You searched for +publisher:"Michigan Technological University" +contributor:("Bruce P. Lee")
.
Showing records 1 – 8 of
8 total matches.
No search limiters apply to these results.

Michigan Technological University
1.
Liu, Yuan.
DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY.
Degree: PhD, Department of Biomedical Engineering, 2017, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/352
► The structure of catechol is found in mussel adhesive proteins and contributed to both wet-resistant adhesion and cohesive curing of these proteins. A synthetic…
(more)
▼ The structure of catechol is found in mussel adhesive proteins and contributed to both wet-resistant adhesion and cohesive curing of these proteins. A synthetic nano-silicate, Laponite was incorporated into catechol-containing hydrogels and the hydrogel network-bound catechol formed strong reversible interfacial interaction with Laponite. The contribution of incorporated catechol-Laponite reversible interfacial interactions to the mechanics of hydrogels constructed by different strategies was studied. In the first strategy, Laponite and catechol were introduced into the double network hydrogel (DN) via the free radical co-polymerization of a catechol-containing monomer, backbone monomer, and crosslinker. The introduction of catechol-Laponite interactions significantly improved the compressive strength and toughness of DN without compromising the compliance of the hydrogel and enabled the DN’s ability to recover its mechanical properties during successive loading cycles. In the second strategy, Laponite was combined with an catechol-modified 4-armed poly(ethylene glycol)-based adhesive, which cured in the present of an oxidative catalyst, sodium periodate, to form an injectable naoncomposite tissue adhesive hydrogel. The addition of up to 2 wt% Laponite significantly reduced the cure time, enhanced the bulk mechanical and adhesive properties of the adhesive due to strong catechol-Laponite interfacial binding. Additionally, subcutaneous implantation result showed that incorporation of Laponite effectively promote cell infiltration into the nanocomposite hydrogel, providing a simple way to improve the bioactivity of a bio-inert, synthetic poly(ethylene glycol)-based adhesive. On the basis of the second strategy, higher concentration of Laponite was combined with catechol-modified 6- and 8-armed PEG-based adhesive to form a nanocomposite hydrogel without introducing additional oxidative catalyst in the third strategy. This hydrogel underwent unique dynamic crosslinking process. At early stage it recovered to its original stiffness immediately after failure induced by shear strain up to 1000% interactions and could be reshaped to adhere to the contour of tissue due to the catechol-Laponite interactions and loosely chemically crosslinked network structure, respectively. The hydrogel gradually transformed to a densely chemically crosslinked network meanwhile fixed its shape as tissue sealant. This dissertation provided an insight of exploiting mussel-inspired chemistry in designing a hydrogel with specific materials property.
Advisors/Committee Members: Bruce P. Lee.
Subjects/Keywords: hydrogel; mussel-inspired material; polymer-nanoparticle bonds; tissue adhesive; Biology and Biomimetic Materials; Biomedical Engineering and Bioengineering; Polymer and Organic Materials
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Liu, Y. (2017). DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/352
Chicago Manual of Style (16th Edition):
Liu, Yuan. “DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY.” 2017. Doctoral Dissertation, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etdr/352.
MLA Handbook (7th Edition):
Liu, Yuan. “DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY.” 2017. Web. 24 Jan 2021.
Vancouver:
Liu Y. DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY. [Internet] [Doctoral dissertation]. Michigan Technological University; 2017. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etdr/352.
Council of Science Editors:
Liu Y. DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY. [Doctoral Dissertation]. Michigan Technological University; 2017. Available from: https://digitalcommons.mtu.edu/etdr/352

Michigan Technological University
2.
Li, Yuting.
GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY.
