+publisher:"Georgia Tech" +contributor:("Sheldon W. May")

Georgia Tech

1. Oldham, Charlie Daniel. Mechanistic studies on protocatechuate 3, 4-dioxygenase.

Degree: PhD, Chemistry, 1983, Georgia Tech

URL: http://hdl.handle.net/1853/29989

Subjects/Keywords: Oxygenases

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APA (6^th Edition):

Oldham, C. D. (1983). Mechanistic studies on protocatechuate 3, 4-dioxygenase. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/29989

Chicago Manual of Style (16^th Edition):

Oldham, Charlie Daniel. “Mechanistic studies on protocatechuate 3, 4-dioxygenase.” 1983. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/29989.

MLA Handbook (7^th Edition):

Oldham, Charlie Daniel. “Mechanistic studies on protocatechuate 3, 4-dioxygenase.” 1983. Web. 05 Mar 2021.

Vancouver:

Oldham CD. Mechanistic studies on protocatechuate 3, 4-dioxygenase. [Internet] [Doctoral dissertation]. Georgia Tech; 1983. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/29989.

Council of Science Editors:

Oldham CD. Mechanistic studies on protocatechuate 3, 4-dioxygenase. [Doctoral Dissertation]. Georgia Tech; 1983. Available from: http://hdl.handle.net/1853/29989

Georgia Tech

2. Ping, Dongsheng. Peptidylamidoglycolate lyase : discovery and characterization.

Degree: PhD, Chemistry and biochemistry, 1994, Georgia Tech

URL: http://hdl.handle.net/1853/30012

Subjects/Keywords: Peptides

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APA (6^th Edition):

Ping, D. (1994). Peptidylamidoglycolate lyase : discovery and characterization. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30012

Chicago Manual of Style (16^th Edition):

Ping, Dongsheng. “Peptidylamidoglycolate lyase : discovery and characterization.” 1994. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/30012.

MLA Handbook (7^th Edition):

Ping, Dongsheng. “Peptidylamidoglycolate lyase : discovery and characterization.” 1994. Web. 05 Mar 2021.

Vancouver:

Ping D. Peptidylamidoglycolate lyase : discovery and characterization. [Internet] [Doctoral dissertation]. Georgia Tech; 1994. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/30012.

Council of Science Editors:

Ping D. Peptidylamidoglycolate lyase : discovery and characterization. [Doctoral Dissertation]. Georgia Tech; 1994. Available from: http://hdl.handle.net/1853/30012

Georgia Tech

3. Edwards, Alise Sarah. An investigation of the in vivo effects of DBM effectors on endogenous cathecholamine metabolism.

Degree: MS, Chemistry, 1987, Georgia Tech

URL: http://hdl.handle.net/1853/30043

Subjects/Keywords: Sympathomimetic agents

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APA (6^th Edition):

Edwards, A. S. (1987). An investigation of the in vivo effects of DBM effectors on endogenous cathecholamine metabolism. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30043

Chicago Manual of Style (16^th Edition):

Edwards, Alise Sarah. “An investigation of the in vivo effects of DBM effectors on endogenous cathecholamine metabolism.” 1987. Masters Thesis, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/30043.

MLA Handbook (7^th Edition):

Edwards, Alise Sarah. “An investigation of the in vivo effects of DBM effectors on endogenous cathecholamine metabolism.” 1987. Web. 05 Mar 2021.

Vancouver:

Edwards AS. An investigation of the in vivo effects of DBM effectors on endogenous cathecholamine metabolism. [Internet] [Masters thesis]. Georgia Tech; 1987. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/30043.

Council of Science Editors:

Edwards AS. An investigation of the in vivo effects of DBM effectors on endogenous cathecholamine metabolism. [Masters Thesis]. Georgia Tech; 1987. Available from: http://hdl.handle.net/1853/30043

Georgia Tech

4. Feng, Jun. A kinetic investigation of recombinant xenopus laevis amidating enzymes.

Degree: PhD, Chemistry and biochemistry, 1999, Georgia Tech

URL: http://hdl.handle.net/1853/30084

Subjects/Keywords: Neuropeptides Synthesis; Peptide hormones; Enzyme inhibitors; Amides

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APA (6^th Edition):

Feng, J. (1999). A kinetic investigation of recombinant xenopus laevis amidating enzymes. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30084

Chicago Manual of Style (16^th Edition):

Feng, Jun. “A kinetic investigation of recombinant xenopus laevis amidating enzymes.” 1999. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/30084.

