 21 Jan 2021.
Vancouver:
Talukdar S. Functional characterisation of three zinc transporters in Thlaspi caerulescens. [Internet] [Doctoral dissertation]. NARCIS; 2007. [cited 2021 Jan 21].
Available from: http://library.wur.nl/WebQuery/wurpubs/355359 ; urn:nbn:nl:ui:32-355359 ; urn:nbn:nl:ui:32-355359 ; http://library.wur.nl/WebQuery/wurpubs/355359.
Council of Science Editors:
Talukdar S. Functional characterisation of three zinc transporters in Thlaspi caerulescens. [Doctoral Dissertation]. NARCIS; 2007. Available from: http://library.wur.nl/WebQuery/wurpubs/355359 ; urn:nbn:nl:ui:32-355359 ; urn:nbn:nl:ui:32-355359 ; http://library.wur.nl/WebQuery/wurpubs/355359
5.
Wu, J.
Paving the way for genetic improvement of zinc accumulation in Brassica rapa.
Degree: 2007, NARCIS
URL: http://library.wur.nl/WebQuery/wurpubs/358063
;
urn:nbn:nl:ui:32-358063
;
urn:nbn:nl:ui:32-358063
;
http://library.wur.nl/WebQuery/wurpubs/358063
► <i>Brassica rapa</i> L. comprises several vegetable crops, some of which are among the most important vegetables in China, serving as one of the main resources…
(more)
▼ <i>Brassica rapa</i> L. comprises several vegetable crops, some of which are among the most important vegetables in China, serving as one of the main resources of mineral nutrition for Chinese people. However, the knowledge on the genetics of micronutrient accumulation, including Zn, Fe and Mn, is lacking in <i>B. rapa</i>. By exploring the genetic potential for the improvement of Zn accumulation in <i>B. rapa</i> vegetables I expect to contribute to the reduction of micronutrient malnutrition in China. To characterize the natural variation of Zn accumulation and Zn response upon exposure to different Zn concentrations, I screened a large collection of germplasm representing nine cultivar groups covering the geographic distribution of <i>B. rapa</i> vegetables in China (Chapter 2). The result revealed that there was marked variation in accumulation of Zn (23.2-159.9 µg g
-1), Fe (60.3-350.1 µg g
-1) and Mn (20.9-53.3 µg g
-1) in<i> B. rapa</i>. Zn accumulation correlated with Fe or Mn accumulation both under normal and deficient Zn supply. No significant correlation was detected for the accumulation of these three elements with cultivar groups. A two-fold variation was found for dry-biomass based tolerance to Zn deficiency or excessive Zn. The wide natural variation provides a base for the genetic dissection by quantitative trait locus (QTL) analysis or for developing breeding programs for improved mineral content. QTL analysis is a powerful tool in dissection of complex genetic traits. A doubled haploid (DH) population developed from two Chinese cabbage (<i>B. rapa</i>. ssp. <i>pekinesis</i>) varieties was used for QTL analysis of the accumulation of 11 minerals in leaves and for tolerance to deficient or toxic Zn supplies (Chapter 3). The trait analysis showed significantly positive correlations between the leaf concentrations of the tested minerals, indicating that this must be taken into consideration in breeding programs, especially when toxic minerals (Cd, As) are involved. Seven QTLs were detected for Na, Mg, P, Mn, Zn and Sr leaf concentrations, with an explained variance ranging from 11.1 % to 18.2 % . The tolerance to Zn stress was evaluated by dry shoot biomass of plants grown under different Zn supplies. One common QTL was found affecting Shoot Dry Biomass (SDB) under normal, deficient and excessive Zn supply conditions. An additional QTL was detected for SDB only under Zn excess stress, with an explained variance of 13.0 %. The fact that no major QTL was detected indicates the complexity of the genetic control of the traits, probably involving many loci, each with a limited contribution to mineral accumulation in <i>B</i>. <i>rapa</i>. Furthermore this indicates that the genetic improvement of these traits by classical breeding will be very complicated, as it will require a combination of favourable alleles at many of the genes contributing to the traits. Screening additional populations for larger effect QTLs may provide an alternative. Metal transporters play important…
Advisors/Committee Members: Wageningen University, Maarten Koornneef, Mark Aarts, Xiaowu Wang.
