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You searched for +publisher:"Georgia Tech" +contributor:("Udaykumar, H.S."). One record found.

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Georgia Tech

1. Carnevale, Kevin A. Finite-Difference Model of Cell Dehydration During Cryopreservation.

Degree: MS, Mechanical Engineering, 2004, Georgia Tech

A numerical model for describing the kinetics of intracellular water transport during cryopreservation was developed. As ice is formed outside the cell, depleting the extracellular liquid of water, the cell will experience an osmotic pressure difference across its membrane, which causes cell dehydration and concomitant shrinkage. Although Mazur (1963) has previously modeled this phenomenon as a two-compartment system with membrane limited transport, the assumption of well-mixed compartments breaks down at large Biot numbers. Therefore, we have developed a numerical solution to this moving-boundary problem, including diffusive transport in the intracellular liquid, in addition to the osmotically driven membrane flux. Our model uses a modified Crank-Nicolson scheme with a non-uniform Eulerian-Lagrangian grid, and is able to reproduce predictions from Mazurs model at low Biot numbers, while generating novel predictions at high Biot numbers. Given that cell damage may result from excessive water loss, our model can be used to predict freezing methods that minimize the probability of cell injury during the cryopreservation process. Advisors/Committee Members: Karlsson, Jens (Committee Chair), Udaykumar, H.S. (Committee Member), Wick, Timothy (Committee Member).

Subjects/Keywords: Cryobiology; Cryopreservation; Membrane permeability; Vitrification; Membrane-limited water transport; Diffusion-limited water transport; Crank-Nicolson; Biot number; Peclet number; Dimensional analysis; Cell dehydration; Mathematical models; Finite differences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Carnevale, K. A. (2004). Finite-Difference Model of Cell Dehydration During Cryopreservation. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/7258

Chicago Manual of Style (16th Edition):

Carnevale, Kevin A. “Finite-Difference Model of Cell Dehydration During Cryopreservation.” 2004. Masters Thesis, Georgia Tech. Accessed April 11, 2021. http://hdl.handle.net/1853/7258.

MLA Handbook (7th Edition):

Carnevale, Kevin A. “Finite-Difference Model of Cell Dehydration During Cryopreservation.” 2004. Web. 11 Apr 2021.

Vancouver:

Carnevale KA. Finite-Difference Model of Cell Dehydration During Cryopreservation. [Internet] [Masters thesis]. Georgia Tech; 2004. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1853/7258.

Council of Science Editors:

Carnevale KA. Finite-Difference Model of Cell Dehydration During Cryopreservation. [Masters Thesis]. Georgia Tech; 2004. Available from: http://hdl.handle.net/1853/7258

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