Degree: MS, Department of Biomedical Engineering, 2015, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etds/999
► In this study, chemically crosslinked gelatin microgels were incorporated into dopamine-modified poly (ethylene glycol) (PEGDM) adhesive to form composite bioadhesive with simultaneously improved adhesive…
(more)
▼ In this study, chemically crosslinked gelatin microgels were incorporated into dopamine-modified poly (ethylene glycol) (PEGDM) adhesive to form composite bioadhesive with simultaneously improved adhesive property and bioactivity. Gelatin microgel, with an average diameter of 53.6±14.2μm, was prepared with water in oil emulsification method and chemically crosslinked with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Gelatin microgels were incorporated into PEGDM adhesive precursor solution at 1.5wt%, 3.75wt% and 7.5wt%. The cure time of adhesive reduced from 54 seconds to 37 seconds with increasing gelatin microgel content. Additionally, the incorporation of the gelatin microgel also increased the crosslinking density of the adhesive network as indicated by the reduced equilibrium water content and increased elastic modulus based on compression testing. The compliance of adhesive was not compromised with the increased crosslinking density, as the failure strain showed no significant decrease from the compression testing result. Results from oscillator rheometry indicated that both the storage and loss moduli of the adhesive increased with increasing microgel content, which suggested that the microgels increased both chemical and physical crosslinks in the adhesive architecture. The increased physical crosslink indicated increased energy dissipation ability of the adhesive. Lap shear adhesive test demonstrated that the addition of gelatin microgel enhanced the adhesive property of adhesive. The adhesive property was increased 1.5-2 fold after the addition of gelatin microgel. In the
in vitro degradation test, samples of different formulation groups degraded gradually under a similar rate after soaked in the phosphate buffer solution (pH=7.4) and incubated at 37°C. After 8 weeks samples were completely degraded. The
in vitro cell viability was tested with L929 mouse fibroblast and the results showed no cytotoxicity in each test formulation. The
in vitro cell attachment experiment revealed an enhanced cell attachment and spreading of primary rat dermal fibroblast on gelatin microgel containing PEGDM adhesive compared to the adhesive without gelatin microgel. The results of rat subcutaneous implantation revealed higher cell recruitment and collagen deposition compared with control adhesive group which has no gelatin microgel in structure. Cell infiltration was found in the pocket structure formed by the degradation of gelatin microgel. In conclusion, the incorporation of gelatin microgel presents a simple method to simultaneously enhance the adhesive property and bioactivity of bioadhesive.
Advisors/Committee Members: Bruce P. Lee.
Subjects/Keywords: Adhesive property; Bioactivity; Bioadhesive; Dopamine modified-poly(ethylene glycol); Gelatin microgel; Biomedical Engineering and Bioengineering
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, Y. (2015). GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY. (Masters Thesis). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etds/999
Chicago Manual of Style (16th Edition):
Li, Yuting. “GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY.” 2015. Masters Thesis, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etds/999.
MLA Handbook (7th Edition):
Li, Yuting. “GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY.” 2015. Web. 24 Jan 2021.
Vancouver:
Li Y. GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY. [Internet] [Masters thesis]. Michigan Technological University; 2015. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etds/999.
Council of Science Editors:
Li Y. GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY. [Masters Thesis]. Michigan Technological University; 2015. Available from: https://digitalcommons.mtu.edu/etds/999

Michigan Technological University
3.
Meng, Hao.
SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY.
Degree: PhD, Department of Biomedical Engineering, 2017, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/363
► Mussel adhesive moiety, catechol, has been utilized to design a wide variety of biomaterials. Oxidation of catechol to form highly reactive quinone is a…
(more)
▼ Mussel adhesive moiety, catechol, has been utilized to design a wide variety of biomaterials. Oxidation of catechol to form highly reactive quinone is a necessary step to further involve into different reaction pathway to form intermolecular crosslinking (hardening adhesive and adhere to tissue). However the biological responses of mussel inspired biomaterials associated with the by-products generated during oxidation of catechol has never been characterized. In previous studies, cytotoxic level of hydrogen peroxide (H
2O
2) was captured in the dopamine doped cell culture medium. However, correlated in vivo studies did not investigated in these studies due to the lack of desirable model systems work as dopamine carriers. Additionally, the biological responses to the H
2O
2 are highly dependent on H
2O
2 concentration. Lower concentration of H
2O
2 promoted wound healing whilst higher concentration of H
2O
2 killed bacteria. Therefore, model hydrogel system was modulated into different physical appearance (bulk, injectable and microgel) to determine H
2O
2 production pattern and its biological responses and antibacterial property. The model system 1 was using catechol modified non-degradable bulk hydrogel (polyacrylamide, PAAm) to study H
2O
2 production during autoxidation of the catechol. 10
2 −10
3 μM concentration of H
2O
2 generated from hydrogel bound catechol and resulted in
in vitro cytotoxicity and elevated
in vivo foreign body reaction. The model system 2 was using 4-armed polyethylene glycol polymer end-capped with dopamine (PEG-D4) to characterize the production of H
2O
2 during the oxidant-mediated oxidative crosslinking of catechol. The maximum of 40 μM H
2O
2 generated from catechol moieties at the first 6 hours but ceased to generate H
2O
2 after the crosslink finished. Lower concentration of H
2O
2 exhibited localized cell responses in the culture (cytotoxicity) and
in vivo (M2 macrophages differentiation). To maximum H
2O
2 production and diffusion, we developed a catechol modified recyclable microgel system (hydroxyethyl acrylamide, HEAA) which can be stored as dried powder and initiated H
2O
2 production only after mixing with liquid solutions (system 3). 10
3−5x10
3 μM of H
2O
2 was captured in microgels extract for 4 days and function as an effective bactericide agent. This dissertation addressed the H
2O
2 concentration from several micromolar to millimolar can be actively monitored to fit various types of applications.