MLA Handbook (7^th Edition):

Feng, Jun. “A kinetic investigation of recombinant xenopus laevis amidating enzymes.” 1999. Web. 05 Mar 2021.

Vancouver:

Feng J. A kinetic investigation of recombinant xenopus laevis amidating enzymes. [Internet] [Doctoral dissertation]. Georgia Tech; 1999. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/30084.

Council of Science Editors:

Feng J. A kinetic investigation of recombinant xenopus laevis amidating enzymes. [Doctoral Dissertation]. Georgia Tech; 1999. Available from: http://hdl.handle.net/1853/30084

Georgia Tech

5. Moore, Allison Leigh Burrows. An investigation of xenopus laevis skin amidating enzymes expressed in the baculovirus/insect cell system.

Degree: PhD, Chemistry and biochemistry, 1997, Georgia Tech

URL: http://hdl.handle.net/1853/30727

Subjects/Keywords: Xenopus laevis; Baculoviruses

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APA (6^th Edition):

Moore, A. L. B. (1997). An investigation of xenopus laevis skin amidating enzymes expressed in the baculovirus/insect cell system. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30727

Chicago Manual of Style (16^th Edition):

Moore, Allison Leigh Burrows. “An investigation of xenopus laevis skin amidating enzymes expressed in the baculovirus/insect cell system.” 1997. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/30727.

MLA Handbook (7^th Edition):

Moore, Allison Leigh Burrows. “An investigation of xenopus laevis skin amidating enzymes expressed in the baculovirus/insect cell system.” 1997. Web. 05 Mar 2021.

Vancouver:

Moore ALB. An investigation of xenopus laevis skin amidating enzymes expressed in the baculovirus/insect cell system. [Internet] [Doctoral dissertation]. Georgia Tech; 1997. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/30727.

Council of Science Editors:

Moore ALB. An investigation of xenopus laevis skin amidating enzymes expressed in the baculovirus/insect cell system. [Doctoral Dissertation]. Georgia Tech; 1997. Available from: http://hdl.handle.net/1853/30727

Georgia Tech

6. Sirimanne, Sarath Ranjith. Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase.

Degree: PhD, Chemistry and biochemistry, 1988, Georgia Tech

URL: http://hdl.handle.net/1853/30937

Subjects/Keywords: Dopamine; Phenylalanine; Organic compounds Synthesis

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APA (6^th Edition):

Sirimanne, S. R. (1988). Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/30937

Chicago Manual of Style (16^th Edition):

Sirimanne, Sarath Ranjith. “Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase.” 1988. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/30937.

MLA Handbook (7^th Edition):

Sirimanne, Sarath Ranjith. “Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase.” 1988. Web. 05 Mar 2021.

Vancouver:

Sirimanne SR. Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase. [Internet] [Doctoral dissertation]. Georgia Tech; 1988. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/30937.

Council of Science Editors:

Sirimanne SR. Syntheses and enzymatic studies of novel substrate analogs of phenylalanine hydroxylase and dopamine beta monooxygenase. [Doctoral Dissertation]. Georgia Tech; 1988. Available from: http://hdl.handle.net/1853/30937

Georgia Tech

7. Tulsyan, Anurag S. Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory system.

Degree: MS, Chemistry and biochemistry, 2001, Georgia Tech

URL: http://hdl.handle.net/1853/31014

Subjects/Keywords: Developmental genetics; Molecular cloning; Loblolly pine

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APA (6^th Edition):

Tulsyan, A. S. (2001). Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory system. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/31014

Chicago Manual of Style (16^th Edition):

Tulsyan, Anurag S. “Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory system.” 2001. Masters Thesis, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/31014.

MLA Handbook (7^th Edition):

Tulsyan, Anurag S. “Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory system.” 2001. Web. 05 Mar 2021.

Vancouver:

Tulsyan AS. Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory system. [Internet] [Masters thesis]. Georgia Tech; 2001. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/31014.

