+publisher:"Georgia Tech" +contributor:("Taite, Lakeshia J.")

Georgia Tech

1. Liu, Wenying. Electrospun nanofibers for regenerative medicine.

Degree: PhD, Chemical and Biomolecular Engineering, 2014, Georgia Tech

► Electrospun nanofibers represent a class of versatile scaffolds for tissue engineering applications owing to their ability to mimic the nanoscale features of the native extracellular… (more)

▼ Electrospun nanofibers represent a class of versatile scaffolds for tissue engineering applications owing to their ability to mimic the nanoscale features of the native extracellular matrix (ECM). In addition, nanofibers produced by electrospinning can be readily collected as uniaxially aligned assemblies to recapitulate the architecture of the ECM in tissues with anisotropic characteristics, such as tendon-to-bone insertions, tendons, and nerves. This dissertation focuses on the design, fabrication, functionalization, and assessment of various types of scaffolds consisting of aligned nanofibers, which can be used to augment regeneration in tissues with anisotropic structures. Briefly, for tendon-to-bone insertion repair, I assessed the capability of aligned nanofibers with a gradient in mineral content to induce spatially graded osteogenesis of adipose-derived mesenchymal stem cells (ASCs). I also developed an alternative approach to the production of a gradient in the density of osteoblasts. The graded pattern of osteoblasts generated using both approaches could mimic their distribution in the native tendon-to-bone insertion. To further enhance the stiffness of the scaffolds, a new solution was developed to coat the scaffold with a thicker mineral layer. In a third project, a novel method of generating crimp in aligned nanofibers was developed. A solvent plasticizer was employed to release the residual stress retained in the nanofibers during electrospinning, which led to the generation of crimp. Finally, the outgrowth of neurites derived from embryoid bodies (EBs) was studied using aligned nanofibers as the substrates. Depending on the strength of adhesion between nanofibers and neurites, two patterns of outgrowth – parallel and perpendicular (to the alignment) – were observed. Maturation of neurons derived from dissociated EBs was also investigated, as characterized by their extracellular action potential and the ability to form neuromuscular junctions with co-cultured muscle cells. Advisors/Committee Members: Xia, Younan (advisor), Behrens, Sven H. (committee member), Champion, Julie A. (committee member), García, Andrés J. (committee member), Taite, Lakeshia J. (committee member).

Subjects/Keywords: Electrospinning; Nanofibers

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APA (6^th Edition):

Liu, W. (2014). Electrospun nanofibers for regenerative medicine. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/54305

Chicago Manual of Style (16^th Edition):

Liu, Wenying. “Electrospun nanofibers for regenerative medicine.” 2014. Doctoral Dissertation, Georgia Tech. Accessed April 17, 2021. http://hdl.handle.net/1853/54305.

MLA Handbook (7^th Edition):

Liu, Wenying. “Electrospun nanofibers for regenerative medicine.” 2014. Web. 17 Apr 2021.

Vancouver:

Liu W. Electrospun nanofibers for regenerative medicine. [Internet] [Doctoral dissertation]. Georgia Tech; 2014. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1853/54305.

Council of Science Editors:

Liu W. Electrospun nanofibers for regenerative medicine. [Doctoral Dissertation]. Georgia Tech; 2014. Available from: http://hdl.handle.net/1853/54305

2. Chang, Kai. Structural modification of poly(n-isopropylacrylamide) for drug delivery applications.

Degree: PhD, Chemical and Biomolecular Engineering, 2013, Georgia Tech

URL: http://hdl.handle.net/1853/48947

► Polymeric biomaterials have become ubiquitous in modern medical devices. ‘Smart’ materials, materials that respond to external stimuli, have been of particular interest for biomedical applications… (more)