Advisors/Committee Members: Bruce P. Lee.
Subjects/Keywords: Hydrogen peroxide; mussel inspired adhesive; catechol; biocompatibility; antimicrobial; Biomaterials; Molecular, Cellular, and Tissue Engineering; Other Biomedical Engineering and Bioengineering
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Meng, H. (2017). SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/363
Chicago Manual of Style (16th Edition):
Meng, Hao. “SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY.” 2017. Doctoral Dissertation, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etdr/363.
MLA Handbook (7th Edition):
Meng, Hao. “SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY.” 2017. Web. 24 Jan 2021.
Vancouver:
Meng H. SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY. [Internet] [Doctoral dissertation]. Michigan Technological University; 2017. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etdr/363.
Council of Science Editors:
Meng H. SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY. [Doctoral Dissertation]. Michigan Technological University; 2017. Available from: https://digitalcommons.mtu.edu/etdr/363

Michigan Technological University
4.
Narkar, Ameya R.
REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY.
Degree: PhD, Department of Biomedical Engineering, 2018, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/741
► Catecholic groups in mussel adhesive proteins transition from being strongly adhesive in a reduced state under acidic conditions to being weakly adhesive in an…
(more)
▼ Catecholic groups in mussel adhesive proteins transition from being strongly adhesive in a reduced state under acidic conditions to being weakly adhesive in an oxidized state under basic conditions. Here, we exploit this pH responsive behavior of catechol and demonstrate that its oxidation state can be manipulated by incorporation of boronic acid to facilitate reversible transitions between strong and weak adhesion. Our first approach involved the addition of 3- acrylamido phenylboronic acid (APBA) to dopamine methacrylamide (DMA) containing adhesives. The synthesized adhesives showed strong adhesion to quartz surface in an acidic medium (pH 3), while weak adhesion was observed on raising the pH to a basic value (pH 9), due to unavailability of catechol and boronic acid because of the formation of a reversible catechol-boronate complex. Boronic acid not only contributed to adhesion at an acidic pH, but also allowed the catechol to reversibly interact with the surface in response to changing pH. In our second study, we demonstrated that addition of an anionic monomer, acrylic acid (AAc), preserved the reduced and adhesive state of catechol even at a neutral to mildly basic pH, while the addition of a cationic monomer, N-(aminopropyl) methacrylamide hydrochloride, led to the oxidized and weak adhesive state at higher basic pH values. This was due to the buffering of local pH offered by the incorporation of the ionic species, which affected the oxidation state of catechol. Although the ideal pH for formation of the complex is 9, it readily forms at neutral to mildly basic pH, leading to decreased adhesion and limiting the adhesive’s application in physiological and marine pH environments. In our third 2 approach, adding elevated amounts of AAc to smart adhesives consisting of DMA and APBA led to strong adhesion to quartz substrate at neutral to mildly basic pH. Moreover, the complex formed at pH 9 remained reversible and the interfacial binding could be tuned by changing the pH during successive contact cycles. pH 3 was required to break the complex and recover the strong adhesive property. Bulk adhesives analyzed in our first three approaches needed extended periods of incubation (up to 30 min) to switch between their adhesive and non-adhesive states. This is because infiltration of the pH media into the bulk polymer is limited by the slow process of diffusion. Finally, we fabricated a hybrid adhesive which was composed of gecko-inspired microstructured PDMS pillars (aspect ratios of 0.4-2) coated with the smart adhesive that we developed in our first approach. By tuning the aspect ratio of the bare templates, hybrid structures that showed strong, elevated adhesion at pH 3, were obtained. The increased adhesion was attributed to contact-splitting effects due to the micropatterning combined with the interfacial binding of the smart adhesive. On the other hand, formation of the complex, and the associated swelling of the adhesive together contributed to a significant decrease in adhesion at pH 9. Additionally, the…
Advisors/Committee Members: Bruce P. Lee.