Council of Science Editors:

Tulsyan AS. Molecular cloning and characterization of a novel developmental gene from loblolly pine - a new plant regulatory system. [Masters Thesis]. Georgia Tech; 2001. Available from: http://hdl.handle.net/1853/31014

8. Kosa, Matyas. Direct and multistep conversion of lignin to biofuels.

Degree: PhD, Chemistry and Biochemistry, 2012, Georgia Tech

URL: http://hdl.handle.net/1853/45836

► Lignin is the second most abundant biopolymer on Earth, right after cellulose, with a highly complex chemical structure that hinders its possible utilizations. Applications that… (more)

Subjects/Keywords: Lignin; Lipid; Oleaginous bateria; Rhodococcus; Beta-ketoadipate; Pyrolysis; Biomass conversion; Biomass energy; Energy crops

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APA (6^th Edition):

Kosa, M. (2012). Direct and multistep conversion of lignin to biofuels. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/45836

Chicago Manual of Style (16^th Edition):

Kosa, Matyas. “Direct and multistep conversion of lignin to biofuels.” 2012. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/45836.

MLA Handbook (7^th Edition):

Kosa, Matyas. “Direct and multistep conversion of lignin to biofuels.” 2012. Web. 05 Mar 2021.

Vancouver:

Kosa M. Direct and multistep conversion of lignin to biofuels. [Internet] [Doctoral dissertation]. Georgia Tech; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/45836.

Council of Science Editors:

Kosa M. Direct and multistep conversion of lignin to biofuels. [Doctoral Dissertation]. Georgia Tech; 2012. Available from: http://hdl.handle.net/1853/45836

9. Ovat, Asli. Design, synthesis and evaluation of cysteine protease inhibitors.

Degree: PhD, Chemistry and Biochemistry, 2009, Georgia Tech

URL: http://hdl.handle.net/1853/33822

► Cysteine proteases are important drug targets due to their involvement in many biological processes such as protein turnover, digestion, blood coagulation, apoptosis, cell differentiation, cell… (more)

▼ Cysteine proteases are important drug targets due to their involvement in many biological processes such as protein turnover, digestion, blood coagulation, apoptosis, cell differentiation, cell signaling, and the immune response. In this thesis, we have reported the design, synthesis and evaluation of clan CA and clan CD cysteine protease inhibitors. Aza-peptidyl Michael acceptor and epoxide inhibitors for asparaginyl endopeptidases (legumains) from the bloodfluke, Schistosoma mansoni (SmAE) and the hard tick, Ixodes ricinus (IrAE) were designed and synthesized. SARs were similar, but with some notable exceptions. Both enzymes prefer disubstituted amides to monosubstituted amides in the P1' position and potency increased as we increased the hydrophobicity of the inhibitor in this position. Extending the inhibitor to P5 resulted in increased inhibitory potency, especially against IrAE, and both enzymes prefer small over large hydrophobic residues in the P2 position. Aza-peptide Michael acceptor inhibitors are more potent than aza-peptide epoxide inhibitors and, for some of these compounds, second order inhibition rate constants are the fastest yet discovered. We have also synthesized aza-peptidyl Michael acceptor and epoxide inhibitors for the parasitic cysteine proteases; cruzain, rhodesain. We have found that monosubstituted amides were favored over disubstituted amides indicating the involvement of the amide hydrogen in a H-bond network. We have shown that aza-peptide epoxides were as potent as Michael acceptors and we have obtained compounds with IC50 values as low as 20 nM. We have worked on the synthesis of heterocyclic peptidyl α-ketoamides, peptidyl ketones and aza-peptidyl ketones as calpain inhibitors. We have synthesized peptidyl α-ketoamides with nucleotide bases in the primed region to create compounds that can cross the blood-brain barrier. We have improved the potency by introducing a hydrophobic group on the adenine ring. We have obtained compounds with Ki values in the nanomolar range. We have designed peptidyl aminoketones as a new class of inhibitors for calpain. Peptidyl aminoketones were less potent than peptidyl α-ketoamides but still reasonable inhibitors of calpain that have the potential to cross the BBB. Advisors/Committee Members: Powers, James C. (Committee Chair), Christoph J. Fahrni (Committee Member), Jonathan D. Glass (Committee Member), Nicholas V. Hud (Committee Member), Sheldon W. May (Committee Member).

Subjects/Keywords: Legumain; Calpain; Protease; Cysteine; Cysteine proteinases; Cysteine proteinases Inhibitors; Protease inhibitors; Biosynthesis; Epoxy compounds

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APA (6^th Edition):

Ovat, A. (2009). Design, synthesis and evaluation of cysteine protease inhibitors. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/33822

Chicago Manual of Style (16^th Edition):

Ovat, Asli. “Design, synthesis and evaluation of cysteine protease inhibitors.” 2009. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/33822.

