+publisher:"Georgia Tech" +contributor:("Elliot Chaikof")

1. Martinez, Adam W. Design and development of an elastin mimetic stent with therapeutic delivery potential.

Degree: PhD, Biomedical Engineering, 2011, Georgia Tech

► Stenting remains a common treatment option for atherosclerotic arteries. The main drawback of early stent platforms was restenosis, which has been combated by drug eluting… (more)

Subjects/Keywords: Rat stenting; Elastin miemtic; Laser fabrication; Crosslinking; Drug delivery; Stent; Stents (Surgery); Drug-eluting stents; Drug delivery devices; Implants, Artificial

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martinez, A. W. (2011). Design and development of an elastin mimetic stent with therapeutic delivery potential. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/45926

Chicago Manual of Style (16^th Edition):

Martinez, Adam W. “Design and development of an elastin mimetic stent with therapeutic delivery potential.” 2011. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/45926.

MLA Handbook (7^th Edition):

Martinez, Adam W. “Design and development of an elastin mimetic stent with therapeutic delivery potential.” 2011. Web. 23 Jan 2021.

Vancouver:

Martinez AW. Design and development of an elastin mimetic stent with therapeutic delivery potential. [Internet] [Doctoral dissertation]. Georgia Tech; 2011. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/45926.

Council of Science Editors:

Martinez AW. Design and development of an elastin mimetic stent with therapeutic delivery potential. [Doctoral Dissertation]. Georgia Tech; 2011. Available from: http://hdl.handle.net/1853/45926

2. Coburn, Leslie Ann. Studies of platelet gpib-alpha and von willebrand factor bond formation under flow.

Degree: PhD, Biomedical Engineering, 2010, Georgia Tech

URL: http://hdl.handle.net/1853/39565

► Understanding the differential bonding mechanics underlying bleeding disorders is of crucial importance to human health. In this research insight is provided into how four of… (more)

▼ Understanding the differential bonding mechanics underlying bleeding disorders is of crucial importance to human health. In this research insight is provided into how four of these bleeding disorders (each with somewhat similar clinical characteristics), work at the molecular bond level. The bleeding diseases studied here can result from defects in the platelet glycoprotein (GP) Ibα the von Willebrand factor (vWF) molecule, or the ADAMTS-13 enzyme. Types 2B and 2M von Willebrand Disease (VWD) result in excess bleeding, yet type 2B has increased binding affinity between platelet GPIbα and vWF, while type 2M has decreased binding affinity between these two molecules. Platelet type VWD (pt-VWD) causes mutations in the GPIbα molecule and has similar characteristics to type 2B VWD. Further, in thrombotic thrombocytopenic purpura, bleeding results when there is a lack of active ADAMTS-13 enzyme. Each disease results in patient bleeding, but due to different mechanisms. This dissertation will explore the bonding mechanics between GPIbα and vWF and how they are altered in each disease state. To observe the GPIbα-vWF bonding mechanics, rolling velocities, transient tethering lifetimes, and tether frequency were determined using a parallel plate flow chamber. Data from these experiments suggest that wt-wt interactions are force dependent and have biphasic catch-slip bonding behavior. The data show that the shear stress at which the maximum mean stop time occurs differs between gain-of-function and loss-of-function mutations. Using similar methods, we study the changes resulting from pt-VWD mutations in GPIbα, and find that the catch bond seen for wt-wt interactions is lost for these mutations. Further, the data suggest that interactions with gain-of-function GPIbα mutations may be transport rather than force dependent. Finally, how the GPIbα-vWF tether bond changes for thrombotic thrombocytopenic purpura was also investigated to show that the bond lifetime in the absence of the enzyme is increased presenting a possible rationale for why bleeding occurs in this disease. Overall, the data show how the bonding mechanics of the GPIbα-vWF tether bond differ in four bleeding diseases. Further, these observations offer potential explanations for how these changes in the bonding mechanism may play a role in the observed patient bleeding. Advisors/Committee Members: Larry V. McIntire (Committee Chair), Cheng Zhu (Committee Member), Elliot Chaikof (Committee Member), Miguel Cruz (Committee Member), Suzanne G. Eskin (Committee Member).