Subjects/Keywords: Mussel Adhesion; Catechol; Reversible adhesion; Smart Adhesive; Rapid adhesion; Biomaterials
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Narkar, A. R. (2018). REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/741
Chicago Manual of Style (16th Edition):
Narkar, Ameya R. “REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY.” 2018. Doctoral Dissertation, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etdr/741.
MLA Handbook (7th Edition):
Narkar, Ameya R. “REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY.” 2018. Web. 24 Jan 2021.
Vancouver:
Narkar AR. REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY. [Internet] [Doctoral dissertation]. Michigan Technological University; 2018. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etdr/741.
Council of Science Editors:
Narkar AR. REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY. [Doctoral Dissertation]. Michigan Technological University; 2018. Available from: https://digitalcommons.mtu.edu/etdr/741

Michigan Technological University
5.
Narkar, Ameya Ravindra.
PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION.
Degree: PhD, Department of Biomedical Engineering, 2015, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etds/1002
► Smart hydrogel adhesives with tunable properties consist of adhesive moieties in the polymer network that respond to external stimuli like pH, temperature, etc. Responsiveness…
(more)
▼ Smart hydrogel adhesives with tunable properties consist of adhesive moieties in the polymer network that respond to external stimuli like pH, temperature, etc. Responsiveness of smart adhesives to pH, in particular, is important because of the simple actuation mechanism and the ability to achieve facile bonding and debonding upon command. Covalently crosslinked hydrogel adhesives were prepared by employing an N-HEAA (hydroxyethyl acrylamide) backbone embedded with dopamine methacrylamide (DMA), a marine mussel inspired adhesive protein and 3-acrylamido phenylboronic acid (AAPBA), to determine the effect of pH on the interfacial binding properties of the hydrogel adhesive with a borosilicate glass substrate. Swelling tests were performed to determine the response of the synthesized hydrogels to changes in pH values. These tests revealed that in a pH 3 buffered solution, hydrogels containing DMA and AAPBA showed a shrinking trend, while at pH 9, a swelling phenomenon was observed. The evidence from oscillatory rheometry tests exhibited elevated loss moduli (Gʹ) for hydrogels with DMA and AAPBA at pH 9, when compared to the relevant controls. In conjunction, the data from swelling tests and rheometry explained the unusual swelling of the hydrogels and formation of the catechol-boronate complex at pH 9, which caused more than an order of magnitude of increase in the Gʺ owing to the viscous dissipation of energy at that pH as compared to the control gels. The interfacial binding properties were tested by performing contact mechanics tests, in the presence of an acidic/basic medium. The maximum work of adhesion values of 0.59<em>mJ/m
2</em> were obtained for hydrogels with 2.5mol% DMA and 10mol%AAPBA in the polymer network, when tested against a borosilicate glass surface wetted with 250
μL of the pH 3 solution. At pH 9, this value reduced to as much as 1
/5th of its value at pH 3. Earlier works have proposed that the oxidation of the catecholic groups that are chiefly responsible for adhesion with an inorganic substrate, is a deterrent to the adhesive properties of a hydrogel. We have accomplished the development of a model adhesive system in which we utilized the pH responsiveness of the hydrogels to demonstrate the elevated and reduced works of adhesion at acidic and basic pHs respectively. We believe that the catechol- boronic acid complex at pH 9 will allow for the reversible DOPA- facilitated adhesion. Reversibility studies performed in this direction revealed that while the hydrogels could recover their shape in terms of the measured diameters, further testing and analysis is required for understanding the ideal composition of the hydrogel and environmental trigger to actuate reversibility.
Advisors/Committee Members: Bruce P. Lee.
Subjects/Keywords: adhesive; catechol; compelxation; pH; reversible; Biomedical Engineering and Bioengineering
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Narkar, A. R. (2015). PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etds/1002
Chicago Manual of Style (16th Edition):
Narkar, Ameya Ravindra. “PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION.” 2015. Doctoral Dissertation, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etds/1002.
MLA Handbook (7th Edition):
Narkar, Ameya Ravindra. “PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION.” 2015. Web. 24 Jan 2021.
Vancouver:
Narkar AR. PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION. [Internet] [Doctoral dissertation]. Michigan Technological University; 2015. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etds/1002.
Council of Science Editors:
Narkar AR. PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION. [Doctoral Dissertation]. Michigan Technological University; 2015. Available from: https://digitalcommons.mtu.edu/etds/1002

Michigan Technological University
6.