MLA Handbook (7^th Edition):

Ovat, Asli. “Design, synthesis and evaluation of cysteine protease inhibitors.” 2009. Web. 05 Mar 2021.

Vancouver:

Ovat A. Design, synthesis and evaluation of cysteine protease inhibitors. [Internet] [Doctoral dissertation]. Georgia Tech; 2009. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/33822.

Council of Science Editors:

Ovat A. Design, synthesis and evaluation of cysteine protease inhibitors. [Doctoral Dissertation]. Georgia Tech; 2009. Available from: http://hdl.handle.net/1853/33822

Georgia Tech

10. Burns, Kristi Lee. An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses.

Degree: PhD, Chemistry and Biochemistry, 2006, Georgia Tech

URL: http://hdl.handle.net/1853/29593

► We now report an in depth analysis of the successful in vitro enzymatic synthesis of PHB utilizing the three-enzyme system from the bacteria Cupriavidus necator.… (more)

▼ We now report an in depth analysis of the successful in vitro enzymatic synthesis of PHB utilizing the three-enzyme system from the bacteria Cupriavidus necator. Using HPLC methodology developed in this laboratory, and by adding each enzyme in a step-wise manner, we follow each individual stage in the three-enzyme route for PHB synthesis and delineate all stoichiometric relationships. We report the construction of the first metabolic model developed specifically for analyzing in vitro enzymatic PHB synthesis. We developed a hands-on student laboratory for culturing, producing, isolating, and purifying the bacterial biopolyesters PHB. We now report the first structural characterizations of iso-CoA, acetyl-iso-CoA, acetoacetyl-iso-CoA, and beta-hydroxybutyryl-iso-CoA using MS, MS/MS, and homo- and hetero-nuclear NMR analyses.We describe HPLC methodology to separate the isomers of several iso-CoA-containing compounds and report the first examples of iso-CoA-containing compounds acting as substrates in enzymatic acyl-transfer reactions. We describe a simple regioselective synthesis of iso-CoA from CoA. We also demonstrate a plausible mechanism, which accounts for the existence of iso-CoA isomers in commercial preparations of CoA-containing compounds. Herein we report that phenylaminoethyl selenide compounds protect DNA from peroxynitrite-mediated single-strand breaks. The mechanism of protection against peroxynitrite mediated DNA damage was investigated by HPLC. The chemistry of the reaction between peroxynitrite and HOMePAES was investigated using HPLC and HPLC/MS. The unique chemistry of the reaction between peroxynitrite and HOMePAES was investigated using HPLC and HPLC/MS. We report the development of novel CDB derivatives, which are selective COX-II inhibitors. A series of compounds were assayed with an in vitro colorimetric inhibitor screening and with a whole blood ELISA screening and the results indicate that MST is a selective inhibitor of COX-II. Advisors/Committee Members: Sheldon W. May (Committee Chair), Donald F. Doyle, Leslie T. Gelbaum, Stanley H. Pollock, and James Powers (Committee Members).

Subjects/Keywords: Iso-coa; Iso-coenzyme A; Poly-(beta)-hydroxybutyric acid; PHB; Phenylaminoethyl selenide compounds; Biopolyesters; Acetyl-iso-coa; Acetoacetyl-iso-coa; Regioselective synthesis; Homepaes; DNA; DNA damage; Single-strand breaks; Acyl-transfer reactions; Culturing; Homo-nuclear NMR; Purifying; Producing; Isolating; Isomers; Selective COX-II inhibitors; Whole blood; Structural characterizations; ELISA; In vitro; Enzymatic synthesis; Beta-hydroxybutyryl-iso-coa; MS/MS; MS; HPLC; Metabolic model; Hetero-nuclear NMR; Peroxynitrite; HPLC/MS; CDB derivatives; Poly-beta-hydroxybutyrate; Biosynthesis

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APA (6^th Edition):

Burns, K. L. (2006). An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/29593

Chicago Manual of Style (16^th Edition):

Burns, Kristi Lee. “An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses.” 2006. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/29593.

MLA Handbook (7^th Edition):

Burns, Kristi Lee. “An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses.” 2006. Web. 05 Mar 2021.

Vancouver:

Burns KL. An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses. [Internet] [Doctoral dissertation]. Georgia Tech; 2006. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/29593.