▼ Polymeric biomaterials have become ubiquitous in modern medical devices. ‘Smart’ materials, materials that respond to external stimuli, have been of particular interest for biomedical applications such as drug delivery. Poly(n-isopropylacrylamide) (pNIPAAm) is the best studied thermally responsive, biocompatible, ‘smart’ polymer and has been integrated into many potential drug delivery devices; however, the architectural design of the polymer in these devices is often overlooked. My research focus was the exploration of pNIPAAm architecture for biological applications. Two new biomaterials were synthesized as a result. Architectural modification of linear pNIPAAm was used to synthesize a well-defined homopolymer pNIPAAm with a sharp transition slightly above normal body temperature under isotonic conditions. This polymer required a combination of polymerization and control techniques including controlled radical polymerization, hydrogen bond induced tacticity, and end-group manipulation. The synthesis of this polymer opened up a variety of biomedical possibilities, one of which is the use of these polymers in a novel hydrogel system. Through the use of the controlled linear pNIPAAm synthesized through chain architectural modification, hydrogels with physiological transition temperatures were also synthesized. These hydrogels showed greater shrinking properties than traditional hydrogels synthesized in the same manner and showed physiological mechanical properties. Highly branched pNIPAAm was also optimized for biological applications. In this case, the branching reduced the efficacy of end-groups in transition temperature modification but increased the efficacy of certain copolymers. The resulting biomaterial was incorporated into a nanoparticle drug delivery system. By combining gold nanoparticles with highly branched pNIPAAm, which was designed to entrap small molecule drugs, a hybrid system was synthesized where heating of the nanoparticle through surface plasmon resonance can trigger drug release from the pNIPAAm. This system proved to be easy to synthesize, effective in loading, and controlled in release. As shown from the applications, architectural control of pNIPAAm can open up new possibilities with this polymer for biomedical applications. Small structural changes can lead to significant changes in the bulk properties of the polymer and should be considered in future pNIPAAm based medical devices. Advisors/Committee Members: Taite, Lakeshia J. (advisor), Prausnitz, Mark (committee member), Bommarius, Andreas (committee member), Champion, Julie A. (committee member), Milam, Valeria T. (committee member).

Subjects/Keywords: Polymer architecture; Thermally responsive; Polymeric drug delivery vehicle; Acrylamide; Polymeric drug delivery systems

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, K. (2013). Structural modification of poly(n-isopropylacrylamide) for drug delivery applications. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/48947

Chicago Manual of Style (16^th Edition):

Chang, Kai. “Structural modification of poly(n-isopropylacrylamide) for drug delivery applications.” 2013. Doctoral Dissertation, Georgia Tech. Accessed April 17, 2021. http://hdl.handle.net/1853/48947.

MLA Handbook (7^th Edition):

Chang, Kai. “Structural modification of poly(n-isopropylacrylamide) for drug delivery applications.” 2013. Web. 17 Apr 2021.

Vancouver:

Chang K. Structural modification of poly(n-isopropylacrylamide) for drug delivery applications. [Internet] [Doctoral dissertation]. Georgia Tech; 2013. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1853/48947.

Council of Science Editors:

Chang K. Structural modification of poly(n-isopropylacrylamide) for drug delivery applications. [Doctoral Dissertation]. Georgia Tech; 2013. Available from: http://hdl.handle.net/1853/48947

3. Morgan, M. Thomas. Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards.

Degree: PhD, Chemistry and Biochemistry, 2013, Georgia Tech

URL: http://hdl.handle.net/1853/51937

► Copper is an essential trace element for living organisms and has both known and additional suspected roles in human health and disease. The current understanding… (more)

Subjects/Keywords: CTAP-2; Sulfonyl fluoride; Sulfonate; Neopentyl; Triarylpyrazoline; Copper(I); Photoinduced electron transfer; Fluorophore; ESPT; Ligand; Aqueous; Copper in the body; Biochemistry; Fluorescent probes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morgan, M. T. (2013). Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/51937

Chicago Manual of Style (16^th Edition):

Morgan, M Thomas. “Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards.” 2013. Doctoral Dissertation, Georgia Tech. Accessed April 17, 2021. http://hdl.handle.net/1853/51937.

MLA Handbook (7^th Edition):

Morgan, M Thomas. “Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards.” 2013. Web. 17 Apr 2021.

Vancouver:

Morgan MT. Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards. [Internet] [Doctoral dissertation]. Georgia Tech; 2013. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1853/51937.

Council of Science Editors:

Morgan MT. Molecular tools for elucidating copper biochemistry: Water-soluble fluorescent probes and robust affinity standards. [Doctoral Dissertation]. Georgia Tech; 2013. Available from: http://hdl.handle.net/1853/51937

4. Krajniak, Jan. Microfluidic toolkit for scalable live imaging, developmental and lifespan dynamic studies of C. elegans with single animal resolution.

