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You searched for +publisher:"Governors State University" +contributor:("Shailendra Kumar, Ph.D."). Showing records 1 – 3 of 3 total matches.

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1. Kanduri, Gouthami. Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique.

Degree: MS, Analytical Chemistry, 2012, Governors State University

The pharmaceutical industry mainly uses chromatographic techniques such as High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), to determine the quantity of the active ingredient and other material in the drugs. A large effort goes into developing methods using chromatography techniques. The method development and running HPLC and GC are time consuming. Methods need to be updated as the chromatographic columns and the instruments wear out. On the other hand, Nuclear Magnetic Resonance (NMR) technique is mainly used for qualitative analysis to determine the identity of compounds. However, Proton-NMR technique does provide quantitative information of compounds but to develop a method an internal standard of known concentration is required. We are using q-NMR (quantitative NMR) to quantitatively analyze acetylsalicylic acid and acetaminophen by Proton-NMR using diethyl ether as an internal standard. The area of NMR signals shows a linear relationship with the concentration. Acetylsalicylic acid and acetaminophen were chosen as a representative drug. The method is useful for a wide variety of drugs. Our future studies include using a variety of appropriate internal standards to determine concentrations of several organic compounds including various pharmaceutical drugs and petroleum products. Advisors/Committee Members: Shailendra Kumar, Ph.D., Walter Henne, Jr., Ph.D., Karen D'Arcy, Ph.D..

Subjects/Keywords: Analytical Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Kanduri, G. (2012). Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique. (Thesis). Governors State University. Retrieved from http://opus.govst.edu/theses/10

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Kanduri, Gouthami. “Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique.” 2012. Thesis, Governors State University. Accessed February 20, 2019. http://opus.govst.edu/theses/10.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Kanduri, Gouthami. “Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique.” 2012. Web. 20 Feb 2019.

Vancouver:

Kanduri G. Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique. [Internet] [Thesis]. Governors State University; 2012. [cited 2019 Feb 20]. Available from: http://opus.govst.edu/theses/10.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kanduri G. Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique. [Thesis]. Governors State University; 2012. Available from: http://opus.govst.edu/theses/10

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Jakkampudi, Maneesha. Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector.

Degree: MS, Analytical Chemistry, 2012, Governors State University

Photo oxidation of proteins leads to the formation of cross links of the amino acids in the proteins and leads to induction of cataracts, photo aging of the skin and in the Photodynamic therapy of tumors. Oxidation of proteins involves significant modification of particular amino acids residues with consequent photodynamic damage. Studies show that tryptophan, tyrosine and histidine side chains gives rise to semi stable peroxides on exposure to singlet oxygen these oxidized structures are studied with low temperature NMR. These peroxides or species derived from them have been postulated as key intermediates in subsequent reactions such as cross linking of oxidized amino acids with unmodified residues. It is difficult to identify modified amino acids in photo dynamically treated proteins and to determine their positions/locations .It is tedious to isolate the cross linked amino acid moieties from proteins, hence low molecular weight histidine and imidazole derivatives have been used to study the detailed reaction mechanisms. photo oxidation of N acetyl histidine ethyl amide derivative of histidine in which the amine group and the carboxylic acid group have been converted to amide bonds .this conversion makes this amino acid a peptide resembling amino acid so that its photo oxidation mimics the photo oxidation of histidine moiety in proteins. Use of N-Benzoyl-L histidine as a model compound elucidates the chemical structure and mechanisms of the formation of His-His cross links .Benzoyl group protects the alpha amino group of histidine participation in the cross link reactions .Benzoyl absorbs strongly in UV hence permits the spectroscopic identification Rose Bengal is used as a photo sensitizer which sensitizes primarily by the singlet oxygen mechanism in the aqueous solution It causes extensive damage to the imidazole ring of Histidine by forming semi stable peroxides which results in the formation of Hydrated Imidazolone with the second molecule of N acetyl histidine ethyl amide. The formation of this dimer shows the strong possibility of cross linking in the histidine residues in proteins. Lab synthesized N acetyl L histidine ethyl amide was analyzed by TLC, Melting point and NMR and declared that it was impure. Purification process was done through re-crystallization and Running the analyte down through silica column by gradient elution using three different solvents Hexanes, Ethyl acetate and Methanol. Synthesis of N acetyl L histidine ethyl amide meant to synthesize in lab Lab synthesis is done in two steps: Step1. Synthesizing N acetyl L histidine ethyl ester from L Histidine Step2. Synthesizing N acetyl L Histidine ethyl amide from N acetyl L histidine ethyl ester N acetyl L histidine ethyl ester is synthesized in lab on proceeding upon second step reconversion of N acetyl L histidine ethyl ester to L histidine was observed. Advisors/Committee Members: Shailendra Kumar, Ph.D., Walter Henne, Jr., Ph.D., Karen D'Arcy, Ph.D..