Council of Science Editors:

Burns KL. An exploration of biochemistry including biotechnology, structural characterization, drug design, and chromatographic analyses. [Doctoral Dissertation]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/29593

Georgia Tech

11. Chen, Yanfeng. Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry.

Degree: PhD, Chemistry and Biochemistry, 2006, Georgia Tech

URL: http://hdl.handle.net/1853/14620

► Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) is a novel technique for direct analysis of organic and biological molecules. Amino acids, dipeptides, and organoselenium compounds… (more)

▼ Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) is a novel technique for direct analysis of organic and biological molecules. Amino acids, dipeptides, and organoselenium compounds were successfully detected by SALDI on carbon and silicon surfaces. Surface effects, solvent effects, temperature effects and pH effects were studied. A possible mechanism of SALDI is proposed based on observed results. In general, stimulated desorption results in neutral yields that are much larger than ion yields. Thus, we have exploited and further developed laser desorption single photon ionization mass spectrometry (LD/SPI MS) as a means of examining biomolecules. The experimental results clearly demonstrate that LD/SPI MS is a very useful and fast analysis method with uniform selectivity and high sensitivity. Selenium (Se) is an essential ultra-trace element in the human body. In efforts to obtain more useful information of selenium metabolites in human urine, mass determination of unknown organoselenium compounds in biological matrices using SALDI MS was investigated. In another approach, several selenium metabolites in human urine were successfully detected by LD/SPI MS. A HPLC-MS/MS method was also developed for a quantitative case study of selenium metabolites in human urine after ingestion of selenomethionine. Low-energy electrons (LEE, 3-20 eV) have been shown to induce single and double strand breaks (SSB and DSB) in plasmid DNA. To understand the genotoxic effects due to secondary species of high-energy radiation, we investigate the role of transient negative ions and the specificity in LEE-DNA damage by examining the neutral product yields using low electron stimulated dissociation SPI MS. The neutral yields as a function of incident electron energy are also correlated with the SSBs and DSBs measured using post-irradiation gel electrophoresis. The results provide further insight concerning the mechanisms of LEE-induced damage to DNA. Overall, this research provided an in-depth understanding of non-thermal surface processes and the development of new mass spectrometric techniques for the analysis of biomolecules. Advisors/Committee Members: Thomas M. Orlando (Committee Chair), Alfred Merrill, Jr. (Committee Member), Facundo M. Fernandez (Committee Member), Mostafa A. El-Sayed (Committee Member), Sheldon W. May (Committee Member).

Subjects/Keywords: Single photon ionization; Selenium metabolites; Electron stimulated desorption; DNA damage; Surface-assisted laser desorption/ionization; Mass spectrometry

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APA (6^th Edition):

Chen, Y. (2006). Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/14620

Chicago Manual of Style (16^th Edition):

Chen, Yanfeng. “Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry.” 2006. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/14620.

MLA Handbook (7^th Edition):

Chen, Yanfeng. “Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry.” 2006. Web. 05 Mar 2021.

Vancouver:

Chen Y. Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry. [Internet] [Doctoral dissertation]. Georgia Tech; 2006. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/14620.

Council of Science Editors:

Chen Y. Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry. [Doctoral Dissertation]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/14620

Georgia Tech

12. Azizi, Bahareh. Chemical Complementation: A Genetic Selection System in Yeast for Drug Discovery, Protein Engineering, and for Deciphering and Assembling Biosynthetic Pathways.

Degree: PhD, Chemistry and Biochemistry, 2005, Georgia Tech

URL: http://hdl.handle.net/1853/11637

► Chemical complementation is a general system for detecting protein-small molecule interactions, and linking that interaction to genetic selection. In this chemical complementation system, the interaction… (more)