Subjects/Keywords: brassica campestris; zink; genetische verbetering; germplasm; loci voor kwantitatief kenmerk; genen; genexpressie; Moleculaire genetica; brassica campestris; zinc; genetic improvement; germplasm; quantitative trait loci; genes; gene expression; Molecular Genetics
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APA ·
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MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wu, J. (2007). Paving the way for genetic improvement of zinc accumulation in Brassica rapa. (Doctoral Dissertation). NARCIS. Retrieved from http://library.wur.nl/WebQuery/wurpubs/358063 ; urn:nbn:nl:ui:32-358063 ; urn:nbn:nl:ui:32-358063 ; http://library.wur.nl/WebQuery/wurpubs/358063
Chicago Manual of Style (16th Edition):
Wu, J. “Paving the way for genetic improvement of zinc accumulation in Brassica rapa.” 2007. Doctoral Dissertation, NARCIS. Accessed January 21, 2021.
http://library.wur.nl/WebQuery/wurpubs/358063 ; urn:nbn:nl:ui:32-358063 ; urn:nbn:nl:ui:32-358063 ; http://library.wur.nl/WebQuery/wurpubs/358063.
MLA Handbook (7th Edition):
Wu, J. “Paving the way for genetic improvement of zinc accumulation in Brassica rapa.” 2007. Web. 21 Jan 2021.
Vancouver:
Wu J. Paving the way for genetic improvement of zinc accumulation in Brassica rapa. [Internet] [Doctoral dissertation]. NARCIS; 2007. [cited 2021 Jan 21].
Available from: http://library.wur.nl/WebQuery/wurpubs/358063 ; urn:nbn:nl:ui:32-358063 ; urn:nbn:nl:ui:32-358063 ; http://library.wur.nl/WebQuery/wurpubs/358063.
Council of Science Editors:
Wu J. Paving the way for genetic improvement of zinc accumulation in Brassica rapa. [Doctoral Dissertation]. NARCIS; 2007. Available from: http://library.wur.nl/WebQuery/wurpubs/358063 ; urn:nbn:nl:ui:32-358063 ; urn:nbn:nl:ui:32-358063 ; http://library.wur.nl/WebQuery/wurpubs/358063
6.
Mortel, van de, J.E.
Heavy metal tolerance and accumulation in Thlaspi caerulescens, a heavy metal hyperaccumulating plant species = Zware metalen tolerantie en accumulatie in Thlaspi caerulescens, een zware metalen hyperaccumulerende plantensoort.
Degree: 2007, NARCIS
URL: http://library.wur.nl/WebQuery/wurpubs/356781
;
urn:nbn:nl:ui:32-356781
;
urn:nbn:nl:ui:32-356781
;
http://library.wur.nl/WebQuery/wurpubs/356781
► Minerals are essential for humans, plants and animals and have an important micronutrient role in physiological and metabolic processes of plants. Next to this essential…
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▼ Minerals are essential for humans, plants and animals and have an important micronutrient role in physiological and metabolic processes of plants. Next to this essential role of minerals, they can also be very toxic when available to the plant in elevated amounts. Plants therefore need to keep very tight control over the intracellular mineral concentrations in a process called metal homeostasis. Although the metal homeostasis mechanisms are supposed to be universal within plants, there are plant species that can tolerate and even accumulate large amounts of metals without any sign of toxicity. <i>Thlaspi caerulescens</i> J. & C. Presl (Brassicaceae), a close relative of the plant reference species <i>Arabidopsis thaliana</i> (Arabidopsis), is one of these natural metal hyperaccumulator species. The overall aim of this project is to unravel the molecular genetic mechanism of heavy metal tolerance and hyperaccumulation of the metal hyperaccumulating plant species <i>T. caerulescens. </i>To achieve this goal heterologous transcript profiling experiments were performed, which involved comparative microarray hybridization experiments of the hyperaccumulator <i>T. caerulescens</i> and Arabidopsis. Arabidopsis is used as the reference species for heterologous transcript profiling because of the availability of genetic resources and the complete genome sequence. The micronutrient zinc has an essential role in physiological and metabolic processes in plants as a cofactor or structural element. <i>Thlaspi caerulescens</i> can accumulate up to 3% of zinc on a dry weight basis without any sign of toxicity. The question postulated here is if this has drastic effects on the zinc homeostasis mechanism. We examined in detail the transcription profiles of roots of Arabidopsis and <i>T. caerulescens </i>plants grown under deficient, sufficient and excess supply of zinc (<b>Chapter 2</b>). A total of 608 genes were detected in Arabidopsis and 352 in <i>T. caerulescens</i> that responded transcriptionally to changes in zinc supply. Only 14% of these genes were also zinc-responsive in Arabidopsis. When comparing Arabidopsis and <i>T. caerulescens </i>at comparable zinc exposures, over 2200 genes were significantly differentially expressed. While a large fraction of these genes are of yet unknown function, many genes with a different expression between Arabidopsis and <i>T. caerulescens</i> appear to function in metal homeostasis, in abiotic stress response and in lignin biosynthesis. The high expression of lignin biosynthesis genes corresponds to the deposition of lignin in the endodermis. Contrary to Arabidopsis roots, which have one endodermal cell layer, we found there are two endodermal layers in <i>T. caerulescens</i> roots. This extra physical barrier could enhance the control of metal fluxes in the plant, in addition to the higher expression of metal transporters in the root.<b/> Cadmium is a widespread, naturally occurring non-essential element that is toxic for plants in higher concentrations. In <b>chapter 3</b> we compare…
Advisors/Committee Members: Wageningen University, Maarten Koornneef, Mark Aarts, H. Schat.