Subjects/Keywords: Von Willebrand disease; Von Willebrand factor; GPIba; Platelet adhesion; Catch bonds; Von Willebrand factor; Platelet glycoprotein GPIIb-IIIa complex; Hemorrhagic diseases; Blood platelets Aggregation

8 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Coburn, L. A. (2010). Studies of platelet gpib-alpha and von willebrand factor bond formation under flow. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/39565

Chicago Manual of Style (16^th Edition):

Coburn, Leslie Ann. “Studies of platelet gpib-alpha and von willebrand factor bond formation under flow.” 2010. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/39565.

MLA Handbook (7^th Edition):

Coburn, Leslie Ann. “Studies of platelet gpib-alpha and von willebrand factor bond formation under flow.” 2010. Web. 23 Jan 2021.

Vancouver:

Coburn LA. Studies of platelet gpib-alpha and von willebrand factor bond formation under flow. [Internet] [Doctoral dissertation]. Georgia Tech; 2010. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/39565.

Council of Science Editors:

Coburn LA. Studies of platelet gpib-alpha and von willebrand factor bond formation under flow. [Doctoral Dissertation]. Georgia Tech; 2010. Available from: http://hdl.handle.net/1853/39565

Georgia Tech

3. Sathe, Rahul D. Design and Development of a Novel Implantable Prosthetic Vein Valve.

Degree: MS, Mechanical Engineering, 2006, Georgia Tech

URL: http://hdl.handle.net/1853/14495

► Over seven million Americans suffer from Chronic Venous Insufficiency (CVI), a painful and debilitating disease that affects the superficial and deep veins of the legs.… (more)

▼ Over seven million Americans suffer from Chronic Venous Insufficiency (CVI), a painful and debilitating disease that affects the superficial and deep veins of the legs. Problems associated with CVI include varicose veins, bleeding, ulcerations, severe swelling, deep vein thrombosis, and pulmonary embolism, which may lead to death. The presence of CVI results from damaged (incompetent) one-way vein valves in leg veins. These valves normally allow forward flow of blood to the heart, and prevent blood from pooling at the feet. However, incompetent valves allow reflux of blood, causing clinical problems. There are few effective clinical therapies for treating CVI. Vein valve transplantation is a surgical option for treatment. However, it is often difficult to find suitable donor valves. Very few prosthetic valves developed in the past have demonstrated sufficient clinical or mechanical functionality. Persistent problems include thrombus formation, leaking valves, and valves that do not open at physiologic pressure gradient. The primary objective of this research was to develop a clinically relevant functional prosthetic vein valve. The novel prosthetic valve is flexible, biocompatible, has low thrombogenecity, and is easy to manufacture. It was designed to address well-defined consumer needs and functional design requirements. The valve was required to 1) withstand 300 mmHg of backpressure with leakage less than 1.0 mL/min, 2) open with a pressure gradient less than 5 mmHg, and 3) meet criteria 1 and 2 after 500,000 cycles of operation. The valve met these design requirements in bench testing. The valve can open with a pressure gradient of 2.6 0.7 mmHg, and can withstand 300 mmHg with leakage less than 0.5 mL/min. The valve remained functional after opening and closing over 500,000 times. The valve presented in this research is operationally functional, and is a potential solution for treating venous incompetence in CVI patients. Advisors/Committee Members: Dr. David N. Ku (Committee Chair), Dr. David Rosen (Committee Member), Dr. Elliot Chaikof (Committee Member).

Subjects/Keywords: Vein valve; Deep vein thrombosis; Venous valve; Prosthetic vein valve; Chronic venous insufficiency; Implants, Artificial Design and construction; Venous valves Transplantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sathe, R. D. (2006). Design and Development of a Novel Implantable Prosthetic Vein Valve. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/14495

Chicago Manual of Style (16^th Edition):

Sathe, Rahul D. “Design and Development of a Novel Implantable Prosthetic Vein Valve.” 2006. Masters Thesis, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/14495.

MLA Handbook (7^th Edition):

Sathe, Rahul D. “Design and Development of a Novel Implantable Prosthetic Vein Valve.” 2006. Web. 23 Jan 2021.

