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Georgia Tech
1.
Williams, Chrysanthi.
Perfusion bioreactor for tissue-engineered blood vessels.
Degree: PhD, Bioengineering, 2003, Georgia Tech
URL: http://hdl.handle.net/1853/9455 
Subjects/Keywords: Tissue engineering; Bioreactors Design and construction; Vascular grafts; Vascular grafts; Tissue engineering; Bioreactors Design and construction
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APA (6th Edition):
Williams, C. (2003). Perfusion bioreactor for tissue-engineered blood vessels. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/9455
Chicago Manual of Style (16th Edition):
Williams, Chrysanthi. “Perfusion bioreactor for tissue-engineered blood vessels.” 2003. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021.
http://hdl.handle.net/1853/9455.
MLA Handbook (7th Edition):
Williams, Chrysanthi. “Perfusion bioreactor for tissue-engineered blood vessels.” 2003. Web. 06 Mar 2021.
Vancouver:
Williams C. Perfusion bioreactor for tissue-engineered blood vessels. [Internet] [Doctoral dissertation]. Georgia Tech; 2003. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1853/9455.
Council of Science Editors:
Williams C. Perfusion bioreactor for tissue-engineered blood vessels. [Doctoral Dissertation]. Georgia Tech; 2003. Available from: http://hdl.handle.net/1853/9455
Georgia Tech
2.
Konduri, Suchitra.
The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System.
Degree: MS, Chemical Engineering, 2005, Georgia Tech
URL: http://hdl.handle.net/1853/6999
► The aortic valve functions in a complex mechanical environment which leads to force dependent cellular and tissue responses. Characterization of these responses provides a fundamental…
(more)
▼ The aortic valve functions in a complex mechanical environment which leads to force dependent cellular and tissue responses. Characterization of these responses provides a fundamental understanding of valve pathogenesis. The aim of this work was to develop an ex vivo organ culture system capable of simulating physiological aortic pressures and flow rates, and study the biological characteristics of native porcine aortic valves cultured in the system. Collagen, sGAG and elastin content of the valve leaflets were measured and cusp morphology, cell phenotype, cell proliferation and apoptosis were examined. Presence of endothelial cells (ECs) on the leaflet surface was also evaluated. The differences in collagen, sGAG and elastin contents were not significant (p greater than0.05) between the cultured and fresh valve leaflets. The cultured valves maintained the structural integrity of the leaflets while preserving the native morphology and cell phenotype. Cell phenotype in leaflets incubated statically under atmospheric conditions decreased compared to fresh and cultured valve leaflets, indicating the importance of mechanical forces in maintaining the natural biology of the valve leaflets. ECs were retained on the surfaces of cultured leaflets with no remodeling of the leaflets. The number of apoptotic cells in the cultured leaflets was significantly (p less than 0.05) less than in the statically incubated leaflets and comparable to fresh leaflets. The sterile ex vivo organ culture system thus maintained the viability and native biological characteristics of the aortic valves that were cultured under dynamic conditions for a period of 48 hours.
Advisors/Committee Members: Dr. Ajit P.Yoganathan (Committee Chair), Dr. Athanassios Sambanis (Committee Member), Dr. Timothy M. Wick (Committee Member).
Subjects/Keywords: Flow and pressure waveforms; Porcine aortic valve leaflets; Extracellular matrix components; Cell phenotype; Endothelial cells; Organ culture system; Tissue morphology
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APA (6th Edition):
Konduri, S. (2005). The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/6999
Chicago Manual of Style (16th Edition):
Konduri, Suchitra. “The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System.” 2005. Masters Thesis, Georgia Tech. Accessed March 06, 2021.
http://hdl.handle.net/1853/6999.
MLA Handbook (7th Edition):
Konduri, Suchitra. “The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System.” 2005. Web. 06 Mar 2021.