▼ Chemical complementation is a general system for detecting protein-small molecule interactions, and linking that interaction to genetic selection. In this chemical complementation system, the interaction of a nuclear receptor and a ligand is essential for yeast survival. In first generation chemical complementation, a two-component assay was developed where the Gal4 DNA-binding domain is fused to the ligand binding domains of nuclear receptors, and expressed in the strain S.cerevisiae PJ69-4A. The Gal4 DNA binding domain binds to a Gal4 response element controlling transcription of a selective marker, and the nuclear receptor ligand-binding domain binds its ligand. This system was developed using the retinoid X receptor, the pregnane X receptor, and the liver X receptor and their ligands 9-cis retinoic acid, paclitaxel, and oxysterols, respectively. Yeast survive on selective plates only in the presence of both components: a nuclear receptor and the corresponding ligand. Growth was observed at the highest concentration of ligand (10-5 M) and, compared to Gal4-activated growth, the growth density was less and growth time was more. The second generation chemical complementation system is a three-component system comprising a nuclear receptor ligand-binding domain fused to the Gal4 DNA binding domain, the ligand, and a nuclear receptor coactivator fused to the yeast Gal4 activation domain. This system was developed using the retinoid X receptor and has been extended to several other nuclear receptors. The sensitivity of chemical complementation is increased 1000-fold, and growth time and density are equivalent to Gal4-activated growth. An assay was developed to provide a quantitative high-throughput assay for evaluating nuclear receptor- ligand interactions, and measuring EC50 values for the ligand-receptor pairs. Chemical complementation can be used in a variety of applications, such as drug discovery for nuclear receptor-based disease, providing a high-throughput assay for the discovery of potential nuclear receptor agonists, and with the use of the negative chemical complementation system, the discovery of nuclear receptor antagonists. Chemical complementation is used for protein engineering, specifically engineering receptors to bind and activate in response to other ligands. Chemical complementation is also used for deciphering and assembling biosynthetic pathways. Advisors/Committee Members: Donald F. Doyle (Committee Chair), Allen M. Orville (Committee Member), Jung H. Choi (Committee Member), Mostafa A. El-Sayed (Committee Member), Sheldon W. May (Committee Member).

Subjects/Keywords: Enzyme engineering; Chemical genetics

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APA (6^th Edition):

Azizi, B. (2005). Chemical Complementation: A Genetic Selection System in Yeast for Drug Discovery, Protein Engineering, and for Deciphering and Assembling Biosynthetic Pathways. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/11637

Chicago Manual of Style (16^th Edition):

Azizi, Bahareh. “Chemical Complementation: A Genetic Selection System in Yeast for Drug Discovery, Protein Engineering, and for Deciphering and Assembling Biosynthetic Pathways.” 2005. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/11637.

MLA Handbook (7^th Edition):

Azizi, Bahareh. “Chemical Complementation: A Genetic Selection System in Yeast for Drug Discovery, Protein Engineering, and for Deciphering and Assembling Biosynthetic Pathways.” 2005. Web. 05 Mar 2021.

Vancouver:

Azizi B. Chemical Complementation: A Genetic Selection System in Yeast for Drug Discovery, Protein Engineering, and for Deciphering and Assembling Biosynthetic Pathways. [Internet] [Doctoral dissertation]. Georgia Tech; 2005. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/11637.

Council of Science Editors:

Azizi B. Chemical Complementation: A Genetic Selection System in Yeast for Drug Discovery, Protein Engineering, and for Deciphering and Assembling Biosynthetic Pathways. [Doctoral Dissertation]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/11637

Georgia Tech

13. Foster, Michael Scott. Design, synthesis, kinetic analysis, molecular modeling, and pharmacological evaluation of novel inhibitors of peptide amidation.

Degree: PhD, Chemistry and Biochemistry, 2008, Georgia Tech

URL: http://hdl.handle.net/1853/31816

► Novel, rationally-designed acrylate analogs of various known dipeptide substrates were found to be mechanism-based inactivators of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM, EC 1.14.17.3). This… (more)

Subjects/Keywords: Molecular modeling; Stereochemistry; Molecules Models; Peptides; Chemical structure; Peptide hormones; Neuropeptides

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APA (6^th Edition):

Foster, M. S. (2008). Design, synthesis, kinetic analysis, molecular modeling, and pharmacological evaluation of novel inhibitors of peptide amidation. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/31816

Chicago Manual of Style (16^th Edition):

Foster, Michael Scott. “Design, synthesis, kinetic analysis, molecular modeling, and pharmacological evaluation of novel inhibitors of peptide amidation.” 2008. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/31816.

MLA Handbook (7^th Edition):

Foster, Michael Scott. “Design, synthesis, kinetic analysis, molecular modeling, and pharmacological evaluation of novel inhibitors of peptide amidation.” 2008. Web. 05 Mar 2021.

Vancouver:

Foster MS. Design, synthesis, kinetic analysis, molecular modeling, and pharmacological evaluation of novel inhibitors of peptide amidation. [Internet] [Doctoral dissertation]. Georgia Tech; 2008. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/31816.

Council of Science Editors:

Foster MS. Design, synthesis, kinetic analysis, molecular modeling, and pharmacological evaluation of novel inhibitors of peptide amidation. [Doctoral Dissertation]. Georgia Tech; 2008. Available from: http://hdl.handle.net/1853/31816

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Open Access Theses and Dissertations