Pinnaratip, Rattapol.
EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL.
Degree: MS, Department of Biomedical Engineering, 2017, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/534
► Cell infiltration is one of the most important characteristics of a degradable and bioactive biomaterial. Poly (ethylene glycol) (PEG), as one of the most…
(more)
▼ Cell infiltration is one of the most important characteristics of a degradable and bioactive biomaterial. Poly (ethylene glycol) (PEG), as one of the most commonly used polymer in medicine, also faces such problems. This issue also prevalence in PEG derivatives such as poly (ethylene glycol)-glutaric acid-dopamine (PEG-GA-DM) which is a versatile bioadhesive. As a promising bioadhesive system that can be used and modified to suit various type of applications, modification to overcome cellular infiltration and degradation issues will help further expand the usefulness of the material in tissue repair application. The degradation issue is already preliminary solved by introducing ester linkage through carboxylic acid functionalization via glutaric acid incorporation. However, the resulting degradation rate is still not ideal. To further modify the porosity, pore structure, and degradation rate of the polymer, various types of non-crystalline silica particles were introduced into the polymer network. The benefit of including silica particles into the gel system may not limited only to the degradation and pore structure. One of the most interesting effects that this ceramic material may contain is its ability to release silica byproduct, specifically hydrolyzed silica or orthosilicic acid. Orthosilicic acid was proved to positively affect collagenous extracellular matrix formation as well as improves keratin and collagen based organ’s health. Using various formulations, a number of PEG-GA-DA and silica composite improvements were investigated. The silica’s contributions to a number of changes include shortened gelation time, increased mechanical strength, faster initial degradation, higher structural stability after degradation, and, ultimately, change in porous structure. It is interesting that PEG-GA-DA silica composite not only provided improvement for the PEG network but also provide benefit for innate property of silica particle. With silicic acid as degraded product, the composite also facilitates extracellular matrix formation as well as therapeutic macrophages recruitment.
Advisors/Committee Members: Rupak M. Rajachar, Bruce P. Lee.
Subjects/Keywords: Silica particle; PEG; adhesive; bioactive material; poly(ethylene glycol); Biomaterials; Molecular, Cellular, and Tissue Engineering; Other Biomedical Engineering and Bioengineering; Polymer Science
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pinnaratip, R. (2017). EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL. (Masters Thesis). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/534
Chicago Manual of Style (16th Edition):
Pinnaratip, Rattapol. “EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL.” 2017. Masters Thesis, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etdr/534.
MLA Handbook (7th Edition):
Pinnaratip, Rattapol. “EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL.” 2017. Web. 24 Jan 2021.
Vancouver:
Pinnaratip R. EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL. [Internet] [Masters thesis]. Michigan Technological University; 2017. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etdr/534.
Council of Science Editors:
Pinnaratip R. EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL. [Masters Thesis]. Michigan Technological University; 2017. Available from: https://digitalcommons.mtu.edu/etdr/534

Michigan Technological University
7.
Pinnaratip, Rattapol.
STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES.
Degree: PhD, Department of Biomedical Engineering, 2020, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/1044
► Mussel adhesive proteins contain catechol moiety, which allows the protein to crosslinked, solidify, and adhere to surrounding surfaces even under wet conditions. Incorporating the…
(more)
▼ Mussel adhesive proteins contain catechol moiety, which allows the protein to crosslinked, solidify, and adhere to surrounding surfaces even under wet conditions. Incorporating the catechol moiety into polymeric adhesive resulted in bioadhesive, which still functions under wet conditions. However, the adhesive must be oxidized in order to crosslink and adhere to surfaces. The oxidation process of catechol adhesive was proven to produce singlet oxygen, superoxide, and hydrogen peroxide (H
2O
2). These reactive oxygen species, with lack of control, can wreak havoc to the biological system causing poor healing and undesired biological response.