Vancouver:
Konduri S. The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System. [Internet] [Masters thesis]. Georgia Tech; 2005. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1853/6999.
Council of Science Editors:
Konduri S. The Influence of Normal Physiological Forces on Porcine Aortic Heart Valves in a Sterile Ex Vivo Pulsatile Organ Culture System. [Masters Thesis]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/6999
Georgia Tech
3.
Bartling, Karsten.
Apoferritin Crystallization in relation to Eye Cataract.
Degree: PhD, Bioengineering, 2006, Georgia Tech
URL: http://hdl.handle.net/1853/14111
► Protein crystallization is significant in both biotechnology and biomedical applications. In biotechnology, crystallization is essential for determining the structure of both native and synthesized therapeutically…
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▼ Protein crystallization is significant in both biotechnology and biomedical applications. In biotechnology, crystallization is essential for determining the structure of both native and synthesized therapeutically important proteins. It can also be used as a final purification step and as a stable form for protein storage. With regard to biomedical systems, protein crystallization appears to be involved in the development and manifestation of certain human diseases. In particular, there exists evidence that L-rich ferritin crystals are involved in Hereditary Hyperferritinemia Cataract Syndrome (HHCS).
In the current research a microbatch crystallization apparatus has been introduced that enables (1) multiple batch crystallization experiments at various temperatures and solution conditions in parallel and (2) quantitative monitoring of crystal growth without disturbing the progress of an experiment for observation. The primary application of the apparatus is, but not limited to, screening of protein crystallization conditions, although the system can also be used for other macromolecular and small-molecule crystallization experiments.
Multiwell microbatch experiments demonstrated the dependence of apoferritin crystal growth kinetics and final crystal size on temperature and cadmium concentration. Although the solubility of apoferritin might be independent of temperature, the results of this study show that the crystal growth kinetics are affected by temperature, profoundly under some conditions.
For apoferritin under near physiological conditions the solution thermodynamics in the form of the second virial coefficient have proofed to be a valuable predictor for the crystallization outcome. Furthermore, the significance of the elevated level of some divalent cations in cataractous lenses has been studied both in dilute solutions and under crystallization conditions and cadmium seems to be sole menace in apoferritin condensation.
Advisors/Committee Members: Ronald W. Rousseau (Committee Chair), Athanassios Sambanis (Committee Co-Chair), Joe LeDoux (Committee Member), Timothy M. Wick (Committee Member), Victor Breedveld (Committee Member).
Subjects/Keywords: Activation energy for crystallization; Cataract; Apoferritin; Second virial coefficient; Crystallization; Thermal gradient; Light Scattering; Microbatch
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APA (6th Edition):
Bartling, K. (2006). Apoferritin Crystallization in relation to Eye Cataract. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/14111
Chicago Manual of Style (16th Edition):
Bartling, Karsten. “Apoferritin Crystallization in relation to Eye Cataract.” 2006. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021.
http://hdl.handle.net/1853/14111.
MLA Handbook (7th Edition):
Bartling, Karsten. “Apoferritin Crystallization in relation to Eye Cataract.” 2006. Web. 06 Mar 2021.
Vancouver:
Bartling K. Apoferritin Crystallization in relation to Eye Cataract. [Internet] [Doctoral dissertation]. Georgia Tech; 2006. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1853/14111.
Council of Science Editors:
Bartling K. Apoferritin Crystallization in relation to Eye Cataract. [Doctoral Dissertation]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/14111
Georgia Tech
4.
Fallon, Anna Marie.
The Development of a Novel in vitro Flow System to Evaluate Platelet Activation and Procoagulant Potential Induced by Bileaflet Mechanical Heart Valve Leakage Jets.