Vancouver:

Sathe RD. Design and Development of a Novel Implantable Prosthetic Vein Valve. [Internet] [Masters thesis]. Georgia Tech; 2006. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/14495.

Council of Science Editors:

Sathe RD. Design and Development of a Novel Implantable Prosthetic Vein Valve. [Masters Thesis]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/14495

Georgia Tech

4. Farrell, Laura-Lee Amelia Catherine. Prosthetic Vein Valve: Delivery and In Vitro Evaluation.

Degree: MS, Biomedical Engineering, 2007, Georgia Tech

URL: http://hdl.handle.net/1853/14531

► Venous disease will affect 1-3% of the western world at some point in their lives, yet there are few effective treatments for the venous system… (more)

▼ Venous disease will affect 1-3% of the western world at some point in their lives, yet there are few effective treatments for the venous system [1]. One such disease is chronic venous insufficiency (CVI), a painful and debilitating illness that affects the superficial and deep vein valves of the legs. When the valves become incompetent they allow reflux and subsequent pooling of blood. Current clinical therapies are only moderately; and therefore, the need for a better solution remains. Prosthetic venous valves were constructed from a novel hydrogel biomaterial patented by Georgia Tech. The valves had flexible cusps similar to normal, anatomic venous valves. The purpose of this work was to evaluate the thrombotic potential of the GT venous valve in an in vitro study and to design a percutaneous delivery system. In vitro thrombosis model provides an appropriate intermediate step between valve development and in vivo analysis, which is necessary to determine the biocompatibility of the prosthetic device. The flow system was modified from a one-pass, flow-through thrombosis assay using whole blood [2] to mimic pulsatile physiologic conditions. Cessation of flow indicated thrombotic obstruction. Histological analysis was performed using H and E staining and Carstairs stain (specific for platelets). A group of valves were lined with Dacron to confirm the thrombotic potential of the system. All Dacron valves were occluded by thrombus connecting the polymer fibers with adherent platelets. Whole blood perfused through the GT prosthetic valves exhibited no thrombosis or platelet adherence. All GT valves were patent and competent after blood perfusion. H and E staining revealed no thrombus deposition on the GT vein valves. A percutaneous delivery system was designed after evaluating the GT valves for their compressibility and plastic deformation over time. Appropriate stents, catheters and sheaths were selected. As designed, this system will be utilized in an ovine trial of the valve. Due to the low in vitro thrombotic potential and strong history of PVA as a medical implant material, positive trial results are expected. With successful animal and human trials this valve can provide a potential intervention for the 7 million people suffering from CVI. Advisors/Committee Members: Dr. David Ku (Committee Chair), Dr. Elliot Chaikof (Committee Member), Dr. Ross Milner (Committee Member).

Subjects/Keywords: Flow system; Porcine blood; Vein; Vein valve; Venous; Venous valve; Valves; Thrombus; Thrombotic potential; Platelets; Delivery; Stent; Delivery system; Catheter; Ovine; Sheep; Blood; Deep venous thrombosis; Chronic venous insufficiency

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Farrell, L. A. C. (2007). Prosthetic Vein Valve: Delivery and In Vitro Evaluation. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/14531

Chicago Manual of Style (16^th Edition):

Farrell, Laura-Lee Amelia Catherine. “Prosthetic Vein Valve: Delivery and In Vitro Evaluation.” 2007. Masters Thesis, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/14531.

MLA Handbook (7^th Edition):

Farrell, Laura-Lee Amelia Catherine. “Prosthetic Vein Valve: Delivery and In Vitro Evaluation.” 2007. Web. 23 Jan 2021.

Vancouver:

Farrell LAC. Prosthetic Vein Valve: Delivery and In Vitro Evaluation. [Internet] [Masters thesis]. Georgia Tech; 2007. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/14531.