Here, we studied the silica particle system as a means to control H
2O
2 concentration. After an exploratory investigation on silica particle synthesis and modification, acid-treated silica particle (AHSi) was developed with a highly hydrophilic surface. We then explore the use of reinforcement phase incorporation, creating particle-adhesive composites and studied the effect which the particle incorporation imparts into the adhesives. The model adhesive is polyethylene glycol functionalized with glutaric acid and dopamine, creating a biodegradable adhesive hydrogel. We demonstrated that incorporation of a model particle, silica particle, into a catechol adhesive resulting in a mechanically stronger adhesive with an increase in stiffness, adhesion strength, and structural integrity even after partially degraded. Moreover, the composite adhesive was gelled faster and degrade slower than native PEG-DA adhesive. The composite also demonstrated a reduction in the concentration of H
2O
2. The particle not only reduces H
2O
2 but also found to be releasing soluble silica in a biologically relevant concentration further improve their bioreactivity. The silica nanoparticle incorporated catechol-based composite demonstrated a reduction in cytotoxicity on rat dermal fibroblast, human keratinocyte, and human tenocyte, three types of cells that react differently to elevated oxidative stress.
Interestingly, all cell types have demonstrated an increase in cell proliferation, raising the possibility of developing the composite adhesive further. The last part of the study involved a prediction model that helps narrow down the formulation to be tested in vivo. Full-thickness dermal wound model in mice was utilized to study the predicted formulation. The results from the animal model suggested that PEG adhesive alone can alter the biological response with accelerated wound healing. However, the incorporation of AHSi proved to successfully bridge the gap between accelerated wound healing and better wound remodeling. This dissertation describes various strategies used to tune the H
2O
2 concentration released from catechol adhesives to tune its biological response which involved silica particle modification with minimal change in chemical composition, and the selection of adhesive…
Advisors/Committee Members: Bruce P. Lee, Rupak M. Rajachar.
Subjects/Keywords: Silica Particle; Wound Healing; Controlled Release; Catechol Adhesive; Hydrogen Peroxide; Composite Adhesive; Biomaterials
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pinnaratip, R. (2020). STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/1044
Chicago Manual of Style (16th Edition):
Pinnaratip, Rattapol. “STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES.” 2020. Doctoral Dissertation, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etdr/1044.
MLA Handbook (7th Edition):
Pinnaratip, Rattapol. “STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES.” 2020. Web. 24 Jan 2021.
Vancouver:
Pinnaratip R. STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES. [Internet] [Doctoral dissertation]. Michigan Technological University; 2020. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etdr/1044.
Council of Science Editors:
Pinnaratip R. STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES. [Doctoral Dissertation]. Michigan Technological University; 2020. Available from: https://digitalcommons.mtu.edu/etdr/1044

Michigan Technological University
8.
Zhang, Zhongtian.
Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors.
Degree: MS, Department of Biomedical Engineering, 2018, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/701
► In this research, the degradable polymer 4-arm poly (ethylene glycol)-glutaric acid-dopamine (PEG-GA-DM4) was synthesized. The degradation behavior of crosslinked PEG-GA-DM4 bioadhesive was studied with…
(more)
▼ In this research, the degradable polymer 4-arm poly (ethylene glycol)-glutaric acid-dopamine (PEG-GA-DM
4) was synthesized. The degradation behavior of crosslinked PEG-GA-DM
4 bioadhesive was studied with mass tracking, oscillatory rheology, and magnetoelastic (ME) sensors. Changes in mechanical properties were correlated with both dry mass and wet mass changes during the degradation. The results indicate that the loss of mechanical property in the bioadhesive can take place without losing the dry mass. The mass loss profile cannot describe the degradation behavior completely. In addition to studying the degradation of PEG-GA-DM
4, this research also confirms the application of ME sensors as a means to study the mechanical and degradation behavior of bioadhesive.
Advisors/Committee Members: Bruce P. Lee, Keat Ghee Ong.
Subjects/Keywords: tissue adhesive; degradation; mechanical property; magnetoelastic; rheology; Biology and Biomimetic Materials; Biomaterials; Biomedical Devices and Instrumentation
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhang, Z. (2018). Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors. (Masters Thesis). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/701
Chicago Manual of Style (16th Edition):
Zhang, Zhongtian. “Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors.” 2018. Masters Thesis, Michigan Technological University. Accessed January 24, 2021.
https://digitalcommons.mtu.edu/etdr/701.
MLA Handbook (7th Edition):
Zhang, Zhongtian. “Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors.” 2018. Web. 24 Jan 2021.
Vancouver:
Zhang Z. Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors. [Internet] [Masters thesis]. Michigan Technological University; 2018. [cited 2021 Jan 24].
Available from: https://digitalcommons.mtu.edu/etdr/701.
Council of Science Editors:
Zhang Z. Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors. [Masters Thesis]. Michigan Technological University; 2018. Available from: https://digitalcommons.mtu.edu/etdr/701
.