Subjects/Keywords: thlaspi; arabidopsis thaliana; zware metalen; tolerantie; genexpressie; Genetica (algemeen); thlaspi; arabidopsis thaliana; heavy metals; tolerance; gene expression; Genetics (General)
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APA (6th Edition):
Mortel, van de, J. E. (2007). Heavy metal tolerance and accumulation in Thlaspi caerulescens, a heavy metal hyperaccumulating plant species = Zware metalen tolerantie en accumulatie in Thlaspi caerulescens, een zware metalen hyperaccumulerende plantensoort. (Doctoral Dissertation). NARCIS. Retrieved from http://library.wur.nl/WebQuery/wurpubs/356781 ; urn:nbn:nl:ui:32-356781 ; urn:nbn:nl:ui:32-356781 ; http://library.wur.nl/WebQuery/wurpubs/356781
Chicago Manual of Style (16th Edition):
Mortel, van de, J E. “Heavy metal tolerance and accumulation in Thlaspi caerulescens, a heavy metal hyperaccumulating plant species = Zware metalen tolerantie en accumulatie in Thlaspi caerulescens, een zware metalen hyperaccumulerende plantensoort.” 2007. Doctoral Dissertation, NARCIS. Accessed January 21, 2021.
http://library.wur.nl/WebQuery/wurpubs/356781 ; urn:nbn:nl:ui:32-356781 ; urn:nbn:nl:ui:32-356781 ; http://library.wur.nl/WebQuery/wurpubs/356781.
MLA Handbook (7th Edition):
Mortel, van de, J E. “Heavy metal tolerance and accumulation in Thlaspi caerulescens, a heavy metal hyperaccumulating plant species = Zware metalen tolerantie en accumulatie in Thlaspi caerulescens, een zware metalen hyperaccumulerende plantensoort.” 2007. Web. 21 Jan 2021.
Vancouver:
Mortel, van de JE. Heavy metal tolerance and accumulation in Thlaspi caerulescens, a heavy metal hyperaccumulating plant species = Zware metalen tolerantie en accumulatie in Thlaspi caerulescens, een zware metalen hyperaccumulerende plantensoort. [Internet] [Doctoral dissertation]. NARCIS; 2007. [cited 2021 Jan 21].
Available from: http://library.wur.nl/WebQuery/wurpubs/356781 ; urn:nbn:nl:ui:32-356781 ; urn:nbn:nl:ui:32-356781 ; http://library.wur.nl/WebQuery/wurpubs/356781.
Council of Science Editors:
Mortel, van de JE. Heavy metal tolerance and accumulation in Thlaspi caerulescens, a heavy metal hyperaccumulating plant species = Zware metalen tolerantie en accumulatie in Thlaspi caerulescens, een zware metalen hyperaccumulerende plantensoort. [Doctoral Dissertation]. NARCIS; 2007. Available from: http://library.wur.nl/WebQuery/wurpubs/356781 ; urn:nbn:nl:ui:32-356781 ; urn:nbn:nl:ui:32-356781 ; http://library.wur.nl/WebQuery/wurpubs/356781
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