Council of Science Editors:

Farrell LAC. Prosthetic Vein Valve: Delivery and In Vitro Evaluation. [Masters Thesis]. Georgia Tech; 2007. Available from: http://hdl.handle.net/1853/14531

Georgia Tech

5. Sallach, Rory Elizabeth. Recombinant elastin-mimetic protein polymers as design elements for an arterial substitute.

Degree: PhD, Biomedical Engineering, 2008, Georgia Tech

URL: http://hdl.handle.net/1853/29614

► Recombinant synthesis of elastin-mimetic proteins has been employed for several decades, however, long-term biocompatibility and biostability of such proteins was not fully defined. We present… (more)

▼ Recombinant synthesis of elastin-mimetic proteins has been employed for several decades, however, long-term biocompatibility and biostability of such proteins was not fully defined. We present virtually crosslinked elastin-mimetic proteins which exhibit exceptional biocompatibility and long-term biostability over a period of at least seven months. This report is the first evidence of a non-chemically or ionically crosslinked system that exhibits long-term in vivo stability. Although, physically crosslinked protein-based materials possess a number of advantages over their chemically crosslinked counterparts, physical crosslinks and the related domains so formed may be deformed or damaged at applied stresses lower than those required to disrupt covalent crosslinks. In this regard, we have synthesized a new class of recombinant elastin-mimetic triblock copolymer capable of both physical and chemical crosslinking. We have demonstrated that chemical crosslinking provides an independent mechanism for control of protein mechanical responses. Specifically, elastic modulus was enhanced and creep strain reduced through the addition of chemical crosslinking sites. A number of reports have described the design of synthetic genes, which encode elastin-like proteins for bacterial expression in Escherichia coli. Although advantages with this expression system exist, significant limitations including the lack of eukaryotic post-translational systems, the tendency to sequester mammalian proteins into inclusion bodies, difficult purification protocols, and endotoxin contamination have been noted. We demonstrate the expression of a recombinant elastin-mimetic protein from P. pastoris. A novel synthetic strategy, monomer library concatamerization, was utilized in designing non-repetitive elastin genes for highly repetitive protein sequences. It is likely that this strategy will be useful for creating large, repetitive genes for a variety of expression systems in order to more closely approach the genetic diversity inherent to native DNA sequences. All told, elastin-based protein polymers are a promising class of material characterized by high degree of biocompatibility, excellent biostability, and a tunable range of mechanical properties from plastic to elastic. A variety of options facilitate the processing of these biopolymers into chemically crosslinked or non-crosslinked gels, films, or nanofibers for any of a number of implant applications including structural components of artificial organs and engineered living tissues, carriers for controlled drug release, or biocompatible surface coatings. Advisors/Committee Members: Elliot Chaikof (Committee Chair), Marc Levenston (Committee Member), Robert Nerem (Committee Member), Vincent Conticello (Committee Member), Yadong Wang (Committee Member).

Subjects/Keywords: Mechanical testing; Protein experession; Biostability; Biocompatibility; Genetic engineering; Recombinant elastin; Crosslinking (Polymerization); Recombinant proteins; Polytef; Vascular grafts; Blood vessel prosthesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sallach, R. E. (2008). Recombinant elastin-mimetic protein polymers as design elements for an arterial substitute. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/29614

Chicago Manual of Style (16^th Edition):

Sallach, Rory Elizabeth. “Recombinant elastin-mimetic protein polymers as design elements for an arterial substitute.” 2008. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/29614.

MLA Handbook (7^th Edition):

Sallach, Rory Elizabeth. “Recombinant elastin-mimetic protein polymers as design elements for an arterial substitute.” 2008. Web. 23 Jan 2021.

Vancouver:

Sallach RE. Recombinant elastin-mimetic protein polymers as design elements for an arterial substitute. [Internet] [Doctoral dissertation]. Georgia Tech; 2008. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/29614.

Council of Science Editors:

Sallach RE. Recombinant elastin-mimetic protein polymers as design elements for an arterial substitute. [Doctoral Dissertation]. Georgia Tech; 2008. Available from: http://hdl.handle.net/1853/29614

Georgia Tech

6. Stabler, Cheryl Lynn. Development of Noninvasive Methods for Monitoring Tissue Engineered Constructs using Nuclear Magnetic Resonance.