Subjects/Keywords: Analytical Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Jakkampudi, M. (2012). Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector. (Thesis). Governors State University. Retrieved from http://opus.govst.edu/theses/2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Jakkampudi, Maneesha. “Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector.” 2012. Thesis, Governors State University. Accessed February 20, 2019. http://opus.govst.edu/theses/2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Jakkampudi, Maneesha. “Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector.” 2012. Web. 20 Feb 2019.

Vancouver:

Jakkampudi M. Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector. [Internet] [Thesis]. Governors State University; 2012. [cited 2019 Feb 20]. Available from: http://opus.govst.edu/theses/2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jakkampudi M. Project Synthesis of N Acetyl L Histidine Ethyl Amide and Standard Operating Procedure for Fractional Collector. [Thesis]. Governors State University; 2012. Available from: http://opus.govst.edu/theses/2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Flemming, Charles, Jr. Quantitative Analysis of Acetylsalicylic Acid by q-NMR (Quantitative-Nuclear Magnetic Resonance Spectroscopy).

Degree: MS, Analytical Chemistry, 2016, Governors State University

Quantitative analysis is an important step during pharmaceutical drug development. Currently, the pharmaceutical industry uses high performance liquid chromatography (HPLC) for quantitative analysis of organic compounds as the preferred technique. The proton nuclear magnetic resonance (NMR) technique has an inherent ability to quantify a given analyte and may be used to quantify organic compounds. Quantitative nuclear magnetic resonance spectroscopy (q-NMR) of acetylsalicylic acid (ASA) was conducted using tert-butyl alcohol (tert-butanol) as the internal standard and deuterated chloroform (CDCl3) as the solvent. Preparations of ASA solutions in low and high concentration ranges, 1.0 mM – 10.1 mM and 10.0 mM – 100.1 mM respectively, were analyzed to determine a linear correlation between the concentrations of ASA with the intensities of methyl peaks and aromatic ring proton peaks using Topspin software. The q-NMR analysis of high concentration ASA solutions showed a strong linear correlation (R2 values > 0.9994) and high precision (% RSD values < 1%) for both the average methyl peak areas and the average aromatic ring proton peak areas. However, the low concentration ASA solutions showed a weak linear correlation (R2 values > 0.9914) but, fairly good precision (% RSD values < 5%) for both the average methyl peak areas and the average aromatic ring proton peak areas. Therefore, the q-NMR technique is a viable alternative for quantitative analysis of high concentration ASA methyl peak areas and aromatic ring proton peak areas, but it is not suitable for the low concentration ASA solutions. The low concentration (1.0 mM – 10.1 mM) ASA solutions were also analyzed by the conventional HPLC method; the results obtained were compared with the results from the q-NMR technique. A comparison of q-NMR data to HPLC data focused on linearity, precision, and acquisition time for the acetylsalicylic acid solutions at low concentrations. The HPLC data showed a strong linear correlation with an R2 value of 0.9997 and high precision with a % RSD < 0.9% for the average ASA peak areas. A comparison of q-NMR data to HPLC data for the low concentration ASA solutions showed that the q-NMR technique used less solvents and less time for data acquisition. Advisors/Committee Members: Shailendra Kumar, Ph.D., Joong-Won Shin, Ph.D., Walter Henne, Jr., Ph.D..

Subjects/Keywords: Proton nuclear magnetic resonance; spectroscopy; q-NMR; chromatography; HPLC; Analytical Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Flemming, Charles, J. (2016). Quantitative Analysis of Acetylsalicylic Acid by q-NMR (Quantitative-Nuclear Magnetic Resonance Spectroscopy). (Thesis). Governors State University. Retrieved from http://opus.govst.edu/theses/89

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Flemming, Charles, Jr. “Quantitative Analysis of Acetylsalicylic Acid by q-NMR (Quantitative-Nuclear Magnetic Resonance Spectroscopy).” 2016. Thesis, Governors State University. Accessed February 20, 2019. http://opus.govst.edu/theses/89.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Flemming, Charles, Jr. “Quantitative Analysis of Acetylsalicylic Acid by q-NMR (Quantitative-Nuclear Magnetic Resonance Spectroscopy).” 2016. Web. 20 Feb 2019.

Vancouver:

Flemming, Charles J. Quantitative Analysis of Acetylsalicylic Acid by q-NMR (Quantitative-Nuclear Magnetic Resonance Spectroscopy). [Internet] [Thesis]. Governors State University; 2016. [cited 2019 Feb 20]. Available from: http://opus.govst.edu/theses/89.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Flemming, Charles J. Quantitative Analysis of Acetylsalicylic Acid by q-NMR (Quantitative-Nuclear Magnetic Resonance Spectroscopy). [Thesis]. Governors State University; 2016. Available from: http://opus.govst.edu/theses/89

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.