Degree: PhD, Chemical and Biomolecular Engineering, 2013, Georgia Tech

URL: http://hdl.handle.net/1853/49115

► The nematode Caenorhabditis elegans has served as one of the primary model organisms in neuroscience. As C. elegans research became more specific, so have the… (more)

▼ The nematode Caenorhabditis elegans has served as one of the primary model organisms in neuroscience. As C. elegans research became more specific, so have the biological tools for manipulating C. elegans improved and matured. Additionally, in some avenues of research, technologies have been developed to manipulate the animals in very efficient and quantitative ways. However, the field of dynamic studies has remained without significant technological support. Dynamic studies focus on processes occurring over time and span a range of time-scales of i) minutes to hours requiring continuous imaging for accurate observation, ii) hours to days requiring periodic imaging of the same animal, and iii) days to weeks requiring daily monitoring. Because of a lack of suitable tools and technologies to perform these studies, researchers have to either apply standard biological methods with limited ability to observe processes dynamically or simply cannot perform such studies with the desired set of experimental conditions. To address this problem, a comprehensive microfluidic toolkit for dynamic studies has been created. The first element is a novel method for reversible and repeatable immobilization at benign conditions in tandem with a microfluidic system for isolated culture of C. elegans with integrated temperature control. The second element is a system for efficient handling of C. elegans embryos in a high-throughput and scalable fashion for chemical and thermal embryonic stimulation with subsequent study of development. The third component is a system capable of selective immobilization of animals’ bodies, while simultaneously facilitating feeding and normal physiological function for live imaging. The last component is capable of culturing animals over their life-span with efficient animal handling, environmental control (temperature and dietary conditions), and high data content experimentation. As a whole, the work in this thesis enables dynamic studies over the whole range of time scales applicable to C. elegans research. These types of studies were previously very difficult or near impossible to perform practically. Now, instead of building population composites to understand the dynamics of a process, risking affecting physiology via the experiment itself, or dealing with extremely labor intensive physical handling of animals, a toolkit for efficient handling of C. elegans facilitating dynamic and direct observation of individual animals is available. The biological applications range from dynamically studying lipid droplet morphology or studying synaptic vesicle transport, through observing the dynamics of synaptic re-arrangement during development or the effect of cancer drugs on development, to performing high-content life-span experiments able to ascertain the relationship between aging and behavior. Additionally, many of the principles in these designs can be expanded to accommodate research on other model organisms, such as other nematode species, zebra fish embryos, or cells and embryoid bodies. Advisors/Committee Members: Lu, Hang (advisor), Behrens, Sven H. (committee member), Breedveld, Victor (committee member), Goldman, Daniel (committee member), Taite, Lakeshia J. (committee member).

Subjects/Keywords: Microfluidics; C. elgans; Dynamic studies; Developmental observation; Continuous live imaging; Lifespan; Caenorhabditis elegans; Molecular biology Automation; Computer vision; High resolution imaging; Microorganisms Imaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krajniak, J. (2013). Microfluidic toolkit for scalable live imaging, developmental and lifespan dynamic studies of C. elegans with single animal resolution. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/49115

Chicago Manual of Style (16^th Edition):

Krajniak, Jan. “Microfluidic toolkit for scalable live imaging, developmental and lifespan dynamic studies of C. elegans with single animal resolution.” 2013. Doctoral Dissertation, Georgia Tech. Accessed April 17, 2021. http://hdl.handle.net/1853/49115.

MLA Handbook (7^th Edition):

Krajniak, Jan. “Microfluidic toolkit for scalable live imaging, developmental and lifespan dynamic studies of C. elegans with single animal resolution.” 2013. Web. 17 Apr 2021.

Vancouver:

Krajniak J. Microfluidic toolkit for scalable live imaging, developmental and lifespan dynamic studies of C. elegans with single animal resolution. [Internet] [Doctoral dissertation]. Georgia Tech; 2013. [cited 2021 Apr 17]. Available from: http://hdl.handle.net/1853/49115.

Council of Science Editors:

Krajniak J. Microfluidic toolkit for scalable live imaging, developmental and lifespan dynamic studies of C. elegans with single animal resolution. [Doctoral Dissertation]. Georgia Tech; 2013. Available from: http://hdl.handle.net/1853/49115

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