Degree: PhD, Biomedical Engineering, 2004, Georgia Tech

URL: http://hdl.handle.net/1853/5239

► Implanted tissue engineered substitutes constitute dynamic systems, with remodeling mediated by both the implanted cells and the host. Thus, there exists a significant need for… (more)

▼ Implanted tissue engineered substitutes constitute dynamic systems, with remodeling mediated by both the implanted cells and the host. Thus, there exists a significant need for methods to monitor the function and morphology of tissue engineered constructs. Noninvasive monitoring using 1H Nuclear Magnetic Resonance (NMR) spectroscopy and imaging can prove to be the solution to this problem. Spectroscopy allows for assessment of cellular function through the monitoring of inherent metabolic markers, such as total-choline, while high resolution imaging enables the evaluation of construct morphology and interfacial remodeling. We applied these 1H NMR methods to monitor betaTC3 mouse insulinoma cells within hydrogel-based materials as a model pancreatic tissue substitute. In vitro research established a strong correlation between total-choline, measured by 1H NMR spectroscopy, and viable betaTC3 cell number, measured by MTT. Extending these methods to in vivo monitoring, however, was met with additional challenges. First, the implanted cells needed to be contained within a planar construct above a threshold density to allow for adequate quantification of the total-choline peak. Secondly, cell-free buffer zones between the implanted cells and the host tissue needed to be incorporated to prevent host tissue signal contamination. Finally, quantitative techniques needed to be developed to accurately account for contaminating signal from diffusing molecules. To overcome these challenges, a disk-shaped agarose construct, initially containing a minimum of 4 million betaTC3 cells and coated with an outer layer of pure agarose, was fabricated. Mathematical simulations aided the implant design by characterizing diffusive transport of nutrients and metabolites into and out of the construct. In vivo 1H NMR studies of these constructs implanted in mice established a strong correlation between total-choline, measured noninvasively using 1H NMR spectroscopy, and viable cell number, measured invasively using MTT. This study establishes total-choline as a reliable marker for noninvasively quantifying dynamic changes in viable betaTC3 cell number in vivo. 1H NMR imaging was used to monitor the implants structural integrity over time, while also assessing the hosts fibrotic response. We expect these studies to establish quantitative criteria for the capabilities and limitations of NMR methodologies for monitoring encapsulated insulinomas, as well as other tissue implants. Advisors/Committee Members: Athanassios Sambanis (Committee Chair), Elliot Chaikof (Committee Member), Ioannis Constantinidis (Committee Member), Robert C Long, Jr (Committee Member), Stephen Hanson (Committee Member).

Subjects/Keywords: Agarose; NMR; Bioartificial pancreas; Alginate; Spectrum analysis; Pancreas Imaging; Nuclear magnetic resonance; Magnetic resonance imaging; Diagnostic imaging; Alginates

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stabler, C. L. (2004). Development of Noninvasive Methods for Monitoring Tissue Engineered Constructs using Nuclear Magnetic Resonance. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/5239

Chicago Manual of Style (16^th Edition):

Stabler, Cheryl Lynn. “Development of Noninvasive Methods for Monitoring Tissue Engineered Constructs using Nuclear Magnetic Resonance.” 2004. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/5239.

MLA Handbook (7^th Edition):

Stabler, Cheryl Lynn. “Development of Noninvasive Methods for Monitoring Tissue Engineered Constructs using Nuclear Magnetic Resonance.” 2004. Web. 23 Jan 2021.

Vancouver:

Stabler CL. Development of Noninvasive Methods for Monitoring Tissue Engineered Constructs using Nuclear Magnetic Resonance. [Internet] [Doctoral dissertation]. Georgia Tech; 2004. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/5239.

Council of Science Editors:

Stabler CL. Development of Noninvasive Methods for Monitoring Tissue Engineered Constructs using Nuclear Magnetic Resonance. [Doctoral Dissertation]. Georgia Tech; 2004. Available from: http://hdl.handle.net/1853/5239

Georgia Tech

7. Capadona, Jeffrey R. Surface-directed assembly of fibrillar extracellular matrices.

Degree: PhD, Chemistry and Biochemistry, 2005, Georgia Tech

URL: http://hdl.handle.net/1853/10575

► Biologically-inspired materials have emerged as promising substrates for enhanced repair in various therapeutic and regenerative medicine applications, including nervous and vascular tissues, bone, and cartilage.… (more)

Subjects/Keywords: Fibronectin; Biomimetics; Biomaterials; Extracellular matrices; Self-assembled monolayers

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Capadona, J. R. (2005). Surface-directed assembly of fibrillar extracellular matrices. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/10575

Chicago Manual of Style (16^th Edition):

Capadona, Jeffrey R. “Surface-directed assembly of fibrillar extracellular matrices.” 2005. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/10575.