Degree: PhD, Chemical Engineering, 2006, Georgia Tech
URL: http://hdl.handle.net/1853/10451
► Bileaflet mechanical heart valves (BMHVs) are prone to thrombus formation in the hinge region due to high shear stress combined with stagnation regions. This thesis…
(more)
▼ Bileaflet mechanical heart valves (BMHVs) are prone to thrombus formation in the hinge region due to high shear stress combined with stagnation regions. This thesis research addresses the hypothesis that models that isolate and mimic BMHV hinge geometries can be used to quantitatively characterize procoagulant potential using a novel in vitro blood flow system. Furthermore, these results can be correlated with digital particle image velocimetry (DPIV) measurements detailing flow fields for the same models.
The significant findings were that: 1) recalcification of recirculating citrated blood markedly increases the magnitude of thrombus forming reactions and the sensitivity for their detection; 2) platelet activation, and the presence of adequate platelet numbers are essential for the activation of coagulation under conditions of high shear; and 3) thrombin formation can be inhibited by blocking the platelet receptors that facilitate platelet aggregation.
The DPIV studies give some insight into why different channel geometries resulted in varying propensities for coagulation. The channel geometries with abrupt changes in diameter induced significantly higher levels of TAT and also formed jets that were subject to increased entrainment of the stagnant fluid in the chamber. This entrainment enables more mixing of the shear-activated platelets with the surrounding flow, which can propagate the coagulation cascade, thus increasing the chance for thrombus formation.
The influence of abrupt changes in diameter was also evident in the BMHV human blood studies. The MP valve, which has a tortuous hinge pathway, induced significantly more TAT formation than the SJM Standard valve with a smoother hinge channel. Thus, BMHV hinge geometry should be as smooth and free of diameter changes as possible to eliminate stagnation regions that enable activated platelets to congregate and propagate the coagulation cascade.
Leakage gap width also had a significant effect not only on procoagulant potential but also on platelet activation. Both the low and high leaker prototype valves had significantly higher levels of platelet activation compared to the SJM Standard valve, but only the low leaker valve demonstrated a higher propensity for coagulation. Thus, to minimize both platelet activation and thromboemboli formation, an optimal gap width should be maintained for BMHVs.
Advisors/Committee Members: Ajit P. Yoganathan (Committee Chair), Dale E. Edmondson (Committee Member), Peter J. Ludovice (Committee Member), Stephen R. Hanson (Committee Member), Timothy M. Wick (Committee Member).
Subjects/Keywords: Blood; Thrombosis; Mechanical heart valves; Platelets; Shear stress
Record Details
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APA ·
Chicago ·
MLA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fallon, A. M. (2006). The Development of a Novel in vitro Flow System to Evaluate Platelet Activation and Procoagulant Potential Induced by Bileaflet Mechanical Heart Valve Leakage Jets. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/10451
Chicago Manual of Style (16th Edition):
Fallon, Anna Marie. “The Development of a Novel in vitro Flow System to Evaluate Platelet Activation and Procoagulant Potential Induced by Bileaflet Mechanical Heart Valve Leakage Jets.” 2006. Doctoral Dissertation, Georgia Tech. Accessed March 06, 2021.
http://hdl.handle.net/1853/10451.
MLA Handbook (7th Edition):
Fallon, Anna Marie. “The Development of a Novel in vitro Flow System to Evaluate Platelet Activation and Procoagulant Potential Induced by Bileaflet Mechanical Heart Valve Leakage Jets.” 2006. Web. 06 Mar 2021.
Vancouver:
Fallon AM. The Development of a Novel in vitro Flow System to Evaluate Platelet Activation and Procoagulant Potential Induced by Bileaflet Mechanical Heart Valve Leakage Jets. [Internet] [Doctoral dissertation]. Georgia Tech; 2006. [cited 2021 Mar 06].
Available from: http://hdl.handle.net/1853/10451.
Council of Science Editors:
Fallon AM. The Development of a Novel in vitro Flow System to Evaluate Platelet Activation and Procoagulant Potential Induced by Bileaflet Mechanical Heart Valve Leakage Jets. [Doctoral Dissertation]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/10451
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