MLA Handbook (7^th Edition):

Capadona, Jeffrey R. “Surface-directed assembly of fibrillar extracellular matrices.” 2005. Web. 23 Jan 2021.

Vancouver:

Capadona JR. Surface-directed assembly of fibrillar extracellular matrices. [Internet] [Doctoral dissertation]. Georgia Tech; 2005. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/10575.

Council of Science Editors:

Capadona JR. Surface-directed assembly of fibrillar extracellular matrices. [Doctoral Dissertation]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/10575

Georgia Tech

8. Yoshida, Mutsumi. Mechanism of biomaterial adjuvant effect: Phenotype of dendritic cells upon biomaterial contact.

Degree: PhD, Biomedical Engineering, 2005, Georgia Tech

URL: http://hdl.handle.net/1853/7244

► Development of combination products such as tissue engineered constructs which combine biomaterials with biologics has prompted the need to clarify the role of biomaterial in… (more)

▼ Development of combination products such as tissue engineered constructs which combine biomaterials with biologics has prompted the need to clarify the role of biomaterial in potentiating the immune response towards the biological component due to adjuvant effect. In tissue engineering applications, immune responses are to be minimized while vaccine strategies seek to enhance the protective immune response. Thesis project presented herein showed that adjuvant effect of poly(lactic-co-glycolic acid) (PLGA) is mediated in part by maturation of dendritic cells (DCs), immune cells that orchestrate adaptive immune response. Maturation of human peripheral blood monocyte-derived DCs in response to PLGA contact was demonstrated in vitro and in vivo by increased co-stimulatory and MHC molecule expression, mixed lymphocyte reaction, cytokine release, and delayed type hypersensitivity reaction. In contrast to PLGA, agarose did not induce DC maturation, in accordance with its low inflammatory effect. Roles of various receptors involved in DC maturation and recognition of biomaterials were assessed by in vitro receptor blocking studies. In particular, role of Toll-like receptors were further investigated using DCs derived from bone marrows of murine model of Toll-like receptor 4 deficiency (C3H/HeJ). While PLGA induced maturation of DCs from C57BL6 mice, maturation was not observed in DCs from C3H/HeJ strain or control strain, C3H/HeOuJ, perhaps due to particular haplotypes of these animals. Collectively, these results establish the differential adjuvant effects of agarose and PLGA on the level of DC maturation, and begin to elucidate the mechanisms of biomaterial adjuvant effect. In addition, assays developed herein provide methods to screen for biomaterials to be used in combination products, such that biomaterials with desired levels of adjuvanticity as measured by DC maturation effects may be selected for given application. Advisors/Committee Members: Babensee, Julia (Committee Chair), Andres Garcia (Committee Member), Barbara Boyan (Committee Member), Cheng Zhu (Committee Member), Elliot Chaikof (Committee Member), Mary Marovich (Committee Member).

Subjects/Keywords: Biomaterials; Adjuvant; Dendritic cell; Immune response; Combination product; Tissue engineering; Immunological adjuvants; Tissue engineering; Biologicals; Biomedical materials; Dendritic cells; Immune response

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yoshida, M. (2005). Mechanism of biomaterial adjuvant effect: Phenotype of dendritic cells upon biomaterial contact. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/7244

Chicago Manual of Style (16^th Edition):

Yoshida, Mutsumi. “Mechanism of biomaterial adjuvant effect: Phenotype of dendritic cells upon biomaterial contact.” 2005. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/7244.

MLA Handbook (7^th Edition):

Yoshida, Mutsumi. “Mechanism of biomaterial adjuvant effect: Phenotype of dendritic cells upon biomaterial contact.” 2005. Web. 23 Jan 2021.

Vancouver:

Yoshida M. Mechanism of biomaterial adjuvant effect: Phenotype of dendritic cells upon biomaterial contact. [Internet] [Doctoral dissertation]. Georgia Tech; 2005. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/7244.

Council of Science Editors:

Yoshida M. Mechanism of biomaterial adjuvant effect: Phenotype of dendritic cells upon biomaterial contact. [Doctoral Dissertation]. Georgia Tech; 2005. Available from: http://hdl.handle.net/1853/7244

Georgia Tech

9. Fonseca, Michael Agapito. Polymer/Ceramic Wireless MEMS Pressure Sensors for Harsh Environments: High Temperature and Biomedical Applications.

Degree: PhD, Electrical and Computer Engineering, 2007, Georgia Tech

URL: http://hdl.handle.net/1853/19789

► This dissertation presents an investigation of miniaturized sensors, designed to wirelessly measure pressure in harsh environments such as high temperature and biomedical applications. Current wireless… (more)

▼ This dissertation presents an investigation of miniaturized sensors, designed to wirelessly measure pressure in harsh environments such as high temperature and biomedical applications. Current wireless MEMS pressure sensors are silicon-based and have limited high temperature operation, require internal power sources, or have limited packaging technology that restricts their use in harsh environments. Sensor designs in this work are based on passive LC resonant circuits to achieve wireless telemetry without the need for active circuitry or internal power sources. A cavity, which is embedded into the substrate, is bound by two pressure-deformable plates that include a parallel-plate capacitor. Deflection of the plates from applied pressure changes the capacitance, thus the resonance frequency varies and is a function of the applied pressure. The LC resonant circuit and pressure-deformable plates are fabricated into a monolithic housing that servers as the final device package (i.e. intrinsically packaged). This co-integration of device and package offers increased robustness and the ability to operate wirelessly in harsh environments. To intrinsically packaged devices, the fabrication approach relies on techniques developed for MEMS and leverage established lamination-based manufacturing processes, such as ceramic and flex-circuit packaging technologies. To demonstrate operation in high temperatures applications, LTCC and HTCC ceramic pressure sensors were fabricated and characterized, operating up to 450°C under 5 bars of pressure while HTCC devices demonstrated electrical functionality up to 600°C. To demonstrate operation in biomedical implantable applications, polymer-based and polymer-ceramic flexible designs were fabricated and characterized. Bench testing for > 300 millions pressure cycles (simulated 7 years of pulse pressure) confirmed the reduction of frequency drift for polymer-ceramic pressure sensors compared to purely polymer-based pressure sensors. Finally, LCP-based pressure sensors were delivered in vivo into canine models with mock abdominal aortic aneurysms and monitored wirelessly over 30 days. The animal results confirmed both catheter deliverability and wireless telemetry in real biomedical applications. Advisors/Committee Members: Dr. Mark G. Allen (Committee Chair), Dr. Oliver Brand (Committee Co-Chair), Dr. Andrew Peterson (Committee Member), Dr. Elliot Chaikof (Committee Member), Dr. Gregory Durgin (Committee Member), Dr. Robert Butera (Committee Member).

Subjects/Keywords: High temperature; Pressure; Sensors; MEMS; Passive; Wireless; Biomedical; Detectors; Biosensors; Extreme environments; Microelectromechanical systems; Wireless communication systems

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fonseca, M. A. (2007). Polymer/Ceramic Wireless MEMS Pressure Sensors for Harsh Environments: High Temperature and Biomedical Applications. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/19789

Chicago Manual of Style (16^th Edition):

Fonseca, Michael Agapito. “Polymer/Ceramic Wireless MEMS Pressure Sensors for Harsh Environments: High Temperature and Biomedical Applications.” 2007. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/19789.

MLA Handbook (7^th Edition):

Fonseca, Michael Agapito. “Polymer/Ceramic Wireless MEMS Pressure Sensors for Harsh Environments: High Temperature and Biomedical Applications.” 2007. Web. 23 Jan 2021.

Vancouver:

Fonseca MA. Polymer/Ceramic Wireless MEMS Pressure Sensors for Harsh Environments: High Temperature and Biomedical Applications. [Internet] [Doctoral dissertation]. Georgia Tech; 2007. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/19789.

Council of Science Editors:

Fonseca MA. Polymer/Ceramic Wireless MEMS Pressure Sensors for Harsh Environments: High Temperature and Biomedical Applications. [Doctoral Dissertation]. Georgia Tech; 2007. Available from: http://hdl.handle.net/1853/19789

Georgia Tech

10. Rose, Stacey Loren. In Vitro Model of Vascular Healing in the Presence of Biomaterials.

Degree: PhD, Biomedical Engineering, 2006, Georgia Tech

URL: http://hdl.handle.net/1853/13955

► Coronary artery stent placement has been a significant advance in the percutaneous treatment of atherosclerotic disease, and tissue engineered vascular grafts may provide a viable… (more)

▼ Coronary artery stent placement has been a significant advance in the percutaneous treatment of atherosclerotic disease, and tissue engineered vascular grafts may provide a viable alternative to autologous segments for small diameter vessels. However, in-stent restenosis remains an important limitation, and tissue engineered grafts have poor patency and high risk of thrombus formation due to their inability to maintain a confluent, adherent, and quiescent endothelium. While animal models provide insight into the pathophysiology of these situations, elucidation of the relative importance of stent or graft components, hemodynamic factors, and molecular factors is difficult. Very little research has focused on bridging gaps in knowledge concerning blood/biomaterial interactions, blood/endothelial cell interactions, and endothelial cell/smooth muscle cell cross-talk. The work presented within this thesis will do just that. The objective of this thesis research was to elucidate the influence of biomaterial-induced activation of leukocytes on endothelial cell or smooth muscle cell phenotype, as well as endothelial cell/smooth muscle cell cross-talk in co-culture systems. Towards this goal, two complimentary in vitro endothelial cell/smooth muscle cell co-culture models with divergent smooth muscle cell phenotype were developed and characterized. Using these systems, it was found that the presence of more secretory smooth muscle cells (as would be seen in wound healing or disease) in general enhanced endothelial cell activation in response to biomaterial-pretreated monocytes, while the presence of less secretory smooth muscle cells (to model more quiescent smooth muscle cells found in uninjured healthy vessels) suppressed endothelial cell activation in response to biomaterial-pretreated monocytes (and neutrophils to a small degree). Additionally, biomaterial-pretreated monocytes and neutrophils amplified a smooth muscle cell phenotypic shift away from a more quiescent state. It is likely that the compounding effect of secretory smooth muscle cells and biomaterial-activated leukocytes are responsible for altered vascular wound healing upon implantation of stents or vascular grafts. Understanding the specific signals causing these effects, or signals delivered by contractile smooth muscle cells that limit these effects help to provide design criteria for development of devices or grafts capable of long term patency. Advisors/Committee Members: Dr. Julia Babensee (Committee Chair), Dr. Elliot Chaikof (Committee Member), Dr. Hanjoong Jo (Committee Member), Dr. Michael Sefton (Committee Member), Dr. Robert Nerem (Committee Member), Dr. Suzanne Eskin (Committee Member).

Subjects/Keywords: Biomaterials; Leukocytes; Smooth muscle cells; Endothelial cells; Stents (Surgery); Vascular endothelial growth factors; Biocompatibility; Leucocytes; Regeneration (Biology)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rose, S. L. (2006). In Vitro Model of Vascular Healing in the Presence of Biomaterials. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/13955

Chicago Manual of Style (16^th Edition):

Rose, Stacey Loren. “In Vitro Model of Vascular Healing in the Presence of Biomaterials.” 2006. Doctoral Dissertation, Georgia Tech. Accessed January 23, 2021. http://hdl.handle.net/1853/13955.

MLA Handbook (7^th Edition):

Rose, Stacey Loren. “In Vitro Model of Vascular Healing in the Presence of Biomaterials.” 2006. Web. 23 Jan 2021.

Vancouver:

Rose SL. In Vitro Model of Vascular Healing in the Presence of Biomaterials. [Internet] [Doctoral dissertation]. Georgia Tech; 2006. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1853/13955.

Council of Science Editors:

Rose SL. In Vitro Model of Vascular Healing in the Presence of Biomaterials. [Doctoral Dissertation]. Georgia Tech; 2006. Available from: http://hdl.handle.net/1853/13955

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations